ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.97 0.94 0.91 0.84 0.86 0.79 0.79 0.73 0.68 0.95 0.99 0.98 0.9 0.94 0.97 0.94 0.97 0.94 0.92 0.92 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.18 0.11 0.25 0.18 0.34 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0.06 0 0.05 0.05 0 0.13 0 0 0 0.11 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 0 1 1 0 0 1 0 0 0 1 0 0 0 0 0
Bud 1 1 0 0 1 1 1 3 4 2 5 11 0 1 0 0 0 1 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 5 1 7 5 5 6 7 11 6 9 1 2 3 12 11 7 0 0 0 0 0 0
Cytoplasm 264 396 226 179 262 218 280 276 225 256 225 231 77 147 150 221 297 310 252 385 224 109 210 246
Endoplasmic Reticulum 1 5 1 0 1 3 2 7 1 6 2 3 0 1 1 8 7 9 3 1 2 2 1 0
Endosome 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 1 0 1 1
Golgi 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 1
Mitochondria 1 0 0 1 10 15 59 35 70 58 104 80 1 0 0 0 1 2 0 0 2 0 3 1
Nucleus 4 1 1 1 2 1 1 10 4 9 3 3 3 0 0 0 1 1 1 2 1 2 1 0
Nuclear Periphery 0 0 0 0 0 2 1 0 1 1 0 2 0 0 0 0 0 0 1 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 1 1 4 13 8 21 10 13 15 9 44 0 0 0 27 23 7 2 4 3 0 4 2
Unique Cell Count 268 401 227 184 278 239 335 322 284 323 307 338 81 148 153 245 317 318 270 399 239 119 229 262
Labelled Cell Count 274 410 234 186 297 254 372 348 325 358 355 385 82 151 155 270 342 337 270 399 239 119 229 262


