Standard name
Human Ortholog
Description Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0 0.08 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.97 0.99 0.91 0.91 0.82 0.74 0.83 0.71 0.72 0.99 0.99 0.98 0.95 0.98 0.95 0.96 0.94 0.99 0.97 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.19 0.13 0.25 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.12 0 0 0.07 0.08 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 7 3 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 4 0 0 0 0 0 2 1 0 3 5 0 0 0 0 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 8 5 5 3 10 8 8 15 9 5 14 2 3 0 10 6 2 0 0 0 0 0 0
Cytoplasm 148 300 117 110 132 203 193 222 140 180 115 182 179 343 268 183 233 172 142 291 124 202 288 306
Endoplasmic Reticulum 1 1 0 0 0 11 6 6 10 11 5 12 4 3 0 8 5 4 0 0 0 1 2 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3
Golgi 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 1 0 0 0 0 1
Mitochondria 0 5 0 0 0 2 8 12 35 28 40 43 0 0 0 6 3 3 0 2 0 0 0 0
Nucleus 0 1 1 2 0 1 2 3 2 5 1 1 1 1 1 7 0 2 0 1 0 0 2 1
Nuclear Periphery 0 1 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1 2 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 1 1 1 32 3 5 12 20 0 3 6 1 3 9 1 5 0 0 2 1
Unique Cell Count 150 305 117 113 134 222 212 271 188 216 163 252 180 345 273 192 237 181 149 311 126 208 304 322
Labelled Cell Count 154 321 123 117 136 228 220 293 210 239 185 281 186 353 275 215 251 193 149 311 126 208 304 322


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 11.5 16.4 15.7 14.7 12.0 10.4 11.0 9.4 9.2 8.9 9.3 17.9 15.5 14.9 17.8 21.0 20.7 17.3 17.8 17.3
Std Deviation (1e-4) 3.1 2.6 2.5 2.8 2.2 2.1 1.9 1.8 1.9 1.8 1.8 1.6 2.9 3.3 2.7 4.1 5.2 6.6 3.3 3.7 3.9
Intensity Change (Log2) – – – -0.06 -0.16 -0.46 -0.65 -0.58 -0.81 -0.84 -0.89 -0.82 0.12 -0.08 -0.14 0.12 0.36 0.34 0.07 0.11 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 0 0 1.3 0 0 0.5 0 0 0 0.4 0 0
Cytoplasm -1.8 -1.3 -3.3 -3.3 -4.9 -6.0 -4.7 -6.4 -6.3 -0.8 -0.8 -1.5 -2.4 -1.4 -2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.0 4.1 5.8 4.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.9 0 0 3.0 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.5998 16.8683 15.2438 14.4495 12.8033 15.4682 12.662 16.8355 15.2151 13.1479 12.4177 15.5546 10.679 15.1036 13.8239 11.223 10.0769 12.8112
Actin 0.0381 0.0004 0.0046 0.0009 0.0042 0.0009 0.0173 0.0002 0.0007 0.0975 0.0072 0.0018 0.0101 0.0033 0.0031 0.0244 0.0168 0.0078
Bud 0.0009 0.0003 0.0005 0.0011 0.0005 0.0002 0.0007 0.0002 0.0003 0.0037 0.0005 0.0001 0.0004 0.0009 0.0004 0.0018 0.001 0.0005
Bud Neck 0.0021 0.0002 0.0007 0.0008 0.0006 0.0008 0.0004 0.0001 0.0002 0.0012 0.0011 0.0012 0.0005 0.0003 0.0003 0.0008 0.0027 0.0012
