Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 11 | 14 | 11 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 3 | 8 | 10 | 12 | 8 | 19 | 3 | 6 | 2 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 109 | 167 | 239 | 155 | 160 | 212 | 208 | 161 | 210 | 152 | 63 | 70 | 192 | 281 | 269 | 184 | 146 | 120 | 107 | 152 | 198 | 140 | 194 | 261 |
Endoplasmic Reticulum | 0 | 2 | 3 | 4 | 9 | 26 | 43 | 46 | 31 | 48 | 17 | 11 | 3 | 1 | 3 | 11 | 11 | 10 | 1 | 1 | 9 | 5 | 6 | 12 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 2 | 5 | 16 | 26 | 18 | 32 | 19 | 8 | 1 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 4 | 12 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 2 | 2 | 0 | 2 | 4 | 0 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 0 | 5 | 7 | 8 |
Unique Cell Count | 109 | 169 | 242 | 161 | 176 | 245 | 276 | 246 | 271 | 238 | 101 | 103 | 196 | 286 | 275 | 190 | 149 | 123 | 111 | 163 | 217 | 163 | 225 | 294 |
Labelled Cell Count | 110 | 169 | 246 | 162 | 178 | 253 | 288 | 264 | 290 | 271 | 114 | 119 | 198 | 286 | 279 | 200 | 161 | 133 | 111 | 163 | 217 | 163 | 225 | 294 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 189.1 | 156.1 | 139.3 | 125.6 | 76.1 | 78.1 | 67.9 | 61.1 | 58.2 | 59.0 | 54.5 | 49.4 | 149.7 | 150.6 | 133.0 | 152.9 | 177.3 | 177.1 | 150.1 | 165.3 | 165.8 |
Std Deviation (1e-4) | 43.3 | 33.3 | 33.5 | 35.9 | 26.9 | 22.0 | 20.4 | 18.9 | 16.9 | 17.1 | 18.5 | 13.7 | 44.2 | 38.6 | 40.2 | 45.9 | 62.9 | 72.3 | 48.4 | 51.4 | 51.6 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.87 | -0.83 | -1.04 | -1.19 | -1.26 | -1.24 | -1.35 | -1.5 | 0.1 | 0.11 | -0.07 | 0.14 | 0.35 | 0.35 | 0.11 | 0.25 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.3 | 3.6 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 2.1 | 2.7 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -3.8 | -5.2 | -7.7 | -9.6 | -7.3 | -9.8 | -9.5 | -8.6 | -0.7 | -0.5 | -0.8 | -1.4 | -0.6 | -0.9 |
Endoplasmic Reticulum | 0 | 2.3 | 4.4 | 5.7 | 6.4 | 4.6 | 6.7 | 5.6 | 0 | 0 | 0 | 0 | 2.7 | 3.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 3.8 | 5.2 | 4.1 | 5.9 | 6.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 144.2422 | 184.8548 | 163.3948 | 171.3258 | 111.631 | 108.6535 | 185.9413 | 232.1701 | 207.9186 | 197.4994 | 199.948 | 109.5544 | 152.0069 | 200.8201 | 182.0814 | 159.5784 | 168.0825 | 177.6941 |
Actin | 0.0044 | 0.0008 | 0.0254 | 0.0005 | 0.0542 | 0.0057 | 0.001 | 0.0005 | 0.005 | 0.0004 | 0.0058 | 0.004 | 0.0075 | 0.0083 | 0.018 | 0.0044 | 0.0015 | 0.0045 |
Bud | 0.0031 | 0.0024 | 0.004 | 0.0026 | 0.0031 | 0.0051 | 0.003 | 0.0018 | 0.003 | 0.005 | 0.0056 | 0.0069 | 0.0052 | 0.004 | 0.0072 | 0.0077 | 0.0067 | 0.007 |
