Standard name
Human Ortholog
Description S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.07 0.08 0.07 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.97 0.99 0.98 0.91 0.86 0.84 0.87 0.9 0.77 1.0 0.99 0.99 0.99 0.99 0.98 0.96 0.96 0.96 0.97 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.12 0.11 0.1 0.06 0.14 0 0 0 0.07 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 4 2 9 6 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 2 3 1 3 3 6 0 0 1 0 0 0 0 0 0 0 7 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 6 1 1 5 24 47 28 36 23 44 3 8 5 1 1 1 0 0 0 0 0 0
Cytoplasm 273 140 230 242 345 379 556 540 287 452 338 342 446 526 631 254 275 187 237 136 207 267 493 597
Endoplasmic Reticulum 1 0 0 1 1 6 44 74 38 51 22 62 0 0 1 17 19 11 0 0 1 0 2 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 1 0 0 0 0 0 0 0 1 0 0 2 0 0 3 1 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 0 0 1 1 1 0 0 1 0 0 0 0 0 2 0 0 1
Nucleus 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 2 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 8 0 1 2 0 1 1 0 11 0 4 3 0 5 1 0 1 0 1 7 3
Unique Cell Count 275 141 230 250 347 387 611 630 343 518 375 446 446 530 636 257 279 190 248 141 216 275 528 626
Labelled Cell Count 275 145 236 252 347 393 630 667 361 546 396 475 449 538 645 275 303 201 248 141 216 275 528 626


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 133.9 178.6 146.7 128.3 121.6 95.4 76.1 76.1 64.7 61.4 55.6 53.2 172.2 177.2 168.0 216.4 218.6 200.4 149.7 156.6 175.6
Std Deviation (1e-4) 25.0 34.2 23.6 41.2 22.2 20.8 19.5 19.1 16.0 14.6 13.3 14.0 35.8 48.1 44.0 62.9 63.6 61.2 34.0 44.0 45.1
Intensity Change (Log2) – – – -0.19 -0.27 -0.62 -0.95 -0.95 -1.18 -1.26 -1.4 -1.46 0.23 0.27 0.2 0.56 0.58 0.45 0.03 0.09 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.9 2.6 2.8 2.4 2.0 3.4 0 0 0 0 0 0
Cytoplasm -2.7 -1.2 -2.2 -4.7 -6.1 -6.5 -5.7 -4.9 -8.0 0 -1.3 -1.3 -1.6 -1.8 -1.9
Endoplasmic Reticulum 0 0 0 4.2 5.4 5.2 4.9 3.7 5.9 0 0 0 4.0 4.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 189.2413 219.7294 210.8792 204.3331 175.8084 208.8594 170.3385 232.3721 205.4332 194.4644 192.2987 197.1501 168.7711 209.0961 190.0786 173.5589 197.354 191.5922
Actin 0.0041 0.0001 0.0001 0 0.0097 0.0001 0.0012 0.0001 0.005 0.0021 0.0002 0.0001 0.0013 0.0001 0.0054 0.0065 0 0.0001
Bud 0.0006 0 0.0001 0 0.0029 0.0001 0.003 0.0001 0.0004 0.0005 0.0002 0 0.0009 0.0001 0.0002 0.0002 0 0
Bud Neck 0.0003 0.0002 0.0002 0.0003 0.0008 0.0004 0.0002 0.0001 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0004
