Standard name
Human Ortholog
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.42 0.36 0.24 0.23 0.23 0 0.09 0.09 0.06 – 0.06 0.05 0.31 0.15 0.08 0.33 0.29 0.38 0.36 0.24 0.2 0.21 0.27 0.34
Bud 0.26 0.22 0.19 0.25 0.19 0.17 0.16 0.12 0.17 – 0.14 0.16 0.19 0.13 0.14 0.12 0.12 0.13 0.23 0.23 0.21 0.33 0.32 0.29
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.2 0.23 0.22 0.21 0.14 0.11
Bud Site 0.12 0.18 0.1 0.13 0.14 0.12 0.07 0 0.05 – 0 0 0.17 0 0 0.05 0.05 0.05 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0.06 0.11 0.08 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.26 0.29 0.46 0.41 0.4 0.39 0.46 0.51 0.41 – 0.41 0.47 0.34 0.63 0.61 0.28 0.24 0.31 0.07 0.1 0.11 0.05 0.06 0.08
Endoplasmic Reticulum 0.06 0.06 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0.05 0
Endosome 0 0.06 0 0 0 0.1 0 0.05 0 – 0 0 0.06 0.1 0.13 0.08 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Mitochondria 0.16 0.09 0 0 0 0.12 0.2 0.15 0.42 – 0.48 0.35 0.1 0 0 0 0 0 0 0.05 0.14 0.05 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0.14 0 0.08 0.05 0.12 0 0 0.05 – 0 0 0.23 0.13 0.16 0.11 0.16 0.13 0 0 0 0 0 0
SpindlePole 0 0 0.21 0.21 0.27 0.2 0.17 0.16 0.09 – 0 0.1 0 0 0 0.17 0.21 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0.05 0.09 0.05 – 0 0.05 0 0 0 0.08 0.05 0.06 0 0.05 0.05 0.05 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 82 68 50 44 63 17 43 46 18 – 20 19 66 59 31 135 64 78 91 56 49 31 82 128
Bud 51 41 40 48 52 64 74 63 51 – 51 55 40 50 55 47 27 26 57 53 52 50 96 108
Bud Neck 1 0 2 3 3 5 9 11 5 – 4 9 0 0 0 0 11 0 51 53 54 32 42 39
Bud Site 23 34 20 25 40 48 34 22 15 – 12 15 36 5 5 20 12 11 – – – – – –
Cell Periphery 6 6 9 11 5 10 11 14 5 – 10 15 9 23 43 33 14 11 4 2 4 0 3 2
Cytoplasm 51 55 97 78 111 150 220 272 125 – 145 164 73 250 242 114 52 64 17 23 27 7 17 29
Endoplasmic Reticulum 11 11 0 1 1 0 6 2 1 – 3 2 9 18 13 23 5 9 6 2 3 2 14 12
Endosome 4 11 4 5 5 39 15 28 10 – 11 4 12 39 53 31 10 6 4 10 3 4 5 7
Golgi 0 1 0 1 0 0 0 0 0 – 0 0 0 0 0 20 14 11 1 2 0 1 4 1
Mitochondria 32 17 3 6 10 46 97 78 128 – 172 121 21 6 8 14 7 9 6 11 35 7 20 16
Nucleus 2 1 4 5 9 12 11 13 8 – 7 4 0 1 1 4 4 1 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 1 0 0 1 0 0 0 1
Nucleolus 0 0 1 0 2 7 5 10 10 – 3 1 0 0 1 1 5 1 0 0 0 0 0 0
Peroxisomes 31 26 9 16 13 46 15 12 14 – 11 2 48 51 62 45 34 27 1 1 1 0 3 0
SpindlePole 6 6 45 40 75 79 80 84 27 – 11 34 8 8 15 68 45 32 1 2 4 2 2 3
Vac/Vac Membrane 2 1 10 4 7 16 24 49 14 – 15 19 3 2 1 34 10 13 3 11 13 8 5 18
Unique Cell Count 196 190 210 191 277 386 477 537 305 356 350 212 394 397 404 219 206 251 236 252 153 303 377
