Standard name
Human Ortholog
Description Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.06 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0.46 0.74 0.2 0.25 0.53 0.19 0.28 – 0.18 0.19 0.41 0.33 – – – 0.72 0.64 0.68 0.08 0.11 0.14 0 0 0
Cytoplasm 0.96 0.89 0.95 0.9 0.95 0.98 0.96 – 0.95 0.9 0.86 0.88 – – – 0.76 0.82 0.83 0.83 0.78 0.62 0.69 0.82 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.07 0 0.09 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.06 0.08 0 0 0 0 0.14 0.08 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 1 1 0 – – – 0 1 0 1 0 2 0 0 0
Bud 6 2 0 0 1 3 0 – 2 3 1 2 – – – 0 0 0 1 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 2 5 4 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 133 129 9 19 43 16 56 – 10 19 30 32 – – – 273 156 165 23 20 11 0 0 0
Cytoplasm 277 155 42 69 77 84 194 – 52 88 63 86 – – – 288 200 203 246 140 49 8 9 33
Endoplasmic Reticulum 7 3 1 2 3 1 4 – 0 3 1 2 – – – 7 6 10 13 7 5 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 2 1 0 0 0 0
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 – – – 4 0 0 1 0 0 0 0 0
Mitochondria 0 1 1 0 0 1 4 – 0 4 3 3 – – – 3 0 2 1 0 0 0 0 0
Nucleus 2 0 0 3 0 3 2 – 1 0 2 2 – – – 24 20 10 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 2 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 – – – 13 2 0 0 1 1 0 0 0
Unique Cell Count 290 175 44 77 81 86 203 55 98 73 98 377 243 244 298 181 79 13 11 39
Labelled Cell Count 427 291 53 94 124 108 260 65 120 101 127 613 385 390 298 181 79 13 11 39


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 12.7 9.6 9.3 11.2 9.1 9.7 – 8.6 8.8 8.7 8.3 – – – 16.5 16.0 15.5 – – –
Std Deviation (1e-4) 1.3 1.7 1.4 2.2 1.6 1.2 1.6 – 1.3 1.7 1.6 1.1 – – – 2.9 3.0 3.2 – – –
Intensity Change (Log2) – – – -0.05 0.22 -0.08 0.01 – -0.16 -0.13 -0.14 -0.21 – – – 0.78 0.74 0.69 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0.5 3.5 -0.3 1.0 – -0.3 -0.1 2.3 1.5 – – – 6.9 5.4 5.9
Cytoplasm -1.1 -0.1 0.7 0 – -0.2 -1.1 -1.6 -1.4 – – – -2.9 -2.2 -2.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9664 8.0871 6.926 6.0162 6.0114 7.2462 6.6186 8.3864 7.5094 6.7553 7.2105 7.9311 8.3322 10.0009 8.9459 8.4255 8.4752 8.9156
Actin 0.0259 0.0027 0.019 0.0037 0.0159 0.004 0.03 0.0022 0.005 0.0031 0.0071 0.0022 0.0389 0.0024 0.0191 0.0095 0.0222 0.0031
Bud 0.0017 0.0082 0.0052 0.0006 0.0014 0.0019 0.0006 0.0004 0.0015 0.0005 0.0115 0.0001 0.0008 0.0001 0.0019 0.0006 0.0019 0.0001
Bud Neck 0.0071 0.0007 0.0022 0.0044 0.0044 0.0101 0.0136 0.001 0.0018 0.0033 0.0023 0.0043 0.0023 0.001 0.0041 0.0029 0.0073 0.0063
Bud Periphery 0.0044 0.0029 0.0037 0.0029 0.0057 0.0089 0.0042 0.0012 0.0032 0.0023 0.0125 0.0011 0.0027 0.0003 0.0056 0.002 0.0024 0.0009
