Standard name | |
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Human Ortholog | |
Description | Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 4 | – | – | – | 4 | 5 | 5 | 0 | 2 | 2 |
Bud | 1 | 13 | 0 | 0 | 2 | 3 | 2 | 1 | – | 2 | 2 | 1 | 3 | 2 | 1 | – | – | – | 2 | 4 | 2 | 0 | 4 | 10 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 5 | 4 | 2 | 4 | 7 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 37 | 33 | 28 | 9 | 12 | 2 | 13 | 19 | – | 13 | 7 | 11 | 27 | 29 | 18 | – | – | – | 2 | 2 | 4 | 1 | 3 | 3 |
Cytoplasm | 493 | 472 | 200 | 135 | 212 | 134 | 181 | 214 | – | 123 | 43 | 69 | 218 | 267 | 369 | – | – | – | 444 | 439 | 155 | 345 | 334 | 383 |
Endoplasmic Reticulum | 4 | 6 | 1 | 0 | 1 | 2 | 3 | 5 | – | 0 | 2 | 1 | 1 | 3 | 2 | – | – | – | 9 | 8 | 8 | 15 | 12 | 21 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | 2 | 1 | 0 | 2 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 2 | 2 | 0 | 0 | 1 | 2 |
Mitochondria | 1 | 0 | 1 | 3 | 5 | 30 | 46 | 41 | – | 68 | 33 | 46 | 0 | 0 | 0 | – | – | – | 4 | 4 | 5 | 6 | 6 | 1 |
Nucleus | 3 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 1 | – | – | – | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | – | 1 | 0 | 3 | 0 | 0 | 2 | – | – | – | 2 | 10 | 4 | 3 | 3 | 5 |
Unique Cell Count | 496 | 480 | 202 | 136 | 217 | 142 | 206 | 242 | 161 | 70 | 100 | 219 | 270 | 373 | 486 | 492 | 194 | 390 | 383 | 451 | ||||
Labelled Cell Count | 542 | 532 | 232 | 147 | 238 | 171 | 247 | 282 | 208 | 88 | 134 | 250 | 301 | 397 | 486 | 492 | 194 | 390 | 383 | 451 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.7 | 6.4 | 6.6 | 6.1 | 6.3 | 5.1 | 5.1 | 5.6 | – | 4.7 | 5.1 | 4.8 | 6.8 | 6.6 | 6.9 | – | – | – | 6.6 | 7.4 | 7.7 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.7 | 1.9 | 2.3 | 1.8 | 2.1 | 1.8 | – | 1.7 | 1.0 | 1.4 | 0.9 | 0.7 | 0.9 | – | – | – | 1.2 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.07 | -0.36 | -0.36 | -0.22 | – | -0.48 | -0.36 | -0.45 | 0.04 | 0.01 | 0.07 | – | – | – | 0.01 | 0.17 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -2.1 | -2.9 | -4.0 | -2.5 | -2.0 | – | 0 | -0.8 | 0 | -0.5 | -1.0 | -3.8 | – | – | – |
Cytoplasm | 0.2 | -1.0 | -2.5 | -4.5 | -4.4 | – | -6.8 | -8.8 | -7.9 | 0.7 | -0.1 | -0.1 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 6.6 | 6.9 | 5.9 | – | 0 | 10.2 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5124 | 2.204 | 1.7967 | 1.5734 | 1.9269 | 1.839 | 3.8788 | 4.393 | 4.4738 | 4.5358 | 4.6115 | 4.5834 | 2.8859 | 3.4441 | 3.9236 | 3.5745 | 3.3202 | 3.7194 |
Actin | 0.0417 | 0.0137 | 0.0129 | 0.0348 | 0.0383 | 0.005 | 0.0237 | 0.0023 | 0.0206 | 0.0134 | 0.0063 | 0.0127 | 0.0002 | 0.0009 | 0.0148 | 0.0499 | 0.0004 | 0.002 |
Bud | 0.0015 | 0.0042 | 0.0008 | 0.0009 | 0.0051 | 0.0001 | 0.0008 | 0.0025 | 0.0072 | 0.0015 | 0.003 | 0.0026 | 0.0001 | 0 | 0.0007 | 0.0296 | 0 | 0.0003 |
Bud Neck | 0.0014 | 0.0017 | 0.0013 | 0.0006 | 0.0024 | 0.0009 | 0.001 | 0.0006 | 0.0026 | 0.0102 | 0.0132 | 0.0018 | 0.0001 | 0.0002 | 0.0054 | 0.004 | 0.0003 | 0.0006 |
Bud Periphery | 0.0019 | 0.0068 | 0.0009 | 0.002 | 0.0136 | 0.0002 | 0.0023 | 0.003 | 0.007 | 0.0027 | 0.0093 | 0.0114 | 0.0002 | 0 | 0.0012 | 0.198 | 0.0001 | 0.0027 |
Bud Site | 0.0063 | 0.0318 | 0.0171 | 0.0045 | 0.0104 | 0.0003 | 0.0089 | 0.0599 | 0.023 | 0.0219 | 0.0143 | 0.0021 | 0.0011 | 0.0006 | 0.0153 | 0.0059 | 0 | 0.0001 |