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 13.9 10.6 9.1 8.7 7.0 6.4 7.1 5.9 6.2 5.6 6.1 9.7 10.8 10.8 18.2 18.4 18.0 11.4 11.3 13.0
Std Deviation (1e-4) – 2.2 1.3 1.1 1.3 1.1 1.1 1.2 1.1 1.0 0.8 1.0 1.7 1.5 1.7 3.9 4.4 3.6 2.3 2.5 2.4
Intensity Change (Log2) – – – -0.21 -0.27 -0.59 -0.73 -0.58 -0.84 -0.76 -0.91 -0.78 -0.12 0.04 0.03 0.79 0.8 0.77 0.11 0.1 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 0 0 0 0.2 0.8 0 0.3 0 0 0 1.6 0.9 0
Cytoplasm -1.9 -3.3 -4.2 -6.2 -5.7 -7.1 -7.1 -8.3 -9.3 -2.7 -0.3 -1.4 -4.5 -3.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 6.7 5.1 8.1 6.8 9.8 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.9 0 3.5 0 2.8 2.9 0 5.4 0 0 0 4.9 3.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6817 9.636 9.4013 8.5491 7.4246 9.1375 6.8162 8.7684 7.8728 7.2084 6.9206 8.0738 5.9135 9.0069 7.4791 8.0286 8.9787 8.3148
Actin 0.0324 0.0019 0.0103 0.0001 0.0155 0.0024 0.0079 0.0006 0.0052 0.026 0.0437 0.0049 0.0898 0.0005 0.0045 0.0001 0.0003 0.0031
Bud 0.0006 0.0021 0.0004 0.0001 0.0014 0.0005 0.0006 0.0001 0.0005 0.0003 0.0007 0.0002 0.0013 0.0073 0.0012 0.0001 0 0.0041
Bud Neck 0.0055 0.0003 0.0005 0.0004 0.0009 0.0008 0.0013 0.0002 0.0027 0.0005 0.0014 0.0027 0.0015 0.0001 0.0019 0.0003 0.0003 0.0033
Bud Periphery 0.0004 0.0017 0.0003 0 0.0019 0.0005 0.0006 0 0.0006 0.0002 0.001 0.0001 0.0014 0.0046 0.0011 0 0 0.009
Bud Site 0.0061 0.0016 0.002 0 0.0022 0.0006 0.0008 0.0005 0.0063 0.0005 0.0021 0.0001 0.0041 0.0016 0.0162 0 0 0.0117
Cell Periphery 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0009 0.0001 0.0001 0 0.0003 0.0001 0.0002 0 0 0.0005
Cytoplasm 0.8604 0.9734 0.9354 0.9913 0.7774 0.9296 0.8642 0.9831 0.9394 0.8698 0.8096 0.9447 0.6203 0.9464 0.8704 0.9162 0.9858 0.8919
Cytoplasmic Foci 0.0207 0.0043 0.0117 0.0022 0.0148 0.008 0.0228 0.0036 0.01 0.0158 0.0106 0.0045 0.0435 0.0027 0.0201 0.0015 0.0019 0.0041
Eisosomes 0.0002 0 0 0 0.0002 0 0 0 0.0002 0.0006 0.0003 0.0001 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0054 0.001 0.0017 0.0007 0.0026 0.0029 0.0067 0.001 0.0018 0.0046 0.0091 0.0057 0.0178 0.0013 0.0029 0.001 0.0042 0.004
Endosome 0.024 0.0027 0.0126 0.0007 0.05 0.0116 0.0274 0.0021 0.0106 0.018 0.056 0.0119 0.0708 0.0005 0.0349 0.0008 0.0015 0.0112
Golgi 0.0081 0.0006 0.0071 0 0.0394 0.0075 0.0063 0.0016 0.0043 0.0063 0.0125 0.0049 0.0349 0.0001 0.0052 0 0.0001 0.0035
Lipid Particles 0.0065 0 0.0038 0 0.0051 0.0058 0.0023 0 0.0011 0.0198 0.0068 0.0018 0.0134 0.0001 0.0065 0.0002 0 0.0002
Mitochondria 0.0025 0.0003 0.002 0.0001 0.0505 0.0144 0.009 0.001 0.005 0.0017 0.0085 0.0022 0.0192 0.0001 0.0016 0.0001 0 0.0077
None 0.0077 0.002 0.0045 0.0014 0.0009 0.0021 0.0136 0.0017 0.0024 0.0109 0.0065 0.0022 0.0071 0.0113 0.0031 0.0761 0.001 0.0012
Nuclear Periphery 0.0053 0.001 0.0008 0.0003 0.001 0.0014 0.0092 0.0005 0.0008 0.0033 0.0083 0.0049 0.0083 0.0014 0.0028 0.0008 0.0012 0.013
Nucleolus 0.0002 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0003 0.0006 0.0002 0.0001 0.0004 0.0001 0.0002 0 0 0.0012
Nucleus 0.0051 0.0058 0.0022 0.0021 0.0026 0.0056 0.0115 0.003 0.0038 0.004 0.0073 0.006 0.0474 0.011 0.0069 0.0019 0.003 0.0181
Peroxisomes 0.0039 0.0001 0.0026 0 0.0047 0.0038 0.0082 0.0002 0.0012 0.005 0.0057 0.0006 0.0074 0 0.0157 0 0 0.0002
Punctate Nuclear 0.0016 0.0002 0.0003 0.0001 0.0003 0.0007 0.0049 0.0003 0.0003 0.006 0.0013 0.0006 0.0035 0.0103 0.0028 0.0001 0.0002 0.001
Vacuole 0.0025 0.0008 0.001 0.0002 0.0201 0.0012 0.0017 0.0003 0.002 0.0045 0.0061 0.0012 0.0046 0.0004 0.0013 0.0005 0.0005 0.0063
Vacuole Periphery 0.0008 0.0002 0.0004 0.0001 0.0082 0.0004 0.0007 0.0001 0.0004 0.0015 0.0021 0.0006 0.0024 0.0001 0.0005 0.0001 0.0001 0.0047

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.8094 27.7072 33.5812 27.9386 25.451 41.3031 29.6489 26.1561 21.7137 28.0679
Translational Efficiency 1.3048 1.9797 1.4796 1.6911 1.7291 0.9916 1.3526 1.5323 1.6184 1.4809

