Standard name
Human Ortholog
Description Assembly factor for the eIF2 translation initiation factor complex; regulates translational initiation; conserved residues of this ATP-Grasp protein that bind to ATP-Mg2+ in the pombe ortholog are required for complex assembly in budding yeast; interaction with eIF2 subunit Gcd11p facilitates complex assembly and activity; required for the START transition and timely progression through G2; regulated by nutrient availability; human ortholog complements the yeast mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.95 1.0 1.0 0.99 0.99 0.94 0.89 0.89 0.84 0.83 0.76 0.78 1.0 0.99 1.0 0.96 0.94 0.98 0.88 0.96 0.91 0.95 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.11 0.17 0.17 0.25 0.26 0.43 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 0 0 1 2 1 1 1 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud 2 0 0 1 0 2 6 15 12 8 21 14 3 0 1 0 0 0 0 0 0 0 3 10
Bud Neck 0 0 0 0 1 1 2 0 1 2 0 2 1 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 13 16 11 2 9 10 2 6 8 8 7 15 15 12 16 0 0 0 0 0 0
Cytoplasm 79 112 80 336 542 430 518 460 353 392 288 270 429 476 673 149 138 175 87 110 107 251 518 496
Endoplasmic Reticulum 1 0 0 0 0 1 2 0 1 0 1 2 0 1 0 6 3 8 1 0 0 2 5 6
Endosome 0 0 0 0 1 2 2 0 2 1 0 0 1 0 0 0 0 1 1 0 1 1 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Mitochondria 4 2 0 4 15 52 99 88 105 122 161 115 1 1 0 2 2 0 1 0 2 2 5 2
Nucleus 1 2 0 0 1 0 2 1 5 6 1 4 0 1 1 0 3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 0 1 2 0 3 2 0 0 0 0 1 0 0 0 0 0 0 1 5
Vac/Vac Membrane 0 0 0 2 2 3 14 8 6 11 2 6 0 1 2 5 2 1 0 2 1 3 4 5
Unique Cell Count 83 112 80 338 548 459 582 514 422 473 377 347 431 479 675 156 147 178 99 115 118 265 554 545
Labelled Cell Count 88 116 82 359 578 502 650 586 496 549 483 425 444 487 693 179 161 201 99 115 118 265 554 545


