Standard name
Human Ortholog
Description Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0.13 0.05 0.06 0 – 0.09 0.11 0.08 0.08 0.09 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.05 0.06 0.08 0.07 – 0.08 0 0.07 0.06 0.07 0.14 0.16 0.25 0.12 0.24 0.12 0 0 0 0.05 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.23 0.49 0 0.11 0.2 0.16 – 0.11 0.07 0.07 0.11 0.07 0.4 0.49 0.44 0.44 0.22 0.4 0.1 0.08 0.07 0.1 0.11 0.12
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.05 0.48 0.43 0.12 0.33 – 0.23 0.36 0.29 0.26 0.39 0.06 0 0 0.07 0.07 0 0 0 0 0 0 0
Nucleus 0.05 0 0.09 0 0 0.05 – 0.06 0.05 0.07 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.66 0.5 0.3 0.4 0.32 0.44 – 0.43 0.44 0.39 0.46 0.4 0.45 0.36 0.32 0.43 0.4 0.41 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.35 0.45 0.53 0.53 0.62 0.48 – 0.55 0.54 0.51 0.54 0.48 0.39 0.32 0.28 0.6 0.48 0.54 0.75 0.76 0.75 0.65 0.6 0.55
Vac/Vac Membrane 0 0.06 0 0 0 0 – 0 0 0 0 0 0.08 0.09 0.12 0.07 0.12 0.11 0 0 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 – 1 0 0 0 2 0 0 0 0 0 2 0 0 0 0 0 3
Bud 0 0 3 1 1 4 – 6 9 6 3 9 0 1 0 3 4 2 8 2 1 2 4 8
Bud Neck 0 0 2 0 0 0 – 2 8 5 1 2 0 1 0 0 3 2 4 1 1 0 1 0
Bud Site 10 1 10 5 6 6 – 20 23 16 8 15 1 3 0 3 7 4 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 9 4 6 8 10 – 19 8 14 6 13 12 28 33 40 79 38 2 1 2 4 6 10
Endoplasmic Reticulum 3 0 2 1 0 0 – 0 0 0 0 0 1 1 2 6 11 12 0 0 0 0 0 0
Endosome 79 54 2 11 20 21 – 25 15 14 11 12 35 84 59 146 71 127 38 9 6 9 16 21
Golgi 0 1 0 0 1 0 – 0 0 0 0 0 1 1 1 0 0 0 4 3 0 2 2 6
Mitochondria 61 6 38 43 12 44 – 54 74 60 27 68 5 6 2 22 22 9 3 1 2 1 4 4
Nucleus 17 1 7 2 4 7 – 14 10 14 10 9 2 2 3 9 12 13 8 2 2 3 5 8
Nuclear Periphery 1 0 0 2 0 2 – 1 1 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1
Nucleolus 228 55 24 40 32 60 – 101 90 81 47 70 40 62 42 143 131 131 15 4 2 1 4 3
Peroxisomes 1 2 0 3 5 3 – 3 9 2 2 6 0 3 1 8 0 1 1 0 0 1 0 0
SpindlePole 120 50 42 52 63 65 – 129 112 105 55 85 34 54 37 198 157 172 286 96 79 57 91 97
Vac/Vac Membrane 9 7 1 3 3 3 – 5 5 1 0 2 7 15 16 23 41 35 5 1 3 2 9 6
Unique Cell Count 347 110 79 99 101 135 235 206 206 102 176 88 171 133 330 330 318 383 127 105 89 152 179
Labelled Cell Count 541 186 135 169 155 225 380 364 318 171 293 138 261 196 601 538 548 383 127 105 89 152 179


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.8 3.9 4.7 5.6 4.4 – 4.5 4.1 4.3 5.3 4.1 5.9 6.2 6.5 7.6 6.6 7.3 5.7 6.1 6.3
Std Deviation (1e-4) 1.1 1.3 0.7 1.2 1.9 1.4 – 1.2 1.7 1.4 8.5 1.4 1.2 1.1 1.7 1.3 1.3 1.2 1.1 1.3 1.8
Intensity Change (Log2) – – – 0.27 0.51 0.18 – 0.2 0.07 0.13 0.43 0.05 0.59 0.66 0.74 0.97 0.76 0.91 0.54 0.63 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud Site – -1.6 0 – 0 – 0 -1.1 0 -3.0 0 0 0 0 0
Cell Periphery – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Endosome – 3.5 0 – 0 – 0 2.1 0 5.8 7.2 6.5 6.9 4.0 6.4
Golgi – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Mitochondria – -5.4 0 – 0 – 0 -3.0 0 -6.3 -8.6 -8.4 -9.3 -9.3 -11.1
Nuclear Periphery – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – 0.2 0 – 0 – 0 2.1 0 2.0 0.9 0.2 2.1 1.5 1.8
Peroxisomes – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
SpindlePole – 1.2 0 – 0 – 0 0.1 0 -1.9 -3.3 -3.7 1.1 -0.9 0.1
Vac – – – – – – – – – – – – – – –
Cortical Patches – 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.8 0.7 – 0.9 – 0 0 0.7 1.9 2.5 3.7 1.8 3.7 1.8
