Standard name
Human Ortholog
Description Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.13 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0.1 0.09 0.05 0 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.05 0.12 0 0 0
Cytoplasm 0.78 0.84 0.77 0.8 0.82 0.86 0.78 0.81 0.76 0.74 0.61 0.65 0.77 0.85 0.9 0.87 0.23 0.33 0.12 0.29 0.3 0.35
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.13 0.07 0.05 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.18 0.21 0.32 0.43 0.52 0.42 0 0 0 0 0 0 0.08 0.1 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.06 0 0.13 0.18 0.09 0.11 0 0 0 0 0 0.09 0.05 0 0.09 0.33 0.28 0.24 0.33 0.36 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 17 4 12 4 0 1 0 4 2 0 3 2 33 26 19 4 18 10 24 16 10 13
Bud 6 5 7 5 3 9 9 18 20 28 30 20 12 14 10 2 35 15 9 12 17 18
Bud Neck 0 0 1 0 0 0 0 4 1 3 3 2 9 4 6 3 24 13 15 10 8 11
Bud Site 1 0 1 0 0 0 1 2 2 4 3 2 1 1 0 0
Cell Periphery 27 12 17 3 1 2 5 5 2 1 7 6 22 27 43 2 28 8 21 10 12 8
Cytoplasm 249 141 134 152 196 307 301 424 310 312 248 294 527 715 827 180 84 57 22 83 90 141
Endoplasmic Reticulum 13 10 6 2 1 1 2 1 1 1 1 0 37 21 25 8 18 10 23 20 15 30
Endosome 1 0 2 5 2 2 3 7 2 0 1 4 6 5 1 2 4 2 2 5 4 2
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 1 2
Mitochondria 4 2 3 3 5 48 69 110 132 179 213 190 2 2 0 9 10 5 14 28 22 21
Nucleus 5 1 0 8 9 5 8 3 4 1 4 2 11 15 11 8 0 0 0 0 0 1
Nuclear Periphery 1 0 0 2 0 1 2 4 7 3 1 1 0 0 0 1 1 0 0 0 0 0
Nucleolus 0 1 1 0 0 0 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 4 0 0 0 2 4 2 1 1 0 0 3 0 0 0 0 0 0 0 1
SpindlePole 2 0 5 3 0 0 6 5 9 9 14 12 19 16 5 1 7 0 1 1 2 4
Vac/Vac Membrane 27 10 7 24 43 31 43 23 11 13 10 20 63 46 17 19 119 50 44 94 111 134
Unique Cell Count 320 168 175 190 238 355 384 526 407 421 407 449 684 840 916 207 359 177 186 289 306 399
Labelled Cell Count 354 186 200 212 260 407 451 615 507 555 539 555 745 895 964 239 359 177 186 289 306 399


