Standard name | |
---|---|
Human Ortholog | |
Description | Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud | 0 | 1 | 2 | 1 | 2 | 0 | 4 | 0 | 3 | 6 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 2 | 7 | 5 | 4 | 8 | 9 | 11 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 10 | 11 | 9 | 16 | 32 | 45 | 72 | 20 | 19 | 14 | 17 | 8 | 20 | 26 | 30 | 25 | 28 | 0 | 2 | 1 | 0 | 4 | 2 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 10 | 10 | 11 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 1 | 1 | 0 | 1 | 0 | 5 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 9 | 13 | 13 | 1 | 3 | 0 | 0 | 5 | 7 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 2 |
Mitochondria | 15 | 20 | 18 | 15 | 13 | 68 | 104 | 69 | 98 | 105 | 106 | 90 | 29 | 10 | 12 | 9 | 11 | 14 | 0 | 1 | 0 | 2 | 8 | 7 |
Nucleus | 80 | 170 | 115 | 107 | 122 | 177 | 231 | 201 | 111 | 85 | 94 | 122 | 128 | 147 | 150 | 153 | 170 | 142 | 87 | 168 | 112 | 59 | 93 | 61 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 2 | 0 | 4 | 4 | 4 | 3 | 4 | 5 | 7 | 1 | 1 | 5 | 1 | 1 | 1 | 2 | 6 | 1 | 1 | 8 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 5 | 6 | 4 | 2 | 6 | 14 | 1 | 1 | 5 | 6 | 0 | 0 | 1 | 7 | 11 | 8 | 1 | 5 | 4 | 1 | 4 | 4 |
Vac/Vac Membrane | 4 | 16 | 2 | 1 | 1 | 6 | 3 | 6 | 1 | 1 | 2 | 2 | 6 | 11 | 13 | 35 | 22 | 23 | 16 | 18 | 22 | 24 | 51 | 46 |
Unique Cell Count | 88 | 192 | 132 | 124 | 144 | 245 | 316 | 311 | 162 | 156 | 152 | 172 | 152 | 183 | 197 | 225 | 231 | 208 | 112 | 216 | 147 | 95 | 192 | 161 |
Labelled Cell Count | 105 | 222 | 155 | 144 | 161 | 299 | 411 | 375 | 241 | 232 | 237 | 260 | 173 | 192 | 208 | 257 | 264 | 244 | 112 | 216 | 147 | 95 | 192 | 161 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.0 | 4.8 | 4.8 | 5.4 | 4.5 | 4.7 | 5.4 | 3.9 | 3.9 | 3.6 | 3.8 | 4.7 | 4.6 | 4.8 | 6.9 | 6.9 | 6.7 | 4.8 | 4.6 | 4.9 |
Std Deviation (1e-4) | 0.8 | 1.0 | 1.0 | 1.3 | 1.5 | 1.3 | 1.8 | 2.2 | 1.5 | 1.4 | 1.3 | 1.1 | 1.2 | 1.2 | 1.1 | 2.0 | 1.8 | 1.5 | 1.2 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.18 | -0.08 | -0.02 | 0.17 | -0.29 | -0.3 | -0.4 | -0.32 | -0.02 | -0.06 | -0.01 | 0.52 | 0.53 | 0.49 | -0.01 | -0.06 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.3 | 0.8 | 1.4 | 1.7 | 3.7 | 1.1 | 1.1 | 0.3 | 0.5 | -1.0 | 0.8 | 1.4 | 1.4 | 0.8 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -1.2 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -3.0 | -2.1 |
