Standard name
Human Ortholog
Description Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.75 0.95 0.79 0.86 0.89 0.86 0.89 0.84 0.84 0.72 0.76 0.96 0.92 0.92 0.71 0.49 0.51 0.37 0.5 0.56 0.67 0.57 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.15 0.22 0.11 0.09 0.09 0 0.06 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.29 0 0 0 0.05 0.07 0.05 0.3 0.17 0.42 0.26 0 0 0 0.06 0.08 0 0.09 0.12 0.17 0.07 0.06 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.18 0.1 0 0.06 0.06 0 0.07 0 0.08 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.36 0.34 0.29 0.24 0.1 0.14 0.18 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 1 0 3 3
Bud 0 0 0 3 1 3 5 2 5 8 8 4 0 2 0 0 1 0 0 0 0 2 8 8
Bud Neck 0 0 3 5 6 9 14 21 3 10 2 10 7 7 2 0 0 0 1 0 1 0 1 1
Bud Site 0 0 0 1 0 0 1 0 0 0 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 0 0 1 1 2 0 0 1 3 0 3 2 0 0 1 0 0 0 0 0 0
Cytoplasm 105 80 174 136 260 280 323 374 176 306 160 339 158 317 375 44 26 49 62 64 107 105 214 206
Endoplasmic Reticulum 0 4 5 0 0 1 0 0 0 0 0 0 0 3 1 4 1 7 3 0 1 0 3 3
Endosome 1 0 2 3 7 4 11 3 0 3 2 7 2 4 17 7 8 21 18 11 18 6 23 45
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 0 0 0 0 2
Mitochondria 6 31 0 2 1 16 28 21 63 64 93 117 1 0 0 4 4 1 14 15 33 11 20 31
Nucleus 1 1 0 1 0 4 2 4 2 3 1 1 1 0 0 1 1 1 0 1 0 1 2 3
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 4 0 1 0 2 2
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 2 0 0 3 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 31 30 12 22 26 5 25 8 35 3 12 12 0 3 7 3 0 1 1 12 15
Vac/Vac Membrane 12 4 1 1 4 5 5 4 1 1 2 4 1 3 12 15 19 33 47 30 19 21 68 97
Unique Cell Count 124 106 184 173 302 313 376 418 209 366 222 445 165 344 406 62 53 97 166 128 193 158 374 432
Labelled Cell Count 128 120 190 183 312 335 413 460 255 421 277 526 173 354 425 76 64 121 166 128 193 158 374 432


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.2 5.1 4.8 5.2 4.2 4.0 4.3 3.5 3.6 3.6 3.7 4.7 4.7 4.7 5.1 6.1 6.7 5.3 5.6 5.7
Std Deviation (1e-4) 0.7 0.4 0.9 1.5 1.4 1.4 1.2 1.4 1.4 1.1 1.3 1.4 1.0 0.9 1.1 1.5 1.5 1.6 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.07 0.04 -0.28 -0.34 -0.22 -0.54 -0.49 -0.51 -0.44 -0.11 -0.1 -0.1 0.01 0.28 0.4 0.05 0.16 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 8.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0351 -0.2966 -0.7802 -0.8835 -1.0584 -0.5498 2.028 2.8461 2.4641 2.1737 2.2825 2.7357 -0.2491 0.2644 -0.0494 -0.1865 0.0242 -0.1434
Actin 0.0024 0.0005 0.0004 0.0005 0.0005 0.0001 0.0089 0.0001 0.0008 0.0029 0.0262 0.0003 0.0229 0.0001 0.0053 0.0001 0.0207 0.0025
Bud 0.001 0.0001 0.004 0.0001 0.0005 0.0002 0.0005 0.0005 0.002 0.0001 0.0002 0.0002 0.0006 0.0002 0.0056 0.0002 0.0078 0.0018
