Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 38 | 33 | 27 | 61 | 16 | 35 | – | 45 | 37 | 28 | 28 | 22 | 49 | 17 | 17 | 12 | 19 | 9 | 14 | 6 | 30 | 52 | 26 |
Bud | 5 | 2 | 1 | 1 | 6 | 21 | 14 | – | 27 | 21 | 19 | 18 | 5 | 10 | 9 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 2 | 1 | 3 | 1 | – | 3 | 3 | 5 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 40 | 16 | 19 | 19 | 9 | 15 | – | 8 | 11 | 8 | 8 | 10 | 12 | 16 | 21 | 18 | 16 | 0 | 4 | 0 | 0 | 4 | 2 |
Endoplasmic Reticulum | 5 | 39 | 10 | 11 | 10 | 3 | 1 | – | 5 | 4 | 4 | 4 | 10 | 4 | 4 | 5 | 4 | 3 | 1 | 7 | 1 | 6 | 19 | 15 |
Endosome | 92 | 25 | 44 | 19 | 42 | 24 | 43 | – | 30 | 34 | 23 | 38 | 26 | 60 | 71 | 31 | 22 | 11 | 60 | 95 | 59 | 32 | 88 | 69 |
Golgi | 95 | 113 | 43 | 36 | 97 | 29 | 149 | – | 88 | 123 | 84 | 73 | 169 | 307 | 225 | 68 | 65 | 59 | 272 | 149 | 84 | 145 | 273 | 196 |
Mitochondria | 165 | 41 | 21 | 9 | 9 | 99 | 63 | – | 213 | 150 | 162 | 202 | 73 | 144 | 101 | 11 | 14 | 13 | 3 | 7 | 8 | 5 | 11 | 6 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 0 | 5 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 1 | 1 | – | 0 | 3 | 1 | 5 | 1 | 5 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 1 | 4 |
Nucleolus | 9 | 4 | 2 | 1 | 2 | 9 | 7 | – | 14 | 9 | 7 | 24 | 3 | 4 | 4 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 13 | 1 | 0 | 0 | 2 | 6 | 9 | – | 17 | 26 | 9 | 18 | 9 | 27 | 59 | 0 | 0 | 1 | 7 | 2 | 7 | 9 | 9 | 4 |
SpindlePole | 8 | 1 | 8 | 3 | 19 | 4 | 31 | – | 12 | 24 | 11 | 25 | 0 | 7 | 8 | 8 | 13 | 3 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 62 | 67 | 51 | 6 | 16 | 14 | 7 | – | 4 | 5 | 2 | 6 | 9 | 18 | 7 | 119 | 72 | 44 | 23 | 60 | 42 | 14 | 46 | 87 |
Unique Cell Count | 336 | 276 | 162 | 98 | 199 | 172 | 272 | 319 | 304 | 252 | 318 | 222 | 450 | 387 | 220 | 164 | 127 | 383 | 351 | 215 | 248 | 514 | 422 | |
Labelled Cell Count | 475 | 371 | 231 | 135 | 285 | 238 | 381 | 467 | 455 | 363 | 456 | 337 | 649 | 522 | 290 | 227 | 178 | 383 | 351 | 215 | 248 | 514 | 422 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.2 | 11.5 | 9.9 | 10.7 | 11.7 | 8.2 | 11.3 | – | 10.6 | 11.0 | 11.3 | 10.9 | 12.6 | 12.8 | 13.0 | 13.8 | 14.5 | 14.4 | 11.2 | 11.7 | 11.2 |
Std Deviation (1e-4) | 2.4 | 2.1 | 1.9 | 1.9 | 2.1 | 2.2 | 2.2 | – | 2.2 | 2.5 | 2.6 | 2.7 | 2.2 | 2.8 | 3.2 | 2.9 | 3.5 | 3.1 | 3.6 | 3.6 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.25 | -0.27 | 0.2 | – | 0.1 | 0.16 | 0.2 | 0.14 | 0.35 | 0.37 | 0.39 | 0.48 | 0.56 | 0.54 | 0.18 | 0.24 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.3 | 2.2 | -2.9 | -2.1 | – | -1.8 | -2.4 | -2.6 | -3.6 | -2.9 | -3.0 | -5.9 | -3.6 | -3.4 | -1.2 |
Bud | 0 | 0 | 4.3 | 2.5 | – | 3.5 | 3.0 | 3.2 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | -0.1 | -1.6 | -1.7 | – | -3.5 | -2.8 | -2.8 | -3.5 | -2.1 | -3.8 | -2.6 | -0.1 | 0.3 | 0.7 |
