Standard name
Human Ortholog
Description Mitochondrial protein of unknown function involved in lipid homeostasis; associates with mitochondrial ribosome; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0.12 0.13 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0.05 0.05 0.05 0 0 0 0.06 0.06 0.08 0.05 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0.08 0.08 0.06 0 0 0 0 0.05 0.08 0.13 0.07 0.09 0.12 0 0 0 0.05 0 0
Golgi 0 0.07 0.41 0.18 0.26 0 0 0 0 0 0 0 0.34 0.41 0.33 0.4 0.48 0.22 0 0.09 0 0.1 0.1 0.12
Mitochondria 0.95 0.85 0.51 0.79 0.6 0.75 0.73 0.72 0.84 0.83 0.92 0.84 0.68 0.58 0.48 0.1 0.09 0.13 0.84 0.77 0.81 0.65 0.68 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.06 0 0.06 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0.09 0 0 0 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.18 0.33 0.08 0.17 0.17 0.17 0.11 0.1 0.11 0.05 0.08 0.24 0.15 0.17 0.29 0.17 0.25 0 0 0 0.06 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 3 2 0 1 0 2 0 0 1 5 3 4 1 5 1 4 1 1 3 7
Bud 3 7 1 5 0 3 3 7 4 5 8 10 1 3 3 6 6 10 2 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Bud Site 1 0 0 0 1 0 2 3 3 1 2 0 0 0 0 1 1 2 – – – – – –
Cell Periphery 4 17 4 10 4 2 0 1 0 5 5 1 14 7 7 18 16 12 0 1 0 0 0 0
Cytoplasm 2 8 3 9 12 14 18 26 16 9 13 14 13 16 25 8 6 10 0 1 0 5 0 1
Endoplasmic Reticulum 3 3 0 1 3 3 1 1 3 1 8 1 4 8 3 6 2 7 0 1 0 0 1 3
Endosome 2 8 3 8 14 26 28 32 9 13 0 11 11 21 41 11 11 16 11 10 6 8 7 9
Golgi 3 22 46 40 51 4 4 10 5 7 4 3 75 113 108 59 59 31 9 28 6 16 23 37
Mitochondria 280 253 57 174 119 253 243 356 277 268 357 311 150 160 156 14 11 18 250 244 129 109 158 207
Nucleus 1 1 2 2 1 5 14 15 8 7 6 8 1 1 1 4 4 4 0 1 2 1 2 1
Nuclear Periphery 17 10 1 1 4 20 11 32 22 10 23 12 0 2 1 3 2 1 4 2 0 2 1 2
Nucleolus 16 4 0 2 4 18 17 29 15 13 10 16 2 2 2 1 1 3 0 0 0 1 2 1
Peroxisomes 1 10 4 7 1 4 7 8 2 5 0 3 3 6 27 8 6 12 7 4 5 9 9 9
SpindlePole 1 3 3 4 7 6 9 21 5 8 3 15 1 5 7 7 2 17 1 0 0 0 0 1
Vac/Vac Membrane 29 53 37 18 33 56 57 56 33 35 19 31 54 42 57 42 21 34 8 13 3 10 16 28
Unique Cell Count 294 299 111 219 199 337 335 497 328 321 386 370 221 277 326 147 122 138 299 319 160 170 232 320
Labelled Cell Count 363 399 162 283 257 416 414 598 402 389 458 436 330 391 441 192 149 182 299 319 160 170 232 320


