Standard name
Human Ortholog
Description Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0.08 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.15 0.16 0.19 0.28 0.23 0 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0.16 0.22 0.42 0.49 0.5 0.45 0.58 0.49 0.58 0.46 0.69 0.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.21 0.31 0 0.06 0.17 0.11 0 0 0 0 0 0.06
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.42 0.16 0.14 0.09 0.14 0.06 0 0 0 0.14 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.21 0.13 0.47 0.43 0.28 0.34 0.11 0.15 0.13 0.06 0.05 0
Vac/Vac Membrane 0.26 0.2 0.19 0.12 0.09 0.13 0.11 0.15 0.1 0.08 0.13 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 2 0 0 0 3 0 0 0 0
Bud 0 1 1 6 3 0 1 1 0 2 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 2 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 8 7 24 26 12 1 0 3 1 0 0
Endoplasmic Reticulum 0 0 0 7 3 4 0 0 3 0 0 0
Endosome 3 12 18 63 46 24 33 44 48 11 51 47
Golgi 0 0 0 0 3 1 1 2 1 0 0 0
Mitochondria 4 17 1 8 16 6 1 2 0 0 3 4
Nucleus 1 2 0 1 3 1 1 2 1 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 0 2 0 1 0
Nucleolus 8 9 6 11 13 3 0 1 0 3 1 0
Peroxisomes 0 0 1 5 2 1 0 0 0 0 0 0
SpindlePole 4 7 20 55 26 18 6 13 11 1 4 1
Vac/Vac Membrane 5 11 8 15 8 7 6 14 7 1 9 8
Unique Cell Count 19 55 43 128 92 53 58 92 84 26 75 68
Labelled Cell Count 27 67 62 199 150 77 58 92 84 26 75 68


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.9 6.6 6.3 7.0 7.3 6.1 7.6
Std Deviation (1e-4) 0.9 1.2 2.3 1.5 1.7 1.6 0.8 2.0
Intensity Change (Log2) -0.05 0.08 0.14 -0.12 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cytoplasm 0.4 1.5 0.8
Endoplasmic Reticulum 0 0 0
Endosome 0.8 0.9 0.3
Golgi 0 0 0
Mitochondria 0 2.5 0
Nucleus 0 0 0
Nuclear Periphery 0 0 0
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole -0.4 -2.1 -1.3
Vacuole -1.1 -1.7 -0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0158 7.5067 12.8116 15.1828 7.349 8.3926 10.9106 17.2892 15.8436 14.0406
Translational Efficiency 0.4053 0.6007 0.4145 0.4503 0.526 0.7333 0.5832 0.29 0.4386 0.4668

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1162 759 1783 190 879 362 199 1458 2041 1121 1982 1648

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 798.10 709.71 833.29 1055.22 652.83 764.24 900.14 863.33 735.54 727.32 840.00 885.45
Standard Deviation 132.46 117.24 111.89 168.84 107.48 108.94 108.55 143.78 141.91 117.43 113.35 159.16
Intensity Change Log 2 -0.169340 0.062249 0.402902 0.227318 0.463442 0.403205 0.022710 0.256652 0.403038

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000534 0.000270 0.000255 0.000284 0.000241 0.000473 0.000315 0.000378 0.000408 0.000335 0.000261 0.000367
Bud Neck 0.035115 0.053057 0.091661 0.037810 0.060280 0.048878 0.052886 0.038557 0.045953 0.051708 0.087768 0.038471
Bud Site 0.036961 0.076673 0.062033 0.089414 0.060054 0.057914 0.090814 0.054402 0.046907 0.070615 0.064923 0.058439
Cell Periphery 0.000304 0.000420 0.000217 0.000134 0.000504 0.000575 0.000093 0.000391 0.000390 0.000470 0.000204 0.000362
Cytoplasm 0.042391 0.065485 0.024469 0.025426 0.078969 0.043579 0.024806 0.025668 0.058144 0.058411 0.024503 0.025640
Cytoplasmic Foci 0.076415 0.155525 0.081543 0.085303 0.201864* 0.115951 0.088943 0.068347 0.130442 0.142746 0.082286 0.070302
Eisosomes 0.000042 0.000060 0.000081 0.000023 0.000123 0.000074 0.000039 0.000052 0.000077 0.000065 0.000077 0.000049
Endoplasmic Reticulum 0.002204 0.001179 0.002030 0.000394 0.002481 0.001014 0.000911 0.001042 0.002323 0.001126 0.001917 0.000968
Endosome 0.362657* 0.324868* 0.343094* 0.286728* 0.286524* 0.406451* 0.372133* 0.422061* 0.329869* 0.351213* 0.346009* 0.406458*
Golgi 0.021400 0.062438 0.020251 0.026464 0.078315 0.042263 0.023340 0.036164 0.045911 0.055923 0.020561 0.035045
Lipid Particles 0.003050 0.004517 0.003558 0.001534 0.011726 0.008931 0.001201 0.002836 0.006787 0.005942 0.003321 0.002686
Mitochondria 0.012354 0.035182 0.008414 0.001600 0.059154 0.031360 0.005396 0.016605 0.032509 0.033948 0.008111 0.014875
Mitotic Spindle 0.040486 0.027217 0.089367 0.042177 0.011255 0.016238 0.104964* 0.040383 0.027897 0.023671 0.090933 0.040590
None 0.000532 0.003668 0.001531 0.000117 0.003607 0.001138 0.000646 0.002462 0.001856 0.002851 0.001443 0.002192
Nuclear Periphery 0.001076 0.000312 0.001263 0.000526 0.000240 0.000855 0.000623 0.000592 0.000716 0.000487 0.001198 0.000584
Nuclear Periphery Foci 0.002415 0.003268 0.007748 0.002457 0.002031 0.001478 0.005633 0.003251 0.002250 0.002690 0.007536 0.003160
Nucleolus 0.009994 0.007674 0.027718 0.002940 0.006460 0.005420 0.011109 0.007959 0.008472 0.006946 0.026050 0.007381
Nucleus 0.018097 0.010090 0.048944 0.002246 0.011389 0.008859 0.011590 0.013230 0.015208 0.009692 0.045194 0.011964
Peroxisomes 0.020996 0.027299 0.042136 0.023483 0.023126 0.032652 0.032864 0.019820 0.021913 0.029027 0.041205 0.020243
Vacuole 0.282371* 0.103110 0.128482 0.331586* 0.068740 0.134470 0.151972 0.212906* 0.190366 0.113237 0.130841 0.226589*
Vacuole Periphery 0.030605 0.037687 0.015206 0.039354 0.032918 0.041427 0.019720 0.032893 0.031601 0.038895 0.015659 0.033637