Bud Site 0.0102 0.0251 0.0079 0.0016 0.0043 0.0012 0.0054 0.0136 0.0067 0.0057 0.0072 0.0008 0.0054 0.0066 0.0133 0.0021 0.0035 0.0003
Cell Periphery 0.0885 0.0226 0.0569 0.0923 0.0614 0.0593 0.1299 0.0447 0.0745 0.061 0.08 0.0873 0.1101 0.0457 0.0624 0.0584 0.0385 0.0389
Cytoplasm 0.7019 0.929 0.8692 0.8409 0.7313 0.8683 0.7377 0.9278 0.8818 0.8762 0.8655 0.8717 0.7557 0.9267 0.8591 0.7861 0.854 0.9171
Cytoplasmic Foci 0.0208 0.0006 0.0052 0.0091 0.0296 0.0064 0.0035 0.0005 0.0008 0.0017 0.0003 0.0018 0.0069 0.0007 0.0029 0.0058 0.0068 0.0004
Eisosomes 0.0005 0 0.0001 0.0001 0.0003 0.0001 0.0013 0.0001 0.0001 0.0002 0.0001 0 0.001 0 0.0001 0.0011 0.0001 0
Endoplasmic Reticulum 0.0226 0.0047 0.0043 0.0055 0.0056 0.0064 0.0306 0.0051 0.0084 0.0264 0.0102 0.0095 0.0334 0.0125 0.0131 0.0064 0.0152 0.0179
Endosome 0.031 0.0002 0.0065 0.0044 0.0426 0.0099 0.0033 0.0001 0.0008 0.0008 0.0001 0.0011 0.0053 0.0002 0.002 0.0039 0.0102 0.0021
Golgi 0.0137 0.0002 0.0065 0.0136 0.0187 0.0087 0.0034 0.0003 0.0005 0.0006 0.0002 0.0008 0.0068 0.0004 0.0007 0.0016 0.0176 0.0012
Lipid Particles 0.0123 0.0003 0.0031 0.0033 0.026 0.0063 0.006 0.0005 0.0009 0.0042 0.0005 0.0038 0.0074 0.001 0.0026 0.0066 0.0026 0.0015
Mitochondria 0.0092 0.0001 0.0029 0.0029 0.0259 0.0033 0.0108 0 0.0003 0.0001 0 0.0001 0.0037 0 0.0001 0.0405 0.0097 0.0005
None 0.0101 0.001 0.001 0.0004 0.0016 0.0008 0.0037 0.0009 0.0032 0.0065 0.0004 0.0016 0.0045 0.0003 0.0017 0.0016 0.0003 0.0002
Nuclear Periphery 0.0063 0.0002 0.0002 0.0003 0.005 0.0005 0.0015 0.0002 0.0017 0.0009 0.0003 0.0037 0.0026 0.0003 0.001 0.0222 0.0007 0.0069
Nucleolus 0.0007 0 0.0001 0.0001 0.0003 0.0001 0.0014 0 0.0001 0.0002 0 0.0002 0.0009 0 0.0001 0.0003 0.0001 0
Nucleus 0.0025 0.0004 0.0005 0.0004 0.0008 0.0004 0.0015 0.0003 0.0047 0.0012 0.0003 0.0006 0.0012 0.0002 0.0005 0.0103 0.0015 0.0003
Peroxisomes 0.0172 0 0.0013 0.0029 0.0115 0.0008 0.0038 0 0 0 0 0.0049 0.0008 0 0.0003 0.0306 0.0031 0
Punctate Nuclear 0.0025 0 0.0001 0.0001 0.0016 0.0002 0.001 0 0.0005 0.0004 0.0001 0.0025 0.0023 0 0.0028 0.0008 0.0003 0.0002
Vacuole 0.0084 0.0011 0.0038 0.0106 0.005 0.0023 0.0066 0.0011 0.0032 0.0046 0.0014 0.0018 0.0071 0.0015 0.0064 0.006 0.0016 0.0017
Vacuole Periphery 0.0022 0 0.0005 0.0001 0.0011 0.0002 0.0003 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.001 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 73.4399 63.4983 67.0839 76.0384 63.6088 66.1796 54.0013 67.7533 78.2092 58.8225
Translational Efficiency 1.6298 1.3498 1.2947 1.3524 1.1292 1.6561 1.3984 1.1098 1.1988 1.3224