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.1 6.8 6.8 6.6 5.2 4.8 5.1 4.5 4.6 4.2 4.0 7.0 7.2 7.2 10.9 10.7 11.1 6.9 7.1 7.5
Std Deviation (1e-4) 0.9 0.8 1.1 1.4 1.7 1.2 1.3 1.5 1.2 1.4 1.2 1.0 1.0 1.1 1.4 2.0 1.9 2.1 1.2 1.2 1.5
Intensity Change (Log2) – – – 0.01 -0.04 -0.39 -0.49 -0.43 -0.6 -0.57 -0.7 -0.77 0.04 0.08 0.08 0.68 0.65 0.71 0.03 0.06 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 2.3
Cytoplasm -0.7 -0.9 -2.3 -3.1 -3.0 -3.9 -4.0 -4.8 -4.7 -0.6 -0.7 -0.5 -1.9 -2.3 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 0 4.0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7152 3.6681 3.1572 3.2056 2.7241 3.0478 1.1144 2.1647 1.955 1.3194 1.5545 1.8896 1.7319 1.6938 1.1351 1.3607 0.939 1.1809
Actin 0.0185 0.0043 0.0133 0.0015 0.0231 0.0036 0.0366 0.0002 0.0265 0.017 0.0064 0.0053 0.0748 0.0001 0.0159 0.0001 0.0647 0.0011
Bud 0.0011 0.0012 0.001 0.0007 0.0004 0.0014 0.0062 0.0004 0.0007 0.0013 0.0006 0.0001 0.0017 0.0001 0.0022 0.0002 0.0005 0.0005
Bud Neck 0.0005 0.0004 0.0004 0.0002 0.0009 0.0007 0.0106 0.0001 0.0013 0.0008 0.0011 0.0007 0.0031 0.0001 0.0004 0.0002 0.0006 0.0005
Bud Periphery 0.0017 0.0012 0.0009 0.0012 0.0005 0.0011 0.0031 0.0003 0.0007 0.0021 0.0007 0.0001 0.0017 0 0.0029 0.0001 0.0006 0.0005
Bud Site 0.0039 0.0025 0.0041 0.0004 0.0011 0.0002 0.0061 0.0014 0.0062 0.0014 0.0011 0.0003 0.0241 0.0004 0.0054 0.0001 0.0012 0.0008
Cell Periphery 0.0006 0.0003 0.0002 0.0002 0.0001 0.0001 0.0007 0.0002 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0 0.0003 0.0001
Cytoplasm 0.6965 0.863 0.7565 0.7646 0.7748 0.838 0.5487 0.8353 0.6742 0.7055 0.8973 0.7979 0.5795 0.8532 0.7747 0.85 0.6562 0.8934
Cytoplasmic Foci 0.0175 0.0075 0.0145 0.0138 0.0384 0.0146 0.0265 0.0169 0.0166 0.0493 0.0139 0.0147 0.0218 0.0048 0.0159 0.0158 0.0204 0.0083
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0003 0 0.0004 0.0001 0.0001 0.0001 0.0004 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0054 0.0053 0.0025 0.0077 0.0018 0.002 0.0064 0.0018 0.0043 0.0015 0.003 0.003 0.0361 0.0012 0.0077 0.001 0.0126 0.0029
Endosome 0.0149 0.0042 0.0092 0.014 0.0197 0.0076 0.0353 0.0055 0.0188 0.0148 0.0088 0.0121 0.0499 0.0026 0.0205 0.0237 0.0542 0.0203
Golgi 0.0035 0.0011 0.0021 0.0014 0.0072 0.002 0.009 0.0004 0.0075 0.0055 0.0064 0.0055 0.0107 0.0002 0.0044 0.0005 0.009 0.0108
Lipid Particles 0.0082 0.0002 0.0032 0.0004 0.0135 0.003 0.0107 0.0002 0.0098 0.0014 0.0008 0.0131 0.0067 0 0.0019 0 0.0088 0.0003
Mitochondria 0.0046 0.0005 0.0008 0.0012 0.0017 0.0013 0.0057 0.0002 0.0085 0.0671 0.0245 0.0024 0.0066 0.0001 0.002 0.0002 0.0038 0.0022
None 0.1996 0.1047 0.1795 0.1848 0.0935 0.1188 0.2623 0.1332 0.2033 0.1161 0.0168 0.1374 0.1162 0.1351 0.1305 0.1008 0.0861 0.0532
Nuclear Periphery 0.0038 0.0004 0.001 0.0014 0.0013 0.0006 0.0028 0.0004 0.0006 0.0004 0.0007 0.0003 0.0219 0.0002 0.0031 0.0004 0.0426 0.0006
Nucleolus 0.0004 0.0001 0.0001 0.0001 0.0003 0.0001 0.0017 0.0001 0.0001 0.0001 0.0001 0.0001 0.0013 0 0.0001 0 0.001 0.0001
Nucleus 0.0034 0.0007 0.0009 0.0014 0.0008 0.0008 0.0016 0.0006 0.0007 0.0007 0.0011 0.0005 0.0236 0.0004 0.0016 0.0007 0.0098 0.0007
Peroxisomes 0.0069 0.0002 0.0058 0.0002 0.0114 0.0014 0.0068 0.0001 0.0149 0.0097 0.0149 0.0036 0.0036 0 0.0039 0 0.0049 0.0001
Punctate Nuclear 0.0049 0.0004 0.0015 0.0011 0.0074 0.0017 0.0032 0.0004 0.0009 0.001 0.0004 0.0003 0.0046 0.0001 0.0009 0.0001 0.0018 0.0001
Vacuole 0.0031 0.0016 0.0021 0.003 0.0014 0.001 0.0127 0.0021 0.003 0.0034 0.0012 0.002 0.0087 0.0011 0.0044 0.0049 0.0131 0.0027
Vacuole Periphery 0.0006 0.0002 0.0004 0.0006 0.0004 0.0002 0.0028 0.0002 0.0006 0.0006 0.0004 0.0004 0.0026 0.0001 0.0013 0.0011 0.0075 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.9616 11.8402 14.1926 13.5928 13.2097 15.2741 12.2018 11.8092 12.1664 15.5458
Translational Efficiency 0.8847 1.1607 0.9377 1.0867 1.1137 0.9064 1.1462 1.1437 0.9364 0.9629

