Standard name
Human Ortholog
Description Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0.06 0.06 0 0 0.06 0.14 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.92 0.91 0.79 – 0.67 0.57 0.61 0.54 0.61 0.94 0.99 0.97 0.94 0.97 0.95 0.97 0.96 0.95 0.92 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.3 0.13 0.39 – 0.42 0.6 0.52 0.59 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.07 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 2 2 1 0 0 0 0 0 0 2 0 0 0 0 0
Bud 1 0 8 3 0 2 – 3 6 4 6 4 0 1 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 2 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 8 1 8 5 7 – 3 8 15 18 19 4 4 8 7 9 13 0 0 0 0 0 0
Cytoplasm 314 247 261 265 319 238 – 288 217 242 159 185 101 103 123 48 140 173 311 244 256 63 166 159
Endoplasmic Reticulum 1 1 2 1 2 2 – 2 2 3 4 9 2 0 2 2 3 5 0 0 2 1 0 1
Endosome 0 0 0 0 1 1 – 2 0 1 2 0 0 0 0 0 0 0 0 0 1 0 0 2
Golgi 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 1 1
Mitochondria 3 0 18 88 45 119 – 180 231 204 171 161 2 1 0 0 5 3 2 0 2 1 1 1
Nucleus 0 2 1 0 0 0 – 2 0 2 2 1 0 0 1 0 1 1 0 1 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 – 4 1 1 0 3 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 – 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 5 1 0 11 4 – 30 34 28 11 7 2 1 0 0 3 6 0 4 1 1 1 2
Unique Cell Count 316 253 270 289 349 302 430 383 396 292 304 107 104 127 51 144 183 320 254 271 69 176 172
Labelled Cell Count 323 263 292 366 384 374 517 501 503 375 390 112 110 135 57 162 201 320 254 271 69 176 172


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 11.3 6.1 7.2 7.7 6.4 – 6.1 5.6 6.0 5.9 5.9 10.0 9.8 10.3 12.8 12.0 12.2 8.9 10.5 10.8
Std Deviation (1e-4) 1.3 1.6 1.4 1.4 1.5 1.3 – 1.6 1.7 1.3 1.4 1.2 1.4 1.9 3.0 3.1 3.1 2.9 1.4 1.5 3.2
Intensity Change (Log2) – – – 0.23 0.33 0.07 – -0.0 -0.11 -0.02 -0.04 -0.06 0.72 0.68 0.75 1.06 0.98 1.0 0.54 0.78 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -1.6 0 0 – 0 -1.2 0 -0.7 -1.4 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.8 3.8 3.8 0 0 0 0 0 4.1
Cytoplasm -2.5 -2.7 -6.4 – -9.3 -11.4 -10.5 -11.5 -10.3 -1.0 1.3 0.1 -0.9 0.3 -1.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 1.9 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.2 2.5 9.2 – 10.1 13.9 12.1 13.0 12.0 -1.9 -2.3 -3.0 0 -1.4 -2.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 0 – 4.1 4.8 4.2 2.8 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5786 6.2885 5.7085 5.3439 4.7483 6.0572 5.0218 5.6122 5.5541 4.497 5.0328 5.5667 5.4931 7.8897 7.1173 6.9992 6.5514 7.4306
Actin 0.0994 0.0002 0.0014 0.0137 0.0328 0.0003 0.067 0.0003 0.0033 0.0237 0.0266 0.0113 0.0079 0.0008 0.0003 0.0002 0.0001 0.002
