Standard name | |
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Human Ortholog | |
Description | Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 12 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 2 | 11 | 3 | 4 | 6 | 2 | 1 | 6 | 1 | 0 | 0 | 12 | 1 | 6 | 0 | 0 | 0 | 1 | 3 | 0 |
Cytoplasm | 180 | 187 | 49 | 145 | 237 | 231 | 224 | 197 | 140 | 163 | 88 | 121 | 107 | 191 | 178 | 379 | 131 | 191 | 174 | 182 | 90 | 99 | 183 | 175 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 6 | 1 | 1 | 5 | 5 | 1 | 3 | 0 | 0 | 0 | 8 | 4 | 3 | 0 | 0 | 1 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 0 | 1 | 2 | 13 | 29 | 26 | 37 | 31 | 33 | 19 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 2 | 2 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 4 | 2 | 3 | 11 | 6 | 17 | 8 | 16 | 3 | 7 | 2 | 4 | 2 | 7 | 4 | 4 | 2 | 2 | 0 | 2 | 1 | 3 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 7 | 9 | 1 | 20 | 41 | 25 | 68 | 105 | 55 | 93 | 23 | 69 | 3 | 9 | 4 | 32 | 5 | 20 | 1 | 0 | 1 | 5 | 1 | 2 |
Unique Cell Count | 188 | 197 | 55 | 160 | 273 | 275 | 311 | 330 | 219 | 284 | 137 | 210 | 111 | 197 | 181 | 409 | 139 | 220 | 186 | 199 | 99 | 116 | 210 | 204 |
Labelled Cell Count | 190 | 199 | 56 | 171 | 288 | 297 | 332 | 354 | 252 | 313 | 152 | 232 | 113 | 205 | 184 | 442 | 147 | 236 | 186 | 199 | 99 | 116 | 210 | 204 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 9.2 | 7.9 | 7.8 | 9.5 | 9.3 | 9.6 | 9.8 | 9.0 | 10.2 | 10.5 | 11.4 | 9.4 | 13.2 | 14.9 | 21.8 | 21.2 | 21.2 | 13.0 | 12.2 | 15.9 |
Std Deviation (1e-4) | 1.8 | 1.6 | 1.5 | 2.3 | 2.1 | 2.1 | 2.7 | 2.7 | 2.5 | 3.1 | 3.7 | 4.1 | 1.9 | 6.2 | 4.5 | 5.4 | 4.7 | 5.5 | 11.1 | 8.7 | 11.5 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.26 | 0.22 | 0.28 | 0.3 | 0.18 | 0.36 | 0.41 | 0.52 | 0.25 | 0.74 | 0.91 | 1.46 | 1.42 | 1.42 | 0.71 | 0.62 | 1.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -0.5 | -1.0 | -2.7 | -4.2 | -3.6 | -4.4 | -3.4 | -4.3 | 1.9 | 2.4 | 3.1 | 0.9 | 1.3 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.3 | 2.6 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.3 | 2.7 | 0 | 3.5 | 4.6 | 3.8 | 4.7 | 2.8 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.0283 | 11.5793 | 9.0099 | 8.3341 | 6.7892 | 8.7179 | 8.8666 | 8.926 | 8.9143 | 7.4518 | 6.3968 | 8.1206 | 6.2186 | 8.0968 | 8.0876 | 6.4209 | 5.8615 | 7.7373 |
Actin | 0.0149 | 0.0006 | 0.0039 | 0.0225 | 0.0037 | 0.0008 | 0.0089 | 0.0004 | 0.0002 | 0.0012 | 0.0024 | 0.005 | 0.034 | 0.004 | 0.0235 | 0.0221 | 0.0731 | 0.0048 |