Endoplasmic Reticulum | 1.4 | -0.5 | -2.1 | 0 | – | -2.7 | 0 | -2.5 | 0 | -0.7 | 0 | 0 | -1.9 | -1.7 | -1.6 |
Endosome | -1.4 | -1.3 | -3.0 | -2.9 | – | -5.1 | -4.4 | -4.9 | -4.2 | -3.9 | -4.0 | -2.3 | -3.2 | -3.1 | -4.0 |
Golgi | 1.7 | 4.3 | -2.2 | 5.7 | – | 0.2 | 3.0 | 1.5 | -0.9 | 9.6 | 9.2 | 6.8 | 0.9 | 2.5 | 3.5 |
Mitochondria | -0.9 | -2.9 | 8.5 | 2.6 | – | 11.2 | 7.8 | 10.3 | 10.5 | 4.5 | 4.7 | 3.4 | -2.8 | -1.3 | -0.7 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.0 | 0 | – | 1.8 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 3.0 | 3.8 | 0 | 3.1 | 0 | 3.2 | 5.3 | 0 | 0 | 0 |
SpindlePole | 0 | 1.7 | -1.3 | 2.3 | – | -0.6 | 1.2 | -0.3 | 1.2 | 0 | 0 | 0 | -0.6 | 1.1 | 0 |
Vacuole | -4.8 | -5.7 | -5.4 | -8.6 | – | -9.8 | -9.4 | -9.1 | -9.5 | -7.3 | -9.5 | -10.3 | 4.4 | 2.3 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.9709 | 11.4166 | 9.9118 | 9.5172 | 10.2904 | 10.9361 | 9.9355 | 13.2107 | 11.335 | 11.0009 | 9.1289 | 12.0075 | 16.2497 | 17.4466 | 16.8813 | 15.6009 | 16.0538 | 16.9012 |
Actin | 0.0406 | 0.0014 | 0.0095 | 0.0142 | 0.0003 | 0.0022 | 0.0575 | 0.007 | 0.0034 | 0.0099 | 0.0491 | 0.0096 | 0.0101 | 0.0093 | 0.0082 | 0.0104 | 0.0011 | 0.0101 |
Bud | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0043 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0006 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0002 | 0.0008 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0032 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0014 | 0 | 0.0003 | 0.0004 | 0 | 0 | 0.0022 | 0.0001 | 0.0057 | 0.0004 | 0.0023 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0034 | 0.0006 | 0.0032 | 0.0004 | 0.0014 | 0.0006 | 0.0054 | 0.002 | 0.002 | 0.0014 | 0.0022 | 0.0015 | 0.0008 | 0.0017 | 0.0007 | 0.0009 | 0.0004 | 0.0007 |
Cytoplasmic Foci | 0.0456 | 0.0389 | 0.0336 | 0.0298 | 0.0213 | 0.0199 | 0.0704 | 0.058 | 0.0527 | 0.0446 | 0.0662 | 0.0401 | 0.048 | 0.0459 | 0.0353 | 0.0529 | 0.029 | 0.0276 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0004 | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0087 | 0.0015 | 0.0012 | 0.0007 | 0.0013 | 0.0007 | 0.0007 | 0.0007 | 0.0003 | 0.0003 | 0.0023 | 0.0006 |
Endosome | 0.1053 | 0.1602 | 0.1504 | 0.2201 | 0.1494 | 0.1541 | 0.169 | 0.2213 | 0.1661 | 0.2759 | 0.1733 | 0.1486 | 0.1558 | 0.2468 | 0.1446 | 0.2774 | 0.1544 | 0.1374 |
Golgi | 0.7104 | 0.7746 | 0.7623 | 0.6426 | 0.7836 | 0.7961 | 0.5733 | 0.6687 | 0.7246 | 0.5979 | 0.52 | 0.7557 | 0.7405 | 0.6654 | 0.7968 | 0.6304 | 0.7554 | 0.8109 |
Lipid Particles | 0.03 | 0.008 | 0.0035 | 0.0072 | 0.0014 | 0.0026 | 0.0221 | 0.0103 | 0.0071 | 0.0049 | 0.0841 | 0.0072 | 0.0107 | 0.0087 | 0.0033 | 0.0017 | 0.0026 | 0.0027 |
