Standard name
Human Ortholog
Description Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.11 0.11 0.07 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.46 0.8 0.82 0.91 0.91 0.92 0.93 0.85 – 0.79 0.85 0.46 0.57 0.6 0.58 0.63 0.63 0.29 0.27 0.39 0.35 0.29 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0.08 0.1 0.16 0.1 0.1 0.09 0.07 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.3 0 0 0 0.09 0.1 0.06 0.26 – 0.3 0.17 0.19 0.05 0 0.06 0.05 0.06 0.12 0.09 0.15 0.12 0.2 0.16
Nucleus 0.12 0.11 0.06 0 0 0 0 0 0 – 0 0 0.12 0.09 0.06 0.06 0.08 0.07 0.11 0.14 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.1 0.06 0.1 0.06 0.06
Vac/Vac Membrane 0.08 0.23 0 0 0 0 0 0 0 – 0 0 0.21 0.26 0.24 0.3 0.21 0.23 0.18 0.23 0.19 0.24 0.28 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0 3 2 4 1 6 6
Bud 0 1 0 2 3 2 3 2 6 – 7 6 1 1 0 2 0 3 3 3 2 1 8 11
Bud Neck 2 0 39 34 37 18 42 29 16 – 7 20 0 0 0 5 2 6 1 2 3 1 4 12
Bud Site 0 1 1 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 0 1 1 3 0 3 – 4 1 0 0 1 0 0 3 0 0 1 0 1 2
Cytoplasm 85 66 275 262 479 503 767 638 415 – 348 504 112 184 282 427 369 354 88 59 172 58 143 160
Endoplasmic Reticulum 6 0 7 2 1 1 2 0 0 – 0 0 2 3 0 62 38 46 5 0 4 3 7 12
Endosome 1 5 0 1 2 0 1 0 0 – 0 1 19 31 74 75 61 50 20 15 9 6 19 12
Golgi 0 0 1 0 0 0 0 1 0 – 0 0 0 1 0 0 1 2 0 1 0 1 2 3
Mitochondria 74 43 2 13 6 50 81 39 128 – 131 101 46 16 17 44 27 33 35 19 66 20 98 77
Nucleus 22 16 19 10 4 2 4 11 5 – 7 4 30 30 29 41 46 40 32 31 22 6 12 11
Nuclear Periphery 0 1 0 0 1 0 1 1 1 – 0 0 0 0 0 1 0 0 1 1 1 0 0 1
Nucleolus 0 0 0 0 1 0 0 2 2 – 1 2 1 1 0 1 0 0 3 2 1 0 2 2
Peroxisomes 0 0 0 0 1 0 0 0 0 – 0 0 0 0 2 0 0 0 1 0 0 0 1 1
SpindlePole 5 1 6 13 6 10 7 10 6 – 10 15 4 3 5 13 13 9 31 23 25 16 30 30
Vac/Vac Membrane 15 33 1 3 11 8 2 1 1 – 0 2 52 85 113 225 124 131 55 51 83 40 138 117
Unique Cell Count 185 143 343 318 526 554 831 685 486 443 595 246 322 472 738 587 562 303 223 445 168 496 482
Labelled Cell Count 211 167 358 342 554 595 913 734 583 516 656 267 355 523 896 681 677 303 223 445 168 496 482


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.8 4.5 4.2 4.8 3.5 3.6 4.1 3.2 – 3.2 3.5 4.0 4.2 4.2 5.8 5.8 5.6 3.9 3.8 4.3
Std Deviation (1e-4) 0.5 0.6 0.5 1.0 1.1 1.0 0.9 1.1 1.0 – 1.5 0.8 0.8 1.1 0.9 1.0 1.1 1.1 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.12 0.08 -0.36 -0.32 -0.15 -0.5 – -0.5 -0.38 -0.19 -0.1 -0.12 0.35 0.36 0.31 -0.23 -0.26 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 11.4 9.0 9.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5875 -1.3428 -1.5177 -1.8163 -1.6851 -1.5394 -0.9066 -0.6649 -0.7549 -0.8861 -1.0856 -0.8478 -1.5163 -1.2558 -1.2799 -0.7809 -1.1643 -1.2989
Actin 0.0104 0.0003 0.011 0.0006 0.0193 0.0039 0.0141 0.0003 0.0118 0.0011 0.0034 0.0056 0.0289 0.0002 0.0025 0.0203 0.0289 0.0043
