ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.72 0.62 0.79 0.83 0.6 0.59 0.56 0.49 0.58 0.62 0.63 0.61 0.77 0.81 0.88 0.14 0.13 0.16 0.29 0.13 0.2 0.16 0.22 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0.08 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0.09 0 0 0 0 0.07 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.08 0 0.15 0.23 0.07 0.17 0.19 0.16 0.22 0.17 0.17 0.2 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0.14 0.15 0.13 0.1 0.1 0.07 0 0 0 0.21 0.17 0.26 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.25 0.42 0.1 0.14 0.44 0.51 0.4 0.46 0.41 0.36 0.35 0.38 0.2 0.15 0.11 0.68 0.71 0.66 0.58 0.78 0.69 0.64 0.61 0.6
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud 0 2 1 0 1 1 0 1 1 3 1 4 3 1 1 0 0 0 6 7 0 2 9 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 231 239 41 122 107 114 193 191 147 201 178 235 208 304 342 20 20 18 104 52 16 17 64 66
Endoplasmic Reticulum 2 3 0 0 0 0 1 1 0 0 0 4 0 2 1 3 9 11 1 1 0 0 2 2
Endosome 3 5 0 0 14 1 2 1 0 2 0 0 19 35 18 3 14 3 13 11 0 7 7 15
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 2 4 6 2 1 0 1 1 3
Mitochondria 25 17 8 34 12 32 66 62 56 56 49 78 4 2 2 4 11 2 10 7 2 4 9 10
Nucleus 2 17 1 0 1 10 47 57 32 32 28 26 1 3 1 29 27 29 1 2 0 1 3 4
Nuclear Periphery 1 3 0 1 0 0 1 5 5 3 4 11 0 0 0 0 4 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 0 0 0 0 2 3 1 0 0 0 1 1 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 0 0 1 0 0 0 0 1 0 0 1 4 2 0 1 0 3 2 0 2 4 4
Vac/Vac Membrane 79 161 5 20 78 98 138 179 104 116 98 147 54 55 43 94 111 75 205 316 55 70 176 192
Unique Cell Count 321 386 52 147 177 193 343 386 253 326 282 385 270 376 390 139 156 113 357 408 81 111 289 319
Labelled Cell Count 344 450 57 178 215 256 449 498 345 416 361 506 291 409 412 156 202 145 357 408 81 111 289 319


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.6 4.0 3.6 5.1 4.4 4.2 4.5 4.1 4.6 4.6 4.7 4.7 4.9 4.9 10.5 9.5 9.0 4.3 5.4 5.4
Std Deviation (1e-4) 0.7 0.8 2.4 2.0 3.1 2.4 1.5 1.5 1.4 1.7 1.8 2.2 1.7 1.8 2.0 3.4 2.8 2.7 1.1 2.7 2.4
Intensity Change (Log2) – – – -0.15 0.36 0.15 0.07 0.17 0.04 0.21 0.2 0.23 0.25 0.3 0.29 1.41 1.26 1.18 0.1 0.43 0.44

