Standard name
Human Ortholog
Description Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0.08 0.1 0.05 0.05 0.06 0 0 0.06 0.12 0.14 0.15 0.07 0 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.33 0 0.1 0.29 0.18 0.28 0.31 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.89 0.87 0.89 0.85 0.79 0.76 0.88 0.86 0.76 0.8 0.87 0.79 0.84 0.79 0.77 0.79 0.74 0.71 0.86 0.85 0.8 0.8 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0 0.06 0 0.09 0.09 0.05 0 0 0.07 0 0.05 0 0 0.07 0.06 0.11 0.16 0.17 0.06 0.1 0.12 0.13 0.14 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 2
Bud 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 1 0 0 4 4 0 6 9 6
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 4
Bud Site 0 1 0 0 0 0 0 2 4 3 8 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 17 20 9 10 10 18 12 14 10 11 4 11 87 127 143 19 11 26 2 2 0 6 5 4
Endoplasmic Reticulum 0 3 1 0 0 0 0 0 0 0 0 0 3 0 1 11 6 9 1 0 0 0 1 0
Endosome 7 0 1 2 4 0 7 9 0 1 0 0 7 15 26 6 10 12 7 4 3 2 32 36
Golgi 2 0 0 0 0 0 0 1 0 0 0 0 1 1 0 2 1 1 7 1 0 0 1 2
Mitochondria 15 61 0 1 1 62 8 29 47 45 31 53 5 2 1 15 3 9 6 3 1 2 9 9
Nucleus 441 517 110 129 99 142 193 241 123 205 96 136 630 696 729 223 189 197 446 532 109 446 625 488
Nuclear Periphery 4 0 1 1 1 0 2 1 3 2 1 0 0 0 1 3 3 1 0 0 0 1 5 5
Nucleolus 2 1 0 0 1 4 2 8 7 11 9 6 1 3 3 5 7 10 2 1 3 11 31 40
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 1 6 0 0 0 0 0 0 0 1 0
SpindlePole 0 4 2 1 1 0 0 3 0 3 0 1 2 2 8 5 7 6 7 7 3 5 28 44
Vac/Vac Membrane 21 33 4 13 11 9 4 9 11 11 5 4 32 60 59 31 42 46 30 60 16 70 126 137
Unique Cell Count 494 592 123 152 125 187 219 281 162 255 110 173 749 879 947 281 257 277 522 626 138 556 895 798
Labelled Cell Count 509 640 128 157 128 238 228 318 207 293 154 214 768 909 977 322 279 317 522 626 138 556 895 798


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.1 6.3 6.4 7.8 4.3 6.0 5.6 5.8 5.4 5.8 5.1 6.2 6.1 6.4 7.4 8.9 8.3 5.3 5.8 6.1
Std Deviation (1e-4) 1.1 0.7 1.1 1.7 8.1 1.3 1.6 1.0 6.0 1.2 6.0 1.2 1.1 1.0 1.3 1.7 1.5 1.6 1.0 1.3 1.3
Intensity Change (Log2) – – – 0.02 0.31 -0.56 -0.08 -0.17 -0.12 -0.21 -0.13 -0.29 -0.02 -0.04 0.02 0.23 0.5 0.41 -0.24 -0.12 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.2 0.7 -0.7 -0.9 -0.4 -1.2 -1.2 -0.3 1.4 2.2 2.3 -0.2 -1.2 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.7 6.5 5.0 6.3 6.8 0 0 0 0 0 0
Nucleus -1.1 -2.2 -3.0 -0.4 -1.0 -2.9 -2.2 -0.5 -2.4 -1.5 -2.7 -3.2 -2.4 -3.5 -4.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 1.8 0.7 0 0 1.3 0 0 0 0.5 1.5 1.3 2.6 3.7 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0351 1.932 1.7809 1.6949 1.0817 1.7702 4.0943 3.5195 4.1455 3.5495 3.749 3.6164 5.7938 5.4456 5.2855 5.3248 5.4041 5.2871
