Standard name
Human Ortholog
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.36 0.24 0.23 0.23 0 0.09 0.09 0.06 – 0.06 0.05 0.31 0.15 0.08 0.33 0.29 0.38 0.36 0.24 0.2 0.21 0.27 0.34
Bud 0.26 0.22 0.19 0.25 0.19 0.17 0.16 0.12 0.17 – 0.14 0.16 0.19 0.13 0.14 0.12 0.12 0.13 0.23 0.23 0.21 0.33 0.32 0.29
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.2 0.23 0.22 0.21 0.14 0.11
Bud Site 0.12 0.18 0.1 0.13 0.14 0.12 0.07 0 0.05 – 0 0 0.17 0 0 0.05 0.05 0.05 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0.06 0.11 0.08 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.26 0.29 0.46 0.41 0.4 0.39 0.46 0.51 0.41 – 0.41 0.47 0.34 0.63 0.61 0.28 0.24 0.31 0.07 0.1 0.11 0.05 0.06 0.08
Endoplasmic Reticulum 0.06 0.06 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0.05 0
Endosome 0 0.06 0 0 0 0.1 0 0.05 0 – 0 0 0.06 0.1 0.13 0.08 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Mitochondria 0.16 0.09 0 0 0 0.12 0.2 0.15 0.42 – 0.48 0.35 0.1 0 0 0 0 0 0 0.05 0.14 0.05 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0.14 0 0.08 0.05 0.12 0 0 0.05 – 0 0 0.23 0.13 0.16 0.11 0.16 0.13 0 0 0 0 0 0
SpindlePole 0 0 0.21 0.21 0.27 0.2 0.17 0.16 0.09 – 0 0.1 0 0 0 0.17 0.21 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0.05 0.09 0.05 – 0 0.05 0 0 0 0.08 0.05 0.06 0 0.05 0.05 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 82 68 50 44 63 17 43 46 18 – 20 19 66 59 31 135 64 78 91 56 49 31 82 128
Bud 51 41 40 48 52 64 74 63 51 – 51 55 40 50 55 47 27 26 57 53 52 50 96 108
Bud Neck 1 0 2 3 3 5 9 11 5 – 4 9 0 0 0 0 11 0 51 53 54 32 42 39
Bud Site 23 34 20 25 40 48 34 22 15 – 12 15 36 5 5 20 12 11 – – – – – –
Cell Periphery 6 6 9 11 5 10 11 14 5 – 10 15 9 23 43 33 14 11 4 2 4 0 3 2
Cytoplasm 51 55 97 78 111 150 220 272 125 – 145 164 73 250 242 114 52 64 17 23 27 7 17 29
Endoplasmic Reticulum 11 11 0 1 1 0 6 2 1 – 3 2 9 18 13 23 5 9 6 2 3 2 14 12
Endosome 4 11 4 5 5 39 15 28 10 – 11 4 12 39 53 31 10 6 4 10 3 4 5 7
Golgi 0 1 0 1 0 0 0 0 0 – 0 0 0 0 0 20 14 11 1 2 0 1 4 1
Mitochondria 32 17 3 6 10 46 97 78 128 – 172 121 21 6 8 14 7 9 6 11 35 7 20 16
Nucleus 2 1 4 5 9 12 11 13 8 – 7 4 0 1 1 4 4 1 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0 0 1 0 0 0 1
Nucleolus 0 0 1 0 2 7 5 10 10 – 3 1 0 0 1 1 5 1 0 0 0 0 0 0
Peroxisomes 31 26 9 16 13 46 15 12 14 – 11 2 48 51 62 45 34 27 1 1 1 0 3 0
SpindlePole 6 6 45 40 75 79 80 84 27 – 11 34 8 8 15 68 45 32 1 2 4 2 2 3
Vac/Vac Membrane 2 1 10 4 7 16 24 49 14 – 15 19 3 2 1 34 10 13 3 11 13 8 5 18
Unique Cell Count 196 190 210 191 277 386 477 537 305 356 350 212 394 397 404 219 206 251 236 252 153 303 377
