ORF
Human Ortholog
Description Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.91 0.83 0.84 0.8 – 0.84 0.78 0.75 0.8 1.0 1.0 0.99 0.99 0.99 0.99 0.96 0.95 0.98 0.95 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.05 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0.19 0.12 0.2 – 0.06 0.09 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 3 4 3 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 – 1 1 1 1 0 0 0 0 0 1 2 0 0 3 1 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 2 4 1 4 – 4 13 10 9 2 1 6 4 3 1 0 0 0 0 0 0
Cytoplasm 378 281 139 153 108 102 167 – 139 161 126 233 284 466 463 520 364 354 357 262 142 188 364 378
Endoplasmic Reticulum 1 0 0 2 2 0 2 – 6 10 11 21 0 1 2 13 13 9 0 0 0 0 2 1
Endosome 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0
Golgi 0 0 0 0 1 1 2 – 0 1 3 1 0 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 0 2 0 0 0 3 0 – 4 7 7 10 0 0 1 1 1 0 0 0 0 1 1 1
Nucleus 3 0 0 0 0 2 1 – 5 9 3 8 0 1 1 0 0 1 1 4 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 3 2 17 25 15 41 – 10 18 20 25 0 0 0 3 1 2 0 3 0 1 2 2
Unique Cell Count 381 285 141 169 130 121 208 166 207 168 293 284 467 467 523 367 357 373 277 146 199 376 393
Labelled Cell Count 386 287 142 174 141 125 217 172 224 184 312 286 469 473 542 383 368 373 277 146 199 376 393


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.1 31.1 29.2 26.7 27.3 17.1 20.9 – 17.0 16.8 15.6 15.6 37.8 34.8 37.5 69.6 74.1 67.4 28.2 31.3 32.8
Std Deviation (1e-4) 7.0 6.2 4.9 5.3 5.9 8.9 6.5 – 3.5 4.0 3.3 3.2 7.0 7.7 8.1 17.0 19.0 19.7 7.4 6.6 7.8
Intensity Change (Log2) – – – -0.13 -0.1 -0.77 -0.48 – -0.78 -0.79 -0.9 -0.91 0.37 0.26 0.36 1.25 1.34 1.21 -0.05 0.1 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 2.6 2.5 0 0 0 0 0 0 0
Cytoplasm -3.0 -4.5 -4.2 -5.1 – -4.4 -5.5 -5.9 -5.3 2.0 1.8 0.6 1.0 0.6 0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 3.1 3.3 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 4.9 3.6 5.1 – 2.1 2.9 3.6 2.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.453 22.8831 22.0687 21.1673 19.1248 20.7572 26.7639 27.0124 28.2427 25.211 25.5326 27.3133 30.1578 34.0812 30.4996 30.1509 30.7597 31.7279
Actin 0.006 0.0004 0.0006 0.0002 0.001 0.0001 0.001 0.0009 0.0003 0.0001 0.0001 0.0019 0.0026 0.0005 0.0074 0.0001 0.0011 0.0001
Bud 0.0002 0.0006 0.0001 0 0.0001 0.0001 0.0001 0.0022 0.0002 0 0 0.0001 0.0005 0.0002 0.0003 0 0.0001 0
Bud Neck 0.0004 0.0001 0.0009 0.0003 0.0003 0.0007 0.0003 0.0003 0.0003 0.0004 0.0004 0.0011 0.0003 0.0001 0.0003 0.0002 0.0003 0.0006
