Standard name
Human Ortholog
Description Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.99 0.92 0.85 0.74 – 0.66 0.59 0.61 0.59 0.99 0.98 0.98 0.96 0.98 0.98 0.88 0.92 0.93 0.87 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0 – 0.14 0.11 0.15 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.08 0.26 – 0.24 0.26 0.27 0.19 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 0 – 1 1 2 7 0 1 0 0 0 0 3 1 2 0 2 5
Bud 1 0 0 0 0 3 6 – 7 9 9 17 1 0 0 0 0 0 3 0 1 2 10 5
Bud Neck 1 1 9 4 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 3
Bud Site 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 16 5 3 3 4 – 5 6 13 6 0 1 2 5 5 4 1 0 1 2 1 1
Cytoplasm 308 279 315 307 416 249 346 – 333 253 291 255 265 433 425 110 108 113 288 268 303 206 331 286
Endoplasmic Reticulum 0 1 1 0 0 0 2 – 0 9 3 9 2 0 2 5 7 8 0 0 0 2 2 3
Endosome 0 0 1 0 1 0 0 – 1 0 2 0 0 0 0 0 1 0 2 2 0 1 1 3
Golgi 1 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 4 1 0 2 2 2
Mitochondria 6 6 0 4 4 42 19 – 70 46 72 82 1 0 0 2 1 0 10 6 7 8 11 5
Nucleus 0 0 0 1 6 6 14 – 12 17 14 23 1 1 1 2 2 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 1 0 1 – 5 4 1 7 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 0 0 1 2 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 0 1 0 2 0 2 0 0 0 0 3 1 1 2 5 5
Vac/Vac Membrane 0 0 0 2 38 24 120 – 118 112 128 81 0 5 7 2 3 1 2 3 0 1 0 3
Unique Cell Count 315 282 317 311 452 294 470 502 428 477 429 269 441 433 115 110 115 327 291 327 237 376 332
Labelled Cell Count 321 288 342 324 471 328 512 553 458 537 491 270 444 439 126 127 126 327 291 327 237 376 332


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.2 4.7 5.0 7.0 5.8 8.2 – 10.2 10.0 11.5 10.9 5.8 8.6 10.7 8.5 9.2 8.7 4.9 5.4 5.6
Std Deviation (1e-4) 0.9 0.8 0.8 1.6 2.1 2.0 2.7 – 5.5 5.1 7.0 6.6 2.4 5.6 6.5 1.7 1.9 1.7 7.4 7.3 7.7
Intensity Change (Log2) – – – 0.07 0.57 0.31 0.79 – 1.11 1.09 1.28 1.21 0.31 0.87 1.18 0.84 0.97 0.88 0.05 0.18 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.426 1.003 0.8534 0.8139 0.2445 0.9029 2.638 2.7679 2.9676 2.8168 1.6662 1.9853 6.3879 6.3266 6.1752 6.2504 5.0574 5.6835
Actin 0.0062 0.0008 0.0015 0.0398 0.0002 0.0031 0.024 0.0041 0.0229 0.0619 0.0569 0.0088 0.0003 0.0157 0.0015 0.0075 0.0029 0.0004
Bud 0.0044 0.0037 0.0072 0.0128 0.0001 0.0004 0.0023 0.0034 0.0003 0.0007 0.0005 0.0002 0 0.0048 0.0006 0.0009 0.0113 0.0002
Bud Neck 0.0009 0.0003 0.0026 0.0009 0.0003 0.0017 0.0033 0.0005 0.0009 0.0032 0.0016 0.0013 0.0001 0.0007 0.0005 0.0026 0.0005 0.0008
Bud Periphery 0.0028 0.0008 0.0027 0.0071 0 0.0002 0.0031 0.0012 0.0006 0.0005 0.0005 0.0003 0 0.0038 0.0003 0.0009 0.0052 0.0002
