Standard name | |
---|---|
Human Ortholog | |
Description | Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 4 | 12 | 4 | 0 | 1 | 0 | 4 | 2 | 0 | 3 | 2 | 33 | 26 | 19 | – | – | 4 | 18 | 10 | 24 | 16 | 10 | 13 |
Bud | 6 | 5 | 7 | 5 | 3 | 9 | 9 | 18 | 20 | 28 | 30 | 20 | 12 | 14 | 10 | – | – | 2 | 35 | 15 | 9 | 12 | 17 | 18 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 3 | 3 | 2 | 9 | 4 | 6 | – | – | 3 | 24 | 13 | 15 | 10 | 8 | 11 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 4 | 3 | 2 | 1 | 1 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 27 | 12 | 17 | 3 | 1 | 2 | 5 | 5 | 2 | 1 | 7 | 6 | 22 | 27 | 43 | – | – | 2 | 28 | 8 | 21 | 10 | 12 | 8 |
Cytoplasm | 249 | 141 | 134 | 152 | 196 | 307 | 301 | 424 | 310 | 312 | 248 | 294 | 527 | 715 | 827 | – | – | 180 | 84 | 57 | 22 | 83 | 90 | 141 |
Endoplasmic Reticulum | 13 | 10 | 6 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 37 | 21 | 25 | – | – | 8 | 18 | 10 | 23 | 20 | 15 | 30 |
Endosome | 1 | 0 | 2 | 5 | 2 | 2 | 3 | 7 | 2 | 0 | 1 | 4 | 6 | 5 | 1 | – | – | 2 | 4 | 2 | 2 | 5 | 4 | 2 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 4 | 2 | 3 | 3 | 5 | 48 | 69 | 110 | 132 | 179 | 213 | 190 | 2 | 2 | 0 | – | – | 9 | 10 | 5 | 14 | 28 | 22 | 21 |
Nucleus | 5 | 1 | 0 | 8 | 9 | 5 | 8 | 3 | 4 | 1 | 4 | 2 | 11 | 15 | 11 | – | – | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 4 | 7 | 3 | 1 | 1 | 0 | 0 | 0 | – | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4 | 0 | 0 | 0 | 2 | 4 | 2 | 1 | 1 | 0 | 0 | 3 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 2 | 0 | 5 | 3 | 0 | 0 | 6 | 5 | 9 | 9 | 14 | 12 | 19 | 16 | 5 | – | – | 1 | 7 | 0 | 1 | 1 | 2 | 4 |
Vac/Vac Membrane | 27 | 10 | 7 | 24 | 43 | 31 | 43 | 23 | 11 | 13 | 10 | 20 | 63 | 46 | 17 | – | – | 19 | 119 | 50 | 44 | 94 | 111 | 134 |
Unique Cell Count | 320 | 168 | 175 | 190 | 238 | 355 | 384 | 526 | 407 | 421 | 407 | 449 | 684 | 840 | 916 | 207 | 359 | 177 | 186 | 289 | 306 | 399 | ||
Labelled Cell Count | 354 | 186 | 200 | 212 | 260 | 407 | 451 | 615 | 507 | 555 | 539 | 555 | 745 | 895 | 964 | 239 | 359 | 177 | 186 | 289 | 306 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.1 | 5.8 | 5.8 | 5.0 | 3.9 | 3.6 | 3.6 | 3.3 | 3.0 | 3.2 | 3.1 | 6.5 | 6.0 | 6.0 | 4.6 | 4.9 | 5.6 | 5.8 | 6.1 | 6.1 |