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.1 5.8 5.8 5.0 3.9 3.6 3.6 3.3 3.0 3.2 3.1 6.5 6.0 6.0 4.6 4.9 5.6 5.8 6.1 6.1
Std Deviation (1e-4) 2.5 2.4 3.6 3.2 2.5 2.2 1.7 1.3 1.2 1.1 1.3 1.3 2.7 1.7 1.5 1.0 1.0 1.3 2.0 2.2 1.9
Intensity Change (Log2) -0.01 -0.23 -0.59 -0.7 -0.71 -0.81 -0.95 -0.88 -0.9 0.15 0.04 0.03 -0.34 -0.27 -0.07 -0.02 0.06 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -4.1 0 0 0 0 0 0 0 -1.1 -2.4 0 -2.4
Bud -0.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.4 -4.6 0 0 0 0 0 0 0 -3.7 -3.8 -2.7 -3.9
Cytoplasm 0.8 1.4 2.9 0.5 1.1 -0.1 -0.6 -3.6 -2.7 0.1 2.8 5.1 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.1 0 0 0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 1.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 4.3 2.0 2.8 0.2 -0.8 -0.6 -1.0 0.3 2.2 0.8 0 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1055 2.1146 2.0811 1.483 4.1854 2.1564 3.2177 3.8705 3.548 3.0047 2.5398 3.6719 3.5782 5.0001 3.4976 3.7479 3.5477 3.83
Actin 0.0646 0.0023 0.0031 0.002 0.0431 0.0098 0.0242 0.0039 0.0106 0.0001 0.002 0.0079 0.0266 0.0006 0.0064 0.0015 0.0095 0.0041
Bud 0.0033 0.0019 0.0163 0.0196 0.0068 0.006 0.0052 0.0133 0.0049 0.0022 0.009 0.0052 0.0058 0.0019 0.0072 0.005 0.0004 0.0038
Bud Neck 0.0036 0.0022 0.0057 0.0119 0.0062 0.0081 0.0031 0.0041 0.0043 0.0058 0.0056 0.0269 0.0032 0.0076 0.0082 0.0031 0.0026 0.0313
Bud Periphery 0.0145 0.0034 0.0545 0.0488 0.0213 0.0439 0.0173 0.0316 0.0271 0.006 0.034 0.0383 0.0267 0.0047 0.0156 0.0744 0.0008 0.0127
Bud Site 0.0138 0.047 0.0072 0.0102 0.0095 0.0032 0.0149 0.0855 0.0095 0.0049 0.0085 0.0024 0.0077 0.0492 0.0236 0.0015 0.0014 0.0026
Cell Periphery 0.0323 0.0151 0.0167 0.0259 0.0454 0.0305 0.0281 0.0282 0.0366 0.0108 0.0097 0.0213 0.0342 0.0485 0.0337 0.0154 0.0041 0.023
Cytoplasm 0.1651 0.2435 0.232 0.181 0.1341 0.2318 0.1659 0.1684 0.196 0.1111 0.172 0.1783 0.2358 0.2708 0.2193 0.1141 0.2162 0.2216
Cytoplasmic Foci 0.0741 0.0468 0.0262 0.0402 0.0432 0.0284 0.0557 0.0328 0.0573 0.0624 0.047 0.0808 0.0461 0.0711 0.0426 0.1415 0.0333 0.0547
Eisosomes 0.0008 0.0003 0.0004 0.0001 0.0001 0.0002 0.0008 0.0001 0.0017 0 0.001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0133 0.0079 0.0146 0.0053 0.0108 0.016 0.0215 0.0051 0.0167 0.0063 0.0066 0.0086 0.0137 0.0093 0.0107 0.0053 0.0114 0.0102
Endosome 0.08 0.0884 0.1158 0.2345 0.1719 0.1627 0.1338 0.0943 0.1105 0.1624 0.1026 0.1446 0.133 0.1007 0.1303 0.2256 0.146 0.166
Golgi 0.0078 0.0023 0.0108 0.0038 0.0161 0.0082 0.0123 0.0025 0.0086 0.0062 0.0029 0.0067 0.0121 0.0066 0.0076 0.01 0.0199 0.0071
Lipid Particles 0.02 0.0144 0.0052 0.0108 0.0214 0.0076 0.0635 0.0221 0.0331 0.0186 0.0314 0.0482 0.0738 0.0277 0.0361 0.0808 0.0442 0.0412
Mitochondria 0.0039 0.0027 0.0066 0.0029 0.0097 0.0168 0.0204 0.0027 0.0053 0.0011 0.0017 0.0095 0.0053 0.0044 0.0087 0.0015 0.0035 0.0077
None 0.3139 0.3414 0.2612 0.1486 0.0058 0.2209 0.202 0.2054 0.2497 0.1509 0.1981 0.1942 0.1775 0.1221 0.2001 0.1075 0.1824 0.158
Nuclear Periphery 0.0131 0.0028 0.0045 0.0031 0.0037 0.0075 0.0205 0.0014 0.0027 0.0014 0.002 0.0027 0.003 0.0074 0.0061 0.001 0.0025 0.0025
Nucleolus 0.0034 0.0055 0.0012 0.0026 0.0012 0.0018 0.0036 0.0058 0.0025 0.0041 0.0061 0.0016 0.0014 0.0017 0.0025 0.0009 0.0008 0.0012
Nucleus 0.0085 0.0043 0.0078 0.004 0.0036 0.0086 0.0054 0.0088 0.004 0.0049 0.0056 0.0033 0.0023 0.0035 0.0038 0.0018 0.0032 0.0025
Peroxisomes 0.0131 0.0011 0.0011 0.0012 0.0082 0.0012 0.0058 0.0009 0.0028 0.0025 0.0022 0.004 0.0022 0.0039 0.0035 0.0024 0.0058 0.0015
Punctate Nuclear 0.0338 0.0013 0.0006 0.0014 0.001 0.0089 0.0042 0.0026 0.0017 0.0005 0.0008 0.002 0.0011 0.0011 0.0029 0.0019 0.0008 0.0007
Vacuole 0.1118 0.1583 0.1927 0.2216 0.4104 0.1686 0.1732 0.272 0.2066 0.4235 0.3379 0.2019 0.1792 0.2343 0.213 0.1937 0.301 0.2262
Vacuole Periphery 0.0053 0.0069 0.0157 0.0204 0.0269 0.0095 0.0184 0.0083 0.0078 0.0143 0.0133 0.0114 0.0091 0.0226 0.0177 0.011 0.01 0.0211

