Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0.06 0 0.07 0.06 0 0 0 0 0.06 0 0.05 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.82 0.83 0.84 0.81 0.67 0.64 0.6 0.6 0.53 0.57 0.39 0.51 0.8 0.8 0.79 – – 0.85 0.55 0.57 0.48 0.83 0.82 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0.07 0.07 0.1 – – 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.12 0.12 0.08 0.11 0.12 0.37 0.43 0.41 0.59 0.58 0.7 0.66 0 0 0 – – 0 0.1 0.1 0.21 0.05 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.16 0.08 0.11 0.08 0.09 0 0.08 0 0.07 0 0 – – 0.05 0.14 0.14 0.15 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.09 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 0 0 0 0 0 0 0 0 0 2 2 0 – – 1 11 4 2 2 7 12
Bud 22 4 11 15 12 13 15 31 23 9 15 11 7 6 4 – – 1 10 5 2 2 10 11
Bud Neck 7 0 4 8 9 4 5 15 4 2 0 0 0 1 0 – – 0 11 7 5 0 3 6
Bud Site 1 0 1 1 0 4 9 5 5 0 4 0 2 1 2 – – 0 – – – – – –
Cell Periphery 2 0 0 3 1 1 0 0 1 1 1 2 0 0 2 – – 2 1 0 1 0 5 6
Cytoplasm 297 136 142 187 193 229 308 438 201 216 109 169 354 549 596 – – 138 233 106 90 161 470 421
Endoplasmic Reticulum 1 2 2 0 1 0 0 3 0 0 0 0 1 2 1 – – 11 5 0 2 1 1 2
Endosome 7 1 1 3 21 9 4 5 3 1 2 0 32 50 77 – – 9 14 3 7 1 7 1
Golgi 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 – – 1 2 2 0 1 2 1
Mitochondria 45 20 14 26 34 132 220 297 222 219 194 219 5 7 6 – – 6 40 18 40 10 37 44
Nucleus 0 0 1 1 0 1 1 7 5 3 1 1 3 1 7 – – 1 0 1 0 0 0 0
Nuclear Periphery 1 1 1 0 0 0 1 1 1 0 2 0 0 0 0 – – 0 0 0 0 0 0 1
Nucleolus 2 1 0 2 0 6 7 6 0 6 1 2 0 2 0 – – 0 1 0 0 0 0 0
Peroxisomes 0 0 0 2 6 7 4 2 6 1 1 0 12 25 30 – – 0 2 0 1 0 0 0
SpindlePole 9 4 7 16 45 27 57 58 35 13 22 9 30 25 29 – – 8 61 25 27 4 10 11
Vac/Vac Membrane 3 3 2 3 10 5 13 6 4 6 0 2 35 66 67 – – 7 11 4 2 2 4 18
Unique Cell Count 362 163 169 232 288 359 514 724 377 376 276 331 445 690 751 162 424 188 189 194 576 553
Labelled Cell Count 397 173 189 267 332 439 645 874 510 477 352 415 483 737 821 185 424 188 189 194 576 553


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.5 3.5 3.6 3.6 3.1 2.6 2.9 2.9 2.6 2.7 2.4 4.3 4.1 4.3 4.4 4.7 5.0 3.8 3.7 4.5
Std Deviation (1e-4) 0.6 0.6 0.6 1.5 1.3 0.9 1.0 1.1 1.3 1.1 1.2 1.1 1.3 1.0 1.3 1.6 1.2 0.9 1.4 1.2 1.7
Intensity Change (Log2) – – – 0.05 0.07 -0.17 -0.4 -0.23 -0.27 -0.41 -0.33 -0.51 0.3 0.26 0.3 0.36 0.45 0.54 0.15 0.11 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.1637 -0.9525 -0.812 -1.1506 -1.1536 -1.2994 -0.522 0.0998 0.3491 0.1895 -0.5312 -0.1848 1.4712 1.9557 2.0067 2.3354 1.715 1.6434
Actin 0.0403 0.0005 0.0182 0.0005 0.0505 0.0096 0.02 0.0004 0.0193 0.0183 0.0001 0.0059 0.0249 0.0007 0.0036 0.002 0.0004 0.0057
Bud 0.0019 0.0066 0.0028 0 0.0002 0.0014 0.0003 0.0031 0.0011 0.0001 0.0003 0.0002 0.0091 0.0336 0.0106 0.0152 0.0153 0.0042