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 7.1 7.7 6.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7242 0.1166 -0.1216 -0.1789 -0.4124 0.2827 -1.3587 -0.6916 -0.9631 -0.888 -0.988 -0.7228 1.0133 1.236 0.8849 1.0251 0.9458 0.9445
Actin 0.0059 0.0002 0.0267 0.0025 0.0091 0.0066 0.0251 0.0003 0.0011 0.0297 0.0004 0.0028 0.05 0.0001 0.0127 0.0213 0.008 0
Bud 0.0004 0.0003 0.0009 0.0004 0.0008 0.0005 0.0007 0.0023 0.0006 0.01 0.0003 0.0007 0.0015 0.0007 0.0009 0.0007 0.0005 0.0002
Bud Neck 0.0005 0.0009 0.0007 0.0072 0.0017 0.0017 0.0007 0.0049 0.0007 0.008 0.0005 0.0019 0.0153 0.0005 0.0031 0.0004 0.0006 0.0023
Bud Periphery 0.0012 0.0003 0.0013 0.0005 0.0012 0.0008 0.001 0.0031 0.0012 0.0362 0.0004 0.0011 0.0021 0.0007 0.0018 0.0011 0.0008 0.0004
Bud Site 0.0022 0.0014 0.008 0.0013 0.0037 0.0008 0.0047 0.0207 0.0024 0.0096 0.0008 0.0018 0.0143 0.0088 0.0099 0.0011 0.0026 0.0009
Cell Periphery 0.0008 0.0006 0.0006 0.0003 0.0011 0.0006 0.0005 0.001 0.0004 0.0017 0.0002 0.0004 0.0011 0.0007 0.0006 0.0002 0.0009 0.0014
Cytoplasm 0.1054 0.1289 0.1093 0.13 0.0512 0.1165 0.0923 0.1525 0.1181 0.1026 0.0959 0.1746 0.1185 0.1639 0.1185 0.0942 0.1371 0.0873
Cytoplasmic Foci 0.067 0.0749 0.052 0.0735 0.1204 0.0626 0.0665 0.0667 0.06 0.0581 0.0685 0.103 0.0684 0.0449 0.0593 0.0368 0.0551 0.0489
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002 0 0.0001 0.0006 0.0001 0.0001 0.0002 0.0002 0
Endoplasmic Reticulum 0.0084 0.0057 0.0078 0.0028 0.0023 0.0035 0.0039 0.0033 0.0043 0.0033 0.0022 0.0042 0.008 0.0067 0.0053 0.0018 0.0025 0.003
Endosome 0.1651 0.1668 0.1986 0.1917 0.3143 0.17 0.2377 0.1399 0.1765 0.2592 0.3524 0.2389 0.1989 0.1594 0.1717 0.2585 0.286 0.3222
Golgi 0.0055 0.0046 0.0085 0.0309 0.0106 0.0112 0.0125 0.0019 0.0056 0.0146 0.0102 0.0053 0.0076 0.0018 0.0061 0.0058 0.0048 0.0038
Lipid Particles 0.0236 0.0135 0.0166 0.0292 0.0525 0.0096 0.0196 0.0092 0.0141 0.0155 0.0041 0.0125 0.0183 0.0098 0.0065 0.0081 0.0156 0.0337
Mitochondria 0.0217 0.0019 0.0206 0.024 0.0104 0.0191 0.017 0.0029 0.028 0.0204 0.0243 0.0039 0.0052 0.0023 0.0058 0.0164 0.0051 0.0011
None 0.421 0.389 0.3282 0.2668 0.0428 0.2742 0.3825 0.4035 0.4539 0.2782 0.2513 0.2672 0.3503 0.3583 0.3867 0.314 0.2376 0.1067
Nuclear Periphery 0.0058 0.0061 0.0136 0.0023 0.0086 0.0034 0.0052 0.004 0.0083 0.0032 0.0404 0.0035 0.0108 0.0059 0.0055 0.0026 0.0055 0.0021
Nucleolus 0.0022 0.0015 0.0012 0.0015 0.0027 0.0015 0.0017 0.0028 0.0022 0.0011 0.0008 0.0018 0.0015 0.0025 0.0012 0.0011 0.0028 0.0037
Nucleus 0.0036 0.0034 0.0036 0.0036 0.0105 0.0032 0.0068 0.0071 0.0057 0.0025 0.0039 0.0057 0.0059 0.0039 0.0052 0.0022 0.008 0.0044
Peroxisomes 0.0111 0.0013 0.0032 0.0366 0.0159 0.0021 0.0082 0.0016 0.0119 0.0025 0.0011 0.0037 0.0112 0.0011 0.0122 0.0059 0.0085 0.0024
Punctate Nuclear 0.0091 0.0014 0.001 0.0024 0.0214 0.0013 0.004 0.0021 0.0014 0.0006 0.001 0.0024 0.0049 0.0009 0.005 0.0007 0.02 0.0006
Vacuole 0.1261 0.1861 0.1744 0.1706 0.294 0.277 0.0988 0.1603 0.0914 0.1131 0.1279 0.1529 0.0935 0.2079 0.1601 0.1966 0.1739 0.3453
Vacuole Periphery 0.0132 0.0113 0.0229 0.022 0.0249 0.0336 0.0104 0.0099 0.0121 0.0295 0.0135 0.0115 0.0122 0.0192 0.0217 0.0303 0.0239 0.0297