Nucleus | -0.2 | -0.6 | -3.3 | -3.2 | -4.8 | -3.8 | -6.0 | -4.8 | -3.4 | -0.7 | -1.6 | -2.5 | -4.0 | -3.0 | -3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 2.2 | 4.2 | 3.0 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1777 | 2.9868 | 2.2385 | 2.2787 | 1.9154 | 2.4655 | 0.4251 | 0.4035 | 0.4158 | -0.0111 | 0.7218 | 0.1828 | 0.1233 | -0.047 | -0.1494 | -0.2786 | -0.5028 | -0.0489 |
Actin | 0.0139 | 0.0001 | 0.0023 | 0.0471 | 0.0013 | 0.0058 | 0.0196 | 0 | 0.0141 | 0.0009 | 0.0293 | 0.0016 | 0.0175 | 0.0082 | 0.0208 | 0.0003 | 0.0019 | 0.0089 |
Bud | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0008 | 0 | 0.0005 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 |
Bud Neck | 0.003 | 0.0007 | 0.0027 | 0.0021 | 0.0225 | 0.0029 | 0.0011 | 0.0003 | 0.0016 | 0.001 | 0.0018 | 0.0017 | 0.0014 | 0.0006 | 0.0022 | 0.0001 | 0.0008 | 0.0036 |
Bud Periphery | 0.0007 | 0 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0018 | 0 | 0.0007 | 0.0006 | 0.0035 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0003 |
Bud Site | 0.0024 | 0.0004 | 0.0009 | 0.0004 | 0.0011 | 0.0001 | 0.0062 | 0.0001 | 0.0041 | 0.0008 | 0.0029 | 0.0003 | 0.0003 | 0.0012 | 0.0009 | 0 | 0.0001 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0028 | 0.0194 | 0.0079 | 0.004 | 0.0029 | 0.0009 | 0.0048 | 0.005 | 0.017 | 0.0066 | 0.002 | 0.008 | 0.0091 | 0.0017 | 0.0028 | 0.0055 | 0.0049 | 0.0053 |
Cytoplasmic Foci | 0.0131 | 0.0004 | 0.0025 | 0.0005 | 0.0023 | 0.0011 | 0.0137 | 0.0001 | 0.0054 | 0.0023 | 0.0293 | 0.0035 | 0.0059 | 0.0002 | 0.0018 | 0.0001 | 0.0003 | 0.0005 |
Eisosomes | 0.0013 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0009 | 0.0101 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0025 | 0.0011 | 0.004 | 0.0079 | 0.0019 | 0.0015 | 0.0023 | 0.0003 | 0.0008 | 0.0005 | 0.0006 | 0.0009 |
Endosome | 0.0098 | 0.0018 | 0.0043 | 0.0008 | 0.0057 | 0.001 | 0.0187 | 0.0005 | 0.0166 | 0.0319 | 0.024 | 0.0092 | 0.006 | 0.0006 | 0.0032 | 0.0003 | 0.0022 | 0.0027 |
Golgi | 0.0043 | 0 | 0.002 | 0.0012 | 0.0052 | 0.0008 | 0.0083 | 0 | 0.0061 | 0.0059 | 0.0404 | 0.0034 | 0.0025 | 0.0005 | 0.0017 | 0 | 0.0001 | 0.0019 |
Lipid Particles | 0.0138 | 0.0002 | 0.0033 | 0.0009 | 0.0097 | 0.0065 | 0.0142 | 0 | 0.0037 | 0.0224 | 0.0321 | 0.0064 | 0.0123 | 0.0005 | 0.009 | 0.0001 | 0.0001 | 0.0023 |
Mitochondria | 0.005 | 0.0003 | 0.0094 | 0.0003 | 0.0277 | 0.0004 | 0.0079 | 0.0002 | 0.0127 | 0.038 | 0.0232 | 0.0053 | 0.0013 | 0.0005 | 0.0009 | 0.0002 | 0.0009 | 0.001 |
None | 0.0255 | 0.0171 | 0.0268 | 0.0581 | 0.002 | 0.0063 | 0.0032 | 0.0006 | 0.0111 | 0.0005 | 0.0012 | 0.0017 | 0.0136 | 0.0074 | 0.0102 | 0.0326 | 0.0043 | 0.0114 |
Nuclear Periphery | 0.0098 | 0.0215 | 0.0061 | 0.0023 | 0.0131 | 0.0018 | 0.0229 | 0.0135 | 0.0258 | 0.0269 | 0.0242 | 0.0059 | 0.0224 | 0.0067 | 0.0125 | 0.0089 | 0.0358 | 0.0083 |