Bud Neck 0.0062 0.0007 0.0529 0.0782 0.002 0.0026 0.001 0.0002 0.0763 0.0243 0 0.0024 0.0014 0.0036 0.0615 0.0827 0.0007 0.0063
Bud Periphery 0.0034 0.0038 0.0016 0 0.0002 0.0013 0.0005 0.0018 0.0031 0.0002 0.0011 0.0015 0.0048 0.0045 0.0032 0.0048 0.0046 0.0035
Bud Site 0.0166 0.0235 0.0064 0.0001 0.0039 0.0007 0.0035 0.0055 0.0121 0.0006 0.0001 0.0001 0.0112 0.032 0.014 0.0028 0.0032 0.003
Cell Periphery 0.0003 0.0007 0.0004 0 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001 0 0.0001 0.0004 0.0012 0.0008 0.0003 0.0005 0.0002
Cytoplasm 0.0252 0.0586 0.0547 0.0666 0.0294 0.0636 0.0097 0.0309 0.0278 0.0365 0.0102 0.0199 0.0251 0.0927 0.0556 0.0492 0.0598 0.0514
Cytoplasmic Foci 0.0924 0.0814 0.3125 0.2588 0.0697 0.0512 0.09 0.1021 0.2238 0.0634 0.0041 0.0165 0.1256 0.0302 0.272 0.2088 0.0099 0.0387
Eisosomes 0.001 0.0002 0.0005 0.0001 0.0015 0.0002 0.0007 0.0002 0.0012 0.0006 0.0002 0.0002 0.0008 0.0002 0.0002 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0022 0.0007 0.0018 0.0027 0.0027 0.0007 0.0005 0.0005 0.001 0.0007 0.0002 0.0005 0.0026 0.0016 0.0022 0.0009 0.0009 0.0004
Endosome 0.0133 0.0035 0.0097 0.0252 0.0027 0.0013 0.0037 0.0155 0.0049 0.004 0.0002 0.0037 0.0042 0.0022 0.0045 0.0042 0.0011 0.0009
Golgi 0.0054 0.0007 0.0036 0.0017 0.002 0.0003 0.0022 0.0004 0.002 0.0011 0.0001 0.0026 0.0018 0.0002 0.001 0.0014 0.0001 0.0007
Lipid Particles 0.0104 0.0129 0.0159 0.0118 0.0033 0.0017 0.0084 0.001 0.0063 0.0023 0.0002 0.0059 0.0038 0.0005 0.0032 0.002 0.0003 0.0014
Mitochondria 0.0024 0.0008 0.0023 0.0005 0.0005 0.0005 0.0009 0.0003 0.0064 0.0009 0.0004 0.0017 0.0028 0.0012 0.0022 0.0056 0.0012 0.0013
None 0.7356 0.7708 0.4369 0.4923 0.8194 0.8471 0.8311 0.8184 0.5538 0.8181 0.9815 0.9295 0.733 0.733 0.4254 0.389 0.8816 0.8303
Nuclear Periphery 0.0044 0.0007 0.0028 0.0015 0.0019 0.0007 0.0006 0.0003 0.0013 0.0006 0.0001 0.0018 0.0052 0.0024 0.0028 0.0045 0.0019 0.0009
Nucleolus 0.0017 0.0008 0.0019 0.0005 0.0004 0.0005 0.0005 0.0005 0.0012 0.0005 0.0001 0.0002 0.0017 0.0028 0.0043 0.0083 0.0017 0.0019
Nucleus 0.0019 0.0036 0.005 0.0034 0.0007 0.0023 0.0003 0.0053 0.0024 0.0008 0.0002 0.0004 0.0039 0.0147 0.0192 0.0236 0.0081 0.0075
Peroxisomes 0.0081 0.0043 0.0192 0.0161 0.0045 0.0027 0.0172 0.0056 0.0213 0.0133 0.0004 0.0029 0.0079 0.0007 0.0173 0.0132 0.0003 0.0056
Punctate Nuclear 0.0255 0.0241 0.0497 0.0394 0.0037 0.011 0.0082 0.0067 0.0334 0.0132 0.0003 0.0034 0.0283 0.0397 0.0948 0.1799 0.0069 0.0353
Vacuole 0.0012 0.0009 0.0009 0.0005 0.0003 0.0004 0.0003 0.0013 0.0007 0.0003 0.0002 0.0003 0.001 0.002 0.0012 0.0009 0.0013 0.0005
Vacuole Periphery 0.0008 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0004 0.0003 0.0003 0.0005 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.7491 22.1097 8.997 6.0927 16.5508 12.7583 12.9804 13.3847 8.511 15.4397
