Standard name
Human Ortholog
Description Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.97 0.91 0.8 0.7 0.72 0.67 0.64 0.59 0.55 0.99 0.99 0.98 0.98 0.97 1.0 0.89 0.88 0.78 0.91 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0.1 0.14 0.36 0.49 0.35 0.43 0.53 0.57 0.59 0 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 3 8 3 4 8
Bud 1 4 0 0 6 14 23 9 17 26 30 29 0 0 1 1 0 0 10 11 2 7 27 27
Bud Neck 1 1 2 0 3 3 1 2 3 4 1 3 1 0 3 1 0 0 1 2 2 1 2 3
Bud Site 0 0 0 0 0 0 4 2 2 3 11 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 12 3 2 1 4 6 4 2 2 9 3 3 1 3 3 1 0 1 4 1 1 1
Cytoplasm 559 421 492 250 404 577 592 580 515 558 475 500 604 556 724 119 150 107 507 375 404 391 634 675
Endoplasmic Reticulum 3 1 1 0 1 2 0 2 1 1 2 2 1 2 6 3 20 4 1 1 6 1 2 4
Endosome 0 0 1 3 2 5 3 19 4 0 2 5 1 0 2 0 2 0 6 2 10 4 5 8
Golgi 2 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 4 0 1 2
Mitochondria 31 2 7 25 61 260 414 280 336 457 458 537 0 0 1 1 1 2 12 7 50 2 9 14
Nucleus 0 2 1 0 6 5 4 5 6 13 9 6 1 0 1 3 1 4 0 1 0 2 1 0
Nuclear Periphery 2 1 0 0 0 1 1 0 0 2 3 12 0 1 0 0 1 0 1 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 2 0 3 1 2 0 0 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 1 0 0 0 2 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 1 0 0 4 1 4 14 20 17 6 14 15 0 0 0 0 0 0 8 6 8 3 18 26
Vac/Vac Membrane 4 1 4 3 6 19 24 36 44 46 66 60 3 4 7 1 1 0 2 2 2 2 8 6
Unique Cell Count 579 425 500 259 444 719 842 811 773 867 805 913 609 561 738 121 155 107 570 425 518 431 731 792
Labelled Cell Count 604 436 521 289 492 891 1086 965 949 1121 1074 1185 614 567 746 133 180 118 570 425 518 431 731 792


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 5.2 4.6 4.1 4.2 3.5 3.3 3.7 3.6 3.4 3.3 3.4 6.2 6.4 6.0 5.6 5.2 5.1 6.2 6.4 6.4
Std Deviation (1e-4) 0.9 1.9 1.2 1.6 2.1 1.5 1.3 1.6 1.4 1.2 1.3 1.3 1.6 1.8 1.8 1.5 1.2 1.3 1.8 1.9 1.6
Intensity Change (Log2) – – – -0.16 -0.11 -0.37 -0.46 -0.29 -0.35 -0.43 -0.49 -0.44 0.44 0.48 0.4 0.28 0.18 0.17 0.43 0.49 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 -2.7 -2.6 -3.8 0 0 0
Cytoplasm -1.7 -5.2 -9.5 -12.6 -12.2 -13.6 -14.4 -15.8 -17.1 1.2 1.0 -0.4 0 -1.3 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2.3 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.3 2.5 3.7 4.5 4.3 5.8 5.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9401 1.8622 2.2629 1.9344 1.4443 1.982 0.1998 0.6074 0.7118 0.4332 0.7854 0.7509 2.12 2.0332 1.7089 1.4481 2.4105 1.6085
Actin 0.0608 0.0005 0.0193 0.0169 0.0294 0.0002 0.0047 0.0187 0.0296 0.0001 0.0153 0.0085 0.0129 0.0005 0.0092 0.0073 0.0255 0.0057
Bud 0.0067 0.0012 0.0008 0.0005 0.0002 0.0001 0.0031 0.0012 0.0016 0 0.0002 0.0012 0.0023 0.0105 0.0013 0.0001 0.0064 0.0007
