Standard name
Human Ortholog
Description Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 – 0 0 0 0 0.06 0.09 0.13 0.08 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0.09 – 0.06 0.08 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.05 0 0 0 0.13 0 – 0.11 0 0.1 0.09 0 0 0 0.08 0.09 0.09 0 0 0 0 0 0
Nucleus 0.24 0.24 0.15 0.08 0.07 0.05 0.06 – 0.08 0.06 0.05 0.08 0.08 0 0.07 0.17 0.16 0.24 0.13 0.18 0.1 0.05 0.07 0.07
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 0.83 0.86 0.9 0.87 0.86 0.82 – 0.83 0.8 0.83 0.78 0.9 0.86 0.81 0.78 0.78 0.77 0.77 0.73 0.78 0.78 0.72 0.69
Peroxisomes 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.12 0.29 0.37 0.38 0.48 – 0.33 0.45 0.39 0.43 0.12 0.14 0.13 0 0 0 0 0 0.07 0.1 0.12 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 4 0 – 4 1 3 9 0 1 0 0 0 0 3 4 2 0 3 7
Bud Neck 0 0 7 4 8 3 2 – 4 6 6 5 5 8 2 0 1 0 2 0 0 1 3 3
Bud Site 2 2 0 0 0 10 3 – 22 15 16 23 1 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 8 11 3 5 11 – 11 14 4 4 19 39 47 21 14 11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 2 1 0 0 0 0 0 0
Endosome 3 5 4 7 26 12 43 – 33 50 25 30 5 9 8 2 3 1 4 2 3 1 5 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 3 0 0 1 1 1
Mitochondria 31 20 0 4 7 47 11 – 65 26 42 52 3 7 1 20 17 18 2 1 0 1 2 3
Nucleus 68 93 48 18 29 18 29 – 47 38 20 45 26 16 25 42 30 49 38 74 34 11 28 26
Nuclear Periphery 1 4 0 0 1 0 3 – 3 1 1 2 0 0 1 0 0 0 1 2 0 1 1 4
Nucleolus 224 317 286 214 344 301 413 – 475 494 342 448 281 390 288 197 150 158 230 302 275 163 288 257
Peroxisomes 6 0 3 1 8 5 22 – 14 30 11 24 7 16 8 0 0 1 0 0 0 0 0 0
SpindlePole 1 8 39 69 144 132 243 – 190 279 161 245 36 65 48 7 4 0 6 11 25 21 50 41
Vac/Vac Membrane 0 4 3 0 2 3 4 – 2 0 1 1 1 2 2 6 8 3 5 10 5 1 10 14
Unique Cell Count 279 381 331 237 394 350 503 570 620 411 572 313 453 357 254 192 206 299 416 353 209 403 375
Labelled Cell Count 339 456 398 328 572 540 784 870 954 632 888 384 553 431 298 229 242 299 416 353 209 403 375


