Standard name
Human Ortholog
Description Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.14 0.16 0.07 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0 0 0.08 0.18 0.28 0.16 0.19 0.22 0.24 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.94 0.92 0.84 0.83 0.76 0.75 0.74 0.76 0.73 0.73 0.91 0.84 0.81 0.88 0.88 0.9 0.94 0.94 0.88 0.87 0.74 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.07 0 0.1 0.17 0.23 0.25 0.32 0.31 0.38 0.38 0.34 0 0 0 0 0 0 0 0 0 0 0.08 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 1 3 3 1 4 2 6 0 0 0 1 0 0 2 1 1 1 2 3
Bud Neck 0 0 0 0 1 0 0 1 4 3 3 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 1 2 1 0 5 6 17 21 16 25 29 26 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 2 13 8 17 14 15 5 6 10 6 5 23 65 84 26 38 11 1 0 1 3 3 4
Endoplasmic Reticulum 0 0 1 0 0 0 1 0 0 0 0 1 0 0 0 6 4 2 0 0 1 1 0 2
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 1 2 4 1 1 2 0 2 6 2 4 2
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 2 0 2 3
Mitochondria 22 15 8 10 27 88 178 113 94 148 146 153 0 0 1 5 4 4 0 0 3 1 4 8
Nucleus 279 307 379 247 274 397 488 529 368 498 449 470 264 399 430 341 450 276 281 319 371 350 275 364
Nuclear Periphery 0 0 0 2 1 8 8 4 8 6 3 4 0 1 2 4 1 1 0 0 1 0 0 2
Nucleolus 33 22 17 26 56 110 160 224 155 251 237 220 3 10 11 16 18 12 5 2 12 14 29 65
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 1 0 0 3 0 0 1 0 0 1 0 0 0
SpindlePole 1 0 0 1 1 0 1 0 2 0 0 3 1 8 13 2 2 2 1 1 1 1 2 2
Vac/Vac Membrane 1 2 1 0 1 0 9 4 1 1 1 3 1 4 4 9 9 7 4 8 15 20 44 75
Unique Cell Count 297 322 405 268 327 479 639 705 496 658 618 645 291 476 533 388 509 306 300 340 424 401 374 537
Labelled Cell Count 344 350 422 294 385 624 880 904 655 946 876 894 294 489 552 414 527 319 300 340 424 401 374 537


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 8.1 7.6 6.6 6.4 5.6 5.5 5.7 5.5 5.5 5.2 5.2 9.7 8.9 8.6 10.9 10.7 10.9 10.0 9.9 10.8
Std Deviation (1e-4) 1.7 2.0 2.0 1.6 1.8 1.8 1.5 1.3 1.3 1.6 1.2 1.3 2.7 2.3 2.8 3.0 2.8 2.7 1.8 1.7 2.2
Intensity Change (Log2) – – – -0.2 -0.23 -0.42 -0.46 -0.41 -0.46 -0.45 -0.55 -0.53 0.35 0.24 0.19 0.52 0.51 0.52 0.4 0.39 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.9 3.1 3.3 3.6 4.1 3.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 1.3 -0.2 -0.8 -3.2 -2.1 -1.8 -2.6 -3.0 2.8 5.4 6.3 2.3 2.8 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 4.0 7.8 10.6 7.2 8.0 9.2 9.5 9.5 0 0 0 -0.8 -1.6 -0.7
Nucleus -0.7 -4.2 -4.8 -7.2 -7.7 -7.7 -7.5 -8.3 -8.3 -1.4 -4.5 -5.7 -2.8 -2.7 -1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.9 5.8 7.9 8.7 10.7 10.3 12.4 12.4 11.3 -2.5 -1.8 -1.9 -0.1 -0.5 -0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.3497 5.8472 6.1571 5.3507 5.1294 5.7378 7.7225 7.8126 7.5192 7.6743 7.1854 7.3293 6.6933 5.9546 5.8229 5.7961 5.6561 5.7689
