Standard name
Human Ortholog
Description E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.83 0.94 0.73 0.82 0.87 0.88 0.81 0.79 0.72 0.65 0.58 0.81 0.95 0.48 0.64 0.55 0.65 0.68 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.19 0.1 0.39 0.23 0.26 0.35 0.32 0.37 0.47 0.51 0.1 0 0.08 0.15 0.12 0.22 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Vac/Vac Membrane 0.15 0.06 0 0 0 0 0 0 0 0 0 0.29 0.12 0 0.13 0.11 0.06 0.14 0.12 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 1 1 2
Bud 0 0 1 1 1 0 2 3 2 4 6 0 0 4 2 0 1 1 3 9
Bud Neck 0 0 3 2 0 0 3 2 0 2 2 0 0 0 0 1 0 2 4 3
Bud Site 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Cell Periphery 0 0 1 0 0 1 1 0 2 0 1 0 0 1 0 0 0 0 0 1
Cytoplasm 134 147 170 102 208 236 308 320 195 111 167 94 193 291 102 130 92 109 169 181
Endoplasmic Reticulum 1 1 0 0 0 1 0 1 0 0 0 2 1 1 2 1 2 1 0 4
Endosome 10 2 0 0 3 0 0 0 0 0 0 7 10 0 13 4 3 3 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Mitochondria 11 33 18 55 58 69 122 129 90 73 130 16 9 23 32 23 36 5 10 17
Nucleus 0 0 1 1 1 0 1 3 0 1 1 11 12 1 6 5 2 6 7 6
Nuclear Periphery 0 1 1 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 1
Nucleolus 0 0 0 1 0 1 0 0 2 4 1 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 1 1 1 6 6 2 6 0 0 0 9 3 2 6 12 14
Vac/Vac Membrane 25 11 0 3 11 5 2 3 2 0 2 47 28 3 28 21 10 23 29 39
Unique Cell Count 168 177 181 140 255 270 351 397 246 154 257 162 239 306 215 204 169 169 249 297
Labelled Cell Count 183 195 195 167 283 314 440 468 302 197 317 177 254 324 215 204 169 169 249 297


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.5 3.6 3.2 3.3 3.4 2.7 2.9 2.6 2.8 2.2 4.1 4.4 3.7 4.4 4.1 4.5
Std Deviation (1e-4) 0.9 1.2 2.2 1.5 1.2 2.4 1.3 1.5 1.3 1.5 1.4 1.3 1.4 1.2 1.5 1.6 1.5
Intensity Change (Log2) -0.19 -0.13 -0.09 -0.39 -0.31 -0.48 -0.39 -0.69 0.2 0.27 0.05 0.28 0.2 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0569 0.0088 0.0397 0.0604 0.0729 0.0031 0.0148 0.0003 0.0072 0.002 0.0038 0.0009
Bud 0.0013 0.0005 0.0006 0.0019 0.0027 0.0002 0.0001 0.0002 0.0002 0.0003 0.0002 0.0002
Bud Neck 0.0062 0.0043 0.0023 0.0263 0.0042 0.0059 0.001 0.0004 0.0015 0.0014 0.0009 0.0036
Bud Periphery 0.0045 0.0007 0.0009 0.0113 0.005 0.0004 0.0001 0.0001 0.0001 0.0002 0.0003 0.0002
Bud Site 0.0046 0.0167 0.0112 0.03 0.0222 0.0004 0.0011 0.0014 0.0046 0.0005 0.0004 0.0002
Cell Periphery 0.001 0.0008 0.0003 0.0018 0.0017 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.2149 0.2736 0.2543 0.2617 0.2624 0.3249 0.2167 0.3306 0.308 0.4362 0.2977 0.3196
Cytoplasmic Foci 0.0303 0.0096 0.016 0.012 0.0082 0.0162 0.0768 0.0163 0.0153 0.0147 0.0123 0.0144
Eisosomes 0.0018 0.0003 0.0005 0.0007 0.0022 0.0001 0.0001 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0133 0.0058 0.005 0.015 0.0054 0.0041 0.0072 0.0036 0.0022 0.0044 0.0033 0.004
Endosome 0.0307 0.0018 0.0084 0.0193 0.0133 0.008 0.0446 0.0024 0.0072 0.0309 0.0198 0.0116
Golgi 0.0064 0.0007 0.0062 0.0074 0.0121 0.0009 0.0132 0.0001 0.003 0.0111 0.0193 0.0036
Lipid Particles 0.0198 0.0024 0.0072 0.0077 0.0181 0.006 0.0688 0.0002 0.0017 0.0005 0.0046 0.0121
Mitochondria 0.0111 0.0027 0.0118 0.0655 0.0235 0.0083 0.0087 0.001 0.0008 0.002 0.0689 0.0009
None 0.4511 0.5886 0.4958 0.3142 0.5159 0.3923 0.4082 0.4407 0.4277 0.3428 0.398 0.3739
Nuclear Periphery 0.0285 0.0027 0.0074 0.017 0.0028 0.0086 0.0227 0.0075 0.0047 0.0113 0.0064 0.0064
Nucleolus 0.0035 0.0013 0.0022 0.0025 0.0008 0.0025 0.0012 0.0015 0.0008 0.0009 0.0008 0.0016
Nucleus 0.0529 0.0641 0.0886 0.1128 0.0106 0.1866 0.0713 0.1693 0.1939 0.1249 0.1324 0.2108
Peroxisomes 0.0109 0.0038 0.0049 0.0095 0.0046 0.002 0.0192 0.0002 0.0093 0.0009 0.0036 0.0015
Punctate Nuclear 0.033 0.0092 0.0339 0.008 0.0025 0.025 0.0205 0.0219 0.0103 0.0117 0.0226 0.0325
Vacuole 0.0106 0.0016 0.0024 0.0107 0.0077 0.0037 0.0025 0.0021 0.0013 0.0023 0.0035 0.0017
Vacuole Periphery 0.0066 0.0001 0.0006 0.0041 0.0015 0.0008 0.0011 0.0002 0.0002 0.0009 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.4176 9.2915 17.8635 24.3743 13.4761 15.11 23.6467 27.1643 25.9895 20.2198
Translational Efficiency 0.3052 0.5893 0.3764 0.3096 0.3064 0.3554 0.241 0.2509 0.2791 0.2845