Labelled Cell Count 302 278 294 287 396 539 644 704 431 476 464 325 512 530 589 315 299 251 236 252 153 303 377


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.4 6.4 6.0 6.5 5.4 5.5 5.9 5.1 – 4.9 5.3 6.6 6.8 6.6 9.4 9.6 9.3 6.3 6.3 6.5
Std Deviation (1e-4) 0.9 1.1 0.9 1.2 1.3 1.2 1.1 1.2 1.4 – 1.0 1.7 0.9 1.0 0.9 1.6 1.5 1.4 1.2 1.2 1.1
Intensity Change (Log2) – – – -0.1 0.02 -0.24 -0.24 -0.12 -0.33 – -0.39 -0.29 0.03 0.07 0.04 0.55 0.58 0.53 -0.03 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -0.3 -7.2 -5.2 -5.6 -5.9 – -6.4 -6.4 1.7 -2.7 -5.5 2.5 1.3 3.1
Bud 1.5 -0.1 -0.8 -1.1 -2.6 -0.7 – -1.5 -1.0 0 -2.1 -1.7 -2.5 -1.9 -1.8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 2.5 0
Bud Site 1.1 1.6 1.1 -1.1 -2.9 -2.0 – -3.1 -2.5 2.3 -4.9 -4.9 -2.2 -1.6 -1.6
Cell Periphery 0.7 -1.6 -1.1 -1.4 -1.2 -1.8 – -0.9 0 0 0.8 2.7 1.8 1.0 0.5
Cytoplasm -1.1 -1.4 -1.7 0 1.1 -1.2 – -1.3 0.2 -2.5 4.1 3.5 -4.4 -4.9 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 3.1 0 3.5 0 0
Endosome 0 0 3.7 0.9 2.0 0.9 – 0.8 0 2.0 3.6 4.6 2.9 1.6 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 3.3 3.7 3.4
Mitochondria 0 1.5 4.5 6.5 5.2 10.4 – 11.7 9.1 3.8 0 0 1.5 0 1.8
Nucleus 0 0.9 0.9 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1.7 0.2 3.1 -0.8 -1.5 0.2 – -0.7 0 5.5 3.4 4.1 2.8 3.9 3.2
SpindlePole -0.1 1.4 -0.3 -1.5 -1.9 -4.0 – -7.1 -3.9 -5.5 -8.0 -6.9 -1.4 -0.2 -1.5
Vacuole -1.5 -1.3 -0.4 0.2 2.0 -0.1 – -0.3 0.3 -2.0 0 0 1.7 -0.1 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8728 3.6035 3.5618 2.6692 2.6538 3.6914 1.6017 1.9335 1.9387 1.9401 1.4532 1.9807 1.7092 2.2271 2.1354 2.2743 1.9766 2.2796
Actin 0.1538 0.064 0.1486 0.246 0.4235 0.1261 0.1762 0.0603 0.2315 0.3307 0.4756 0.1493 0.2768 0.0811 0.2545 0.2394 0.4943 0.1401
Bud 0.0287 0.0234 0.1785 0.0443 0.0452 0.0027 0.0482 0.0369 0.2083 0.0655 0.0254 0.0069 0.0303 0.0323 0.192 0.0639 0.02 0.0102
Bud Neck 0.079 0.0161 0.0103 0.0637 0.2403 0.7462 0.0524 0.0083 0.0236 0.0947 0.1246 0.7008 0.0641 0.007 0.0159 0.0715 0.0599 0.7262
Bud Periphery 0.0959 0.0299 0.392 0.2332 0.1551 0.0071 0.0883 0.0313 0.3324 0.266 0.1179 0.0144 0.0539 0.0314 0.2943 0.3487 0.1072 0.0187
Bud Site 0.2303 0.6487 0.0926 0.0774 0.0401 0.012 0.2325 0.5406 0.0901 0.0084 0.0202 0.0185 0.2368 0.5657 0.0873 0.0101 0.0128 0.0051
Cell Periphery 0.0157 0.01 0.0094 0.0156 0.0151 0.0016 0.0062 0.004 0.0062 0.0047 0.0073 0.0022 0.0118 0.005 0.0039 0.0168 0.0069 0.0041
Cytoplasm 0.1571 0.1124 0.0692 0.0709 0.0315 0.0241 0.224 0.2198 0.0609 0.1286 0.0222 0.0323 0.1835 0.1956 0.0868 0.1079 0.0147 0.0527