Std Deviation (1e-4) | 2.5 | 2.4 | 3.6 | 3.2 | 2.5 | 2.2 | 1.7 | 1.3 | 1.2 | 1.1 | 1.3 | 1.3 | 2.7 | 1.7 | 1.5 | 1.0 | 1.0 | 1.3 | 2.0 | 2.2 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.23 | -0.59 | -0.7 | -0.71 | -0.81 | -0.95 | -0.88 | -0.9 | 0.15 | 0.04 | 0.03 | -0.34 | -0.27 | -0.07 | -0.02 | 0.06 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.2 | -4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | -2.4 | 0 | – | – | -2.4 |
Bud | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | -3.4 | -4.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.7 | -3.8 | -2.7 | – | – | -3.9 |
Cytoplasm | 0.8 | 1.4 | 2.9 | 0.5 | 1.1 | -0.1 | -0.6 | -3.6 | -2.7 | 0.1 | 2.8 | 5.1 | – | – | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | – | – | 0.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 1.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | 3.0 | 4.3 | 2.0 | 2.8 | 0.2 | -0.8 | -0.6 | -1.0 | 0.3 | 2.2 | 0.8 | 0 | – | – | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1055 | 2.1146 | 2.0811 | 1.483 | 4.1854 | 2.1564 | 3.2177 | 3.8705 | 3.548 | 3.0047 | 2.5398 | 3.6719 | 3.5782 | 5.0001 | 3.4976 | 3.7479 | 3.5477 | 3.83 |
Actin | 0.0646 | 0.0023 | 0.0031 | 0.002 | 0.0431 | 0.0098 | 0.0242 | 0.0039 | 0.0106 | 0.0001 | 0.002 | 0.0079 | 0.0266 | 0.0006 | 0.0064 | 0.0015 | 0.0095 | 0.0041 |
Bud | 0.0033 | 0.0019 | 0.0163 | 0.0196 | 0.0068 | 0.006 | 0.0052 | 0.0133 | 0.0049 | 0.0022 | 0.009 | 0.0052 | 0.0058 | 0.0019 | 0.0072 | 0.005 | 0.0004 | 0.0038 |
Bud Neck | 0.0036 | 0.0022 | 0.0057 | 0.0119 | 0.0062 | 0.0081 | 0.0031 | 0.0041 | 0.0043 | 0.0058 | 0.0056 | 0.0269 | 0.0032 | 0.0076 | 0.0082 | 0.0031 | 0.0026 | 0.0313 |
Bud Periphery | 0.0145 | 0.0034 | 0.0545 | 0.0488 | 0.0213 | 0.0439 | 0.0173 | 0.0316 | 0.0271 | 0.006 | 0.034 | 0.0383 | 0.0267 | 0.0047 | 0.0156 | 0.0744 | 0.0008 | 0.0127 |
Bud Site | 0.0138 | 0.047 | 0.0072 | 0.0102 | 0.0095 | 0.0032 | 0.0149 | 0.0855 | 0.0095 | 0.0049 | 0.0085 | 0.0024 | 0.0077 | 0.0492 | 0.0236 | 0.0015 | 0.0014 | 0.0026 |
Cell Periphery | 0.0323 | 0.0151 | 0.0167 | 0.0259 | 0.0454 | 0.0305 | 0.0281 | 0.0282 | 0.0366 | 0.0108 | 0.0097 | 0.0213 | 0.0342 | 0.0485 | 0.0337 | 0.0154 | 0.0041 | 0.023 |
Cytoplasm | 0.1651 | 0.2435 | 0.232 | 0.181 | 0.1341 | 0.2318 | 0.1659 | 0.1684 | 0.196 | 0.1111 | 0.172 | 0.1783 | 0.2358 | 0.2708 | 0.2193 | 0.1141 | 0.2162 | 0.2216 |