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 586 201 1265 1432 1809 173 1018 1521 2395 374 2283

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.74 846.48 1012.83 948.61 758.00 838.98 1061.98 970.12 755.47 840.82 1035.57 958.20
Standard Deviation 101.92 105.48 120.97 155.70 98.68 103.08 136.34 146.74 99.39 103.72 130.62 152.15
Intensity Change Log 2 — 0.244057 0.502902 0.408397 — 0.146439 0.486487 0.355965 — 0.194640 0.494476 0.381649

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000722 0.000287 0.000463 0.000799 0.000174 0.000252 0.000416 0.001177 0.000206 0.000261 0.000441 0.000968
Bud Neck 0.048219 0.010724 0.001221 0.001042 0.006735 0.009040 0.001940 0.002015 0.009163 0.009452 0.001554 0.001476
Bud Site 0.082009 0.038408 0.019454 0.011400 0.009895 0.019962 0.025321 0.029709 0.014115 0.024475 0.022168 0.019564
Cell Periphery 0.000620 0.000120 0.000173 0.000070 0.000146 0.000095 0.000144 0.000076 0.000174 0.000101 0.000160 0.000073
Cytoplasm 0.344013* 0.551935* 0.666990* 0.738841* 0.557849* 0.622351* 0.570007* 0.721020* 0.545336* 0.605122* 0.622129* 0.730895*
Cytoplasmic Foci 0.237527* 0.247807* 0.049588 0.041149 0.227095* 0.161898 0.080784 0.049620 0.227705* 0.182918* 0.064018 0.044926
Eisosomes 0.000297 0.000051 0.000045 0.000017 0.000080 0.000049 0.000051 0.000021 0.000093 0.000049 0.000048 0.000019
Endoplasmic Reticulum 0.002508 0.000704 0.001454 0.000303 0.004929 0.000989 0.000946 0.000256 0.004787 0.000919 0.001219 0.000282
Endosome 0.043876 0.029478 0.010739 0.003510 0.024245 0.020645 0.011137 0.005688 0.025393 0.022806 0.010923 0.004481
Golgi 0.007172 0.009292 0.000337 0.000737 0.005778 0.004392 0.000367 0.003195 0.005859 0.005591 0.000351 0.001833
Lipid Particles 0.006305 0.001746 0.002704 0.000565 0.004872 0.001720 0.001335 0.000657 0.004956 0.001726 0.002071 0.000606
Mitochondria 0.027628 0.003781 0.000073 0.000060 0.002325 0.002502 0.000116 0.001060 0.003805 0.002815 0.000093 0.000506
Mitotic Spindle 0.004154 0.003290 0.001459 0.002430 0.001514 0.005714 0.000932 0.015965 0.001669 0.005121 0.001215 0.008466
None 0.001471 0.000396 0.001209 0.001491 0.002013 0.001677 0.000669 0.003150 0.001981 0.001364 0.000959 0.002230
Nuclear Periphery 0.000300 0.000062 0.000460 0.000103 0.000285 0.000101 0.000222 0.000127 0.000286 0.000092 0.000350 0.000114
Nuclear Periphery Foci 0.002630 0.000512 0.009169 0.002097 0.000620 0.000344 0.009871 0.001747 0.000737 0.000385 0.009494 0.001941
Nucleolus 0.006269 0.000223 0.000163 0.000117 0.000502 0.000398 0.000116 0.000099 0.000840 0.000355 0.000141 0.000109
Nucleus 0.054796 0.011857 0.053559 0.030925 0.029582 0.019282 0.021288 0.023848 0.031057 0.017465 0.038631 0.027769
Peroxisomes 0.007824 0.005095 0.000394 0.000541 0.001527 0.002572 0.000709 0.001796 0.001895 0.003190 0.000540 0.001100
Vacuole 0.107344 0.080583 0.179989 0.163522 0.116217 0.123621 0.273319* 0.137794 0.115697 0.113091 0.223160* 0.152050
Vacuole Periphery 0.014317 0.003649 0.000357 0.000281 0.003619 0.002395 0.000308 0.000980 0.004245 0.002702 0.000334 0.000593