Bud 0.0036 0 0.005 0 0.0002 0.0025 0.0006 0.0005 0.0006 0.0001 0.0002 0.0008 0.0002 0.0008 0.0022 0.0014 0.0007 0.0001
Bud Neck 0.0028 0.0003 0.0011 0.0015 0.0013 0.0032 0.0017 0.0009 0.0005 0.0012 0.0016 0.0049 0.0013 0.0003 0.0005 0.0008 0.0015 0.0016
Bud Periphery 0.0029 0 0.0017 0 0.0005 0.0013 0.0008 0.0006 0.0015 0.0002 0.0003 0.0031 0.0004 0.0005 0.0033 0.0017 0.0012 0.0001
Bud Site 0.006 0.0005 0.0055 0.0003 0.0003 0.0002 0.0028 0.0036 0.0019 0.0005 0.001 0.0005 0.0018 0.0024 0.0052 0.0067 0.0026 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.1194 0.1582 0.1226 0.1659 0.1025 0.1858 0.123 0.1553 0.1554 0.1818 0.2084 0.2055 0.1338 0.1826 0.118 0.1454 0.2003 0.2097
Cytoplasmic Foci 0.0468 0.0149 0.0169 0.0161 0.0217 0.035 0.0454 0.0236 0.0194 0.057 0.0303 0.0294 0.0255 0.018 0.0165 0.019 0.0119 0.0138
Eisosomes 0.0005 0.0001 0.0004 0.0003 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0069 0.0057 0.0042 0.0044 0.0023 0.0035 0.007 0.0065 0.0036 0.0094 0.0029 0.0032 0.0044 0.0041 0.0029 0.0022 0.0045 0.0032
Endosome 0.0244 0.0102 0.0113 0.0106 0.0117 0.0097 0.0286 0.0088 0.014 0.0122 0.0191 0.014 0.016 0.0076 0.0108 0.0073 0.0162 0.0094
Golgi 0.004 0.0003 0.0013 0.0003 0.0049 0.0014 0.0068 0.0007 0.0047 0.003 0.0038 0.0016 0.003 0.0002 0.0009 0.0026 0.0024 0.001
Lipid Particles 0.0194 0.0065 0.0046 0.0077 0.0065 0.0045 0.0105 0.0038 0.0024 0.0022 0.0086 0.0024 0.0069 0.0021 0.0043 0.0024 0.0032 0.0038
Mitochondria 0.0094 0.0004 0.007 0.0008 0.002 0.0009 0.0021 0.0008 0.0064 0.0013 0.0236 0.001 0.0015 0.0006 0.0015 0.0021 0.0019 0.0008
None 0.5197 0.6247 0.5655 0.4877 0.5667 0.4702 0.5831 0.6504 0.6117 0.5065 0.5278 0.4878 0.5726 0.6219 0.6235 0.5235 0.4451 0.5372
Nuclear Periphery 0.0229 0.0187 0.0144 0.0186 0.0245 0.0143 0.0114 0.011 0.0118 0.0236 0.0087 0.0087 0.0148 0.0267 0.0156 0.0136 0.0195 0.0132
Nucleolus 0.0073 0.0035 0.0064 0.0056 0.0057 0.0059 0.0036 0.0031 0.0023 0.0022 0.0028 0.0051 0.0028 0.0037 0.0038 0.0041 0.0053 0.0037
Nucleus 0.1118 0.1098 0.1456 0.1625 0.1302 0.1674 0.0811 0.1044 0.094 0.1016 0.0991 0.1434 0.1345 0.0918 0.1413 0.1618 0.1914 0.1304
Peroxisomes 0.0097 0.0004 0.0074 0.0013 0.0009 0.001 0.0117 0.0008 0.0118 0.0122 0.0019 0.0037 0.0018 0.0004 0.0023 0.003 0.0018 0.0008
Punctate Nuclear 0.0666 0.0418 0.0647 0.1128 0.0968 0.0871 0.0608 0.0204 0.0423 0.0804 0.0515 0.0769 0.046 0.0325 0.0412 0.0801 0.0552 0.0649
Vacuole 0.004 0.0031 0.003 0.0024 0.001 0.0017 0.0036 0.0036 0.0028 0.0025 0.003 0.0019 0.0024 0.0026 0.0027 0.0012 0.0052 0.0013
Vacuole Periphery 0.0011 0.0005 0.0006 0.0003 0.0008 0.0004 0.0009 0.0006 0.0007 0.0009 0.0013 0.0004 0.0006 0.0005 0.0008 0.0004 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.5039 11.7091 12.5117 15.8158 8.8309 15.1857 16.5747 20.2961 17.9354 18.1057
