Standard name
Human Ortholog
Description Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.14 0.17 0.13 0.1 0.08 0.08 – 0.11 0.09 0.11 0.05 0.06 0 0.08 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0.06 0.06 0.11 0.14 0.09 0.08 0.06 0.05 – 0.08 0.12 0.09 0.12 0.14 0.21 0.58 – – 0 0 0 0 0 0
Cytoplasm 0.07 0.13 0.08 0.14 0.13 0.17 0.17 0.14 – 0.14 0.22 0.18 0.09 0.13 0.13 0.33 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0.9 0.85 0.88 0.66 0.74 0.67 0.55 0.64 – 0.5 0.35 0.51 0.85 0.85 0.8 0 – – 0.94 0.91 0.91 0.88 0.89 0.81
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.19 0.16 0.17 0.26 0.19 – 0.21 0.28 0.31 0 0 0 0.08 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0.08 0 0.07 0.13 0.07 – 0.19 0.16 0.09 0 0 0 0 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 10 22 46 48 43 49 44 – 62 42 65 12 23 17 2 – – 2 2 5 2 2 7
Bud 0 0 1 4 3 9 16 11 – 23 33 24 0 0 0 0 – – 2 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 24 15 18 37 31 33 37 32 – 45 60 52 32 55 88 14 – – 2 4 3 1 4 7
Cytoplasm 28 35 13 38 48 69 100 83 – 78 106 103 24 49 53 8 – – 5 6 1 5 3 5
Endoplasmic Reticulum 348 226 140 178 267 275 327 372 – 284 172 292 223 326 327 1 – – 382 255 149 177 210 232
Endosome 0 0 0 2 3 1 10 2 – 4 1 0 0 0 0 0 – – 0 0 0 0 0 2
Golgi 9 2 1 11 11 1 1 1 – 1 0 0 3 11 2 0 – – 3 1 0 1 3 7
Mitochondria 1 3 12 51 56 69 156 113 – 120 137 176 0 0 0 2 – – 1 0 0 1 2 7
Nucleus 0 0 0 0 0 4 5 1 – 2 2 3 0 1 0 0 – – 1 0 0 0 0 0
Nuclear Periphery 0 0 2 2 3 3 4 11 – 16 9 8 0 1 1 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 – 0 4 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 26 1 2 22 11 27 80 41 – 107 79 49 5 4 12 0 – – 1 3 1 7 4 9
Unique Cell Count 386 265 159 270 360 412 598 584 569 493 572 263 382 410 24 405 280 164 202 238 287
Labelled Cell Count 445 292 211 391 481 534 785 712 742 645 772 299 470 500 27 405 280 164 202 238 287


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.1 18.0 11.9 11.2 11.0 10.4 9.7 9.8 – 9.3 9.7 9.3 15.2 15.9 15.8 16.4 – – 17.3 17.3 17.5
Std Deviation (1e-4) 2.7 3.1 2.1 1.8 1.9 1.9 1.8 1.7 – 1.9 1.9 2.1 2.5 2.7 2.7 2.7 – – 3.1 3.8 3.9
Intensity Change (Log2) – – – -0.09 -0.12 -0.19 -0.29 -0.28 – -0.35 -0.3 -0.35 0.36 0.42 0.42 0.47 – – 0.54 0.54 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 -0.2 -1.1 -2.2 -2.5 – -1.0 -2.0 -0.9 -3.4 -3.0 -4.1 0 – –
Bud 0 0 0 0 0 – 2.1 3.0 2.2 0 0 0 0 – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Cell Periphery 0.7 -1.0 -1.2 -2.2 -2.6 – -1.4 0.3 -0.8 0.3 1.0 2.8 0 – –
Cytoplasm 1.8 1.7 2.6 2.7 2.0 – 1.9 3.8 3.0 0.3 1.5 1.6 0 – –
Endoplasmic Reticulum -5.1 -3.5 -5.1 -7.7 -5.9 – -8.6 -11.7 -8.4 -0.9 -0.8 -2.3 -9.1 – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Mitochondria 3.2 2.5 2.8 5.0 3.5 – 3.9 5.3 5.9 0 0 0 0 – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 – –