Bud Periphery 0.0009 0.0001 0.0003 0.0006 0.0004 0.0001 0.0006 0 0.0001 0.0029 0.0002 0.0002 0.0003 0.0005 0.0002 0.0008 0.0018 0.0003
Bud Site 0.0036 0.0007 0.001 0.0005 0.0004 0.0002 0.004 0.0003 0.0005 0.0323 0.0004 0 0.0007 0.0033 0.001 0.0025 0.0039 0.0002
Cell Periphery 0.0007 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0004 0.0004 0.0001
Cytoplasm 0.7496 0.9864 0.9062 0.8904 0.8137 0.9105 0.7911 0.994 0.9466 0.7797 0.8048 0.9529 0.8267 0.979 0.9504 0.7701 0.7301 0.9264
Cytoplasmic Foci 0.032 0.0024 0.0098 0.0129 0.0369 0.0122 0.028 0.0012 0.007 0.0124 0.0284 0.0048 0.0228 0.0025 0.0079 0.0188 0.0177 0.0115
Eisosomes 0.0004 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0006 0.0005 0
Endoplasmic Reticulum 0.05 0.0037 0.0112 0.0332 0.0077 0.025 0.037 0.0014 0.0118 0.001 0.0066 0.0224 0.0346 0.0033 0.0054 0.0033 0.0064 0.0182
Endosome 0.0448 0.002 0.0241 0.0361 0.034 0.0257 0.0561 0.0007 0.0188 0.009 0.0591 0.0096 0.0306 0.0013 0.011 0.0227 0.0436 0.0118
Golgi 0.0119 0.0005 0.0056 0.0049 0.0108 0.0102 0.0181 0.0001 0.0012 0.0209 0.0345 0.0015 0.0073 0.0004 0.0031 0.0053 0.0105 0.0036
Lipid Particles 0.0101 0 0.0059 0.0001 0.0324 0.0041 0.0053 0 0.0002 0.0026 0.0227 0.0001 0.0108 0.0006 0.0028 0.0134 0.0301 0.0026
Mitochondria 0.0103 0.0002 0.0041 0.002 0.0213 0.0029 0.0056 0 0.0009 0.0016 0.0193 0.0008 0.0077 0.0001 0.0015 0.0416 0.0711 0.0036
None 0.0032 0.0007 0.0021 0.0005 0.0007 0.0006 0.0038 0.0005 0.0008 0.0009 0.0007 0.0005 0.0139 0.0015 0.0049 0.0307 0.0056 0.0012
Nuclear Periphery 0.0059 0.0003 0.0019 0.0024 0.0034 0.0011 0.0173 0.0002 0.0022 0.0011 0.0008 0.0011 0.0086 0.0004 0.0011 0.0117 0.0101 0.001
Nucleolus 0.0031 0 0.0004 0 0.0001 0 0.0002 0 0 0.0001 0.0001 0 0.0045 0 0.0001 0.0019 0.0063 0.0002
Nucleus 0.0033 0.0006 0.0018 0.0016 0.0009 0.001 0.0028 0.0005 0.0012 0.0018 0.0008 0.0007 0.004 0.0007 0.0009 0.0038 0.0041 0.0012
Peroxisomes 0.017 0 0.0109 0.0001 0.0223 0.0019 0.0031 0 0.0001 0.0047 0.0076 0 0.006 0.0004 0.0019 0.0237 0.0211 0.0051
Punctate Nuclear 0.0035 0 0.0008 0.0001 0.0006 0.0003 0.0016 0 0.0002 0.0012 0.0006 0 0.0037 0.0001 0.0003 0.0124 0.0074 0.0018
Vacuole 0.007 0.001 0.0063 0.0085 0.0063 0.0016 0.0047 0.0004 0.0061 0.0222 0.003 0.0013 0.0055 0.0008 0.0029 0.0072 0.0062 0.0013
Vacuole Periphery 0.0017 0.0001 0.0015 0.003 0.0029 0.0006 0.002 0.0001 0.0009 0.0029 0.0015 0.0004 0.0012 0.0001 0.0007 0.0021 0.0026 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 133.1906 145.2802 111.0464 113.7319 118.0295 79.3816 103.2667 101.0062 95.1374 102.838
Translational Efficiency 1.1749 1.1097 1.4142 1.1278 1.185 1.687 1.2536 1.1471 1.151 1.2085