Labelled Cell Count 302 278 294 287 396 539 644 704 431 476 464 325 512 530 589 315 299 251 236 252 153 303 377


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.4 6.4 6.0 6.5 5.4 5.5 5.9 5.1 – 4.9 5.3 6.6 6.8 6.6 9.4 9.6 9.3 6.3 6.3 6.5
Std Deviation (1e-4) 0.9 1.1 0.9 1.2 1.3 1.2 1.1 1.2 1.4 – 1.0 1.7 0.9 1.0 0.9 1.6 1.5 1.4 1.2 1.2 1.1
Intensity Change (Log2) – – – -0.1 0.02 -0.24 -0.24 -0.12 -0.33 – -0.39 -0.29 0.03 0.07 0.04 0.55 0.58 0.53 -0.03 -0.03 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -0.3 -7.2 -5.2 -5.6 -5.9 – -6.4 -6.4 1.7 -2.7 -5.5 2.5 1.3 3.1
Bud 1.5 -0.1 -0.8 -1.1 -2.6 -0.7 – -1.5 -1.0 0 -2.1 -1.7 -2.5 -1.9 -1.8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 2.5 0
Bud Site 1.1 1.6 1.1 -1.1 -2.9 -2.0 – -3.1 -2.5 2.3 -4.9 -4.9 -2.2 -1.6 -1.6
Cell Periphery 0.7 -1.6 -1.1 -1.4 -1.2 -1.8 – -0.9 0 0 0.8 2.7 1.8 1.0 0.5
Cytoplasm -1.1 -1.4 -1.7 0 1.1 -1.2 – -1.3 0.2 -2.5 4.1 3.5 -4.4 -4.9 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 3.1 0 3.5 0 0
Endosome 0 0 3.7 0.9 2.0 0.9 – 0.8 0 2.0 3.6 4.6 2.9 1.6 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 3.3 3.7 3.4
Mitochondria 0 1.5 4.5 6.5 5.2 10.4 – 11.7 9.1 3.8 0 0 1.5 0 1.8
Nucleus 0 0.9 0.9 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 1.7 0.2 3.1 -0.8 -1.5 0.2 – -0.7 0 5.5 3.4 4.1 2.8 3.9 3.2
SpindlePole -0.1 1.4 -0.3 -1.5 -1.9 -4.0 – -7.1 -3.9 -5.5 -8.0 -6.9 -1.4 -0.2 -1.5
Vacuole -1.5 -1.3 -0.4 0.2 2.0 -0.1 – -0.3 0.3 -2.0 0 0 1.7 -0.1 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8728 3.6035 3.5618 2.6692 2.6538 3.6914 1.6017 1.9335 1.9387 1.9401 1.4532 1.9807 1.7092 2.2271 2.1354 2.2743 1.9766 2.2796
Actin 0.1538 0.064 0.1486 0.246 0.4235 0.1261 0.1762 0.0603 0.2315 0.3307 0.4756 0.1493 0.2768 0.0811 0.2545 0.2394 0.4943 0.1401
Bud 0.0287 0.0234 0.1785 0.0443 0.0452 0.0027 0.0482 0.0369 0.2083 0.0655 0.0254 0.0069 0.0303 0.0323 0.192 0.0639 0.02 0.0102
Bud Neck 0.079 0.0161 0.0103 0.0637 0.2403 0.7462 0.0524 0.0083 0.0236 0.0947 0.1246 0.7008 0.0641 0.007 0.0159 0.0715 0.0599 0.7262
Bud Periphery 0.0959 0.0299 0.392 0.2332 0.1551 0.0071 0.0883 0.0313 0.3324 0.266 0.1179 0.0144 0.0539 0.0314 0.2943 0.3487 0.1072 0.0187
Bud Site 0.2303 0.6487 0.0926 0.0774 0.0401 0.012 0.2325 0.5406 0.0901 0.0084 0.0202 0.0185 0.2368 0.5657 0.0873 0.0101 0.0128 0.0051
Cell Periphery 0.0157 0.01 0.0094 0.0156 0.0151 0.0016 0.0062 0.004 0.0062 0.0047 0.0073 0.0022 0.0118 0.005 0.0039 0.0168 0.0069 0.0041
Cytoplasm 0.1571 0.1124 0.0692 0.0709 0.0315 0.0241 0.224 0.2198 0.0609 0.1286 0.0222 0.0323 0.1835 0.1956 0.0868 0.1079 0.0147 0.0527
Cytoplasmic Foci 0.0511 0.0252 0.0137 0.07 0.0234 0.0309 0.0498 0.0504 0.0073 0.0122 0.0244 0.0318 0.0635 0.034 0.0143 0.0233 0.039 0.0218
