ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.98 0.98 1.0 0.97 0.98 0.95 0.86 0.84 0.73 0.73 0.7 0.66 0.98 1.0 0.99 0.98 0.98 0.94 0.96 0.89 0.97 0.91 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.15 0.16 0.29 0.27 0.36 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.09 0.09 0.08 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud 1 0 1 0 1 0 3 0 1 1 2 0 0 0 0 0 0 0 0 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 0 1 1 4 1 9 7 5 3 3 2 1 5 2 9 0 0 0 0 0 0
Cytoplasm 235 131 175 116 176 173 194 244 160 228 116 128 316 344 321 245 204 152 228 120 167 155 225 264
Endoplasmic Reticulum 2 1 0 2 0 1 9 5 2 9 5 6 1 0 4 8 6 4 1 0 0 0 1 1
Endosome 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 1 1 0 2 1 0 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0
Mitochondria 3 1 0 5 2 15 34 46 63 85 59 74 0 0 0 2 2 3 0 0 0 0 1 2
Nucleus 0 1 0 0 2 0 2 7 3 5 4 3 4 4 1 4 0 1 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 3 2 17 19 29 14 14 1 1 0 7 7 8 0 4 0 7 7 3
Unique Cell Count 239 133 175 119 180 183 225 290 219 312 166 195 321 344 325 250 209 161 238 135 172 172 244 280
Labelled Cell Count 241 135 178 123 183 193 249 320 257 366 206 229 326 351 327 272 223 177 238 135 172 172 244 280


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 8.9 7.5 7.0 8.0 7.6 7.1 7.2 6.9 6.7 6.2 6.3 9.2 9.6 9.2 12.2 11.6 11.5 8.5 9.2 10.4
Std Deviation (1e-4) 0.9 1.7 1.3 1.2 1.3 1.5 1.5 1.8 1.8 1.4 1.4 1.3 1.5 1.6 1.3 2.9 3.5 2.6 1.8 1.7 1.7
Intensity Change (Log2) – – – -0.11 0.1 0.01 -0.09 -0.07 -0.12 -0.17 -0.28 -0.25 0.3 0.36 0.29 0.7 0.63 0.62 0.19 0.3 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3
Cytoplasm -2.1 -2.0 -3.1 -5.1 -5.6 -7.4 -7.5 -7.9 -8.6 -1.7 0 -1.5 -1.9 -2.1 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.9 5.4 5.6 7.7 7.6 8.7 9.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 4.0 4.2 3.9 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.5636 10.2341 8.7886 9.0535 6.6361 9.0208 6.5806 10.1042 8.7135 8.9498 9.079 9.2254 7.337 9.8956 8.5847 7.321 8.3016 8.7152
Actin 0.026 0.0014 0.0155 0.0367 0.0809 0.0091 0.0362 0.0017 0.0022 0.0271 0 0.0042 0.0103 0.0001 0.0061 0.0014 0.0584 0.0061
Bud 0.0004 0.0001 0.0006 0.0004 0.0013 0.0001 0.0041 0.0001 0.0005 0.0008 0 0.0002 0.0001 0.0001 0.003 0.0001 0.0011 0.0001
Bud Neck 0.0011 0.0002 0.0007 0.0023 0.0029 0.0009 0.0038 0.0001 0.0046 0.0184 0.0003 0.001 0.0002 0.0001 0.0009 0.0005 0.001 0.001
Bud Periphery 0.0004 0 0.0005 0.0005 0.0013 0 0.0035 0.0001 0.0004 0.0008 0 0.0001 0 0 0.0023 0 0.0014 0.0001