Bud Neck | 0.0016 | 0.0015 | 0.0018 | 0.0007 | 0.0014 | 0.0027 | 0.0009 | 0.0012 | 0.0011 | 0.0008 | 0.0013 | 0.0014 | 0.0011 | 0.0022 | 0.0022 | 0.002 | 0.0021 | 0.0016 |
Bud Periphery | 0.0046 | 0.0018 | 0.0039 | 0.0018 | 0.0024 | 0.0036 | 0.0039 | 0.0012 | 0.0031 | 0.0035 | 0.0063 | 0.0036 | 0.0093 | 0.0029 | 0.006 | 0.0089 | 0.0042 | 0.0063 |
Bud Site | 0.0041 | 0.0017 | 0.0093 | 0.0005 | 0.0076 | 0.0014 | 0.0007 | 0.0038 | 0.0011 | 0.0008 | 0.0008 | 0.0025 | 0.0023 | 0.0052 | 0.0024 | 0.0057 | 0.0017 | 0.0012 |
Cell Periphery | 0.0663 | 0.0471 | 0.0665 | 0.0644 | 0.0155 | 0.0333 | 0.0407 | 0.0162 | 0.039 | 0.0226 | 0.0226 | 0.0095 | 0.0825 | 0.0496 | 0.0596 | 0.0918 | 0.0458 | 0.0926 |
Cytoplasm | 0.3375 | 0.5833 | 0.3348 | 0.263 | 0.2009 | 0.4297 | 0.5252 | 0.7684 | 0.5753 | 0.417 | 0.4886 | 0.2794 | 0.4144 | 0.71 | 0.5255 | 0.2402 | 0.3186 | 0.4392 |
Cytoplasmic Foci | 0.0129 | 0.0122 | 0.0058 | 0.0024 | 0.0356 | 0.0475 | 0.0032 | 0.0023 | 0.0062 | 0.0027 | 0.011 | 0.0996 | 0.0055 | 0.0024 | 0.0042 | 0.0016 | 0.0272 | 0.0025 |
Eisosomes | 0.0076 | 0.0001 | 0.0016 | 0.0001 | 0.0009 | 0.0011 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0009 | 0.0016 | 0.0036 | 0.0001 | 0.0014 | 0.0028 | 0.0001 |
Endoplasmic Reticulum | 0.0542 | 0.0191 | 0.0143 | 0.0016 | 0.0018 | 0.0115 | 0.0409 | 0.0192 | 0.0186 | 0.0137 | 0.0222 | 0.0361 | 0.0183 | 0.0066 | 0.0104 | 0.0103 | 0.0053 | 0.0052 |
Endosome | 0.0131 | 0.0074 | 0.0185 | 0.0123 | 0.0175 | 0.0295 | 0.018 | 0.0122 | 0.0166 | 0.0144 | 0.0469 | 0.0734 | 0.0142 | 0.0039 | 0.0239 | 0.0153 | 0.0215 | 0.0051 |
Golgi | 0.0054 | 0.001 | 0.01 | 0.0008 | 0.0073 | 0.0126 | 0.0032 | 0.0017 | 0.0029 | 0.0004 | 0.0091 | 0.019 | 0.0022 | 0.0022 | 0.0038 | 0.0048 | 0.0027 | 0.001 |
Lipid Particles | 0.0074 | 0.002 | 0.0056 | 0.0008 | 0.0127 | 0.0091 | 0.0016 | 0.0005 | 0.0022 | 0.0009 | 0.0042 | 0.061 | 0.0041 | 0.003 | 0.0024 | 0.0058 | 0.0082 | 0.0086 |
Mitochondria | 0.0312 | 0.0072 | 0.0168 | 0.0016 | 0.0573 | 0.0397 | 0.0134 | 0.0103 | 0.0147 | 0.0055 | 0.0191 | 0.0907 | 0.0339 | 0.0045 | 0.0346 | 0.0169 | 0.0268 | 0.0038 |
None | 0.006 | 0.001 | 0.0022 | 0.0002 | 0.0204 | 0.0197 | 0.002 | 0.0003 | 0.0016 | 0.003 | 0.0014 | 0.0384 | 0.0108 | 0.004 | 0.0014 | 0.0016 | 0.0126 | 0.0008 |
Nuclear Periphery | 0.0574 | 0.0189 | 0.0167 | 0.0076 | 0.0135 | 0.0112 | 0.0222 | 0.0041 | 0.0112 | 0.006 | 0.0107 | 0.0127 | 0.0361 | 0.0115 | 0.0135 | 0.0368 | 0.0293 | 0.0264 |
Nucleolus | 0.0143 | 0.0021 | 0.007 | 0.0028 | 0.0317 | 0.0901 | 0.0046 | 0.0006 | 0.0023 | 0.0041 | 0.0017 | 0.0174 | 0.0138 | 0.0047 | 0.004 | 0.011 | 0.0404 | 0.0039 |