Nucleolus | 0.0202 | 0.0326 | 0.0279 | 0.036 | 0.0361 | 0.0456 | 0.0167 | 0.0154 | 0.0126 | 0.006 | 0.0213 | 0.033 | 0.0159 | 0.0207 | 0.0163 | 0.004 | 0.0447 | 0.0287 |
Nucleus | 0.8377 | 0.8792 | 0.874 | 0.8323 | 0.849 | 0.9042 | 0.8308 | 0.9579 | 0.852 | 0.8289 | 0.7088 | 0.9049 | 0.8529 | 0.9285 | 0.9066 | 0.9443 | 0.8495 | 0.8969 |
Peroxisomes | 0.0119 | 0 | 0.0027 | 0.0008 | 0.0031 | 0.0037 | 0.0098 | 0 | 0.0013 | 0.0011 | 0.0315 | 0.0031 | 0.0103 | 0.0002 | 0.0014 | 0 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.021 | 0.0143 | 0.0248 | 0.0111 | 0.0162 | 0.0184 | 0.0119 | 0.0045 | 0.0081 | 0.0014 | 0.0109 | 0.0085 | 0.0248 | 0.0212 | 0.0071 | 0.0029 | 0.0526 | 0.0257 |
Vacuole | 0.0017 | 0.0013 | 0.0009 | 0.0003 | 0.0009 | 0.0001 | 0.0026 | 0.0005 | 0.0016 | 0.012 | 0.0055 | 0.0007 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.0008 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0021 | 0.0001 | 0.0011 | 0.0043 | 0.0044 | 0.0005 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.4105 | 13.7165 | 8.6814 | 11.8592 | 15.2131 | 8.1187 | 13.3588 | 12.9394 | 10.7781 | 17.0349 |
Translational Efficiency | 0.7946 | 0.8633 | 0.8613 | 0.5505 | 0.645 | 0.9402 | 0.6382 | 0.6423 | 0.7266 | 0.5873 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1707 | 1091 | 145 | 910 | 2071 | 1524 | 1939 | 238 | 3778 | 2615 | 2084 | 1148 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 688.89 | 781.75 | 891.40 | 871.71 | 726.79 | 782.36 | 808.10 | 887.26 | 709.67 | 782.11 | 813.90 | 874.93 |
Standard Deviation | 69.78 | 112.32 | 99.89 | 108.32 | 86.60 | 98.68 | 87.14 | 117.04 | 81.65 | 104.58 | 90.60 | 110.36 |
Intensity Change Log 2 | — | 0.182434 | 0.371799 | 0.339575 | — | 0.106294 | 0.152995 | 0.287818 | — | 0.143847 | 0.263615 | 0.313236 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000055 | 0.000432 | 0.000913 | 0.000866 | 0.000070 | 0.000292 | 0.000078 | 0.000896 | 0.000063 | 0.000350 | 0.000136 | 0.000872 |
Bud Neck | 0.001913 | 0.005296 | 0.006515 | 0.013768 | 0.003222 | 0.004923 | 0.002420 | 0.006704 | 0.002630 | 0.005079 | 0.002705 | 0.012303 |
Bud Site | 0.001590 | 0.001214 | 0.001141 | 0.008245 | 0.000875 | 0.002297 | 0.001242 | 0.007324 | 0.001198 | 0.001845 | 0.001235 | 0.008054 |
Cell Periphery | 0.000082 | 0.000099 | 0.000053 | 0.000140 | 0.000082 | 0.000116 | 0.000338 | 0.000197 | 0.000082 | 0.000109 | 0.000319 | 0.000152 |
Cytoplasm | 0.060323 | 0.028385 | 0.001746 | 0.043756 | 0.026386 | 0.038842 | 0.059284 | 0.121038* | 0.041719 | 0.034479 | 0.055280 | 0.059778 |