Bud | 0.0004 | 0.0031 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0003 | 0.0018 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0004 |
Bud Neck | 0.0011 | 0.0004 | 0.001 | 0.0011 | 0.0012 | 0.0015 | 0.0005 | 0.0003 | 0.0004 | 0.001 | 0.001 | 0.0014 | 0.0014 | 0.0002 | 0.0004 | 0.0022 | 0.0006 | 0.0017 |
Bud Periphery | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0029 | 0.0001 | 0.0006 | 0.0009 | 0.0003 | 0.0002 |
Bud Site | 0.0023 | 0.001 | 0.0011 | 0.0003 | 0.0008 | 0.0001 | 0.0029 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0031 | 0.0003 | 0.0023 | 0.0018 | 0.0009 | 0.0009 |
Cell Periphery | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.7765 | 0.9374 | 0.8572 | 0.8474 | 0.6697 | 0.8832 | 0.8756 | 0.9573 | 0.9288 | 0.8366 | 0.8033 | 0.8807 | 0.6863 | 0.9434 | 0.8845 | 0.7627 | 0.8067 | 0.8837 |
Cytoplasmic Foci | 0.0232 | 0.0031 | 0.0137 | 0.0077 | 0.0598 | 0.0086 | 0.0037 | 0.002 | 0.0025 | 0.0165 | 0.016 | 0.0069 | 0.0359 | 0.0055 | 0.0105 | 0.0209 | 0.005 | 0.0075 |
Eisosomes | 0.0006 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0069 | 0.0012 | 0.0027 | 0.0024 | 0.0062 | 0.0022 | 0.0053 | 0.0011 | 0.0023 | 0.003 | 0.0381 | 0.0022 | 0.0078 | 0.0013 | 0.0024 | 0.0045 | 0.001 | 0.003 |
Endosome | 0.0261 | 0.0014 | 0.0152 | 0.0033 | 0.0845 | 0.0068 | 0.0045 | 0.0006 | 0.0024 | 0.0026 | 0.0228 | 0.0062 | 0.053 | 0.003 | 0.0093 | 0.0271 | 0.0109 | 0.0182 |
Golgi | 0.0102 | 0.0001 | 0.0081 | 0.0015 | 0.0386 | 0.0011 | 0.0016 | 0 | 0.0001 | 0.0003 | 0.0037 | 0.0015 | 0.0162 | 0.0023 | 0.0069 | 0.0127 | 0.0161 | 0.0047 |
Lipid Particles | 0.0148 | 0 | 0.0019 | 0.0009 | 0.0057 | 0.0031 | 0.002 | 0 | 0 | 0.0017 | 0.0013 | 0.0003 | 0.0248 | 0.0011 | 0.0069 | 0.029 | 0.0357 | 0.0019 |
Mitochondria | 0.0072 | 0.0002 | 0.0045 | 0.0009 | 0.0361 | 0.0041 | 0.0015 | 0.0001 | 0.0002 | 0.0004 | 0.0022 | 0.0019 | 0.0074 | 0.0021 | 0.004 | 0.0211 | 0.0135 | 0.0124 |
None | 0.016 | 0.0081 | 0.0134 | 0.0197 | 0.0065 | 0.0071 | 0.0042 | 0.0019 | 0.0019 | 0.0054 | 0.0094 | 0.0021 | 0.007 | 0.0013 | 0.0026 | 0.0023 | 0.0012 | 0.0016 |
Nuclear Periphery | 0.0077 | 0.0028 | 0.0051 | 0.0051 | 0.0112 | 0.0055 | 0.0073 | 0.0025 | 0.004 | 0.0078 | 0.0118 | 0.0046 | 0.0107 | 0.0029 | 0.0055 | 0.0232 | 0.0022 | 0.0081 |
Nucleolus | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 |
Nucleus | 0.0728 | 0.0374 | 0.0627 | 0.0814 | 0.0418 | 0.0687 | 0.0731 | 0.0321 | 0.0551 | 0.0933 | 0.0738 | 0.0803 | 0.058 | 0.0284 | 0.0281 | 0.0398 | 0.0195 | 0.0443 |
