ORF
Human Ortholog
Description Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.99 0.98 0.94 0.9 – 0.78 0.76 0.61 0.71 1.0 0.99 0.99 0.92 0.87 0.89 0.88 0.9 0.76 0.64 0.69 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.14 – 0.35 0.29 0.41 0.25 0 0 0 0 0 0 0.06 0 0.11 0.17 0.13 0.1
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0.06 0.12 0.11 0 0 0 0.05 0.07 0.06 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 – 1 0 0 0 0 0 0 0 1 0 1 1 6 6 3 9
Bud 0 1 0 1 0 4 3 – 13 11 7 8 3 1 0 0 1 0 0 5 1 9 4 17
Bud Neck 1 2 16 15 1 1 1 – 3 1 0 1 0 1 0 0 0 0 0 1 1 6 5 6
Bud Site 0 0 0 0 0 0 0 – 2 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 5 5 4 0 – 2 4 1 2 1 0 1 8 11 7 0 1 8 6 6 7
Cytoplasm 162 376 374 343 320 404 425 – 363 394 286 379 526 604 667 344 243 235 143 340 270 236 270 359
Endoplasmic Reticulum 0 0 4 0 2 0 7 – 0 2 0 0 0 1 1 13 10 9 0 0 1 5 6 8
Endosome 0 0 1 0 0 0 0 – 0 0 0 6 1 2 0 13 15 9 1 0 4 3 3 12
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 2 0 1 2 0 1 1
Mitochondria 3 3 0 0 3 54 66 – 163 152 190 136 5 4 3 4 2 5 9 8 37 61 50 54
Nucleus 0 1 0 0 0 3 3 – 5 9 4 11 0 1 2 2 2 2 0 2 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 – 0 0 2 0 0 1 1 1 0 1 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 1 0 2 0 3 3 5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 2 1 1 0 0 – 3 0 3 7 0 1 0 9 4 5 0 1 5 5 8 12
Vac/Vac Membrane 1 1 1 0 1 5 5 – 16 31 55 57 0 2 0 19 20 16 2 4 1 15 19 21
Unique Cell Count 165 378 384 346 325 430 471 467 520 469 537 528 610 672 375 278 263 163 377 355 369 390 527
Labelled Cell Count 167 385 400 366 333 475 511 571 604 549 608 536 622 676 416 314 296 163 377 355 369 390 527


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.8 4.7 4.4 5.3 4.4 4.6 – 4.2 4.4 4.2 4.8 4.0 4.3 5.0 7.1 7.7 7.4 3.8 3.9 4.3
Std Deviation (1e-4) 0.9 0.8 0.9 1.0 1.3 1.1 0.8 – 1.0 1.1 1.1 1.2 0.9 0.9 1.1 1.3 1.5 1.7 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.08 0.19 -0.08 -0.03 – -0.17 -0.1 -0.14 0.04 -0.21 -0.12 0.1 0.6 0.73 0.65 -0.31 -0.27 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.1 5.0 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4561 0.0801 -0.1365 -0.6542 -0.6313 -0.3469 0.6822 0.2355 -0.0005 0.7373 0.0049 0.2885 -0.4454 0.4832 0.1876 0.2552 -0.0782 0.1873
Actin 0.0005 0.0004 0.0008 0.0001 0.0003 0.0003 0.0377 0.0012 0.0286 0.0367 0.0083 0.007 0.0038 0.0004 0.0007 0.0001 0.0569 0.0001
Bud 0.0001 0.0012 0.0003 0.0001 0 0.0002 0.0007 0.0025 0.0029 0.0042 0.0003 0.004 0.0001 0.0004 0.0011 0.0004 0.0004 0.0001
Bud Neck 0.0001 0.0001 0.0002 0 0.0001 0.0006 0.003 0.0005 0.0006 0.0015 0.0006 0.0048 0.0001 0.0001 0.0002 0.0001 0.0035 0.0003
