Standard name | |
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Human Ortholog | |
Description | Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 6 | 3 | 0 | 1 | 1 | 0 | 2 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 8 | 7 | – | – | – | – | – | – | – | – | – | – | – | – | 24 | 26 | 12 | 1 | 0 | 3 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 7 | 3 | 4 | 0 | 0 | 3 | 0 | 0 | 0 |
Endosome | 3 | 12 | 18 | – | – | – | – | – | – | – | – | – | – | – | – | 63 | 46 | 24 | 33 | 44 | 48 | 11 | 51 | 47 |
Golgi | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 4 | 17 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 8 | 16 | 6 | 1 | 2 | 0 | 0 | 3 | 4 |
Nucleus | 1 | 2 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 3 | 1 | 1 | 2 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
Nucleolus | 8 | 9 | 6 | – | – | – | – | – | – | – | – | – | – | – | – | 11 | 13 | 3 | 0 | 1 | 0 | 3 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 7 | 20 | – | – | – | – | – | – | – | – | – | – | – | – | 55 | 26 | 18 | 6 | 13 | 11 | 1 | 4 | 1 |
Vac/Vac Membrane | 5 | 11 | 8 | – | – | – | – | – | – | – | – | – | – | – | – | 15 | 8 | 7 | 6 | 14 | 7 | 1 | 9 | 8 |
Unique Cell Count | 19 | 55 | 43 | 128 | 92 | 53 | 58 | 92 | 84 | 26 | 75 | 68 | ||||||||||||
Labelled Cell Count | 27 | 67 | 62 | 199 | 150 | 77 | 58 | 92 | 84 | 26 | 75 | 68 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.9 | 6.6 | – | – | – | – | – | – | – | – | – | – | – | – | 6.3 | 7.0 | 7.3 | – | 6.1 | 7.6 |
Std Deviation (1e-4) | 0.9 | 1.2 | 2.3 | – | – | – | – | – | – | – | – | – | – | – | – | 1.5 | 1.7 | 1.6 | – | 0.8 | 2.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | -0.05 | 0.08 | 0.14 | – | -0.12 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0.4 | 1.5 | 0.8 |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0.8 | 0.9 | 0.3 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 2.5 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | -0.4 | -2.1 | -1.3 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -1.1 | -1.7 | -0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.0158 | 7.5067 | 12.8116 | 15.1828 | 7.349 | 8.3926 | 10.9106 | 17.2892 | 15.8436 | 14.0406 |
Translational Efficiency | 0.4053 | 0.6007 | 0.4145 | 0.4503 | 0.526 | 0.7333 | 0.5832 | 0.29 | 0.4386 | 0.4668 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1162 | 759 | 1783 | 190 | 879 | 362 | 199 | 1458 | 2041 | 1121 | 1982 | 1648 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 798.10 | 709.71 | 833.29 | 1055.22 | 652.83 | 764.24 | 900.14 | 863.33 | 735.54 | 727.32 | 840.00 | 885.45 |
Standard Deviation | 132.46 | 117.24 | 111.89 | 168.84 | 107.48 | 108.94 | 108.55 | 143.78 | 141.91 | 117.43 | 113.35 | 159.16 |
Intensity Change Log 2 | — | -0.169340 | 0.062249 | 0.402902 | — | 0.227318 | 0.463442 | 0.403205 | — | 0.022710 | 0.256652 | 0.403038 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000534 | 0.000270 | 0.000255 | 0.000284 | 0.000241 | 0.000473 | 0.000315 | 0.000378 | 0.000408 | 0.000335 | 0.000261 | 0.000367 |