Vacuole 3.0 0 2.6 4.4 2.8 – 5.5 4.9 3.2 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.395 11.5807 10.7165 9.9749 9.2959 10.8994 12.2441 13.9698 12.884 12.8571 10.7663 12.46 12.2449 12.2344 11.9591 10.4139 11.384 11.6436
Actin 0.0065 0.0031 0.0127 0.011 0.0033 0.018 0.0306 0.0025 0.0103 0 0.0018 0.0028 0.0004 0.0003 0.0012 0.0001 0.0041 0.0016
Bud 0.0002 0.0001 0.0005 0.0002 0.0003 0.0001 0.0001 0.0012 0.0007 0 0.0001 0.0001 0 0 0 0 0 0
Bud Neck 0.0003 0.0006 0.0012 0.0146 0.0001 0.0005 0.0002 0.0002 0.0006 0 0.0003 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0008
Bud Periphery 0.0006 0.0002 0.0007 0.0004 0.0005 0.0003 0.0003 0.008 0.001 0 0.0004 0.0002 0.0001 0 0 0 0.0001 0.0003
Bud Site 0.0005 0.0021 0.0064 0.0002 0.0007 0.0001 0.0004 0.0015 0.0044 0 0.0008 0.0001 0 0.0001 0 0 0.0001 0
Cell Periphery 0.0018 0.0068 0.0018 0.0041 0.0005 0.0004 0.0015 0.0083 0.0019 0.0004 0.001 0.0009 0.0013 0.0052 0.0019 0.0005 0.0006 0.002
Cytoplasm 0.0282 0.059 0.0402 0.0179 0.0128 0.0113 0.0127 0.0032 0.0067 0.0019 0.0021 0.0145 0.0041 0.0158 0.0048 0.0078 0.0014 0.0046
Cytoplasmic Foci 0.0095 0.0022 0.0101 0.005 0.0174 0.0011 0.0034 0.0002 0.0017 0 0.0019 0.0042 0.0007 0.0006 0.0002 0.0005 0.002 0.0001
Eisosomes 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0005 0.0001 0 0.0003 0 0 0.0001 0 0 0.0003 0
Endoplasmic Reticulum 0.8499 0.8857 0.8496 0.828 0.8537 0.9104 0.8905 0.9345 0.9445 0.9917 0.8705 0.9409 0.9585 0.9488 0.9745 0.9834 0.8943 0.9764
Endosome 0.0346 0.0044 0.0202 0.043 0.0335 0.0103 0.0123 0.0015 0.0063 0.0007 0.0185 0.0097 0.0049 0.002 0.001 0.0024 0.0114 0.0018
Golgi 0.0147 0.0038 0.009 0.0252 0.005 0.0048 0.0076 0.0006 0.0055 0.0001 0.0038 0.0069 0.0007 0.0017 0.0076 0.0006 0.0118 0.0011
Lipid Particles 0.015 0.018 0.0106 0.0164 0.0081 0.0051 0.0041 0.0057 0.0025 0.0001 0.0153 0.0079 0.0021 0.0139 0.0031 0.0005 0.0095 0.001
Mitochondria 0.008 0.0003 0.0083 0.0018 0.0104 0.0036 0.0026 0.0127 0.0011 0.0001 0.0179 0.0021 0.0004 0.0001 0.0001 0 0.0068 0.0016
None 0.0014 0.0003 0.0035 0.0004 0.0228 0.0004 0.0076 0.0001 0.0009 0 0.0036 0.0003 0.0015 0.0001 0 0 0.0004 0.0001
Nuclear Periphery 0.0159 0.0075 0.006 0.0251 0.0104 0.0235 0.014 0.0058 0.0046 0.0038 0.034 0.0037 0.0201 0.008 0.004 0.0034 0.0251 0.0064
Nucleolus 0.0002 0 0.0009 0.0001 0.0011 0.0001 0.0001 0.0001 0.0004 0 0.0047 0 0 0 0 0 0.0001 0
Nucleus 0.0019 0.0006 0.0039 0.001 0.0046 0.0043 0.0019 0.0005 0.0023 0.0001 0.0079 0.0004 0.0012 0.0004 0.0001 0.0001 0.0007 0.0003
Peroxisomes 0.0018 0 0.0036 0.0003 0.0022 0.0011 0.0036 0 0.0003 0 0.0011 0.0019 0.0001 0 0 0 0.0274 0
Punctate Nuclear 0.0035 0.0002 0.0016 0.0015 0.0086 0.0011 0.0024 0 0.0018 0 0.0041 0.0002 0.0001 0.0001 0 0 0.0004 0
Vacuole 0.004 0.0049 0.0076 0.0027 0.0025 0.0021 0.0027 0.0056 0.0021 0.0006 0.0048 0.0019 0.0029 0.0025 0.0011 0.0005 0.0009 0.001
