Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.65 0.63 0.7 0.36 0.36 0.26 0.23 0.16 0.19 0.11 0.14 0.53 0.58 0.58 0.32 0.26 0.34 0.31 0.44 0.44 0.5 0.45 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.11 0 0 0.33 0.23 0.24 0.2 0.65 0.49 0.63 0.6 0 0 0 0.06 0 0 0 0.05 0 0 0.05 0
Nucleus 0.41 0.14 0.32 0.26 0.36 0.47 0.55 0.55 0.48 0.55 0.46 0.49 0.34 0.32 0.27 0.49 0.49 0.48 0.46 0.24 0.23 0.19 0.16 0.12
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.2 0.06 0 0.07 0.05 0.07 0.1 0.06 0.06 0.07 0.07 0.19 0.17 0.19 0.22 0.3 0.21 0.14 0.19 0.17 0.13 0.24 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 0 2
Bud 0 0 0 0 1 0 1 1 3 2 6 1 0 0 0 0 0 0 2 3 1 8 3 10
Bud Neck 0 0 1 0 1 0 4 6 1 0 0 0 0 1 1 0 0 1 1 3 0 4 3 3
Bud Site 0 0 0 0 1 0 2 1 1 4 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 57 198 40 16 39 37 40 56 29 34 15 20 159 181 203 150 108 111 53 161 49 156 115 169
Endoplasmic Reticulum 0 1 1 0 1 0 0 2 0 0 0 0 2 2 0 32 23 16 1 0 0 0 1 1
Endosome 1 2 0 0 0 1 0 3 0 1 0 0 2 0 6 8 14 9 1 2 0 2 1 4
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 3 0 1 0 1 1 0
Mitochondria 20 32 1 1 36 24 37 48 122 88 86 88 1 1 4 30 16 11 3 16 3 8 13 11
Nucleus 58 42 20 6 39 49 86 132 89 100 63 72 101 98 96 225 206 157 78 87 25 58 41 37
Nuclear Periphery 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 4 1 0 0 1 1 0 5 2 1 0 2 8 3 14 4 11
Vac/Vac Membrane 28 60 4 0 8 5 11 23 12 11 9 10 56 52 66 101 126 69 24 69 18 41 60 46
Unique Cell Count 141 304 63 23 108 104 156 241 187 181 136 146 301 311 350 463 423 328 171 366 111 314 258 312
Labelled Cell Count 165 336 67 23 127 116 183 277 259 240 184 196 322 335 384 550 496 377 171 366 111 314 258 312


