Standard name
Human Ortholog
Description Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.65 0.77 0.74 0.71 0.6 0.65 0.73 0.81 0.84 0.78 0.87 0.93 0.98 0.99 0.61 0.56 0.58 0.1 0.15 0.13 0.19 0.25 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.07 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.05 0 0 0 0 0.22 0.09 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0
Nucleus 0 0 0.06 0.16 0.08 0.19 0.14 0.12 0.09 0.08 0 0 0 0 0 0.12 0.12 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Vac/Vac Membrane 0.22 0.27 0.17 0.18 0.29 0.23 0.29 0.19 0.19 0.13 0.06 0.08 0.08 0 0 0.37 0.4 0.4 0.63 0.55 0.68 0.63 0.55 0.39
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 1 1 1 0 0 0 0 0 0 1 0 0 2 0 1 4 1 2 0 1 1
Bud 0 4 1 1 0 4 3 2 0 1 0 2 0 0 1 1 0 0 6 7 1 0 3 1
Bud Neck 1 0 1 0 1 1 1 2 1 4 1 5 0 0 1 0 1 0 1 4 2 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 1 0 1 1 1 1 0 0 1 0 0 0 2 0 0 0 0 1 0 0 0
Cytoplasm 118 88 97 108 112 100 137 166 175 183 91 181 166 172 221 140 116 102 19 25 18 6 17 27
Endoplasmic Reticulum 3 5 3 0 2 0 1 1 3 5 0 0 0 3 0 8 14 12 2 1 0 0 1 0
Endosome 10 4 1 0 2 2 2 1 0 1 0 2 2 0 2 5 9 13 13 11 6 1 1 1
Golgi 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Mitochondria 12 2 0 1 0 8 1 4 2 3 26 18 0 0 0 2 8 2 9 7 4 1 1 2
Nucleus 5 4 8 23 13 31 30 27 20 18 4 3 5 1 0 27 26 22 0 1 2 0 0 1
Nuclear Periphery 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 0 0 0 1 2 0 1 1 1 7 1 0 0 0 3 0 6 8 3 0 0 4
Vac/Vac Membrane 38 37 21 27 46 38 60 44 41 28 7 16 14 5 3 86 83 70 121 96 99 21 36 28
Unique Cell Count 172 135 126 146 157 166 210 226 216 219 117 208 178 176 223 231 208 176 191 175 146 34 67 74
Labelled Cell Count 191 149 137 162 178 188 239 250 245 244 130 236 189 181 228 274 263 223 191 175 146 34 67 74