Bud Site 0.0006 0.0048 0.0066 0.0017 0.0001 0.0003 0.004 0.0047 0.0007 0.0023 0.0006 0.0003 0.0001 0.0073 0.0009 0.0022 0.0008 0.0001
Cell Periphery 0.0002 0.0002 0.0003 0.0001 0 0 0.0003 0.0074 0.0001 0.0002 0.0001 0 0 0.0003 0.0001 0.0003 0.0002 0
Cytoplasm 0.6907 0.7926 0.7305 0.6658 0.6924 0.765 0.5779 0.6885 0.6455 0.6355 0.5249 0.6682 0.8126 0.8564 0.8211 0.7778 0.7131 0.8125
Cytoplasmic Foci 0.0143 0.0106 0.0159 0.0399 0.0137 0.0143 0.0214 0.0084 0.0084 0.0042 0.0102 0.0068 0.0029 0.004 0.0089 0.0045 0.0083 0.0064
Eisosomes 0.0001 0 0 0.0001 0 0 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0043 0.0028 0.0037 0.0022 0.0025 0.0023 0.0032 0.0018 0.0022 0.0044 0.0014 0.0021 0.0027 0.0019 0.0039 0.0033 0.0052 0.0021
Endosome 0.0137 0.0047 0.0083 0.018 0.0059 0.006 0.0215 0.001 0.0048 0.0024 0.0049 0.0036 0.0016 0.0011 0.0038 0.0021 0.0022 0.0031
Golgi 0.0011 0.0002 0.0003 0.0009 0.0001 0.0003 0.0036 0.0004 0.0039 0.0006 0.0054 0.0031 0.0001 0.0002 0.0006 0.0003 0.0003 0.0001
Lipid Particles 0.0022 0.0002 0.0003 0.0085 0.0001 0.0002 0.005 0.0002 0.0006 0.0001 0.0009 0.0059 0.0001 0 0.0012 0.0003 0.0002 0.0001
Mitochondria 0.0021 0.0004 0.0012 0.0006 0.0002 0.0003 0.0015 0.0005 0.0008 0.0007 0.001 0.0035 0.0001 0.0002 0.0004 0.0003 0.0013 0.0001
None 0.2401 0.1644 0.1997 0.1773 0.2708 0.19 0.2837 0.2721 0.3008 0.272 0.385 0.2835 0.1687 0.097 0.1482 0.1904 0.2393 0.1672
Nuclear Periphery 0.0039 0.0018 0.0023 0.0019 0.002 0.0013 0.0119 0.0008 0.0008 0.0029 0.0009 0.0007 0.0019 0.0007 0.0012 0.001 0.0015 0.0007
Nucleolus 0.0009 0.0002 0.0009 0.0002 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0003 0.0001
Nucleus 0.004 0.0054 0.0081 0.0135 0.0072 0.0085 0.021 0.0022 0.002 0.0047 0.0013 0.0014 0.0074 0.0044 0.0032 0.0028 0.004 0.004
Peroxisomes 0.0014 0.0002 0.0003 0.0025 0.0001 0.0002 0.0034 0.0004 0.0026 0.0005 0.0005 0.009 0 0.0002 0.0004 0.0001 0.0003 0
Punctate Nuclear 0.0035 0.0032 0.0046 0.0035 0.0019 0.0045 0.0039 0.001 0.001 0.0019 0.0009 0.0006 0.0004 0.0007 0.002 0.0013 0.0017 0.0012
Vacuole 0.002 0.0024 0.0025 0.0022 0.0023 0.0009 0.0035 0.0013 0.0008 0.001 0.002 0.0006 0.0006 0.0004 0.001 0.0008 0.001 0.0006
Vacuole Periphery 0.0005 0.0003 0.0004 0.0004 0.0003 0.0002 0.0009 0.0001 0.0001 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.1083 11.9561 7.7629 25.4587 22.7597 41.7725 23.0442 26.5171 46.3165 28.5313
Translational Efficiency 1.092 0.9492 1.8019 1.5156 1.1018 1.8513 1.2732 1.5992 1.4724 1.2674

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
692 2139 1119 1071 1949 1240 2172 2326 2641 3379 3291 3397