Nucleus – 0 -1.0 – -0.9 – -0.6 0.2 0 0 0 0 0 0 0
Vacuole – 0 0 – 0 – 0 0 0 0 2.3 2.8 0 2.9 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.739 2.1909 1.6768 1.5893 1.005 1.808 1.3994 1.669 1.6479 1.4039 1.2542 1.5151 2.9265 2.795 2.8287 2.8093 2.8528 2.6035
Actin 0.0162 0.0001 0.0003 0.0014 0.0002 0.0001 0.0132 0.002 0.0075 0.0083 0.0753 0.0098 0.0212 0.0013 0.0038 0.007 0.0043 0.0061
Bud 0.0002 0.0001 0 0.0007 0.0001 0.0001 0.0003 0.0001 0.0001 0.0026 0.0006 0 0.0003 0.0002 0.0001 0.0003 0.0004 0.0002
Bud Neck 0.0007 0.0011 0.0009 0.004 0.0016 0.0036 0.0018 0.0011 0.0008 0.0009 0.0007 0.0007 0.0016 0.0019 0.0007 0.0021 0.0021 0.0005
Bud Periphery 0.0003 0 0 0.0006 0.0001 0.0001 0.0004 0.0001 0.0001 0.0048 0.0009 0 0.0005 0.0003 0.0001 0.0009 0.001 0.0011
Bud Site 0.0009 0.0024 0.0002 0.0021 0.0009 0.0004 0.0104 0.0054 0.0007 0.0278 0.0019 0.0001 0.0017 0.0028 0.0007 0.0025 0.0035 0.0032
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0021 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0022 0.0214 0.0063 0.001 0.0011 0.0057 0.0041 0.0065 0.0008 0.0013 0.0004 0.004 0.0017 0.0061 0.0025 0.0013 0.0004 0.0075
Cytoplasmic Foci 0.0741 0.0695 0.0367 0.0468 0.0824 0.0274 0.054 0.0468 0.0241 0.1321 0.0874 0.0088 0.0496 0.032 0.0143 0.0292 0.063 0.0217
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0011 0.0003 0.0002 0.0001 0.0011 0.0005 0.003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0026 0.0001 0.003 0.0005 0.0003 0.0014
Endosome 0.0175 0.0031 0.0087 0.0112 0.0147 0.0021 0.0386 0.0046 0.006 0.0029 0.0155 0.0006 0.0308 0.0019 0.0046 0.0083 0.0132 0.0084
Golgi 0.0053 0.001 0.0012 0.0123 0.0053 0.0007 0.0079 0.0009 0.0063 0.0028 0.0162 0.0005 0.016 0.0011 0.004 0.009 0.0103 0.0017
Lipid Particles 0.0194 0.01 0.0071 0.0168 0.0363 0.0061 0.021 0.0017 0.0125 0.0121 0.0726 0.0024 0.0276 0.0027 0.0034 0.0273 0.1155 0.0026
Mitochondria 0.0073 0.0001 0 0.0016 0.0571 0 0.003 0 0.01 0.0032 0.008 0.0001 0.0055 0.0002 0.0012 0.0071 0.0159 0.0021
None 0.0131 0.0011 0.0002 0.0005 0.0003 0.0044 0.0044 0.0007 0.0006 0.0003 0.0005 0.0015 0.0025 0.0021 0.0018 0.0007 0.0006 0.0047
Nuclear Periphery 0.0136 0.0017 0.0012 0.0009 0.0022 0.0019 0.0088 0.0007 0.0005 0.0008 0.0032 0.0005 0.0128 0.0021 0.0081 0.0055 0.0115 0.0081
Nucleolus 0.007 0.0152 0.0048 0.0068 0.048 0.0089 0.0094 0.0106 0.008 0.0007 0.0021 0.003 0.012 0.0457 0.02 0.0025 0.011 0.01
Nucleus 0.0143 0.0557 0.0075 0.0153 0.01 0.0214 0.0097 0.0455 0.02 0.0006 0.0004 0.0059 0.0047 0.0688 0.0196 0.0418 0.0019 0.0139
Peroxisomes 0.0159 0.0032 0.0017 0.0151 0.0064 0.0016 0.0173 0.0016 0.0044 0.0144 0.0338 0.0005 0.0085 0.0025 0.0062 0.0114 0.0567 0.0018
Punctate Nuclear 0.7855 0.8134 0.9226 0.8625 0.7305 0.9147 0.787 0.8712 0.8965 0.7807 0.6793 0.9614 0.796 0.8276 0.9051 0.8413 0.6866 0.9033
Vacuole 0.0023 0.0004 0.0003 0.0003 0.0009 0.0002 0.0035 0.0003 0.0005 0.001 0.0003 0 0.0019 0.0004 0.0004 0.0006 0.001 0.0005
Vacuole Periphery 0.0031 0 0.0001 0.0001 0.0007 0 0.002 0 0.0005 0.0001 0.0003 0 0.0021 0.0001 0.0003 0.0006 0.0006 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –
Standard name
Human Ortholog
Description Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
Localization
Cell Percentages spindle pole (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nkp2

Nkp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nkp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available