Standard name | |
---|---|
Human Ortholog | |
Description | Structural constituent of the cell wall; attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress; null mutant is sensitive to the antifungal agent polyhexamethylene biguanide, resistant to zymolyase treatment and has increased chitin deposition |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | 7 | 15 | 13 | 38 | 40 | 25 | 28 | 17 | 14 | 18 | 1 | 0 | 1 | 1 | 0 | 0 | 3 | 10 | 7 | 4 | 11 | 10 |
Bud | 1 | 2 | 0 | 7 | 7 | 10 | 12 | 10 | 14 | 15 | 9 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 11 | 1 | 4 | 12 | 13 |
Bud Neck | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 7 | 6 | 1 | 2 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 11 | 16 | 14 | 14 | 50 | 48 | 23 | 26 | 17 | 19 | 25 | 7 | 8 | 7 | 0 | 3 | 2 | 0 | 5 | 4 | 3 | 3 | 3 |
Cytoplasm | 48 | 267 | 188 | 174 | 133 | 173 | 236 | 308 | 239 | 250 | 289 | 351 | 159 | 139 | 221 | 105 | 96 | 73 | 26 | 140 | 56 | 48 | 26 | 18 |
Endoplasmic Reticulum | 37 | 106 | 89 | 62 | 111 | 181 | 191 | 154 | 68 | 42 | 29 | 29 | 75 | 21 | 18 | 10 | 7 | 11 | 75 | 208 | 215 | 89 | 136 | 138 |
Endosome | 0 | 1 | 2 | 3 | 2 | 7 | 1 | 2 | 2 | 3 | 1 | 1 | 0 | 1 | 1 | 10 | 1 | 2 | 0 | 8 | 4 | 3 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 17 | 16 | 0 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 2 | 9 | 0 | 2 | 8 | 10 | 6 | 4 | 29 | 14 | 15 | 23 | 1 | 0 | 0 | 3 | 1 | 2 | 2 | 24 | 17 | 17 | 9 | 3 |
Nucleus | 0 | 1 | 0 | 0 | 5 | 2 | 4 | 17 | 24 | 19 | 17 | 12 | 1 | 0 | 1 | 28 | 11 | 24 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 4 | 26 | 5 | 9 | 28 | 64 | 62 | 116 | 83 | 83 | 70 | 120 | 3 | 3 | 4 | 233 | 150 | 156 | 7 | 37 | 14 | 12 | 8 | 10 |
Unique Cell Count | 83 | 399 | 283 | 262 | 280 | 439 | 511 | 570 | 422 | 388 | 400 | 513 | 226 | 161 | 244 | 351 | 250 | 251 | 121 | 464 | 338 | 189 | 219 | 217 |
Labelled Cell Count | 93 | 429 | 310 | 288 | 325 | 537 | 601 | 661 | 514 | 463 | 467 | 606 | 248 | 172 | 254 | 399 | 288 | 289 | 121 | 464 | 338 | 189 | 219 | 217 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 6.0 | 7.5 | 7.5 | 7.2 | 9.6 | 9.3 | 9.3 | 8.0 | 8.3 | 8.3 | 7.7 | 7.3 | 7.0 | 6.5 | 21.3 | 25.4 | 24.3 | 8.0 | 11.0 | 12.6 |
Std Deviation (1e-4) | 1.4 | 1.5 | 1.4 | 2.0 | 1.9 | 3.4 | 2.2 | 2.3 | 2.1 | 2.4 | 3.0 | 2.4 | 1.6 | 1.3 | 2.0 | 16.6 | 17.8 | 16.4 | 2.4 | 4.2 | 6.1 |