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 622.06 709.10 987.85 684.47 592.98 684.23 802.81 698.09 600.60 699.97 865.73 693.80
Standard Deviation 94.91 100.58 242.05 88.78 158.61 187.29 119.09 87.50 145.22 139.35 192.26 88.13
Intensity Change Log 2 — 0.188935 0.667238 0.137934 — 0.206498 0.437075 0.235430 — 0.197533 0.559488 0.186338

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.000067 0.000258 0.000099 0.000081 0.000880 0.000083 0.000061 0.000105 0.000365 0.000142 0.000073
Bud Neck 0.020405 0.007250 0.002461 0.005568 0.010261 0.050599 0.002345 0.006729 0.012919 0.023158 0.002384 0.006363
Bud Site 0.010876 0.003744 0.008652 0.013210 0.004504 0.017424 0.008443 0.007797 0.006174 0.008764 0.008514 0.009504
Cell Periphery 0.000498 0.000057 0.000136 0.000167 0.000187 0.000227 0.000108 0.000097 0.000268 0.000119 0.000118 0.000119
Cytoplasm 0.515872* 0.643318* 0.640424* 0.553396* 0.508914* 0.368444* 0.649791* 0.654083* 0.510737* 0.542447* 0.646606* 0.622339*
Cytoplasmic Foci 0.256173* 0.082109 0.009965 0.024706 0.096755 0.318908* 0.019402 0.041934 0.138526 0.169008 0.016193 0.036502
Eisosomes 0.000353 0.000035 0.000018 0.000052 0.000085 0.000344 0.000020 0.000025 0.000155 0.000148 0.000019 0.000033
Endoplasmic Reticulum 0.001199 0.000373 0.001476 0.001175 0.000646 0.000507 0.000806 0.000876 0.000791 0.000422 0.001034 0.000970
Endosome 0.008177 0.004405 0.001521 0.002836 0.002752 0.022464 0.001800 0.004535 0.004173 0.011032 0.001705 0.003999
Golgi 0.005325 0.000474 0.000160 0.000194 0.000986 0.014010 0.000204 0.000172 0.002123 0.005441 0.000189 0.000179
Lipid Particles 0.010590 0.001617 0.000686 0.001340 0.004104 0.009677 0.000598 0.001427 0.005803 0.004575 0.000628 0.001399
Mitochondria 0.021463 0.002010 0.000462 0.001240 0.004214 0.024036 0.000240 0.000664 0.008734 0.010093 0.000315 0.000846
Mitotic Spindle 0.000342 0.002779 0.005403 0.002896 0.001499 0.004578 0.002477 0.001355 0.001196 0.003439 0.003472 0.001841
None 0.008092 0.003878 0.008966 0.006889 0.009270 0.004386 0.006663 0.004137 0.008961 0.004064 0.007446 0.005005
Nuclear Periphery 0.000248 0.000503 0.000577 0.000975 0.000558 0.000283 0.000561 0.000805 0.000476 0.000422 0.000567 0.000859
Nuclear Periphery Foci 0.000413 0.000215 0.001822 0.000378 0.000326 0.000237 0.001025 0.000634 0.000349 0.000223 0.001296 0.000553
Nucleolus 0.000919 0.000708 0.000294 0.000548 0.001400 0.000870 0.000352 0.000600 0.001274 0.000767 0.000332 0.000584
Nucleus 0.106144 0.197828* 0.280865* 0.304032* 0.331334* 0.090712 0.274969* 0.222774* 0.272329* 0.158519* 0.276974* 0.248393*
Peroxisomes 0.005908 0.002021 0.000144 0.000343 0.002453 0.023985 0.000251 0.000684 0.003358 0.010081 0.000214 0.000577