Cytoplasmic Foci 0.0511 0.0252 0.0137 0.07 0.0234 0.0309 0.0498 0.0504 0.0073 0.0122 0.0244 0.0318 0.0635 0.034 0.0143 0.0233 0.039 0.0218
Eisosomes 0.0018 0.0003 0.0008 0.0009 0.001 0.0005 0.0007 0.0002 0.0008 0.0006 0.0014 0.0004 0.0021 0.0006 0.0005 0.0007 0.001 0.0002
Endoplasmic Reticulum 0.0031 0.001 0.0009 0.0025 0.0007 0.0004 0.0066 0.0015 0.0014 0.0017 0.0012 0.0005 0.0024 0.0012 0.002 0.0047 0.002 0.0002
Endosome 0.0143 0.0098 0.006 0.026 0.0017 0.0031 0.0268 0.0172 0.0059 0.0233 0.0442 0.0051 0.012 0.0064 0.0084 0.0174 0.0523 0.0034
Golgi 0.0122 0.0112 0.0037 0.0279 0.0096 0.0033 0.0167 0.0057 0.0077 0.023 0.027 0.009 0.0095 0.0029 0.0065 0.0346 0.0869 0.0106
Lipid Particles 0.0123 0.0033 0.0021 0.0031 0.001 0.0029 0.0136 0.0008 0.0011 0.0021 0.0124 0.0025 0.0093 0.0007 0.0044 0.0101 0.0196 0.0005
Mitochondria 0.0091 0.0015 0.0189 0.0085 0.0014 0.0036 0.0085 0.0052 0.0055 0.031 0.0531 0.0017 0.0052 0.0006 0.0036 0.0046 0.0621 0.0014
None 0.0838 0.0189 0.0241 0.073 0.0026 0.0031 0.0152 0.0066 0.0058 0.0015 0.0042 0.0009 0.0186 0.0271 0.0097 0.006 0.0006 0.0005
Nuclear Periphery 0.0013 0.0003 0.0003 0.0007 0.0001 0.0017 0.0044 0.0006 0.0015 0.0004 0.0009 0.0002 0.0014 0.0003 0.0018 0.0019 0.0006 0
Nucleolus 0.0022 0.0003 0.0005 0.002 0.0005 0.001 0.0006 0.0003 0.0002 0.0001 0.0006 0.0002 0.0008 0.0003 0.0003 0.0002 0.0002 0.0001
Nucleus 0.0027 0.001 0.0008 0.0022 0.0003 0.0014 0.0014 0.0009 0.0006 0.0004 0.0008 0.0004 0.0016 0.0009 0.0072 0.0009 0.0004 0.0003
Peroxisomes 0.0199 0.0157 0.0232 0.0129 0.0056 0.0254 0.0176 0.0045 0.0058 0.0016 0.0272 0.0214 0.0059 0.0025 0.0018 0.0228 0.0154 0.0031
Punctate Nuclear 0.017 0.0018 0.0009 0.0013 0.0004 0.0008 0.0028 0.0009 0.0003 0.0002 0.0034 0.0006 0.002 0.0014 0.0013 0.0014 0.0006 0.0003
Vacuole 0.0081 0.005 0.003 0.0165 0.0007 0.0012 0.0061 0.0034 0.0028 0.0023 0.0042 0.0009 0.0077 0.0029 0.003 0.0116 0.0024 0.0005
Vacuole Periphery 0.0007 0.0003 0.0005 0.0014 0.0001 0.0012 0.0014 0.0005 0.0006 0.001 0.0018 0.0002 0.0009 0.0002 0.0006 0.0017 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1879 14.5852 10.8228 14.1975 15.4122 11.6476 14.7471 13.508 14.9186 17.9185
Translational Efficiency 1.6027 1.0866 1.2994 1.0887 1.1135 1.3303 1.0509 1.0947 1.127 1.0908

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1075 1464 788 1500 1308 1769 1341 1596 2383 3233 2129 3096

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 771.89 884.52 1060.61 991.62 786.48 877.69 963.63 1007.54 779.90 880.78 999.52 999.83