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.24 1.44 0.48 -0.82 -0.37 -1.47 -3.09 -1.31 -1.14 -0.88 -1.18 -3.59 -1.63 -1.44 -0.91
Bud Neck 3.30 4.13 4.17 7.29 2.59 -2.24 6.55 6.56 10.66 -0.04 -0.20 7.59 7.82 13.37 1.61
Bud Site 2.48 3.68 4.12 8.80 1.21 -6.00 -2.16 -3.75 0.84 0.31 -5.40 -0.91 0.42 6.47 1.16
Cell Periphery 2.00 1.83 2.24 4.33 5.83 1.89 0.85 4.21 1.45 5.34 2.65 1.08 4.78 2.42 7.70
Cytoplasm -6.74 -7.63 -12.09 -10.92 -5.00 -6.70 0.30 -6.53 -0.91 -3.41 -6.63 -2.15 -13.98 -8.22 -5.99
Cytoplasmic Foci -0.39 8.70 9.31 24.27 2.77 10.44 21.40 35.05 26.66 4.39 7.54 29.40 38.18 36.17 5.14
Eisosomes 2.29 2.32 2.61 7.17 6.52 3.52 4.60 9.01 3.97 5.58 4.36 4.99 8.38 5.89 8.60
Endoplasmic Reticulum 2.80 1.49 3.46 3.53 7.17 4.09 4.33 5.02 2.16 3.98 4.33 4.06 5.18 2.60 8.06
Endosome 1.94 4.72 5.67 10.56 5.28 2.56 10.70 16.81 14.38 4.77 1.91 12.17 19.83 19.22 7.16
Golgi -1.17 5.29 5.10 8.04 -0.89 2.37 12.61 5.40 3.72 -1.25 0.60 13.39 9.41 8.76 -1.47
Lipid Particles 2.72 2.33 3.44 4.62 4.01 8.37 9.28 11.66 5.83 2.77 8.90 7.45 12.54 7.93 4.72
Mitochondria 1.89 2.19 2.20 2.52 0.82 -0.16 4.39 3.33 2.75 -1.43 0.94 4.22 4.01 3.95 -1.39
Mitotic Spindle 0.35 1.34 0.80 0.44 -1.32 -2.83 1.01 -3.79 -1.90 -4.32 -2.70 0.76 -3.38 -0.68 -4.50
None 1.54 0.68 0.37 -2.48 -0.60 0.42 2.06 -0.03 -0.45 -2.01 0.84 1.86 0.59 -0.39 -2.05
Nuclear Periphery 1.69 -1.79 1.04 -6.74 8.77 3.84 -0.27 -0.47 -8.39 -0.23 4.29 -3.00 1.38 -9.00 7.03
Nuclear Periphery Foci 1.70 -4.26 0.46 -8.90 8.10 3.60 -8.71 -5.08 -6.20 7.23 3.36 -12.87 -7.53 -11.15 10.83
Nucleolus 1.89 1.91 1.92 4.30 1.26 0.90 2.62 2.59 4.06 -0.52 2.11 3.10 3.15 4.50 0.92
Nucleus 2.92 -0.22 1.31 -10.48 3.85 6.51 1.29 -3.16 -7.72 -3.44 8.11 -3.32 -2.58 -11.54 1.83
Peroxisomes 1.37 3.85 3.82 8.15 -0.42 -4.05 7.17 2.02 4.37 -1.18 -4.66 9.09 6.11 9.66 -1.23
Vacuole 1.45 -5.57 -4.75 -14.79 2.50 -1.62 -9.39 -14.53 -13.73 2.95 0.21 -11.83 -16.34 -17.90 3.89
Vacuole Periphery 3.03 4.02 4.04 6.23 1.62 4.07 14.00 10.68 7.32 -0.88 4.30 12.83 12.34 10.99 -0.24
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Assembly factor for the eIF2 translation initiation factor complex; regulates translational initiation; conserved residues of this ATP-Grasp protein that bind to ATP-Mg2+ in the pombe ortholog are required for complex assembly in budding yeast; interaction with eIF2 subunit Gcd11p facilitates complex assembly and activity; required for the START transition and timely progression through G2; regulated by nutrient availability; human ortholog complements the yeast mutant
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cdc123

Cdc123


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc123-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available