Cytoplasmic Foci | 0.009935 | 0.007125 | 0.000081 | 0.002664 | 0.002602 | 0.011197 | 0.003654 | 0.006428 | 0.005916 | 0.009498 | 0.003406 | 0.003444 |
Eisosomes | 0.000024 | 0.000032 | 0.000039 | 0.000035 | 0.000015 | 0.000034 | 0.000048 | 0.000065 | 0.000019 | 0.000033 | 0.000048 | 0.000041 |
Endoplasmic Reticulum | 0.000907 | 0.004503 | 0.010062 | 0.009433 | 0.001177 | 0.003219 | 0.000936 | 0.005937 | 0.001055 | 0.003755 | 0.001571 | 0.008708 |
Endosome | 0.000511 | 0.001037 | 0.000354 | 0.004143 | 0.000352 | 0.001737 | 0.000327 | 0.007731 | 0.000424 | 0.001445 | 0.000329 | 0.004887 |
Golgi | 0.000157 | 0.000485 | 0.000059 | 0.002145 | 0.000059 | 0.001437 | 0.000035 | 0.000387 | 0.000104 | 0.001040 | 0.000037 | 0.001780 |
Lipid Particles | 0.003714 | 0.002641 | 0.000379 | 0.000770 | 0.000955 | 0.004230 | 0.000619 | 0.001985 | 0.002201 | 0.003567 | 0.000603 | 0.001022 |
Mitochondria | 0.002828 | 0.003179 | 0.001080 | 0.002988 | 0.000955 | 0.007484 | 0.000618 | 0.001355 | 0.001801 | 0.005688 | 0.000651 | 0.002649 |
Mitotic Spindle | 0.000917 | 0.007677 | 0.006011 | 0.032055 | 0.001185 | 0.005277 | 0.001086 | 0.013121 | 0.001064 | 0.006278 | 0.001429 | 0.028130 |
None | 0.010637 | 0.007790 | 0.001306 | 0.007738 | 0.006340 | 0.008521 | 0.018355 | 0.027685 | 0.008281 | 0.008216 | 0.017169 | 0.011873 |
Nuclear Periphery | 0.001645 | 0.002022 | 0.000916 | 0.003302 | 0.001548 | 0.004211 | 0.000617 | 0.001880 | 0.001592 | 0.003298 | 0.000638 | 0.003007 |
Nuclear Periphery Foci | 0.000389 | 0.001249 | 0.000255 | 0.002501 | 0.000355 | 0.001025 | 0.000092 | 0.001874 | 0.000370 | 0.001119 | 0.000103 | 0.002371 |
Nucleolus | 0.014868 | 0.014569 | 0.003473 | 0.004287 | 0.013058 | 0.020906 | 0.003000 | 0.005444 | 0.013876 | 0.018262 | 0.003033 | 0.004527 |
Nucleus | 0.886381* | 0.904394* | 0.961300* | 0.844104* | 0.939639* | 0.875262* | 0.904646* | 0.759662* | 0.915576* | 0.887416* | 0.908588* | 0.826598* |
Peroxisomes | 0.001239 | 0.002812 | 0.000207 | 0.000425 | 0.000396 | 0.004105 | 0.000168 | 0.000565 | 0.000777 | 0.003565 | 0.000171 | 0.000454 |
Vacuole | 0.001407 | 0.003951 | 0.003993 | 0.014183 | 0.000627 | 0.004288 | 0.002347 | 0.028512 | 0.000979 | 0.004148 | 0.002462 | 0.017153 |
Vacuole Periphery | 0.000477 | 0.001109 | 0.000120 | 0.002452 | 0.000102 | 0.000597 | 0.000088 | 0.001211 | 0.000271 | 0.000811 | 0.000090 | 0.002195 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.37 | -7.68 | -13.14 | -7.24 | -0.06 | -5.09 | -2.45 | -9.76 | -7.93 | -9.40 | -8.15 | -6.49 | -15.71 | -11.00 | -13.68 |
Bud Neck | -5.79 | -5.29 | -9.78 | -6.48 | -3.30 | -2.60 | 0.79 | -6.54 | -5.58 | -6.99 | -5.83 | -1.71 | -10.73 | -8.36 | -10.20 |