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.8416 29.0918 42.7674 39.4704 27.4658 38.6622 35.3809 30.9031 39.0081 35.4521
Translational Efficiency 0.2468 0.5128 0.627 0.5728 0.3987 0.2217 0.3942 0.4065 0.372 0.2571

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
116 391 231 1593 1210 1618 1898 1851 1326 2009 2129 3444

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1123.40 737.22 6754.08 1351.35 696.56 787.61 1025.23 1280.07 733.90 777.80 1646.82 1313.04
Standard Deviation 229.89 138.66 2280.62 524.46 126.41 142.75 264.60 502.89 183.71 143.36 1949.71 514.21
Intensity Change Log 2 -0.607705 2.587888 0.266530 0.177234 0.557628 0.877903 -0.255258 2.095735 0.531935

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002010 0.002612 0.000492 0.024594 0.001924 0.004857 0.023615 0.024538 0.001931 0.004420 0.021106 0.024564
Bud Neck 0.016571 0.070449 0.002554 0.026835 0.084586 0.089844 0.059630 0.027010 0.078636 0.086069 0.053437 0.026929
Bud Site 0.046480 0.112038* 0.015374 0.235155* 0.100700* 0.109525* 0.262980* 0.239481* 0.095956 0.110014* 0.236114* 0.237480*
Cell Periphery 0.015639 0.016415 0.790442* 0.477463* 0.029262 0.040511 0.457680* 0.406685* 0.028070 0.035821 0.493786* 0.439423*
Cytoplasm 0.127680* 0.321004* 0.008234 0.142592* 0.341235* 0.350657* 0.139115* 0.224909* 0.322553* 0.344886* 0.124914* 0.186834*
Cytoplasmic Foci 0.043610 0.168942 0.000222 0.004996 0.135169 0.116957 0.001733 0.005074 0.127160 0.127075 0.001569 0.005038
Eisosomes 0.000850 0.000973 0.008634 0.007912 0.002517 0.003152 0.009316 0.006031 0.002371 0.002728 0.009242 0.006901
Endoplasmic Reticulum 0.010217 0.004717 0.000813 0.005007 0.003793 0.005526 0.006852 0.003444 0.004355 0.005369 0.006197 0.004167
Endosome 0.028230 0.044046 0.000273 0.002394 0.030970 0.027397 0.000503 0.002790 0.030730 0.030637 0.000478 0.002607
Golgi 0.021626 0.003753 0.001237 0.003741 0.003811 0.007331 0.000325 0.002147 0.005369 0.006635 0.000424 0.002884
Lipid Particles 0.001292 0.008273 0.005249 0.004060 0.009633 0.007362 0.001846 0.004490 0.008904 0.007539 0.002215 0.004291
Mitochondria 0.001861 0.006884 0.000153 0.002530 0.004181 0.005828 0.001548 0.001757 0.003978 0.006033 0.001397 0.002115
Mitotic Spindle 0.002822 0.003207 0.000910 0.002931 0.000549 0.001149 0.000194 0.001138 0.000748 0.001549 0.000272 0.001968
None 0.000107 0.003595 0.000039 0.000331 0.000895 0.001176 0.000342 0.001024 0.000826 0.001647 0.000309 0.000704
Nuclear Periphery 0.000461 0.000451 0.002986 0.000308 0.000190 0.000254 0.000101 0.000143 0.000214 0.000292 0.000414 0.000219
Nuclear Periphery Foci 0.001088 0.000766 0.000332 0.000586 0.000279 0.000353 0.000312 0.000679 0.000350 0.000433 0.000314 0.000636
Nucleolus 0.000224 0.001230 0.000092 0.000060 0.000493 0.000418 0.000040 0.000030 0.000469 0.000576 0.000045 0.000044
Nucleus 0.022557 0.034256 0.001563 0.006905 0.038236 0.041123 0.014097 0.007230 0.036865 0.039787 0.012737 0.007079
Peroxisomes 0.002013 0.013881 0.000021 0.000914 0.005750 0.010062 0.000998 0.000851 0.005423 0.010806 0.000892 0.000880
Vacuole 0.633343* 0.179754 0.156418 0.048954 0.203655* 0.174175 0.018549 0.039811 0.241245* 0.175261 0.033508 0.044040
Vacuole Periphery 0.021317 0.002754 0.003963 0.001732 0.002172 0.002343 0.000226 0.000738 0.003847 0.002423 0.000631 0.001198