Actin 0 0 0 0 0 0 0 0 0 0 0 0 0.0141 0.0001 0.0004 0.0258 0.0112 0.0003
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0.0029 0.0016 0.0004 0.0036 0.0272 0.0006
Bud Neck 0 0.0001 0 0.0001 0.0002 0.0003 0 0.0001 0 0 0.0001 0.0004 0.0048 0.0002 0.0008 0.0018 0.0008 0.0012
Bud Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.002 0.0002 0.0015 0.0098 0.0059 0.0006
Bud Site 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0.0035 0.0008 0.0015 0.0179 0.0033 0.0002
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0006 0 0.0023 0.0013 0.0005 0.0001
Cytoplasm 0.0001 0.0049 0.0019 0.0003 0.0003 0.0002 0.0005 0.0046 0.0002 0.0003 0.0008 0.0002 0.0174 0.0079 0.0069 0.0065 0.0027 0.0051
Cytoplasmic Foci 0 0.0001 0 0 0 0 0 0 0 0 0 0 0.0081 0.0006 0.003 0.0066 0.0052 0.0044
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0.0002 0.0003 0
Endoplasmic Reticulum 0 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0.0056 0.0002 0.0003 0.0029 0.0019 0.0001
Endosome 0 0.0002 0.0001 0 0 0 0.0005 0.0004 0 0 0 0 0.0127 0.0002 0.0012 0.0122 0.0172 0.0003
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.0036 0 0.0001 0.009 0.0073 0.0001
Lipid Particles 0 0 0 0 0 0 0 0 0 0 0 0 0.0147 0 0.0002 0.0021 0.0265 0.0005
Mitochondria 0 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0.0001 0 0.0068 0.0065 0.0022 0.0157 0.0532 0.0029
None 0 0.0007 0.002 0 0.0002 0.0001 0.0001 0.0029 0 0.0001 0.0002 0 0.0071 0.0088 0.002 0.0019 0.0023 0.0023
Nuclear Periphery 0.0016 0.0049 0.0027 0.0019 0.0024 0.001 0.0008 0.0079 0.0013 0.0005 0.0028 0.0006 0.0269 0.0039 0.0037 0.0419 0.0053 0.002
Nucleolus 0.0027 0.0053 0.0035 0.007 0.0116 0.0076 0.0028 0.0052 0.0037 0.0021 0.0086 0.0091 0.024 0.0068 0.0142 0.0134 0.0084 0.0143
Nucleus 0.9945 0.9769 0.9885 0.9881 0.9722 0.9836 0.9917 0.9708 0.9881 0.9962 0.9839 0.9813 0.7962 0.9464 0.9395 0.8116 0.7731 0.9085
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0038 0 0.0001 0.0029 0.0093 0.0004
Punctate Nuclear 0.001 0.0061 0.0013 0.0024 0.0128 0.0073 0.0024 0.0063 0.0066 0.0007 0.0034 0.0082 0.0364 0.0152 0.0161 0.0024 0.034 0.0547
Vacuole 0 0.0003 0.0001 0 0 0 0.0009 0.001 0 0 0.0001 0 0.0054 0.0004 0.0029 0.0064 0.0027 0.0009
Vacuole Periphery 0 0.0001 0 0 0 0 0.0001 0.0003 0 0 0 0 0.0029 0.0001 0.0006 0.0042 0.0018 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
130 762 164 136 896 1343 1704 1547 1026 2105 1868 1683

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.04 908.86 930.86 991.79 726.87 819.63 863.56 897.33 723.72 851.93 869.47 904.96