Bud Periphery 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.001 0.0001 0 0 0.0001 0.0004 0 0.0001 0 0 0
Bud Site 0.0003 0.0003 0.0008 0 0.0003 0.0001 0.0004 0.0044 0.0002 0 0 0.0001 0.0004 0.0001 0.0002 0 0 0
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.8936 0.9865 0.958 0.9706 0.8445 0.943 0.9275 0.9559 0.9519 0.9665 0.9565 0.9277 0.9487 0.9875 0.9724 0.9835 0.9535 0.9738
Cytoplasmic Foci 0.013 0.0008 0.0014 0.0005 0.0118 0.0016 0.0035 0.0013 0.0021 0.0005 0.0003 0.0017 0.0044 0.0003 0.0005 0.0002 0.0007 0.0005
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0042 0.0004 0.0017 0.0024 0.0027 0.0015 0.0014 0.0017 0.0006 0.0006 0.0012 0.0016 0.0021 0.0003 0.0005 0.0002 0.0028 0.0005
Endosome 0.0046 0.0001 0.0032 0.0011 0.0334 0.0006 0.0031 0.0006 0.0008 0.0004 0.0002 0.0013 0.0015 0.0001 0.0003 0.0001 0.0032 0.0001
Golgi 0.0017 0 0.0006 0 0.0047 0 0.0005 0.0002 0.0002 0 0 0.0003 0.0005 0 0.0001 0 0.0001 0
Lipid Particles 0.0012 0 0.0013 0.0001 0.0226 0 0.0008 0.0001 0.003 0 0 0.0002 0.0073 0 0.0001 0 0.0001 0
Mitochondria 0.0007 0.0001 0.0006 0 0.0037 0.0004 0.0012 0.0002 0.0001 0.0001 0 0.0003 0.0021 0.0002 0.0001 0 0.0002 0.0001
None 0.0126 0.0008 0.0013 0.0003 0.0008 0.0008 0.0019 0.0025 0.004 0.0002 0.0003 0.0055 0.001 0.0005 0.0003 0.0002 0.0041 0.0003
Nuclear Periphery 0.011 0.0011 0.0029 0.0049 0.0051 0.0054 0.0059 0.0028 0.0024 0.0028 0.0061 0.0078 0.0044 0.001 0.0019 0.0012 0.0145 0.0025
Nucleolus 0.001 0 0.0003 0 0.0005 0.0029 0.0002 0.0004 0.0001 0 0 0.0001 0.0003 0.0001 0 0 0.0001 0
Nucleus 0.034 0.008 0.0223 0.0176 0.0302 0.0404 0.0484 0.0219 0.0262 0.0277 0.0337 0.0479 0.0202 0.0087 0.0144 0.0139 0.0173 0.0208
Peroxisomes 0.0016 0 0.001 0 0.0016 0 0.0003 0.0001 0.0005 0 0 0.0001 0.001 0 0.0001 0 0 0
Punctate Nuclear 0.0115 0.0004 0.0014 0.0003 0.0022 0.0016 0.002 0.002 0.0066 0.0003 0.0005 0.0014 0.0011 0.0002 0.0005 0.0001 0.0012 0.0005
Vacuole 0.0019 0.0002 0.0011 0.0014 0.0217 0.0007 0.0011 0.0012 0.0003 0.0002 0.0005 0.0006 0.0008 0.0001 0.0004 0.0002 0.0005 0.0002
Vacuole Periphery 0.0004 0 0.0003 0.0001 0.0126 0.0002 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 280.4031 255.5463 231.8998 255.6798 252.5998 245.6718 212.3016 231.5955 249.3195 249.2672
Translational Efficiency 1.453 1.5291 1.5623 1.8306 1.5559 1.5274 1.5263 1.652 1.3395 1.3238

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1861 878 1326 2525 1788 2383 1304 894 3649 3261 2630 3419

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1881.93 2100.38 2459.13 2572.64 2113.52 2349.99 2970.75 2655.98 1995.41 2282.78 2712.80 2594.43