Bud Periphery 0.0004 0 0.0001 0 0.0016 0 0.0013 0 0.0002 0.0006 0.0001 0 0.0002 0 0.0002 0.0002 0 0
Bud Site 0.0005 0.0001 0.0001 0 0.001 0 0.0004 0 0.0002 0.0002 0.0001 0 0.0003 0 0.0009 0.0003 0 0
Cell Periphery 0.0011 0.0006 0.0004 0 0.0027 0.0004 0.0009 0.0001 0.0007 0.0007 0.0006 0.0001 0.0005 0 0.0004 0.0012 0 0
Cytoplasm 0.9502 0.9953 0.9744 0.9952 0.8902 0.9728 0.9014 0.9911 0.9463 0.8866 0.9108 0.9945 0.9505 0.9981 0.9359 0.911 0.9951 0.9966
Cytoplasmic Foci 0.0026 0.0001 0.0056 0.0001 0.0027 0.0062 0.0096 0.0019 0.0079 0.0175 0.0188 0.0002 0.0051 0.0001 0.0058 0.0035 0.0001 0.0005
Eisosomes 0.0001 0 0.0001 0 0.0007 0 0.0004 0 0.0003 0.0001 0.0003 0 0.0002 0 0.0002 0.0004 0 0
Endoplasmic Reticulum 0.0011 0.0002 0.0002 0.0001 0.0008 0.0005 0.0014 0.0001 0.0011 0.0007 0.0003 0.0001 0.0002 0.0001 0.0005 0.0009 0 0.0001
Endosome 0.0017 0 0.0001 0 0.0036 0.0007 0.0036 0 0.0035 0.0029 0.0002 0 0.0007 0 0.0043 0.0023 0 0.0001
Golgi 0.0007 0 0 0 0.0024 0.0001 0.0003 0 0.002 0.0005 0.0001 0 0.0005 0 0.0016 0.0016 0 0
Lipid Particles 0.0021 0 0.001 0 0.0037 0.0016 0.0019 0.0001 0.002 0.0026 0.0041 0 0.002 0 0.0037 0.0148 0 0
Mitochondria 0.0048 0 0.0019 0 0.009 0.0001 0.0044 0.0005 0.0032 0.018 0.0079 0 0.0032 0 0.0083 0.0197 0 0
None 0.0028 0.0002 0.0032 0.0001 0.0045 0.0017 0.0073 0.0021 0.011 0.0065 0.0242 0.0001 0.0041 0.0001 0.0033 0.0076 0.0001 0.0001
Nuclear Periphery 0.003 0.0008 0.0011 0.0007 0.0049 0.0021 0.0269 0.0003 0.0025 0.0166 0.0039 0.0009 0.002 0.0002 0.0022 0.0029 0.0008 0.0004
Nucleolus 0.0073 0.0002 0.004 0 0.02 0.0006 0.0022 0.0011 0.0036 0.007 0.0113 0 0.0102 0 0.0145 0.0056 0 0
Nucleus 0.0091 0.0014 0.003 0.0031 0.0209 0.0046 0.0238 0.0012 0.0036 0.0127 0.0054 0.0032 0.0109 0.0009 0.0051 0.0063 0.0036 0.0012
Peroxisomes 0.0006 0 0.0001 0 0.0012 0.0001 0.0004 0 0.0006 0.0002 0.0006 0 0.002 0 0.0015 0.0038 0 0
Punctate Nuclear 0.001 0 0.0005 0 0.0009 0.0043 0.0012 0.0002 0.0004 0.0006 0.0007 0 0.0006 0 0.0015 0.0006 0 0
Vacuole 0.0041 0.0006 0.0013 0.0002 0.0096 0.0031 0.0056 0.0009 0.0031 0.01 0.0044 0.0004 0.0026 0.0001 0.0026 0.0052 0.0001 0.0002
Vacuole Periphery 0.0017 0 0.0024 0 0.0063 0.0004 0.0025 0.0001 0.0022 0.0133 0.0054 0.0001 0.0019 0 0.0016 0.0054 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 561.7856 373.4322 444.6386 366.6731 334.3075 400.2306 207.6673 209.4366 308.2484 282.1824
Translational Efficiency 1.2499 1.2187 1.0781 1.5295 1.3461 1.3741 1.3167 1.3197 1.2099 1.2921

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
966 315 1940 844 1495 612 195 403 2461 927 2135 1247

