| Standard name | |
|---|---|
| Human Ortholog | |
| Description | E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 5 | 1 | 1 | 2 |
| Bud | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | – | 4 | 6 | 0 | 0 | 4 | – | – | – | 2 | 0 | 1 | 1 | 3 | 9 |
| Bud Neck | 0 | 0 | 3 | 2 | 0 | 0 | 3 | 2 | 0 | – | 2 | 2 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 2 | 4 | 3 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | – | 0 | 1 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
| Cytoplasm | 134 | 147 | 170 | 102 | 208 | 236 | 308 | 320 | 195 | – | 111 | 167 | 94 | 193 | 291 | – | – | – | 102 | 130 | 92 | 109 | 169 | 181 |
| Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 2 | 1 | 1 | – | – | – | 2 | 1 | 2 | 1 | 0 | 4 |
| Endosome | 10 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 7 | 10 | 0 | – | – | – | 13 | 4 | 3 | 3 | 1 | 4 |
| Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 11 | 33 | 18 | 55 | 58 | 69 | 122 | 129 | 90 | – | 73 | 130 | 16 | 9 | 23 | – | – | – | 32 | 23 | 36 | 5 | 10 | 17 |
| Nucleus | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 0 | – | 1 | 1 | 11 | 12 | 1 | – | – | – | 6 | 5 | 2 | 6 | 7 | 6 |
| Nuclear Periphery | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 1 |
| Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | – | 4 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 6 | 6 | – | 2 | 6 | 0 | 0 | 0 | – | – | – | 9 | 3 | 2 | 6 | 12 | 14 |
| Vac/Vac Membrane | 25 | 11 | 0 | 3 | 11 | 5 | 2 | 3 | 2 | – | 0 | 2 | 47 | 28 | 3 | – | – | – | 28 | 21 | 10 | 23 | 29 | 39 |
| Unique Cell Count | 168 | 177 | 181 | 140 | 255 | 270 | 351 | 397 | 246 | 154 | 257 | 162 | 239 | 306 | 215 | 204 | 169 | 169 | 249 | 297 | ||||
| Labelled Cell Count | 183 | 195 | 195 | 167 | 283 | 314 | 440 | 468 | 302 | 197 | 317 | 177 | 254 | 324 | 215 | 204 | 169 | 169 | 249 | 297 | ||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.4 | 3.5 | 3.6 | 3.2 | 3.3 | 3.4 | 2.7 | 2.9 | 2.6 | – | 2.8 | 2.2 | 4.1 | 4.4 | 3.7 | – | – | – | 4.4 | 4.1 | 4.5 |
| Std Deviation (1e-4) | 0.9 | 1.2 | 2.2 | 1.5 | 1.2 | 2.4 | 1.3 | 1.5 | 1.3 | – | 1.5 | 1.4 | 1.3 | 1.4 | 1.2 | – | – | – | 1.5 | 1.6 | 1.5 |
| Intensity Change (Log2) | – | – | – | -0.19 | -0.13 | -0.09 | -0.39 | -0.31 | -0.48 | – | -0.39 | -0.69 | 0.2 | 0.27 | 0.05 | – | – | – | 0.28 | 0.2 | 0.32 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.0569 | 0.0088 | 0.0397 | 0.0604 | 0.0729 | 0.0031 | 0.0148 | 0.0003 | 0.0072 | 0.002 | 0.0038 | 0.0009 | – | – | – | – | – | – |
