Standard name
Human Ortholog
Description Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0.08 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.15 0.16 0.19 0.28 0.23 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0.16 0.22 0.42 0.49 0.5 0.45 0.58 0.49 0.58 0.46 0.69 0.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.31 0 0.06 0.17 0.11 0 0 0 0 0 0.06
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.16 0.14 0.09 0.14 0.06 0 0 0 0.14 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.21 0.13 0.47 0.43 0.28 0.34 0.11 0.15 0.13 0.06 0.05 0
Vac/Vac Membrane 0.26 0.2 0.19 0.12 0.09 0.13 0.11 0.15 0.1 0.08 0.13 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 0 0 0 3 0 0 0 0
Bud 0 1 1 6 3 0 1 1 0 2 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 2 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 8 7 24 26 12 1 0 3 1 0 0
Endoplasmic Reticulum 0 0 0 7 3 4 0 0 3 0 0 0
Endosome 3 12 18 63 46 24 33 44 48 11 51 47
Golgi 0 0 0 0 3 1 1 2 1 0 0 0
Mitochondria 4 17 1 8 16 6 1 2 0 0 3 4
Nucleus 1 2 0 1 3 1 1 2 1 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 2 0 1 0
Nucleolus 8 9 6 11 13 3 0 1 0 3 1 0
Peroxisomes 0 0 1 5 2 1 0 0 0 0 0 0
SpindlePole 4 7 20 55 26 18 6 13 11 1 4 1
Vac/Vac Membrane 5 11 8 15 8 7 6 14 7 1 9 8
Unique Cell Count 19 55 43 128 92 53 58 92 84 26 75 68
Labelled Cell Count 27 67 62 199 150 77 58 92 84 26 75 68


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.9 6.6 6.3 7.0 7.3 6.1 7.6
Std Deviation (1e-4) 0.9 1.2 2.3 1.5 1.7 1.6 0.8 2.0
Intensity Change (Log2) -0.05 0.08 0.14 -0.12 0.21

WT302468WT302468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cytoplasm 0.4 1.5 0.8
Endoplasmic Reticulum 0 0 0
Endosome 0.8 0.9 0.3
Golgi 0 0 0
Mitochondria 0 2.5 0
Nucleus 0 0 0
Nuclear Periphery 0 0 0
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole -0.4 -2.1 -1.3
Vacuole -1.1 -1.7 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0158 7.5067 12.8116 15.1828 7.349 8.3926 10.9106 17.2892 15.8436 14.0406
Translational Efficiency 0.4053 0.6007 0.4145 0.4503 0.526 0.7333 0.5832 0.29 0.4386 0.4668

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1162 759 1783 190 879 362 199 1458 2041 1121 1982 1648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 798.10 709.71 833.29 1055.22 652.83 764.24 900.14 863.33 735.54 727.32 840.00 885.45
Standard Deviation 132.46 117.24 111.89 168.84 107.48 108.94 108.55 143.78 141.91 117.43 113.35 159.16
Intensity Change Log 2 -0.169340 0.062249 0.402902 0.227318 0.463442 0.403205 0.022710 0.256652 0.403038

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000534 0.000270 0.000255 0.000284 0.000241 0.000473 0.000315 0.000378 0.000408 0.000335 0.000261 0.000367
Bud Neck 0.035115 0.053057 0.091661 0.037810 0.060280 0.048878 0.052886 0.038557 0.045953 0.051708 0.087768 0.038471
Bud Site 0.036961 0.076673 0.062033 0.089414 0.060054 0.057914 0.090814 0.054402 0.046907 0.070615 0.064923 0.058439
Cell Periphery 0.000304 0.000420 0.000217 0.000134 0.000504 0.000575 0.000093 0.000391 0.000390 0.000470 0.000204 0.000362
Cytoplasm 0.042391 0.065485 0.024469 0.025426 0.078969 0.043579 0.024806 0.025668 0.058144 0.058411 0.024503 0.025640
Cytoplasmic Foci 0.076415 0.155525 0.081543 0.085303 0.201864 0.115951 0.088943 0.068347 0.130442 0.142746 0.082286 0.070302
Eisosomes 0.000042 0.000060 0.000081 0.000023 0.000123 0.000074 0.000039 0.000052 0.000077 0.000065 0.000077 0.000049
Endoplasmic Reticulum 0.002204 0.001179 0.002030 0.000394 0.002481 0.001014 0.000911 0.001042 0.002323 0.001126 0.001917 0.000968
Endosome 0.362657 0.324868 0.343094 0.286728 0.286524 0.406451 0.372133 0.422061 0.329869 0.351213 0.346009 0.406458
Golgi 0.021400 0.062438 0.020251 0.026464 0.078315 0.042263 0.023340 0.036164 0.045911 0.055923 0.020561 0.035045
Lipid Particles 0.003050 0.004517 0.003558 0.001534 0.011726 0.008931 0.001201 0.002836 0.006787 0.005942 0.003321 0.002686
Mitochondria 0.012354 0.035182 0.008414 0.001600 0.059154 0.031360 0.005396 0.016605 0.032509 0.033948 0.008111 0.014875
Mitotic Spindle 0.040486 0.027217 0.089367 0.042177 0.011255 0.016238 0.104964 0.040383 0.027897 0.023671 0.090933 0.040590
None 0.000532 0.003668 0.001531 0.000117 0.003607 0.001138 0.000646 0.002462 0.001856 0.002851 0.001443 0.002192
Nuclear Periphery 0.001076 0.000312 0.001263 0.000526 0.000240 0.000855 0.000623 0.000592 0.000716 0.000487 0.001198 0.000584
Nuclear Periphery Foci 0.002415 0.003268 0.007748 0.002457 0.002031 0.001478 0.005633 0.003251 0.002250 0.002690 0.007536 0.003160
Nucleolus 0.009994 0.007674 0.027718 0.002940 0.006460 0.005420 0.011109 0.007959 0.008472 0.006946 0.026050 0.007381
Nucleus 0.018097 0.010090 0.048944 0.002246 0.011389 0.008859 0.011590 0.013230 0.015208 0.009692 0.045194 0.011964
Peroxisomes 0.020996 0.027299 0.042136 0.023483 0.023126 0.032652 0.032864 0.019820 0.021913 0.029027 0.041205 0.020243
Vacuole 0.282371 0.103110 0.128482 0.331586 0.068740 0.134470 0.151972 0.212906 0.190366 0.113237 0.130841 0.226589
Vacuole Periphery 0.030605 0.037687 0.015206 0.039354 0.032918 0.041427 0.019720 0.032893 0.031601 0.038895 0.015659 0.033637