Eisosomes 0.0018 0.0003 0.0008 0.0009 0.001 0.0005 0.0007 0.0002 0.0008 0.0006 0.0014 0.0004 0.0021 0.0006 0.0005 0.0007 0.001 0.0002
Endoplasmic Reticulum 0.0031 0.001 0.0009 0.0025 0.0007 0.0004 0.0066 0.0015 0.0014 0.0017 0.0012 0.0005 0.0024 0.0012 0.002 0.0047 0.002 0.0002
Endosome 0.0143 0.0098 0.006 0.026 0.0017 0.0031 0.0268 0.0172 0.0059 0.0233 0.0442 0.0051 0.012 0.0064 0.0084 0.0174 0.0523 0.0034
Golgi 0.0122 0.0112 0.0037 0.0279 0.0096 0.0033 0.0167 0.0057 0.0077 0.023 0.027 0.009 0.0095 0.0029 0.0065 0.0346 0.0869 0.0106
Lipid Particles 0.0123 0.0033 0.0021 0.0031 0.001 0.0029 0.0136 0.0008 0.0011 0.0021 0.0124 0.0025 0.0093 0.0007 0.0044 0.0101 0.0196 0.0005
Mitochondria 0.0091 0.0015 0.0189 0.0085 0.0014 0.0036 0.0085 0.0052 0.0055 0.031 0.0531 0.0017 0.0052 0.0006 0.0036 0.0046 0.0621 0.0014
None 0.0838 0.0189 0.0241 0.073 0.0026 0.0031 0.0152 0.0066 0.0058 0.0015 0.0042 0.0009 0.0186 0.0271 0.0097 0.006 0.0006 0.0005
Nuclear Periphery 0.0013 0.0003 0.0003 0.0007 0.0001 0.0017 0.0044 0.0006 0.0015 0.0004 0.0009 0.0002 0.0014 0.0003 0.0018 0.0019 0.0006 0
Nucleolus 0.0022 0.0003 0.0005 0.002 0.0005 0.001 0.0006 0.0003 0.0002 0.0001 0.0006 0.0002 0.0008 0.0003 0.0003 0.0002 0.0002 0.0001
Nucleus 0.0027 0.001 0.0008 0.0022 0.0003 0.0014 0.0014 0.0009 0.0006 0.0004 0.0008 0.0004 0.0016 0.0009 0.0072 0.0009 0.0004 0.0003
Peroxisomes 0.0199 0.0157 0.0232 0.0129 0.0056 0.0254 0.0176 0.0045 0.0058 0.0016 0.0272 0.0214 0.0059 0.0025 0.0018 0.0228 0.0154 0.0031
Punctate Nuclear 0.017 0.0018 0.0009 0.0013 0.0004 0.0008 0.0028 0.0009 0.0003 0.0002 0.0034 0.0006 0.002 0.0014 0.0013 0.0014 0.0006 0.0003
Vacuole 0.0081 0.005 0.003 0.0165 0.0007 0.0012 0.0061 0.0034 0.0028 0.0023 0.0042 0.0009 0.0077 0.0029 0.003 0.0116 0.0024 0.0005
Vacuole Periphery 0.0007 0.0003 0.0005 0.0014 0.0001 0.0012 0.0014 0.0005 0.0006 0.001 0.0018 0.0002 0.0009 0.0002 0.0006 0.0017 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1879 14.5852 10.8228 14.1975 15.4122 11.6476 14.7471 13.508 14.9186 17.9185
Translational Efficiency 1.6027 1.0866 1.2994 1.0887 1.1135 1.3303 1.0509 1.0947 1.127 1.0908

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1075 1464 788 1500 1308 1769 1341 1596 2383 3233 2129 3096

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 771.89 884.52 1060.61 991.62 786.48 877.69 963.63 1007.54 779.90 880.78 999.52 999.83
Standard Deviation 84.59 124.48 110.63 128.37 95.73 110.48 113.89 122.91 91.16 117.08 122.04 125.84
Intensity Change Log 2 — 0.196499 0.458427 0.361392 — 0.158301 0.293069 0.357355 — 0.177348 0.377343 0.359356

