Standard name | |
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Human Ortholog | |
Description | Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 4 | 1 | 4 | 2 | 4 | 3 | 0 | 3 | 2 | 3 | 3 | 12 | 13 | 10 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 19 | 119 | 115 | 77 | 104 | 116 | 138 | 174 | 109 | 92 | 69 | 100 | 73 | 156 | 150 | 16 | 10 | 3 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleus | 3 | 31 | 13 | 7 | 3 | 5 | 2 | 3 | 7 | 4 | 8 | 5 | 9 | 8 | 13 | 21 | 26 | 17 | 1 | 20 | 8 | 0 | 3 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 19 | 160 | 186 | 113 | 194 | 203 | 306 | 402 | 189 | 199 | 166 | 205 | 110 | 235 | 237 | 238 | 122 | 90 | 47 | 137 | 169 | 77 | 144 | 172 |
Peroxisomes | 0 | 1 | 0 | 2 | 3 | 0 | 13 | 6 | 1 | 3 | 3 | 3 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 0 | 0 | 3 | 2 | 23 | 21 | 5 | 9 | 6 | 7 | 0 | 3 | 5 | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 3 | 2 | 0 | 4 | 4 |
Unique Cell Count | 20 | 174 | 195 | 118 | 204 | 211 | 330 | 422 | 201 | 215 | 173 | 224 | 118 | 250 | 262 | 252 | 139 | 101 | 51 | 166 | 196 | 85 | 159 | 194 |
Labelled Cell Count | 41 | 314 | 320 | 200 | 312 | 329 | 490 | 612 | 313 | 314 | 255 | 327 | 197 | 418 | 423 | 289 | 166 | 113 | 51 | 166 | 196 | 85 | 159 | 194 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 45.7 | 55.2 | 42.6 | 42.2 | 35.4 | 31.4 | 25.3 | 26.0 | 24.7 | 22.8 | 22.6 | 21.8 | 52.0 | 51.9 | 46.1 | 45.4 | 59.4 | 63.4 | 41.8 | 52.1 | 54.3 |
Std Deviation (1e-4) | 11.3 | 20.1 | 17.9 | 18.3 | 15.5 | 11.5 | 11.9 | 11.8 | 10.1 | 11.6 | 9.6 | 8.7 | 27.3 | 24.9 | 19.8 | 24.9 | 26.3 | 26.7 | 15.4 | 19.8 | 24.3 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.27 | -0.44 | -0.75 | -0.71 | -0.78 | -0.9 | -0.91 | -0.97 | 0.29 | 0.29 | 0.12 | 0.09 | 0.48 | 0.58 | -0.03 | 0.29 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.6 | 1.2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.1 | -1.6 | -0.8 | -3.8 | -4.1 | -1.0 | -3.3 | -3.7 | -2.9 | 0.5 | 0.7 | -0.4 | -12.1 | -9.6 | -9.3 |
Nucleus | -0.3 | -2.6 | -2.1 | -4.0 | -4.3 | -1.4 | -2.4 | -0.8 | -2.2 | 0.3 | -1.7 | -0.8 | 0.7 | 3.4 | 2.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.2 | -0.1 | 0.4 | -1.2 | -0.1 | -0.6 | -1.2 | 0.3 | -1.6 | -0.8 | -0.6 | -2.0 | -0.4 | -2.6 | -2.0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3.8 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0113 | 0 | 0.0118 | 0 | 0.0004 | 0 | 0.0018 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Neck | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | – | – | – | – | – | – |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Bud Site | 0.0003 | 0 | 0.002 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0.0002 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0009 | 0.0002 | – | – | – | – | – | – |
Cytoplasm | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0436 | 0 | 0.0049 | 0.0004 | 0.0004 | 0.0003 | 0.1256 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0008 | – | – | – | – | – | – |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0006 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Endosome | 0.0066 | 0 | 0.0001 | 0 | 0 | 0 | 0.0043 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Golgi | 0.0083 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0037 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Lipid Particles | 0.1845 | 0 | 0.0008 | 0.0005 | 0.0063 | 0.0013 | 0.0228 | 0.0001 | 0.0003 | 0.0004 | 0.0022 | 0.0009 | – | – | – | – | – | – |
Mitochondria | 0.0069 | 0.0054 | 0.0078 | 0.0028 | 0.3227 | 0.0234 | 0.0133 | 0.0008 | 0.0072 | 0.0072 | 0.2169 | 0.0266 | – | – | – | – | – | – |
None | 0.0038 | 0 | 0 | 0 | 0 | 0 | 0.0054 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Nuclear Periphery | 0.0027 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Nucleolus | 0.6534 | 0.8101 | 0.8994 | 0.9698 | 0.6495 | 0.9414 | 0.755 | 0.8975 | 0.9627 | 0.955 | 0.7268 | 0.9372 | – | – | – | – | – | – |
Nucleus | 0.012 | 0.1823 | 0.0603 | 0.0259 | 0.0114 | 0.0321 | 0.0391 | 0.1002 | 0.028 | 0.0343 | 0.0476 | 0.0323 | – | – | – | – | – | – |
Peroxisomes | 0.052 | 0.0001 | 0.0108 | 0.0001 | 0.0068 | 0.0008 | 0.0224 | 0 | 0.0003 | 0.0003 | 0.0023 | 0.0007 | – | – | – | – | – | – |
Punctate Nuclear | 0.0121 | 0 | 0.0001 | 0 | 0 | 0 | 0.0036 | 0 | 0 | 0.0007 | 0 | 0.0008 | – | – | – | – | – | – |
Vacuole | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.001 | 0.0002 | – | – | – | – | – | – |
Vacuole Periphery | 0.0007 | 0.0007 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0019 | 0.0001 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 447.9167 | 457.7046 | 447.3733 | 334.2323 | 350.3347 | 353.7675 | 305.451 | 272.5829 | 239.3467 | 300.0886 |
Translational Efficiency | 1.2699 | 1.3137 | 1.1413 | 1.4629 | 1.3152 | 1.3377 | 1.3428 | 1.2422 | 1.1869 | 1.2437 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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