Bud Periphery 0.0001 0.0013 0.0002 0.0001 0 0.0002 0.0019 0.004 0.0033 0.0114 0.0005 0.0015 0.0001 0.0003 0.0028 0.0003 0.0004 0.0001
Bud Site 0.0005 0.0052 0.0008 0.0001 0 0.0001 0.0041 0.0103 0.0026 0.0182 0.0009 0.003 0.0002 0.002 0.0033 0.0002 0.0059 0
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0002 0.0003 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0
Cytoplasm 0.0939 0.1553 0.1159 0.0955 0.1151 0.1613 0.0656 0.1276 0.1049 0.1073 0.0942 0.2042 0.1429 0.3448 0.3142 0.2493 0.2115 0.39
Cytoplasmic Foci 0.0064 0.005 0.005 0.0043 0.0073 0.0152 0.018 0.01 0.007 0.0148 0.016 0.0146 0.0146 0.0127 0.0074 0.0064 0.0209 0.0063
Eisosomes 0.0001 0.0002 0.0002 0 0.0001 0 0.0004 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0009 0.0011 0.0008 0.0006 0.0006 0.0013 0.0025 0.002 0.0011 0.0076 0.0017 0.0011 0.0014 0.0018 0.0013 0.0016 0.0012 0.0017
Endosome 0.0013 0.0024 0.0016 0.0017 0.0018 0.0063 0.0117 0.0022 0.0029 0.0306 0.0095 0.0069 0.0068 0.0032 0.0033 0.0039 0.0031 0.0035
Golgi 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0075 0.0003 0.003 0.0107 0.0165 0.0023 0.001 0.0003 0.0005 0.0002 0.0007 0.0001
Lipid Particles 0.0006 0.0002 0.0001 0.0001 0.0002 0.0005 0.0168 0.0005 0.002 0.0073 0.0321 0.003 0.003 0.0003 0.0006 0.0001 0.0023 0.0001
Mitochondria 0.0002 0.0004 0.0003 0.0003 0.0002 0.0002 0.0254 0.0009 0.007 0.0128 0.0123 0.0012 0.001 0.0009 0.0096 0.0006 0.0003 0.0001
None 0.8915 0.8209 0.8679 0.8925 0.871 0.8003 0.7338 0.8266 0.8218 0.644 0.789 0.7224 0.8119 0.6283 0.649 0.7326 0.6707 0.595
Nuclear Periphery 0.0003 0.0006 0.0005 0.0007 0.0004 0.001 0.014 0.0013 0.0023 0.017 0.0003 0.0047 0.003 0.0006 0.0006 0.0006 0.0003 0.0006
Nucleolus 0.0002 0.0003 0.0004 0.0002 0.0001 0.0003 0.0007 0.0005 0.0004 0.0023 0.0001 0.0005 0.0004 0.0001 0.0002 0.0001 0.0088 0
Nucleus 0.0013 0.0025 0.0021 0.0014 0.0008 0.0043 0.0201 0.0025 0.0017 0.0189 0.0002 0.0082 0.0022 0.0012 0.0011 0.0012 0.0029 0.0009
Peroxisomes 0.0004 0.0001 0.0002 0.0001 0.0001 0.0007 0.0212 0.001 0.0028 0.0101 0.016 0.0024 0.0029 0.0002 0.0007 0.0001 0.0013 0.0001
Punctate Nuclear 0.0007 0.0009 0.0013 0.0012 0.0011 0.0064 0.0107 0.0029 0.0038 0.0272 0.0006 0.0069 0.0034 0.001 0.0013 0.0005 0.0046 0.0004
Vacuole 0.0006 0.0014 0.0011 0.0008 0.0004 0.0008 0.0023 0.002 0.0009 0.0118 0.0006 0.0007 0.0006 0.001 0.0013 0.0015 0.0037 0.0006
Vacuole Periphery 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0016 0.0005 0.0002 0.0047 0.0003 0.0003 0.0003 0.0001 0.0004 0.0004 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.2642 0.8996 1.6688 5.2662 1.3047 8.0459 3.9793 9.0548 10.3343 4.8417