Nucleus | 0.0186 | 0.0077 | 0.0102 | 0.0117 | 0.0181 | 0.0178 | 0.009 | 0.0029 | 0.0061 | 0.006 | 0.0056 | 0.0129 | 0.0294 | 0.01 | 0.0132 | 0.0232 | 0.0189 | 0.0179 |
Peroxisomes | 0.0041 | 0.0003 | 0.0019 | 0.0001 | 0.0028 | 0.0018 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0016 | 0.0041 | 0.0006 | 0.0006 | 0.0014 | 0.0006 | 0.0023 | 0.0003 |
Punctate Nuclear | 0.0004 | 0.0001 | 0.001 | 0 | 0.0004 | 0.0021 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.001 | 0.0054 | 0.0013 | 0.0003 | 0.0002 | 0.0005 | 0.0008 | 0.0002 |
Vacuole | 0.2267 | 0.2177 | 0.3159 | 0.436 | 0.2571 | 0.1313 | 0.22 | 0.1229 | 0.2221 | 0.3518 | 0.2345 | 0.121 | 0.2274 | 0.136 | 0.2023 | 0.3799 | 0.2718 | 0.2639 |
Vacuole Periphery | 0.1189 | 0.0646 | 0.1268 | 0.1885 | 0.2377 | 0.0935 | 0.0858 | 0.0292 | 0.0665 | 0.1409 | 0.1 | 0.1 | 0.0787 | 0.0247 | 0.0636 | 0.1295 | 0.1488 | 0.108 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1840.3497 | 1963.1526 | 2206.4862 | 1743.0561 | 2168.5434 | 1986.9067 | 1691.0222 | 1693.0522 | 1827.5761 | 1405.6159 |
Translational Efficiency | 2.0463 | 1.9741 | 1.7468 | 2.2142 | 1.9938 | 1.9305 | 1.7884 | 2.1941 | 1.7882 | 2.1175 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
84 | 752 | 2275 | 1751 | 1995 | 1907 | 1289 | 1774 | 2079 | 2659 | 3564 | 3525 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 7466.29 | 8649.53 | 12354.29 | 13083.25 | 8440.58 | 9926.40 | 12236.86 | 13966.02 | 8401.21 | 9565.28 | 12311.82 | 13527.51 |
Standard Deviation | 2162.11 | 2025.97 | 2747.51 | 3926.51 | 1851.08 | 2290.51 | 3550.61 | 3534.90 | 1874.49 | 2292.21 | 3062.90 | 3760.56 |
Intensity Change Log 2 | — | 0.212230 | 0.726549 | 0.809258 | — | 0.233928 | 0.535819 | 0.726507 | — | 0.223784 | 0.628489 | 0.765940 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000214 | 0.000094 | 0.000042 | 0.000310 | 0.000032 | 0.000076 | 0.000150 | 0.000153 | 0.000040 | 0.000081 | 0.000081 | 0.000231 |
Bud Neck | 0.000439 | 0.000664 | 0.000633 | 0.001363 | 0.000276 | 0.000313 | 0.002234 | 0.000689 | 0.000282 | 0.000412 | 0.001212 | 0.001023 |
Bud Site | 0.003596 | 0.003145 | 0.002927 | 0.010675 | 0.001960 | 0.002405 | 0.003706 | 0.007192 | 0.002026 | 0.002615 | 0.003209 | 0.008922 |
Cell Periphery | 0.079408 | 0.028865 | 0.006642 | 0.022094 | 0.005878 | 0.010967 | 0.012583 | 0.034291 | 0.008849 | 0.016028 | 0.008791 | 0.028232 |
Cytoplasm | 0.043510 | 0.348460* | 0.437063* | 0.393849* | 0.456801* | 0.492707* | 0.380683* | 0.350736* | 0.440102* | 0.451912* | 0.416672* | 0.372152* |
