Standard name
Human Ortholog
Description Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.96 0.91 0.87 0.75 0.65 0.77 0.64 0.62 0.68 0.98 0.98 0.98 0.97 0.98 0.98 0.97 0.93 0.91 0.86 0.87 0.89
Endoplasmic Reticulum 0 0 0 0 0.05 0.11 0.16 0.19 0.11 0.2 0.17 0.11 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.06 0.11 0.07 0.13 0.19 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 9 11 14 11 6 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 2 1 0 0 0 3 1 1 0 0 1 1 0 0 0 0 0 2 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 1 3 8 10 12 8 19 3 6 2 2 3 1 2 0 0 0 1 0 0 0
Cytoplasm 109 167 239 155 160 212 208 161 210 152 63 70 192 281 269 184 146 120 107 152 198 140 194 261
Endoplasmic Reticulum 0 2 3 4 9 26 43 46 31 48 17 11 3 1 3 11 11 10 1 1 9 5 6 12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2 5 16 26 18 32 19 8 1 2 1 1 1 2 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 0 3 6 4 4 12 0 0 0 2 0 0 0 0 0 0 1 0
Nucleus 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 2 2 0 2 4 0 2 1 3 0 0 1 0 1 0 0 4 0 5 7 8
Unique Cell Count 109 169 242 161 176 245 276 246 271 238 101 103 196 286 275 190 149 123 111 163 217 163 225 294
Labelled Cell Count 110 169 246 162 178 253 288 264 290 271 114 119 198 286 279 200 161 133 111 163 217 163 225 294


