Standard name | |
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Human Ortholog | |
Description | Mitochondrial protein of unknown function involved in lipid homeostasis; associates with mitochondrial ribosome; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 2 | 3 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 5 | 3 | 4 | 1 | 5 | 1 | 4 | 1 | 1 | 3 | 7 |
Bud | 3 | 7 | 1 | 5 | 0 | 3 | 3 | 7 | 4 | 5 | 8 | 10 | 1 | 3 | 3 | 6 | 6 | 10 | 2 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 4 | 17 | 4 | 10 | 4 | 2 | 0 | 1 | 0 | 5 | 5 | 1 | 14 | 7 | 7 | 18 | 16 | 12 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 8 | 3 | 9 | 12 | 14 | 18 | 26 | 16 | 9 | 13 | 14 | 13 | 16 | 25 | 8 | 6 | 10 | 0 | 1 | 0 | 5 | 0 | 1 |
Endoplasmic Reticulum | 3 | 3 | 0 | 1 | 3 | 3 | 1 | 1 | 3 | 1 | 8 | 1 | 4 | 8 | 3 | 6 | 2 | 7 | 0 | 1 | 0 | 0 | 1 | 3 |
Endosome | 2 | 8 | 3 | 8 | 14 | 26 | 28 | 32 | 9 | 13 | 0 | 11 | 11 | 21 | 41 | 11 | 11 | 16 | 11 | 10 | 6 | 8 | 7 | 9 |
Golgi | 3 | 22 | 46 | 40 | 51 | 4 | 4 | 10 | 5 | 7 | 4 | 3 | 75 | 113 | 108 | 59 | 59 | 31 | 9 | 28 | 6 | 16 | 23 | 37 |
Mitochondria | 280 | 253 | 57 | 174 | 119 | 253 | 243 | 356 | 277 | 268 | 357 | 311 | 150 | 160 | 156 | 14 | 11 | 18 | 250 | 244 | 129 | 109 | 158 | 207 |
Nucleus | 1 | 1 | 2 | 2 | 1 | 5 | 14 | 15 | 8 | 7 | 6 | 8 | 1 | 1 | 1 | 4 | 4 | 4 | 0 | 1 | 2 | 1 | 2 | 1 |
Nuclear Periphery | 17 | 10 | 1 | 1 | 4 | 20 | 11 | 32 | 22 | 10 | 23 | 12 | 0 | 2 | 1 | 3 | 2 | 1 | 4 | 2 | 0 | 2 | 1 | 2 |
Nucleolus | 16 | 4 | 0 | 2 | 4 | 18 | 17 | 29 | 15 | 13 | 10 | 16 | 2 | 2 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 1 |
Peroxisomes | 1 | 10 | 4 | 7 | 1 | 4 | 7 | 8 | 2 | 5 | 0 | 3 | 3 | 6 | 27 | 8 | 6 | 12 | 7 | 4 | 5 | 9 | 9 | 9 |
SpindlePole | 1 | 3 | 3 | 4 | 7 | 6 | 9 | 21 | 5 | 8 | 3 | 15 | 1 | 5 | 7 | 7 | 2 | 17 | 1 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 29 | 53 | 37 | 18 | 33 | 56 | 57 | 56 | 33 | 35 | 19 | 31 | 54 | 42 | 57 | 42 | 21 | 34 | 8 | 13 | 3 | 10 | 16 | 28 |
Unique Cell Count | 294 | 299 | 111 | 219 | 199 | 337 | 335 | 497 | 328 | 321 | 386 | 370 | 221 | 277 | 326 | 147 | 122 | 138 | 299 | 319 | 160 | 170 | 232 | 320 |