Intensity Change (Log2) | – | – | – | -0.0 | -0.06 | 0.36 | 0.32 | 0.31 | 0.09 | 0.15 | 0.14 | 0.04 | -0.04 | -0.1 | -0.2 | 1.51 | 1.76 | 1.7 | 0.1 | 0.56 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.9 | 1.4 | 3.4 | 3.1 | 1.4 | 2.5 | 1.3 | 0.8 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.3 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.2 | -0.3 | 2.6 | 1.9 | -1.1 | 0.3 | -0.8 | -0.5 | -0.5 | -1.4 | -0.3 | -1.6 | -4.5 | -2.8 | -3.1 |
Cytoplasm | 0 | -4.5 | -7.1 | -5.5 | -3.5 | -2.6 | -0.5 | 1.6 | 0.6 | 0.9 | 4.6 | 6.6 | -9.2 | -6.5 | -8.6 |
Endoplasmic Reticulum | -2.0 | 2.0 | 2.7 | 1.7 | -1.4 | -4.8 | -6.7 | -8.2 | -9.8 | 0.4 | -4.3 | -6.8 | -9.9 | -8.6 | -8.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.3 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.5 | 3.2 | 3.3 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.9 | 4.1 | 3.8 | 3.5 | 0 | 0 | 0 | 0 | 4.9 | 3.6 | 5.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.2 | 4.2 | 5.7 | 5.0 | 7.3 | 7.0 | 7.4 | 6.5 | 8.0 | 0 | 0 | 0 | 16.7 | 14.8 | 15.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7201 | 3.9267 | 3.1483 | 1.6231 | 1.7572 | 4.048 | 9.2965 | 9.2868 | 8.1285 | 8.2342 | 7.786 | 9.8401 | 6.9415 | 7.0856 | 5.7051 | 5.3306 | 5.2142 | 6.9284 |
Actin | 0.003 | 0.0024 | 0.0068 | 0.005 | 0.0123 | 0.0047 | 0.0122 | 0.0071 | 0.0042 | 0.0213 | 0.0013 | 0.0059 | 0.0068 | 0.0017 | 0.0082 | 0.0015 | 0.0079 | 0.0079 |
Bud | 0.0003 | 0.0111 | 0.0031 | 0.0029 | 0.0321 | 0.0018 | 0.0004 | 0.0011 | 0.0008 | 0.0015 | 0.0009 | 0.0006 | 0.0001 | 0.0007 | 0.0008 | 0.0008 | 0.0038 | 0.0007 |
Bud Neck | 0.0014 | 0.0009 | 0.0023 | 0.0017 | 0.0018 | 0.0027 | 0.0011 | 0.0006 | 0.0028 | 0.0017 | 0.0011 | 0.0047 | 0.0003 | 0.0006 | 0.0008 | 0.0008 | 0.0012 | 0.0038 |
Bud Periphery | 0.0003 | 0.0035 | 0.0017 | 0.0172 | 0.0135 | 0.002 | 0.0009 | 0.0027 | 0.0027 | 0.0025 | 0.0019 | 0.0017 | 0.0003 | 0.0015 | 0.0022 | 0.0019 | 0.0189 | 0.0011 |
Bud Site | 0.002 | 0.0312 | 0.0058 | 0.0031 | 0.0017 | 0.0006 | 0.0023 | 0.0091 | 0.0062 | 0.0446 | 0.0009 | 0.0022 | 0.0003 | 0.0093 | 0.0055 | 0.0009 | 0.0006 | 0.0007 |
Cell Periphery | 0.0011 | 0.0053 | 0.0028 | 0.0013 | 0.0015 | 0.001 | 0.0019 | 0.0022 | 0.0028 | 0.0027 | 0.0021 | 0.0009 | 0.0013 | 0.0038 | 0.0039 | 0.0013 | 0.0038 | 0.0009 |