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.9 4.8 5.0 3.8 4.2 4.3 4.7 3.8 4.1 3.9 4.1 5.5 5.6 5.3 5.1 6.7 6.6 5.7 6.1 6.2
Std Deviation (1e-4) 0.6 1.0 0.7 0.8 1.2 1.1 1.1 1.5 1.2 1.2 0.9 1.4 1.8 1.7 1.8 1.1 2.5 1.7 2.0 2.0 2.3
Intensity Change (Log2) – – – 0.05 -0.32 -0.21 -0.17 -0.04 -0.36 -0.22 -0.32 -0.24 0.19 0.21 0.14 0.08 0.47 0.45 0.25 0.35 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 3.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1184 -0.3826 -0.567 -0.5026 0.0243 -0.4123 5.5504 6.1844 5.7667 5.1112 5.757 6.129 3.2046 3.3693 2.8968 2.8526 2.7545 2.8512
Actin 0.0284 0.0006 0.0004 0.0081 0.0651 0.0009 0.0039 0.0007 0.0026 0.0004 0.0086 0.0006 0.0084 0.0019 0.0185 0.0054 0.0287 0.0034
Bud 0.0006 0.0002 0.0001 0.0005 0.0009 0.0001 0.0034 0.0021 0.0001 0.0001 0.0003 0.0018 0.0003 0.0005 0.0001 0.0002 0.0002 0.0002
Bud Neck 0.0023 0.0006 0.0004 0.0004 0.0038 0.0019 0.0021 0.0007 0.001 0.0011 0.001 0.0029 0.0007 0.0005 0.0006 0.0041 0.0019 0.0043
Bud Periphery 0.0008 0.0001 0.0001 0.0005 0.0018 0.0001 0.0013 0.0005 0.0001 0.0001 0.0003 0.0004 0.0003 0.0005 0.0002 0.0003 0.0002 0.0003
Bud Site 0.005 0.0006 0.0004 0.0005 0.0006 0.0001 0.0035 0.0023 0.0025 0.0001 0.0009 0.0002 0.0008 0.0017 0.0006 0.0017 0.0006 0.0003
Cell Periphery 0.0002 0 0 0.0001 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0953 0.1429 0.0814 0.0366 0.1363 0.0979 0.0859 0.1096 0.098 0.1385 0.0928 0.0953 0.1083 0.1431 0.0943 0.1057 0.1066 0.1084
Cytoplasmic Foci 0.0213 0.004 0.0044 0.0025 0.0089 0.0045 0.0032 0.003 0.0033 0.0013 0.0067 0.0027 0.0095 0.0038 0.0052 0.0015 0.0035 0.0042
Eisosomes 0.0002 0 0 0.0001 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0003 0
Endoplasmic Reticulum 0.0043 0.0061 0.0046 0.0115 0.0156 0.0029 0.0034 0.0016 0.002 0.0028 0.0041 0.002 0.0055 0.003 0.0041 0.0028 0.0026 0.0025
Endosome 0.0272 0.0036 0.0079 0.0167 0.0394 0.0048 0.0068 0.0019 0.003 0.0023 0.0102 0.0022 0.0118 0.0033 0.0051 0.0054 0.0036 0.0054
Golgi 0.0108 0.0001 0.0002 0.0063 0.0075 0.001 0.0014 0.0001 0.0005 0 0.0011 0 0.0031 0.0026 0.0017 0.001 0.0039 0.001
Lipid Particles 0.0153 0.0007 0.0007 0.0008 0.0052 0.002 0.0017 0.0003 0.0009 0.0001 0.0029 0.0001 0.0077 0.003 0.0017 0.0018 0.0043 0.0012
Mitochondria 0.0078 0.0006 0.0005 0.0066 0.0048 0.003 0.0013 0.0008 0.0005 0.0008 0.0008 0.0004 0.0027 0.0036 0.0009 0.0015 0.0013 0.0016
None 0.123 0.1332 0.1202 0.07 0.0712 0.1025 0.1527 0.0803 0.1413 0.1226 0.0623 0.1194 0.2646 0.2489 0.2367 0.1884 0.257 0.171
Nuclear Periphery 0.0279 0.0235 0.0327 0.0303 0.0509 0.0142 0.0224 0.0151 0.0105 0.0372 0.0348 0.0097 0.0208 0.0092 0.0161 0.0107 0.0108 0.0123
Nucleolus 0.0055 0.0032 0.0029 0.0038 0.0023 0.0045 0.004 0.0038 0.0037 0.0032 0.0052 0.0059 0.0039 0.0033 0.0038 0.0043 0.0045 0.0063
Nucleus 0.5761 0.6407 0.705 0.7558 0.5522 0.7228 0.6654 0.732 0.6894 0.6492 0.7279 0.7119 0.5142 0.5474 0.5593 0.6511 0.5184 0.6363
Peroxisomes 0.0074 0.0002 0.0001 0.0002 0.0021 0.0037 0.0004 0.0002 0.0003 0.0001 0.0023 0.0001 0.0101 0.0084 0.0029 0.001 0.0027 0.0015
Punctate Nuclear 0.0359 0.0368 0.0352 0.0453 0.0229 0.0317 0.0336 0.0428 0.0385 0.0385 0.0352 0.043 0.0238 0.0127 0.0438 0.0113 0.0468 0.0373
Vacuole 0.0029 0.0018 0.002 0.0018 0.0052 0.001 0.0024 0.0019 0.0013 0.0012 0.0018 0.0013 0.0023 0.0022 0.0033 0.0011 0.0016 0.0019
Vacuole Periphery 0.0016 0.0003 0.0007 0.0016 0.0026 0.0002 0.0012 0.0004 0.0003 0.0005 0.0007 0.0002 0.0008 0.0003 0.0005 0.0004 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3684 4.7824 7.9847 5.0467 6.9363 9.357 4.2311 6.9352 6.3885 6.2573
Translational Efficiency 0.6215 1.4953 0.6372 1.896 0.9439 1.0621 1.3403 1.0607 1.4099 1.0408