| Bud | 0.0013 | 0.0005 | 0.0006 | 0.0019 | 0.0027 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | – | – | – | – | – | – |
| Bud Neck | 0.0062 | 0.0043 | 0.0023 | 0.0263 | 0.0042 | 0.0059 | 0.001 | 0.0004 | 0.0015 | 0.0014 | 0.0009 | 0.0036 | – | – | – | – | – | – |
| Bud Periphery | 0.0045 | 0.0007 | 0.0009 | 0.0113 | 0.005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | – | – | – | – | – | – |
| Bud Site | 0.0046 | 0.0167 | 0.0112 | 0.03 | 0.0222 | 0.0004 | 0.0011 | 0.0014 | 0.0046 | 0.0005 | 0.0004 | 0.0002 | – | – | – | – | – | – |
| Cell Periphery | 0.001 | 0.0008 | 0.0003 | 0.0018 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
| Cytoplasm | 0.2149 | 0.2736 | 0.2543 | 0.2617 | 0.2624 | 0.3249 | 0.2167 | 0.3306 | 0.308 | 0.4362 | 0.2977 | 0.3196 | – | – | – | – | – | – |
| Cytoplasmic Foci | 0.0303 | 0.0096 | 0.016 | 0.012 | 0.0082 | 0.0162 | 0.0768 | 0.0163 | 0.0153 | 0.0147 | 0.0123 | 0.0144 | – | – | – | – | – | – |
| Eisosomes | 0.0018 | 0.0003 | 0.0005 | 0.0007 | 0.0022 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – |
| Endoplasmic Reticulum | 0.0133 | 0.0058 | 0.005 | 0.015 | 0.0054 | 0.0041 | 0.0072 | 0.0036 | 0.0022 | 0.0044 | 0.0033 | 0.004 | – | – | – | – | – | – |
| Endosome | 0.0307 | 0.0018 | 0.0084 | 0.0193 | 0.0133 | 0.008 | 0.0446 | 0.0024 | 0.0072 | 0.0309 | 0.0198 | 0.0116 | – | – | – | – | – | – |
| Golgi | 0.0064 | 0.0007 | 0.0062 | 0.0074 | 0.0121 | 0.0009 | 0.0132 | 0.0001 | 0.003 | 0.0111 | 0.0193 | 0.0036 | – | – | – | – | – | – |
| Lipid Particles | 0.0198 | 0.0024 | 0.0072 | 0.0077 | 0.0181 | 0.006 | 0.0688 | 0.0002 | 0.0017 | 0.0005 | 0.0046 | 0.0121 | – | – | – | – | – | – |
| Mitochondria | 0.0111 | 0.0027 | 0.0118 | 0.0655 | 0.0235 | 0.0083 | 0.0087 | 0.001 | 0.0008 | 0.002 | 0.0689 | 0.0009 | – | – | – | – | – | – |
| None | 0.4511 | 0.5886 | 0.4958 | 0.3142 | 0.5159 | 0.3923 | 0.4082 | 0.4407 | 0.4277 | 0.3428 | 0.398 | 0.3739 | – | – | – | – | – | – |
| Nuclear Periphery | 0.0285 | 0.0027 | 0.0074 | 0.017 | 0.0028 | 0.0086 | 0.0227 | 0.0075 | 0.0047 | 0.0113 | 0.0064 | 0.0064 | – | – | – | – | – | – |
| Nucleolus | 0.0035 | 0.0013 | 0.0022 | 0.0025 | 0.0008 | 0.0025 | 0.0012 | 0.0015 | 0.0008 | 0.0009 | 0.0008 | 0.0016 | – | – | – | – | – | – |
| Nucleus | 0.0529 | 0.0641 | 0.0886 | 0.1128 | 0.0106 | 0.1866 | 0.0713 | 0.1693 | 0.1939 | 0.1249 | 0.1324 | 0.2108 | – | – | – | – | – | – |
| Peroxisomes | 0.0109 | 0.0038 | 0.0049 | 0.0095 | 0.0046 | 0.002 | 0.0192 | 0.0002 | 0.0093 | 0.0009 | 0.0036 | 0.0015 | – | – | – | – | – | – |
| Punctate Nuclear | 0.033 | 0.0092 | 0.0339 | 0.008 | 0.0025 | 0.025 | 0.0205 | 0.0219 | 0.0103 | 0.0117 | 0.0226 | 0.0325 | – | – | – | – | – | – |
