Standard name
Human Ortholog
Description Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 – 0 0 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.07 0.05 0.09 0.07 – 0.05 0.07 0.07 0 0.07 0.11 0.18 0.46 0.29 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.14 0 0 0 0.31 0.06 – 0.24 0.13 0.17 0.19 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.88 0.9 0.98 0.91 0.92 0.82 0.86 – 0.84 0.83 0.79 0.87 0.9 0.88 0.79 0.5 0.64 0.76 0.76 0.87 0.83 0.76 0.65 0.59
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0.1 0 0.11 0.11 0.14 0.13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.07 0.05 0.05 0 0 0.06 0.07 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 1 1 – 3 3 3 2 0 0 0 0 0 0 1 0 0 2 3 7
Bud Neck 0 0 0 0 2 0 1 – 0 2 3 3 0 0 0 0 0 0 0 0 0 0 3 1
Bud Site 0 0 0 1 1 10 2 – 11 5 16 13 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7 2 2 13 13 18 26 – 14 22 14 11 10 29 57 57 22 14 0 0 0 2 2 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 6 3 3 0 0 0 0 2 1
Endosome 0 0 1 1 2 0 3 – 1 2 1 1 0 2 1 3 1 3 2 1 0 0 7 8
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 0 0 1 4 3
Mitochondria 10 22 2 4 2 65 23 – 66 39 35 48 4 5 2 4 3 5 0 2 0 1 4 6
Nucleus 133 138 174 176 259 172 309 – 231 258 167 214 131 233 248 62 48 73 121 136 153 146 238 181
Nuclear Periphery 0 0 0 1 0 0 0 – 2 0 1 1 0 2 1 0 2 0 0 0 0 0 1 2
Nucleolus 6 3 0 0 2 5 1 – 11 4 15 6 4 2 5 1 3 0 16 6 21 21 51 41
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 1 11 6 8 1 20 – 4 8 5 9 1 2 1 1 0 1 1 0 2 4 17 10
Vac/Vac Membrane 3 4 1 2 5 1 6 – 1 6 4 1 1 2 5 7 5 5 7 6 5 11 25 35
Unique Cell Count 151 153 178 193 281 211 361 276 310 212 247 145 265 313 124 75 96 160 157 186 193 368 306
Labelled Cell Count 160 171 191 204 294 273 392 344 349 264 309 152 278 320 141 87 105 160 157 186 193 368 306


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.0 5.9 6.3 6.9 4.4 5.9 – 5.4 5.4 5.6 5.6 5.9 6.0 6.2 8.1 7.9 7.5 5.3 5.9 5.7
Std Deviation (1e-4) 0.9 0.7 0.9 1.6 1.6 1.4 1.8 – 1.6 1.2 1.6 1.5 1.0 1.6 1.7 2.3 2.1 1.8 1.3 1.7 1.5
Intensity Change (Log2) – – – 0.11 0.23 -0.41 0.01 – -0.12 -0.11 -0.07 -0.07 0.01 0.03 0.09 0.46 0.43 0.35 -0.14 0.0 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 3.7 3.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 2.1 3.3 3.0 – 2.2 2.9 2.7 2.0 2.7 4.0 5.6 9.7 7.0 4.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.7 – 6.6 4.4 5.2 5.8 0 0 0 0 0 0
Nucleus -2.7 -2.5 -5.1 -4.4 – -4.7 -4.8 -5.6 -4.0 -2.9 -3.7 -5.7 -9.9 -7.5 -5.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 3.6 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.4 -1.7 0 -0.3 – -2.8 -2.0 -1.9 -1.2 -2.6 -3.3 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1472 0.0866 -0.0851 -0.3649 -0.4891 -0.1666 1.223 1.4266 1.3631 1.381 1.0498 1.1248 3.0725 3.2768 2.9585 2.9417 2.8469 2.9633