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
189 653 160 1262 1791 1103 194 1135 1980 1756 354 2397

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.71 695.36 1047.57 784.90 568.29 704.07 909.53 799.33 570.53 700.83 971.92 791.73
Standard Deviation 76.38 113.58 124.88 104.35 72.88 91.69 96.47 113.82 73.54 100.48 129.88 109.18
Intensity Change Log 2 0.232870 0.824084 0.407619 0.309092 0.678494 0.492164 0.270715 0.754593 0.449657

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000027 0.000349 0.001258 0.000454 0.000106 0.000266 0.000445 0.000910 0.000099 0.000297 0.000812 0.000670
Bud Neck 0.010020 0.021717 0.001770 0.007381 0.013135 0.022599 0.002523 0.007860 0.012837 0.022271 0.002183 0.007608
Bud Site 0.001001 0.016031 0.003308 0.030142 0.002080 0.009461 0.001255 0.009714 0.001977 0.011904 0.002183 0.020469
Cell Periphery 0.000271 0.002971 0.000287 0.000213 0.000131 0.000212 0.000163 0.000169 0.000145 0.001238 0.000219 0.000192
Cytoplasm 0.360222* 0.342612* 0.186287* 0.340051* 0.406539* 0.352062* 0.097605 0.210761* 0.402118* 0.348548* 0.137687* 0.278831*
Cytoplasmic Foci 0.113089 0.211288* 0.007555 0.016393 0.180076* 0.192304* 0.006917 0.007940 0.173681 0.199364* 0.007206 0.012390
Eisosomes 0.000109 0.003712 0.000093 0.000049 0.000150 0.000131 0.000082 0.000042 0.000146 0.001463 0.000087 0.000046
Endoplasmic Reticulum 0.000478 0.001208 0.012853 0.002090 0.001090 0.000955 0.008016 0.004628 0.001032 0.001049 0.010202 0.003292
Endosome 0.004009 0.014470 0.003867 0.005847 0.007959 0.014883 0.002002 0.004711 0.007582 0.014729 0.002845 0.005309
Golgi 0.000370 0.002920 0.000219 0.001682 0.001638 0.005012 0.000062 0.005082 0.001517 0.004234 0.000133 0.003292
Lipid Particles 0.014398 0.010010 0.002598 0.001586 0.017853 0.013310 0.002404 0.001816 0.017523 0.012083 0.002492 0.001695
Mitochondria 0.001491 0.014948 0.000398 0.004669 0.007888 0.011940 0.000499 0.004758 0.007277 0.013059 0.000453 0.004711
Mitotic Spindle 0.000089 0.007684 0.015848 0.020638 0.000959 0.007970 0.001085 0.020557 0.000876 0.007864 0.007758 0.020600
None 0.007690 0.006989 0.002222 0.014786 0.011876 0.004741 0.004666 0.007165 0.011476 0.005577 0.003561 0.011177
Nuclear Periphery 0.000890 0.001024 0.001482 0.001762 0.001376 0.001212 0.002263 0.003131 0.001330 0.001142 0.001910 0.002410
Nuclear Periphery Foci 0.001337 0.000581 0.006570 0.000640 0.000730 0.000652 0.001167 0.001763 0.000788 0.000625 0.003609 0.001172
Nucleolus 0.003090 0.004099 0.000586 0.001320 0.003286 0.003680 0.001032 0.001196 0.003267 0.003836 0.000830 0.001261
Nucleus 0.467585* 0.282962* 0.611212* 0.488608* 0.311830* 0.306066* 0.812125* 0.668398* 0.326697* 0.297474* 0.721317* 0.573740*
Peroxisomes 0.002597 0.017572 0.000313 0.001785 0.005716 0.013935 0.000266 0.000450 0.005418 0.015287 0.000287 0.001153
Vacuole 0.010927 0.035375 0.141048 0.057101 0.024677 0.035859 0.055283 0.035527 0.023365 0.035679 0.094047 0.046886
Vacuole Periphery 0.000311 0.001478 0.000227 0.002805 0.000904 0.002750 0.000141 0.003422 0.000847 0.002277 0.000180 0.003097