Peroxisomes | 0.011 | 0.0001 | 0.0033 | 0.001 | 0.0174 | 0.0019 | 0.0035 | 0.0001 | 0.0001 | 0.0017 | 0.0004 | 0.0015 | 0.0263 | 0.0023 | 0.0071 | 0.0151 | 0.0102 | 0.0006 |
Punctate Nuclear | 0.0038 | 0.0013 | 0.0029 | 0.0027 | 0.0046 | 0.0029 | 0.002 | 0.0008 | 0.0009 | 0.024 | 0.0098 | 0.0032 | 0.0146 | 0.0007 | 0.0026 | 0.0084 | 0.0008 | 0.0029 |
Vacuole | 0.0022 | 0.0009 | 0.0018 | 0.0011 | 0.0055 | 0.0012 | 0.0021 | 0.0004 | 0.0007 | 0.0015 | 0.0022 | 0.001 | 0.006 | 0.0005 | 0.0009 | 0.0025 | 0.0007 | 0.0017 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0004 | 0.0002 | 0.0032 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0017 | 0.0002 | 0.0004 | 0.0017 | 0.0008 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.5318 | 17.7979 | 23.6332 | 39.9161 | 22.417 | 29.324 | 33.8598 | 36.437 | 47.7164 | 37.1585 |
Translational Efficiency | 1.6113 | 1.6653 | 1.5327 | 1.3873 | 1.8531 | 2.3076 | 1.3059 | 1.4085 | 1.5635 | 1.4377 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
752 | 669 | 427 | 1976 | 1989 | 1734 | 104 | 1386 | 2741 | 2403 | 531 | 3362 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 774.49 | 958.57 | 912.79 | 1605.46 | 857.91 | 921.15 | 2063.32 | 1604.86 | 835.02 | 931.57 | 1138.13 | 1605.21 |
Standard Deviation | 187.36 | 207.99 | 314.10 | 362.68 | 138.95 | 128.82 | 488.02 | 460.81 | 158.20 | 155.88 | 578.33 | 406.02 |
Intensity Change Log 2 | — | 0.307637 | 0.237036 | 1.051668 | — | 0.102610 | 1.266069 | 0.903549 | — | 0.203523 | 0.866433 | 0.975722 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000172 | 0.000328 | 0.000481 | 0.000596 | 0.000114 | 0.000344 | 0.000593 | 0.003576 | 0.000130 | 0.000340 | 0.000503 | 0.001824 |
Bud Neck | 0.005429 | 0.001452 | 0.001754 | 0.003087 | 0.002701 | 0.003239 | 0.039478 | 0.003600 | 0.003450 | 0.002741 | 0.009142 | 0.003299 |
Bud Site | 0.005611 | 0.006695 | 0.005743 | 0.011016 | 0.004092 | 0.007468 | 0.060375 | 0.018745 | 0.004508 | 0.007253 | 0.016443 | 0.014202 |
Cell Periphery | 0.000155 | 0.000111 | 0.000349 | 0.000133 | 0.000087 | 0.000052 | 0.000341 | 0.000130 | 0.000105 | 0.000068 | 0.000348 | 0.000131 |
Cytoplasm | 0.603564* | 0.752298* | 0.625104* | 0.582525* | 0.730984* | 0.754463* | 0.280788* | 0.546363* | 0.696026* | 0.753861* | 0.557667* | 0.567617* |
Cytoplasmic Foci | 0.192444* | 0.052256 | 0.014175 | 0.008659 | 0.068938 | 0.061778 | 0.037618 | 0.026248 | 0.102822 | 0.059127 | 0.018766 | 0.015910 |