Actin 0.0088 0 0.004 0 0.0032 0.0009 0.0109 0.0003 0.0011 0.0012 0.0013 0 0.0153 0.0001 0 0 0.0015 0.0021
Bud 0.0002 0 0.0003 0.0003 0.0001 0 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0 0 0.0001 0
Bud Neck 0.0015 0.0001 0.0003 0.0022 0.0007 0.0008 0.0014 0.0002 0.0003 0.0006 0.0014 0.0019 0.01 0.0002 0.0001 0 0.0003 0.0013
Bud Periphery 0.0007 0 0.0006 0.0004 0.0002 0.0001 0.0004 0.0001 0.0007 0.0002 0.0005 0.0001 0.0008 0.0001 0 0 0.0001 0.0001
Bud Site 0.0008 0.0001 0.0019 0.0023 0.0006 0.0001 0.0015 0.0003 0.0009 0.0007 0.0022 0.0002 0.001 0.0004 0.0001 0 0.0001 0.0001
Cell Periphery 0.0002 0 0.0001 0.0003 0 0 0.0001 0 0.0001 0.0002 0.0003 0 0.0002 0 0 0 0 0
Cytoplasm 0.0007 0.0015 0.0006 0.006 0.0033 0.0002 0.01 0.0006 0.0007 0.0003 0.0002 0.0002 0.0049 0.0068 0.0001 0.0001 0.0056 0.0004
Cytoplasmic Foci 0.0162 0 0.0019 0.0012 0.016 0.0005 0.0217 0.0003 0.0014 0.0236 0.0041 0.0012 0.0039 0.0002 0.0004 0 0.0004 0.0003
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0001 0.0003 0.0003 0.001 0.0004 0.0037 0.0001 0.0009 0.0008 0.0003 0.0001 0.0017 0.0001 0 0 0.0009 0.0002
Endosome 0.0112 0.0002 0.0023 0.0029 0.0228 0.005 0.0127 0.0001 0.0175 0.037 0.0074 0.0061 0.0036 0.0001 0.0005 0 0.0029 0.0013
Golgi 0.0031 0 0.0006 0.0001 0.0058 0.0013 0.006 0.0001 0.0048 0.0209 0.005 0.0005 0.0011 0 0.0003 0 0.0002 0.0004
Lipid Particles 0.0223 0 0.0068 0.0001 0.0391 0.0032 0.0063 0.0001 0.007 0.0656 0.0397 0.0003 0.003 0 0.0068 0 0.0002 0.0009
Mitochondria 0.0084 0.0001 0.0092 0.0007 0.0111 0.007 0.003 0.0089 0.0073 0.013 0.0172 0.0004 0.004 0.0001 0.0002 0 0.0011 0.0003
None 0.002 0.0008 0.0004 0.0008 0.0176 0.0002 0.0047 0.0001 0.0002 0.0002 0.0002 0 0.0161 0.0122 0.0001 0 0.0177 0.0023
Nuclear Periphery 0.0078 0.0257 0.0096 0.0008 0.0145 0.0078 0.02 0.0022 0.0052 0.002 0.002 0.0038 0.0112 0.0012 0.0005 0.0022 0.0095 0.0014
Nucleolus 0.0182 0.0148 0.0175 0.0173 0.0496 0.0468 0.0207 0.0167 0.0147 0.0052 0.0564 0.0647 0.0161 0.0201 0.0144 0.0056 0.0155 0.0367
Nucleus 0.874 0.9511 0.9356 0.9462 0.7827 0.9159 0.8462 0.9557 0.929 0.8033 0.8488 0.912 0.8857 0.9537 0.9724 0.9915 0.8978 0.9453
Peroxisomes 0.0074 0 0.0017 0.0001 0.017 0.003 0.0144 0.0002 0.0006 0.0168 0.0075 0.0001 0.0118 0 0.0012 0 0.0001 0.0003
Punctate Nuclear 0.0109 0.0051 0.0038 0.0073 0.0125 0.005 0.0139 0.0139 0.0017 0.001 0.0021 0.0069 0.0076 0.0043 0.0029 0.0005 0.0447 0.0063
Vacuole 0.0032 0.0001 0.0016 0.0097 0.0011 0.0008 0.0012 0.0001 0.0039 0.0057 0.0021 0.0008 0.001 0.0001 0 0 0.0006 0.0001
Vacuole Periphery 0.0015 0.0001 0.001 0.001 0.0009 0.0008 0.0007 0 0.0015 0.0016 0.0012 0.0005 0.0006 0 0 0 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.0975 9.9205 5.2581 14.4566 9.2496 3.1689 15.7623 16.9249 15.1451 15.3399