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1966 557 2954 112 2427 2374 2267 1483 4393 2931 5221 1595

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1264.50 1572.44 1719.97 2284.36 1317.91 1533.59 1830.18 1831.77 1294.01 1540.97 1767.82 1863.55
Standard Deviation 237.18 282.82 259.42 286.76 241.34 244.49 285.52 361.91 240.96 252.68 276.51 375.41
Intensity Change Log 2 — 0.314438 0.443816 0.853223 — 0.218661 0.473734 0.474987 — 0.266354 0.459162 0.672570

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000083 0.000415 0.000065 0.001119 0.000091 0.000208 0.000085 0.000434 0.000087 0.000248 0.000073 0.000482
Bud Neck 0.000496 0.000539 0.000215 0.008183 0.000218 0.000212 0.000770 0.002706 0.000342 0.000274 0.000456 0.003090
Bud Site 0.006700 0.014137 0.003507 0.028015 0.005553 0.007571 0.006255 0.018531 0.006066 0.008819 0.004700 0.019197
Cell Periphery 0.000080 0.000430 0.000167 0.004732 0.000098 0.000111 0.000264 0.000283 0.000090 0.000171 0.000209 0.000595
Cytoplasm 0.842526* 0.916712* 0.965266* 0.691554* 0.924282* 0.930641* 0.878492* 0.767378* 0.887694* 0.927994* 0.927588* 0.762054*
Cytoplasmic Foci 0.055503 0.034503 0.007377 0.027376 0.042834 0.034992 0.011031 0.015323 0.048504 0.034899 0.008963 0.016169
Eisosomes 0.000003 0.000003 0.000001 0.000010 0.000002 0.000002 0.000001 0.000008 0.000002 0.000002 0.000001 0.000008
Endoplasmic Reticulum 0.002681 0.000593 0.000191 0.000350 0.001242 0.000548 0.000457 0.001249 0.001886 0.000557 0.000307 0.001186
Endosome 0.005308 0.001673 0.000456 0.004858 0.001272 0.001663 0.001973 0.008812 0.003078 0.001665 0.001115 0.008534
Golgi 0.004210 0.005252 0.002306 0.016183 0.005005 0.006536 0.003546 0.006469 0.004650 0.006292 0.002845 0.007151
Lipid Particles 0.000664 0.001983 0.001150 0.026293 0.000475 0.000551 0.002050 0.004179 0.000559 0.000823 0.001540 0.005732
Mitochondria 0.000198 0.000074 0.000105 0.000015 0.000261 0.000300 0.000068 0.001321 0.000233 0.000257 0.000089 0.001229
Mitotic Spindle 0.000785 0.004273 0.000669 0.001432 0.000447 0.000819 0.000788 0.014466 0.000598 0.001476 0.000720 0.013551
None 0.001389 0.000811 0.000606 0.000728 0.001531 0.001284 0.000286 0.002495 0.001467 0.001194 0.000467 0.002371
Nuclear Periphery 0.000068 0.000020 0.000011 0.000184 0.000015 0.000007 0.000035 0.000333 0.000039 0.000010 0.000022 0.000323
Nuclear Periphery Foci 0.001014 0.001747 0.000674 0.011868 0.000306 0.000427 0.001288 0.002599 0.000623 0.000678 0.000940 0.003250
Nucleolus 0.000101 0.000032 0.000037 0.000114 0.000069 0.000052 0.000118 0.000736 0.000083 0.000049 0.000072 0.000692
Nucleus 0.009063 0.004887 0.004983 0.015237 0.004289 0.003607 0.008325 0.025853 0.006426 0.003850 0.006434 0.025108