Eisosomes | 0.000088 | 0.000019 | 0.000027 | 0.000005 | 0.000022 | 0.000020 | 0.000021 | 0.000012 | 0.000040 | 0.000020 | 0.000025 | 0.000008 |
Endoplasmic Reticulum | 0.000990 | 0.000954 | 0.002556 | 0.000368 | 0.001023 | 0.000556 | 0.001305 | 0.002130 | 0.001014 | 0.000667 | 0.002311 | 0.001094 |
Endosome | 0.008164 | 0.002891 | 0.001765 | 0.005124 | 0.002389 | 0.003663 | 0.021088 | 0.016963 | 0.003973 | 0.003448 | 0.005550 | 0.010005 |
Golgi | 0.001511 | 0.000390 | 0.000368 | 0.007328 | 0.000841 | 0.001242 | 0.003643 | 0.025314 | 0.001025 | 0.001005 | 0.001010 | 0.014743 |
Lipid Particles | 0.004126 | 0.000714 | 0.000936 | 0.000845 | 0.001071 | 0.001074 | 0.003782 | 0.001558 | 0.001909 | 0.000973 | 0.001493 | 0.001139 |
Mitochondria | 0.004401 | 0.000196 | 0.000304 | 0.000859 | 0.000751 | 0.001937 | 0.000452 | 0.005529 | 0.001752 | 0.001452 | 0.000333 | 0.002784 |
Mitotic Spindle | 0.002086 | 0.008605 | 0.007214 | 0.081388 | 0.004447 | 0.007010 | 0.021099 | 0.151894* | 0.003799 | 0.007454 | 0.009933 | 0.110455* |
None | 0.006478 | 0.017659 | 0.012003 | 0.009169 | 0.005078 | 0.003273 | 0.004673 | 0.008952 | 0.005462 | 0.007278 | 0.010568 | 0.009079 |
Nuclear Periphery | 0.000241 | 0.000233 | 0.000715 | 0.000846 | 0.000259 | 0.000178 | 0.001412 | 0.001482 | 0.000254 | 0.000193 | 0.000851 | 0.001108 |
Nuclear Periphery Foci | 0.001458 | 0.003608 | 0.005121 | 0.002150 | 0.000944 | 0.001117 | 0.012679 | 0.006946 | 0.001085 | 0.001811 | 0.006601 | 0.004127 |
Nucleolus | 0.000596 | 0.001303 | 0.000493 | 0.000248 | 0.000300 | 0.000432 | 0.003370 | 0.000414 | 0.000381 | 0.000674 | 0.001056 | 0.000317 |
Nucleus | 0.110770 | 0.105820 | 0.246044* | 0.247293* | 0.146822* | 0.098216 | 0.108364 | 0.104210 | 0.136931* | 0.100333 | 0.219078* | 0.188306* |
Peroxisomes | 0.002177 | 0.000995 | 0.000187 | 0.000272 | 0.000843 | 0.001355 | 0.003524 | 0.001510 | 0.001209 | 0.001255 | 0.000840 | 0.000782 |
Vacuole | 0.048720 | 0.043067 | 0.074454 | 0.036297 | 0.027899 | 0.051859 | 0.386062* | 0.055838 | 0.033612 | 0.049411 | 0.135485 | 0.044353 |
Vacuole Periphery | 0.000819 | 0.000405 | 0.000208 | 0.001793 | 0.000394 | 0.000723 | 0.009334 | 0.018588 | 0.000511 | 0.000635 | 0.001995 | 0.008717 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.89 | -4.06 | -2.02 | -0.90 | 0.60 | -1.57 | -4.50 | -1.46 | -0.86 | 0.80 | -1.94 | -6.06 | -2.32 | -1.09 | 0.51 |
Bud Neck | 6.22 | 2.48 | 2.54 | -7.69 | -0.30 | -1.00 | -7.07 | -2.77 | -1.44 | 6.91 | 1.87 | -5.89 | -1.16 | -3.13 | 5.70 |
Bud Site | -1.09 | -1.85 | -0.66 | 0.50 | 1.51 | -2.76 | -5.94 | -5.49 | -1.96 | 5.39 | -2.97 | -5.85 | -4.75 | -0.68 | 4.65 |