Bud Site 0.0021 0.0004 0.0011 0.0037 0.0085 0.0001 0.0082 0.0002 0.0066 0.0075 0 0.0001 0.0001 0.0002 0.0009 0.0001 0.0008 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0005 0 0.0002 0.0003 0 0 0 0 0 0 0.0001 0
Cytoplasm 0.8753 0.9881 0.9394 0.9172 0.754 0.9582 0.6838 0.9892 0.9355 0.8146 0.9872 0.9652 0.9649 0.9956 0.9525 0.9861 0.831 0.963
Cytoplasmic Foci 0.0132 0.0016 0.0058 0.0043 0.0164 0.0042 0.0525 0.0007 0.0073 0.0081 0.0012 0.0058 0.0028 0.0006 0.0047 0.0017 0.009 0.0028
Eisosomes 0.0003 0 0.0001 0.0008 0.0003 0 0.0004 0 0 0.0002 0 0 0 0 0.0001 0 0.0008 0
Endoplasmic Reticulum 0.0036 0.001 0.0016 0.0031 0.0087 0.0016 0.0068 0.0021 0.0016 0.001 0.0004 0.0011 0.0014 0.0002 0.0006 0.0005 0.0046 0.0015
Endosome 0.012 0.0004 0.0046 0.0022 0.0425 0.0043 0.0757 0.001 0.0199 0.0459 0.0004 0.0076 0.0024 0.0001 0.0015 0.0004 0.0437 0.0046
Golgi 0.0054 0.0001 0.0038 0.0015 0.0103 0.0006 0.0218 0.0002 0.0082 0.0136 0 0.0063 0.0002 0 0.0014 0 0.0181 0.0038
Lipid Particles 0.0111 0 0.0033 0.0029 0.0271 0.0006 0.0113 0.0001 0.0009 0.0037 0 0.0002 0.0001 0 0.0045 0 0.0067 0.0023
Mitochondria 0.0029 0.0001 0.0067 0.0017 0.0064 0.0003 0.0409 0.0002 0.0018 0.0258 0 0.0014 0.0001 0 0.0013 0.0001 0.006 0.004
None 0.0171 0.0034 0.003 0.0021 0.0073 0.0015 0.0121 0.0011 0.0015 0.0025 0.0015 0.0008 0.0103 0.0012 0.0024 0.0025 0.0015 0.0012
Nuclear Periphery 0.0063 0.0005 0.0009 0.0022 0.0064 0.007 0.0048 0.0007 0.0009 0.0009 0.0008 0.0006 0.001 0.0002 0.0008 0.0005 0.0053 0.0032
Nucleolus 0.0005 0 0.0001 0.0004 0.0004 0.0001 0.0008 0 0.0004 0.0005 0 0 0 0 0.0003 0 0.0001 0.0001
Nucleus 0.0037 0.0022 0.0033 0.0057 0.0034 0.007 0.0053 0.002 0.0032 0.005 0.0071 0.0044 0.0044 0.0015 0.0083 0.0056 0.0045 0.0044
Peroxisomes 0.0138 0 0.0075 0.0092 0.0112 0.001 0.0067 0 0.0007 0.0013 0 0.0004 0.0001 0 0.0068 0 0.0014 0.0007
Punctate Nuclear 0.0037 0.0002 0.0008 0.0022 0.0017 0.0026 0.0095 0.0001 0.0005 0.0006 0.0007 0.0002 0.0012 0.0001 0.0011 0.0003 0.0016 0.0006
Vacuole 0.0007 0.0002 0.0004 0.0005 0.0067 0.0006 0.0093 0.0002 0.0024 0.0156 0.0002 0.0003 0.0003 0 0.0003 0.0002 0.0016 0.0004
Vacuole Periphery 0.0004 0 0.0001 0.0001 0.0019 0.0003 0.002 0 0.0008 0.0059 0 0.0002 0.0001 0 0.0001 0 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1846 32.2345 39.467 31.7479 24.5448 33.3361 35.0367 39.8706 34.1606 31.1045
Translational Efficiency 1.9808 2.0187 1.0975 1.2735 1.5315 1.5265 1.8213 1.1447 1.343 1.4747

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
88 120 753 1164 143 1631 275 1077 231 1751 1028 2241