Standard Deviation 424.88 473.14 451.07 698.83 458.32 479.90 585.86 570.16 456.51 490.74 581.55 668.59
Intensity Change Log 2 — 0.158437 0.385935 0.451037 — 0.153007 0.491179 0.329597 — 0.155567 0.442562 0.388072

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000091 0.000189 0.000093 0.000092 0.000097 0.000158 0.000119 0.000583 0.000094 0.000167 0.000106 0.000220
Bud Neck 0.000390 0.000547 0.000421 0.001471 0.000207 0.000669 0.001074 0.006709 0.000300 0.000636 0.000745 0.002841
Bud Site 0.001610 0.002024 0.001099 0.005885 0.000537 0.003206 0.001101 0.020392 0.001084 0.002888 0.001100 0.009678
Cell Periphery 0.000106 0.000217 0.000109 0.000102 0.000089 0.000066 0.000253 0.000293 0.000098 0.000107 0.000180 0.000152
Cytoplasm 0.850939* 0.756739* 0.632938* 0.813951* 0.765709* 0.775568* 0.573580* 0.609578* 0.809176* 0.770499* 0.603507* 0.760512*
Cytoplasmic Foci 0.006401 0.006376 0.002581 0.003223 0.002721 0.004681 0.004155 0.015185 0.004598 0.005138 0.003361 0.006350
Eisosomes 0.000001 0.000002 0.000001 0.000001 0.000001 0.000001 0.000001 0.000007 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.000087 0.000097 0.000265 0.000033 0.000072 0.000078 0.000031 0.000504 0.000080 0.000083 0.000149 0.000156
Endosome 0.000084 0.000343 0.000416 0.000488 0.000055 0.000526 0.000051 0.012732 0.000070 0.000476 0.000235 0.003690
Golgi 0.000513 0.001508 0.000389 0.000527 0.000405 0.000962 0.000187 0.022866 0.000460 0.001109 0.000289 0.006368
Lipid Particles 0.000184 0.000434 0.000159 0.000235 0.000459 0.000256 0.000315 0.001572 0.000319 0.000304 0.000236 0.000585
Mitochondria 0.000111 0.000128 0.000135 0.000228 0.000189 0.000122 0.000148 0.004090 0.000149 0.000124 0.000142 0.001237
Mitotic Spindle 0.001161 0.000907 0.001403 0.008303 0.000462 0.002868 0.001582 0.063920 0.000819 0.002340 0.001492 0.022846
None 0.011235 0.015688 0.012656 0.008647 0.014631 0.021020 0.019017 0.011249 0.012899 0.019585 0.015810 0.009327
Nuclear Periphery 0.000037 0.000062 0.000087 0.000096 0.000050 0.000077 0.000142 0.000718 0.000043 0.000073 0.000114 0.000259
Nuclear Periphery Foci 0.001088 0.001697 0.000372 0.000514 0.001026 0.001022 0.000786 0.005556 0.001058 0.001204 0.000577 0.001833
Nucleolus 0.000078 0.000162 0.000060 0.000155 0.000050 0.000125 0.000075 0.000264 0.000064 0.000135 0.000068 0.000184
Nucleus 0.121995 0.207549* 0.343027* 0.141011* 0.210688* 0.184629* 0.392868* 0.164094* 0.165455* 0.190800* 0.367739* 0.147047*
Peroxisomes 0.000023 0.000370 0.000032 0.000115 0.000016 0.000167 0.000006 0.000864 0.000019 0.000222 0.000019 0.000311