Bud Neck | 0.035115 | 0.053057 | 0.091661 | 0.037810 | 0.060280 | 0.048878 | 0.052886 | 0.038557 | 0.045953 | 0.051708 | 0.087768 | 0.038471 |
Bud Site | 0.036961 | 0.076673 | 0.062033 | 0.089414 | 0.060054 | 0.057914 | 0.090814 | 0.054402 | 0.046907 | 0.070615 | 0.064923 | 0.058439 |
Cell Periphery | 0.000304 | 0.000420 | 0.000217 | 0.000134 | 0.000504 | 0.000575 | 0.000093 | 0.000391 | 0.000390 | 0.000470 | 0.000204 | 0.000362 |
Cytoplasm | 0.042391 | 0.065485 | 0.024469 | 0.025426 | 0.078969 | 0.043579 | 0.024806 | 0.025668 | 0.058144 | 0.058411 | 0.024503 | 0.025640 |
Cytoplasmic Foci | 0.076415 | 0.155525 | 0.081543 | 0.085303 | 0.201864* | 0.115951 | 0.088943 | 0.068347 | 0.130442 | 0.142746 | 0.082286 | 0.070302 |
Eisosomes | 0.000042 | 0.000060 | 0.000081 | 0.000023 | 0.000123 | 0.000074 | 0.000039 | 0.000052 | 0.000077 | 0.000065 | 0.000077 | 0.000049 |
Endoplasmic Reticulum | 0.002204 | 0.001179 | 0.002030 | 0.000394 | 0.002481 | 0.001014 | 0.000911 | 0.001042 | 0.002323 | 0.001126 | 0.001917 | 0.000968 |
Endosome | 0.362657* | 0.324868* | 0.343094* | 0.286728* | 0.286524* | 0.406451* | 0.372133* | 0.422061* | 0.329869* | 0.351213* | 0.346009* | 0.406458* |
Golgi | 0.021400 | 0.062438 | 0.020251 | 0.026464 | 0.078315 | 0.042263 | 0.023340 | 0.036164 | 0.045911 | 0.055923 | 0.020561 | 0.035045 |
Lipid Particles | 0.003050 | 0.004517 | 0.003558 | 0.001534 | 0.011726 | 0.008931 | 0.001201 | 0.002836 | 0.006787 | 0.005942 | 0.003321 | 0.002686 |
Mitochondria | 0.012354 | 0.035182 | 0.008414 | 0.001600 | 0.059154 | 0.031360 | 0.005396 | 0.016605 | 0.032509 | 0.033948 | 0.008111 | 0.014875 |
Mitotic Spindle | 0.040486 | 0.027217 | 0.089367 | 0.042177 | 0.011255 | 0.016238 | 0.104964* | 0.040383 | 0.027897 | 0.023671 | 0.090933 | 0.040590 |
None | 0.000532 | 0.003668 | 0.001531 | 0.000117 | 0.003607 | 0.001138 | 0.000646 | 0.002462 | 0.001856 | 0.002851 | 0.001443 | 0.002192 |
Nuclear Periphery | 0.001076 | 0.000312 | 0.001263 | 0.000526 | 0.000240 | 0.000855 | 0.000623 | 0.000592 | 0.000716 | 0.000487 | 0.001198 | 0.000584 |
Nuclear Periphery Foci | 0.002415 | 0.003268 | 0.007748 | 0.002457 | 0.002031 | 0.001478 | 0.005633 | 0.003251 | 0.002250 | 0.002690 | 0.007536 | 0.003160 |
Nucleolus | 0.009994 | 0.007674 | 0.027718 | 0.002940 | 0.006460 | 0.005420 | 0.011109 | 0.007959 | 0.008472 | 0.006946 | 0.026050 | 0.007381 |
Nucleus | 0.018097 | 0.010090 | 0.048944 | 0.002246 | 0.011389 | 0.008859 | 0.011590 | 0.013230 | 0.015208 | 0.009692 | 0.045194 | 0.011964 |
Peroxisomes | 0.020996 | 0.027299 | 0.042136 | 0.023483 | 0.023126 | 0.032652 | 0.032864 | 0.019820 | 0.021913 | 0.029027 | 0.041205 | 0.020243 |
Vacuole | 0.282371* | 0.103110 | 0.128482 | 0.331586* | 0.068740 | 0.134470 | 0.151972 | 0.212906* | 0.190366 | 0.113237 | 0.130841 | 0.226589* |