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2109 1171 1152 1182 1219 1563 1210 251 3328 2734 2362 1433

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 632.64 713.02 894.72 809.24 718.42 743.68 813.51 825.23 664.06 730.55 853.12 812.04
Standard Deviation 65.27 99.45 117.57 106.74 80.71 89.46 102.38 95.05 82.42 95.09 117.30 104.97
Intensity Change Log 2 — 0.172558 0.500051 0.355183 — 0.049854 0.179333 0.199969 — 0.108612 0.338410 0.274731

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000037 0.000154 0.000487 0.000946 0.000168 0.000198 0.000107 0.000696 0.000085 0.000179 0.000293 0.000902
Bud Neck 0.011937 0.007092 0.007013 0.010093 0.001582 0.006967 0.004042 0.009230 0.008144 0.007020 0.005491 0.009942
Bud Site 0.001825 0.001732 0.003631 0.045607 0.001068 0.004733 0.001346 0.027559 0.001548 0.003448 0.002460 0.042445
Cell Periphery 0.000124 0.000133 0.000114 0.000212 0.000129 0.000149 0.000567 0.000229 0.000126 0.000142 0.000346 0.000215
Cytoplasm 0.435849* 0.297196* 0.081171 0.350656* 0.358863* 0.289621* 0.208098* 0.287905* 0.407650* 0.292866* 0.146193* 0.339665*
Cytoplasmic Foci 0.130528 0.085936 0.001818 0.023612 0.068404 0.135558 0.014096 0.018066 0.107773 0.114305 0.008108 0.022640
Eisosomes 0.000102 0.000066 0.000026 0.000055 0.000054 0.000098 0.000291 0.000079 0.000084 0.000084 0.000162 0.000060
Endoplasmic Reticulum 0.001001 0.001980 0.005752 0.002846 0.001155 0.001653 0.001719 0.003233 0.001058 0.001793 0.003686 0.002914
Endosome 0.004052 0.004541 0.001223 0.010171 0.002213 0.006778 0.001295 0.004317 0.003378 0.005820 0.001260 0.009146
Golgi 0.000884 0.000597 0.000087 0.006449 0.000472 0.002288 0.000061 0.000132 0.000733 0.001564 0.000074 0.005342
Lipid Particles 0.011729 0.010060 0.000719 0.001852 0.005537 0.017729 0.001755 0.002879 0.009461 0.014444 0.001250 0.002032
Mitochondria 0.003675 0.005826 0.000969 0.018549 0.000823 0.013695 0.000612 0.001278 0.002631 0.010325 0.000786 0.015524
Mitotic Spindle 0.000680 0.007208 0.010205 0.065534 0.002813 0.009698 0.005230 0.020325 0.001461 0.008632 0.007656 0.057615
None 0.006870 0.007679 0.004783 0.004253 0.008598 0.006378 0.018797 0.017559 0.007503 0.006935 0.011962 0.006584
Nuclear Periphery 0.001745 0.003235 0.002060 0.003541 0.001672 0.004016 0.001390 0.001961 0.001718 0.003682 0.001717 0.003264
Nuclear Periphery Foci 0.000491 0.001644 0.000838 0.001692 0.001909 0.001889 0.000461 0.001216 0.001011 0.001784 0.000645 0.001608
Nucleolus 0.002070 0.007323 0.001517 0.000657 0.003034 0.006346 0.002058 0.000977 0.002423 0.006765 0.001794 0.000713
Nucleus 0.367932* 0.536652* 0.867219* 0.344211* 0.531207* 0.466821* 0.721656* 0.500406* 0.427738* 0.496730* 0.792650* 0.371569*