Bud Neck 0.0088 0.0002 0.002 0.0002 0.0017 0.0003 0.0007 0.0001 0.0018 0.0001 0.0003 0.0006 0.0069 0.0002 0.0002 0.0007 0.0049 0.0006
Bud Periphery 0.0037 0.0005 0.001 0.0006 0.0007 0.0003 0.0012 0.0032 0.0034 0 0.0002 0.0021 0.0042 0.0034 0.0019 0.0001 0.0065 0.0006
Bud Site 0.0051 0.0119 0.0033 0.0005 0.0002 0 0.0123 0.0021 0.0021 0 0.0005 0.0003 0.025 0.0118 0.0047 0.0032 0.0084 0.0001
Cell Periphery 0.0005 0.0003 0.0002 0.0001 0.0002 0.0001 0.0006 0.0005 0.0007 0 0 0.0001 0.0009 0.0003 0.0003 0.0001 0.0003 0
Cytoplasm 0.2378 0.2928 0.2919 0.2415 0.2238 0.3558 0.1645 0.1698 0.1685 0.2445 0.1376 0.3315 0.1938 0.3336 0.3264 0.2847 0.1866 0.379
Cytoplasmic Foci 0.0312 0.0066 0.0039 0.0075 0.0062 0.0098 0.0134 0.0049 0.0048 0.0024 0.0095 0.0079 0.0252 0.006 0.0155 0.0119 0.0439 0.009
Eisosomes 0.0012 0.0001 0.0002 0.0003 0.0011 0 0.0002 0.0003 0.0009 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0.0001 0.0003 0
Endoplasmic Reticulum 0.0043 0.0016 0.0021 0.0013 0.0025 0.0014 0.0025 0.0023 0.0017 0.0007 0.0061 0.0012 0.0057 0.0009 0.0025 0.0021 0.003 0.001
Endosome 0.0061 0.0012 0.0014 0.0061 0.0026 0.0014 0.013 0.0014 0.0049 0.0004 0.0138 0.0051 0.0325 0.001 0.0042 0.0014 0.0496 0.0011
Golgi 0.0041 0.0001 0.001 0.004 0.0014 0.0001 0.0035 0.001 0.0044 0 0.0121 0.0004 0.0085 0.0001 0.0015 0.0021 0.0273 0.0002
Lipid Particles 0.0048 0.0003 0.0008 0.0074 0.0007 0.0001 0.0164 0.0003 0.003 0.0001 0.0012 0.0002 0.0177 0.0002 0.0011 0.0035 0.019 0.0002
Mitochondria 0.0112 0.0002 0.0003 0.0065 0.0009 0.0001 0.003 0.0013 0.0105 0.0001 0.0091 0.0004 0.0104 0.001 0.0016 0.0008 0.0212 0.0003
None 0.582 0.6746 0.6653 0.6864 0.7159 0.625 0.7267 0.7892 0.754 0.7495 0.7833 0.6353 0.6127 0.616 0.6125 0.6559 0.5092 0.593
Nuclear Periphery 0.0026 0.0006 0.0008 0.0058 0.0053 0.0004 0.0029 0.0007 0.0009 0.0003 0.0016 0.0005 0.0051 0.0006 0.0024 0.0007 0.0274 0.001
Nucleolus 0.0014 0.0003 0.0002 0.0004 0.0005 0.0001 0.0023 0.0001 0.0002 0 0 0.0001 0.0014 0.0013 0.0002 0.0001 0.0012 0.0001
Nucleus 0.0077 0.0036 0.0037 0.0045 0.0025 0.0015 0.0112 0.0009 0.0036 0.0009 0.0007 0.0028 0.0033 0.0098 0.002 0.001 0.0034 0.0035
Peroxisomes 0.0104 0.0001 0.0004 0.003 0.0002 0.0001 0.0095 0.0005 0.0017 0 0.007 0.0003 0.0168 0.0003 0.0092 0.0233 0.0373 0.0007
Punctate Nuclear 0.0068 0.002 0.0007 0.0051 0.0029 0.0025 0.0048 0.0004 0.0007 0.0004 0.001 0.0007 0.0059 0.0015 0.0022 0.0008 0.0113 0.0027
Vacuole 0.0024 0.0013 0.0007 0.0009 0.0006 0.0005 0.0029 0.0011 0.0007 0.0002 0.0003 0.0008 0.0069 0.0009 0.0009 0.0003 0.0036 0.0003
Vacuole Periphery 0.0006 0.0001 0.0001 0.0006 0.0006 0.0001 0.0006 0.0001 0.0003 0 0.0001 0.0001 0.0014 0.0001 0.0002 0.0001 0.0037 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9368 7.9663 9.1357 10.6264 11.0289 9.7162 10.0684 11.8613 12.512 13.9119