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2284 152 3282 1765 807 1817 425 1948 3091 1969 3707 3713

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1132.53 1199.28 1385.97 1407.66 1011.85 1196.06 1395.13 1491.26 1101.02 1196.31 1387.02 1451.52
Standard Deviation 153.70 191.90 192.81 226.43 200.88 172.66 199.24 221.48 175.50 174.22 193.58 227.70
Intensity Change Log 2 — 0.082619 0.291347 0.313750 — 0.241294 0.463404 0.559536 — 0.159670 0.375095 0.434955

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000068 0.000221 0.000099 0.000638 0.000255 0.000239 0.000415 0.000241 0.000117 0.000238 0.000135 0.000430
Bud Neck 0.000511 0.001135 0.001526 0.002222 0.000498 0.001289 0.002098 0.002672 0.000507 0.001277 0.001592 0.002458
Bud Site 0.001989 0.007314 0.003793 0.015422 0.002959 0.006190 0.012370 0.016301 0.002242 0.006277 0.004776 0.015883
Cell Periphery 0.000029 0.000083 0.000022 0.000115 0.000553 0.000038 0.000133 0.000057 0.000166 0.000041 0.000035 0.000085
Cytoplasm 0.863809* 0.836430* 0.777388* 0.782838* 0.817433* 0.836320* 0.667302* 0.837735* 0.851701* 0.836329* 0.764767* 0.811639*
Cytoplasmic Foci 0.028723 0.058165 0.002736 0.017162 0.040041 0.033293 0.019987 0.014981 0.031678 0.035213 0.004713 0.016018
Eisosomes 0.000003 0.000008 0.000002 0.000006 0.000516 0.000005 0.000018 0.000003 0.000137 0.000005 0.000004 0.000004
Endoplasmic Reticulum 0.000544 0.000553 0.000123 0.000264 0.001831 0.000324 0.001273 0.000106 0.000880 0.000342 0.000255 0.000181
Endosome 0.000836 0.003184 0.000249 0.006852 0.002770 0.003520 0.003195 0.002338 0.001341 0.003494 0.000587 0.004484
Golgi 0.000779 0.000395 0.000194 0.008833 0.000581 0.003727 0.000237 0.003347 0.000727 0.003469 0.000199 0.005955
Lipid Particles 0.000270 0.000529 0.000149 0.001593 0.002491 0.000514 0.001345 0.000600 0.000850 0.000515 0.000286 0.001072
Mitochondria 0.000435 0.000030 0.000327 0.003389 0.000120 0.001340 0.000110 0.000377 0.000353 0.001239 0.000302 0.001809
Mitotic Spindle 0.001350 0.000875 0.003295 0.022311 0.002073 0.016887 0.002629 0.026889 0.001539 0.015651 0.003219 0.024713
None 0.005672 0.002637 0.007863 0.003713 0.008946 0.003632 0.003958 0.002567 0.006526 0.003556 0.007415 0.003112
Nuclear Periphery 0.000081 0.000118 0.000232 0.000302 0.000165 0.000129 0.000599 0.000153 0.000103 0.000128 0.000274 0.000224
Nuclear Periphery Foci 0.001216 0.005174 0.000340 0.002148 0.007432 0.001535 0.008078 0.001071 0.002839 0.001816 0.001227 0.001583
Nucleolus 0.000188 0.000082 0.000131 0.000331 0.000243 0.000543 0.000185 0.000339 0.000203 0.000507 0.000137 0.000335
Nucleus 0.074868 0.042973 0.187984* 0.072657 0.073119 0.056786 0.113913 0.044550 0.074411 0.055719 0.179492* 0.057911
Peroxisomes 0.000117 0.000393 0.000057 0.001980 0.001449 0.000611 0.000172 0.000620 0.000465 0.000595 0.000070 0.001266
Vacuole 0.018116 0.039557 0.013423 0.052437 0.035477 0.031358 0.161794 0.042491 0.022648 0.031991 0.030434 0.047219
Vacuole Periphery 0.000395 0.000144 0.000066 0.004789 0.001050 0.001720 0.000190 0.002562 0.000566 0.001598 0.000080 0.003621