Vacuole 0.023845 0.045494 0.035392 0.079575 0.019158 0.045208 0.029674 0.050126 0.020386 0.045389 0.031618 0.059410
Vacuole Periphery 0.002986 0.001117 0.000318 0.000381 0.000516 0.002218 0.000186 0.000483 0.001163 0.001521 0.000231 0.000451

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.22 -5.52 3.30 -2.51 9.07 -2.00 -0.47 0.84 2.05 3.17 -1.77 -3.61 1.72 1.99 9.20
Bud Neck 6.26 8.85 7.20 1.97 -2.03 -12.97 6.92 2.56 14.37 -4.38 -6.58 10.52 5.84 12.08 -4.82
Bud Site 4.85 1.32 0.53 -2.83 -0.57 -7.03 -2.30 -2.50 4.84 0.16 -2.63 -1.53 -1.82 0.32 -0.19
Cell Periphery 10.23 7.90 8.08 -9.36 0.65 -1.99 6.10 6.99 6.55 0.82 9.89 9.72 10.44 0.79 1.13
Cytoplasm -10.95 -6.13 1.44 13.76 8.15 14.06 -15.06 -17.92 -30.31 -2.48 -4.06 -15.76 -13.27 -9.26 2.58
Cytoplasmic Foci 21.11 31.64 29.60 19.89 -8.19 -29.68* 25.11 16.78 39.36 -12.19 -6.35 36.71 29.78 35.35 -14.52
Eisosomes 8.55 8.89 7.95 -7.54 -11.80 -9.14 15.25 14.71 11.39 -1.62 0.48 12.71 11.50 10.63 -9.18
Endoplasmic Reticulum 5.28 -3.45 -0.47 -7.00 3.28 1.32 -2.81 -2.46 -4.34 0.53 4.51 -4.92 -2.48 -9.27 2.94
Endosome 4.42 8.31 6.29 3.19 -1.54 -14.49 2.41 -4.17 13.13 -6.71 -10.85 6.91 0.70 11.56 -6.66
Golgi 4.42 4.69 4.71 2.56 0.44 -8.60 2.51 2.62 9.34 0.27 -4.94 5.21 5.29 9.44 0.46
Lipid Particles 10.57 11.53 10.88 1.53 -4.40 -5.85 13.13 9.83 9.12 -8.21 2.81 16.99 14.45 9.02 -9.21
Mitochondria 6.47 7.05 6.86 1.78 -1.64 -7.71 4.94 4.39 9.56 -4.56 -0.94 8.49 8.00 9.59 -3.82
Mitotic Spindle -2.72 -4.35 -2.71 -0.48 1.56 -2.52 -1.12 0.28 2.82 1.46 -2.72 -3.26 -1.17 1.73 2.23
None 3.67 0.23 1.84 -1.86 2.42 3.00 2.48 4.08 0.26 3.45 4.71 2.31 4.38 -0.75 4.31
Nuclear Periphery -3.10 -13.43 -18.00 -8.09 -8.78 3.03 -1.15 -3.76 -7.42 -4.45 0.63 -4.01 -9.19 -8.56 -8.52
Nuclear Periphery Foci 2.36 -9.66 -0.51 -5.45 9.95 0.97 -6.03 -3.06 -5.52 4.15 1.74 -10.46 -3.01 -7.55 9.35
Nucleolus 1.81 7.65 3.77 1.78 -3.15 1.91 3.71 2.86 3.03 -4.23 2.38 4.43 3.31 2.87 -5.30
Nucleus -13.63 -21.63 -22.85 -14.45 -2.67 34.54* 6.25 14.67 -23.21 8.82 19.47 -2.57 2.37 -19.48 5.50
Peroxisomes 5.65 11.30 11.00 3.77 -2.41 -12.69 5.62 4.25 14.07 -2.58 -8.81 9.74 8.28 13.56 -2.93
Vacuole -8.45 -8.69 -16.69 -12.45 -8.77 -10.17 -9.23 -14.26 -2.56 -4.91 -14.56 -12.86 -21.61 -9.81 -9.33
Vacuole Periphery 3.07 5.52 5.56 1.98 -0.05 -7.49 3.72 0.37 7.93 -4.35 -1.13 6.46 5.02 4.07 -3.51
Standard name
Human Ortholog
Description Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (30%), nucleus (13%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tfs1

Tfs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available