Vacuole Periphery 0.0012 0.0003 0.0013 0.0012 0.0013 0.0012 0.0011 0.0072 0.0004 0.0004 0.005 0.0003 0.0009 0.0002 0.0002 0.0002 0.0025 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.3039 44.0649 54.0776 56.4639 78.6591 68.214 56.1209 45.6847 51.3164 70.4555
Translational Efficiency 2.5793 2.7197 2.0617 2.367 1.7247 2.3879 1.8094 2.3876 2.0159 2.0088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
908 1246 128 887 1862 1463 887 1755 2770 2709 1015 2642

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 916.39 1464.57 1837.77 1753.89 1177.56 1370.03 1667.33 1668.39 1091.95 1413.51 1688.82 1697.09
Standard Deviation 168.48 265.29 238.87 272.51 213.80 221.46 243.08 297.46 234.65 247.14 249.07 292.12
Intensity Change Log 2 — 0.676444 1.003923 0.936525 — 0.218407 0.501739 0.502656 — 0.436918 0.743229 0.708731

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009372 0.001445 0.000418 0.001425 0.000687 0.000852 0.000354 0.003167 0.003534 0.001125 0.000362 0.002582
Bud Neck 0.000607 0.000347 0.001858 0.001315 0.000200 0.000244 0.000519 0.002974 0.000333 0.000291 0.000687 0.002417
Bud Site 0.015515 0.006005 0.001521 0.010991 0.003481 0.002050 0.001698 0.024951 0.007426 0.003869 0.001676 0.020264
Cell Periphery 0.018181 0.005355 0.014618 0.027235 0.005741 0.003225 0.005997 0.014792 0.009819 0.004204 0.007084 0.018970
Cytoplasm 0.013722 0.005550 0.000398 0.004630 0.009854 0.004631 0.002408 0.024868 0.011122 0.005054 0.002154 0.018073
Cytoplasmic Foci 0.017514 0.005163 0.000112 0.001166 0.017909 0.003464 0.000179 0.024635 0.017779 0.004246 0.000171 0.016756
Eisosomes 0.001080 0.000202 0.000662 0.000309 0.000149 0.000096 0.000086 0.000236 0.000454 0.000145 0.000159 0.000261
Endoplasmic Reticulum 0.856135* 0.946446* 0.953374* 0.905791* 0.919745* 0.964532* 0.976423* 0.746131* 0.898894* 0.956213* 0.973516* 0.799734*
Endosome 0.010765 0.007306 0.006524 0.004537 0.009749 0.004095 0.002940 0.026514 0.010082 0.005572 0.003392 0.019136
Golgi 0.012345 0.004246 0.002281 0.003893 0.006553 0.003503 0.000891 0.019327 0.008452 0.003845 0.001066 0.014145
Lipid Particles 0.012362 0.004055 0.004700 0.025627 0.009473 0.005550 0.004781 0.056496 0.010420 0.004862 0.004771 0.046133
Mitochondria 0.011544 0.001915 0.000059 0.000412 0.002391 0.001202 0.000069 0.003136 0.005391 0.001530 0.000068 0.002221
Mitotic Spindle 0.000169 0.000413 0.000051 0.000299 0.000137 0.000058 0.000024 0.005225 0.000147 0.000221 0.000028 0.003571
None 0.005369 0.001207 0.000008 0.000675 0.000891 0.002188 0.000119 0.000922 0.002359 0.001737 0.000105 0.000839
Nuclear Periphery 0.000139 0.000309 0.001039 0.000923 0.000281 0.000105 0.000203 0.001011 0.000235 0.000199 0.000308 0.000981
Nuclear Periphery Foci 0.001958 0.003071 0.005444 0.002092 0.003951 0.000628 0.000858 0.017196 0.003298 0.001751 0.001437 0.012125
Nucleolus 0.000035 0.000025 0.000002 0.000010 0.000017 0.000050 0.000002 0.000057 0.000023 0.000038 0.000002 0.000041
