Standard name | |
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Human Ortholog | |
Description | S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 9 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 3 | 3 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 6 | 1 | 1 | 5 | 24 | 47 | 28 | 36 | 23 | 44 | 3 | 8 | 5 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 273 | 140 | 230 | 242 | 345 | 379 | 556 | 540 | 287 | 452 | 338 | 342 | 446 | 526 | 631 | 254 | 275 | 187 | 237 | 136 | 207 | 267 | 493 | 597 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 1 | 6 | 44 | 74 | 38 | 51 | 22 | 62 | 0 | 0 | 1 | 17 | 19 | 11 | 0 | 0 | 1 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 8 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 11 | 0 | 4 | 3 | 0 | 5 | 1 | 0 | 1 | 0 | 1 | 7 | 3 |
Unique Cell Count | 275 | 141 | 230 | 250 | 347 | 387 | 611 | 630 | 343 | 518 | 375 | 446 | 446 | 530 | 636 | 257 | 279 | 190 | 248 | 141 | 216 | 275 | 528 | 626 |
Labelled Cell Count | 275 | 145 | 236 | 252 | 347 | 393 | 630 | 667 | 361 | 546 | 396 | 475 | 449 | 538 | 645 | 275 | 303 | 201 | 248 | 141 | 216 | 275 | 528 | 626 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 133.9 | 178.6 | 146.7 | 128.3 | 121.6 | 95.4 | 76.1 | 76.1 | 64.7 | 61.4 | 55.6 | 53.2 | 172.2 | 177.2 | 168.0 | 216.4 | 218.6 | 200.4 | 149.7 | 156.6 | 175.6 |
Std Deviation (1e-4) | 25.0 | 34.2 | 23.6 | 41.2 | 22.2 | 20.8 | 19.5 | 19.1 | 16.0 | 14.6 | 13.3 | 14.0 | 35.8 | 48.1 | 44.0 | 62.9 | 63.6 | 61.2 | 34.0 | 44.0 | 45.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.27 | -0.62 | -0.95 | -0.95 | -1.18 | -1.26 | -1.4 | -1.46 | 0.23 | 0.27 | 0.2 | 0.56 | 0.58 | 0.45 | 0.03 | 0.09 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.9 | 2.6 | 2.8 | 2.4 | 2.0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | -1.2 | -2.2 | -4.7 | -6.1 | -6.5 | -5.7 | -4.9 | -8.0 | 0 | -1.3 | -1.3 | -1.6 | -1.8 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 4.2 | 5.4 | 5.2 | 4.9 | 3.7 | 5.9 | 0 | 0 | 0 | 4.0 | 4.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 189.2413 | 219.7294 | 210.8792 | 204.3331 | 175.8084 | 208.8594 | 170.3385 | 232.3721 | 205.4332 | 194.4644 | 192.2987 | 197.1501 | 168.7711 | 209.0961 | 190.0786 | 173.5589 | 197.354 | 191.5922 |
Actin | 0.0041 | 0.0001 | 0.0001 | 0 | 0.0097 | 0.0001 | 0.0012 | 0.0001 | 0.005 | 0.0021 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0054 | 0.0065 | 0 | 0.0001 |
Bud | 0.0006 | 0 | 0.0001 | 0 | 0.0029 | 0.0001 | 0.003 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0016 | 0 | 0.0013 | 0 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0 | 0 |
Bud Site | 0.0005 | 0.0001 | 0.0001 | 0 | 0.001 | 0 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0009 | 0.0003 | 0 | 0 |