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.5 8.4 8.0 8.4 7.2 7.2 7.4 6.3 6.6 5.9 6.2 8.4 8.4 8.1 8.4 8.3 7.5 8.1 8.4 8.9
Std Deviation (1e-4) 0.8 1.2 1.3 1.7 1.6 1.5 1.8 1.7 1.4 1.5 1.5 1.5 1.1 1.2 1.3 1.8 1.8 1.3 1.9 1.4 1.5
Intensity Change (Log2) – – – -0.07 -0.0 -0.22 -0.22 -0.19 -0.42 -0.34 -0.51 -0.43 0.0 0.0 -0.05 0.01 -0.01 -0.15 -0.05 0.0 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.0 0 0 2.5 2.6 1.6
Cytoplasm 0 1.3 0 1.2 1.1 0 0 0 0 1.3 1.3 1.8 0 0 1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.6 1.9 2.0 1.5 0 0 0 0 0 1.8 3.0 1.7 2.0 2.6
Golgi -4.5 -2.9 -11.7 -11.6 -13.0 -11.3 -10.9 -12.5 -12.4 -1.3 -0.1 -1.6 -0.2 1.1 -3.2
Mitochondria 5.3 1.4 4.7 4.1 4.1 7.1 6.8 10.2 7.1 2.9 1.1 -0.6 -7.4 -7.1 -6.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.2 0 2.3 2.4 0 2.2 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1.7 0.7 0 1.6
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8
Vacuole -5.8 -3.4 -3.8 -3.7 -5.8 -5.8 -5.5 -8.3 -6.6 -1.7 -4.0 -3.5 -0.8 -2.8 -1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7267 2.8116 3.0177 3.0051 2.5713 3.0606 2.7518 2.6295 2.3566 2.1649 3.2916 2.9821 2.7761 2.8519 2.9088 2.8119 2.3325 2.5124
Actin 0.0017 0.0029 0.0006 0.0007 0.0004 0.0006 0.0149 0.0104 0.018 0.0082 0.0708 0.0249 0.0127 0.0171 0.0049 0.0114 0.0023 0.0106
Bud 0.0003 0.0006 0.004 0.0003 0.0002 0.0001 0.0008 0.0028 0.0006 0.0008 0.0019 0.0024 0.0013 0.0019 0.0017 0.0005 0.0014 0.0007
Bud Neck 0.0003 0.0002 0.0001 0.0001 0 0.0004 0.0021 0.0014 0.0011 0.0007 0.0028 0.0014 0.0011 0.0018 0.0011 0.0005 0.0002 0.0008
Bud Periphery 0.0014 0.0014 0.0015 0.001 0.0006 0.0005 0.0016 0.0059 0.0009 0.0028 0.0041 0.0078 0.0021 0.0028 0.0028 0.0011 0.0026 0.0026
Bud Site 0.0005 0.0013 0.0004 0.0002 0.0002 0.0001 0.0058 0.019 0.0055 0.0069 0.0138 0.0015 0.0043 0.0312 0.0168 0.0017 0.0011 0.0009
Cell Periphery 0.0004 0.0006 0.0002 0.0002 0.0002 0.0002 0.0012 0.0065 0.0006 0.0005 0.0012 0.0005 0.0008 0.0022 0.0011 0.0002 0.0002 0.0012
Cytoplasm 0.001 0.001 0.001 0.0003 0.0001 0.0003 0.0047 0.0048 0.0149 0.0004 0.0048 0.0141 0.0114 0.0056 0.0089 0.0011 0.0066 0.0007
Cytoplasmic Foci 0.0097 0.006 0.0037 0.0055 0.0034 0.0025 0.0205 0.0294 0.0202 0.0051 0.0228 0.0134 0.0333 0.0276 0.0131 0.0122 0.0151 0.003
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0006 0.0022 0.0003 0.0003 0.0007 0.0003 0.0015 0.0003 0.0003 0.0002 0.0001 0.0006
Endoplasmic Reticulum 0.0012 0.0008 0.001 0.0001 0.0001 0.0003 0.0145 0.0027 0.0012 0.0006 0.0047 0.0013 0.0052 0.0014 0.0036 0.0003 0.002 0.0008
Endosome 0.0478 0.0418 0.0168 0.0216 0.0136 0.0156 0.0449 0.1049 0.0314 0.0323 0.1464 0.0419 0.0701 0.0639 0.0319 0.0216 0.0468 0.0285
Golgi 0.0316 0.0355 0.0143 0.0192 0.0223 0.0185 0.0334 0.0655 0.0264 0.0106 0.0443 0.0484 0.0414 0.0405 0.0221 0.0317 0.0126 0.0393
Lipid Particles 0.0227 0.0175 0.0067 0.0011 0.001 0.0123 0.0435 0.0157 0.017 0.0293 0.0203 0.0179 0.0382 0.0145 0.0056 0.0119 0.012 0.005
Mitochondria 0.8306 0.8608 0.9316 0.9103 0.9407 0.9303 0.7449 0.6521 0.8282 0.8602 0.5329 0.7827 0.7234 0.7326 0.8496 0.8825 0.8458 0.8879
None 0.0007 0.0003 0.0004 0.0001 0.0001 0.0006 0.015 0.0029 0.0004 0.0004 0.0031 0.0022 0.0045 0.0039 0.0011 0.0009 0.009 0.0003
Nuclear Periphery 0.0017 0.0005 0.0006 0 0 0.0003 0.0121 0.002 0.0002 0.0008 0.0459 0.0107 0.0126 0.0015 0.0009 0.0001 0.004 0.0014
Nucleolus 0.0026 0.0006 0.0004 0 0.0001 0.0004 0.002 0.0022 0.0033 0.001 0.0013 0.0006 0.0019 0.0027 0.0007 0.0001 0.0007 0.0002
Nucleus 0.0007 0.0004 0.001 0 0.0001 0.0004 0.0058 0.0015 0.0006 0.0007 0.02 0.0016 0.0019 0.0015 0.0006 0.0001 0.0013 0.0003
Peroxisomes 0.024 0.007 0.0103 0.0358 0.0138 0.0105 0.012 0.0294 0.0216 0.0049 0.0339 0.0162 0.0154 0.0139 0.0108 0.0166 0.0162 0.0032
Punctate Nuclear 0.0004 0.0001 0.0001 0 0 0.0002 0.005 0.0003 0.0006 0.0005 0.005 0.0008 0.0014 0.0005 0.0003 0.0002 0.0091 0.0002
Vacuole 0.009 0.0109 0.0019 0.0011 0.0011 0.0021 0.0089 0.0265 0.0033 0.0122 0.0086 0.0033 0.0091 0.0216 0.0117 0.0021 0.0067 0.0059
Vacuole Periphery 0.0118 0.0097 0.0035 0.0022 0.002 0.0038 0.0059 0.0119 0.0034 0.0207 0.0106 0.0061 0.0064 0.011 0.0102 0.0029 0.0041 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.26 10.3829 15.8372 15.0146 12.9675 18.0574 16.9137 18.5011 19.7258 19.6228
Translational Efficiency 0.5618 0.7062 0.4371 0.6015 0.4919 0.431 0.6505 0.4234 0.4488 0.4223

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial protein of unknown function involved in lipid homeostasis; associates with mitochondrial ribosome; integral membrane protein that localizes to the mitochondrial inner membrane; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of the ERMES complex; transcription is periodic during the metabolic cycle; homologous to human aarF domain containing kinase, ADCK1
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mcp2

Mcp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available