Standard Deviation 89.31 132.14 111.06 173.71 100.56 119.19 124.54 142.57 99.54 131.24 124.88 147.60
Intensity Change Log 2 — 0.372505 0.407011 0.498481 — 0.173275 0.248599 0.303941 — 0.274597 0.328603 0.402799

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000103 0.000692 0.000781 0.001169 0.000080 0.000299 0.000484 0.000878 0.000083 0.000441 0.000510 0.000902
Bud Neck 0.001227 0.010816 0.003698 0.005919 0.001880 0.010837 0.014316 0.030997 0.001797 0.010829 0.013384 0.028970
Bud Site 0.000311 0.002997 0.001059 0.004011 0.000278 0.004190 0.003156 0.012906 0.000282 0.003758 0.002971 0.012187
Cell Periphery 0.000065 0.000216 0.000086 0.000176 0.000098 0.000197 0.000120 0.000227 0.000094 0.000204 0.000117 0.000223
Cytoplasm 0.012635 0.030119 0.004339 0.033898 0.017408 0.056255 0.010318 0.058430 0.016804 0.046794 0.009793 0.056447
Cytoplasmic Foci 0.004965 0.003281 0.000149 0.006390 0.001711 0.007839 0.000550 0.005941 0.002123 0.006189 0.000514 0.005978
Eisosomes 0.000019 0.000029 0.000035 0.000083 0.000012 0.000026 0.000020 0.000035 0.000013 0.000027 0.000021 0.000039
Endoplasmic Reticulum 0.002093 0.011091 0.007961 0.013152 0.002042 0.004954 0.005877 0.006600 0.002048 0.007176 0.006060 0.007130
Endosome 0.000328 0.002501 0.000381 0.004085 0.000415 0.002482 0.000614 0.006897 0.000404 0.002489 0.000594 0.006670
Golgi 0.000034 0.000847 0.000056 0.000247 0.000226 0.001392 0.000255 0.003411 0.000202 0.001195 0.000237 0.003155
Lipid Particles 0.000543 0.001171 0.000295 0.001765 0.000530 0.002292 0.000173 0.002778 0.000532 0.001886 0.000183 0.002696
Mitochondria 0.000339 0.002565 0.000684 0.001731 0.001031 0.004699 0.001662 0.008571 0.000943 0.003926 0.001576 0.008018
Mitotic Spindle 0.000008 0.005432 0.000184 0.027168 0.001232 0.009329 0.004413 0.029712 0.001077 0.007918 0.004042 0.029506
None 0.005048 0.010160 0.001923 0.007187 0.009801 0.011532 0.005586 0.010171 0.009198 0.011035 0.005265 0.009930
Nuclear Periphery 0.000256 0.002832 0.000535 0.001914 0.000509 0.002301 0.001124 0.004639 0.000477 0.002493 0.001072 0.004419
Nuclear Periphery Foci 0.000063 0.001168 0.000216 0.002940 0.000296 0.000824 0.000244 0.004041 0.000266 0.000949 0.000241 0.003952
Nucleolus 0.002852 0.007945 0.002796 0.010293 0.003682 0.006327 0.002990 0.013591 0.003577 0.006912 0.002973 0.013325
Nucleus 0.968116* 0.896985* 0.971222* 0.839649* 0.957113* 0.863492* 0.944187* 0.774313* 0.958507* 0.875617* 0.946561* 0.779593*
Peroxisomes 0.000331 0.001100 0.000179 0.003272 0.000951 0.002243 0.000100 0.001599 0.000873 0.001829 0.000107 0.001734