Cell Periphery | 0.0011 | 0.0006 | 0.0004 | 0 | 0.0027 | 0.0004 | 0.0009 | 0.0001 | 0.0007 | 0.0007 | 0.0006 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0012 | 0 | 0 |
Cytoplasm | 0.9502 | 0.9953 | 0.9744 | 0.9952 | 0.8902 | 0.9728 | 0.9014 | 0.9911 | 0.9463 | 0.8866 | 0.9108 | 0.9945 | 0.9505 | 0.9981 | 0.9359 | 0.911 | 0.9951 | 0.9966 |
Cytoplasmic Foci | 0.0026 | 0.0001 | 0.0056 | 0.0001 | 0.0027 | 0.0062 | 0.0096 | 0.0019 | 0.0079 | 0.0175 | 0.0188 | 0.0002 | 0.0051 | 0.0001 | 0.0058 | 0.0035 | 0.0001 | 0.0005 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0005 | 0.0014 | 0.0001 | 0.0011 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0009 | 0 | 0.0001 |
Endosome | 0.0017 | 0 | 0.0001 | 0 | 0.0036 | 0.0007 | 0.0036 | 0 | 0.0035 | 0.0029 | 0.0002 | 0 | 0.0007 | 0 | 0.0043 | 0.0023 | 0 | 0.0001 |
Golgi | 0.0007 | 0 | 0 | 0 | 0.0024 | 0.0001 | 0.0003 | 0 | 0.002 | 0.0005 | 0.0001 | 0 | 0.0005 | 0 | 0.0016 | 0.0016 | 0 | 0 |
Lipid Particles | 0.0021 | 0 | 0.001 | 0 | 0.0037 | 0.0016 | 0.0019 | 0.0001 | 0.002 | 0.0026 | 0.0041 | 0 | 0.002 | 0 | 0.0037 | 0.0148 | 0 | 0 |
Mitochondria | 0.0048 | 0 | 0.0019 | 0 | 0.009 | 0.0001 | 0.0044 | 0.0005 | 0.0032 | 0.018 | 0.0079 | 0 | 0.0032 | 0 | 0.0083 | 0.0197 | 0 | 0 |
None | 0.0028 | 0.0002 | 0.0032 | 0.0001 | 0.0045 | 0.0017 | 0.0073 | 0.0021 | 0.011 | 0.0065 | 0.0242 | 0.0001 | 0.0041 | 0.0001 | 0.0033 | 0.0076 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.003 | 0.0008 | 0.0011 | 0.0007 | 0.0049 | 0.0021 | 0.0269 | 0.0003 | 0.0025 | 0.0166 | 0.0039 | 0.0009 | 0.002 | 0.0002 | 0.0022 | 0.0029 | 0.0008 | 0.0004 |
Nucleolus | 0.0073 | 0.0002 | 0.004 | 0 | 0.02 | 0.0006 | 0.0022 | 0.0011 | 0.0036 | 0.007 | 0.0113 | 0 | 0.0102 | 0 | 0.0145 | 0.0056 | 0 | 0 |
Nucleus | 0.0091 | 0.0014 | 0.003 | 0.0031 | 0.0209 | 0.0046 | 0.0238 | 0.0012 | 0.0036 | 0.0127 | 0.0054 | 0.0032 | 0.0109 | 0.0009 | 0.0051 | 0.0063 | 0.0036 | 0.0012 |
Peroxisomes | 0.0006 | 0 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0002 | 0.0006 | 0 | 0.002 | 0 | 0.0015 | 0.0038 | 0 | 0 |
Punctate Nuclear | 0.001 | 0 | 0.0005 | 0 | 0.0009 | 0.0043 | 0.0012 | 0.0002 | 0.0004 | 0.0006 | 0.0007 | 0 | 0.0006 | 0 | 0.0015 | 0.0006 | 0 | 0 |
Vacuole | 0.0041 | 0.0006 | 0.0013 | 0.0002 | 0.0096 | 0.0031 | 0.0056 | 0.0009 | 0.0031 | 0.01 | 0.0044 | 0.0004 | 0.0026 | 0.0001 | 0.0026 | 0.0052 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0017 | 0 | 0.0024 | 0 | 0.0063 | 0.0004 | 0.0025 | 0.0001 | 0.0022 | 0.0133 | 0.0054 | 0.0001 | 0.0019 | 0 | 0.0016 | 0.0054 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 561.7856 | 373.4322 | 444.6386 | 366.6731 | 334.3075 | 400.2306 | 207.6673 | 209.4366 | 308.2484 | 282.1824 |
Translational Efficiency | 1.2499 | 1.2187 | 1.0781 | 1.5295 | 1.3461 | 1.3741 | 1.3167 | 1.3197 | 1.2099 | 1.2921 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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