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
194 1416 1368 102 1767 1627 519 1492 1961 3043 1887 1594

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 715.45 769.85 825.46 1151.52 692.38 749.86 860.17 883.79 694.66 759.16 835.01 900.92
Standard Deviation 90.23 143.54 99.04 222.52 84.05 96.60 115.85 157.14 84.96 121.15 105.08 174.86
Intensity Change Log 2 — 0.105726 0.206347 0.686617 — 0.115057 0.313058 0.352140 — 0.110323 0.259815 0.531775

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000064 0.000143 0.000222 0.000133 0.000427 0.000235 0.000307 0.000157 0.000391 0.000192 0.000245 0.000155
Bud Neck 0.015364 0.026831 0.012586 0.014867 0.036255 0.037728 0.017178 0.020434 0.034189 0.032657 0.013849 0.020078
Bud Site 0.006522 0.020796 0.010020 0.019829 0.009833 0.021021 0.005795 0.026472 0.009505 0.020916 0.008858 0.026047
Cell Periphery 0.000176 0.000151 0.000129 0.000098 0.000168 0.000126 0.000105 0.000257 0.000169 0.000138 0.000122 0.000247
Cytoplasm 0.332359 0.150832 0.312473 0.040270 0.323591 0.236833 0.281293 0.114127 0.324458 0.196814 0.303898 0.109401
Cytoplasmic Foci 0.203936 0.092263 0.017735 0.029744 0.172680 0.126027 0.016608 0.041294 0.175773 0.110316 0.017425 0.040555
Eisosomes 0.000145 0.000057 0.000039 0.000016 0.000165 0.000077 0.000057 0.000048 0.000163 0.000067 0.000044 0.000046
Endoplasmic Reticulum 0.000715 0.001758 0.003217 0.000984 0.001380 0.001003 0.004845 0.002199 0.001314 0.001354 0.003664 0.002122
Endosome 0.059251 0.102240 0.011907 0.122908 0.061947 0.098413 0.022468 0.125890 0.061680 0.100194 0.014812 0.125699
Golgi 0.001079 0.004632 0.000184 0.027963 0.002701 0.004392 0.000217 0.007302 0.002541 0.004504 0.000193 0.008624
Lipid Particles 0.006760 0.004633 0.001750 0.000834 0.009967 0.005408 0.002620 0.004191 0.009649 0.005047 0.001989 0.003977
Mitochondria 0.000671 0.009495 0.000731 0.001919 0.005812 0.007786 0.000517 0.005367 0.005303 0.008581 0.000672 0.005147
Mitotic Spindle 0.000093 0.003927 0.010410 0.029227 0.002351 0.006546 0.002738 0.010565 0.002128 0.005327 0.008300 0.011759
None 0.000809 0.001464 0.004866 0.000084 0.001005 0.000945 0.005795 0.001914 0.000986 0.001187 0.005122 0.001796
Nuclear Periphery 0.000441 0.000414 0.002716 0.002849 0.000472 0.000347 0.002083 0.001151 0.000469 0.000378 0.002542 0.001260
Nuclear Periphery Foci 0.000503 0.000299 0.001538 0.005627 0.000289 0.000283 0.002705 0.003023 0.000311 0.000290 0.001859 0.003190
Nucleolus 0.001083 0.001279 0.002602 0.001694 0.000861 0.001197 0.003107 0.006525 0.000883 0.001235 0.002741 0.006216
Nucleus 0.078938 0.026633 0.526654 0.005650 0.075927 0.039209 0.496626 0.070200 0.076225 0.033357 0.518395 0.066069
Peroxisomes 0.003718 0.005690 0.002086 0.003921 0.005704 0.007989 0.007778 0.009369 0.005508 0.006919 0.003652 0.009020
Vacuole 0.282048 0.526755 0.077454 0.582640 0.285157 0.397088 0.126395 0.508962 0.284850 0.457426 0.090915 0.513677
Vacuole Periphery 0.005323 0.019707 0.000681 0.108742 0.003308 0.007348 0.000763 0.040552 0.003507 0.013099 0.000704 0.044916