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2367 2473 124 66 1275 2321 1331 17 3642 4794 1455 83

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 924.76 1073.81 1616.41 1280.85 964.61 1024.05 1383.08 2094.07 938.71 1049.72 1402.97 1447.41
Standard Deviation 107.11 152.42 196.52 192.51 134.59 141.24 189.21 406.17 118.99 149.20 200.71 413.48
Intensity Change Log 2 — 0.215588 0.805642 0.469951 — 0.086268 0.519867 1.118292 — 0.151013 0.666812 0.836948

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001010 0.001681 0.022835 0.010260 0.003017 0.004124 0.006618 0.001103 0.001712 0.002864 0.008000 0.008384
Bud Neck 0.012637 0.008210 0.017600 0.012490 0.011585 0.012364 0.005890 0.021839 0.012269 0.010221 0.006888 0.014405
Bud Site 0.037272 0.054834 0.109434* 0.112918* 0.058975 0.066409 0.056714 0.037995 0.044870 0.060438 0.061207 0.097573
Cell Periphery 0.012287 0.005124 0.034733 0.016113 0.007178 0.004045 0.010088 0.000948 0.010498 0.004602 0.012189 0.013007
Cytoplasm 0.846703* 0.842302* 0.441007* 0.556316* 0.762573* 0.751201* 0.749322* 0.836551* 0.817250* 0.798196* 0.723046* 0.613714*
Cytoplasmic Foci 0.030070 0.039023 0.086167 0.072655 0.070838 0.068033 0.031540 0.017065 0.044342 0.053068 0.036196 0.061269
Eisosomes 0.001772 0.000321 0.000449 0.000433 0.000825 0.000724 0.000219 0.000097 0.001441 0.000516 0.000239 0.000364
Endoplasmic Reticulum 0.002482 0.000671 0.003651 0.008411 0.003417 0.001417 0.001101 0.000006 0.002810 0.001032 0.001319 0.006690
Endosome 0.001905 0.002390 0.007741 0.006271 0.006284 0.006625 0.001623 0.000744 0.003438 0.004440 0.002144 0.005139
Golgi 0.005596 0.004910 0.005185 0.002334 0.004961 0.008893 0.001721 0.000295 0.005374 0.006838 0.002016 0.001916
Lipid Particles 0.003255 0.003100 0.064908 0.010932 0.003586 0.004853 0.018987 0.000624 0.003371 0.003948 0.022901 0.008821
Mitochondria 0.000632 0.000368 0.000014 0.000002 0.000193 0.001334 0.000024 0.000073 0.000478 0.000836 0.000023 0.000017
Mitotic Spindle 0.000468 0.000131 0.000546 0.000092 0.000084 0.001907 0.000156 0.000032 0.000334 0.000991 0.000190 0.000080
None 0.005869 0.001403 0.000204 0.000017 0.000595 0.000695 0.000586 0.000065 0.004023 0.001060 0.000554 0.000026
Nuclear Periphery 0.000040 0.000013 0.000095 0.000039 0.000027 0.000020 0.000037 0.000011 0.000036 0.000016 0.000042 0.000033
Nuclear Periphery Foci 0.001063 0.000633 0.028896 0.008983 0.002120 0.000730 0.008385 0.000263 0.001433 0.000680 0.010133 0.007197
Nucleolus 0.000257 0.000167 0.000025 0.000015 0.000206 0.000160 0.000076 0.000235 0.000239 0.000163 0.000071 0.000060
Nucleus 0.007635 0.004223 0.005100 0.001934 0.005146 0.005767 0.006363 0.003573 0.006764 0.004971 0.006255 0.002270