Mitochondria | 0.027 | 0.001 | 0.0241 | 0.0082 | 0.0014 | 0.0027 | 0.0379 | 0.0049 | 0.0175 | 0.0064 | 0.0619 | 0.0251 | 0.0139 | 0.0022 | 0.0042 | 0.0014 | 0.0015 | 0.003 |
None | 0.0036 | 0 | 0.001 | 0 | 0 | 0.0083 | 0.0026 | 0 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0091 | 0.0005 | 0.0003 | 0.0004 | 0.0192 | 0.0004 | 0.0046 | 0.0008 | 0.0004 | 0.0005 | 0.0033 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0018 | 0.0001 |
Nucleolus | 0.0014 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0.0045 | 0 | 0.0002 | 0 | 0.0013 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0025 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0061 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Peroxisomes | 0.0064 | 0.0004 | 0.0033 | 0.0205 | 0.0001 | 0.0002 | 0.0173 | 0.0008 | 0.0033 | 0.0072 | 0.0133 | 0.0034 | 0.0048 | 0.002 | 0.0013 | 0.0074 | 0.0002 | 0.0005 |
Punctate Nuclear | 0.0027 | 0 | 0.0012 | 0.0002 | 0.0001 | 0.0024 | 0.0073 | 0 | 0.0001 | 0.0002 | 0.0018 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0024 | 0.007 | 0.0016 | 0.0206 | 0.0052 | 0.0028 | 0.004 | 0.011 | 0.0047 | 0.0185 | 0.004 | 0.0026 | 0.0057 | 0.0071 | 0.0015 | 0.0083 | 0.0291 | 0.0021 |
Vacuole Periphery | 0.0039 | 0.0069 | 0.0042 | 0.0338 | 0.0157 | 0.0058 | 0.0048 | 0.0133 | 0.0088 | 0.0308 | 0.0052 | 0.0031 | 0.0074 | 0.0093 | 0.0032 | 0.0083 | 0.0219 | 0.0036 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.1266 | 71.3549 | 69.5597 | 70.0246 | 57.8333 | 68.8268 | 65.4273 | 72.0866 | 90.209 | 67.8155 |
Translational Efficiency | 1.9112 | 1.3967 | 1.7354 | 2.0418 | 1.5921 | 1.9658 | 1.6265 | 1.6608 | 1.4635 | 1.581 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1996 | 1619 | 1881 | 1769 | 1531 | 1800 | 1652 | 1364 | 3527 | 3419 | 3533 | 3133 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1047.81 | 1255.88 | 1565.78 | 1055.62 | 973.29 | 1196.26 | 1348.23 | 1161.79 | 1015.46 | 1224.49 | 1464.06 | 1101.84 |
Standard Deviation | 150.81 | 194.66 | 241.31 | 226.60 | 145.35 | 321.14 | 213.56 | 239.55 | 152.99 | 270.42 | 253.20 | 238.22 |
Intensity Change Log 2 | — | 0.261321 | 0.579504 | 0.010713 | — | 0.297589 | 0.470125 | 0.255408 | — | 0.278901 | 0.527865 | 0.133735 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.011071 | 0.018609 | 0.026368 | 0.015705 | 0.013937 | 0.011781 | 0.016853 | 0.005287 | 0.012315 | 0.015015 | 0.021919 | 0.011169 |
Bud Neck | 0.000164 | 0.000220 | 0.000789 | 0.001840 | 0.000155 | 0.000271 | 0.000513 | 0.000544 | 0.000160 | 0.000247 | 0.000660 | 0.001276 |
Bud Site | 0.001381 | 0.001277 | 0.001413 | 0.006544 | 0.000973 | 0.002035 | 0.002135 | 0.003898 | 0.001204 | 0.001676 | 0.001751 | 0.005392 |