Nucleus 0.000374 0.000454 0.000053 0.000173 0.000180 0.000569 0.000104 0.001056 0.000244 0.000516 0.000098 0.000760
Peroxisomes 0.008938 0.001354 0.000009 0.000446 0.003351 0.001506 0.000142 0.001391 0.005182 0.001436 0.000125 0.001074
Vacuole 0.002180 0.002867 0.006285 0.007112 0.002877 0.000924 0.002037 0.021621 0.002649 0.001818 0.002573 0.016750
Vacuole Periphery 0.001697 0.002263 0.000584 0.000939 0.002381 0.000529 0.000167 0.004295 0.002157 0.001327 0.000219 0.003168

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.41 6.23 5.39 -0.02 -2.64 -0.46 1.74 -5.88 -4.51 -6.79 4.46 6.40 1.59 -3.97 -7.26
Bud Neck 1.89 -2.04 -2.58 -3.71 0.80 -0.80 -1.84 -8.61 -8.32 -6.76 0.56 -2.05 -8.94 -9.04 -6.11
Bud Site 3.70 5.91 1.32 -2.01 -4.07 2.11 2.76 -9.28 -9.77 -9.94 3.52 6.24 -6.96 -9.15 -10.59
Cell Periphery 4.92 0.79 -2.62 -8.73 -2.82 2.70 -0.23 -5.79 -7.75 -5.35 5.26 2.06 -5.87 -11.30 -7.78
Cytoplasm 4.33 7.64 4.21 0.58 -3.35 4.92 6.81 -7.50 -10.59 -11.58 6.36 9.14 -4.81 -9.53 -11.38
Cytoplasmic Foci 5.28 8.07 7.48 4.12 -3.03 8.05 10.56 -2.92 -11.49 -14.05 9.42 13.22 0.43 -9.70 -14.03
Eisosomes 4.98 0.72 4.22 -1.20 0.63 2.02 2.19 -2.13 -3.70 -3.79 4.89 3.21 2.91 -2.72 -1.29
Endoplasmic Reticulum -9.22 -8.10 -4.48 5.19 4.56 -8.35 -11.34 18.93 24.76 26.67* -11.69 -15.58 13.89 23.67 26.52
Endosome 2.92 3.03 6.20 3.33 1.80 6.69 8.17 -11.20 -16.01 -16.90 6.38 9.65 -8.55 -13.57 -15.85
Golgi 5.00 7.10 5.58 0.28 -2.30 2.76 7.40 -7.18 -8.80 -11.42 5.12 10.73 -4.42 -8.27 -11.86
Lipid Particles 5.21 3.17 -3.85 -6.92 -5.80 2.85 3.23 -13.99 -15.16 -15.25 5.56 4.44 -14.14 -16.79 -16.05
Mitochondria 4.37 5.46 5.29 2.28 -3.77 1.96 4.29 -0.81 -2.39 -4.02 4.53 6.79 3.38 -1.14 -4.24
Mitotic Spindle -1.57 4.51 -1.38 0.54 -2.53 1.27 1.92 -6.26 -6.36 -6.40 -0.91 2.98 -6.31 -6.15 -6.53
None 2.34 3.12 2.67 0.88 -1.71 -2.08 4.86 -0.11 1.96 -3.49 0.90 3.92 2.50 2.05 -3.63
Nuclear Periphery -1.75 -3.45 -6.93 -4.14 0.43 3.32 1.52 -5.79 -7.51 -6.78 0.61 -1.51 -8.02 -7.96 -7.15
Nuclear Periphery Foci -1.29 -2.25 -0.22 0.95 2.04 4.78 4.45 -7.44 -9.76 -9.63 2.57 3.50 -7.27 -8.69 -9.15
Nucleolus 0.48 1.84 1.40 1.16 -2.56 -1.11 6.08 -3.82 -0.05 -5.41 -0.89 3.43 -1.98 -0.00 -5.71
Nucleus -0.27 4.62 2.57 0.93 -3.55 -1.19 1.56 -7.17 -1.42 -7.61 -1.24 3.33 -6.19 -1.14 -7.67
Peroxisomes 5.42 6.71 6.35 2.02 -3.75 3.23 7.17 3.88 0.28 -4.97 6.25 9.49 7.39 1.13 -5.45
Vacuole -0.70 -2.57 -4.05 -4.02 -0.56 4.07 1.28 -11.44 -12.90 -11.95 1.74 0.02 -12.06 -13.11 -12.09
Vacuole Periphery -0.73 3.34 2.51 1.72 -1.22 4.50 5.57 -2.78 -6.53 -7.29 1.86 6.90 -2.16 -3.58 -7.68
Standard name
Human Ortholog
Description Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec63p, Sec62p, and Sec66p
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Sec72

Sec72


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec72-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available