Peroxisomes 0.000193 0.000226 0.000082 0.000406 0.000187 0.000213 0.000116 0.000997 0.000190 0.000215 0.000096 0.000956
Vacuole 0.061830 0.010788 0.011042 0.158814 0.007735 0.007814 0.078701 0.118363 0.031944 0.008379 0.040420 0.121203
Vacuole Periphery 0.007105 0.000904 0.001091 0.002529 0.004088 0.002442 0.005353 0.007464 0.005438 0.002150 0.002941 0.007117

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.23 1.25 -4.70 -3.11 -4.75 -2.30 -0.39 -4.15 -1.55 -4.20 -3.77 0.38 -5.61 -1.98 -5.78
Bud Neck -1.14 5.32 -6.85 -6.78 -7.03 0.16 -10.57 -13.35 -13.33 -9.45 1.47 -5.60 -14.28 -14.48 -13.26
Bud Site -5.43 5.07 -5.29 -3.10 -6.14 -2.71 -2.06 -5.75 -3.88 -4.87 -4.14 3.09 -6.34 -3.87 -7.30
Cell Periphery -7.81 -7.84 -4.85 -4.27 -4.71 -1.59 -6.19 -6.88 -6.08 1.51 -7.72 -9.10 -6.54 -4.78 -4.07
Cytoplasm -9.77 -24.54 9.32 11.97 14.10 -1.67 13.25 28.62 29.23 19.56 -11.05 -10.44 25.26 31.09 31.04
Cytoplasmic Foci 5.75 21.92 4.37 -0.41 -8.00 3.33 17.62 15.14 11.91 -2.91 6.94 28.25 20.81 12.15 -7.43
Eisosomes -0.13 9.97 -5.61 -5.54 -6.70 -0.73 2.26 -4.47 -4.19 -5.63 -0.37 6.29 -5.11 -4.92 -6.81
Endoplasmic Reticulum 3.91 5.22 5.02 1.21 -1.46 2.82 3.34 1.45 -0.42 -0.80 5.09 6.32 3.25 -0.33 -1.24
Endosome 7.28 11.33 2.05 -3.07 -5.22 -1.84 -3.64 -5.13 -4.48 -4.07 5.47 9.03 -2.67 -4.70 -5.57
Golgi -1.28 3.63 -1.29 -0.97 -1.89 -2.36 4.47 2.71 3.85 -0.04 -2.92 6.27 1.53 3.39 -1.53
Lipid Particles -3.55 -3.45 -6.82 -6.40 -6.66 -1.08 -6.70 -5.38 -5.22 -2.15 -2.91 -7.55 -7.43 -6.92 -5.49
Mitochondria 2.37 1.59 4.17 1.63 2.08 -0.41 3.83 -1.86 -1.59 -2.79 -0.34 3.56 -1.94 -1.73 -2.66
Mitotic Spindle -1.68 0.20 -1.34 -0.69 -1.35 -1.12 -2.75 -5.28 -5.01 -4.83 -1.78 -1.66 -5.37 -4.75 -5.18
None 1.55 2.24 1.54 0.18 -0.78 0.93 4.86 -0.48 -0.91 -2.48 1.20 4.77 -0.52 -1.00 -2.24
Nuclear Periphery 3.80 4.99 -4.98 -5.85 -6.01 1.26 -5.21 -4.77 -4.90 -4.28 4.75 1.59 -4.77 -5.24 -4.94
Nuclear Periphery Foci -2.92 2.54 -6.02 -5.61 -6.18 -2.11 -11.60 -9.09 -8.46 -4.49 -0.76 -5.48 -9.53 -9.23 -8.18
Nucleolus 6.64 9.42 -0.90 -5.09 -5.72 0.45 -1.82 -3.15 -3.31 -2.82 1.54 0.41 -3.11 -3.37 -3.19
Nucleus 3.22 8.03 -4.62 -5.38 -5.59 1.58 -10.07 -20.79 -21.60 -16.36 6.56 -3.81 -20.36 -21.94 -19.48
Peroxisomes -1.27 6.29 -1.76 -1.24 -3.27 -0.36 1.01 -2.50 -2.50 -3.22 -0.58 2.39 -2.70 -2.55 -3.47
Vacuole 14.64 16.46 -8.06 -10.46 -10.47 -0.09 -27.15 -30.55 -30.49 -14.52 15.25 -6.22 -26.93 -32.05 -24.72
Vacuole Periphery 10.28 10.29 8.14 -0.94 -0.54 2.41 -1.06 0.31 -2.22 1.49 6.43 5.98 2.78 -2.69 -1.85
Standard name
Human Ortholog
Description Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Dph5

Dph5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dph5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available