Standard Deviation 84.59 124.48 110.63 128.37 95.73 110.48 113.89 122.91 91.16 117.08 122.04 125.84
Intensity Change Log 2 — 0.196499 0.458427 0.361392 — 0.158301 0.293069 0.357355 — 0.177348 0.377343 0.359356

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.096998 0.067296 0.085706 0.169428* 0.095777 0.044845 0.079417 0.119386* 0.096328 0.055011 0.081745 0.143631*
Bud Neck 0.160905 0.110761 0.087520 0.042418 0.125198 0.154411 0.094290 0.052764 0.141306 0.134645 0.091785 0.047751
Bud Site 0.432596* 0.527876* 0.650417* 0.628374* 0.430011* 0.488840* 0.548317* 0.684612* 0.431177* 0.506516* 0.586107* 0.657365*
Cell Periphery 0.024994 0.005593 0.002080 0.006732 0.013024 0.004557 0.003102 0.006786 0.018424 0.005026 0.002724 0.006760
Cytoplasm 0.154908* 0.162176* 0.087445 0.099320 0.181818* 0.184854* 0.146065* 0.090867 0.169679* 0.174585* 0.124368* 0.094962
Cytoplasmic Foci 0.050807 0.053873 0.014631 0.010605 0.062560 0.043117 0.024407 0.008568 0.057258 0.047987 0.020788 0.009555
Eisosomes 0.010452 0.002366 0.000370 0.000969 0.005221 0.002053 0.000864 0.000824 0.007581 0.002195 0.000681 0.000895
Endoplasmic Reticulum 0.000903 0.001461 0.001793 0.000819 0.001494 0.000985 0.000923 0.000368 0.001228 0.001201 0.001245 0.000586
Endosome 0.008664 0.012106 0.003853 0.003651 0.013663 0.013624 0.008255 0.003475 0.011408 0.012936 0.006626 0.003560
Golgi 0.012722 0.016807 0.000556 0.002726 0.020434 0.013575 0.003514 0.002593 0.016955 0.015038 0.002419 0.002657
Lipid Particles 0.002303 0.001754 0.000958 0.000530 0.003559 0.001151 0.003210 0.000397 0.002993 0.001424 0.002376 0.000461
Mitochondria 0.007717 0.006379 0.000403 0.002593 0.009466 0.008131 0.002759 0.005161 0.008677 0.007338 0.001887 0.003917
Mitotic Spindle 0.000525 0.002126 0.002904 0.006557 0.000400 0.002494 0.005566 0.005684 0.000456 0.002327 0.004581 0.006107
None 0.001159 0.000183 0.000083 0.000051 0.000224 0.000114 0.000517 0.000057 0.000646 0.000145 0.000356 0.000054
Nuclear Periphery 0.000013 0.000009 0.000058 0.000046 0.000016 0.000013 0.000176 0.000089 0.000014 0.000011 0.000132 0.000068
Nuclear Periphery Foci 0.000103 0.000126 0.000775 0.000119 0.000173 0.000063 0.000377 0.000153 0.000141 0.000091 0.000524 0.000137
Nucleolus 0.000050 0.000115 0.000042 0.000024 0.000180 0.000051 0.000105 0.000013 0.000121 0.000080 0.000082 0.000018
Nucleus 0.002329 0.001325 0.005623 0.000931 0.002129 0.001430 0.007233 0.000488 0.002219 0.001383 0.006637 0.000702
Peroxisomes 0.024495 0.018365 0.013644 0.012541 0.023968 0.019876 0.026118 0.007246 0.024206 0.019192 0.021501 0.009811