Bud Neck 0.0002 0.0002 0.0003 0.0003 0.0001 0.0003 0.0017 0.0002 0.0002 0.0002 0.0145 0.0004 0.007 0.0001 0.0012 0.0001 0.0014 0.0022
Bud Periphery 0.0004 0.0001 0.0011 0.0001 0.0006 0.0002 0.0014 0.0008 0.0026 0.0002 0.0003 0.0014 0.0015 0.0005 0.0042 0.0003 0.0142 0.0012
Bud Site 0.001 0.0009 0.0018 0.0001 0.0001 0 0.0031 0.0051 0.0022 0.0002 0.017 0 0.0024 0.0008 0.0039 0.0001 0.0025 0.0006
Cell Periphery 0.0001 0.0001 0.0002 0 0 0 0.0005 0.0002 0.0002 0.0001 0.0003 0.0002 0.0004 0.0002 0.0003 0.0002 0.0001 0.0002
Cytoplasm 0.2079 0.3086 0.2425 0.291 0.3572 0.3751 0.142 0.1878 0.142 0.1582 0.2161 0.2398 0.1771 0.3052 0.2376 0.2695 0.2494 0.307
Cytoplasmic Foci 0.0762 0.0628 0.0785 0.0586 0.0388 0.0707 0.0584 0.0521 0.0638 0.0973 0.0769 0.0567 0.0434 0.0548 0.0376 0.0372 0.0335 0.0415
Eisosomes 0.0001 0.0001 0.0001 0.0002 0 0 0.0005 0.0001 0.0003 0.0003 0.0004 0.0001 0.0007 0.0001 0.0001 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0068 0.0072 0.0028 0.0041 0.0033 0.0022 0.003 0.0008 0.0015 0.003 0.0021 0.0022 0.0047 0.0027 0.0038 0.0028 0.0037 0.0033
Endosome 0.0562 0.0652 0.0527 0.1175 0.0495 0.0361 0.042 0.0246 0.0402 0.0613 0.0637 0.0429 0.0359 0.0556 0.0282 0.0381 0.0449 0.0378
Golgi 0.0017 0.0018 0.0019 0.0011 0.0004 0.0006 0.0033 0.0005 0.0012 0.0023 0.006 0.0008 0.0032 0.0008 0.0022 0.0008 0.0028 0.0015
Lipid Particles 0.009 0.0116 0.007 0.0039 0.0002 0.0034 0.0119 0.0007 0.0051 0.0342 0.0145 0.003 0.0047 0.001 0.0035 0.0089 0.0044 0.002
Mitochondria 0.0007 0.0014 0.001 0.0018 0.0004 0.0008 0.0206 0.0003 0.0022 0.004 0.0018 0.0015 0.0027 0.001 0.0012 0.0081 0.0016 0.0045
None 0.6133 0.5167 0.5798 0.4806 0.53 0.4985 0.6604 0.7153 0.7225 0.6118 0.5205 0.6413 0.6637 0.5611 0.6451 0.6078 0.5837 0.5768
Nuclear Periphery 0.0041 0.0042 0.0027 0.0055 0.0014 0.0015 0.0082 0.0006 0.0008 0.0029 0.0019 0.0008 0.0028 0.0009 0.0019 0.0025 0.0081 0.0011
Nucleolus 0.0006 0.0002 0.0005 0.0005 0.0001 0.0002 0.0028 0.0003 0.0004 0.0006 0.0003 0.0001 0.003 0.0002 0.0003 0.0004 0.0006 0.0006
Nucleus 0.003 0.0019 0.0048 0.0022 0.0012 0.0015 0.0079 0.0008 0.001 0.0014 0.0008 0.001 0.0036 0.0012 0.0018 0.0012 0.0041 0.0011
Peroxisomes 0.0014 0.0011 0.0029 0.0009 0.0002 0.0007 0.0061 0.0003 0.0019 0.0101 0.0169 0.0006 0.0029 0.001 0.0057 0.0012 0.0019 0.0006
Punctate Nuclear 0.007 0.0016 0.0036 0.0044 0.001 0.0016 0.0048 0.0008 0.0029 0.0031 0.0022 0.0012 0.0049 0.0008 0.0011 0.0011 0.0031 0.002
Vacuole 0.006 0.0112 0.0101 0.0208 0.0122 0.0053 0.0104 0.0076 0.0051 0.0046 0.0153 0.0048 0.0106 0.0106 0.008 0.0156 0.0088 0.0097