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.56 1.34 -12.05 -13.48 -15.31 -3.47 -15.53 -13.94 -11.23 -0.29 -3.49 -15.23 -19.35 -15.79 -1.91
Bud Neck -7.54 4.48 -3.27 6.39 -13.99 -0.92 4.90 12.36 15.75 11.45 -1.39 5.38 12.27 17.26 10.84
Bud Site -4.31 2.26 -15.45 -9.41 -21.83* -1.06 -17.71 -15.21 -15.26 2.62 -1.79 -16.51 -18.47 -18.46 -0.01
Cell Periphery -0.22 -54.50* -37.57* -40.76* 20.42 -2.33 -46.53* -38.50* -37.28* 4.51 -1.82 -53.76 -54.09 -53.75* 6.10
Cytoplasm -7.96 6.91 -0.61 11.69 -21.13 -0.91 22.30 11.40 13.20 -11.50 -2.25 23.16 15.31 20.35 -11.10
Cytoplasmic Foci -9.79 5.04 4.40 16.94 -6.72 2.75 25.95 25.18 26.62 -6.35 -0.00 26.14 25.26 31.25 -7.62
Eisosomes -0.35 -4.04 -9.12 -10.84 0.48 -1.37 -9.26 -5.50 -4.41 3.91 -0.90 -9.97 -8.85 -8.28 3.33
Endoplasmic Reticulum 0.85 1.63 0.84 -0.18 -6.60 -1.67 -3.46 0.39 2.15 4.19 -1.00 -2.17 0.14 1.39 3.06
Endosome -2.91 5.91 5.41 9.64 -5.37 1.56 16.67 14.84 15.49 -4.30 0.02 17.57 16.04 18.51 -6.30
Golgi 1.66 1.92 1.66 0.04 -2.41 -3.72 9.23 2.63 5.14 -3.45 -1.26 5.27 2.24 4.26 -4.46
Lipid Particles -5.09 -3.23 -3.63 2.75 0.86 2.47 8.44 4.60 3.01 -2.70 1.63 7.88 5.17 4.44 -2.83
Mitochondria -2.08 1.36 -0.59 1.91 -3.55 -1.85 4.44 3.69 5.44 -0.47 -2.35 4.67 2.98 5.13 -1.74
Mitotic Spindle -0.21 1.18 -0.37 -0.04 -2.39 -1.09 0.96 -1.18 0.20 -2.83 -1.27 1.26 -2.29 -0.59 -3.85
None -2.25 1.44 -1.59 2.08 -2.25 -0.62 2.74 -0.19 0.23 -1.21 -1.73 2.82 0.39 1.76 -1.26
Nuclear Periphery 0.04 -7.19 0.81 0.40 8.42 -0.70 4.33 1.68 1.23 -1.80 -0.80 -4.40 -0.20 0.69 3.74
Nuclear Periphery Foci 0.97 3.64 2.30 1.27 -3.94 -1.05 -0.88 -1.31 -1.05 -1.20 -1.38 1.07 -1.75 -1.11 -2.00
Nucleolus -3.79 2.32 2.77 4.48 1.48 0.94 6.58 6.70 6.37 2.60 -1.26 6.73 6.70 7.35 0.02
Nucleus -1.05 2.45 1.68 7.08 -5.43 -1.10 10.02 15.23 16.72 4.64 -1.13 10.41 14.60 18.09 3.94
Peroxisomes -6.54 3.81 1.82 7.30 -3.40 -4.00 8.69 9.51 9.77 0.75 -5.60 9.04 9.38 11.80 0.13
Vacuole 13.77 14.77 18.62 10.72 9.48 3.09 24.37 20.10 20.13 -8.32 6.81 25.13 22.94 22.31 -5.76
Vacuole Periphery 2.13 1.96 2.25 1.59 4.06 -0.47 8.90 5.64 5.13 -3.76 1.66 4.21 3.33 3.83 -2.58
Standard name
Human Ortholog
Description Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
Localization
Cell Percentages vacuole (30%), cell periphery (8%), mixed (15%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Thi7

Thi7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thi7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available