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
37 1095 2779 1713 1614 1997 2285 1644 1651 3092 5064 3357

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.05 749.31 831.27 812.36 633.46 803.58 800.51 809.92 636.48 784.36 817.39 811.17
Standard Deviation 104.03 89.83 107.53 126.79 76.43 100.29 107.82 122.10 79.68 100.13 108.74 124.52
Intensity Change Log 2 — -0.035638 0.114117 0.080919 — 0.343188 0.337666 0.354526 — 0.147974 0.219465 0.211041

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000640 0.000378 0.000191 0.001055 0.000031 0.000127 0.000196 0.001515 0.000045 0.000216 0.000193 0.001280
Bud Neck 0.007473 0.014850 0.013040 0.017961 0.003737 0.024283 0.011875 0.009134 0.003821 0.020943 0.012514 0.013638
Bud Site 0.008538 0.005570 0.004131 0.017349 0.000737 0.010174 0.008673 0.017156 0.000912 0.008544 0.006180 0.017254
Cell Periphery 0.000816 0.000193 0.000127 0.000232 0.000121 0.000274 0.000198 0.000226 0.000137 0.000245 0.000159 0.000229
Cytoplasm 0.104013* 0.305442* 0.074036 0.204978* 0.378123* 0.375699* 0.189365* 0.194154* 0.371980* 0.350818* 0.126075* 0.199677*
Cytoplasmic Foci 0.009181 0.106538 0.000431 0.004283 0.057929 0.084490 0.002638 0.005258 0.056836 0.092298 0.001427 0.004760
Eisosomes 0.000086 0.000075 0.000013 0.000034 0.000036 0.000074 0.000020 0.000029 0.000037 0.000074 0.000016 0.000032
Endoplasmic Reticulum 0.007621 0.002955 0.002735 0.005426 0.000538 0.001194 0.002532 0.003656 0.000697 0.001818 0.002643 0.004559
Endosome 0.002590 0.006170 0.000535 0.005098 0.001043 0.006912 0.001087 0.002384 0.001078 0.006649 0.000784 0.003769
Golgi 0.000587 0.001438 0.000042 0.002557 0.000135 0.001131 0.000148 0.002737 0.000145 0.001240 0.000090 0.002645
Lipid Particles 0.024830 0.005540 0.000186 0.001433 0.002088 0.005860 0.000463 0.000718 0.002597 0.005747 0.000311 0.001083
Mitochondria 0.005164 0.006116 0.001678 0.007700 0.000670 0.010034 0.001537 0.002193 0.000770 0.008647 0.001614 0.005003
Mitotic Spindle 0.001495 0.009062 0.006767 0.050345 0.000071 0.007771 0.014249 0.016137 0.000103 0.008228 0.010143 0.033593
None 0.011231 0.007337 0.005005 0.013412 0.008383 0.009074 0.006818 0.014521 0.008447 0.008459 0.005824 0.013955
Nuclear Periphery 0.003258 0.001621 0.001961 0.003570 0.000634 0.001603 0.002009 0.001914 0.000692 0.001609 0.001983 0.002759
Nuclear Periphery Foci 0.022998 0.000783 0.000182 0.001404 0.000229 0.000279 0.000194 0.000631 0.000739 0.000458 0.000187 0.001025
Nucleolus 0.013080 0.003012 0.000626 0.001271 0.001248 0.003542 0.000635 0.001515 0.001513 0.003354 0.000630 0.001390
Nucleus 0.749625* 0.490447* 0.882716* 0.611513* 0.536203* 0.419357* 0.740137* 0.700546* 0.540986* 0.444533* 0.818381* 0.655114*
Peroxisomes 0.002454 0.009196 0.000052 0.000473 0.000806 0.005627 0.000171 0.000421 0.000843 0.006891 0.000106 0.000447
Vacuole 0.022864 0.022449 0.005366 0.045114 0.007110 0.030403 0.016342 0.023066 0.007463 0.027586 0.010319 0.034317
Vacuole Periphery 0.001458 0.000828 0.000180 0.004796 0.000130 0.002092 0.000713 0.002089 0.000160 0.001644 0.000420 0.003470