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8285.25 10617.43 7162.94 6636.77 8353.48 9875.39 7758.30 7565.05 8326.70 10127.54 7217.32 6936.77
Standard Deviation 1852.30 2652.82 1577.61 1964.17 2044.51 2129.64 1706.88 1884.19 1971.58 2347.15 1599.08 1986.70
Intensity Change Log 2 — 0.357817 -0.209993 -0.320064 — 0.241460 -0.106637 -0.143028 — 0.300573 -0.157178 -0.228469

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000104 0.000137 0.000053 0.001239 0.000052 0.000111 0.000210 0.000133 0.000072 0.000120 0.000067 0.000882
Bud Neck 0.000515 0.001351 0.000848 0.001570 0.000304 0.001431 0.010652 0.001463 0.000387 0.001404 0.001744 0.001536
Bud Site 0.002175 0.002777 0.001113 0.004889 0.001057 0.001424 0.002643 0.005632 0.001496 0.001884 0.001253 0.005129
Cell Periphery 0.009231 0.012079 0.001396 0.003895 0.005204 0.004989 0.007394 0.006624 0.006785 0.007398 0.001944 0.004777
Cytoplasm 0.864268* 0.792009* 0.858275* 0.811299* 0.874592* 0.773270* 0.571026* 0.814859* 0.870540* 0.779638* 0.832039* 0.812450*
Cytoplasmic Foci 0.007543 0.016219 0.006177 0.012024 0.004859 0.013714 0.033377 0.015128 0.005913 0.014565 0.008661 0.013027
Eisosomes 0.000002 0.000004 0.000001 0.000003 0.000001 0.000003 0.000008 0.000003 0.000001 0.000003 0.000002 0.000003
Endoplasmic Reticulum 0.000071 0.000035 0.000024 0.000040 0.000057 0.000038 0.000065 0.000010 0.000063 0.000037 0.000027 0.000031
Endosome 0.000118 0.000103 0.000085 0.000127 0.000070 0.000135 0.000641 0.000067 0.000089 0.000124 0.000136 0.000108
Golgi 0.008372 0.004321 0.001697 0.000904 0.006185 0.003583 0.005294 0.000658 0.007043 0.003834 0.002026 0.000825
Lipid Particles 0.005491 0.009313 0.001377 0.004207 0.002133 0.005425 0.013475 0.006782 0.003451 0.006746 0.002482 0.005039
Mitochondria 0.000204 0.000276 0.000135 0.000141 0.000228 0.000271 0.000188 0.000047 0.000218 0.000273 0.000140 0.000110
Mitotic Spindle 0.000500 0.000822 0.000656 0.008843 0.000276 0.000721 0.001819 0.000637 0.000364 0.000755 0.000762 0.006191
None 0.007274 0.009116 0.004796 0.004610 0.007804 0.009286 0.002556 0.004648 0.007596 0.009228 0.004592 0.004623
Nuclear Periphery 0.000365 0.000604 0.000293 0.000243 0.000251 0.000464 0.002413 0.000234 0.000296 0.000511 0.000486 0.000240
Nuclear Periphery Foci 0.004193 0.003302 0.001432 0.002179 0.002184 0.003008 0.010261 0.002724 0.002973 0.003108 0.002239 0.002355
Nucleolus 0.000153 0.000114 0.000076 0.000126 0.000066 0.000185 0.000236 0.000071 0.000100 0.000161 0.000090 0.000109
Nucleus 0.057880 0.114633 0.102939 0.104437 0.071695 0.155212* 0.178884* 0.094248 0.066272 0.141423* 0.109875 0.101144