Cytoplasmic Foci | 0.0741 | 0.0468 | 0.0262 | 0.0402 | 0.0432 | 0.0284 | 0.0557 | 0.0328 | 0.0573 | 0.0624 | 0.047 | 0.0808 | 0.0461 | 0.0711 | 0.0426 | 0.1415 | 0.0333 | 0.0547 |
Eisosomes | 0.0008 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0017 | 0 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0133 | 0.0079 | 0.0146 | 0.0053 | 0.0108 | 0.016 | 0.0215 | 0.0051 | 0.0167 | 0.0063 | 0.0066 | 0.0086 | 0.0137 | 0.0093 | 0.0107 | 0.0053 | 0.0114 | 0.0102 |
Endosome | 0.08 | 0.0884 | 0.1158 | 0.2345 | 0.1719 | 0.1627 | 0.1338 | 0.0943 | 0.1105 | 0.1624 | 0.1026 | 0.1446 | 0.133 | 0.1007 | 0.1303 | 0.2256 | 0.146 | 0.166 |
Golgi | 0.0078 | 0.0023 | 0.0108 | 0.0038 | 0.0161 | 0.0082 | 0.0123 | 0.0025 | 0.0086 | 0.0062 | 0.0029 | 0.0067 | 0.0121 | 0.0066 | 0.0076 | 0.01 | 0.0199 | 0.0071 |
Lipid Particles | 0.02 | 0.0144 | 0.0052 | 0.0108 | 0.0214 | 0.0076 | 0.0635 | 0.0221 | 0.0331 | 0.0186 | 0.0314 | 0.0482 | 0.0738 | 0.0277 | 0.0361 | 0.0808 | 0.0442 | 0.0412 |
Mitochondria | 0.0039 | 0.0027 | 0.0066 | 0.0029 | 0.0097 | 0.0168 | 0.0204 | 0.0027 | 0.0053 | 0.0011 | 0.0017 | 0.0095 | 0.0053 | 0.0044 | 0.0087 | 0.0015 | 0.0035 | 0.0077 |
None | 0.3139 | 0.3414 | 0.2612 | 0.1486 | 0.0058 | 0.2209 | 0.202 | 0.2054 | 0.2497 | 0.1509 | 0.1981 | 0.1942 | 0.1775 | 0.1221 | 0.2001 | 0.1075 | 0.1824 | 0.158 |
Nuclear Periphery | 0.0131 | 0.0028 | 0.0045 | 0.0031 | 0.0037 | 0.0075 | 0.0205 | 0.0014 | 0.0027 | 0.0014 | 0.002 | 0.0027 | 0.003 | 0.0074 | 0.0061 | 0.001 | 0.0025 | 0.0025 |
Nucleolus | 0.0034 | 0.0055 | 0.0012 | 0.0026 | 0.0012 | 0.0018 | 0.0036 | 0.0058 | 0.0025 | 0.0041 | 0.0061 | 0.0016 | 0.0014 | 0.0017 | 0.0025 | 0.0009 | 0.0008 | 0.0012 |
Nucleus | 0.0085 | 0.0043 | 0.0078 | 0.004 | 0.0036 | 0.0086 | 0.0054 | 0.0088 | 0.004 | 0.0049 | 0.0056 | 0.0033 | 0.0023 | 0.0035 | 0.0038 | 0.0018 | 0.0032 | 0.0025 |
Peroxisomes | 0.0131 | 0.0011 | 0.0011 | 0.0012 | 0.0082 | 0.0012 | 0.0058 | 0.0009 | 0.0028 | 0.0025 | 0.0022 | 0.004 | 0.0022 | 0.0039 | 0.0035 | 0.0024 | 0.0058 | 0.0015 |
Punctate Nuclear | 0.0338 | 0.0013 | 0.0006 | 0.0014 | 0.001 | 0.0089 | 0.0042 | 0.0026 | 0.0017 | 0.0005 | 0.0008 | 0.002 | 0.0011 | 0.0011 | 0.0029 | 0.0019 | 0.0008 | 0.0007 |
Vacuole | 0.1118 | 0.1583 | 0.1927 | 0.2216 | 0.4104 | 0.1686 | 0.1732 | 0.272 | 0.2066 | 0.4235 | 0.3379 | 0.2019 | 0.1792 | 0.2343 | 0.213 | 0.1937 | 0.301 | 0.2262 |