Cytoplasm | 0.1967 | 0.2869 | 0.2728 | 0.3176 | 0.4628 | 0.3249 | 0.1346 | 0.2398 | 0.269 | 0.2327 | 0.3616 | 0.2216 | 0.2557 | 0.3277 | 0.3021 | 0.3275 | 0.3732 | 0.3434 |
Cytoplasmic Foci | 0.0101 | 0.0096 | 0.0106 | 0.0316 | 0.0142 | 0.015 | 0.0136 | 0.0083 | 0.0134 | 0.0273 | 0.0134 | 0.0098 | 0.0171 | 0.0067 | 0.0099 | 0.0163 | 0.0092 | 0.0072 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.6714 | 0.5097 | 0.5626 | 0.4115 | 0.3347 | 0.5245 | 0.6697 | 0.5774 | 0.5352 | 0.5357 | 0.4586 | 0.6327 | 0.5497 | 0.5087 | 0.5206 | 0.4553 | 0.3366 | 0.5417 |
Endosome | 0.0186 | 0.0307 | 0.0273 | 0.0415 | 0.021 | 0.0293 | 0.0418 | 0.0315 | 0.0435 | 0.0389 | 0.0344 | 0.018 | 0.0291 | 0.0175 | 0.0206 | 0.0264 | 0.0156 | 0.0151 |
Golgi | 0.0014 | 0.0021 | 0.0022 | 0.0081 | 0.0016 | 0.0013 | 0.0065 | 0.0067 | 0.0071 | 0.0114 | 0.0057 | 0.0021 | 0.0042 | 0.0015 | 0.0037 | 0.0018 | 0.007 | 0.0014 |
Lipid Particles | 0.0066 | 0.0063 | 0.0073 | 0.0065 | 0.0039 | 0.0038 | 0.0143 | 0.0057 | 0.0121 | 0.012 | 0.0121 | 0.0051 | 0.0073 | 0.0073 | 0.0077 | 0.0071 | 0.0039 | 0.0026 |
Mitochondria | 0.0027 | 0.0012 | 0.0008 | 0.0065 | 0.0016 | 0.001 | 0.0085 | 0.0037 | 0.0031 | 0.0029 | 0.0131 | 0.001 | 0.0016 | 0.0012 | 0.0023 | 0.0019 | 0.0332 | 0.0017 |
None | 0.0314 | 0.0393 | 0.0392 | 0.093 | 0.0523 | 0.0398 | 0.0352 | 0.0681 | 0.061 | 0.0261 | 0.0651 | 0.0678 | 0.0769 | 0.0693 | 0.0719 | 0.1005 | 0.1469 | 0.0475 |
Nuclear Periphery | 0.0261 | 0.0296 | 0.0246 | 0.0246 | 0.0187 | 0.0227 | 0.0209 | 0.0179 | 0.0122 | 0.0089 | 0.0068 | 0.01 | 0.0226 | 0.0151 | 0.0204 | 0.028 | 0.014 | 0.0105 |
Nucleolus | 0.0031 | 0.0006 | 0.0008 | 0.0006 | 0.0005 | 0.0005 | 0.0016 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0002 |
Nucleus | 0.0081 | 0.0093 | 0.0091 | 0.0065 | 0.0113 | 0.0077 | 0.0125 | 0.0048 | 0.0052 | 0.002 | 0.0037 | 0.0042 | 0.0074 | 0.0083 | 0.0052 | 0.0119 | 0.0051 | 0.0052 |
Peroxisomes | 0.0003 | 0.0001 | 0.0003 | 0.0016 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0011 | 0.0179 | 0.003 | 0.0022 | 0.0064 | 0.0001 | 0.0015 | 0.0025 | 0.0005 | 0.0004 |
Punctate Nuclear | 0.0071 | 0.0034 | 0.006 | 0.0081 | 0.0048 | 0.0075 | 0.0058 | 0.0008 | 0.0021 | 0.0015 | 0.0051 | 0.0025 | 0.0034 | 0.0032 | 0.0027 | 0.0023 | 0.0066 | 0.0015 |
Vacuole | 0.0073 | 0.0148 | 0.0123 | 0.0092 | 0.0082 | 0.0075 | 0.013 | 0.0105 | 0.0131 | 0.0071 | 0.0072 | 0.0059 | 0.0077 | 0.0141 | 0.0075 | 0.0094 | 0.0093 | 0.0059 |