Translational Efficiency 2.7968 2.8812 4.3729 1.4389 2.6937 8.2445 1.4602 1.7216 1.7475 1.7907

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2216 1238 759 1573 941 2511 1655 1301 3157 3749 2414 2874

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 719.95 754.98 955.27 918.04 681.47 802.95 900.04 963.97 708.48 787.11 917.41 938.83
Standard Deviation 90.43 117.86 124.30 140.43 113.67 103.04 123.55 135.28 99.51 110.49 126.41 140.00
Intensity Change Log 2 — 0.068542 0.408012 0.350660 — 0.236660 0.401339 0.500338 — 0.152741 0.404771 0.425385

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.000798 0.001062 0.001016 0.000165 0.000263 0.000837 0.000840 0.000173 0.000440 0.000908 0.000936
Bud Neck 0.003521 0.009250 0.011810 0.023745 0.001697 0.012707 0.014427 0.025468 0.002977 0.011565 0.013604 0.024525
Bud Site 0.000704 0.001780 0.002933 0.006323 0.000392 0.002890 0.001702 0.003544 0.000611 0.002523 0.002089 0.005065
Cell Periphery 0.000111 0.000204 0.000076 0.000327 0.000099 0.000114 0.000056 0.000085 0.000107 0.000144 0.000062 0.000218
Cytoplasm 0.010737 0.015812 0.003472 0.010387 0.017965 0.008266 0.000977 0.004509 0.012891 0.010758 0.001761 0.007726
Cytoplasmic Foci 0.003349 0.003090 0.000650 0.001107 0.006553 0.001631 0.000031 0.001401 0.004304 0.002113 0.000225 0.001240
Eisosomes 0.000020 0.000030 0.000039 0.000030 0.000024 0.000023 0.000028 0.000026 0.000021 0.000025 0.000032 0.000028
Endoplasmic Reticulum 0.001924 0.005525 0.005489 0.005474 0.001550 0.002835 0.004723 0.005771 0.001813 0.003723 0.004964 0.005609
Endosome 0.000334 0.001090 0.000350 0.002579 0.000644 0.000970 0.000163 0.001025 0.000427 0.001009 0.000221 0.001875
Golgi 0.000336 0.000759 0.000113 0.000688 0.000216 0.000324 0.000089 0.000511 0.000300 0.000468 0.000097 0.000608
Lipid Particles 0.001299 0.000981 0.000319 0.000290 0.001096 0.000752 0.000102 0.000100 0.001238 0.000827 0.000171 0.000204
Mitochondria 0.002693 0.003174 0.001178 0.005635 0.001371 0.004445 0.001509 0.001901 0.002299 0.004026 0.001405 0.003945
Mitotic Spindle 0.000466 0.003496 0.002209 0.035824 0.003552 0.008345 0.001150 0.008228 0.001385 0.006744 0.001483 0.023332
None 0.009221 0.022473 0.003005 0.009944 0.027543 0.006270 0.003142 0.003917 0.014682 0.011620 0.003099 0.007215
Nuclear Periphery 0.001250 0.000914 0.000446 0.002597 0.000524 0.002309 0.000356 0.001252 0.001034 0.001848 0.000384 0.001989
Nuclear Periphery Foci 0.000500 0.001261 0.000280 0.001142 0.000739 0.000709 0.000203 0.000274 0.000571 0.000891 0.000227 0.000749
Nucleolus 0.027028 0.034517 0.021656 0.010119 0.022210 0.030905 0.020961 0.010797 0.025592 0.032098 0.021180 0.010426