Peroxisomes 0.001308 0.001517 0.001402 0.001820 0.001319 0.003235 0.000917 0.003072 0.001312 0.002349 0.000958 0.002076
Vacuole 0.027113 0.028173 0.169583 0.177759 0.056201 0.056293 0.099473 0.075165 0.037297 0.041788 0.105448 0.156746
Vacuole Periphery 0.000626 0.000808 0.000426 0.000205 0.000869 0.001172 0.000159 0.000243 0.000711 0.000984 0.000182 0.000212

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.88 -6.51 -4.50 -4.16 3.14 -1.91 -5.79 3.08 6.94 11.03 -5.06 -12.97 -3.92 -3.22 -0.09
Bud Neck 6.25 -3.38 0.60 -2.92 3.15 -0.74 6.27 -1.73 -1.61 -2.75 3.55 10.59 -1.00 -2.33 -4.53
Bud Site -9.07 -9.40 -5.89 -4.51 -0.19 -2.57 0.43 2.61 3.57 2.48 -9.56 -7.49 -4.84 -3.40 -3.21
Cell Periphery 18.03* -4.56 -1.02 -3.71 3.32 6.98 -3.31 12.41 10.12 13.50 19.64 -1.30 -0.74 -3.41 -0.31
Cytoplasm 1.14 21.17 10.81 10.63 -3.71 1.82 2.86 -3.01 -3.50 -3.82 5.32 16.89 8.07 7.31 3.99
Cytoplasmic Foci -4.87 -7.28 -4.06 -3.25 1.00 0.77 13.12 5.38 5.17 1.64 -5.10 3.29 -2.04 -1.11 -2.70
Eisosomes 16.59 13.82 13.00 -1.39 0.24 1.52 10.26 10.49 12.60 3.12 14.74 19.83 14.02 3.24 -2.15
Endoplasmic Reticulum 3.45 -0.93 -1.25 -1.69 -1.02 2.74 3.25 4.83 7.42 6.21 4.15 3.46 -0.99 -1.54 -1.47
Endosome -2.02 -4.73 -4.72 -4.13 1.56 -0.53 8.95 9.99 13.15 3.05 -3.44 4.38 -1.97 -0.64 -3.63
Golgi 1.82 0.84 6.67 4.65 2.38 -5.75 5.63 19.07 12.80 2.32 -3.77 6.49 8.64 9.43 0.17
Lipid Particles 0.43 -8.96 -4.27 -4.29 7.54 -2.11 -13.18 6.96 6.99 15.70 -1.72 -15.75 -3.63 -3.19 7.12
Mitochondria 5.64 16.77 17.28 12.36 1.76 -3.65 3.05 2.01 4.10 -2.11 -2.33 14.72 14.71 5.48 0.92
Mitotic Spindle 1.59 -0.90 1.02 -0.53 1.63 -3.29 -1.67 2.09 3.38 2.56 -2.18 0.76 0.90 2.80 0.40
None 6.35 8.05 8.46 10.80 1.29 -1.00 0.20 6.24 8.36 3.70 6.45 7.45 8.83 13.74 4.11
Nuclear Periphery 17.51* -3.73 -0.02 -2.52 3.26 1.69 -2.33 3.61 4.06 11.62 10.30 -3.05 0.16 -2.05 1.08
Nuclear Periphery Foci 6.74 -10.20 -5.05 -5.33 6.37 7.93 -15.05 9.93 5.59 20.84* 10.17 -19.36 -4.42 -5.00 2.25
Nucleolus 6.13 16.22 17.23 26.48* 2.83 0.45 1.24 -0.19 -1.35 -2.98 1.96 4.33 4.32 6.90 0.66
Nucleus 8.75 4.20 10.49 4.60 4.72 -1.31 -3.10 1.93 2.92 4.02 5.82 0.91 9.77 6.53 10.02
Peroxisomes -2.87 -0.77 -2.44 -1.36 -1.81 -7.47 5.92 -1.37 0.19 -1.69 -7.97 7.36 -2.44 0.98 -3.61
Vacuole -0.98 -12.39 -7.73 -7.67 0.06 -0.08 -12.85 -1.43 -1.42 2.02 -3.55 -23.06 -7.31 -7.03 -2.79
Vacuole Periphery -4.76 1.57 14.10 16.34 1.40 -1.12 3.30 2.82 5.67 -1.48 -2.49 6.81 6.35 9.86 -1.17
Standard name
Human Ortholog
Description Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Rck2

Rck2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rck2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available