Actin 0.0001 0 0 0 0.0009 0 0.0069 0 0.0116 0 0.0098 0 0.0037 0 0.0003 0 0 0.0001
Bud 0 0 0 0 0.0002 0 0.0003 0 0.0001 0 0.0004 0 0 0 0 0 0 0
Bud Neck 0.0001 0.0001 0 0.0001 0.0005 0.0003 0.0006 0 0.0014 0 0.0007 0.0002 0.0001 0 0 0 0.0001 0.0002
Bud Periphery 0 0 0 0 0.0001 0 0.0008 0 0.0001 0 0.0007 0 0.0001 0 0 0 0 0
Bud Site 0.0001 0 0 0 0.0002 0 0.0013 0 0.0003 0 0.0022 0 0.0001 0 0.0002 0 0 0
Cell Periphery 0 0 0 0 0 0 0.0006 0 0.0001 0 0.0003 0 0 0 0 0 0 0
Cytoplasm 0.001 0 0 0 0.003 0 0.0021 0 0.002 0 0.0026 0.0001 0.0001 0 0.0001 0 0 0
Cytoplasmic Foci 0.0011 0 0 0 0.0113 0 0.0097 0 0.003 0 0.0015 0 0.0004 0 0.0004 0 0 0
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0003 0 0 0 0.0002 0 0.0019 0 0.0012 0 0.002 0 0.0001 0 0.0001 0 0 0
Endosome 0.0005 0 0 0 0.0044 0 0.008 0 0.0036 0 0.0083 0 0.0002 0 0.0001 0 0 0.0001
Golgi 0.0001 0 0 0 0.0003 0 0.0017 0 0.0006 0 0.0015 0 0.0002 0 0.0001 0 0 0.0001
Lipid Particles 0.0008 0 0 0 0.0015 0 0.0083 0 0.0012 0 0.002 0 0.0002 0 0.0006 0 0 0.0012
Mitochondria 0.0002 0.0001 0 0 0.0012 0 0.0087 0 0.0004 0 0.0091 0.0001 0.0002 0 0.0005 0.0001 0.0001 0.0033
None 0.0007 0 0 0 0.0102 0 0.0014 0 0.0008 0 0.0028 0 0.0004 0 0.0002 0 0 0
Nuclear Periphery 0.0016 0.0071 0.0003 0.0006 0.0128 0.0001 0.0121 0.0008 0.0041 0.0001 0.0286 0.0003 0.0006 0.0004 0.0013 0.0005 0.0002 0.0001
Nucleolus 0.0253 0.0196 0.007 0.0228 0.1733 0.0685 0.0256 0.0088 0.0092 0.0127 0.0821 0.0388 0.0147 0.0084 0.0084 0.0041 0.0831 0.039
Nucleus 0.9619 0.9725 0.9926 0.9759 0.7224 0.9304 0.8979 0.9902 0.9508 0.9871 0.8282 0.9602 0.9778 0.9909 0.9858 0.9951 0.916 0.955
Peroxisomes 0 0 0 0 0.0006 0 0.0032 0 0.0003 0 0.0005 0 0.0006 0 0.0001 0 0 0.0006
Punctate Nuclear 0.0058 0.0005 0.0001 0.0005 0.0557 0.0006 0.0049 0.0001 0.0032 0.0001 0.0109 0.0001 0.0003 0.0002 0.0015 0.0001 0.0004 0.0002
Vacuole 0.0002 0 0 0 0.0005 0 0.002 0 0.0046 0 0.0035 0.0001 0.0001 0 0.0001 0 0 0
Vacuole Periphery 0.0001 0.0001 0 0 0.0006 0 0.0019 0 0.0014 0 0.0023 0 0.0001 0 0.0002 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9319 6.6423 12.9382 16.355 6.0211 6.4979 14.0616 13.5985 15.6754 17.3815
Translational Efficiency 1.3718 3.0012 1.4994 1.2543 2.7225 2.3999 1.3105 1.2914 1.1228 0.9203

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1431 385 2170 716 1579 1599 115 977 3010 1984 2285 1693

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 887.40 1053.78 970.21 1091.51 837.92 957.02 1212.26 1109.99 861.44 975.80 982.39 1102.17