Vacuole Periphery 0.0008 0.0025 0.0014 0.006 0.0023 0.001 0.0015 0.0006 0.001 0.0012 0.0023 0.0009 0.0013 0.0011 0.0015 0.0037 0.0022 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1613 1024 2252 1713 1539 10 147 1457 3152 1034 2399 3170

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 563.06 711.67 761.28 793.07 581.88 369.60 895.69 779.77 572.25 708.36 769.52 786.96
Standard Deviation 62.00 86.56 78.73 102.86 65.74 5.49 96.65 115.56 64.54 92.41 86.20 109.08
Intensity Change Log 2 — 0.337920 0.435139 0.494160 — -0.654757 0.622278 0.422327 — -0.082545 0.533275 0.458100

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000260 0.000384 0.000104 0.000628 0.000073 0.000064 0.000382 0.000182 0.000169 0.000381 0.000121 0.000423
Bud Neck 0.035640 0.032269 0.006177 0.016853 0.009955 0.000006 0.001702 0.004559 0.023099 0.031957 0.005903 0.011202
Bud Site 0.006006 0.016715 0.010370 0.039757 0.004190 0.000013 0.002371 0.024870 0.005120 0.016554 0.009880 0.032915
Cell Periphery 0.000373 0.000109 0.000083 0.000172 0.000117 0.000038 0.000261 0.000205 0.000248 0.000108 0.000094 0.000187
Cytoplasm 0.351061* 0.341738* 0.521497* 0.483239* 0.497542* 0.000027 0.254745* 0.574408* 0.422582* 0.338433* 0.505151* 0.525143*
Cytoplasmic Foci 0.343497* 0.320310* 0.021373 0.021776 0.209878* 0.000033 0.036989 0.028094 0.278256* 0.317212* 0.022330 0.024680
Eisosomes 0.000676 0.000119 0.000027 0.000041 0.000093 0.000090 0.000149 0.000041 0.000391 0.000119 0.000035 0.000041
Endoplasmic Reticulum 0.002212 0.001311 0.001397 0.001309 0.001117 0.000001 0.006247 0.001016 0.001677 0.001298 0.001694 0.001174
Endosome 0.013083 0.054153 0.003734 0.006337 0.010710 0.000001 0.008855 0.003344 0.011924 0.053629 0.004048 0.004962
Golgi 0.012384 0.009805 0.000237 0.003827 0.002143 0.000004 0.000122 0.001089 0.007384 0.009710 0.000230 0.002568
Lipid Particles 0.033601 0.008964 0.001153 0.001028 0.008906 0.000034 0.006602 0.001199 0.021543 0.008878 0.001487 0.001106
Mitochondria 0.037375 0.012094 0.000680 0.006331 0.008020 0.000006 0.000311 0.000685 0.023042 0.011977 0.000658 0.003736
Mitotic Spindle 0.000194 0.004549 0.009371 0.050995 0.000473 0.000382 0.000224 0.010585 0.000330 0.004508 0.008811 0.032422
None 0.006709 0.002485 0.002770 0.003419 0.008350 0.998992* 0.004114 0.005460 0.007510 0.012122 0.002853 0.004357
Nuclear Periphery 0.000496 0.000747 0.002122 0.001646 0.000483 0.000000 0.002115 0.001059 0.000490 0.000739 0.002122 0.001376
Nuclear Periphery Foci 0.000345 0.001046 0.000544 0.000536 0.000426 0.000046 0.004899 0.000929 0.000384 0.001036 0.000811 0.000717
Nucleolus 0.001693 0.002013 0.000543 0.000695 0.002262 0.000027 0.001077 0.000737 0.001971 0.001993 0.000576 0.000715
Nucleus 0.110760 0.056939 0.356789* 0.257453* 0.188217* 0.000009 0.502285* 0.254275* 0.148579* 0.056388 0.365704* 0.255993*