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 189.1 156.1 139.3 125.6 76.1 78.1 67.9 61.1 58.2 59.0 54.5 49.4 149.7 150.6 133.0 152.9 177.3 177.1 150.1 165.3 165.8
Std Deviation (1e-4) 43.3 33.3 33.5 35.9 26.9 22.0 20.4 18.9 16.9 17.1 18.5 13.7 44.2 38.6 40.2 45.9 62.9 72.3 48.4 51.4 51.6
Intensity Change (Log2) -0.15 -0.87 -0.83 -1.04 -1.19 -1.26 -1.24 -1.35 -1.5 0.1 0.11 -0.07 0.14 0.35 0.35 0.11 0.25 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.3 3.6 3.4 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2.1 2.7 0 3.8 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -3.8 -5.2 -7.7 -9.6 -7.3 -9.8 -9.5 -8.6 -0.7 -0.5 -0.8 -1.4 -0.6 -0.9
Endoplasmic Reticulum 0 2.3 4.4 5.7 6.4 4.6 6.7 5.6 0 0 0 0 2.7 3.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.8 5.2 4.1 5.9 6.9 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 144.2422 184.8548 163.3948 171.3258 111.631 108.6535 185.9413 232.1701 207.9186 197.4994 199.948 109.5544 152.0069 200.8201 182.0814 159.5784 168.0825 177.6941
Actin 0.0044 0.0008 0.0254 0.0005 0.0542 0.0057 0.001 0.0005 0.005 0.0004 0.0058 0.004 0.0075 0.0083 0.018 0.0044 0.0015 0.0045
Bud 0.0031 0.0024 0.004 0.0026 0.0031 0.0051 0.003 0.0018 0.003 0.005 0.0056 0.0069 0.0052 0.004 0.0072 0.0077 0.0067 0.007
Bud Neck 0.0016 0.0015 0.0018 0.0007 0.0014 0.0027 0.0009 0.0012 0.0011 0.0008 0.0013 0.0014 0.0011 0.0022 0.0022 0.002 0.0021 0.0016
Bud Periphery 0.0046 0.0018 0.0039 0.0018 0.0024 0.0036 0.0039 0.0012 0.0031 0.0035 0.0063 0.0036 0.0093 0.0029 0.006 0.0089 0.0042 0.0063
Bud Site 0.0041 0.0017 0.0093 0.0005 0.0076 0.0014 0.0007 0.0038 0.0011 0.0008 0.0008 0.0025 0.0023 0.0052 0.0024 0.0057 0.0017 0.0012
Cell Periphery 0.0663 0.0471 0.0665 0.0644 0.0155 0.0333 0.0407 0.0162 0.039 0.0226 0.0226 0.0095 0.0825 0.0496 0.0596 0.0918 0.0458 0.0926
Cytoplasm 0.3375 0.5833 0.3348 0.263 0.2009 0.4297 0.5252 0.7684 0.5753 0.417 0.4886 0.2794 0.4144 0.71 0.5255 0.2402 0.3186 0.4392
Cytoplasmic Foci 0.0129 0.0122 0.0058 0.0024 0.0356 0.0475 0.0032 0.0023 0.0062 0.0027 0.011 0.0996 0.0055 0.0024 0.0042 0.0016 0.0272 0.0025
Eisosomes 0.0076 0.0001 0.0016 0.0001 0.0009 0.0011 0.0004 0.0005 0.0003 0.0001 0.0001 0.0009 0.0016 0.0036 0.0001 0.0014 0.0028 0.0001
Endoplasmic Reticulum 0.0542 0.0191 0.0143 0.0016 0.0018 0.0115 0.0409 0.0192 0.0186 0.0137 0.0222 0.0361 0.0183 0.0066 0.0104 0.0103 0.0053 0.0052
Endosome 0.0131 0.0074 0.0185 0.0123 0.0175 0.0295 0.018 0.0122 0.0166 0.0144 0.0469 0.0734 0.0142 0.0039 0.0239 0.0153 0.0215 0.0051
Golgi 0.0054 0.001 0.01 0.0008 0.0073 0.0126 0.0032 0.0017 0.0029 0.0004 0.0091 0.019 0.0022 0.0022 0.0038 0.0048 0.0027 0.001
Lipid Particles 0.0074 0.002 0.0056 0.0008 0.0127 0.0091 0.0016 0.0005 0.0022 0.0009 0.0042 0.061 0.0041 0.003 0.0024 0.0058 0.0082 0.0086
Mitochondria 0.0312 0.0072 0.0168 0.0016 0.0573 0.0397 0.0134 0.0103 0.0147 0.0055 0.0191 0.0907 0.0339 0.0045 0.0346 0.0169 0.0268 0.0038
None 0.006 0.001 0.0022 0.0002 0.0204 0.0197 0.002 0.0003 0.0016 0.003 0.0014 0.0384 0.0108 0.004 0.0014 0.0016 0.0126 0.0008
Nuclear Periphery 0.0574 0.0189 0.0167 0.0076 0.0135 0.0112 0.0222 0.0041 0.0112 0.006 0.0107 0.0127 0.0361 0.0115 0.0135 0.0368 0.0293 0.0264
Nucleolus 0.0143 0.0021 0.007 0.0028 0.0317 0.0901 0.0046 0.0006 0.0023 0.0041 0.0017 0.0174 0.0138 0.0047 0.004 0.011 0.0404 0.0039
Nucleus 0.0186 0.0077 0.0102 0.0117 0.0181 0.0178 0.009 0.0029 0.0061 0.006 0.0056 0.0129 0.0294 0.01 0.0132 0.0232 0.0189 0.0179
Peroxisomes 0.0041 0.0003 0.0019 0.0001 0.0028 0.0018 0.0002 0.0001 0.0007 0.0002 0.0016 0.0041 0.0006 0.0006 0.0014 0.0006 0.0023 0.0003
Punctate Nuclear 0.0004 0.0001 0.001 0 0.0004 0.0021 0.0001 0 0.0003 0.0001 0.001 0.0054 0.0013 0.0003 0.0002 0.0005 0.0008 0.0002
Vacuole 0.2267 0.2177 0.3159 0.436 0.2571 0.1313 0.22 0.1229 0.2221 0.3518 0.2345 0.121 0.2274 0.136 0.2023 0.3799 0.2718 0.2639
Vacuole Periphery 0.1189 0.0646 0.1268 0.1885 0.2377 0.0935 0.0858 0.0292 0.0665 0.1409 0.1 0.1 0.0787 0.0247 0.0636 0.1295 0.1488 0.108

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1840.3497 1963.1526 2206.4862 1743.0561 2168.5434 1986.9067 1691.0222 1693.0522 1827.5761 1405.6159
Translational Efficiency 2.0463 1.9741 1.7468 2.2142 1.9938 1.9305 1.7884 2.1941 1.7882 2.1175

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Stm1

Stm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available