Vacuole Periphery | 0.0012 | 0.0018 | 0.0014 | 0.0019 | 0.001 | 0.0011 | 0.0023 | 0.0012 | 0.0014 | 0.0008 | 0.0013 | 0.0006 | 0.0014 | 0.0009 | 0.002 | 0.0014 | 0.002 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.26 | 16.7619 | 29.6601 | 114.7459 | 29.9213 | 10.0909 | 17.5998 | 13.6385 | 74.6367 | 62.9567 |
Translational Efficiency | 1.895 | 1.1047 | 2.1935 | 1.3741 | 1.9017 | 3.7536 | 1.4405 | 2.1613 | 2.0908 | 1.7604 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
222 | 1055 | 2256 | 1274 | 2444 | 1921 | 240 | 905 | 2666 | 2976 | 2496 | 2179 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 754.08 | 712.36 | 1015.07 | 1275.69 | 780.17 | 916.87 | 1079.59 | 1268.65 | 778.00 | 844.37 | 1021.27 | 1272.77 |
Standard Deviation | 134.75 | 422.40 | 140.25 | 497.56 | 137.91 | 142.39 | 382.19 | 431.88 | 137.84 | 293.10 | 179.41 | 471.41 |
Intensity Change Log 2 | — | -0.082111 | 0.428790 | 0.758488 | — | 0.232929 | 0.468623 | 0.701434 | — | 0.086657 | 0.449183 | 0.729758 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000396 | 0.001168 | 0.000934 | 0.002812 | 0.000274 | 0.000622 | 0.000890 | 0.003009 | 0.000284 | 0.000815 | 0.000930 | 0.002894 |
Bud Neck | 0.017089 | 0.006118 | 0.003894 | 0.012992 | 0.005664 | 0.005818 | 0.004230 | 0.008798 | 0.006616 | 0.005924 | 0.003927 | 0.011250 |
Bud Site | 0.016907 | 0.019479 | 0.007226 | 0.047075 | 0.007703 | 0.006387 | 0.007044 | 0.050547 | 0.008470 | 0.011028 | 0.007208 | 0.048517 |
Cell Periphery | 0.003345 | 0.001024 | 0.000836 | 0.004209 | 0.001590 | 0.001742 | 0.006244 | 0.011150 | 0.001736 | 0.001488 | 0.001356 | 0.007092 |
Cytoplasm | 0.321627* | 0.325615* | 0.308144* | 0.222318* | 0.447013* | 0.354695* | 0.148949* | 0.182410* | 0.436572* | 0.344386* | 0.292837* | 0.205743* |
Cytoplasmic Foci | 0.127625 | 0.159059 | 0.017755 | 0.037234 | 0.069606 | 0.085081 | 0.022329 | 0.023155 | 0.074437 | 0.111306 | 0.018195 | 0.031387 |
Eisosomes | 0.001093 | 0.000255 | 0.000161 | 0.000154 | 0.000241 | 0.000848 | 0.000439 | 0.000311 | 0.000312 | 0.000638 | 0.000188 | 0.000219 |
Endoplasmic Reticulum | 0.197663* | 0.203841* | 0.375853* | 0.341408* | 0.212695* | 0.345404* | 0.536537* | 0.514594* | 0.211444* | 0.295219* | 0.391303* | 0.413337* |