| Vacuole | 0.0106 | 0.0016 | 0.0024 | 0.0107 | 0.0077 | 0.0037 | 0.0025 | 0.0021 | 0.0013 | 0.0023 | 0.0035 | 0.0017 | – | – | – | – | – | – |
| Vacuole Periphery | 0.0066 | 0.0001 | 0.0006 | 0.0041 | 0.0015 | 0.0008 | 0.0011 | 0.0002 | 0.0002 | 0.0009 | 0.0011 | 0.0003 | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 16.4176 | 9.2915 | 17.8635 | 24.3743 | 13.4761 | 15.11 | 23.6467 | 27.1643 | 25.9895 | 20.2198 |
| Translational Efficiency | 0.3052 | 0.5893 | 0.3764 | 0.3096 | 0.3064 | 0.3554 | 0.241 | 0.2509 | 0.2791 | 0.2845 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
Micrographs
Cell Count
| R1 | R2 | R1 & R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
| 189 | 653 | 160 | 1262 | 1791 | 1103 | 194 | 1135 | 1980 | 1756 | 354 | 2397 |
Protein Abundance
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Mean | 591.71 | 695.36 | 1047.57 | 784.90 | 568.29 | 704.07 | 909.53 | 799.33 | 570.53 | 700.83 | 971.92 | 791.73 |
| Standard Deviation | 76.38 | 113.58 | 124.88 | 104.35 | 72.88 | 91.69 | 96.47 | 113.82 | 73.54 | 100.48 | 129.88 | 109.18 |
| Intensity Change Log 2 | — | 0.232870 | 0.824084 | 0.407619 | — | 0.309092 | 0.678494 | 0.492164 | — | 0.270715 | 0.754593 | 0.449657 |
Localization Score (DeepLoc)
| R1 | R2 | R1 & R2 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
| Actin | 0.000027 | 0.000349 | 0.001258 | 0.000454 | 0.000106 | 0.000266 | 0.000445 | 0.000910 | 0.000099 | 0.000297 | 0.000812 | 0.000670 |
| Bud Neck | 0.010020 | 0.021717 | 0.001770 | 0.007381 | 0.013135 | 0.022599 | 0.002523 | 0.007860 | 0.012837 | 0.022271 | 0.002183 | 0.007608 |
| Bud Site | 0.001001 | 0.016031 | 0.003308 | 0.030142 | 0.002080 | 0.009461 | 0.001255 | 0.009714 | 0.001977 | 0.011904 | 0.002183 | 0.020469 |
| Cell Periphery | 0.000271 | 0.002971 | 0.000287 | 0.000213 | 0.000131 | 0.000212 | 0.000163 | 0.000169 | 0.000145 | 0.001238 | 0.000219 | 0.000192 |
| Cytoplasm | 0.360222* | 0.342612* | 0.186287* | 0.340051* | 0.406539* | 0.352062* | 0.097605 | 0.210761* | 0.402118* | 0.348548* | 0.137687* | 0.278831* |
| Cytoplasmic Foci | 0.113089 | 0.211288* | 0.007555 | 0.016393 | 0.180076* | 0.192304* | 0.006917 | 0.007940 | 0.173681 | 0.199364* | 0.007206 | 0.012390 |
| Eisosomes | 0.000109 | 0.003712 | 0.000093 | 0.000049 | 0.000150 | 0.000131 | 0.000082 | 0.000042 | 0.000146 | 0.001463 | 0.000087 | 0.000046 |
| Endoplasmic Reticulum | 0.000478 | 0.001208 | 0.012853 | 0.002090 | 0.001090 | 0.000955 | 0.008016 | 0.004628 | 0.001032 | 0.001049 | 0.010202 | 0.003292 |