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.8 5.4 5.8 6.5 5.2 5.3 5.5 4.4 4.4 4.1 4.0 5.5 5.8 5.3 6.5 6.5 6.4 5.5 5.4 6.3
Std Deviation (1e-4) 1.0 1.0 0.8 2.1 2.2 1.6 2.2 1.8 1.4 1.1 1.9 1.0 1.3 2.1 1.6 1.4 1.4 1.6 0.7 1.5 2.3
Intensity Change (Log2) – – – 0.1 0.26 -0.07 -0.03 0.03 -0.28 -0.31 -0.41 -0.44 0.02 0.11 -0.03 0.28 0.26 0.24 0.03 -0.0 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.1 4.4 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1906 -0.3677 0.5391 1.4656 0.3307 -0.0438 1.2434 -0.3992 0.2901 1.7303 -0.0817 -0.0603 0.4793 -0.9968 -1.3183 -0.3172 -0.3264 -1.058
Actin 0.0602 0.0012 0.0665 0.1298 0.0313 0.0029 0.0579 0.0019 0.04 0.0499 0.0227 0.035 0.0752 0.0001 0.0564 0.1055 0.1398 0.0008
Bud 0.002 0.0084 0.0053 0.0147 0.0034 0.0038 0.0016 0.0064 0.0038 0.0025 0.001 0.0042 0.0013 0.0138 0.0063 0.0027 0.0028 0.0005
Bud Neck 0.0137 0.0013 0.0038 0.0023 0.0011 0.009 0.0139 0.001 0.0018 0.0018 0.0006 0.0059 0.0213 0.0004 0.0012 0.003 0.0016 0.0045
Bud Periphery 0.0048 0.01 0.0111 0.0175 0.0118 0.0052 0.0036 0.0094 0.0081 0.0046 0.0027 0.0068 0.0021 0.0059 0.0102 0.0045 0.0081 0.0016
Bud Site 0.0179 0.0177 0.0347 0.046 0.0009 0.0013 0.0431 0.0181 0.0182 0.003 0.0017 0.0017 0.0155 0.0048 0.0066 0.0115 0.0022 0.0015
Cell Periphery 0.0018 0.001 0.0012 0.0011 0.0003 0.0007 0.0029 0.0017 0.0016 0.0004 0.0006 0.0009 0.0009 0.0018 0.0008 0.0004 0.0004 0.0004
Cytoplasm 0.0744 0.1321 0.0929 0.1646 0.0393 0.1358 0.052 0.1341 0.0568 0.0093 0.0409 0.1057 0.0383 0.134 0.1384 0.0316 0.1009 0.2131
Cytoplasmic Foci 0.1308 0.0666 0.074 0.059 0.1114 0.1088 0.1501 0.0514 0.0477 0.1048 0.0693 0.0749 0.0967 0.0462 0.0583 0.0296 0.0767 0.0614
Eisosomes 0.0011 0.0003 0.0008 0.0004 0.0004 0.0001 0.0012 0.0002 0.0012 0.0003 0.0002 0.0003 0.0013 0.0001 0.0002 0.0006 0.0004 0.0001
Endoplasmic Reticulum 0.0047 0.0046 0.0041 0.008 0.0052 0.0039 0.0062 0.0069 0.0038 0.0085 0.002 0.0032 0.0127 0.0041 0.0038 0.0003 0.0029 0.0049
Endosome 0.2463 0.1041 0.1809 0.1508 0.2469 0.1916 0.1769 0.0458 0.078 0.3154 0.0983 0.1417 0.1561 0.0735 0.1121 0.158 0.2216 0.1572
Golgi 0.0323 0.0046 0.0209 0.0462 0.0386 0.0133 0.0442 0.0056 0.0293 0.1635 0.0414 0.0114 0.0368 0.0014 0.0096 0.0151 0.0864 0.0044
Lipid Particles 0.0382 0.0203 0.0256 0.053 0.1106 0.0291 0.0997 0.0147 0.0474 0.1046 0.054 0.0355 0.1034 0.0153 0.0314 0.0081 0.0228 0.0159
Mitochondria 0.0226 0.0056 0.0262 0.0182 0.0686 0.0142 0.0415 0.0188 0.069 0.0534 0.0308 0.0407 0.0598 0.0055 0.0479 0.2169 0.0374 0.0295
None 0.2034 0.4465 0.2754 0.1715 0.1422 0.3304 0.1112 0.5861 0.47 0.0034 0.427 0.3744 0.0904 0.5098 0.3119 0.3678 0.1725 0.3289
Nuclear Periphery 0.0108 0.0059 0.0104 0.0144 0.0321 0.0052 0.0174 0.0027 0.0069 0.0168 0.0016 0.0071 0.0255 0.0029 0.0021 0.0003 0.0027 0.004
Nucleolus 0.0062 0.0059 0.0064 0.0026 0.0021 0.0058 0.0048 0.005 0.0025 0.0017 0.0027 0.0055 0.003 0.0024 0.0013 0.0004 0.0007 0.0008
Nucleus 0.0068 0.0106 0.0397 0.0059 0.0042 0.0092 0.0043 0.0043 0.0047 0.004 0.002 0.0091 0.0124 0.0049 0.0039 0.0004 0.0012 0.0045
Peroxisomes 0.0196 0.0084 0.0242 0.0147 0.0705 0.0309 0.062 0.006 0.0203 0.037 0.0435 0.0241 0.0904 0.0007 0.0199 0.0184 0.0167 0.0041
Punctate Nuclear 0.0091 0.0071 0.0093 0.0093 0.0102 0.0054 0.0044 0.003 0.0076 0.0038 0.0027 0.0111 0.0104 0.0048 0.001 0.0003 0.0023 0.0011
Vacuole 0.0839 0.1246 0.075 0.0553 0.0557 0.0818 0.0855 0.0695 0.0692 0.0937 0.1423 0.083 0.1365 0.1528 0.1504 0.0219 0.0786 0.1365
Vacuole Periphery 0.0096 0.013 0.0115 0.0146 0.0133 0.0116 0.0154 0.0072 0.012 0.0179 0.0118 0.0177 0.0099 0.0146 0.0264 0.0027 0.0214 0.0242