Endosome | 0.035952 | 0.032028 | 0.005999 | 0.027396 | 0.007080 | 0.009744 | 0.010416 | 0.015590 | 0.009484 | 0.017643 | 0.006424 | 0.022493 |
Golgi | 0.008349 | 0.011413 | 0.000791 | 0.009212 | 0.003070 | 0.009582 | 0.000809 | 0.004956 | 0.003510 | 0.010231 | 0.000793 | 0.007445 |
Lipid Particles | 0.028579 | 0.048567 | 0.011631 | 0.034280 | 0.028070 | 0.047951 | 0.021067 | 0.039695 | 0.028112 | 0.048169 | 0.012538 | 0.036529 |
Mitochondria | 0.001193 | 0.006866 | 0.000846 | 0.004836 | 0.002958 | 0.004815 | 0.000251 | 0.001197 | 0.002811 | 0.005542 | 0.000788 | 0.003324 |
Mitotic Spindle | 0.000050 | 0.001070 | 0.001051 | 0.019367 | 0.000251 | 0.000642 | 0.000186 | 0.006132 | 0.000234 | 0.000794 | 0.000968 | 0.013870 |
None | 0.000212 | 0.019610 | 0.001824 | 0.003588 | 0.010904 | 0.004768 | 0.000420 | 0.003124 | 0.010014 | 0.010029 | 0.001689 | 0.003395 |
Nuclear Periphery | 0.001598 | 0.007974 | 0.019199 | 0.008880 | 0.008179 | 0.004348 | 0.010621 | 0.001801 | 0.007631 | 0.005633 | 0.018374 | 0.005940 |
Nuclear Periphery Foci | 0.002645 | 0.020008 | 0.036493 | 0.048016 | 0.007847 | 0.005251 | 0.058543 | 0.030018 | 0.007413 | 0.010482 | 0.038613 | 0.040541 |
Nucleolus | 0.000297 | 0.003068 | 0.000182 | 0.000368 | 0.000847 | 0.000551 | 0.000159 | 0.000257 | 0.000802 | 0.001443 | 0.000180 | 0.000322 |
Nucleus | 0.021574 | 0.047698 | 0.145073* | 0.026987 | 0.151143* | 0.067227 | 0.056972 | 0.018463 | 0.140353* | 0.060304 | 0.136602* | 0.023447 |
Peroxisomes | 0.002849 | 0.003450 | 0.000364 | 0.001028 | 0.001197 | 0.003306 | 0.000446 | 0.000530 | 0.001334 | 0.003357 | 0.000372 | 0.000821 |
Vacuole | 0.205051* | 0.074803 | 0.061504 | 0.140865 | 0.032843 | 0.039978 | 0.113214 | 0.083016 | 0.047183 | 0.052323 | 0.066476 | 0.116838 |
Vacuole Periphery | 0.005908 | 0.006888 | 0.000239 | 0.006977 | 0.000823 | 0.001243 | 0.000234 | 0.001247 | 0.001247 | 0.003244 | 0.000238 | 0.004597 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.19 | -3.06 | -7.15 | -4.13 | -4.98 | -2.52 | -3.40 | -7.84 | -6.54 | -5.07 | -4.28 | -4.25 | -10.72 | -7.88 | -6.81 |
Bud Neck | 3.86 | 4.56 | 1.00 | -6.97 | -9.20 | -0.17 | 0.75 | -4.06 | -3.36 | -3.87 | 0.90 | 3.58 | -6.50 | -7.30 | -10.36 |
Bud Site | -0.61 | 2.42 | -6.52 | -7.33 | -11.74 | 1.00 | -0.41 | -10.07 | -10.30 | -9.13 | -1.91 | 0.52 | -14.94 | -13.76 | -15.07 |