Vacuole 0.000613 0.006833 0.003322 0.033105 0.000591 0.007569 0.003567 0.017532 0.000594 0.007303 0.003546 0.018790
Vacuole Periphery 0.000052 0.001222 0.000100 0.001843 0.000113 0.000920 0.000245 0.006733 0.000105 0.001029 0.000232 0.006338

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.22 -7.44 -8.01 -5.32 -3.91 -8.85 -17.77 -12.28 -8.47 -4.77 -11.85 -19.17 -13.99 -7.79 -5.65
Bud Neck -8.75 -4.39 -5.07 -0.15 -2.91 -9.35 -12.32 -17.52 -12.66 -9.55 -12.74 -12.63 -17.81 -12.77 -9.75
Bud Site -4.43 -3.74 -3.74 -2.57 -3.23 -5.74 -5.27 -9.21 -5.50 -5.79 -7.19 -5.44 -9.71 -6.32 -6.24
Cell Periphery -4.40 -1.18 -4.68 -0.02 -3.06 -3.63 -1.04 -3.58 0.82 -3.47 -4.76 -1.32 -4.34 1.09 -4.02
Cytoplasm -3.54 1.93 -1.42 1.09 -3.00 -8.94 2.42 -8.15 2.07 -12.68 -9.01 2.69 -8.45 0.57 -13.02
Cytoplasmic Foci 0.49 1.36 -0.19 -0.97 -2.22 -4.68 2.25 -2.36 2.82 -4.29 -3.90 2.56 -1.97 1.99 -4.73
Eisosomes -2.98 -3.64 -8.23 -7.36 -5.08 -8.30 -8.53 -13.26 -5.11 -8.25 -9.82 -8.99 -14.98 -7.32 -9.35
Endoplasmic Reticulum -12.47 -8.31 -8.83 -3.37 -4.50 -8.73 -13.83 -12.24 -4.03 -1.19 -14.98 -15.47 -14.39 -1.26 -2.54
Endosome -3.18 -1.02 -3.09 -1.32 -2.89 -5.23 -1.09 -6.44 -2.90 -6.28 -5.75 -1.25 -6.92 -3.11 -6.70
Golgi -1.51 -2.57 -3.74 0.60 -3.24 -2.23 0.05 -2.40 -0.65 -2.44 -2.46 -0.01 -2.48 -0.87 -2.49
Lipid Particles -2.54 0.38 -3.68 -2.17 -3.89 -3.33 1.98 -1.80 0.80 -2.40 -3.75 2.08 -2.02 0.27 -2.62
Mitochondria -5.14 -5.15 -2.61 0.31 -1.99 -4.17 -1.60 -5.40 -1.76 -5.12 -4.54 -1.80 -5.65 -2.51 -5.26
Mitotic Spindle -3.33 -2.32 -3.20 -2.59 -3.18 -4.18 -3.47 -9.76 -6.53 -7.82 -4.93 -3.55 -10.30 -7.46 -8.42
None -1.51 1.16 0.01 1.70 -1.38 -0.66 2.26 0.74 2.05 -2.83 -0.78 2.35 0.64 2.13 -3.05
Nuclear Periphery -8.53 -5.29 -6.68 0.20 -5.51 -8.65 -9.38 -8.79 -4.90 -6.96 -11.63 -9.95 -9.23 -4.62 -7.31
Nuclear Periphery Foci -3.51 -1.46 -2.63 -2.43 -2.56 -3.06 -0.37 -4.71 -3.93 -4.64 -4.01 -0.71 -5.33 -4.37 -5.21
Nucleolus -4.04 -1.10 -2.70 -1.65 -1.77 -3.11 -0.12 -9.08 -7.62 -9.09 -4.55 -0.67 -9.50 -7.44 -9.20
Nucleus 6.96 0.79 7.03 4.19 6.94 12.20 4.17 21.69 9.28 20.06 13.86 4.37 22.97 11.74 21.21
Peroxisomes -2.17 0.64 -1.57 -1.28 -1.60 -1.70 1.59 -0.62 1.43 -3.78 -1.65 1.62 -1.23 0.55 -3.99
Vacuole -5.87 -4.62 -6.67 -5.42 -5.92 -8.11 -7.84 -12.65 -6.31 -9.68 -10.02 -8.56 -14.10 -8.02 -11.05
Vacuole Periphery -1.64 -3.68 -1.55 -0.04 -1.45 -5.82 -2.05 -3.60 -2.77 -3.48 -3.44 -2.28 -3.70 -2.61 -3.57
Standard name
Human Ortholog
Description Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ndl1

Ndl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ndl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available