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 88.5206 23.36 26.0012 95.1401 297.7266 94.0215 24.6526 24.5099 53.4643 444.0149
Translational Efficiency 0.5691 0.5668 0.4371 0.2991 0.9456 0.7797 0.6357 0.5197 0.3878 0.8136

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1399 1633 1395 704 2052 1022 283 767 3451 2655 1678 1471

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.32 720.80 843.33 806.80 704.85 693.57 871.49 806.50 668.56 710.32 848.08 806.64
Standard Deviation 80.27 103.75 108.67 113.93 83.46 107.50 110.00 123.58 93.20 106.04 109.41 119.06
Intensity Change Log 2 — 0.228262 0.454760 0.390874 — -0.023275 0.306168 0.194358 — 0.099436 0.377333 0.289291

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000295 0.000202 0.000514 0.001150 0.000193 0.000649 0.000157 0.000792 0.000234 0.000374 0.000454 0.000963
Bud Neck 0.120617 0.030956 0.019050 0.016206 0.046204 0.055315 0.003973 0.025579 0.076370 0.040333 0.016508 0.021093
Bud Site 0.055427 0.013722 0.023863 0.076436 0.012627 0.035274 0.006313 0.064567 0.029978 0.022018 0.020903 0.070247
Cell Periphery 0.003281 0.000083 0.000099 0.000270 0.000165 0.000365 0.000545 0.000317 0.001428 0.000192 0.000175 0.000295
Cytoplasm 0.300144* 0.488560* 0.371896* 0.536501* 0.442313* 0.216870* 0.374178* 0.415111* 0.384680* 0.383977* 0.372281* 0.473207*
Cytoplasmic Foci 0.126316 0.130702 0.007924 0.016250 0.144820 0.140620 0.035949 0.036813 0.137319 0.134520 0.012651 0.026972
Eisosomes 0.000305 0.000064 0.000024 0.000048 0.000141 0.000116 0.000499 0.000063 0.000208 0.000084 0.000104 0.000056
Endoplasmic Reticulum 0.001955 0.000597 0.003940 0.003794 0.001020 0.001642 0.002024 0.002909 0.001399 0.000999 0.003617 0.003332
Endosome 0.038223 0.032137 0.004464 0.004581 0.034406 0.108699 0.013075 0.027195 0.035953 0.061608 0.005916 0.016372
Golgi 0.002201 0.001451 0.000208 0.000943 0.003165 0.013258 0.000078 0.004445 0.002774 0.005996 0.000186 0.002769
Lipid Particles 0.005959 0.003051 0.000967 0.001417 0.003849 0.005834 0.002691 0.004964 0.004705 0.004122 0.001258 0.003266
Mitochondria 0.007802 0.002290 0.001476 0.000979 0.003921 0.018279 0.000181 0.005714 0.005494 0.008445 0.001257 0.003448
Mitotic Spindle 0.000656 0.007634 0.009950 0.027231 0.001504 0.009652 0.000820 0.025066 0.001160 0.008410 0.008410 0.026102
None 0.005361 0.002443 0.004544 0.006439 0.002441 0.003606 0.006247 0.002965 0.003625 0.002891 0.004831 0.004628
Nuclear Periphery 0.000240 0.000336 0.002749 0.000995 0.000269 0.000442 0.001068 0.003127 0.000257 0.000376 0.002465 0.002107
Nuclear Periphery Foci 0.000274 0.000376 0.001337 0.001259 0.000272 0.000591 0.001852 0.001280 0.000273 0.000459 0.001424 0.001270
Nucleolus 0.001944 0.001625 0.001136 0.001302 0.001067 0.002445 0.000640 0.001034 0.001422 0.001941 0.001052 0.001162
Nucleus 0.060006 0.083745 0.466708* 0.157938* 0.079148 0.036426 0.425435* 0.160806* 0.071388 0.065530 0.459747* 0.159434*
Peroxisomes 0.008874 0.005953 0.001097 0.001351 0.008749 0.016327 0.000766 0.006796 0.008800 0.009947 0.001041 0.004191
Vacuole 0.255565* 0.192010 0.077356 0.142888 0.212022* 0.320055* 0.123299 0.202093* 0.229674* 0.241299* 0.085104 0.173758
Vacuole Periphery 0.004557 0.002063 0.000696 0.002023 0.001703 0.013533 0.000211 0.008364 0.002860 0.006478 0.000614 0.005329