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1329 1269 194 1231 1393 1568 1623 129 2722 2837 1817 1360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 588.30 694.05 1006.34 809.29 625.97 692.16 782.24 843.18 607.58 693.01 806.17 812.50
Standard Deviation 81.34 89.96 120.39 123.18 66.26 85.92 98.16 108.38 76.36 87.75 122.24 122.26
Intensity Change Log 2 — 0.238488 0.774494 0.460105 — 0.145012 0.321518 0.429747 — 0.191057 0.558725 0.444535

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000062 0.000352 0.000521 0.001233 0.000103 0.000611 0.000208 0.000247 0.000083 0.000495 0.000242 0.001140
Bud Neck 0.025726 0.037897 0.002077 0.008817 0.020279 0.045972 0.004148 0.006628 0.022938 0.042360 0.003927 0.008609
Bud Site 0.004386 0.010536 0.003186 0.035679 0.002710 0.017563 0.008266 0.008765 0.003528 0.014420 0.007724 0.033127
Cell Periphery 0.000263 0.000141 0.000293 0.000140 0.000116 0.000172 0.000118 0.000241 0.000188 0.000158 0.000137 0.000149
Cytoplasm 0.459770* 0.391603* 0.336344* 0.471731* 0.460400* 0.344287* 0.448644* 0.433485* 0.460092* 0.365452* 0.436654* 0.468104*
Cytoplasmic Foci 0.167151 0.242824* 0.030596 0.018294 0.227637* 0.297320* 0.013125 0.061625 0.198105* 0.272944* 0.014990 0.022404
Eisosomes 0.000285 0.000141 0.000116 0.000035 0.000179 0.000200 0.000033 0.000099 0.000231 0.000173 0.000042 0.000041
Endoplasmic Reticulum 0.001380 0.000910 0.007508 0.001828 0.000498 0.000430 0.001807 0.001701 0.000929 0.000645 0.002415 0.001816
Endosome 0.010067 0.026481 0.007800 0.005284 0.008295 0.036935 0.001765 0.024981 0.009160 0.032259 0.002410 0.007153
Golgi 0.001383 0.004514 0.000163 0.000989 0.001804 0.010611 0.000407 0.003089 0.001598 0.007884 0.000381 0.001188
Lipid Particles 0.011683 0.007340 0.004362 0.001211 0.009862 0.011399 0.001089 0.002564 0.010751 0.009583 0.001439 0.001339
Mitochondria 0.005588 0.015834 0.000397 0.002298 0.005439 0.028191 0.000918 0.000675 0.005511 0.022663 0.000863 0.002144
Mitotic Spindle 0.001719 0.007411 0.001710 0.097278 0.000451 0.015322 0.011140 0.008638 0.001070 0.011783 0.010133 0.088870
None 0.008117 0.002349 0.002536 0.007979 0.002341 0.002233 0.008005 0.007478 0.005161 0.002285 0.007421 0.007932
Nuclear Periphery 0.000609 0.000592 0.001970 0.002048 0.000511 0.000311 0.001981 0.001191 0.000559 0.000437 0.001980 0.001967
Nuclear Periphery Foci 0.000205 0.000484 0.003977 0.001612 0.000209 0.000234 0.000605 0.001398 0.000208 0.000346 0.000965 0.001592
Nucleolus 0.001555 0.001749 0.000740 0.000860 0.000993 0.001986 0.000432 0.001704 0.001267 0.001880 0.000465 0.000940
Nucleus 0.247581* 0.146620* 0.420286* 0.260928* 0.210081* 0.079352 0.444929* 0.298780* 0.228391* 0.109441 0.442298* 0.264518*