Labelled Cell Count | 363 | 399 | 162 | 283 | 257 | 416 | 414 | 598 | 402 | 389 | 458 | 436 | 330 | 391 | 441 | 192 | 149 | 182 | 299 | 319 | 160 | 170 | 232 | 320 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 7.5 | 8.4 | 8.0 | 8.4 | 7.2 | 7.2 | 7.4 | 6.3 | 6.6 | 5.9 | 6.2 | 8.4 | 8.4 | 8.1 | 8.4 | 8.3 | 7.5 | 8.1 | 8.4 | 8.9 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.3 | 1.7 | 1.6 | 1.5 | 1.8 | 1.7 | 1.4 | 1.5 | 1.5 | 1.5 | 1.1 | 1.2 | 1.3 | 1.8 | 1.8 | 1.3 | 1.9 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.0 | -0.22 | -0.22 | -0.19 | -0.42 | -0.34 | -0.51 | -0.43 | 0.0 | 0.0 | -0.05 | 0.01 | -0.01 | -0.15 | -0.05 | 0.0 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 | 2.5 | 2.6 | 1.6 |
Cytoplasm | 0 | 1.3 | 0 | 1.2 | 1.1 | 0 | 0 | 0 | 0 | 1.3 | 1.3 | 1.8 | 0 | 0 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.6 | 1.9 | 2.0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 1.8 | 3.0 | 1.7 | 2.0 | 2.6 |
Golgi | -4.5 | -2.9 | -11.7 | -11.6 | -13.0 | -11.3 | -10.9 | -12.5 | -12.4 | -1.3 | -0.1 | -1.6 | -0.2 | 1.1 | -3.2 |
Mitochondria | 5.3 | 1.4 | 4.7 | 4.1 | 4.1 | 7.1 | 6.8 | 10.2 | 7.1 | 2.9 | 1.1 | -0.6 | -7.4 | -7.1 | -6.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.2 | 0 | 2.3 | 2.4 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0.7 | 0 | 1.6 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 |
Vacuole | -5.8 | -3.4 | -3.8 | -3.7 | -5.8 | -5.8 | -5.5 | -8.3 | -6.6 | -1.7 | -4.0 | -3.5 | -0.8 | -2.8 | -1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7267 | 2.8116 | 3.0177 | 3.0051 | 2.5713 | 3.0606 | 2.7518 | 2.6295 | 2.3566 | 2.1649 | 3.2916 | 2.9821 | 2.7761 | 2.8519 | 2.9088 | 2.8119 | 2.3325 | 2.5124 |
Actin | 0.0017 | 0.0029 | 0.0006 | 0.0007 | 0.0004 | 0.0006 | 0.0149 | 0.0104 | 0.018 | 0.0082 | 0.0708 | 0.0249 | 0.0127 | 0.0171 | 0.0049 | 0.0114 | 0.0023 | 0.0106 |
Bud | 0.0003 | 0.0006 | 0.004 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0028 | 0.0006 | 0.0008 | 0.0019 | 0.0024 | 0.0013 | 0.0019 | 0.0017 | 0.0005 | 0.0014 | 0.0007 |
Bud Neck | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0021 | 0.0014 | 0.0011 | 0.0007 | 0.0028 | 0.0014 | 0.0011 | 0.0018 | 0.0011 | 0.0005 | 0.0002 | 0.0008 |
Bud Periphery | 0.0014 | 0.0014 | 0.0015 | 0.001 | 0.0006 | 0.0005 | 0.0016 | 0.0059 | 0.0009 | 0.0028 | 0.0041 | 0.0078 | 0.0021 | 0.0028 | 0.0028 | 0.0011 | 0.0026 | 0.0026 |
Bud Site | 0.0005 | 0.0013 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0058 | 0.019 | 0.0055 | 0.0069 | 0.0138 | 0.0015 | 0.0043 | 0.0312 | 0.0168 | 0.0017 | 0.0011 | 0.0009 |
Cell Periphery | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0012 | 0.0065 | 0.0006 | 0.0005 | 0.0012 | 0.0005 | 0.0008 | 0.0022 | 0.0011 | 0.0002 | 0.0002 | 0.0012 |