Cell Periphery | 0.0023 | 0.0016 | 0.0031 | 0.0074 | 0.001 | 0.0011 | 0.008 | 0.0045 | 0.0054 | 0.0026 | 0.0035 | 0.0051 | 0.0065 | 0.0012 | 0.0022 | 0.0037 | 0.0004 | 0.0014 |
Cytoplasm | 0.4751 | 0.7572 | 0.7214 | 0.7551 | 0.5446 | 0.7639 | 0.508 | 0.6797 | 0.5647 | 0.4838 | 0.3807 | 0.623 | 0.6478 | 0.937 | 0.6268 | 0.4665 | 0.625 | 0.7455 |
Cytoplasmic Foci | 0.0274 | 0.009 | 0.017 | 0.0161 | 0.0278 | 0.012 | 0.0149 | 0.0044 | 0.0214 | 0.0213 | 0.0169 | 0.005 | 0.0193 | 0.0022 | 0.0291 | 0.0782 | 0.0016 | 0.0022 |
Eisosomes | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0014 | 0.0006 | 0.0006 | 0.0008 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0101 | 0.0047 | 0.007 | 0.0124 | 0.0102 | 0.0072 | 0.0066 | 0.0029 | 0.005 | 0.0067 | 0.0037 | 0.0035 | 0.0068 | 0.0132 | 0.0081 | 0.0018 | 0.0007 | 0.0033 |
Endosome | 0.018 | 0.0057 | 0.0106 | 0.0144 | 0.0431 | 0.0061 | 0.0111 | 0.0009 | 0.0084 | 0.0122 | 0.0223 | 0.0027 | 0.0194 | 0.0008 | 0.0096 | 0.008 | 0.0001 | 0.0006 |
Golgi | 0.0075 | 0.0018 | 0.0036 | 0.0052 | 0.026 | 0.002 | 0.0032 | 0.0003 | 0.0034 | 0.0051 | 0.0044 | 0.0012 | 0.0019 | 0.0004 | 0.0117 | 0.0206 | 0.0001 | 0.0002 |
Lipid Particles | 0.0071 | 0.0014 | 0.0014 | 0.0022 | 0.0226 | 0.0025 | 0.0079 | 0.0004 | 0.0038 | 0.0096 | 0.0035 | 0.0009 | 0.0175 | 0.0004 | 0.0074 | 0.0155 | 0.0001 | 0.0002 |
Mitochondria | 0.0022 | 0.0009 | 0.0012 | 0.0023 | 0.021 | 0.0004 | 0.0017 | 0.0003 | 0.0008 | 0.0028 | 0.0408 | 0.0016 | 0.0012 | 0 | 0.0038 | 0.0059 | 0.0001 | 0.0001 |
None | 0.3504 | 0.153 | 0.1922 | 0.1291 | 0.1979 | 0.193 | 0.3813 | 0.2338 | 0.3073 | 0.3338 | 0.4524 | 0.3211 | 0.1962 | 0.0413 | 0.2518 | 0.0287 | 0.3705 | 0.2398 |
Nuclear Periphery | 0.0062 | 0.0005 | 0.0009 | 0.0028 | 0.003 | 0.0009 | 0.0039 | 0.0004 | 0.0013 | 0.0045 | 0.0014 | 0.0008 | 0.0463 | 0.0005 | 0.0012 | 0.0002 | 0 | 0.0001 |
Nucleolus | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0015 | 0.0001 | 0.0008 | 0.0004 | 0.0004 | 0.0026 | 0.002 | 0.0002 | 0.002 | 0 | 0.001 | 0.0001 | 0 | 0 |
Nucleus | 0.006 | 0.0008 | 0.0008 | 0.0019 | 0.0031 | 0.0004 | 0.0013 | 0.0009 | 0.0012 | 0.0428 | 0.0029 | 0.0007 | 0.0113 | 0.0004 | 0.0015 | 0.0004 | 0.0001 | 0.0002 |
Peroxisomes | 0.0066 | 0.0024 | 0.0039 | 0.0025 | 0.0163 | 0.0013 | 0.0031 | 0.0003 | 0.0083 | 0.0106 | 0.0028 | 0.0004 | 0.0014 | 0 | 0.0036 | 0.081 | 0 | 0 |
Punctate Nuclear | 0.0225 | 0.0007 | 0.0007 | 0.0033 | 0.0054 | 0.0011 | 0.005 | 0.0006 | 0.004 | 0.0061 | 0.0023 | 0.0006 | 0.0065 | 0.0001 | 0.0014 | 0.0009 | 0 | 0.0001 |
Vacuole | 0.0036 | 0.0014 | 0.0023 | 0.0016 | 0.0043 | 0.0013 | 0.0043 | 0.0015 | 0.0032 | 0.0046 | 0.0117 | 0.0021 | 0.0059 | 0.0005 | 0.0028 | 0.0006 | 0.0003 | 0.0006 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0018 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0006 | 0.0021 | 0.0002 | 0.0082 | 0 | 0.0004 | 0.0002 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.9008 | 20.2652 | 22.8633 | 32.9606 | 24.8933 | 17.8019 | 31.676 | 34.3619 | 30.4591 | 34.7056 |
Translational Efficiency | 0.4657 | 0.5757 | 0.5046 | 0.3471 | 0.4115 | 0.6164 | 0.3763 | 0.3313 | 0.4307 | 0.3798 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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