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.45 2.48 1.76 -0.50 -0.44 -1.46 -1.32 -1.39 0.58 0.75 0.97 1.51 0.56 -0.34 -0.76
Bud Neck -3.65 -13.72 -0.84 1.99 8.48 1.72 1.02 4.36 1.82 1.93 -1.44 -10.76 2.18 3.18 12.69
Bud Site -7.01 -7.83 -5.67 -1.41 -3.00 0.26 -2.58 0.97 0.40 3.05 -5.16 -5.77 -3.48 2.27 1.66
Cell Periphery -0.91 0.54 0.97 3.88 1.64 -0.29 6.78 0.92 0.73 -2.48 -0.76 2.16 0.17 0.83 -1.50
Cytoplasm -3.00 5.49 2.29 4.30 -0.87 4.75 5.91 9.08 2.68 0.08 0.36 9.88 9.11 6.70 -0.68
Cytoplasmic Foci -12.19 -1.57 -0.81 7.54 -0.07 8.96 10.86 19.25 6.40 2.08 -2.21 11.09 14.04 13.88 3.32
Eisosomes -3.82 -9.82 6.88 9.46 16.16 4.20 7.02 7.04 2.89 -0.65 2.50 -0.23 5.41 3.52 6.95
Endoplasmic Reticulum 2.12 0.18 3.71 4.91 12.53 2.04 1.49 2.02 -0.20 0.89 2.89 0.59 3.28 1.08 7.60
Endosome 1.44 1.48 4.14 3.02 3.43 -6.85 -2.92 -10.67 -0.31 -3.12 -3.07 -1.19 -7.99 -3.98 -6.87
Golgi -10.84 0.30 -1.02 7.73 -1.25 6.42 12.29 10.18 1.65 -4.43 -3.12 11.78 4.47 6.97 -8.01
Lipid Particles -1.36 -0.56 2.00 2.75 2.36 0.93 5.37 4.90 2.33 -1.65 0.73 3.51 4.71 3.03 1.16
Mitochondria -6.37 2.00 6.30 10.15 9.04 4.72 12.23 9.87 3.42 -5.33 -0.53 11.20 7.35 6.72 -4.81
Mitotic Spindle 3.48 -8.59 0.02 -1.73 4.80 -1.29 -6.57 -8.02 -5.40 4.59 1.94 -13.51 -3.20 -4.67 10.03
None -3.44 -3.93 4.71 3.90 5.79 2.21 2.51 1.01 -1.59 -2.05 -1.18 0.88 -0.28 0.81 -1.04
Nuclear Periphery 6.12 -0.82 2.79 -2.56 3.95 -1.30 -4.53 -4.35 0.60 0.75 1.30 -4.04 1.46 -0.55 5.56
Nuclear Periphery Foci -0.07 -4.93 0.80 0.75 5.81 0.73 -3.15 -1.28 -2.34 2.45 0.03 -5.98 -0.54 -0.51 5.55
Nucleolus 1.58 -8.29 5.08 2.40 13.96 0.39 -2.13 -0.97 -1.17 1.75 1.34 -10.08 1.37 -0.31 11.47
Nucleus 3.87 -7.68 7.31 4.15 14.05 0.71 -1.13 -0.85 -1.71 0.70 3.38 -9.14 1.93 -1.37 10.53
Peroxisomes -2.49 -8.41 -0.29 1.31 4.68 -2.12 -2.04 1.48 3.04 3.02 -3.15 -8.66 1.05 3.85 9.26
Vacuole 19.11 17.59 -2.69 -12.56 -11.35 -6.37 -6.38 -20.60 -7.62 -4.58 11.24 9.27 -4.73 -15.89 -14.34
Vacuole Periphery -2.47 7.04 -1.31 0.18 -3.94 -1.97 3.73 0.27 2.29 -3.87 -2.98 9.45 -0.94 2.19 -9.88
Standard name
Human Ortholog
Description Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Vps34

Vps34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available