Translational Efficiency 1.1722 1.5911 1.395 1.5432 1.1462 1.1046 1.2774 1.0516 1.043 0.9065

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2044 199 1738 1211 1846 2410 198 1083 3890 2609 1936 2294

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 677.33 786.01 1471.01 1039.03 676.27 708.75 1587.51 891.23 676.83 714.64 1482.92 969.25
Standard Deviation 80.49 114.86 297.72 268.14 93.76 95.75 365.20 147.47 87.04 99.48 307.34 231.66
Intensity Change Log 2 — 0.214689 1.118876 0.617307 — 0.067677 1.231094 0.398198 — 0.143112 1.176032 0.511994

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000258 0.000927 0.000195 0.002999 0.000085 0.000211 0.000553 0.000755 0.000176 0.000265 0.000232 0.001940
Bud Neck 0.020776 0.030080 0.001552 0.006172 0.008233 0.036566 0.002477 0.012740 0.014824 0.036071 0.001646 0.009273
Bud Site 0.010786 0.032399 0.003573 0.013972 0.004506 0.017988 0.005024 0.050153 0.007806 0.019087 0.003721 0.031053
Cell Periphery 0.000287 0.000190 0.000100 0.000339 0.000153 0.000179 0.000226 0.000143 0.000223 0.000179 0.000113 0.000246
Cytoplasm 0.476309* 0.328648* 0.546079* 0.473230* 0.552151* 0.504557* 0.419662* 0.471111* 0.512300* 0.491140* 0.533150* 0.472229*
Cytoplasmic Foci 0.227660* 0.237244* 0.002561 0.015821 0.144612 0.163050 0.009883 0.020130 0.188250* 0.168710 0.003310 0.017855
Eisosomes 0.000261 0.000162 0.000006 0.000072 0.000110 0.000109 0.000044 0.000037 0.000190 0.000113 0.000010 0.000056
Endoplasmic Reticulum 0.001346 0.003327 0.000518 0.002231 0.000933 0.000833 0.002537 0.001351 0.001150 0.001023 0.000724 0.001816
Endosome 0.010961 0.039984 0.000387 0.006016 0.003464 0.014287 0.003046 0.018841 0.007404 0.016247 0.000659 0.012071
Golgi 0.002916 0.002658 0.000140 0.002344 0.000644 0.002494 0.000219 0.011540 0.001838 0.002506 0.000148 0.006685
Lipid Particles 0.010508 0.006192 0.000332 0.001313 0.004690 0.005237 0.001541 0.000905 0.007747 0.005310 0.000455 0.001120
Mitochondria 0.005178 0.004860 0.000628 0.003099 0.002697 0.006437 0.000350 0.015392 0.004001 0.006317 0.000599 0.008903
Mitotic Spindle 0.001413 0.015812 0.012891 0.036705 0.002563 0.011618 0.002470 0.078002 0.001958 0.011938 0.011826 0.056201
None 0.005136 0.001451 0.014562 0.012650 0.004522 0.004691 0.007130 0.009740 0.004845 0.004444 0.013802 0.011276
Nuclear Periphery 0.000455 0.000756 0.000677 0.001624 0.000478 0.000511 0.001020 0.001044 0.000466 0.000529 0.000712 0.001350
Nuclear Periphery Foci 0.000581 0.001438 0.000892 0.002324 0.000419 0.000250 0.006052 0.000966 0.000504 0.000340 0.001420 0.001683
Nucleolus 0.001694 0.001960 0.000218 0.001021 0.000919 0.002186 0.000188 0.000591 0.001326 0.002169 0.000215 0.000818
Nucleus 0.170241* 0.113964 0.391410* 0.296077* 0.240165* 0.151196* 0.385158* 0.161844* 0.203423* 0.148356* 0.390770* 0.232705*