| Endosome | 0.004009 | 0.014470 | 0.003867 | 0.005847 | 0.007959 | 0.014883 | 0.002002 | 0.004711 | 0.007582 | 0.014729 | 0.002845 | 0.005309 |
| Golgi | 0.000370 | 0.002920 | 0.000219 | 0.001682 | 0.001638 | 0.005012 | 0.000062 | 0.005082 | 0.001517 | 0.004234 | 0.000133 | 0.003292 |
| Lipid Particles | 0.014398 | 0.010010 | 0.002598 | 0.001586 | 0.017853 | 0.013310 | 0.002404 | 0.001816 | 0.017523 | 0.012083 | 0.002492 | 0.001695 |
| Mitochondria | 0.001491 | 0.014948 | 0.000398 | 0.004669 | 0.007888 | 0.011940 | 0.000499 | 0.004758 | 0.007277 | 0.013059 | 0.000453 | 0.004711 |
| Mitotic Spindle | 0.000089 | 0.007684 | 0.015848 | 0.020638 | 0.000959 | 0.007970 | 0.001085 | 0.020557 | 0.000876 | 0.007864 | 0.007758 | 0.020600 |
| None | 0.007690 | 0.006989 | 0.002222 | 0.014786 | 0.011876 | 0.004741 | 0.004666 | 0.007165 | 0.011476 | 0.005577 | 0.003561 | 0.011177 |
| Nuclear Periphery | 0.000890 | 0.001024 | 0.001482 | 0.001762 | 0.001376 | 0.001212 | 0.002263 | 0.003131 | 0.001330 | 0.001142 | 0.001910 | 0.002410 |
| Nuclear Periphery Foci | 0.001337 | 0.000581 | 0.006570 | 0.000640 | 0.000730 | 0.000652 | 0.001167 | 0.001763 | 0.000788 | 0.000625 | 0.003609 | 0.001172 |
| Nucleolus | 0.003090 | 0.004099 | 0.000586 | 0.001320 | 0.003286 | 0.003680 | 0.001032 | 0.001196 | 0.003267 | 0.003836 | 0.000830 | 0.001261 |
| Nucleus | 0.467585* | 0.282962* | 0.611212* | 0.488608* | 0.311830* | 0.306066* | 0.812125* | 0.668398* | 0.326697* | 0.297474* | 0.721317* | 0.573740* |
| Peroxisomes | 0.002597 | 0.017572 | 0.000313 | 0.001785 | 0.005716 | 0.013935 | 0.000266 | 0.000450 | 0.005418 | 0.015287 | 0.000287 | 0.001153 |
| Vacuole | 0.010927 | 0.035375 | 0.141048 | 0.057101 | 0.024677 | 0.035859 | 0.055283 | 0.035527 | 0.023365 | 0.035679 | 0.094047 | 0.046886 |
| Vacuole Periphery | 0.000311 | 0.001478 | 0.000227 | 0.002805 | 0.000904 | 0.002750 | 0.000141 | 0.003422 | 0.000847 | 0.002277 | 0.000180 | 0.003097 |
Localization Changes (T-score)
| R1 | R2 | R1 & R2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
| Actin | -7.74 | -11.31 | -5.63 | -0.91 | 7.56 | -4.27 | -7.68 | -3.29 | -2.55 | -0.54 | -6.37 | -12.54 | -4.61 | -2.82 | 3.58 |
| Bud Neck | -2.82 | 2.02 | 0.43 | 6.02 | -8.65 | -4.08 | 9.31 | 3.54 | 6.13 | -6.33 | -5.27 | 10.40 | 4.12 | 8.49 | -10.00 |
| Bud Site | -4.10 | -6.81 | -6.79 | -0.78 | -5.38 | -5.58 | -0.65 | -5.32 | 0.48 | -4.95 | -6.24 | -3.96 | -7.98 | -1.09 | -6.98 |
| Cell Periphery | -1.50 | -0.73 | 1.80 | 1.56 | 5.32 | -3.67 | -3.66 | -2.54 | 2.07 | 1.74 | -1.64 | -6.30 | -2.24 | 1.61 | 5.00 |