Vacuole 0.003106 0.004603 0.003614 0.014332 0.001762 0.003400 0.004471 0.048334 0.002447 0.003724 0.004039 0.023223
Vacuole Periphery 0.000761 0.000357 0.000145 0.000591 0.000775 0.000398 0.000037 0.010488 0.000768 0.000387 0.000091 0.003179

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.35 -1.16 -0.23 3.29 1.00 -1.58 -3.43 -3.77 -2.61 -1.94 -2.53 -3.62 -3.32 0.11 -0.12
Bud Neck -3.07 -3.94 -7.26 -3.23 -3.25 -5.08 -7.04 -11.88 -10.21 -5.64 -5.15 -7.98 -13.90 -9.53 -3.92
Bud Site -1.05 1.08 -3.00 -1.71 -3.55 -4.52 -4.00 -7.19 -4.93 -5.89 -3.77 -1.65 -7.52 -3.65 -6.23
Cell Periphery -4.43 -3.28 -0.48 4.27 3.03 1.63 -11.88 -10.23 -11.01 1.79 -1.79 -11.72 -6.97 -4.75 6.39
Cytoplasm 15.66 37.34 17.37 -2.69 -22.60 0.03 33.73 16.88 17.39 -7.81 12.27 49.06 18.28 7.19 -27.79
Cytoplasmic Foci -1.34 1.96 2.98 3.96 1.06 -3.49 -8.47 -12.58 -10.56 -5.46 -1.64 -4.64 -5.77 -3.90 -0.55
Eisosomes -1.66 -2.44 -10.87 -0.20 -6.44 -2.01 -6.28 -11.45 -11.49 -7.07 -2.69 -6.74 -13.65 -7.33 -6.69
Endoplasmic Reticulum -0.43 -0.64 5.17 3.28 1.06 -0.39 1.27 -2.32 -2.10 -3.28 -0.31 -0.48 1.36 1.49 0.73
Endosome -1.82 -0.92 -1.78 0.57 0.10 -3.22 -1.04 -4.20 -3.33 -4.15 -3.59 -1.02 -4.58 -1.89 -3.22
Golgi -1.60 1.18 2.32 2.06 0.46 -1.68 1.50 -2.32 -1.97 -2.43 -2.23 1.82 -1.79 -0.29 -2.23
Lipid Particles -1.78 -3.64 -5.25 -0.32 -1.08 0.38 -1.14 -5.49 -11.10 -8.45 -0.30 -1.96 -4.37 -7.12 -4.93
Mitochondria -0.59 -3.54 -2.76 -1.31 -0.82 0.74 0.09 -1.36 -1.55 -1.41 0.53 -0.47 -1.53 -1.86 -1.46
Mitotic Spindle 0.00 -1.20 -5.12 -4.68 -3.57 -4.10 -1.56 -4.56 -3.66 -4.13 -3.37 -1.91 -7.06 -5.16 -5.69
None -2.61 -0.02 5.07 6.24 7.01 -4.33 -0.05 8.61 12.62 8.31 -6.12 -0.08 9.92 14.63 10.87
Nuclear Periphery -5.98 -11.10 -15.65 -10.81 -2.13 -3.75 -15.12 -11.60 -10.46 -6.06 -5.82 -18.66 -17.64 -13.37 -4.29
Nuclear Periphery Foci -3.30 4.52 3.12 5.08 -0.67 -0.20 -1.05 -5.02 -5.05 -4.62 -1.46 1.08 -1.54 -0.07 -2.63
Nucleolus -1.25 -0.31 -6.36 -0.10 -6.63 -4.78 -8.41 -9.30 -3.25 -6.13 -3.50 -5.14 -10.80 -1.61 -8.32
Nucleus -14.87 -37.23 -9.14 8.34 30.23 3.51 -29.57 5.50 3.03 31.10 -8.91 -46.16 0.52 9.18 45.76
Peroxisomes -1.01 -0.47 -1.50 0.90 -0.63 -2.10 0.27 -1.89 -0.38 -1.93 -1.87 -0.33 -2.45 1.08 -2.14
Vacuole -3.78 -6.68 -23.72 -18.59 -16.56 -6.22 -10.74 -20.99 -20.24 -17.99 -6.70 -12.51 -30.03 -27.60 -22.83
Vacuole Periphery 1.04 1.78 1.36 0.44 -0.79 0.70 1.30 -1.14 -2.05 -2.57 1.17 2.08 0.35 -1.16 -2.68
ORF
Human Ortholog
Description Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YLR179C

YLR179C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr179c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available