Vacuole Periphery | 0.0053 | 0.0069 | 0.0157 | 0.0204 | 0.0269 | 0.0095 | 0.0184 | 0.0083 | 0.0078 | 0.0143 | 0.0133 | 0.0114 | 0.0091 | 0.0226 | 0.0177 | 0.011 | 0.01 | 0.0211 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.8416 | 29.0918 | 42.7674 | 39.4704 | 27.4658 | 38.6622 | 35.3809 | 30.9031 | 39.0081 | 35.4521 |
Translational Efficiency | 0.2468 | 0.5128 | 0.627 | 0.5728 | 0.3987 | 0.2217 | 0.3942 | 0.4065 | 0.372 | 0.2571 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
116 | 391 | 231 | 1593 | 1210 | 1618 | 1898 | 1851 | 1326 | 2009 | 2129 | 3444 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1123.40 | 737.22 | 6754.08 | 1351.35 | 696.56 | 787.61 | 1025.23 | 1280.07 | 733.90 | 777.80 | 1646.82 | 1313.04 |
Standard Deviation | 229.89 | 138.66 | 2280.62 | 524.46 | 126.41 | 142.75 | 264.60 | 502.89 | 183.71 | 143.36 | 1949.71 | 514.21 |
Intensity Change Log 2 | — | -0.607705 | 2.587888 | 0.266530 | — | 0.177234 | 0.557628 | 0.877903 | — | -0.255258 | 2.095735 | 0.531935 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002010 | 0.002612 | 0.000492 | 0.024594 | 0.001924 | 0.004857 | 0.023615 | 0.024538 | 0.001931 | 0.004420 | 0.021106 | 0.024564 |
Bud Neck | 0.016571 | 0.070449 | 0.002554 | 0.026835 | 0.084586 | 0.089844 | 0.059630 | 0.027010 | 0.078636 | 0.086069 | 0.053437 | 0.026929 |
Bud Site | 0.046480 | 0.112038* | 0.015374 | 0.235155* | 0.100700* | 0.109525* | 0.262980* | 0.239481* | 0.095956 | 0.110014* | 0.236114* | 0.237480* |
Cell Periphery | 0.015639 | 0.016415 | 0.790442* | 0.477463* | 0.029262 | 0.040511 | 0.457680* | 0.406685* | 0.028070 | 0.035821 | 0.493786* | 0.439423* |
Cytoplasm | 0.127680* | 0.321004* | 0.008234 | 0.142592* | 0.341235* | 0.350657* | 0.139115* | 0.224909* | 0.322553* | 0.344886* | 0.124914* | 0.186834* |
Cytoplasmic Foci | 0.043610 | 0.168942 | 0.000222 | 0.004996 | 0.135169 | 0.116957 | 0.001733 | 0.005074 | 0.127160 | 0.127075 | 0.001569 | 0.005038 |
Eisosomes | 0.000850 | 0.000973 | 0.008634 | 0.007912 | 0.002517 | 0.003152 | 0.009316 | 0.006031 | 0.002371 | 0.002728 | 0.009242 | 0.006901 |
Endoplasmic Reticulum | 0.010217 | 0.004717 | 0.000813 | 0.005007 | 0.003793 | 0.005526 | 0.006852 | 0.003444 | 0.004355 | 0.005369 | 0.006197 | 0.004167 |