Vacuole 0.006471 0.008115 0.040988 0.011058 0.009647 0.014765 0.043880 0.009157 0.008215 0.011754 0.042810 0.010078
Vacuole Periphery 0.000885 0.001189 0.000151 0.000509 0.001038 0.001053 0.000907 0.001311 0.000969 0.001115 0.000627 0.000922

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.97 2.06 -6.96 -11.12 -8.54 8.04 2.39 -2.57 -10.14 -4.80 8.51 3.18 -6.86 -15.18 -9.66
Bud Neck 5.60 9.03 15.55 11.69 6.44 -3.51 4.23 10.72 16.08 6.83 1.10 9.01 18.49 19.93 9.57
Bud Site -7.16 -10.37 -14.05 -7.53 -1.70 -4.85 -7.77 -19.88 -16.85 -11.17 -8.39 -12.30 -23.96 -17.27 -9.53
Cell Periphery 8.74 10.89 7.93 -0.82 -4.63 5.15 6.05 3.53 -2.32 -3.92 9.90 11.90 8.16 -2.16 -5.76
Cytoplasm -0.85 5.06 5.98 7.44 0.11 -0.36 3.22 11.16 12.44 7.38 -0.82 5.10 12.28 14.20 6.12
Cytoplasmic Foci -0.70 9.45 11.13 12.67 2.62 4.80 9.64 15.35 12.98 7.46 3.04 12.94 18.84 18.05 7.64
Eisosomes 7.42 9.65 9.06 4.43 -6.16 4.75 7.03 7.21 4.22 0.17 8.76 11.79 11.51 6.08 -1.93
Endoplasmic Reticulum -1.31 -4.45 0.13 1.17 3.41 1.52 2.13 4.64 2.13 3.13 0.10 -1.30 2.78 2.05 3.87
Endosome -2.34 3.54 4.49 6.25 0.40 0.02 3.81 8.14 8.50 4.36 -1.45 4.76 9.19 10.46 3.89
Golgi -2.14 9.76 7.44 9.12 -4.07 3.46 9.68 10.53 8.61 0.97 1.37 12.80 12.86 12.54 -0.45
Lipid Particles 1.16 2.45 4.26 3.77 3.10 3.78 0.31 4.96 3.83 3.85 3.84 0.76 6.34 5.25 4.29
Mitochondria 0.99 7.52 4.58 3.44 -3.64 0.95 4.77 2.54 1.99 -1.56 1.36 7.33 4.59 3.65 -2.25
Mitotic Spindle -1.81 -4.19 -4.38 -2.81 -1.88 -3.82 -4.42 -4.47 -2.58 0.19 -3.74 -5.52 -6.26 -3.82 -0.79
None 1.08 1.14 1.23 1.70 2.90 3.09 -0.96 4.83 2.97 1.52 1.23 0.62 1.45 2.45 1.68
Nuclear Periphery 1.23 -7.08 -1.49 -1.65 3.32 0.70 -1.64 -1.29 -1.35 0.77 1.21 -2.43 -1.74 -1.85 1.38
Nuclear Periphery Foci -1.00 -5.09 -0.75 -0.02 4.91 2.09 -2.40 -0.05 -1.94 2.27 1.57 -5.64 -0.27 -1.69 5.41
Nucleolus -0.96 -0.75 1.73 1.27 2.31 0.93 0.55 1.16 2.68 5.69 0.51 0.46 1.28 1.80 5.81
Nucleus 3.01 -6.49 4.15 0.52 7.22 2.21 -5.87 4.83 5.18 7.65 3.62 -8.73 6.22 3.09 10.42
Peroxisomes 2.34 4.50 4.71 2.92 0.17 1.68 -0.61 7.99 6.99 7.25 2.81 1.49 8.83 6.95 6.25
Vacuole -1.66 -11.20 -4.76 -3.81 9.53 -3.29 -10.52 -1.95 0.70 9.04 -3.67 -15.40 -4.62 -1.79 13.02
Vacuole Periphery -1.05 4.80 1.23 1.85 -1.24 -0.12 0.33 -0.55 -0.51 -0.64 -0.97 1.23 0.17 0.65 -0.76
Standard name
Human Ortholog
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
Localization
Cell Percentages actin (38%), cytoplasm (8%), mixed (24%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00045)
actin - MA (7.1e-06)
Subcompartmental Group N/A

Bud6

Bud6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available