Peroxisomes 0.000008 0.000015 0.000008 0.000027 0.000006 0.000009 0.000021 0.000029 0.000007 0.000011 0.000009 0.000028
Vacuole 0.026709 0.030247 0.017635 0.038611 0.014546 0.025239 0.156178 0.045770 0.019320 0.026941 0.030289 0.040924
Vacuole Periphery 0.004822 0.002526 0.000983 0.000584 0.008430 0.001480 0.002658 0.000233 0.007014 0.001836 0.001136 0.000471

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.27 1.19 -1.18 -1.11 -1.23 -3.50 -8.50 -7.75 -2.48 3.96 -3.14 -1.78 -1.30 -1.17 -1.25
Bud Neck -4.00 -6.39 -8.16 -2.77 -4.31 -5.87 -10.54 -9.50 -2.35 8.82 -7.00 -11.58 -11.89 -3.14 0.46
Bud Site -0.72 1.02 -3.86 -4.18 -7.14 -1.38 -5.34 -5.17 -4.76 -1.13 -1.20 -0.57 -7.23 -7.18 -8.03
Cell Periphery -1.20 8.50 6.20 4.25 -4.17 0.12 -1.52 0.68 0.58 2.02 -0.65 8.81 5.03 4.29 -4.31
Cytoplasm 6.92 5.64 12.41 1.90 9.06 13.55 23.07 13.15 1.83 -12.52 14.89 13.94 18.73 2.10 6.87
Cytoplasmic Foci -5.01 -1.37 -6.10 -1.62 -5.79 -7.12 -10.67 -6.55 -4.38 1.17 -8.56 -8.87 -9.20 -4.56 -6.71
Eisosomes -1.90 2.02 -2.83 0.45 -3.98 -3.50 -9.83 -6.42 -1.23 6.77 -3.64 -5.18 -5.62 -0.49 -3.17
Endoplasmic Reticulum 2.41 3.85 1.68 -0.66 -1.58 1.30 -1.48 3.93 4.30 7.42 2.44 3.53 2.58 0.47 -0.22
Endosome 0.02 -0.34 -2.83 -2.16 -2.70 -3.10 -5.75 -2.90 0.12 4.81 -2.53 -5.07 -4.44 -1.51 -0.63
Golgi 4.46 8.12 9.37 5.34 6.49 3.85 2.28 9.41 7.30 5.95 5.91 10.08 12.99 8.64 9.40
Lipid Particles -1.52 4.89 -2.48 0.26 -6.22 -3.31 -7.19 -5.75 -2.99 0.63 -3.18 0.75 -6.63 -1.88 -7.16
Mitochondria -0.65 0.39 0.50 1.38 0.45 -0.43 0.10 3.69 3.58 6.17 -0.76 1.00 2.01 2.85 2.83
Mitotic Spindle -1.06 -2.07 -4.83 -4.60 -4.69 -2.25 -2.12 -2.88 -2.55 -1.91 -2.18 -3.32 -5.35 -5.11 -5.08
None -0.44 3.59 3.66 2.21 0.89 -0.40 7.01 4.25 5.12 -4.12 -0.63 5.41 5.32 5.01 0.75
Nuclear Periphery -1.76 -1.93 -1.71 1.31 0.19 -5.52 -6.85 -4.46 2.19 6.30 -3.94 -7.40 -4.56 1.99 4.01
Nuclear Periphery Foci 0.72 4.79 -0.15 -0.76 -3.75 -2.26 -3.69 -4.24 -2.33 2.01 -0.93 1.29 -3.06 -2.01 -3.83
Nucleolus 0.20 0.77 -0.23 -0.81 -4.67 -2.21 -9.57 -3.11 1.54 8.13 -1.49 -0.48 -1.52 0.62 -2.82
Nucleus -7.64 -14.59 -11.07 1.33 1.48 -13.36 -11.13 -5.41 7.30 7.70 -15.42 -17.24 -11.77 6.70 4.10
Peroxisomes -1.70 -0.36 -4.98 -3.08 -5.05 -1.15 -7.16 -3.19 -2.95 -1.04 -1.90 -2.44 -5.57 -4.73 -4.96
Vacuole -1.76 -0.10 -7.62 -4.62 -8.30 -5.54 -13.15 -11.47 -7.06 7.71 -5.32 -8.78 -14.25 -7.93 -6.54
Vacuole Periphery 2.54 5.07 5.81 2.89 4.08 5.76 4.86 6.91 5.74 4.01 6.39 7.44 8.44 5.06 6.42
Standard name
Human Ortholog
Description S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Sam1

Sam1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available