Standard name
Human Ortholog
Description Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud 0.05 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Cytoplasm 0.18 0.18 0.44 0.31 0.26 0.12 0.12 – 0.07 0.06 0 0 0.38 0.72 0.66 0.32 0.22 0.31 0 0 0 0.05 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0.11 0.12 0.1 0.18 0.06 0.1 – 0 0 0 0 0.11 0.12 0.14 0.14 0.24 0.21 0.08 0.09 0.07 0.05 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Mitochondria 0.79 0.54 0.13 0.41 0.36 0.73 0.74 – 0.85 0.86 0.99 0.93 0.24 0 0 0.07 0.08 0.12 0.71 0.7 0.69 0.66 0.67 0.7
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.11 0.18 0 0 – 0 0 0 0 0 0 0.05 0.12 0.13 0.07 0 0 0 0 0 0
SpindlePole 0.05 0.08 0.16 0.13 0.14 0.09 0.13 – 0.08 0.08 0 0.09 0.08 0.05 0 0.16 0.13 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.14 0.1 0 0.12 0.05 0.07 – 0.09 0.06 0 0 0.11 0 0.1 0.1 0.18 0.18 0 0.06 0.05 0.09 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 7 2 0 1 2 1 – 0 1 1 2 3 6 0 5 0 0 5 12 3 1 4 4
Bud 14 7 2 3 3 3 17 – 2 4 7 9 1 4 0 8 3 0 1 2 1 0 2 2
Bud Neck 1 0 2 3 0 0 0 – 0 2 0 0 0 1 1 0 1 0 3 4 3 1 2 4
Bud Site 4 1 0 0 0 2 2 – 1 5 2 4 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 12 3 4 3 2 2 – 1 1 1 3 2 4 3 8 6 4 0 0 2 0 0 0
Cytoplasm 50 56 51 54 57 28 42 – 19 16 4 11 25 127 90 53 26 22 6 3 6 6 20 11
Endoplasmic Reticulum 1 11 4 3 4 5 4 – 1 5 3 2 5 6 4 10 7 4 0 6 1 1 3 2
Endosome 6 33 14 18 39 14 33 – 3 5 0 3 7 21 19 23 29 15 25 43 16 5 6 9
Golgi 0 2 0 1 2 2 1 – 1 1 0 0 1 1 0 9 5 5 3 13 2 0 1 3
Mitochondria 222 167 15 72 79 169 252 – 219 235 245 243 16 3 1 11 10 9 228 323 166 84 177 184
Nucleus 1 2 2 0 1 4 7 – 6 10 2 5 0 1 2 2 3 0 0 1 0 1 1 0
Nuclear Periphery 0 1 0 0 0 1 1 – 3 3 2 1 0 0 0 0 1 0 1 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 3 – 1 1 0 2 0 0 1 1 1 1 2 1 0 0 1 2
Peroxisomes 2 12 9 19 39 6 11 – 3 5 1 5 2 7 7 20 15 5 11 11 6 1 3 7
SpindlePole 13 26 19 23 32 21 43 – 21 23 6 23 5 8 3 26 15 9 10 5 8 3 7 7
Vac/Vac Membrane 18 45 12 4 27 12 24 – 22 16 9 10 7 5 14 16 21 13 13 27 12 11 23 17
Unique Cell Count 282 312 116 175 221 230 340 258 274 248 262 66 176 137 167 119 72 322 465 240 128 264 262
Labelled Cell Count 332 382 135 204 287 271 443 303 333 283 323 74 194 145 193 143 87 322 465 240 128 264 262


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.9 5.4 5.1 5.8 4.6 4.9 – 4.2 4.4 3.8 4.1 5.6 5.5 5.4 7.4 7.7 7.1 5.1 5.1 5.2
Std Deviation (1e-4) 0.6 0.9 1.3 1.4 2.1 1.5 1.6 – 1.0 0.8 0.6 0.6 2.1 1.2 1.3 2.8 3.2 2.6 1.2 1.0 1.3
Intensity Change (Log2) – – – -0.08 0.09 -0.23 -0.16 – -0.37 -0.3 -0.51 -0.41 0.05 0.03 0.0 0.44 0.52 0.39 -0.1 -0.09 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.2 1.6 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.056 -0.6857 -0.552 -0.9392 -1.0776 -0.7273 0.7854 0.3411 0.4777 0.206 1.2161 0.3536 0.6409 -0.2383 0.087 0.0032 -0.2085 -0.3464