Translational Efficiency 1.0983 0.7688 0.6765 0.8197 0.7848 1.2042 1.2322 0.7119 0.7653 0.694

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1864 1377 133 1544 939 2042 2224 836 2803 3419 2357 2380

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.86 670.04 874.81 789.36 670.71 685.92 842.09 764.18 626.25 679.52 843.94 780.52
Standard Deviation 67.35 95.89 79.20 105.07 77.48 96.57 92.63 181.54 77.61 96.61 92.24 137.41
Intensity Change Log 2 — 0.150033 0.534756 0.386469 — 0.032351 0.328285 0.188223 — 0.089304 0.429795 0.285549

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000341 0.000780 0.002102 0.000078 0.000360 0.000296 0.001150 0.000057 0.000352 0.000323 0.001767
Bud Neck 0.024014 0.037715 0.004742 0.020865 0.011017 0.048560 0.013947 0.014196 0.019660 0.044192 0.013428 0.018523
Bud Site 0.004344 0.018582 0.027103 0.055457 0.002680 0.019456 0.013162 0.056049 0.003786 0.019104 0.013949 0.055665
Cell Periphery 0.000266 0.000140 0.000358 0.000416 0.000138 0.000167 0.000102 0.002480 0.000223 0.000156 0.000116 0.001141
Cytoplasm 0.497114* 0.392983* 0.172262* 0.359290* 0.571468* 0.382834* 0.542614* 0.417665* 0.522023* 0.386922* 0.521716* 0.379795*
Cytoplasmic Foci 0.157722 0.274356* 0.024995 0.035896 0.132978 0.239484* 0.029117 0.060769 0.149433 0.253529* 0.028885 0.044633
Eisosomes 0.000131 0.000132 0.000131 0.000089 0.000065 0.000125 0.000039 0.000141 0.000109 0.000127 0.000044 0.000107
Endoplasmic Reticulum 0.000371 0.000695 0.005329 0.003257 0.000260 0.000420 0.001134 0.000878 0.000333 0.000531 0.001371 0.002421
Endosome 0.003556 0.024711 0.007318 0.018295 0.003143 0.021795 0.003808 0.009470 0.003418 0.022970 0.004006 0.015195
Golgi 0.000723 0.006617 0.000172 0.006643 0.000761 0.005630 0.000376 0.003303 0.000736 0.006027 0.000364 0.005470
Lipid Particles 0.003968 0.009252 0.006302 0.002125 0.003288 0.005963 0.001153 0.003964 0.003740 0.007288 0.001443 0.002771
Mitochondria 0.001886 0.013260 0.000649 0.007306 0.000959 0.014688 0.000741 0.006959 0.001576 0.014113 0.000736 0.007184
Mitotic Spindle 0.001010 0.012786 0.001676 0.059800 0.000445 0.018690 0.010550 0.027549 0.000821 0.016312 0.010050 0.048472
None 0.018842 0.009269 0.004934 0.004483 0.010596 0.003849 0.004549 0.029139 0.016080 0.006032 0.004570 0.013144
Nuclear Periphery 0.000275 0.000514 0.002289 0.002411 0.000258 0.000427 0.001291 0.000893 0.000269 0.000462 0.001347 0.001878
Nuclear Periphery Foci 0.000220 0.001243 0.003149 0.002666 0.000419 0.000306 0.000814 0.002488 0.000286 0.000683 0.000946 0.002603
Nucleolus 0.003710 0.005540 0.001360 0.001408 0.002023 0.003125 0.000792 0.003655 0.003145 0.004098 0.000824 0.002197
Nucleus 0.265065* 0.116555 0.667417* 0.303012* 0.243227* 0.147905* 0.321266* 0.298661* 0.257749* 0.135279* 0.340799* 0.301484*