Peroxisomes 0.002721 0.005163 0.000446 0.002366 0.002452 0.010128 0.000326 0.001330 0.002623 0.008001 0.000385 0.002185
Vacuole 0.015330 0.015011 0.009780 0.100269 0.007647 0.014020 0.015947 0.100353 0.012516 0.014444 0.012939 0.100284
Vacuole Periphery 0.000418 0.000770 0.000143 0.006429 0.000199 0.001237 0.000146 0.000267 0.000338 0.001037 0.000144 0.005350

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.91 -16.36 -5.68 -4.25 0.57 -0.50 -0.71 -2.37 -2.42 -2.36 -3.24 -10.09 -5.61 -4.53 -1.70
Bud Neck 3.75 1.71 2.99 -0.79 1.15 -9.55 -4.29 -2.85 0.46 -0.86 1.36 1.25 0.38 -0.83 -0.78
Bud Site 0.05 -2.26 -7.28 -7.31 -6.17 -3.32 -1.15 -2.58 -1.75 -2.45 -2.90 -2.36 -7.78 -6.93 -7.15
Cell Periphery -0.88 -0.37 -2.16 -1.26 -1.97 -1.34 -5.81 -0.72 0.01 5.26 -1.78 -5.77 -2.21 -0.84 4.67
Cytoplasm 15.57 54.57 13.79 -1.29 -26.75 7.24 17.36 7.94 3.79 -2.54 18.17 46.55 13.98 -0.39 -20.16
Cytoplasmic Foci 7.21 35.92 29.82 16.64 -11.65 -12.01 17.28 15.07 24.95 -0.30 -2.41 38.19 32.51 29.78 -7.46
Eisosomes 5.67 15.67 7.87 1.91 -9.40 -7.50 -2.26 -5.03 0.54 1.71 -0.99 -1.19 4.02 4.49 1.59
Endoplasmic Reticulum -5.57 -17.15 -12.02 -7.64 7.92 -3.19 -10.10 -8.59 -7.58 -3.57 -6.06 -19.30 -14.80 -11.29 3.71
Endosome -1.82 6.81 -1.88 -0.53 -5.72 -7.16 2.93 -1.24 4.57 -2.51 -5.94 7.36 -2.63 1.53 -5.98
Golgi 1.40 5.19 -1.87 -2.17 -2.80 -3.85 1.81 1.66 5.31 -1.91 -3.12 5.16 -1.89 -0.71 -2.83
Lipid Particles 0.58 14.91 13.44 10.09 -7.15 -9.73 8.65 5.99 12.79 -1.80 -6.18 16.59 15.21 16.74 -4.24
Mitochondria -2.17 4.28 -2.15 -0.98 -3.62 -8.00 1.16 0.05 7.93 -0.49 -7.29 4.55 -2.54 1.99 -3.73
Mitotic Spindle -4.09 -5.15 -8.75 -6.25 -4.50 -3.02 -2.65 -2.15 -0.79 -0.94 -5.47 -6.03 -8.82 -6.36 -5.48
None -1.50 2.99 3.55 5.18 0.96 2.35 -3.27 -0.03 -0.98 2.05 0.29 -2.17 3.74 3.48 4.84
Nuclear Periphery -4.58 -4.38 -4.18 -1.50 -2.48 -4.88 -4.00 -6.13 1.10 -3.37 -6.31 -5.45 -4.84 -0.72 -3.02
Nuclear Periphery Foci -5.49 -4.06 -5.60 0.79 -1.50 0.37 2.27 1.53 2.01 -1.87 -3.07 0.01 -1.91 1.37 -2.52
Nucleolus -6.09 -0.74 6.02 7.18 4.56 -4.37 1.65 6.33 8.11 3.26 -7.95 0.63 8.31 10.74 4.84
Nucleus -15.26 -60.45 -2.78 10.42 42.90 5.72 -16.98 2.28 -0.83 11.66 -8.31 -51.37 1.59 7.83 39.31
Peroxisomes -3.63 7.40 4.18 5.56 -2.01 -6.53 2.94 2.39 10.16 -0.94 -8.20 6.83 4.01 11.04 -2.50
Vacuole -0.77 -1.12 -23.62 -23.22 -22.82 -5.84 -10.53 -12.71 -12.33 -11.07 -2.67 -8.25 -27.03 -26.55 -24.40
Vacuole Periphery -1.88 4.31 -3.26 -2.70 -3.65 -3.26 2.57 0.10 3.25 -1.19 -3.47 4.78 -3.34 -2.03 -3.67
Standard name
Human Ortholog
Description Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (34%), cytoplasm (6%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdr8

Pdr8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available