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 908.66 1113.20 1205.68 1329.96 883.44 968.13 1313.11 1318.49 893.05 978.07 1234.42 1324.45
Standard Deviation 104.28 148.45 176.25 221.62 114.70 131.39 179.99 211.76 111.52 137.60 183.53 217.01
Intensity Change Log 2 — 0.292900 0.408035 0.549570 — 0.132069 0.571784 0.577683 — 0.215855 0.491080 0.563497

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000246 0.000534 0.000274 0.006427 0.000629 0.000414 0.000995 0.000649 0.000483 0.000422 0.000467 0.003650
Bud Neck 0.002720 0.001757 0.002104 0.005074 0.001556 0.002090 0.019461 0.004002 0.002000 0.002067 0.006747 0.004559
Bud Site 0.015628 0.017184 0.011204 0.056181 0.011688 0.010754 0.021479 0.032204 0.013189 0.011195 0.013953 0.044658
Cell Periphery 0.000179 0.000159 0.000178 0.000201 0.000385 0.000054 0.000178 0.000118 0.000307 0.000061 0.000178 0.000161
Cytoplasm 0.581096* 0.649995* 0.598859* 0.581322* 0.707306* 0.769967* 0.540676* 0.575105* 0.659226* 0.761745* 0.583295* 0.578334*
Cytoplasmic Foci 0.198242* 0.128443 0.021071 0.021242 0.143073 0.075168 0.015431 0.033637 0.164090 0.078819 0.019562 0.027199
Eisosomes 0.000081 0.000045 0.000040 0.000019 0.000058 0.000018 0.000037 0.000016 0.000067 0.000020 0.000039 0.000018
Endoplasmic Reticulum 0.001320 0.001667 0.001746 0.002575 0.002531 0.000441 0.002403 0.000728 0.002070 0.000525 0.001922 0.001688
Endosome 0.012062 0.011997 0.004809 0.010697 0.007674 0.005226 0.005807 0.026031 0.009346 0.005690 0.005076 0.018066
Golgi 0.000865 0.001710 0.000161 0.005350 0.000679 0.001824 0.000389 0.019823 0.000750 0.001816 0.000222 0.012305
Lipid Particles 0.002862 0.001823 0.001400 0.003171 0.002869 0.000729 0.002272 0.000905 0.002866 0.000804 0.001633 0.002082
Mitochondria 0.000060 0.001561 0.000394 0.003933 0.000154 0.002566 0.000291 0.007647 0.000118 0.002497 0.000366 0.005718
Mitotic Spindle 0.000245 0.018994 0.010706 0.059004 0.000480 0.010915 0.003774 0.088031 0.000391 0.011468 0.008852 0.072954
None 0.000721 0.001446 0.008249 0.003820 0.002437 0.002071 0.007003 0.003361 0.001783 0.002028 0.007916 0.003599
Nuclear Periphery 0.000262 0.000181 0.000745 0.000791 0.000162 0.000202 0.000788 0.002110 0.000200 0.000201 0.000757 0.001425
Nuclear Periphery Foci 0.003197 0.007906 0.003727 0.006177 0.005937 0.001209 0.009953 0.006587 0.004893 0.001668 0.005393 0.006374
Nucleolus 0.000212 0.000273 0.000477 0.000206 0.000248 0.000294 0.000373 0.000276 0.000234 0.000292 0.000449 0.000240
Nucleus 0.042730 0.032586 0.172647* 0.070243 0.064748 0.042074 0.113468 0.035563 0.056360 0.041424 0.156816* 0.053576