Bud 0.0012 0.001 0.0017 0.0006 0.0013 0.0001 0.0014 0.0005 0.0013 0.0031 0.0005 0.0008 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0096 0.0002 0.0008 0.0121 0.0009 0.0006 0.0322 0.0002 0.0009 0.0027 0.0005 0.0033 0.0027 0.0001 0.0002 0.0003 0.0002 0.0073
Bud Periphery 0.0015 0.0009 0.0006 0.0006 0.0016 0 0.0015 0.0001 0.0006 0.0054 0.0003 0.0008 0.0002 0 0 0 0 0
Bud Site 0.0062 0.0009 0.0022 0.0063 0.0033 0 0.0053 0.0066 0.0118 0.0128 0.0007 0.0037 0.0013 0.0003 0.0003 0.0001 0 0.0002
Cell Periphery 0.0007 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0.0002 0.0007 0.0001 0.0001 0.0001 0.0001 0 0 0 0
Cytoplasm 0.4043 0.96 0.9284 0.8448 0.7492 0.9667 0.4274 0.9692 0.9179 0.8128 0.8788 0.9005 0.772 0.9752 0.9709 0.9731 0.9878 0.9734
Cytoplasmic Foci 0.1236 0.0058 0.0164 0.0255 0.0256 0.0078 0.0612 0.0052 0.0186 0.0345 0.0224 0.0168 0.0943 0.0036 0.0059 0.007 0.0027 0.0069
Eisosomes 0.0014 0 0 0.0002 0.0001 0 0.0004 0 0 0.0011 0.0001 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0102 0.0024 0.0023 0.0035 0.0021 0.003 0.0214 0.0014 0.0019 0.0029 0.0019 0.0087 0.0127 0.0062 0.0037 0.0015 0.0009 0.0017
Endosome 0.0979 0.0059 0.0145 0.0306 0.0458 0.0072 0.0944 0.0036 0.0189 0.0292 0.0333 0.0194 0.0499 0.0019 0.0063 0.0082 0.0026 0.0032
Golgi 0.0278 0.0004 0.0025 0.007 0.0215 0.0004 0.0207 0.0003 0.0055 0.0029 0.0091 0.0105 0.0057 0.0003 0.0004 0.0004 0.0001 0.0003
Lipid Particles 0.0282 0 0.0005 0.0094 0.0235 0 0.0386 0 0.0007 0.0015 0.0042 0.001 0.0043 0 0 0 0 0
Mitochondria 0.0205 0.0002 0.0011 0.01 0.0236 0.0004 0.0114 0.0001 0.0011 0.0283 0.002 0.0122 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001
None 0.0285 0.0179 0.0168 0.0094 0.0032 0.0102 0.017 0.0097 0.0107 0.0063 0.006 0.0038 0.0188 0.0094 0.0079 0.003 0.0026 0.0025
Nuclear Periphery 0.0177 0.0005 0.0008 0.0017 0.0289 0.0005 0.0385 0.0004 0.0004 0.0019 0.0004 0.0009 0.0041 0.0004 0.0006 0.0006 0.0004 0.0003
Nucleolus 0.0024 0 0.0002 0.001 0.0008 0 0.0013 0 0.0001 0.0002 0 0 0.0002 0 0 0 0 0
Nucleus 0.0178 0.0012 0.0027 0.0027 0.0058 0.0014 0.1069 0.001 0.0008 0.0016 0.0008 0.0012 0.0036 0.0007 0.001 0.0011 0.0009 0.0009
Peroxisomes 0.0567 0.0001 0.0025 0.0082 0.0196 0.0001 0.0307 0.0001 0.0028 0.0081 0.0062 0.0028 0.0112 0 0 0 0 0.0005
Punctate Nuclear 0.0308 0.0002 0.0011 0.0044 0.0039 0.0002 0.0111 0.0002 0.0002 0.0025 0.0005 0.0004 0.0043 0.0001 0.0001 0.0001 0 0.0002
Vacuole 0.0088 0.0019 0.0028 0.0058 0.0036 0.0008 0.0074 0.001 0.002 0.0129 0.0045 0.0012 0.0046 0.0006 0.0016 0.0032 0.0011 0.0004
Vacuole Periphery 0.0046 0.0003 0.0006 0.0023 0.0025 0.0003 0.0037 0.0001 0.0004 0.0049 0.0013 0.0004 0.0007 0.0001 0.0003 0.0006 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.181 20.0134 25.4586 49.9966 35.247 35.1615 41.1938 46.8262 49.395 46.5899