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.39 -1.45 -2.30 -2.68 -1.41 -2.05 0.22 -3.44 0.15 -3.24 -1.49 -1.92 -3.72 -2.24 -1.81
Bud Neck 15.68 17.36 19.41 7.31 3.74 -2.06 16.02 10.83 10.23 -4.40 11.14 18.41 21.65 12.15 2.71
Bud Site 11.79 8.71 3.80 -4.32 -2.74 -7.79 2.50 -4.30 1.08 -5.12 4.24 5.15 -0.50 -3.09 -4.05
Cell Periphery 7.32 7.27 7.12 -3.40 -2.48 -3.12 -3.32 -0.17 2.87 3.10 6.85 7.10 7.00 0.74 -0.63
Cytoplasm -17.84 -2.32 -9.45 4.69 -7.74 21.89 5.98 6.27 -11.50 -1.38 0.09 6.97 -1.17 -1.17 -6.82
Cytoplasmic Foci -0.53 24.84 22.82 27.90 -4.45 0.64 21.48 27.60 19.20 2.15 0.79 40.67 36.40 32.71 -5.43
Eisosomes 10.85 12.39 10.82 -0.00 -8.49 1.40 -1.93 6.54 3.12 2.59 9.98 5.55 12.57 2.67 1.05
Endoplasmic Reticulum 4.62 -7.73 -1.97 -4.91 3.16 -2.42 -4.38 -4.28 -2.60 0.42 2.28 -11.80 -4.35 -6.00 4.53
Endosome 2.68 16.73 17.42 14.63 1.91 -14.66 7.08 8.76 18.79 0.04 -9.48 21.44 17.17 21.22 -1.72
Golgi 2.73 8.57 5.95 3.34 -0.88 -5.47 6.10 1.88 6.18 -2.58 -4.10 8.23 3.79 6.29 -2.60
Lipid Particles 5.84 10.62 9.56 5.00 -1.72 -2.31 2.66 0.90 2.14 -0.04 1.44 12.49 3.81 2.71 -1.07
Mitochondria 4.87 5.50 6.28 5.87 2.28 -6.09 5.56 1.55 6.68 -2.88 -2.65 6.56 5.45 6.98 -0.77
Mitotic Spindle -4.31 -5.95 -4.81 -2.26 -0.99 -4.34 0.36 -5.02 -1.96 -5.07 -5.85 -5.58 -6.91 -3.13 -2.39
None 3.47 1.62 -0.08 -4.32 -2.18 -0.82 -1.25 -1.51 0.01 0.62 1.10 -0.89 -1.41 -2.59 -0.62
Nuclear Periphery -3.21 -12.56 -15.00 -14.11 5.23 -2.69 -10.07 -3.18 -2.94 -1.46 -3.87 -13.46 -5.17 -4.83 1.58
Nuclear Periphery Foci -1.87 -3.59 -5.40 -4.66 0.57 -1.82 -4.69 -5.48 -2.86 2.03 -2.50 -4.46 -7.67 -5.63 1.15
Nucleolus 0.60 0.68 3.41 2.95 2.41 -3.88 1.60 2.35 5.23 0.28 -2.61 -0.34 3.89 5.73 2.90
Nucleus -6.44 -50.49 -19.61 -16.47 22.74 11.51 -17.91 -16.26 -20.92 9.67 1.61 -52.93 -25.05 -25.25 27.26
Peroxisomes 2.70 7.97 8.51 9.35 0.68 -3.99 8.71 3.89 6.66 -2.84 -1.10 11.11 7.85 8.72 -2.19
Vacuole 6.18 17.04 0.27 -4.89 -13.74 -9.84 2.19 -7.38 1.69 -6.75 -1.97 18.56 -5.32 -3.60 -19.25
Vacuole Periphery 5.88 9.80 8.17 3.26 -0.54 -10.55 7.55 -1.07 6.20 -2.25 -7.02 10.12 1.30 5.41 -2.11
Standard name
Human Ortholog
Description Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
Localization
Cell Percentages vacuole (24%), cytoplasm (11%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cts1

Cts1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cts1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available