Cytoplasm | 0.001 | 0.001 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0.0047 | 0.0048 | 0.0149 | 0.0004 | 0.0048 | 0.0141 | 0.0114 | 0.0056 | 0.0089 | 0.0011 | 0.0066 | 0.0007 |
Cytoplasmic Foci | 0.0097 | 0.006 | 0.0037 | 0.0055 | 0.0034 | 0.0025 | 0.0205 | 0.0294 | 0.0202 | 0.0051 | 0.0228 | 0.0134 | 0.0333 | 0.0276 | 0.0131 | 0.0122 | 0.0151 | 0.003 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0022 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0006 |
Endoplasmic Reticulum | 0.0012 | 0.0008 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0145 | 0.0027 | 0.0012 | 0.0006 | 0.0047 | 0.0013 | 0.0052 | 0.0014 | 0.0036 | 0.0003 | 0.002 | 0.0008 |
Endosome | 0.0478 | 0.0418 | 0.0168 | 0.0216 | 0.0136 | 0.0156 | 0.0449 | 0.1049 | 0.0314 | 0.0323 | 0.1464 | 0.0419 | 0.0701 | 0.0639 | 0.0319 | 0.0216 | 0.0468 | 0.0285 |
Golgi | 0.0316 | 0.0355 | 0.0143 | 0.0192 | 0.0223 | 0.0185 | 0.0334 | 0.0655 | 0.0264 | 0.0106 | 0.0443 | 0.0484 | 0.0414 | 0.0405 | 0.0221 | 0.0317 | 0.0126 | 0.0393 |
Lipid Particles | 0.0227 | 0.0175 | 0.0067 | 0.0011 | 0.001 | 0.0123 | 0.0435 | 0.0157 | 0.017 | 0.0293 | 0.0203 | 0.0179 | 0.0382 | 0.0145 | 0.0056 | 0.0119 | 0.012 | 0.005 |
Mitochondria | 0.8306 | 0.8608 | 0.9316 | 0.9103 | 0.9407 | 0.9303 | 0.7449 | 0.6521 | 0.8282 | 0.8602 | 0.5329 | 0.7827 | 0.7234 | 0.7326 | 0.8496 | 0.8825 | 0.8458 | 0.8879 |
None | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.015 | 0.0029 | 0.0004 | 0.0004 | 0.0031 | 0.0022 | 0.0045 | 0.0039 | 0.0011 | 0.0009 | 0.009 | 0.0003 |
Nuclear Periphery | 0.0017 | 0.0005 | 0.0006 | 0 | 0 | 0.0003 | 0.0121 | 0.002 | 0.0002 | 0.0008 | 0.0459 | 0.0107 | 0.0126 | 0.0015 | 0.0009 | 0.0001 | 0.004 | 0.0014 |
Nucleolus | 0.0026 | 0.0006 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.002 | 0.0022 | 0.0033 | 0.001 | 0.0013 | 0.0006 | 0.0019 | 0.0027 | 0.0007 | 0.0001 | 0.0007 | 0.0002 |
Nucleus | 0.0007 | 0.0004 | 0.001 | 0 | 0.0001 | 0.0004 | 0.0058 | 0.0015 | 0.0006 | 0.0007 | 0.02 | 0.0016 | 0.0019 | 0.0015 | 0.0006 | 0.0001 | 0.0013 | 0.0003 |
Peroxisomes | 0.024 | 0.007 | 0.0103 | 0.0358 | 0.0138 | 0.0105 | 0.012 | 0.0294 | 0.0216 | 0.0049 | 0.0339 | 0.0162 | 0.0154 | 0.0139 | 0.0108 | 0.0166 | 0.0162 | 0.0032 |
Punctate Nuclear | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.005 | 0.0003 | 0.0006 | 0.0005 | 0.005 | 0.0008 | 0.0014 | 0.0005 | 0.0003 | 0.0002 | 0.0091 | 0.0002 |