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.60 -7.12 -1.40 0.29 3.11 0.57 0.09 0.80 0.62 5.19 0.67 0.20 0.77 0.43 4.73
Bud Neck -3.39 0.38 0.17 2.83 -0.09 -0.50 6.17 6.52 7.46 -1.59 0.68 8.75 6.28 7.00 -2.87
Bud Site -5.42 -1.20 -2.31 0.37 -1.83 -5.95 2.99 -7.68 -2.24 -9.41 -7.87 0.78 -8.04 -2.38 -8.20
Cell Periphery 1.25 2.25 3.31 2.92 1.88 2.00 3.30 -1.16 -1.88 -2.25 1.79 2.70 -1.05 -1.66 -1.94
Cytoplasm 9.49 2.41 11.08 5.10 12.82 9.32 6.49 22.64 12.61 9.56 16.82 5.98 24.48 10.32 18.80
Cytoplasmic Foci 8.02 13.70 11.74 8.90 -2.22 7.97 34.58 27.89 19.12 -9.43 13.40 38.11 30.01 20.82 -9.93
Eisosomes 5.53 6.22 7.96 8.71 8.43 8.51 9.14 11.72 6.27 3.58 10.40 12.24 12.77 5.84 2.16
Endoplasmic Reticulum -6.37 -13.53 -1.36 1.93 8.22 1.99 -10.09 -3.73 -5.94 8.57 -0.23 -13.84 -4.05 -4.43 10.92
Endosome -5.46 6.66 -2.67 -0.49 -5.45 -7.96 12.18 -9.95 -3.07 -18.03 -10.70 18.02 -10.35 -3.09 -21.39
Golgi -5.00 2.31 -2.38 -2.01 -2.47 -2.10 6.28 -4.47 -2.21 -8.23 -3.32 6.43 -5.06 -3.07 -7.83
Lipid Particles 1.60 4.58 5.52 4.48 4.23 4.31 7.77 5.53 1.74 -1.76 4.89 9.16 5.89 1.72 -2.57
Mitochondria -6.70 -0.94 -1.23 4.98 -1.09 -1.49 6.69 0.75 2.10 -5.21 -2.99 6.51 0.53 3.34 -5.13
Mitotic Spindle -3.82 -5.67 -2.29 -2.01 -1.54 -2.71 -2.18 -5.61 -2.59 -2.75 -2.92 -4.59 -6.10 -4.16 -2.02
None -0.85 -9.52 3.93 1.87 13.10 0.12 -6.13 -1.89 -1.95 4.36 -0.48 -9.72 -1.81 -1.11 5.92
Nuclear Periphery 0.27 -14.82 -1.43 -1.45 0.17 1.55 -12.72 -6.24 -7.23 8.47 1.39 -18.52 -5.42 -6.09 9.33
Nuclear Periphery Foci 2.36 -6.06 -1.37 -1.43 -0.86 -0.14 -5.53 -3.16 -3.16 1.50 0.06 -8.46 -3.41 -3.44 -0.10
Nucleolus -0.74 -4.08 -1.12 -0.99 0.58 -2.36 -2.30 -5.44 -5.09 -1.34 -3.02 -4.89 -5.50 -5.15 -2.49
Nucleus 5.95 -37.40 7.70 5.36 59.42 10.06 -30.30 0.99 -6.52 29.93 13.91 -58.54 1.95 -7.86 55.84
Peroxisomes -2.12 1.61 0.15 1.94 -1.13 -2.87 0.99 -2.30 -0.60 -2.22 -2.25 3.76 -2.35 -1.23 -4.30
Vacuole -10.97 8.70 -8.78 -1.90 -17.61 -10.69 9.82 -20.59 -9.77 -25.95 -19.70 22.20 -22.04 -5.53 -43.67
Vacuole Periphery -5.09 1.81 -5.81 -4.93 -6.20 -8.58 13.69 -14.88 -12.93 -16.17 -13.72 9.48 -15.70 -11.35 -17.07
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YLR297W

YLR297W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr297w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available