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.94 1.50 0.06 -1.44 -2.53 -6.10 -11.03 -2.37 -2.15 -1.80 -5.42 -14.98 -3.59 -2.77 -2.63
Bud Neck -1.53 -1.33 -1.49 0.15 -0.58 -12.57 -10.30 -7.93 7.98 2.91 -14.05 -12.68 -10.62 6.20 1.20
Bud Site 0.56 0.83 -0.42 -3.90 -5.18 -8.77 -6.32 -7.14 -1.24 -2.71 -10.09 -7.89 -10.52 -2.70 -5.43
Cell Periphery 1.53 1.71 1.62 2.46 -2.56 -6.96 -2.87 -3.71 4.07 -0.94 -5.75 -0.17 -1.88 5.01 -2.56
Cytoplasm -5.72 1.00 -1.56 15.51 -16.11 0.23 26.24 23.83 25.20 -0.98 2.51 35.47 26.71 30.42 -11.79
Cytoplasmic Foci -17.04 3.52 2.52 20.37 -9.54 -6.30 20.37 19.43 26.55 -3.53 -9.16 20.86 19.90 33.40 -7.65
Eisosomes 0.46 3.00 1.79 7.41 -16.54 -10.31 6.42 0.52 10.77 -6.22 -12.40 10.37 -1.38 12.51 -16.65
Endoplasmic Reticulum 2.40 2.03 0.79 -7.24 -6.37 -5.50 -19.94 -16.37 -12.94 -3.07 -8.40 -24.22 -21.11 -14.79 -6.93
Endosome -3.79 2.76 0.23 5.82 -6.76 -11.35 -0.02 -2.66 9.23 -2.14 -13.91 2.05 -4.82 10.66 -6.30
Golgi -2.19 2.39 -0.51 1.10 -1.85 -5.20 0.42 -1.84 -0.44 -1.86 -6.64 2.28 -2.40 0.19 -2.57
Lipid Particles 1.26 1.62 1.56 8.71 -3.93 -7.50 9.43 8.11 10.80 -2.76 -6.14 6.05 4.32 13.26 -4.74
Mitochondria -0.46 1.95 0.67 2.00 -3.29 -8.68 -7.83 -6.41 7.43 -0.66 -10.29 -7.72 -6.15 6.83 -3.33
Mitotic Spindle -3.70 -5.01 -9.36 -5.99 -6.86 -5.64 -8.44 -6.95 -2.68 0.47 -7.34 -11.18 -12.28 -6.72 -5.18
None 0.86 1.45 0.61 -0.74 -3.78 -0.53 2.07 -2.09 -1.50 -4.03 0.01 3.25 -1.28 -1.33 -5.58
Nuclear Periphery 1.41 0.58 -1.34 -11.87 -9.42 -2.94 -21.28 -15.36 -3.45 1.37 -4.19 -26.81 -22.56 -8.85 -7.00
Nuclear Periphery Foci 1.57 1.61 1.55 -1.64 -6.41 -0.58 -0.70 -4.24 -3.84 -4.03 0.85 1.49 -0.42 -4.51 -7.57
Nucleolus 1.98 2.47 2.38 5.74 -3.26 -5.79 3.32 -0.83 4.33 -2.45 -5.95 4.73 0.91 6.66 -3.65
Nucleus 4.68 -2.46 1.51 -14.21 26.76 11.50 -23.33 -17.02 -29.51 4.42 10.40 -34.47 -16.09 -31.77 22.10
Peroxisomes -4.86 2.69 2.40 7.91 -2.06 -7.31 4.83 3.73 8.24 -1.20 -10.24 6.13 4.15 11.40 -2.45
Vacuole 0.12 1.23 -4.12 -14.77 -20.12 -13.16 -12.07 -13.81 -4.59 -6.37 -15.05 -9.52 -24.06 -14.00 -19.94
Vacuole Periphery 1.17 2.45 -0.22 -1.77 -3.35 -5.83 -1.66 -2.45 1.63 -1.51 -6.76 -1.48 -4.06 0.64 -3.45
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages nucleus (26%), cytoplasm (18%), mixed (50%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.0004)
Subcompartmental Group N/A

Cmg1

Cmg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cmg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available