Standard Deviation 138.90 194.80 143.17 204.22 138.33 144.33 178.27 216.96 140.79 160.05 154.49 211.87
Intensity Change Log 2 — 0.247917 0.128712 0.298669 — 0.191737 0.532815 0.405662 — 0.220906 0.339098 0.351623

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000322 0.000446 0.000709 0.000725 0.000358 0.000606 0.001000 0.000891 0.000341 0.000575 0.000724 0.000821
Bud Neck 0.004955 0.006062 0.023511 0.015838 0.004899 0.009731 0.007934 0.014682 0.004926 0.009019 0.022727 0.015171
Bud Site 0.000548 0.000502 0.001758 0.002551 0.000523 0.001437 0.000901 0.002333 0.000535 0.001256 0.001714 0.002425
Cell Periphery 0.000067 0.000059 0.000111 0.000078 0.000093 0.000082 0.000039 0.000096 0.000080 0.000078 0.000107 0.000088
Cytoplasm 0.000527 0.000047 0.000609 0.000326 0.000805 0.000583 0.000127 0.000577 0.000673 0.000479 0.000585 0.000471
Cytoplasmic Foci 0.000167 0.000033 0.000146 0.000195 0.000021 0.001020 0.000020 0.002132 0.000091 0.000828 0.000139 0.001313
Eisosomes 0.000056 0.000047 0.000054 0.000034 0.000050 0.000063 0.000066 0.000038 0.000053 0.000060 0.000054 0.000036
Endoplasmic Reticulum 0.002773 0.001769 0.002537 0.002055 0.003514 0.003699 0.002564 0.002077 0.003162 0.003325 0.002538 0.002068
Endosome 0.000124 0.000044 0.000285 0.000784 0.000113 0.002080 0.000052 0.001256 0.000118 0.001685 0.000273 0.001056
Golgi 0.000130 0.000038 0.000136 0.000634 0.000042 0.000825 0.000071 0.002253 0.000084 0.000673 0.000132 0.001568
Lipid Particles 0.000023 0.000087 0.000081 0.000032 0.000024 0.000257 0.000041 0.000093 0.000023 0.000224 0.000079 0.000067
Mitochondria 0.000462 0.001043 0.001575 0.002073 0.000722 0.001783 0.001285 0.001681 0.000598 0.001640 0.001560 0.001847
Mitotic Spindle 0.000534 0.000802 0.001542 0.002265 0.000479 0.003087 0.000078 0.002597 0.000505 0.002644 0.001468 0.002456
None 0.001685 0.002005 0.001881 0.000977 0.001667 0.000960 0.001472 0.001289 0.001676 0.001162 0.001861 0.001157
Nuclear Periphery 0.000053 0.000061 0.000379 0.000766 0.000085 0.000308 0.000105 0.000379 0.000070 0.000260 0.000365 0.000543
Nuclear Periphery Foci 0.000149 0.000119 0.000098 0.000479 0.000193 0.000706 0.000034 0.001169 0.000172 0.000592 0.000095 0.000878
Nucleolus 0.025418 0.029273 0.016816 0.008796 0.025714 0.033058 0.017857 0.007291 0.025573 0.032324 0.016869 0.007927
Nucleus 0.961264* 0.956532* 0.946728* 0.957924* 0.959761* 0.934574* 0.964442* 0.956690* 0.960476* 0.938835* 0.947619* 0.957212*
Peroxisomes 0.000395 0.000758 0.000343 0.000262 0.000304 0.000701 0.001081 0.000407 0.000347 0.000712 0.000380 0.000346
Vacuole 0.000216 0.000153 0.000462 0.001762 0.000406 0.002044 0.000734 0.001192 0.000315 0.001677 0.000475 0.001433