Vacuole | 0.009 | 0.0109 | 0.0019 | 0.0011 | 0.0011 | 0.0021 | 0.0089 | 0.0265 | 0.0033 | 0.0122 | 0.0086 | 0.0033 | 0.0091 | 0.0216 | 0.0117 | 0.0021 | 0.0067 | 0.0059 |
Vacuole Periphery | 0.0118 | 0.0097 | 0.0035 | 0.0022 | 0.002 | 0.0038 | 0.0059 | 0.0119 | 0.0034 | 0.0207 | 0.0106 | 0.0061 | 0.0064 | 0.011 | 0.0102 | 0.0029 | 0.0041 | 0.006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.26 | 10.3829 | 15.8372 | 15.0146 | 12.9675 | 18.0574 | 16.9137 | 18.5011 | 19.7258 | 19.6228 |
Translational Efficiency | 0.5618 | 0.7062 | 0.4371 | 0.6015 | 0.4919 | 0.431 | 0.6505 | 0.4234 | 0.4488 | 0.4223 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
847 | 1380 | 192 | 269 | 2141 | 1530 | 319 | 1883 | 2988 | 2910 | 511 | 2152 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 611.74 | 873.23 | 1020.31 | 943.75 | 792.56 | 841.52 | 1044.33 | 822.48 | 741.30 | 856.56 | 1035.30 | 837.64 |
Standard Deviation | 74.26 | 152.42 | 111.35 | 115.23 | 116.67 | 116.87 | 120.61 | 100.56 | 134.01 | 135.83 | 117.79 | 110.07 |
Intensity Change Log 2 | — | 0.513443 | 0.738017 | 0.625486 | — | 0.086477 | 0.397986 | 0.053460 | — | 0.288147 | 0.556039 | 0.330822 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000387 | 0.000897 | 0.003569 | 0.012800 | 0.000505 | 0.000583 | 0.006830 | 0.000252 | 0.000471 | 0.000732 | 0.005604 | 0.001821 |
Bud Neck | 0.028186 | 0.002071 | 0.019027 | 0.016947 | 0.001995 | 0.000947 | 0.006076 | 0.030404 | 0.009420 | 0.001480 | 0.010942 | 0.028722 |
Bud Site | 0.028507 | 0.009735 | 0.033762 | 0.092181 | 0.005114 | 0.003927 | 0.047825 | 0.038126 | 0.011745 | 0.006681 | 0.042541 | 0.044883 |
Cell Periphery | 0.001604 | 0.000631 | 0.000501 | 0.000953 | 0.000777 | 0.000371 | 0.000411 | 0.000781 | 0.001012 | 0.000494 | 0.000445 | 0.000802 |
Cytoplasm | 0.018897 | 0.001978 | 0.003142 | 0.011013 | 0.000940 | 0.000839 | 0.007633 | 0.014049 | 0.006030 | 0.001379 | 0.005945 | 0.013669 |
Cytoplasmic Foci | 0.044064 | 0.017991 | 0.065633 | 0.041842 | 0.011188 | 0.017601 | 0.090726 | 0.023141 | 0.020508 | 0.017786 | 0.081298 | 0.025479 |
Eisosomes | 0.000129 | 0.000046 | 0.000099 | 0.000121 | 0.000037 | 0.000037 | 0.000093 | 0.000096 | 0.000063 | 0.000041 | 0.000096 | 0.000099 |
Endoplasmic Reticulum | 0.000868 | 0.000363 | 0.003836 | 0.001311 | 0.000354 | 0.000254 | 0.001966 | 0.001550 | 0.000500 | 0.000306 | 0.002669 | 0.001520 |