Cytoplasmic Foci | 0.007447 | 0.012056 | 0.005466 | 0.007751 | 0.016855 | 0.018420 | 0.010475 | 0.009159 | 0.016475 | 0.016620 | 0.007277 | 0.008460 |
Eisosomes | 0.000011 | 0.000003 | 0.000001 | 0.000004 | 0.000001 | 0.000002 | 0.000003 | 0.000003 | 0.000001 | 0.000002 | 0.000002 | 0.000004 |
Endoplasmic Reticulum | 0.002847 | 0.000852 | 0.000648 | 0.000832 | 0.000914 | 0.001039 | 0.000541 | 0.001147 | 0.000992 | 0.000986 | 0.000609 | 0.000990 |
Endosome | 0.001250 | 0.001073 | 0.000828 | 0.001173 | 0.000765 | 0.000916 | 0.000789 | 0.001152 | 0.000785 | 0.000961 | 0.000814 | 0.001162 |
Golgi | 0.287594* | 0.132726* | 0.031753 | 0.037907 | 0.108184 | 0.134429* | 0.061312 | 0.075907 | 0.115433* | 0.133947* | 0.042444 | 0.057031 |
Lipid Particles | 0.048819 | 0.024164 | 0.004701 | 0.012236 | 0.004042 | 0.010723 | 0.009685 | 0.017376 | 0.005851 | 0.014524 | 0.006504 | 0.014822 |
Mitochondria | 0.000478 | 0.000227 | 0.000304 | 0.001467 | 0.000402 | 0.000423 | 0.000582 | 0.000595 | 0.000405 | 0.000367 | 0.000404 | 0.001028 |
Mitotic Spindle | 0.000896 | 0.002014 | 0.000535 | 0.001291 | 0.001430 | 0.001138 | 0.000945 | 0.000790 | 0.001409 | 0.001386 | 0.000683 | 0.001039 |
None | 0.000556 | 0.002729 | 0.001459 | 0.003053 | 0.002126 | 0.004344 | 0.002877 | 0.001695 | 0.002063 | 0.003887 | 0.001972 | 0.002370 |
Nuclear Periphery | 0.001305 | 0.001155 | 0.000651 | 0.000461 | 0.000797 | 0.000783 | 0.001663 | 0.000644 | 0.000817 | 0.000889 | 0.001017 | 0.000553 |
Nuclear Periphery Foci | 0.007051 | 0.019579 | 0.006008 | 0.004881 | 0.010819 | 0.011259 | 0.012584 | 0.006866 | 0.010667 | 0.013612 | 0.008386 | 0.005880 |
Nucleolus | 0.000097 | 0.000181 | 0.000196 | 0.000323 | 0.000081 | 0.000218 | 0.000302 | 0.000250 | 0.000082 | 0.000208 | 0.000234 | 0.000286 |
Nucleus | 0.009951 | 0.005588 | 0.008204 | 0.008227 | 0.002633 | 0.003421 | 0.030730 | 0.004711 | 0.002929 | 0.004033 | 0.016351 | 0.006457 |
Peroxisomes | 0.000028 | 0.000021 | 0.000040 | 0.000151 | 0.000011 | 0.000021 | 0.000016 | 0.000071 | 0.000012 | 0.000021 | 0.000031 | 0.000111 |
Vacuole | 0.223569* | 0.213579* | 0.302849* | 0.349769* | 0.074836 | 0.101925 | 0.298639* | 0.279214* | 0.080846 | 0.133502 | 0.301326* | 0.314261* |
Vacuole Periphery | 0.280934* | 0.202824* | 0.189051* | 0.142184* | 0.311154* | 0.204472* | 0.169500* | 0.207360* | 0.309933* | 0.204006* | 0.181980* | 0.174985* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.06 | 1.43 | -0.99 | -3.94 | -4.69 | -2.50 | -7.86 | -4.96 | -2.98 | 0.90 | -2.77 | -5.30 | -6.00 | -4.87 | -4.49 |
Bud Neck | -1.55 | -3.20 | -5.36 | -4.93 | -3.67 | -0.22 | -9.28 | -4.37 | -5.27 | 8.14 | -1.47 | -10.27 | -7.13 | -7.02 | 3.67 |