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.096998 0.067296 0.085706 0.169428* 0.095777 0.044845 0.079417 0.119386* 0.096328 0.055011 0.081745 0.143631*
Bud Neck 0.160905 0.110761 0.087520 0.042418 0.125198 0.154411 0.094290 0.052764 0.141306 0.134645 0.091785 0.047751
Bud Site 0.432596* 0.527876* 0.650417* 0.628374* 0.430011* 0.488840* 0.548317* 0.684612* 0.431177* 0.506516* 0.586107* 0.657365*
Cell Periphery 0.024994 0.005593 0.002080 0.006732 0.013024 0.004557 0.003102 0.006786 0.018424 0.005026 0.002724 0.006760
Cytoplasm 0.154908* 0.162176* 0.087445 0.099320 0.181818* 0.184854* 0.146065* 0.090867 0.169679* 0.174585* 0.124368* 0.094962
Cytoplasmic Foci 0.050807 0.053873 0.014631 0.010605 0.062560 0.043117 0.024407 0.008568 0.057258 0.047987 0.020788 0.009555
Eisosomes 0.010452 0.002366 0.000370 0.000969 0.005221 0.002053 0.000864 0.000824 0.007581 0.002195 0.000681 0.000895
Endoplasmic Reticulum 0.000903 0.001461 0.001793 0.000819 0.001494 0.000985 0.000923 0.000368 0.001228 0.001201 0.001245 0.000586
Endosome 0.008664 0.012106 0.003853 0.003651 0.013663 0.013624 0.008255 0.003475 0.011408 0.012936 0.006626 0.003560
Golgi 0.012722 0.016807 0.000556 0.002726 0.020434 0.013575 0.003514 0.002593 0.016955 0.015038 0.002419 0.002657
Lipid Particles 0.002303 0.001754 0.000958 0.000530 0.003559 0.001151 0.003210 0.000397 0.002993 0.001424 0.002376 0.000461
Mitochondria 0.007717 0.006379 0.000403 0.002593 0.009466 0.008131 0.002759 0.005161 0.008677 0.007338 0.001887 0.003917
Mitotic Spindle 0.000525 0.002126 0.002904 0.006557 0.000400 0.002494 0.005566 0.005684 0.000456 0.002327 0.004581 0.006107
None 0.001159 0.000183 0.000083 0.000051 0.000224 0.000114 0.000517 0.000057 0.000646 0.000145 0.000356 0.000054
Nuclear Periphery 0.000013 0.000009 0.000058 0.000046 0.000016 0.000013 0.000176 0.000089 0.000014 0.000011 0.000132 0.000068
Nuclear Periphery Foci 0.000103 0.000126 0.000775 0.000119 0.000173 0.000063 0.000377 0.000153 0.000141 0.000091 0.000524 0.000137
Nucleolus 0.000050 0.000115 0.000042 0.000024 0.000180 0.000051 0.000105 0.000013 0.000121 0.000080 0.000082 0.000018
Nucleus 0.002329 0.001325 0.005623 0.000931 0.002129 0.001430 0.007233 0.000488 0.002219 0.001383 0.006637 0.000702
Peroxisomes 0.024495 0.018365 0.013644 0.012541 0.023968 0.019876 0.026118 0.007246 0.024206 0.019192 0.021501 0.009811
Vacuole 0.006471 0.008115 0.040988 0.011058 0.009647 0.014765 0.043880 0.009157 0.008215 0.011754 0.042810 0.010078
Vacuole Periphery 0.000885 0.001189 0.000151 0.000509 0.001038 0.001053 0.000907 0.001311 0.000969 0.001115 0.000627 0.000922