Cell Periphery | 0.000090 | 0.000214 | 0.000141 | 0.000100 | 0.000070 | 0.000199 | 0.000097 | 0.000103 | 0.000081 | 0.000206 | 0.000121 | 0.000102 |
Cytoplasm | 0.000393 | 0.001219 | 0.000415 | 0.006239 | 0.000573 | 0.001356 | 0.000898 | 0.002263 | 0.000471 | 0.001291 | 0.000641 | 0.004508 |
Cytoplasmic Foci | 0.165324 | 0.138451 | 0.114083 | 0.268937* | 0.284809* | 0.153780 | 0.202534* | 0.320290* | 0.217190* | 0.146521 | 0.155442 | 0.291294* |
Eisosomes | 0.000103 | 0.000141 | 0.000151 | 0.000069 | 0.000089 | 0.000094 | 0.000099 | 0.000026 | 0.000097 | 0.000116 | 0.000127 | 0.000050 |
Endoplasmic Reticulum | 0.000491 | 0.001830 | 0.002872 | 0.000795 | 0.000521 | 0.000615 | 0.001394 | 0.000432 | 0.000504 | 0.001190 | 0.002181 | 0.000637 |
Endosome | 0.255007* | 0.195870 | 0.128165 | 0.347289* | 0.185374 | 0.307182* | 0.150615 | 0.268580* | 0.224781* | 0.254473* | 0.138662 | 0.313022* |
Golgi | 0.521888* | 0.574658* | 0.564103* | 0.224013* | 0.462554* | 0.446965* | 0.556503* | 0.335628* | 0.496133* | 0.507431* | 0.560549* | 0.272607* |
Lipid Particles | 0.014228 | 0.028236 | 0.140026* | 0.055607 | 0.033383 | 0.043674 | 0.049932 | 0.021767 | 0.022543 | 0.036364 | 0.097899 | 0.040874 |
Mitochondria | 0.011443 | 0.006005 | 0.000678 | 0.003623 | 0.005187 | 0.008618 | 0.002342 | 0.001558 | 0.008727 | 0.007381 | 0.001456 | 0.002724 |
Mitotic Spindle | 0.000161 | 0.000541 | 0.003593 | 0.026891 | 0.000396 | 0.000176 | 0.001212 | 0.016158 | 0.000263 | 0.000349 | 0.002480 | 0.022218 |
None | 0.000014 | 0.000053 | 0.000035 | 0.000513 | 0.000091 | 0.000494 | 0.000018 | 0.000068 | 0.000047 | 0.000285 | 0.000027 | 0.000319 |
Nuclear Periphery | 0.000074 | 0.000044 | 0.000337 | 0.000220 | 0.000016 | 0.000053 | 0.000131 | 0.000096 | 0.000049 | 0.000048 | 0.000241 | 0.000166 |
Nuclear Periphery Foci | 0.000386 | 0.000376 | 0.002049 | 0.005187 | 0.000101 | 0.000305 | 0.000527 | 0.001983 | 0.000262 | 0.000338 | 0.001337 | 0.003792 |
Nucleolus | 0.000003 | 0.000006 | 0.000017 | 0.001318 | 0.000005 | 0.000047 | 0.000004 | 0.000059 | 0.000004 | 0.000028 | 0.000011 | 0.000770 |
Nucleus | 0.000007 | 0.000026 | 0.000045 | 0.001012 | 0.000009 | 0.000413 | 0.000013 | 0.000053 | 0.000008 | 0.000230 | 0.000030 | 0.000594 |
Peroxisomes | 0.004344 | 0.001887 | 0.000733 | 0.012465 | 0.007172 | 0.005035 | 0.002078 | 0.005204 | 0.005572 | 0.003544 | 0.001362 | 0.009304 |
Vacuole | 0.008228 | 0.020639 | 0.009854 | 0.018424 | 0.002426 | 0.010716 | 0.008654 | 0.013561 | 0.005709 | 0.015415 | 0.009293 | 0.016307 |
Vacuole Periphery | 0.005199 | 0.009699 | 0.004131 | 0.003206 | 0.002160 | 0.006192 | 0.003448 | 0.002442 | 0.003880 | 0.007853 | 0.003812 | 0.002874 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.35 | -6.17 | -1.81 | 1.04 | 3.49 | 1.01 | -1.26 | 4.50 | 3.45 | 5.45 | -1.79 | -5.64 | 0.73 | 2.17 | 5.51 |