Bud Site | 0.41 | 0.40 | -3.09 | -8.80 | -9.80 | -1.17 | -5.21 | -6.87 | -6.26 | -3.34 | -1.67 | -5.01 | -12.27 | -11.33 | -9.59 |
Cell Periphery | 4.32 | 6.34 | 5.03 | 3.55 | -11.86 | -8.10 | -7.13 | -17.63 | -13.75 | -12.59 | -7.81 | 0.72 | -16.02 | -9.55 | -18.07 |
Cytoplasm | -15.03 | -22.67 | -19.96 | -3.09 | 3.71 | -3.08 | 6.41 | 9.23 | 12.20 | 2.10 | -1.06 | 2.59 | 6.84 | 8.60 | 5.07 |
Cytoplasmic Foci | -1.64 | 0.88 | -0.25 | 3.13 | -3.51 | -1.25 | 2.87 | 6.81 | 7.93 | 2.44 | -0.25 | 7.86 | 7.25 | 8.61 | -1.14 |
Eisosomes | 2.10 | 2.50 | 1.52 | -3.08 | -5.34 | -2.29 | -8.24 | -6.17 | -4.46 | 1.12 | -1.99 | -2.96 | -6.41 | -5.56 | -4.73 |
Endoplasmic Reticulum | 2.60 | 2.92 | 2.68 | 0.47 | -1.70 | -1.16 | 4.44 | -1.95 | -0.81 | -6.44 | 0.10 | 4.79 | 0.36 | 0.30 | -5.34 |
Endosome | 1.08 | 2.57 | 0.51 | -0.68 | -2.70 | -2.26 | -1.54 | -2.92 | -1.76 | -2.04 | -2.84 | -1.19 | -3.89 | -2.11 | -3.40 |
Golgi | 6.10 | 10.33 | 10.09 | 18.70 | -2.89 | -5.85 | 15.49 | 9.32 | 14.61 | -6.40 | -4.39 | 24.15 | 18.13 | 24.89 | -9.11 |
Lipid Particles | 2.71 | 5.01 | 4.17 | 6.56 | -9.01 | -8.94 | -8.34 | -14.32 | -5.86 | -7.32 | -10.12 | -1.07 | -11.60 | 0.01 | -12.59 |
Mitochondria | 0.68 | 0.49 | -1.85 | -4.49 | -4.21 | -0.32 | -2.91 | -2.78 | -2.18 | 0.74 | 0.66 | -0.93 | -4.37 | -4.60 | -4.07 |
Mitotic Spindle | -1.31 | 1.21 | -1.52 | 0.39 | -2.12 | 1.81 | 2.58 | 2.24 | 1.20 | 0.41 | 0.05 | 3.99 | 0.47 | 0.34 | -1.93 |
None | -4.64 | -2.12 | -3.94 | -0.56 | -2.33 | -3.07 | -1.68 | 1.16 | 3.29 | 2.07 | -3.40 | 0.30 | -0.99 | 2.26 | -0.98 |
Nuclear Periphery | 0.60 | 2.73 | 3.98 | 7.37 | 2.06 | 0.12 | -6.96 | 2.62 | 1.15 | 8.14 | -0.74 | -3.46 | 4.42 | 3.52 | 6.68 |
Nuclear Periphery Foci | -8.19 | 0.77 | 2.01 | 11.73 | 2.86 | -0.52 | -1.69 | 6.19 | 7.77 | 6.46 | -3.83 | 3.30 | 8.00 | 14.26 | 6.18 |
Nucleolus | -1.61 | -3.46 | -6.01 | -3.40 | -4.25 | -1.04 | -7.61 | -1.94 | -0.20 | 1.24 | -1.31 | -11.00 | -4.58 | -0.81 | -0.59 |
Nucleus | 0.73 | -0.25 | -0.15 | -4.16 | 0.44 | -2.00 | -13.87 | -4.90 | -3.48 | 12.48 | -2.48 | -15.87 | -8.08 | -6.42 | 10.61 |
Peroxisomes | 0.53 | -0.48 | -3.58 | -4.34 | -3.70 | -2.24 | -3.40 | -5.97 | -4.56 | -5.19 | -2.67 | -4.42 | -6.36 | -5.63 | -4.96 |
Vacuole | 0.39 | -3.78 | -5.61 | -13.32 | -4.96 | -6.51 | -30.24 | -34.18 | -28.11 | 2.77 | -12.24 | -43.90 | -46.26 | -33.24 | -1.33 |
Vacuole Periphery | 3.30 | 4.37 | 6.37 | 8.87 | 8.23 | 14.24 | 21.50 | 14.95 | 0.31 | -8.56 | 15.41 | 21.63 | 22.02 | 7.44 | -0.08 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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