Actin 0.1097 0.0208 0.0054 0.0088 0.0025 0.0174 0.0417 0.0069 0.0044 0.0376 0.0373 0.0127 0.075 0.0195 0.0535 0.0861 0.0909 0.0147
Bud 0.0024 0.0037 0.0069 0.0055 0.0016 0.0033 0.0014 0.0045 0.002 0.0034 0.0016 0.0019 0.0033 0.0041 0.003 0.0052 0.0029 0.0014
Bud Neck 0.0119 0.0027 0.006 0.0218 0.0027 0.0235 0.0023 0.0014 0.0041 0.001 0.0293 0.006 0.0112 0.0013 0.0064 0.0196 0.0022 0.0083
Bud Periphery 0.0051 0.0072 0.0067 0.0181 0.0031 0.0047 0.0032 0.0106 0.0065 0.0075 0.0038 0.0071 0.0046 0.0044 0.0087 0.009 0.006 0.0048
Bud Site 0.0317 0.0473 0.0167 0.0127 0.0094 0.0018 0.0075 0.025 0.0154 0.0043 0.0072 0.0023 0.0218 0.0298 0.0132 0.0122 0.0199 0.0033
Cell Periphery 0.003 0.0033 0.0022 0.0084 0.0007 0.0018 0.0031 0.0028 0.0018 0.0006 0.0008 0.0012 0.0013 0.0019 0.0046 0.0038 0.0007 0.0015
Cytoplasm 0.0317 0.0896 0.0731 0.0478 0.0328 0.066 0.0425 0.0689 0.06 0.0248 0.0194 0.0668 0.0394 0.0802 0.1355 0.1917 0.1993 0.1308
Cytoplasmic Foci 0.0931 0.1352 0.087 0.0874 0.0965 0.1494 0.1056 0.1171 0.1053 0.1214 0.1229 0.1182 0.13 0.1145 0.1062 0.0923 0.1371 0.1634
Eisosomes 0.0012 0.0018 0.0011 0.0044 0.0004 0.0014 0.0031 0.0009 0.0009 0.0006 0.0003 0.0007 0.0014 0.0007 0.0016 0.0006 0.0004 0.0002
Endoplasmic Reticulum 0.0139 0.0069 0.0103 0.006 0.0024 0.0087 0.008 0.0062 0.0045 0.0026 0.0013 0.0037 0.0244 0.0141 0.0079 0.019 0.005 0.0072
Endosome 0.2032 0.1287 0.1158 0.135 0.0837 0.0852 0.1099 0.1244 0.0807 0.1413 0.1163 0.0917 0.1421 0.1394 0.1346 0.2029 0.1834 0.2219
Golgi 0.0481 0.0265 0.0267 0.0227 0.0413 0.0218 0.0318 0.0327 0.0199 0.0316 0.0379 0.0236 0.0706 0.0565 0.0715 0.0843 0.0585 0.0744
Lipid Particles 0.0705 0.0316 0.0396 0.01 0.0339 0.0497 0.0567 0.0227 0.0233 0.0384 0.0757 0.0321 0.0857 0.0224 0.0182 0.0232 0.0149 0.0146
Mitochondria 0.1942 0.181 0.2709 0.373 0.3349 0.2484 0.1738 0.1883 0.2692 0.3865 0.2384 0.3113 0.1349 0.2284 0.2208 0.0928 0.1429 0.1728
None 0.0668 0.2334 0.2489 0.1403 0.3174 0.2237 0.2868 0.31 0.3202 0.0868 0.1972 0.2266 0.1119 0.2339 0.1485 0.0879 0.0528 0.1077
Nuclear Periphery 0.0078 0.0024 0.0029 0.0117 0.0007 0.0019 0.012 0.0022 0.0035 0.0051 0.0015 0.0033 0.0333 0.0024 0.0039 0.0034 0.0014 0.0063
Nucleolus 0.0027 0.0024 0.0025 0.0025 0.0009 0.0017 0.0069 0.0028 0.0047 0.0031 0.0039 0.0021 0.0026 0.0009 0.0011 0.0015 0.0004 0.0013
Nucleus 0.0045 0.0028 0.0037 0.0063 0.0009 0.0029 0.0181 0.0042 0.0048 0.0045 0.0021 0.0035 0.0321 0.0018 0.0023 0.0037 0.001 0.0059
Peroxisomes 0.0394 0.0396 0.0477 0.0521 0.0231 0.0695 0.0521 0.0453 0.046 0.0627 0.0831 0.0648 0.0433 0.0255 0.0244 0.0116 0.0517 0.0393
Punctate Nuclear 0.0102 0.0021 0.002 0.0017 0.0013 0.0034 0.0155 0.003 0.006 0.0018 0.0035 0.0084 0.0146 0.001 0.0064 0.0013 0.0016 0.0054
Vacuole 0.0391 0.0253 0.0183 0.015 0.0073 0.0095 0.0142 0.0159 0.0131 0.0225 0.0118 0.0079 0.0109 0.0137 0.0189 0.0394 0.0114 0.0097