Vacuole Periphery | 0.030605 | 0.037687 | 0.015206 | 0.039354 | 0.032918 | 0.041427 | 0.019720 | 0.032893 | 0.031601 | 0.038895 | 0.015659 | 0.033637 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.45 | 2.48 | 1.76 | -0.50 | -0.44 | -1.46 | -1.32 | -1.39 | 0.58 | 0.75 | 0.97 | 1.51 | 0.56 | -0.34 | -0.76 |
Bud Neck | -3.65 | -13.72 | -0.84 | 1.99 | 8.48 | 1.72 | 1.02 | 4.36 | 1.82 | 1.93 | -1.44 | -10.76 | 2.18 | 3.18 | 12.69 |
Bud Site | -7.01 | -7.83 | -5.67 | -1.41 | -3.00 | 0.26 | -2.58 | 0.97 | 0.40 | 3.05 | -5.16 | -5.77 | -3.48 | 2.27 | 1.66 |
Cell Periphery | -0.91 | 0.54 | 0.97 | 3.88 | 1.64 | -0.29 | 6.78 | 0.92 | 0.73 | -2.48 | -0.76 | 2.16 | 0.17 | 0.83 | -1.50 |
Cytoplasm | -3.00 | 5.49 | 2.29 | 4.30 | -0.87 | 4.75 | 5.91 | 9.08 | 2.68 | 0.08 | 0.36 | 9.88 | 9.11 | 6.70 | -0.68 |
Cytoplasmic Foci | -12.19 | -1.57 | -0.81 | 7.54 | -0.07 | 8.96 | 10.86 | 19.25 | 6.40 | 2.08 | -2.21 | 11.09 | 14.04 | 13.88 | 3.32 |
Eisosomes | -3.82 | -9.82 | 6.88 | 9.46 | 16.16 | 4.20 | 7.02 | 7.04 | 2.89 | -0.65 | 2.50 | -0.23 | 5.41 | 3.52 | 6.95 |
Endoplasmic Reticulum | 2.12 | 0.18 | 3.71 | 4.91 | 12.53 | 2.04 | 1.49 | 2.02 | -0.20 | 0.89 | 2.89 | 0.59 | 3.28 | 1.08 | 7.60 |
Endosome | 1.44 | 1.48 | 4.14 | 3.02 | 3.43 | -6.85 | -2.92 | -10.67 | -0.31 | -3.12 | -3.07 | -1.19 | -7.99 | -3.98 | -6.87 |
Golgi | -10.84 | 0.30 | -1.02 | 7.73 | -1.25 | 6.42 | 12.29 | 10.18 | 1.65 | -4.43 | -3.12 | 11.78 | 4.47 | 6.97 | -8.01 |
Lipid Particles | -1.36 | -0.56 | 2.00 | 2.75 | 2.36 | 0.93 | 5.37 | 4.90 | 2.33 | -1.65 | 0.73 | 3.51 | 4.71 | 3.03 | 1.16 |
Mitochondria | -6.37 | 2.00 | 6.30 | 10.15 | 9.04 | 4.72 | 12.23 | 9.87 | 3.42 | -5.33 | -0.53 | 11.20 | 7.35 | 6.72 | -4.81 |
Mitotic Spindle | 3.48 | -8.59 | 0.02 | -1.73 | 4.80 | -1.29 | -6.57 | -8.02 | -5.40 | 4.59 | 1.94 | -13.51 | -3.20 | -4.67 | 10.03 |
None | -3.44 | -3.93 | 4.71 | 3.90 | 5.79 | 2.21 | 2.51 | 1.01 | -1.59 | -2.05 | -1.18 | 0.88 | -0.28 | 0.81 | -1.04 |
Nuclear Periphery | 6.12 | -0.82 | 2.79 | -2.56 | 3.95 | -1.30 | -4.53 | -4.35 | 0.60 | 0.75 | 1.30 | -4.04 | 1.46 | -0.55 | 5.56 |
Nuclear Periphery Foci | -0.07 | -4.93 | 0.80 | 0.75 | 5.81 | 0.73 | -3.15 | -1.28 | -2.34 | 2.45 | 0.03 | -5.98 | -0.54 | -0.51 | 5.55 |
Nucleolus | 1.58 | -8.29 | 5.08 | 2.40 | 13.96 | 0.39 | -2.13 | -0.97 | -1.17 | 1.75 | 1.34 | -10.08 | 1.37 | -0.31 | 11.47 |
Nucleus | 3.87 | -7.68 | 7.31 | 4.15 | 14.05 | 0.71 | -1.13 | -0.85 | -1.71 | 0.70 | 3.38 | -9.14 | 1.93 | -1.37 | 10.53 |
Peroxisomes | -2.49 | -8.41 | -0.29 | 1.31 | 4.68 | -2.12 | -2.04 | 1.48 | 3.04 | 3.02 | -3.15 | -8.66 | 1.05 | 3.85 | 9.26 |
Vacuole | 19.11 | 17.59 | -2.69 | -12.56 | -11.35 | -6.37 | -6.38 | -20.60 | -7.62 | -4.58 | 11.24 | 9.27 | -4.73 | -15.89 | -14.34 |
Vacuole Periphery | -2.47 | 7.04 | -1.31 | 0.18 | -3.94 | -1.97 | 3.73 | 0.27 | 2.29 | -3.87 | -2.98 | 9.45 | -0.94 | 2.19 | -9.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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