Endosome | 0.028230 | 0.044046 | 0.000273 | 0.002394 | 0.030970 | 0.027397 | 0.000503 | 0.002790 | 0.030730 | 0.030637 | 0.000478 | 0.002607 |
Golgi | 0.021626 | 0.003753 | 0.001237 | 0.003741 | 0.003811 | 0.007331 | 0.000325 | 0.002147 | 0.005369 | 0.006635 | 0.000424 | 0.002884 |
Lipid Particles | 0.001292 | 0.008273 | 0.005249 | 0.004060 | 0.009633 | 0.007362 | 0.001846 | 0.004490 | 0.008904 | 0.007539 | 0.002215 | 0.004291 |
Mitochondria | 0.001861 | 0.006884 | 0.000153 | 0.002530 | 0.004181 | 0.005828 | 0.001548 | 0.001757 | 0.003978 | 0.006033 | 0.001397 | 0.002115 |
Mitotic Spindle | 0.002822 | 0.003207 | 0.000910 | 0.002931 | 0.000549 | 0.001149 | 0.000194 | 0.001138 | 0.000748 | 0.001549 | 0.000272 | 0.001968 |
None | 0.000107 | 0.003595 | 0.000039 | 0.000331 | 0.000895 | 0.001176 | 0.000342 | 0.001024 | 0.000826 | 0.001647 | 0.000309 | 0.000704 |
Nuclear Periphery | 0.000461 | 0.000451 | 0.002986 | 0.000308 | 0.000190 | 0.000254 | 0.000101 | 0.000143 | 0.000214 | 0.000292 | 0.000414 | 0.000219 |
Nuclear Periphery Foci | 0.001088 | 0.000766 | 0.000332 | 0.000586 | 0.000279 | 0.000353 | 0.000312 | 0.000679 | 0.000350 | 0.000433 | 0.000314 | 0.000636 |
Nucleolus | 0.000224 | 0.001230 | 0.000092 | 0.000060 | 0.000493 | 0.000418 | 0.000040 | 0.000030 | 0.000469 | 0.000576 | 0.000045 | 0.000044 |
Nucleus | 0.022557 | 0.034256 | 0.001563 | 0.006905 | 0.038236 | 0.041123 | 0.014097 | 0.007230 | 0.036865 | 0.039787 | 0.012737 | 0.007079 |
Peroxisomes | 0.002013 | 0.013881 | 0.000021 | 0.000914 | 0.005750 | 0.010062 | 0.000998 | 0.000851 | 0.005423 | 0.010806 | 0.000892 | 0.000880 |
Vacuole | 0.633343* | 0.179754 | 0.156418 | 0.048954 | 0.203655* | 0.174175 | 0.018549 | 0.039811 | 0.241245* | 0.175261 | 0.033508 | 0.044040 |
Vacuole Periphery | 0.021317 | 0.002754 | 0.003963 | 0.001732 | 0.002172 | 0.002343 | 0.000226 | 0.000738 | 0.003847 | 0.002423 | 0.000631 | 0.001198 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.56 | 1.34 | -12.05 | -13.48 | -15.31 | -3.47 | -15.53 | -13.94 | -11.23 | -0.29 | -3.49 | -15.23 | -19.35 | -15.79 | -1.91 |
Bud Neck | -7.54 | 4.48 | -3.27 | 6.39 | -13.99 | -0.92 | 4.90 | 12.36 | 15.75 | 11.45 | -1.39 | 5.38 | 12.27 | 17.26 | 10.84 |
Bud Site | -4.31 | 2.26 | -15.45 | -9.41 | -21.83* | -1.06 | -17.71 | -15.21 | -15.26 | 2.62 | -1.79 | -16.51 | -18.47 | -18.46 | -0.01 |