Peroxisomes 0.001439 0.002391 0.000362 0.003947 0.000734 0.001559 0.000960 0.002658 0.001003 0.001616 0.000522 0.003327
Vacuole 0.135522 0.118355 0.160509 0.154675 0.046218 0.071242 0.253804* 0.155163 0.080238 0.074471 0.185466 0.154910
Vacuole Periphery 0.000312 0.000991 0.000335 0.004944 0.000434 0.001184 0.000458 0.005385 0.000387 0.001170 0.000368 0.005156

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.78 -2.48 -1.90 -1.19 -1.61 1.37 -0.86 2.69 0.81 1.87 0.64 -0.44 -0.92 -1.22 -0.68
Bud Neck 1.89 0.03 -0.19 -3.47 -0.41 -1.47 -2.03 -2.16 -0.54 1.69 -0.10 -2.51 -1.98 -2.09 1.70
Bud Site -0.43 1.18 0.20 1.04 -2.12 0.77 -0.39 0.26 -0.64 0.62 1.01 1.14 0.14 -1.37 -1.48
Cell Periphery 0.53 0.59 1.35 0.58 1.28 6.81 4.47 6.31 -4.29 5.02 7.66 4.39 5.98 -7.43 4.94
Cytoplasm -2.06 1.92 2.33 5.40 0.79 -2.88 13.04 8.60 20.58 -7.60 -5.93 9.63 8.06 27.67 -3.51
Cytoplasmic Foci 3.30 10.91 11.07 9.40 1.79 5.90 11.13 11.32 20.99 -0.07 8.58 15.44 15.64 23.86 1.07
Eisosomes 3.17 4.43 6.54 4.81 8.66 6.25 2.95 6.87 2.86 9.39 8.48 5.20 9.34 3.27 12.04
Endoplasmic Reticulum -0.43 -1.69 1.53 1.80 6.46 3.62 1.43 3.80 1.87 7.26 3.93 0.32 3.94 0.24 9.93
Endosome 0.51 3.92 4.67 3.40 3.50 2.42 4.46 2.68 0.87 -1.38 3.55 5.53 5.56 3.46 0.84
Golgi -0.58 1.90 0.44 0.80 -1.63 -2.05 3.96 -1.94 -0.85 -2.49 -1.95 3.72 -1.58 0.13 -2.91
Lipid Particles 1.00 1.12 0.36 -1.75 -3.95 3.71 1.65 3.00 -4.13 4.84 3.88 2.13 1.95 -7.93 -0.75
Mitochondria -1.15 -5.04 -2.80 0.65 -0.99 -2.65 -1.25 -1.53 1.29 -1.43 -2.79 -4.22 -2.30 1.79 -1.55
Mitotic Spindle -1.65 -4.59 -7.96 -0.57 -2.86 -5.31 -4.25 -7.13 -3.54 -4.51 -5.60 -5.50 -10.52 -3.98 -4.65
None -1.42 -5.48 -7.97 -5.70 1.71 0.80 -3.13 -1.27 -3.84 2.72 -0.43 -5.11 -4.64 -5.96 2.85
Nuclear Periphery 0.91 -10.05 -6.89 -13.33 4.99 -1.11 -12.97 -3.88 -3.49 2.09 -0.25 -18.22 -7.16 -6.79 3.84
Nuclear Periphery Foci -2.66 -1.14 -0.07 2.80 1.84 4.58 -0.25 2.85 -4.14 4.09 4.56 0.37 2.58 -5.09 3.97
Nucleolus -0.32 -1.93 0.48 0.53 2.42 -0.68 -0.22 1.61 2.00 2.05 -1.02 -1.66 1.63 2.08 2.79
Nucleus 0.89 -17.40 -7.61 -12.10 14.37 2.96 -8.24 0.41 -6.74 10.38 2.35 -18.46 -4.61 -14.82 18.88
Peroxisomes -0.58 3.74 2.14 1.42 -1.23 -2.11 -0.17 1.51 2.78 0.68 -1.41 3.26 2.36 2.83 -0.71
Vacuole 0.94 -3.41 -8.88 -12.80 -8.73 -4.06 -16.67 -23.21 -26.05 2.50 0.01 -13.86 -21.79 -35.36 -6.25
Vacuole Periphery -0.90 1.36 -0.98 0.32 -1.24 -1.47 4.65 -1.10 0.68 -2.32 -1.67 3.73 -1.35 0.86 -2.29
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR225C

YLR225C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr225c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available