Endosome | 0.072833 | 0.052059 | 0.147476 | 0.092771 | 0.036354 | 0.051310 | 0.130202 | 0.145388 | 0.046695 | 0.051665 | 0.136692 | 0.138811 |
Golgi | 0.059456 | 0.043160 | 0.080759 | 0.073835 | 0.029253 | 0.049940 | 0.158509* | 0.034391 | 0.037814 | 0.046724 | 0.129296* | 0.039321 |
Lipid Particles | 0.006597 | 0.001208 | 0.004102 | 0.000509 | 0.002084 | 0.002271 | 0.001093 | 0.002519 | 0.003363 | 0.001767 | 0.002224 | 0.002268 |
Mitochondria | 0.627005* | 0.819861* | 0.373944* | 0.418483* | 0.867407* | 0.819190* | 0.302849* | 0.424460* | 0.799261* | 0.819509* | 0.329562* | 0.423713* |
Mitotic Spindle | 0.006989 | 0.005962 | 0.089061 | 0.074496 | 0.002693 | 0.003986 | 0.097221 | 0.034028 | 0.003911 | 0.004923 | 0.094155 | 0.039087 |
None | 0.007760 | 0.000371 | 0.000331 | 0.000509 | 0.000281 | 0.000285 | 0.000270 | 0.002645 | 0.002401 | 0.000325 | 0.000293 | 0.002378 |
Nuclear Periphery | 0.000356 | 0.000204 | 0.003221 | 0.000495 | 0.000095 | 0.000113 | 0.000261 | 0.000697 | 0.000169 | 0.000156 | 0.001373 | 0.000672 |
Nuclear Periphery Foci | 0.000213 | 0.000315 | 0.005777 | 0.001481 | 0.000144 | 0.000157 | 0.000868 | 0.000750 | 0.000163 | 0.000232 | 0.002713 | 0.000841 |
Nucleolus | 0.003457 | 0.000110 | 0.005585 | 0.001412 | 0.000177 | 0.000411 | 0.000374 | 0.006633 | 0.001107 | 0.000268 | 0.002332 | 0.005980 |
Nucleus | 0.005389 | 0.000185 | 0.009530 | 0.002864 | 0.000176 | 0.000206 | 0.001311 | 0.008922 | 0.001653 | 0.000196 | 0.004399 | 0.008164 |
Peroxisomes | 0.027483 | 0.015360 | 0.066938 | 0.046324 | 0.010204 | 0.010653 | 0.048457 | 0.019351 | 0.015102 | 0.012885 | 0.055401 | 0.022723 |
Vacuole | 0.026275 | 0.013113 | 0.047265 | 0.065350 | 0.011583 | 0.016197 | 0.051095 | 0.137688 | 0.015748 | 0.014734 | 0.049656 | 0.128645 |
Vacuole Periphery | 0.033544 | 0.014379 | 0.036441 | 0.044302 | 0.018639 | 0.020724 | 0.045929 | 0.074130 | 0.022864 | 0.017715 | 0.042364 | 0.070401 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.25 | -4.29 | -3.02 | -2.90 | -2.22 | -0.41 | -3.80 | 1.60 | 2.90 | 3.96 | -1.60 | -4.74 | -2.55 | -2.06 | 3.19 |
Bud Neck | 7.63 | 1.58 | 2.53 | -5.77 | 0.29 | 2.20 | -4.30 | -14.36 | -15.18 | -11.50 | 7.44 | -0.71 | -9.64 | -15.64 | -6.97 |
Bud Site | 5.95 | -0.80 | -6.21 | -8.21 | -5.04 | 1.27 | -7.60 | -15.35 | -16.30 | 1.67 | 4.04 | -7.23 | -13.87 | -16.37 | -0.47 |