Peroxisomes 0.002611 0.022120 0.000453 0.003228 0.007440 0.029223 0.001317 0.005689 0.005082 0.026046 0.001225 0.003462
Vacuole 0.048618 0.077881 0.174625 0.075563 0.040069 0.074500 0.050702 0.130249 0.044243 0.076012 0.063933 0.080750
Vacuole Periphery 0.001242 0.002221 0.000339 0.002965 0.000584 0.003150 0.000359 0.000772 0.000906 0.002735 0.000357 0.002757

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.37 -10.01 -4.41 -2.58 -0.37 -4.01 -2.88 -4.12 1.27 -2.14 -5.70 -5.05 -4.49 -1.66 -3.07
Bud Neck -3.68 11.72 5.57 8.73 -10.99 -9.03 9.70 5.58 12.70 -0.96 -9.03 14.01 6.55 14.84 -8.68
Bud Site -4.88 -0.19 -8.02 -5.50 -8.32 -10.35 -2.62 -2.90 0.70 -2.07 -11.08 -1.98 -9.07 -3.79 -7.45
Cell Periphery 6.20 -0.26 8.94 1.07 5.43 -4.33 1.41 -1.83 -0.02 -2.24 2.65 6.81 6.07 2.82 -0.62
Cytoplasm 6.85 10.95 11.95 4.60 -4.15 11.90 9.97 5.44 0.86 1.63 13.50 13.45 14.10 3.07 1.65
Cytoplasmic Foci -9.58 26.28 29.70 34.39 -0.40 -8.33 37.83 20.24 27.55 -5.31 -12.88 48.73 43.23 54.48 -7.07
Eisosomes 6.39 7.19 10.45 7.49 5.51 -1.37 16.45 4.47 5.07 -7.88 4.43 16.09 14.06 12.52 -6.24
Endoplasmic Reticulum 1.46 -9.19 -7.63 -10.30 6.98 1.09 -17.30 -6.24 -6.43 0.87 1.82 -13.62 -13.22 -15.88 0.73
Endosome -7.67 2.27 3.95 10.36 1.85 -14.09 10.56 -1.29 3.58 -2.49 -15.59 12.19 2.66 16.48 -6.29
Golgi -4.89 7.45 4.28 6.36 -3.71 -7.82 6.85 0.37 6.34 -1.35 -9.13 7.82 5.39 10.52 -2.40
Lipid Particles 4.00 6.87 9.37 9.56 4.02 -1.39 11.73 8.06 8.50 -5.19 1.39 15.02 13.31 12.75 -6.83
Mitochondria -4.54 5.56 4.35 7.15 -6.69 -8.98 4.74 4.94 11.87 1.36 -9.96 7.02 5.94 13.49 -4.20
Mitotic Spindle -3.20 -0.49 -13.63 -12.45 -13.48 -6.96 -5.67 -3.11 -1.62 -2.09 -7.52 -5.29 -14.12 -11.75 -11.95
None 4.44 4.68 -0.27 -8.60 -8.96 -0.19 -4.10 -2.11 -2.05 -0.72 3.94 -0.32 -3.57 -8.41 -2.99
Nuclear Periphery -0.19 -11.39 -18.20 -19.19 -7.79 3.35 -17.65 -5.10 -5.82 3.23 1.82 -18.71 -19.76 -20.83 -5.97
Nuclear Periphery Foci -2.73 -9.03 -6.88 -6.43 -0.31 -1.41 -5.94 -2.17 -2.15 -2.02 -3.09 -9.42 -6.97 -6.75 -5.51
Nucleolus -0.83 5.12 -4.47 -4.03 -6.43 -3.26 4.02 -1.67 -1.42 -1.78 -3.08 6.55 -4.95 -3.76 -6.17
Nucleus 12.70 -8.11 -3.55 -15.15 6.48 21.62 -31.36 -2.06 -7.91 9.91 24.01 -32.00 -6.38 -22.77 21.05
Peroxisomes -10.96 12.58 -0.81 10.07 -4.30 -10.46 7.10 0.24 5.76 -1.68 -15.08 7.62 2.03 15.31 -3.59
Vacuole -8.29 -11.01 -20.40 -14.46 2.92 -10.47 -13.47 -9.13 -7.60 -6.61 -13.05 -15.93 -23.29 -17.49 -11.07
Vacuole Periphery -2.99 4.60 0.75 2.84 -1.69 -8.75 4.11 0.87 7.78 -0.87 -8.34 5.59 0.07 4.76 -1.94
ORF
Human Ortholog
Description Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR149C

YLR149C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr149c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available