Translational Efficiency 4.5516 6.4081 5.4584 1.8349 3.6841 4.2884 2.8606 2.5051 2.5804 2.8851

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
807 1768 2308 1096 1175 1675 1812 507 1982 3443 4120 1603

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 838.24 1020.42 1237.06 1433.96 924.25 1023.47 1225.91 1477.10 889.23 1021.90 1232.16 1447.60
Standard Deviation 195.80 163.86 167.28 318.33 132.05 144.05 199.43 332.04 166.53 154.55 182.20 323.35
Intensity Change Log 2 — 0.283728 0.561480 0.774570 — 0.147114 0.407498 0.676412 — 0.213702 0.482784 0.723930

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000501 0.000561 0.000141 0.003922 0.000230 0.000271 0.000160 0.001300 0.000340 0.000420 0.000149 0.003093
Bud Neck 0.004361 0.002275 0.001551 0.004553 0.002210 0.002173 0.001461 0.004526 0.003086 0.002225 0.001512 0.004544
Bud Site 0.073206 0.016375 0.006693 0.048949 0.012150 0.014132 0.010962 0.037280 0.037010 0.015284 0.008571 0.045259
Cell Periphery 0.002872 0.000060 0.000049 0.000536 0.000105 0.000062 0.000045 0.000253 0.001231 0.000061 0.000047 0.000447
Cytoplasm 0.667261* 0.781061* 0.823817* 0.695698* 0.680529* 0.725423* 0.858405* 0.694702* 0.675127* 0.753994* 0.839029* 0.695383*
Cytoplasmic Foci 0.129012 0.114631 0.021147 0.033525 0.199184* 0.132092 0.017108 0.053993 0.170613 0.123126 0.019371 0.039999
Eisosomes 0.000035 0.000017 0.000005 0.000025 0.000037 0.000021 0.000003 0.000016 0.000036 0.000019 0.000004 0.000022
Endoplasmic Reticulum 0.001104 0.000577 0.000300 0.001979 0.002025 0.000825 0.000170 0.002294 0.001650 0.000698 0.000243 0.002079
Endosome 0.009863 0.007673 0.001216 0.016864 0.010707 0.009909 0.001772 0.018690 0.010364 0.008761 0.001460 0.017441
Golgi 0.002579 0.005131 0.000146 0.013417 0.004178 0.005362 0.000508 0.010415 0.003527 0.005243 0.000305 0.012468
Lipid Particles 0.001425 0.000719 0.000521 0.006592 0.002025 0.000781 0.000450 0.004455 0.001781 0.000749 0.000490 0.005916
Mitochondria 0.002571 0.001407 0.000115 0.005752 0.003180 0.003187 0.000577 0.002658 0.002932 0.002273 0.000318 0.004774
Mitotic Spindle 0.001223 0.006532 0.006363 0.023561 0.002757 0.003061 0.014492 0.013458 0.002132 0.004843 0.009938 0.020366
None 0.015297 0.000977 0.002795 0.000879 0.000538 0.001296 0.002474 0.000605 0.006548 0.001132 0.002654 0.000793
Nuclear Periphery 0.000089 0.000053 0.000233 0.000468 0.000090 0.000087 0.000130 0.000319 0.000089 0.000070 0.000188 0.000421
Nuclear Periphery Foci 0.000700 0.000568 0.001382 0.002810 0.000935 0.000537 0.001263 0.005766 0.000839 0.000553 0.001329 0.003745
Nucleolus 0.000423 0.000622 0.000113 0.000326 0.000111 0.000170 0.000118 0.000281 0.000238 0.000402 0.000115 0.000312
Nucleus 0.038197 0.012266 0.076619 0.014833 0.017198 0.015377 0.052698 0.011928 0.025748 0.013780 0.066098 0.013914