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.97 2.06 -6.96 -11.12 -8.54 8.04 2.39 -2.57 -10.14 -4.80 8.51 3.18 -6.86 -15.18 -9.66
Bud Neck 5.60 9.03 15.55 11.69 6.44 -3.51 4.23 10.72 16.08 6.83 1.10 9.01 18.49 19.93 9.57
Bud Site -7.16 -10.37 -14.05 -7.53 -1.70 -4.85 -7.77 -19.88 -16.85 -11.17 -8.39 -12.30 -23.96 -17.27 -9.53
Cell Periphery 8.74 10.89 7.93 -0.82 -4.63 5.15 6.05 3.53 -2.32 -3.92 9.90 11.90 8.16 -2.16 -5.76
Cytoplasm -0.85 5.06 5.98 7.44 0.11 -0.36 3.22 11.16 12.44 7.38 -0.82 5.10 12.28 14.20 6.12
Cytoplasmic Foci -0.70 9.45 11.13 12.67 2.62 4.80 9.64 15.35 12.98 7.46 3.04 12.94 18.84 18.05 7.64
Eisosomes 7.42 9.65 9.06 4.43 -6.16 4.75 7.03 7.21 4.22 0.17 8.76 11.79 11.51 6.08 -1.93
Endoplasmic Reticulum -1.31 -4.45 0.13 1.17 3.41 1.52 2.13 4.64 2.13 3.13 0.10 -1.30 2.78 2.05 3.87
Endosome -2.34 3.54 4.49 6.25 0.40 0.02 3.81 8.14 8.50 4.36 -1.45 4.76 9.19 10.46 3.89
Golgi -2.14 9.76 7.44 9.12 -4.07 3.46 9.68 10.53 8.61 0.97 1.37 12.80 12.86 12.54 -0.45
Lipid Particles 1.16 2.45 4.26 3.77 3.10 3.78 0.31 4.96 3.83 3.85 3.84 0.76 6.34 5.25 4.29
Mitochondria 0.99 7.52 4.58 3.44 -3.64 0.95 4.77 2.54 1.99 -1.56 1.36 7.33 4.59 3.65 -2.25
Mitotic Spindle -1.81 -4.19 -4.38 -2.81 -1.88 -3.82 -4.42 -4.47 -2.58 0.19 -3.74 -5.52 -6.26 -3.82 -0.79
None 1.08 1.14 1.23 1.70 2.90 3.09 -0.96 4.83 2.97 1.52 1.23 0.62 1.45 2.45 1.68
Nuclear Periphery 1.23 -7.08 -1.49 -1.65 3.32 0.70 -1.64 -1.29 -1.35 0.77 1.21 -2.43 -1.74 -1.85 1.38
Nuclear Periphery Foci -1.00 -5.09 -0.75 -0.02 4.91 2.09 -2.40 -0.05 -1.94 2.27 1.57 -5.64 -0.27 -1.69 5.41
Nucleolus -0.96 -0.75 1.73 1.27 2.31 0.93 0.55 1.16 2.68 5.69 0.51 0.46 1.28 1.80 5.81
Nucleus 3.01 -6.49 4.15 0.52 7.22 2.21 -5.87 4.83 5.18 7.65 3.62 -8.73 6.22 3.09 10.42
Peroxisomes 2.34 4.50 4.71 2.92 0.17 1.68 -0.61 7.99 6.99 7.25 2.81 1.49 8.83 6.95 6.25
Vacuole -1.66 -11.20 -4.76 -3.81 9.53 -3.29 -10.52 -1.95 0.70 9.04 -3.67 -15.40 -4.62 -1.79 13.02
Vacuole Periphery -1.05 4.80 1.23 1.85 -1.24 -0.12 0.33 -0.55 -0.51 -0.64 -0.97 1.23 0.17 0.65 -0.76
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Actin- and formin-interacting protein; participates in actin cable assembly and organization as a nucleation-promoting factor (NPF) for formins Bni1p and Bnr1p; a triple helical coiled-coil domain in the C-terminal region interacts with Bni1p; involved in polarized cell growth; isolated as bipolar budding mutant; potential Cdc28p substrate
Localization
Cell Percentages actin (38%), cytoplasm (8%), mixed (24%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00045)
actin - MA (7.1e-06)
Subcompartmental Group N/A

Bud6

Bud6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available