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.86 -2.01 -5.99 -2.12 -4.45 0.29 -3.10 1.90 0.71 4.90 -1.88 -1.61 -5.67 -0.85 -3.31
Bud Neck -2.22 -6.00 -8.93 -2.28 -0.65 -4.93 -10.73 -6.29 -2.83 3.58 -5.71 -7.45 -10.24 -3.97 -0.08
Bud Site -5.42 -2.56 -7.45 -0.77 -5.22 -3.87 -3.78 -5.31 -2.70 0.32 -6.20 -3.45 -9.21 -3.46 -5.54
Cell Periphery -5.03 0.07 -6.74 -0.52 -6.75 2.46 1.87 2.37 -4.31 10.46 2.26 2.22 1.56 -5.83 -6.11
Cytoplasm 2.97 32.63 23.94 6.32 0.85 -0.25 18.87 4.28 5.62 -17.40 6.56 33.73 22.17 11.33 -9.19
Cytoplasmic Foci -4.13 18.77 10.41 6.63 -13.07 2.79 8.80 11.25 11.21 2.52 -1.55 22.66 14.70 13.22 -14.44
Eisosomes -4.06 -0.72 -9.91 2.05 -9.86 2.35 2.26 2.36 4.70 13.87 2.30 2.31 2.31 2.49 1.38
Endoplasmic Reticulum -0.54 2.17 2.09 2.06 -0.40 6.75 1.84 7.86 6.41 12.65 3.86 4.17 5.60 5.43 8.97
Endosome -2.18 4.14 -3.85 0.48 -5.23 -0.56 -0.85 3.91 4.38 5.99 -4.44 4.98 -1.80 3.04 -5.20
Golgi 2.56 3.85 -2.77 -3.37 -3.69 -3.49 3.28 -1.24 2.10 -1.89 -3.23 4.38 -2.79 1.14 -4.13
Lipid Particles -2.95 -2.54 -8.02 -4.49 -7.62 1.70 0.55 1.32 -2.68 5.34 0.89 1.12 -1.59 -4.91 -8.04
Mitochondria 2.19 0.37 -1.84 -3.24 -2.26 -2.40 -1.99 -2.33 2.18 -1.17 -1.69 0.07 -1.44 0.93 -1.86
Mitotic Spindle 0.20 -4.53 -5.71 -5.95 -3.48 -5.41 -2.24 -6.26 -0.51 -3.87 -6.05 -4.83 -8.76 0.16 -6.14
None 4.97 -4.73 4.64 -2.13 15.47 2.38 2.23 2.42 0.32 0.89 4.62 -1.12 4.49 -0.62 17.30
Nuclear Periphery -2.31 -19.02 -9.24 -5.48 2.97 -0.01 -15.32 -4.67 -5.73 12.95 -4.76 -22.87 -11.13 -8.78 8.91
Nuclear Periphery Foci -4.88 5.85 -3.96 3.90 -8.26 5.96 -0.01 6.38 2.02 9.62 3.21 5.12 4.56 1.56 -1.40
Nucleolus 3.38 -0.06 -1.98 -4.28 -2.16 -1.67 0.17 -0.44 1.54 -0.71 -1.86 0.28 -2.05 1.40 -2.59
Nucleus 4.60 -41.03 -9.32 -9.33 26.11 2.56 -9.15 -0.01 -3.51 9.77 5.68 -41.78 -5.84 -11.02 35.44
Peroxisomes -1.83 3.73 -1.95 -1.07 -2.13 0.70 1.08 0.95 2.11 -2.25 -0.44 1.28 -0.20 0.39 -2.90
Vacuole -3.78 -10.39 -25.48 -6.88 -19.88 -3.22 -17.06 -13.46 -11.47 12.29 -10.58 -15.42 -30.91 -16.73 -13.65
Vacuole Periphery 3.40 4.57 -2.90 -3.59 -3.79 -0.66 1.70 0.05 0.86 -2.37 -1.95 3.41 -2.36 0.23 -4.54
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR287C

YLR287C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr287c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available