Peroxisomes 0.001068 0.002115 0.000201 0.002364 0.000941 0.001127 0.000631 0.004738 0.000993 0.001634 0.000390 0.003115
Vacuole 0.045861 0.044322 0.056443 0.114466 0.058414 0.082017 0.035910 0.129291 0.053303 0.062661 0.047412 0.119155
Vacuole Periphery 0.002352 0.002058 0.000150 0.008479 0.002456 0.002090 0.000663 0.003032 0.002414 0.002074 0.000375 0.006756

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.26 7.20 -3.78 -3.53 -4.49 -0.87 0.37 -4.03 -3.82 -4.11 -0.82 5.24 -4.69 -4.36 -5.15
Bud Neck 4.56 6.05 0.63 -4.40 -6.13 0.14 3.06 -2.95 -3.02 -4.51 3.55 6.32 -2.54 -5.59 -7.84
Bud Site 9.96 11.89 6.24 -6.13 -10.08 -1.35 2.47 -4.33 -3.73 -5.15 8.61 11.92 1.57 -7.39 -11.09
Cell Periphery 7.48 7.49 6.56 -6.71 -6.83 7.48 8.81 -2.96 -4.49 -4.76 7.50 7.54 5.67 -7.66 -7.85
Cytoplasm -10.79 -8.40 -0.52 10.13 7.70 -4.53 -14.04 1.26 4.29 10.07 -11.16 -15.08 0.60 9.82 12.73
Cytoplasmic Foci 2.00 18.37 15.89 20.75 -6.05 10.53 33.61 24.33 16.72 -8.64 10.05 37.14 30.94 28.14 -10.37
Eisosomes 3.51 11.13 5.07 -0.08 -3.86 6.34 14.75 9.93 4.91 -6.34 5.99 18.14 8.62 1.07 -5.41
Endoplasmic Reticulum 2.03 3.57 0.01 -1.09 -1.61 2.66 4.47 1.26 -0.28 -1.20 3.33 5.24 1.28 -0.95 -2.08
Endosome 1.74 7.85 0.13 -1.45 -6.93 0.90 12.28 0.72 0.25 -4.56 2.16 14.54 0.71 -0.74 -8.25
Golgi -3.84 7.00 -2.95 -1.17 -4.79 -1.20 12.74 -0.14 0.48 -2.57 -2.97 14.52 -2.30 -0.75 -5.42
Lipid Particles 2.97 2.67 -5.64 -6.60 -6.49 2.80 2.82 -3.48 -5.99 -6.01 3.63 3.48 -6.03 -8.29 -8.21
Mitochondria 1.25 2.93 -0.27 -1.80 -3.97 0.04 2.20 1.55 1.83 -1.49 0.71 3.26 0.65 -0.10 -4.16
Mitotic Spindle -4.09 -8.41 -4.98 -2.48 -1.57 -0.22 -4.71 -1.84 -1.77 1.73 -2.68 -7.57 -4.86 -3.35 -0.46
None 7.02 6.18 7.19 1.88 13.78 -2.32 -12.24 -0.14 2.18 10.12 6.30 4.80 6.98 2.70 16.38
Nuclear Periphery 3.10 -20.26 -2.42 -2.77 1.47 0.04 -6.43 -2.37 -2.39 0.16 1.05 -16.53 -2.99 -3.26 1.07
Nuclear Periphery Foci 1.04 -8.28 -7.70 -7.97 -4.51 2.50 -2.71 -2.24 -2.52 -1.89 2.67 -6.24 -5.15 -5.75 -3.73
Nucleolus -0.72 5.24 2.23 1.12 -3.11 -1.48 -2.72 -2.52 0.28 -1.46 -1.16 3.96 -0.35 1.08 -3.43
Nucleus 8.15 -14.92 5.37 -5.43 29.57 1.27 -22.58 0.30 -0.81 22.01 7.80 -26.89 3.79 -4.92 35.33
Peroxisomes -2.53 4.10 -1.34 1.34 -5.08 -1.50 3.00 -2.27 -1.99 -2.90 -3.01 5.91 -2.80 -0.52 -5.32
Vacuole 0.27 -13.00 -18.62 -19.72 -10.23 -6.50 -6.87 -14.05 -11.08 -10.96 -4.16 -14.31 -23.16 -21.03 -15.09
Vacuole Periphery 0.79 7.12 -2.05 -2.42 -4.60 0.79 6.03 2.14 1.25 -2.08 1.15 9.43 -1.44 -1.97 -4.88
Standard name
Human Ortholog
Description Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Yke2

Yke2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yke2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available