| Cytoplasm | 0.82 | 8.10 | 4.07 | 5.30 | -6.96 | 5.42 | 25.59 | 25.43 | 17.51 | -6.76 | 6.20 | 26.25 | 22.19 | 15.09 | -10.17 |
| Cytoplasmic Foci | -7.16 | 9.82 | 9.14 | 23.35 | -4.89 | -1.83 | 34.01 | 37.99 | 30.68 | 0.45 | -4.05 | 37.44 | 37.92 | 38.17 | -2.14 |
| Eisosomes | -1.69 | -0.52 | 3.87 | 1.71 | 5.93 | 1.37 | 1.57 | 7.50 | 10.36 | 5.64 | -1.66 | 1.94 | 7.93 | 1.77 | 7.99 |
| Endoplasmic Reticulum | -3.73 | -11.64 | -14.09 | -10.71 | 9.09 | 0.93 | -10.37 | -15.55 | -16.09 | 4.18 | -0.20 | -15.18 | -18.99 | -19.58 | 9.68 |
| Endosome | -5.42 | -0.79 | -0.03 | 6.30 | 1.25 | -4.70 | 6.59 | 5.82 | 8.48 | -0.51 | -6.05 | 5.45 | 5.52 | 10.42 | 0.44 |
| Golgi | -4.85 | 1.04 | -1.01 | 3.05 | -1.38 | -2.89 | 10.14 | -0.66 | 1.76 | -2.28 | -3.52 | 9.79 | 0.01 | 2.93 | -2.68 |
| Lipid Particles | 0.98 | 2.56 | 2.91 | 7.85 | 4.74 | 2.70 | 11.46 | 12.99 | 12.18 | 3.62 | 3.77 | 11.88 | 13.30 | 14.53 | 5.71 |
| Mitochondria | -5.14 | 3.88 | -2.48 | 4.54 | -4.69 | -2.14 | 7.52 | 3.83 | 5.05 | -4.00 | -3.40 | 7.62 | 4.32 | 6.93 | -6.05 |
| Mitotic Spindle | -3.88 | -1.27 | -7.00 | -3.05 | -1.39 | -3.81 | -0.91 | -5.71 | -2.43 | -3.38 | -5.01 | -1.44 | -8.65 | -3.87 | -3.05 |
| None | 0.42 | 5.15 | -1.19 | -1.72 | -5.96 | 5.94 | 5.47 | 5.19 | -0.90 | -1.20 | 5.42 | 7.69 | 2.95 | -2.38 | -5.16 |
| Nuclear Periphery | -0.64 | -6.26 | -11.64 | -8.94 | -4.57 | 0.99 | -6.04 | -9.83 | -10.78 | -3.98 | 1.26 | -6.38 | -11.72 | -14.13 | -5.41 |
| Nuclear Periphery Foci | 0.65 | -4.65 | 0.32 | -3.12 | 6.40 | 0.13 | -4.17 | -2.60 | -2.38 | 1.08 | 0.64 | -6.81 | -1.94 | -2.85 | 6.23 |
| Nucleolus | -1.34 | 3.39 | 2.14 | 4.61 | -3.87 | -1.14 | 6.65 | 8.52 | 6.18 | -0.18 | -1.76 | 9.59 | 8.08 | 7.50 | -2.36 |
| Nucleus | 7.31 | -1.92 | -2.61 | -17.37 | 0.01 | 0.75 | -22.26 | -33.30 | -30.51 | 3.85 | 3.39 | -18.91 | -30.48 | -32.55 | 3.14 |
| Peroxisomes | -7.39 | 4.48 | 2.37 | 8.26 | -2.04 | -5.86 | 9.86 | 9.79 | 10.54 | -0.53 | -8.20 | 10.25 | 8.74 | 13.14 | -2.09 |
| Vacuole | -4.86 | -11.39 | -14.67 | -11.94 | 6.58 | -4.92 | -6.72 | -12.23 | -9.43 | 2.15 | -6.39 | -12.34 | -19.72 | -15.52 | 6.04 |
| Vacuole Periphery | -5.60 | 0.61 | -2.99 | 0.02 | -3.16 | -2.62 | 6.26 | -1.21 | 1.12 | -2.41 | -3.13 | 5.96 | -1.91 | 1.23 | -3.77 |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci |
| Localization | |
| Cell Percentages | nucleus (19%), cytoplasm (6%), mixed (50%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Irc20
Irc20
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Irc20-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