Vacuole Periphery 0.0098 0.0057 0.0056 0.0088 0.0025 0.0043 0.0037 0.0043 0.0038 0.0118 0.0048 0.004 0.0058 0.0037 0.0088 0.0085 0.0155 0.0048

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4088 3.5628 4.1544 4.0665 5.5364 3.9833 7.5906 7.2939 4.7139 6.8409
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
925 1599 480 1224 1947 2396 241 47 2872 3995 721 1271

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 561.70 670.54 849.00 801.95 675.97 687.14 850.09 830.12 639.17 680.50 849.36 802.99
Standard Deviation 69.08 84.44 92.22 106.55 80.07 84.46 79.28 99.22 93.45 84.85 88.11 106.42
Intensity Change Log 2 — 0.255524 0.595965 0.513712 — 0.023645 0.330656 0.296361 — 0.133517 0.457136 0.399076

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000475 0.000876 0.003073 0.009488 0.000630 0.001001 0.001642 0.008198 0.000580 0.000951 0.002595 0.009441
Bud Neck 0.040862 0.026063 0.027966 0.038670 0.023283 0.025148 0.028350 0.016328 0.028944 0.025514 0.028094 0.037844
Bud Site 0.012154 0.022398 0.045674 0.098835 0.011353 0.016281 0.018894 0.071210 0.011611 0.018730 0.036723 0.097813
Cell Periphery 0.001431 0.000188 0.000243 0.000463 0.000246 0.000140 0.000234 0.000267 0.000628 0.000159 0.000240 0.000456
Cytoplasm 0.081254 0.043212 0.106129* 0.118718* 0.055388 0.033342 0.157321* 0.073325 0.063719 0.037292 0.123241* 0.117040*
Cytoplasmic Foci 0.175040 0.180598* 0.117769 0.162220 0.247322* 0.172918 0.199999* 0.240480* 0.224042* 0.175992 0.145255 0.165114
Eisosomes 0.000415 0.000144 0.000145 0.000176 0.000197 0.000141 0.000230 0.000228 0.000267 0.000142 0.000173 0.000178
Endoplasmic Reticulum 0.003546 0.000725 0.015426 0.003384 0.001754 0.000527 0.005440 0.003446 0.002331 0.000606 0.012088 0.003386
Endosome 0.032461 0.071079 0.044880 0.041996 0.056123 0.072102 0.062607 0.049707 0.048502 0.071693 0.050805 0.042281
Golgi 0.049230 0.092779 0.016665 0.033928 0.085230 0.102168 0.012327 0.050771 0.073635 0.098410 0.015215 0.034551
Lipid Particles 0.045552 0.010001 0.012759 0.004078 0.026662 0.008796 0.028880 0.006872 0.032746 0.009279 0.018148 0.004182
Mitochondria 0.442335* 0.397624* 0.032600 0.084679 0.347064* 0.431076* 0.013957 0.025657 0.377749* 0.417687* 0.026368 0.082497
Mitotic Spindle 0.001047 0.021474 0.100744* 0.089770 0.012625 0.022067 0.040033 0.099205 0.008896 0.021829 0.080451 0.090119
None 0.008713 0.002165 0.001781 0.001675 0.001588 0.000843 0.003222 0.000356 0.003883 0.001372 0.002262 0.001626
Nuclear Periphery 0.000431 0.000297 0.005567 0.000717 0.000581 0.000201 0.003072 0.000436 0.000533 0.000239 0.004733 0.000706
Nuclear Periphery Foci 0.000276 0.000408 0.006286 0.000921 0.000385 0.000212 0.003188 0.001306 0.000350 0.000291 0.005250 0.000936
Nucleolus 0.003933 0.002619 0.009291 0.001454 0.002841 0.001591 0.011766 0.001607 0.003193 0.002002 0.010118 0.001460
Nucleus 0.027755 0.013525 0.266430* 0.035435 0.023062 0.007684 0.195873* 0.043302 0.024573 0.010022 0.242846* 0.035726