Peroxisomes 0.017904 0.020030 0.000699 0.001248 0.004273 0.000228 0.000870 0.000428 0.011249 0.019839 0.000709 0.000871
Vacuole 0.023194 0.109148 0.060110 0.099902 0.040846 0.000001 0.165391 0.085517 0.031813 0.108092 0.066561 0.093290
Vacuole Periphery 0.002539 0.005074 0.000219 0.002807 0.001926 0.000000 0.000289 0.001318 0.002240 0.005025 0.000223 0.002123

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.12 3.91 -1.55 -0.01 -3.40 NAN NAN NAN NAN NAN -4.17 0.77 -3.19 1.11 -3.68
Bud Neck 1.13 12.05 9.32 7.71 -3.93 NAN NAN NAN NAN NAN -3.26 11.62 10.23 9.63 -1.96
Bud Site -6.40 -2.18 -6.94 -2.01 -5.29 NAN NAN NAN NAN NAN -7.11 -2.90 -9.42 -2.03 -6.44
Cell Periphery 12.00 13.82 11.53 -0.49 -3.78 NAN NAN NAN NAN NAN 10.68 13.38 7.91 -2.97 -5.54
Cytoplasm 0.88 -13.51 -5.55 -5.46 7.07 NAN NAN NAN NAN NAN 8.49 -3.88 -3.69 -10.92 0.47
Cytoplasmic Foci 2.74 56.95 58.58 41.53 3.18 NAN NAN NAN NAN NAN -4.53 61.74 63.67 40.64 0.93
Eisosomes 14.13 16.48 16.12 11.96 -6.63 NAN NAN NAN NAN NAN 12.72 16.92 16.60 11.95 -3.52
Endoplasmic Reticulum 3.02 0.41 1.06 -3.61 1.41 NAN NAN NAN NAN NAN 1.87 -4.34 -0.51 -3.03 5.84
Endosome -13.94 9.75 10.85 17.59 1.02 NAN NAN NAN NAN NAN -14.46 9.86 13.46 17.77 2.18
Golgi 2.45 14.65 11.14 8.55 -2.62 NAN NAN NAN NAN NAN -2.33 15.85 11.51 9.93 -3.13
Lipid Particles 14.07 21.56 21.68 8.35 1.93 NAN NAN NAN NAN NAN 10.26 23.53 24.10 8.27 4.57
Mitochondria 9.06 15.37 13.93 5.33 -3.92 NAN NAN NAN NAN NAN 5.59 16.34 15.01 6.35 -3.74
Mitotic Spindle -3.63 -7.07 -9.80 -7.74 -6.35 NAN NAN NAN NAN NAN -3.52 -6.97 -11.36 -7.48 -5.67
None 3.51 4.31 3.23 -1.47 -3.64 NAN NAN NAN NAN NAN -1.41 7.28 5.09 2.55 -4.92
Nuclear Periphery -0.78 -21.86 -17.74 -7.22 -0.72 NAN NAN NAN NAN NAN -0.80 -26.16 -21.82 -5.42 4.79
Nuclear Periphery Foci -2.80 -3.68 -2.23 2.10 2.63 NAN NAN NAN NAN NAN -2.73 -5.70 -6.22 0.97 2.61
Nucleolus -0.52 2.88 3.76 2.83 1.47 NAN NAN NAN NAN NAN -0.05 4.05 5.21 2.82 1.70
Nucleus 11.86 -39.40 -28.24 -36.98 7.95 NAN NAN NAN NAN NAN 21.78 -36.16 -27.22 -45.20 12.32
Peroxisomes -0.97 14.55 14.63 12.09 0.68 NAN NAN NAN NAN NAN -4.92 15.81 16.37 12.19 1.66
Vacuole -19.70 -19.83 -28.31 -10.09 -14.80 NAN NAN NAN NAN NAN -17.56 -18.92 -33.22 -9.41 -13.30
Vacuole Periphery -4.22 13.11 3.71 5.98 -3.18 NAN NAN NAN NAN NAN -4.51 8.24 3.67 6.68 -3.97
Standard name
Human Ortholog
Description Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy
Localization
Cell Percentages cytoplasm (18%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg38

Atg38


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg38-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available