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.45 2.48 1.76 -0.50 -0.44 -1.46 -1.32 -1.39 0.58 0.75 0.97 1.51 0.56 -0.34 -0.76
Bud Neck -3.65 -13.72 -0.84 1.99 8.48 1.72 1.02 4.36 1.82 1.93 -1.44 -10.76 2.18 3.18 12.69
Bud Site -7.01 -7.83 -5.67 -1.41 -3.00 0.26 -2.58 0.97 0.40 3.05 -5.16 -5.77 -3.48 2.27 1.66
Cell Periphery -0.91 0.54 0.97 3.88 1.64 -0.29 6.78 0.92 0.73 -2.48 -0.76 2.16 0.17 0.83 -1.50
Cytoplasm -3.00 5.49 2.29 4.30 -0.87 4.75 5.91 9.08 2.68 0.08 0.36 9.88 9.11 6.70 -0.68
Cytoplasmic Foci -12.19 -1.57 -0.81 7.54 -0.07 8.96 10.86 19.25 6.40 2.08 -2.21 11.09 14.04 13.88 3.32
Eisosomes -3.82 -9.82 6.88 9.46 16.16 4.20 7.02 7.04 2.89 -0.65 2.50 -0.23 5.41 3.52 6.95
Endoplasmic Reticulum 2.12 0.18 3.71 4.91 12.53 2.04 1.49 2.02 -0.20 0.89 2.89 0.59 3.28 1.08 7.60
Endosome 1.44 1.48 4.14 3.02 3.43 -6.85 -2.92 -10.67 -0.31 -3.12 -3.07 -1.19 -7.99 -3.98 -6.87
Golgi -10.84 0.30 -1.02 7.73 -1.25 6.42 12.29 10.18 1.65 -4.43 -3.12 11.78 4.47 6.97 -8.01
Lipid Particles -1.36 -0.56 2.00 2.75 2.36 0.93 5.37 4.90 2.33 -1.65 0.73 3.51 4.71 3.03 1.16
Mitochondria -6.37 2.00 6.30 10.15 9.04 4.72 12.23 9.87 3.42 -5.33 -0.53 11.20 7.35 6.72 -4.81
Mitotic Spindle 3.48 -8.59 0.02 -1.73 4.80 -1.29 -6.57 -8.02 -5.40 4.59 1.94 -13.51 -3.20 -4.67 10.03
None -3.44 -3.93 4.71 3.90 5.79 2.21 2.51 1.01 -1.59 -2.05 -1.18 0.88 -0.28 0.81 -1.04
Nuclear Periphery 6.12 -0.82 2.79 -2.56 3.95 -1.30 -4.53 -4.35 0.60 0.75 1.30 -4.04 1.46 -0.55 5.56
Nuclear Periphery Foci -0.07 -4.93 0.80 0.75 5.81 0.73 -3.15 -1.28 -2.34 2.45 0.03 -5.98 -0.54 -0.51 5.55
Nucleolus 1.58 -8.29 5.08 2.40 13.96 0.39 -2.13 -0.97 -1.17 1.75 1.34 -10.08 1.37 -0.31 11.47
Nucleus 3.87 -7.68 7.31 4.15 14.05 0.71 -1.13 -0.85 -1.71 0.70 3.38 -9.14 1.93 -1.37 10.53
Peroxisomes -2.49 -8.41 -0.29 1.31 4.68 -2.12 -2.04 1.48 3.04 3.02 -3.15 -8.66 1.05 3.85 9.26
Vacuole 19.11 17.59 -2.69 -12.56 -11.35 -6.37 -6.38 -20.60 -7.62 -4.58 11.24 9.27 -4.73 -15.89 -14.34
Vacuole Periphery -2.47 7.04 -1.31 0.18 -3.94 -1.97 3.73 0.27 2.29 -3.87 -2.98 9.45 -0.94 2.19 -9.88
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps34

Vps34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available