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.7 14.2 14.4 12.4 11.6 7.8 9.5 – 7.9 8.6 7.9 8.1 14.4 14.0 13.6 20.5 20.2 18.3 13.6 14.3 15.1
Std Deviation (1e-4) 7.2 4.1 3.9 3.3 3.0 2.3 2.4 – 2.4 2.2 2.1 2.3 5.2 5.4 5.5 10.4 8.6 8.5 3.6 4.5 4.1
Intensity Change (Log2) – – – -0.22 -0.32 -0.89 -0.6 – -0.86 -0.75 -0.87 -0.84 0.0 -0.04 -0.08 0.51 0.49 0.35 -0.08 -0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 -0.1 0 0 – 0 0 -0.7 0 -0.5 -0.4 0 0 0 0
Bud Site 0 0 0 0 – 3.6 2.9 3.6 3.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 -1.8 -0.9 -0.2 – -0.5 -0.2 -1.5 0 2.3 3.6 5.2 3.2 2.7 1.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.6 1.9 4.5 – 3.3 4.4 3.4 3.1 0 0.8 1.0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.9 0 – 6.4 3.8 6.0 5.7 0 0 0 5.2 5.5 5.5
Nucleus -2.5 -3.1 -4.1 -4.3 – -2.9 -4.3 -4.5 -3.2 -2.5 -5.5 -3.2 0.7 0.3 2.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.4 0.4 -0.2 -1.6 – -1.2 -2.6 -1.2 -3.0 1.3 -0.1 -2.0 -2.8 -2.5 -2.9
Peroxisomes 0 1.2 0 2.9 – 1.6 3.2 1.8 2.8 0 2.4 1.4 0 0 0
SpindlePole 5.2 7.6 7.8 10.9 – 7.2 10.3 8.4 9.7 -0.1 1.0 0.7 -4.0 -3.9 -5.1
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1482 8.7125 7.5427 7.2778 6.8473 7.1678 11.3248 12.5994 11.4885 9.0148 10.1481 10.5933 13.5806 15.6366 13.2434 12.9909 12.527 13.6703
Actin 0.0236 0 0.0152 0.0002 0.0316 0 0.0122 0 0.0055 0.0021 0.0283 0.0015 0.0038 0 0 0 0.0005 0
Bud 0.0004 0 0.0003 0.0001 0.0002 0 0.0001 0 0 0.0021 0.0004 0 0 0 0 0 0 0
Bud Neck 0.0029 0.0001 0.0006 0.0002 0.001 0.0001 0.0006 0.0001 0.0001 0.0002 0.0013 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001
Bud Periphery 0.0007 0 0.0004 0 0.0004 0 0.0002 0 0 0.0033 0.0007 0.0001 0 0 0 0 0.0001 0
Bud Site 0.0026 0 0.0013 0.0005 0.0007 0.0001 0.0008 0 0.0002 0.0033 0.0018 0.0001 0.0002 0 0 0 0.0002 0
Cell Periphery 0.0003 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0025 0 0.0004 0.0011 0.0007 0.0006 0.0011 0 0.0008 0.0003 0.0009 0.0001 0 0 0 0 0.0032 0
Cytoplasmic Foci 0.0177 0 0.0088 0.0263 0.0111 0.0025 0.0085 0 0.0003 0.0033 0.0018 0.0004 0.0004 0 0.0001 0.0001 0.0005 0.0001
Eisosomes 0.0004 0 0.0001 0.0001 0.0006 0 0.0001 0 0 0 0.0005 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0 0.0002 0.0003 0.0012 0.0001 0.0004 0 0.0002 0.0002 0.0022 0.0007 0.0001 0 0 0 0.0009 0
Endosome 0.0158 0 0.0032 0.001 0.0044 0.0004 0.0048 0 0.0006 0.006 0.0133 0.0022 0.0004 0 0 0 0.0027 0
Golgi 0.004 0 0.0027 0.0005 0.0041 0.0001 0.0023 0 0.0004 0.0025 0.0036 0.0011 0.0003 0 0 0 0.0001 0
Lipid Particles 0.0195 0.0001 0.004 0.0239 0.0301 0.0014 0.0066 0 0.0009 0.0025 0.0063 0.0023 0.001 0 0.0002 0.0002 0.0005 0.0004
Mitochondria 0.0052 0.0001 0.005 0.0037 0.0179 0.0002 0.0036 0 0.0002 0.0129 0.0038 0.0072 0.0004 0 0.0001 0 0.0002 0.0001
None 0.0032 0 0.0027 0.0023 0.0017 0.0003 0.0017 0 0.0003 0.0005 0.0032 0.0005 0.0001 0 0 0 0.0025 0
Nuclear Periphery 0.0062 0.0001 0.0008 0.0017 0.0032 0.0011 0.0077 0 0.0028 0.0004 0.0128 0.0038 0.0002 0 0.0001 0 0.0109 0.0001
Nucleolus 0.7905 0.8926 0.8466 0.8705 0.7908 0.8986 0.8564 0.8338 0.8939 0.8156 0.8038 0.8745 0.9048 0.8669 0.9266 0.8893 0.8593 0.905
Nucleus 0.0713 0.1045 0.0966 0.0559 0.0893 0.0859 0.0811 0.1657 0.0913 0.1374 0.1042 0.0993 0.0812 0.1325 0.0715 0.1041 0.1092 0.0887
Peroxisomes 0.0207 0 0.0036 0.0009 0.002 0.0001 0.0017 0 0.0001 0.004 0.0012 0.0005 0.0003 0 0 0 0.0001 0
Punctate Nuclear 0.0078 0.0023 0.0056 0.0078 0.0049 0.0081 0.0087 0.0002 0.0019 0.0008 0.0075 0.0049 0.0063 0.0002 0.0006 0.0059 0.0081 0.0052
Vacuole 0.0024 0.0001 0.001 0.001 0.001 0.0003 0.0006 0.0001 0.0002 0.0017 0.0009 0.0002 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001
Vacuole Periphery 0.0015 0.0001 0.0007 0.0019 0.0027 0.0002 0.0007 0 0.0001 0.0008 0.0014 0.0003 0.0001 0 0.0003 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.2708 44.6324 32.0152 30.8757 53.9446 46.5735 38.3586 25.3818 31.7979 43.8863
Translational Efficiency 3.0146 2.5793 2.4523 2.3657 1.8093 2.0992 1.6525 2.366 1.6937 1.9466

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
Localization
Cell Percentages nucleolus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rsa3

Rsa3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsa3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available