Peroxisomes 0.005664 0.029176 0.000063 0.000688 0.001708 0.007116 0.000188 0.002093 0.003786 0.008798 0.000076 0.001351
Vacuole 0.046290 0.145932 0.023068 0.118706 0.026234 0.068642 0.152045 0.131114 0.036773 0.074538 0.036259 0.124564
Vacuole Periphery 0.001279 0.002839 0.000147 0.002598 0.000713 0.001843 0.000187 0.011508 0.001011 0.001919 0.000152 0.006805

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.42 -1.45 -1.24 -0.09 -1.04 -4.20 -10.13 -5.89 -4.00 4.44 -1.95 -4.90 -1.83 -1.55 -1.02
Bud Neck -1.29 14.48 11.84 4.61 -4.30 -14.95 2.99 0.38 14.61 -2.09 -11.34 16.21 10.09 16.72 -6.97
Bud Site -4.02 4.73 1.35 4.53 -3.18 -8.30 -3.35 -6.44 -1.74 -4.92 -7.28 3.39 -4.51 1.81 -7.01
Cell Periphery 2.43 3.98 2.45 0.09 -2.06 -1.78 -5.79 3.75 5.60 6.79 2.19 2.57 3.24 1.79 1.29
Cytoplasm 8.01 2.75 11.49 -2.24 9.17 5.87 15.55 16.87 12.13 -5.13 3.20 11.00 19.96 14.64 8.16
Cytoplasmic Foci 0.42 48.04 46.84 14.63 -6.18 -3.19 33.95 32.78 37.49 -4.02 4.33 58.41 55.87 41.15 -10.41
Eisosomes 5.12 22.79 18.59 7.06 -12.16 0.24 8.47 11.39 10.64 -0.63 9.80 27.59 21.10 12.34 -18.36
Endoplasmic Reticulum -2.49 2.25 -4.95 0.89 -9.17 0.35 -5.08 -6.00 -6.31 2.43 0.41 0.19 -7.49 -6.46 -10.53
Endosome -5.03 13.92 10.12 6.77 -5.42 -13.04 2.37 -2.62 6.77 -3.50 -9.14 16.03 5.16 12.28 -7.14
Golgi 1.07 8.68 4.66 3.13 -1.34 -6.06 3.62 -2.66 -0.47 -3.04 -1.98 9.63 0.26 1.46 -3.46
Lipid Particles 3.67 15.62 14.50 5.23 -7.74 -0.85 5.19 8.51 9.18 5.49 4.50 17.74 16.69 9.77 -6.21
Mitochondria 0.70 6.90 4.98 2.82 -1.42 -4.13 3.38 -1.28 1.35 -3.17 -3.04 7.20 1.49 3.96 -3.34
Mitotic Spindle -2.31 -8.35 -9.18 -3.09 -5.00 -5.01 -1.85 -8.72 -6.14 -7.34 -6.08 -8.16 -12.66 -7.99 -7.21
None 4.17 -6.87 -3.76 -9.43 3.28 -0.26 -0.73 -2.13 -1.92 -1.44 0.59 -8.64 -4.24 -4.67 4.48
Nuclear Periphery -1.97 -13.61 -12.57 -6.27 -7.51 -0.48 -11.86 -13.44 -12.99 -2.03 -1.18 -17.54 -18.15 -16.22 -9.75
Nuclear Periphery Foci -2.41 -4.21 -5.98 -1.09 -3.38 1.03 -7.34 -5.12 -11.13 6.17 1.35 -7.97 -7.80 -10.99 0.21
Nucleolus -0.56 5.40 4.29 3.58 -3.90 -4.20 3.05 1.93 7.14 -2.52 -3.18 6.09 4.59 7.67 -4.02
Nucleus 4.20 -38.11 -18.88 -15.18 11.54 15.01 -7.81 0.82 -10.73 7.91 11.99 -35.64 -13.20 -21.77 19.48
Peroxisomes -3.76 13.24 12.73 4.76 -3.92 -10.04 8.39 4.11 12.03 -2.57 -7.05 15.46 13.04 12.73 -4.04
Vacuole -9.21 -6.18 -23.47 -3.35 -19.24 -17.09 -12.40 -29.01 -23.21 -0.12 -15.47 -13.37 -36.97 -27.75 -23.32
Vacuole Periphery -2.48 8.36 1.74 3.20 -1.96 -4.78 3.60 -2.65 -1.30 -3.30 -4.33 8.75 -1.73 0.27 -3.85
Standard name
Human Ortholog
Description Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
Localization
Cell Percentages cytoplasm (28%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bna5

Bna5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bna5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available