Peroxisomes 0.006748 0.029676 0.005029 0.019135 0.005093 0.038586 0.007991 0.015468 0.006193 0.034997 0.007824 0.017847
Vacuole 0.009710 0.043504 0.063654 0.089193 0.010832 0.046058 0.046081 0.040538 0.010086 0.045029 0.047073 0.072102
Vacuole Periphery 0.000277 0.002130 0.000352 0.006151 0.000275 0.001573 0.000177 0.004586 0.000276 0.001797 0.000187 0.005602

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.03 -6.08 -5.95 -5.12 -2.56 -7.41 -9.03 -6.50 -5.12 -4.61 -11.53 -10.86 -7.58 -6.39 -5.76
Bud Neck -3.95 2.65 -0.88 3.57 -3.63 -12.28 -4.42 -1.92 11.84 2.88 -10.37 -1.09 -1.54 10.20 -0.40
Bud Site -8.38 -2.87 -13.41 -7.83 -1.17 -11.11 -11.25 -13.49 -9.64 -9.96 -13.13 -10.56 -18.88 -12.17 -11.68
Cell Periphery 2.35 -0.65 0.00 -2.36 0.66 -0.49 3.57 -12.00 -11.71 -12.18 1.73 2.81 -9.34 -10.78 -11.52
Cytoplasm 10.26 23.18 24.18 11.06 -8.62 16.24 9.55 12.53 -1.56 5.15 18.43 9.77 26.21 8.66 14.33
Cytoplasmic Foci -13.06 17.90 23.49 35.31 -0.93 -12.10 15.41 10.70 27.49 -4.94 -16.95 26.35 25.20 46.89 -2.08
Eisosomes 0.09 -2.78 1.53 1.20 3.24 -9.08 2.54 -6.76 -1.04 -8.20 -3.02 10.44 -2.34 0.25 -10.34
Endoplasmic Reticulum -2.82 -9.11 -20.58 -20.52 3.97 -3.88 -17.15 -6.84 -5.65 4.95 -2.68 -15.53 -20.94 -21.36 -7.75
Endosome -14.17 -4.15 -6.03 8.75 1.08 -13.94 -1.97 -3.57 7.56 -2.77 -19.50 -3.07 -6.98 11.37 -4.92
Golgi -10.23 3.15 -2.53 5.16 -3.36 -9.12 1.68 -1.94 3.35 -2.69 -14.22 2.84 -3.20 6.01 -4.22
Lipid Particles -6.74 -2.94 1.90 7.86 3.41 -5.07 5.29 0.20 2.61 -1.70 -8.23 7.03 1.17 7.85 -3.42
Mitochondria -9.13 2.62 -1.96 7.40 -4.17 -12.09 1.01 -4.34 5.46 -4.48 -14.34 2.60 -4.20 9.76 -6.02
Mitotic Spindle -6.63 -2.22 -13.27 -9.16 -7.04 -8.75 -8.28 -8.01 -2.70 -3.49 -11.07 -8.88 -15.39 -8.98 -8.96
None 4.65 7.38 8.53 4.57 -0.73 7.62 7.20 -4.30 -6.94 -6.70 8.20 10.39 3.36 -4.48 -6.67
Nuclear Periphery -5.07 -11.22 -14.58 -13.33 -1.96 -7.16 -27.46 -9.07 -7.33 6.38 -8.24 -29.38 -16.11 -14.74 -4.64
Nuclear Periphery Foci -3.59 -3.54 -7.51 -4.33 2.06 1.32 -0.49 -1.11 -2.73 -1.02 -0.92 -4.55 -6.24 -6.12 -3.19
Nucleolus -2.00 -0.42 1.45 3.56 0.91 0.09 1.04 0.11 0.04 -0.97 -0.84 1.87 1.26 2.42 -0.89
Nucleus 21.17 -11.55 -8.96 -25.54 8.51 10.49 -10.30 -4.90 -14.63 3.64 22.26 -13.56 -10.09 -28.41 2.95
Peroxisomes -11.69 -0.77 -4.19 6.40 -1.35 -14.87 -2.23 -2.77 8.06 -1.66 -18.77 -1.90 -4.98 10.88 -3.22
Vacuole -17.12 -7.47 -28.15 -20.45 -3.06 -18.64 -23.77 -11.20 -3.05 4.30 -25.88 -25.66 -29.34 -19.36 -8.17
Vacuole Periphery -9.27 -1.21 -3.37 -0.08 -3.17 -5.70 4.06 -3.17 -1.86 -3.28 -9.55 3.07 -4.63 -1.60 -4.84
Standard name
Human Ortholog
Description B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (37%), nucleus (1%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Clb4

Clb4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Clb4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available