Cell Periphery | -0.22 | -54.50* | -37.57* | -40.76* | 20.42 | -2.33 | -46.53* | -38.50* | -37.28* | 4.51 | -1.82 | -53.76 | -54.09 | -53.75* | 6.10 |
Cytoplasm | -7.96 | 6.91 | -0.61 | 11.69 | -21.13 | -0.91 | 22.30 | 11.40 | 13.20 | -11.50 | -2.25 | 23.16 | 15.31 | 20.35 | -11.10 |
Cytoplasmic Foci | -9.79 | 5.04 | 4.40 | 16.94 | -6.72 | 2.75 | 25.95 | 25.18 | 26.62 | -6.35 | -0.00 | 26.14 | 25.26 | 31.25 | -7.62 |
Eisosomes | -0.35 | -4.04 | -9.12 | -10.84 | 0.48 | -1.37 | -9.26 | -5.50 | -4.41 | 3.91 | -0.90 | -9.97 | -8.85 | -8.28 | 3.33 |
Endoplasmic Reticulum | 0.85 | 1.63 | 0.84 | -0.18 | -6.60 | -1.67 | -3.46 | 0.39 | 2.15 | 4.19 | -1.00 | -2.17 | 0.14 | 1.39 | 3.06 |
Endosome | -2.91 | 5.91 | 5.41 | 9.64 | -5.37 | 1.56 | 16.67 | 14.84 | 15.49 | -4.30 | 0.02 | 17.57 | 16.04 | 18.51 | -6.30 |
Golgi | 1.66 | 1.92 | 1.66 | 0.04 | -2.41 | -3.72 | 9.23 | 2.63 | 5.14 | -3.45 | -1.26 | 5.27 | 2.24 | 4.26 | -4.46 |
Lipid Particles | -5.09 | -3.23 | -3.63 | 2.75 | 0.86 | 2.47 | 8.44 | 4.60 | 3.01 | -2.70 | 1.63 | 7.88 | 5.17 | 4.44 | -2.83 |
Mitochondria | -2.08 | 1.36 | -0.59 | 1.91 | -3.55 | -1.85 | 4.44 | 3.69 | 5.44 | -0.47 | -2.35 | 4.67 | 2.98 | 5.13 | -1.74 |
Mitotic Spindle | -0.21 | 1.18 | -0.37 | -0.04 | -2.39 | -1.09 | 0.96 | -1.18 | 0.20 | -2.83 | -1.27 | 1.26 | -2.29 | -0.59 | -3.85 |
None | -2.25 | 1.44 | -1.59 | 2.08 | -2.25 | -0.62 | 2.74 | -0.19 | 0.23 | -1.21 | -1.73 | 2.82 | 0.39 | 1.76 | -1.26 |
Nuclear Periphery | 0.04 | -7.19 | 0.81 | 0.40 | 8.42 | -0.70 | 4.33 | 1.68 | 1.23 | -1.80 | -0.80 | -4.40 | -0.20 | 0.69 | 3.74 |
Nuclear Periphery Foci | 0.97 | 3.64 | 2.30 | 1.27 | -3.94 | -1.05 | -0.88 | -1.31 | -1.05 | -1.20 | -1.38 | 1.07 | -1.75 | -1.11 | -2.00 |
Nucleolus | -3.79 | 2.32 | 2.77 | 4.48 | 1.48 | 0.94 | 6.58 | 6.70 | 6.37 | 2.60 | -1.26 | 6.73 | 6.70 | 7.35 | 0.02 |
Nucleus | -1.05 | 2.45 | 1.68 | 7.08 | -5.43 | -1.10 | 10.02 | 15.23 | 16.72 | 4.64 | -1.13 | 10.41 | 14.60 | 18.09 | 3.94 |
Peroxisomes | -6.54 | 3.81 | 1.82 | 7.30 | -3.40 | -4.00 | 8.69 | 9.51 | 9.77 | 0.75 | -5.60 | 9.04 | 9.38 | 11.80 | 0.13 |
Vacuole | 13.77 | 14.77 | 18.62 | 10.72 | 9.48 | 3.09 | 24.37 | 20.10 | 20.13 | -8.32 | 6.81 | 25.13 | 22.94 | 22.31 | -5.76 |
Vacuole Periphery | 2.13 | 1.96 | 2.25 | 1.59 | 4.06 | -0.47 | 8.90 | 5.64 | 5.13 | -3.76 | 1.66 | 4.21 | 3.33 | 3.83 | -2.58 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|