Cell Periphery | 2.92 | -2.25 | 0.98 | -2.16 | 2.44 | 4.37 | -5.05 | -4.65 | -10.78 | 4.50 | 5.17 | -4.53 | -4.27 | -10.26 | 3.84 |
Cytoplasm | -10.72 | 6.18 | 9.44 | 23.18 | 0.11 | -1.68 | 19.76 | 21.51 | 21.99 | -12.02 | -7.24 | 18.48 | 28.47 | 35.24 | -5.17 |
Cytoplasmic Foci | 18.39 | 25.77 | 27.75 | 12.54 | 5.35 | 1.61 | 3.04 | 19.39 | 17.41 | 3.22 | 12.34 | 23.51 | 32.68 | 23.53 | 4.80 |
Eisosomes | 12.25 | 9.41 | 15.41 | 6.33 | 10.62 | 0.82 | -0.69 | 11.18 | 7.57 | 4.96 | 9.54 | 2.89 | 18.22 | 9.99 | 11.48 |
Endoplasmic Reticulum | -0.31 | -6.50 | 5.84 | 4.99 | 8.71 | 3.26 | -1.06 | 2.35 | 0.12 | 2.63 | 2.89 | -6.25 | 4.52 | 2.78 | 8.70 |
Endosome | 6.82 | 8.88 | 9.36 | 4.40 | 1.91 | -2.08 | -5.82 | -1.42 | 0.54 | 5.41 | 1.44 | -0.94 | 5.66 | 3.07 | 4.35 |
Golgi | 4.56 | 4.84 | 0.74 | -1.65 | -1.87 | -0.69 | -3.94 | -2.55 | -2.29 | -0.62 | 0.24 | 0.80 | -1.99 | -2.22 | -2.65 |
Lipid Particles | 6.32 | 3.95 | 3.70 | -7.81 | -1.13 | 0.08 | -5.20 | -6.50 | -3.49 | 3.73 | 2.78 | -2.10 | -1.96 | -4.27 | 1.06 |
Mitochondria | 2.21 | 2.11 | 1.96 | -6.18 | -3.71 | -1.50 | 0.95 | -0.64 | 1.05 | -1.39 | 0.57 | 2.46 | 1.66 | 1.08 | -2.70 |
Mitotic Spindle | -1.96 | -2.16 | -12.14 | -8.55 | -9.09 | -1.25 | -1.91 | -12.48 | -11.84 | -3.93 | -2.11 | -2.51 | -17.07 | -15.21 | -10.99 |
None | -3.17 | -0.65 | -2.33 | 2.16 | -2.43 | 2.63 | 2.18 | -5.02 | -12.46 | -5.92 | -1.29 | -1.11 | -6.14 | -4.37 | -3.34 |
Nuclear Periphery | -0.65 | -12.52 | -27.86 | -27.73 | -3.78 | 3.15 | -6.67 | -9.35 | -10.11 | 1.43 | 2.53 | -13.99 | -22.19 | -23.74 | -1.62 |
Nuclear Periphery Foci | -4.22 | -4.04 | -3.28 | 2.75 | 2.79 | -1.66 | -4.43 | -6.56 | -5.75 | 3.49 | -4.42 | -6.41 | -8.67 | -3.28 | 4.09 |
Nucleolus | -1.58 | 2.11 | 2.01 | 2.10 | -0.54 | -1.07 | -2.77 | -1.38 | 0.53 | 2.73 | -1.92 | -2.53 | -0.04 | 1.95 | 2.54 |
Nucleus | -0.08 | -14.49 | -23.68 | -22.54 | 1.49 | 11.14 | 1.75 | -0.90 | -10.50 | -2.00 | 9.18 | -11.22 | -19.48 | -28.84 | 3.11 |
Peroxisomes | 2.59 | 5.51 | 5.80 | 2.52 | 1.50 | -1.29 | -2.64 | 1.23 | 2.74 | 3.55 | -0.29 | 1.76 | 3.75 | 3.84 | 3.10 |
Vacuole | -0.82 | -10.26 | -17.74 | -15.96 | 0.54 | -11.51 | -12.86 | -24.56 | -17.91 | 7.42 | -10.21 | -15.88 | -32.64 | -23.72 | 3.63 |
Vacuole Periphery | 2.40 | 4.06 | 2.63 | 0.43 | -1.43 | -1.50 | -5.28 | -2.87 | -2.46 | 2.14 | -0.56 | -3.04 | -2.28 | -1.95 | -0.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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