Peroxisomes 0.039488 0.073334 0.054870 0.149040* 0.061337 0.057385 0.072642 0.225688* 0.054300 0.063769 0.060810 0.151875*
Vacuole 0.018962 0.029083 0.120546 0.117165 0.030464 0.034566 0.133404 0.078516 0.026759 0.032372 0.124844 0.115736
Vacuole Periphery 0.014641 0.011408 0.011155 0.007186 0.011866 0.011811 0.006919 0.003094 0.012760 0.011649 0.009739 0.007035

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.80 -8.56 -10.32 -9.92 -6.49 -2.54 -4.89 -3.65 -3.45 -3.01 -3.12 -10.39 -10.71 -10.17 -7.49
Bud Neck 3.66 2.60 0.67 -4.20 -2.62 -0.87 -0.78 1.78 2.26 1.97 1.68 0.09 -3.25 -4.88 -3.02
Bud Site -4.96 -6.84 -17.25 -14.96 -7.47 -3.91 -2.86 -3.04 -2.77 -2.64 -6.49 -7.55 -18.32 -16.54 -10.34
Cell Periphery 5.15 4.89 3.61 -1.34 -0.95 3.20 0.42 0.10 -1.33 -0.11 5.74 4.66 1.38 -1.55 -0.99
Cytoplasm 6.52 -2.93 -6.77 -14.64 -2.75 6.77 -7.54 -0.94 -2.26 3.89 9.02 -8.59 -11.37 -17.06 -0.67
Cytoplasmic Foci -0.85 7.26 3.02 4.62 -5.31 12.80 4.42 0.73 -2.24 -1.29 10.26 12.35 12.02 4.11 -2.19
Eisosomes 9.26 8.67 8.30 -2.84 -1.31 5.22 -2.68 -0.71 -2.16 0.46 10.46 6.08 7.30 -3.67 1.20
Endoplasmic Reticulum 4.69 -9.14 -0.16 -5.39 9.20 6.06 -6.26 -1.78 -2.85 1.30 7.23 -11.25 -2.38 -5.91 8.85
Endosome -11.26 -2.46 -1.63 9.68 1.24 -5.09 -0.44 0.48 2.01 0.65 -9.67 0.33 4.35 12.71 2.86
Golgi -8.78 8.91 5.47 15.56 -4.60 -3.96 16.12 2.56 3.54 -1.60 -7.40 20.02 14.02 22.28 -5.53
Lipid Particles 10.34 8.76 12.52 5.80 4.90 9.94 -0.11 4.22 -0.31 3.28 14.18 6.47 17.90 7.10 7.47
Mitochondria 3.31 34.21* 29.70* 33.11* -7.42 -8.54 41.85* 28.35* 37.28* -0.85 -4.84 52.00* 39.60* 49.94* -9.03
Mitotic Spindle -11.80 -10.90 -16.53 -11.88 0.89 -4.79 -3.60 -2.41 -2.12 -1.42 -9.02 -10.56 -14.95 -12.32 -1.04
None 3.37 3.75 3.88 1.08 0.80 2.59 -2.18 4.60 2.52 4.21 3.75 2.37 3.30 -0.12 1.73
Nuclear Periphery 1.50 -7.33 -1.94 -2.50 6.32 6.21 -4.39 -0.01 -1.84 4.14 5.76 -8.33 -1.54 -2.86 6.96
Nuclear Periphery Foci -1.12 -5.58 -5.97 -3.86 4.80 4.29 -6.13 -2.93 -3.23 1.80 1.02 -6.70 -5.90 -5.98 5.58
Nucleolus 1.37 -4.45 3.53 2.14 6.95 2.42 -4.84 1.33 -0.41 5.22 2.50 -6.96 3.94 1.41 8.84
Nucleus 4.12 -17.84 -5.06 -10.11 16.25 8.69 -11.40 -1.36 -2.20 6.09 8.86 -21.27 -7.15 -12.30 18.40
Peroxisomes -7.70 -1.88 -14.32 -8.46 -10.08 1.16 -1.13 -3.80 -3.93 -3.42 -3.30 -0.64 -12.96 -11.37 -10.32
Vacuole -4.66 -15.74 -22.90 -21.12 -3.02 -1.86 -10.44 -3.53 -3.39 0.04 -3.50 -18.18 -22.23 -21.18 -2.74
Vacuole Periphery 2.34 2.61 6.03 4.86 2.14 -0.02 4.44 7.60 8.13 2.25 1.38 3.48 6.85 6.34 1.92
Standard name
Human Ortholog
Description Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
Localization
Cell Percentages mitochondrion (79%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Guf1

Guf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Guf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available