Cell Periphery | 3.34 | 3.72 | -0.76 | -4.95 | -5.46 | -0.32 | -1.73 | -7.31 | -7.04 | -1.36 | 0.69 | 0.96 | -7.59 | -8.02 | -8.07 |
Cytoplasm | -0.18 | 0.74 | 4.26 | 7.09 | 6.94 | 11.39 | 20.75 | 24.40 | 14.69 | -2.82 | 12.94 | 19.67 | 27.41 | 15.63 | 8.93 |
Cytoplasmic Foci | -2.91 | 11.60 | 9.47 | 21.10 | -8.22 | -4.31 | 11.52 | 14.95 | 16.47 | 0.06 | -10.64 | 24.21 | 16.54 | 25.11 | -7.34 |
Eisosomes | 3.01 | 3.38 | 3.43 | 2.43 | 0.91 | -5.86 | -2.49 | -1.07 | 4.77 | 1.52 | -4.47 | 4.35 | 3.00 | 5.94 | -0.81 |
Endoplasmic Reticulum | -0.23 | -7.22 | -3.96 | -6.42 | 5.50 | -12.70 | -12.04 | -17.11 | -7.81 | 1.38 | -9.46 | -17.56 | -14.83 | -6.67 | 0.84 |
Endosome | 1.05 | 9.02 | 2.91 | 3.07 | -13.50 | -4.79 | -2.76 | -8.29 | -5.40 | -3.42 | -10.24 | 5.60 | -11.67 | -3.23 | -15.34 |
Golgi | -1.53 | 4.28 | 0.23 | 2.42 | -6.26 | -9.67 | 9.49 | -1.67 | 4.41 | -4.17 | -11.46 | 9.78 | -3.75 | 4.01 | -7.47 |
Lipid Particles | -4.85 | 4.99 | -1.29 | 4.42 | -9.39 | -8.66 | 2.78 | -3.02 | 2.14 | -4.33 | -10.58 | 12.62 | -3.53 | 4.84 | -11.28 |
Mitochondria | -7.32 | 1.87 | -2.79 | 2.04 | -3.14 | -3.25 | 10.44 | 5.25 | 6.31 | -4.47 | -5.63 | 8.52 | -0.25 | 3.37 | -3.46 |
Mitotic Spindle | -4.29 | -4.21 | -8.50 | -8.07 | -7.87 | -0.85 | -0.22 | -4.21 | -3.83 | -4.20 | -1.74 | -3.46 | -9.36 | -8.90 | -8.54 |
None | -7.02 | -4.01 | -3.74 | 5.64 | -1.70 | 4.88 | 9.36 | 5.86 | 1.70 | -3.55 | -0.00 | 7.82 | 5.82 | 5.62 | -2.39 |
Nuclear Periphery | -4.89 | -12.60 | -4.82 | -0.23 | 6.65 | 5.45 | -1.21 | 9.48 | 5.16 | 5.02 | 2.84 | -9.01 | 2.27 | -0.01 | 10.12 |
Nuclear Periphery Foci | -9.45 | -20.35 | -15.17 | -8.29 | -3.16 | 4.54 | -8.86 | -8.00 | -9.02 | 4.38 | -3.99 | -21.12 | -15.89 | -14.07 | -0.43 |
Nucleolus | -7.02 | 2.47 | -1.42 | 6.85 | -4.61 | 2.21 | 7.31 | 2.74 | 0.94 | -1.23 | -3.80 | 8.22 | 4.37 | 6.34 | -2.68 |
Nucleus | -7.92 | -29.80 | -4.59 | 4.42 | 27.32 | 22.32 | 12.03 | 36.57 | 16.53 | 5.93 | 23.59 | 0.12 | 34.89 | 14.85 | 29.74 |
Peroxisomes | -1.05 | 4.66 | 3.42 | 8.17 | -3.95 | -4.42 | 5.37 | 4.84 | 5.90 | -0.81 | -6.07 | 7.75 | 3.68 | 7.87 | -4.21 |
Vacuole | 7.40 | 7.76 | 2.26 | -13.09 | -14.97 | -3.18 | -8.36 | -11.45 | -9.95 | 1.87 | -1.92 | -8.77 | -19.48 | -18.72 | -12.77 |
Vacuole Periphery | -0.90 | 6.09 | -0.02 | 0.74 | -4.94 | -4.21 | 11.12 | -2.59 | 0.12 | -6.53 | -9.00 | 10.41 | -3.99 | -1.04 | -5.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|