Bud Neck | -1.93 | -3.59 | -5.54 | -5.36 | -3.06 | -2.00 | -5.50 | -4.84 | -3.35 | -0.64 | -2.67 | -5.18 | -6.47 | -5.94 | -3.20 |
Bud Site | 0.26 | -0.15 | -5.97 | -6.65 | -6.39 | -1.79 | -3.47 | -5.32 | -2.73 | -2.96 | -1.26 | -2.20 | -7.75 | -6.58 | -6.92 |
Cell Periphery | -4.28 | -4.35 | -0.83 | 3.69 | 2.66 | -1.15 | -1.84 | -1.08 | 0.74 | -0.38 | -2.06 | -4.24 | -1.33 | 1.59 | 0.78 |
Cytoplasm | -1.37 | -0.70 | -5.93 | -4.47 | -5.82 | -1.70 | -1.37 | -3.38 | -1.51 | -2.19 | -2.19 | -1.57 | -6.76 | -4.74 | -6.14 |
Cytoplasmic Foci | 5.96 | 11.87 | -18.23 | -22.74 | -27.69* | 24.38 | 14.10 | -4.98 | -25.51 | -17.02 | 19.67 | 16.46 | -16.18 | -33.44 | -30.44 |
Eisosomes | -3.33 | -4.39 | 3.47 | 6.42 | 7.69 | -0.64 | -1.18 | 13.07 | 11.28 | 9.57 | -2.80 | -4.15 | 7.85 | 10.10 | 11.19 |
Endoplasmic Reticulum | -5.37 | -9.71 | -1.54 | 3.38 | 6.87 | -0.59 | -4.70 | 0.79 | 1.14 | 5.24 | -4.74 | -10.67 | -1.13 | 3.14 | 8.31 |
Endosome | 9.57 | 23.10 | -12.05 | -20.32 | -32.04* | -19.08 | 6.82 | -12.67 | 5.33 | -18.82 | -6.37 | 22.33 | -16.79 | -10.55 | -36.48* |
Golgi | -6.65 | -4.93 | 38.79* | 41.71* | 38.29* | 2.01 | -11.39 | 14.51 | 12.27 | 23.27* | -1.97 | -10.76 | 38.02 | 37.25 | 44.04* |
Lipid Particles | -5.51 | -23.62 | -12.05 | -7.20 | 13.81 | -2.56 | -3.99 | 3.34 | 6.02 | 7.46 | -5.90 | -21.54 | -7.52 | -1.76 | 14.91 |
Mitochondria | 3.24 | 8.22 | 5.58 | 1.99 | -5.23 | -2.38 | 2.54 | 3.09 | 5.66 | 0.92 | 1.18 | 8.15 | 6.36 | 5.40 | -2.57 |
Mitotic Spindle | -1.21 | -3.67 | -11.00 | -10.76 | -8.99 | 2.88 | -3.04 | -7.67 | -7.77 | -7.23 | -0.57 | -4.32 | -13.38 | -13.28 | -11.53 |
None | -1.24 | -1.15 | -2.64 | -2.45 | -2.33 | -1.14 | 0.94 | 0.27 | 1.24 | -1.73 | -1.29 | 0.03 | -2.46 | -0.39 | -2.51 |
Nuclear Periphery | 0.76 | -4.16 | -3.19 | -6.59 | 1.95 | -6.58 | -10.61 | -6.99 | -3.59 | 2.06 | 0.03 | -5.47 | -4.40 | -7.34 | 2.21 |
Nuclear Periphery Foci | 0.03 | -3.41 | -6.90 | -7.00 | -4.08 | -4.09 | -5.19 | -3.57 | -3.18 | -2.76 | -0.52 | -4.03 | -7.75 | -7.71 | -5.03 |
Nucleolus | -2.59 | -1.03 | -3.16 | -3.15 | -3.05 | -1.08 | 0.19 | -3.50 | -0.39 | -3.54 | -1.16 | -1.03 | -3.24 | -3.15 | -3.15 |
Nucleus | -1.31 | -1.07 | -3.67 | -3.60 | -3.05 | -1.09 | -1.62 | -2.47 | 0.97 | -2.07 | -1.13 | -1.13 | -3.80 | -1.27 | -3.20 |
Peroxisomes | 4.42 | 7.06 | -6.81 | -9.17 | -10.26 | 1.79 | 5.33 | 1.58 | -0.28 | -4.07 | 3.14 | 8.27 | -4.71 | -7.17 | -10.87 |
Vacuole | -4.91 | -1.28 | -5.67 | 0.78 | -5.39 | -6.93 | -8.02 | -9.08 | -1.91 | -3.60 | -6.96 | -4.56 | -9.26 | -0.69 | -6.51 |
Vacuole Periphery | -4.58 | 2.03 | 3.58 | 7.12 | 2.50 | -7.38 | -3.36 | -0.76 | 7.47 | 3.15 | -7.09 | 0.19 | 2.82 | 9.74 | 3.74 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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