Bud Site | -0.04 | -0.91 | -4.80 | -5.45 | -4.61 | -1.75 | -0.67 | -4.68 | -3.39 | -4.24 | -1.43 | -0.35 | -6.47 | -5.63 | -6.00 |
Cell Periphery | -1.60 | 0.65 | -6.13 | -2.98 | -5.99 | -1.74 | -5.18 | -4.29 | -2.54 | 3.11 | -2.28 | -5.35 | -6.06 | -3.09 | 3.67 |
Cytoplasm | 7.86 | 18.32 | 4.39 | -3.34 | -13.18 | -4.08 | -9.51 | -4.32 | -2.71 | -0.59 | 2.78 | -3.77 | -1.20 | -3.23 | 1.98 |
Cytoplasmic Foci | 2.21 | 10.36 | 8.74 | 4.02 | -4.88 | -6.91 | -1.40 | -0.80 | 5.54 | 0.05 | -3.04 | 5.09 | 7.69 | 8.17 | 3.01 |
Eisosomes | -3.29 | -4.15 | -11.04 | -7.38 | -1.72 | -9.02 | -5.64 | -9.71 | -7.61 | -5.71 | -8.59 | -5.31 | -15.63 | -10.72 | -4.56 |
Endoplasmic Reticulum | -7.68 | -9.47 | -20.17 | -10.75 | -1.07 | -6.95 | -0.44 | -12.41 | -10.28 | -12.25 | -10.32 | -5.41 | -23.29 | -16.38 | -20.61 |
Endosome | -2.93 | -0.38 | -5.15 | -3.71 | -5.03 | -4.52 | -0.33 | -2.83 | -1.71 | -2.81 | -5.28 | 0.78 | -5.96 | -3.36 | -6.07 |
Golgi | -2.68 | 1.56 | -1.55 | -0.70 | -1.73 | -3.20 | 2.63 | -4.06 | 2.60 | -4.45 | -3.66 | 3.33 | -1.83 | 0.82 | -2.04 |
Lipid Particles | 1.21 | 5.67 | 4.44 | 2.02 | -4.23 | -4.63 | 2.47 | -3.03 | 2.87 | -4.09 | -2.35 | 5.89 | 2.99 | 4.26 | -5.48 |
Mitochondria | -0.39 | 2.28 | 0.25 | 0.69 | -3.99 | -7.18 | 1.46 | -3.32 | 6.47 | -3.75 | -5.69 | 3.26 | -1.73 | 4.99 | -6.16 |
Mitotic Spindle | -3.01 | -0.90 | -7.09 | -4.96 | -4.17 | -3.19 | -0.38 | -3.64 | -3.12 | -3.61 | -4.38 | -0.83 | -7.96 | -6.26 | -7.75 |
None | 2.46 | 9.41 | 4.21 | 1.62 | -7.24 | -1.87 | -6.38 | -2.01 | -1.16 | 1.73 | 0.26 | -4.87 | 1.33 | 1.02 | 5.43 |
Nuclear Periphery | -1.82 | 0.94 | -8.87 | -6.87 | -9.25 | -3.39 | 5.18 | -6.97 | -0.42 | -9.38 | -3.78 | 6.15 | -10.74 | -3.69 | -13.58 |
Nuclear Periphery Foci | -2.55 | 0.40 | -4.44 | -2.71 | -4.49 | -2.89 | 2.00 | -4.07 | -2.58 | -4.40 | -3.84 | 2.94 | -5.31 | -3.53 | -5.69 |
Nucleolus | 0.33 | 8.26 | 6.08 | 5.82 | -1.80 | -4.54 | 9.17 | 2.01 | 4.95 | -2.24 | -3.37 | 11.90 | 6.82 | 9.07 | -2.72 |
Nucleus | -2.24 | -7.45 | 7.01 | 8.35 | 12.13 | 10.70 | 6.94 | 11.09 | 7.02 | 9.07 | 6.20 | 1.22 | 14.43 | 9.69 | 13.00 |
Peroxisomes | -2.12 | 4.01 | 3.50 | 3.54 | -1.74 | -5.01 | 2.79 | -1.20 | 4.72 | -3.07 | -5.23 | 4.88 | 2.93 | 6.09 | -3.69 |
Vacuole | -3.02 | -4.20 | -12.35 | -10.59 | -8.83 | -5.21 | -5.59 | -7.63 | -7.28 | -7.29 | -5.68 | -5.82 | -14.13 | -12.86 | -12.88 |
Vacuole Periphery | -0.96 | 1.00 | -1.10 | 0.16 | -2.43 | -6.93 | 0.37 | -1.69 | 0.50 | -1.72 | -1.96 | 1.21 | -1.99 | -0.20 | -2.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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