Cell Periphery | 2.06 | 2.38 | 1.28 | -1.09 | -1.61 | 1.41 | 1.37 | -0.01 | -3.01 | -4.20 | 2.11 | 2.42 | 0.89 | -2.65 | -4.02 |
Cytoplasm | 6.41 | 5.93 | 2.35 | -4.45 | -3.84 | 0.47 | -5.50 | -9.15 | -9.27 | -3.25 | 5.83 | -0.09 | -5.17 | -9.55 | -5.02 |
Cytoplasmic Foci | 8.22 | -3.02 | 0.45 | -5.14 | 3.04 | -4.18 | -14.94 | -8.01 | -3.24 | 12.38 | 2.06 | -14.23 | -3.14 | -5.14 | 12.82 |
Eisosomes | 5.62 | 1.30 | 0.35 | -6.10 | -1.03 | -0.03 | -7.41 | -13.47 | -14.63 | -0.36 | 4.48 | -4.57 | -6.38 | -15.58 | -0.26 |
Endoplasmic Reticulum | 3.90 | -3.12 | -2.07 | -4.62 | 2.60 | 1.24 | -4.08 | -5.18 | -5.83 | 1.00 | 2.71 | -4.99 | -5.05 | -6.16 | 2.47 |
Endosome | 3.99 | -5.66 | -2.11 | -4.64 | 3.70 | -4.31 | -11.26 | -22.55 | -18.09 | -1.69 | -1.77 | -12.58 | -20.78 | -19.47 | -0.27 |
Golgi | 3.50 | -2.03 | -1.51 | -3.43 | 0.55 | -6.34 | -12.15 | -2.13 | 4.72 | 11.65 | -3.55 | -11.66 | -0.58 | 2.80 | 11.40 |
Lipid Particles | 3.87 | 1.18 | 4.32 | 2.35 | 3.17 | -0.22 | 1.80 | -0.97 | -0.41 | -3.39 | 2.56 | 1.36 | 1.85 | -1.21 | 0.17 |
Mitochondria | -14.18 | 8.89 | 8.34 | 17.22 | -1.26 | 5.70 | 30.37 | 46.93* | 37.92* | -6.23 | -2.85 | 29.73 | 40.92* | 44.22* | -5.67 |
Mitotic Spindle | 0.74 | -6.48 | -7.66 | -7.78 | 0.86 | -1.84 | -9.95 | -13.29 | -12.52 | 6.45 | -1.54 | -11.88 | -14.89 | -14.31 | 6.90 |
None | 4.17 | 4.19 | 4.09 | -1.77 | -2.24 | -0.24 | 0.37 | -4.09 | -4.05 | -4.11 | 4.08 | 4.16 | 0.03 | -4.04 | -4.11 |
Nuclear Periphery | 0.65 | -2.06 | -0.44 | -1.19 | 1.96 | -0.70 | -4.11 | -3.97 | -3.86 | -2.82 | 0.17 | -2.31 | -3.38 | -3.68 | 1.31 |
Nuclear Periphery Foci | -0.87 | -2.26 | -1.83 | -1.68 | 1.66 | -0.48 | -2.27 | -7.34 | -6.44 | 0.54 | -1.20 | -2.70 | -5.87 | -4.76 | 1.99 |
Nucleolus | 3.21 | -0.92 | 1.88 | -3.25 | 1.99 | -0.84 | -1.60 | -7.08 | -6.14 | -6.81 | 2.20 | -1.44 | -5.62 | -6.84 | -3.04 |
Nucleus | 5.08 | -1.01 | 1.89 | -3.37 | 1.63 | -0.37 | -2.96 | -8.79 | -8.70 | -7.09 | 4.81 | -1.78 | -7.02 | -9.07 | -2.08 |
Peroxisomes | 3.91 | -3.64 | -2.51 | -4.19 | 1.54 | -0.25 | -5.89 | -5.29 | -4.59 | 4.44 | 1.45 | -7.03 | -4.19 | -5.35 | 5.58 |
Vacuole | 5.45 | -3.54 | -5.30 | -7.27 | -2.03 | -2.98 | -7.94 | -31.28 | -29.41 | -13.47 | 0.83 | -8.93 | -30.53 | -30.86 | -15.07 |
Vacuole Periphery | 6.45 | -0.53 | -1.77 | -5.51 | -1.13 | -1.15 | -5.24 | -19.54 | -18.72 | -5.05 | 3.47 | -5.17 | -18.13 | -21.22 | -6.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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