Nucleus 0.934206* 0.891748* 0.941889* 0.867011* 0.911992* 0.912602* 0.948132* 0.926756* 0.927585* 0.905716* 0.946169* 0.894056*
Peroxisomes 0.001526 0.001331 0.000420 0.000340 0.000691 0.001596 0.000292 0.000167 0.001277 0.001509 0.000332 0.000262
Vacuole 0.000357 0.001195 0.002416 0.011562 0.000738 0.001445 0.001005 0.002857 0.000470 0.001363 0.001449 0.007622
Vacuole Periphery 0.000243 0.000572 0.000187 0.003859 0.000240 0.000599 0.000115 0.000570 0.000242 0.000590 0.000137 0.002370

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.72 -14.82 -18.60 -3.21 2.52 -3.26 -15.45 -17.97 -16.14 -1.60 -6.92 -20.24 -24.66 -14.28 -0.50
Bud Neck -7.39 -11.64 -15.87 -10.05 -6.57 -12.46 -21.12 -20.07 -10.24 -7.20 -13.34 -22.83 -24.53 -13.45 -9.11
Bud Site -3.81 -2.20 -7.39 -5.32 -1.15 -4.01 -2.56 -4.69 -0.76 -2.04 -4.32 -3.03 -8.27 -3.02 -2.97
Cell Periphery -3.62 2.07 -1.22 0.09 -1.72 -0.42 1.93 0.61 1.63 -4.47 -2.00 3.45 -0.98 -0.12 -2.20
Cytoplasm -2.29 6.75 0.76 2.88 -6.27 4.30 7.78 6.07 3.76 -5.38 2.02 11.47 5.06 3.32 -9.28
Cytoplasmic Foci 0.41 4.54 4.70 3.50 0.01 3.85 5.05 4.22 1.28 -1.38 3.65 7.26 5.98 3.47 -1.63
Eisosomes -5.04 -10.01 -10.75 -2.56 3.81 0.89 -2.01 -1.45 -2.40 0.83 -1.99 -9.98 -9.24 -4.03 1.89
Endoplasmic Reticulum -11.52 -13.92 -15.46 -1.46 0.78 -5.78 -10.23 -16.98 -12.39 -3.86 -11.43 -15.08 -22.87 -11.01 -2.89
Endosome -2.81 -0.80 -4.41 -0.97 -4.26 -0.86 2.62 -0.20 0.47 -2.00 -3.24 2.88 -3.39 -0.60 -4.69
Golgi -1.39 1.93 -0.43 1.24 -3.37 -0.60 1.78 -0.55 -0.20 -1.25 -1.10 2.58 -0.54 0.54 -2.60
Lipid Particles 1.20 4.89 5.69 3.51 1.98 1.61 4.39 4.29 6.37 -1.28 2.54 7.21 7.19 6.61 -0.30
Mitochondria -0.54 3.09 -1.60 -0.90 -4.00 -4.63 -0.21 -2.09 3.96 -2.36 -3.22 2.17 -1.84 1.42 -4.17
Mitotic Spindle -3.01 -2.24 -8.30 -6.55 -6.28 -2.29 0.92 -2.69 -0.90 -4.10 -5.02 -1.43 -8.83 -5.52 -8.13
None -4.70 6.63 1.52 5.42 -4.49 6.34 7.32 7.13 3.96 -1.02 2.45 9.67 7.02 5.45 -4.37
Nuclear Periphery 1.73 4.74 -2.02 -2.63 -3.42 -5.43 0.31 -5.45 1.14 -6.58 -3.61 4.83 -3.50 -0.73 -5.47
Nuclear Periphery Foci -2.55 1.16 -1.37 1.41 -2.26 0.28 2.61 2.14 2.16 -1.33 -1.50 3.22 0.15 1.63 -3.12
Nucleolus -3.21 1.14 11.96 12.28 6.47 -3.73 0.46 5.33 11.29 6.03 -4.06 2.31 12.73 16.96 9.33
Nucleus 7.13 0.19 8.03 0.80 7.24 -0.70 -5.25 -1.41 -1.07 5.66 4.93 -4.23 5.77 1.22 10.28
Peroxisomes 0.44 2.66 3.15 3.93 5.23 -1.50 2.57 2.98 4.29 3.63 -0.22 3.42 3.81 5.46 4.84
Vacuole -4.12 -4.96 -7.60 -6.96 -5.19 -2.17 -1.35 -4.18 -3.28 -3.54 -4.55 -5.07 -8.73 -7.57 -6.87
Vacuole Periphery -2.46 0.56 -3.51 -2.79 -3.63 -2.49 1.10 -1.02 0.79 -1.91 -3.68 1.60 -3.66 -2.40 -4.09
Standard name
Human Ortholog
Description Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Yhc1

Yhc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available