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.74 -11.31 -5.63 -0.91 7.56 -4.27 -7.68 -3.29 -2.55 -0.54 -6.37 -12.54 -4.61 -2.82 3.58
Bud Neck -2.82 2.02 0.43 6.02 -8.65 -4.08 9.31 3.54 6.13 -6.33 -5.27 10.40 4.12 8.49 -10.00
Bud Site -4.10 -6.81 -6.79 -0.78 -5.38 -5.58 -0.65 -5.32 0.48 -4.95 -6.24 -3.96 -7.98 -1.09 -6.98
Cell Periphery -1.50 -0.73 1.80 1.56 5.32 -3.67 -3.66 -2.54 2.07 1.74 -1.64 -6.30 -2.24 1.61 5.00
Cytoplasm 0.82 8.10 4.07 5.30 -6.96 5.42 25.59 25.43 17.51 -6.76 6.20 26.25 22.19 15.09 -10.17
Cytoplasmic Foci -7.16 9.82 9.14 23.35 -4.89 -1.83 34.01 37.99 30.68 0.45 -4.05 37.44 37.92 38.17 -2.14
Eisosomes -1.69 -0.52 3.87 1.71 5.93 1.37 1.57 7.50 10.36 5.64 -1.66 1.94 7.93 1.77 7.99
Endoplasmic Reticulum -3.73 -11.64 -14.09 -10.71 9.09 0.93 -10.37 -15.55 -16.09 4.18 -0.20 -15.18 -18.99 -19.58 9.68
Endosome -5.42 -0.79 -0.03 6.30 1.25 -4.70 6.59 5.82 8.48 -0.51 -6.05 5.45 5.52 10.42 0.44
Golgi -4.85 1.04 -1.01 3.05 -1.38 -2.89 10.14 -0.66 1.76 -2.28 -3.52 9.79 0.01 2.93 -2.68
Lipid Particles 0.98 2.56 2.91 7.85 4.74 2.70 11.46 12.99 12.18 3.62 3.77 11.88 13.30 14.53 5.71
Mitochondria -5.14 3.88 -2.48 4.54 -4.69 -2.14 7.52 3.83 5.05 -4.00 -3.40 7.62 4.32 6.93 -6.05
Mitotic Spindle -3.88 -1.27 -7.00 -3.05 -1.39 -3.81 -0.91 -5.71 -2.43 -3.38 -5.01 -1.44 -8.65 -3.87 -3.05
None 0.42 5.15 -1.19 -1.72 -5.96 5.94 5.47 5.19 -0.90 -1.20 5.42 7.69 2.95 -2.38 -5.16
Nuclear Periphery -0.64 -6.26 -11.64 -8.94 -4.57 0.99 -6.04 -9.83 -10.78 -3.98 1.26 -6.38 -11.72 -14.13 -5.41
Nuclear Periphery Foci 0.65 -4.65 0.32 -3.12 6.40 0.13 -4.17 -2.60 -2.38 1.08 0.64 -6.81 -1.94 -2.85 6.23
Nucleolus -1.34 3.39 2.14 4.61 -3.87 -1.14 6.65 8.52 6.18 -0.18 -1.76 9.59 8.08 7.50 -2.36
Nucleus 7.31 -1.92 -2.61 -17.37 0.01 0.75 -22.26 -33.30 -30.51 3.85 3.39 -18.91 -30.48 -32.55 3.14
Peroxisomes -7.39 4.48 2.37 8.26 -2.04 -5.86 9.86 9.79 10.54 -0.53 -8.20 10.25 8.74 13.14 -2.09
Vacuole -4.86 -11.39 -14.67 -11.94 6.58 -4.92 -6.72 -12.23 -9.43 2.15 -6.39 -12.34 -19.72 -15.52 6.04
Vacuole Periphery -5.60 0.61 -2.99 0.02 -3.16 -2.62 6.26 -1.21 1.12 -2.41 -3.13 5.96 -1.91 1.23 -3.77
Standard name
Human Ortholog
Description E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent nonhomologous end joining (NHEJ) pathway; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; interacts with Cdc48p and Smt3p; null mutant displays increased levels of spontaneous Rad52p foci
Localization
Cell Percentages nucleus (19%), cytoplasm (6%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Irc20

Irc20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Irc20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available