Vacuole Periphery 0.000131 0.000119 0.000242 0.001444 0.000229 0.002394 0.000098 0.000876 0.000182 0.001952 0.000234 0.001116

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.90 -10.60 -9.04 -6.30 -0.91 -4.15 -7.80 -2.56 -1.44 0.77 -4.94 -11.19 -4.09 -2.24 -0.82
Bud Neck -1.43 -14.09 -6.98 -6.03 2.97 -5.43 -3.40 -8.82 -4.30 -4.56 -5.74 -14.37 -11.14 -6.26 4.18
Bud Site 0.23 -4.63 -1.93 -1.99 -0.85 -3.61 -2.32 -2.26 -1.12 -1.64 -3.45 -5.63 -2.95 -1.88 -1.08
Cell Periphery 0.26 -2.09 -0.58 -0.64 1.54 0.40 2.26 -0.03 -0.39 -1.96 0.13 -1.32 -0.34 -0.47 0.86
Cytoplasm 1.18 -0.23 -0.28 -1.78 -0.15 0.38 1.18 -0.93 -1.37 -1.97 0.49 0.22 -1.01 -1.53 -1.38
Cytoplasmic Foci 1.24 0.26 -0.06 -1.49 -0.34 -2.85 -0.37 -2.01 -0.84 -2.01 -2.57 -0.28 -1.99 -0.51 -1.90
Eisosomes 2.04 0.54 5.88 3.44 7.42 -2.78 -3.77 2.76 6.94 6.49 -2.13 -0.82 5.79 8.03 9.16
Endoplasmic Reticulum 5.63 0.98 3.41 -1.77 2.83 -0.73 0.88 7.19 6.17 1.81 -0.82 3.77 7.62 5.91 3.78
Endosome 1.58 -1.15 -1.54 -1.79 -1.11 -3.40 1.24 -2.19 1.33 -2.29 -3.35 -1.28 -2.66 1.36 -2.08
Golgi 1.23 -0.02 -1.17 -1.47 -1.17 -2.29 -2.97 -1.96 -1.10 -1.91 -2.11 -0.63 -2.19 -1.08 -2.10
Lipid Particles -0.77 -1.18 -1.02 0.54 0.78 -2.49 -1.97 -1.77 1.66 -0.61 -2.59 -1.29 -1.99 1.92 0.15
Mitochondria -1.18 -7.87 -8.86 -2.29 -2.44 -2.50 -2.50 -3.80 -0.12 -2.42 -3.23 -5.62 -7.85 -1.15 -1.98
Mitotic Spindle -0.23 -1.84 -1.57 -1.20 -0.46 -2.43 1.26 -1.63 0.42 -2.02 -2.40 -2.19 -2.22 0.22 -0.73
None -0.24 -0.33 1.19 0.85 3.32 2.23 0.73 0.95 -1.81 -0.12 1.33 -0.50 1.46 -0.20 2.68
Nuclear Periphery -0.12 -4.90 -2.60 -2.56 -1.26 -2.75 -1.88 -4.23 -0.80 -2.92 -2.90 -4.73 -3.94 -2.07 -1.13
Nuclear Periphery Foci 0.85 0.93 -0.11 -1.31 -1.48 -1.64 1.28 -1.04 -0.39 -1.21 -1.08 1.16 -0.99 -0.36 -1.41
Nucleolus -1.09 3.91 8.65 5.93 5.28 -3.50 1.96 9.86 13.15 2.38 -3.91 4.74 13.21 14.52 7.03
Nucleus 0.93 5.73 1.50 0.54 -2.06 6.23 -0.21 1.58 -3.37 1.34 6.51 6.16 2.19 -3.36 -2.41
Peroxisomes -2.14 0.46 1.52 3.06 0.84 -4.66 -3.80 -0.80 1.54 2.52 -4.50 -0.50 -0.21 2.94 0.17
Vacuole 1.18 -3.66 -2.43 -2.52 -2.10 -3.46 -1.32 -2.57 0.70 0.57 -3.64 -2.28 -3.52 -0.34 -2.87
Vacuole Periphery 0.45 -1.76 -1.83 -1.85 -1.66 -2.87 1.38 -1.13 1.79 -1.43 -2.92 -0.61 -2.10 1.30 -1.98
Standard name
Human Ortholog
Description Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise the CDK-cyclin BUR kinase complex which is involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (Rpo21p); BUR kinase is also involved in the recruitment of Spt6p to the CTD at the onset of transcription
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Bur2

Bur2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bur2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available