ORF
Human Ortholog
Description Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; predicted to be palmitoylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.07 0.13 0.12 0.14 0.16 0.09 0.09 0.05 0.08 0.1 0 0 0 0.26 0.23 0.23 0.14 0.13 0.13 0.1 0.09 0.08
Bud 0 0 0 0.06 0 0.14 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.05 0.06 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.25 0.17 0.38 0.28 0.22 0.12 0.16 0.23 0.15 0.22 0.19 0.26 0.32 0.37 0.51 0.58 0.42 0.46 0.4 0.57 0.42 0.38 0.28
Cytoplasm 0.75 0.87 0.63 0.75 0.8 0.73 0.8 0.72 0.81 0.78 0.7 0.9 0.87 0.85 0.26 0.26 0.43 0.13 0.23 0.09 0.23 0.29 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.08 0 0.08 0.07 0 0 0.06 0.07 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.09 0.27 0.19 0.19 0.13 0 0 0 0.11 0.11 0.07 0.06 0 0.08 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 38 17 18 20 29 20 24 11 19 20 10 22 17 62 49 44 37 74 23 32 29 33
Bud 5 14 5 9 3 25 6 13 8 12 4 3 2 2 5 5 5 2 12 1 8 7 12
Bud Neck 4 19 0 2 3 4 1 0 0 0 1 5 2 2 0 0 0 20 55 9 21 16 24
Bud Site 0 0 0 0 0 6 1 1 0 0 1 0 0 0 0 0 0
Cell Periphery 56 91 50 41 31 21 37 58 36 53 40 96 160 164 122 123 81 126 230 105 140 127 115
Cytoplasm 167 455 83 112 115 131 184 184 191 184 148 326 436 373 61 56 84 34 132 17 78 97 143
Endoplasmic Reticulum 3 3 1 2 3 0 7 1 0 2 10 3 1 4 19 9 15 19 12 5 18 23 30
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3 3 3 4 0 4 3 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 2 0 19 29 5 1 7 0 3 2 1
Mitochondria 6 4 0 4 2 24 21 68 44 45 28 1 2 1 26 24 13 15 21 14 11 15 11
Nucleus 0 1 0 0 1 1 3 2 2 1 3 0 1 0 3 3 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4 0 0 0 0 0 0 0 0 0 1 0 2 0 0 2 0 1 0 0 0 0 0
SpindlePole 5 3 4 2 2 3 3 3 1 2 4 4 2 6 9 5 5 1 0 0 2 2 1
Vac/Vac Membrane 1 1 0 0 0 0 3 1 2 0 0 0 1 1 14 8 17 5 12 4 6 3 13
Unique Cell Count 223 523 131 149 144 180 229 255 236 236 210 363 501 438 239 212 195 275 579 185 334 334 405
Labelled Cell Count 273 630 160 191 181 244 286 355 295 318 261 449 633 570 343 316 274 275 579 185 334 334 405


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.2 5.8 5.4 6.1 4.6 5.6 5.3 5.9 5.7 5.9 5.7 6.1 6.4 11.6 12.1 10.9 5.4 5.6 5.6
Std Deviation (1e-4) 1.7 1.1 1.0 1.2 1.3 1.1 1.3 1.3 1.7 1.3 1.3 1.1 3.9 1.7 2.4 2.3 2.4 2.1 2.0 2.1
Intensity Change (Log2) -0.08 0.08 -0.34 -0.04 -0.12 0.04 -0.02 0.03 -0.02 0.09 0.16 1.0 1.07 0.92 -0.09 -0.05 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 0.2 0 -1.3 -1.1 -2.9 -1.5 -1.0 -4.4 -3.6 -3.9 2.9 2.3 2.2
Bud 0.9 0 0 0 0.6 0 0.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 -3.0 0 -4.7 -3.2 -5.0 -3.2 -3.9 -2.5 -1.3 -0.2 2.4 3.6 0.6
Cytoplasm 2.1 3.0 1.8 3.5 1.8 3.7 3.0 1.4 6.9 6.3 5.5 -7.1 -6.8 -3.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.9 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.3 4.4 0
Mitochondria 0 0 0 3.6 6.5 5.3 5.3 4.4 0 0 0 3.9 4.0 3.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5029 0.4901 -0.1084 0.0344 0.0194 0.1134 2.019 1.6451 1.9853 1.4444 1.5127 1.7798 3.3425 4.7013 4.9408 4.3083 4.6445 4.3742
Actin 0.0243 0.022 0.0112 0.0147 0.003 0.0079 0.0498 0.0052 0.0235 0.0155 0.0205 0.0052 0.0013 0.0025 0.0031 0.0049 0.0052 0.0019
Bud 0.0036 0.0042 0.0074 0.0033 0.0019 0.0014 0.0031 0.0028 0.0021 0.0018 0.0016 0.0004 0.0011 0.0037 0.0004 0.0007 0.0006 0.0005
Bud Neck 0.0072 0.0126 0.0156 0.0083 0.0368 0.0485 0.0065 0.002 0.0089 0.0036 0.0063 0.038 0.0136 0.0099 0.0073 0.0106 0.0317 0.0343
Bud Periphery 0.0071 0.0112 0.0204 0.0359 0.0173 0.0076 0.0079 0.0086 0.0063 0.0073 0.0079 0.0063 0.0046 0.002 0.0037 0.005 0.005 0.0045
Bud Site 0.0357 0.1929 0.0724 0.0259 0.0034 0.0041 0.0224 0.0709 0.0595 0.0052 0.0085 0.0007 0.0189 0.1014 0.0235 0.0033 0.0053 0.001
Cell Periphery 0.0725 0.0865 0.0871 0.0588 0.0818 0.0871 0.0798 0.058 0.0486 0.0331 0.0902 0.0721 0.1024 0.1103 0.0969 0.0491 0.0896 0.0568
Cytoplasm 0.396 0.4071 0.4599 0.4254 0.5133 0.4818 0.3448 0.5624 0.5131 0.6038 0.6656 0.5995 0.5391 0.5075 0.543 0.5986 0.6355 0.5804
Cytoplasmic Foci 0.0216 0.0091 0.0066 0.0067 0.0104 0.0099 0.0581 0.0043 0.0213 0.0091 0.0076 0.0047 0.0036 0.005 0.0019 0.0063 0.0015 0.0012
Eisosomes 0.0053 0.0023 0.0027 0.0017 0.0008 0.0013 0.001 0.0009 0.0009 0.0003 0.0007 0.0018 0.0024 0.0013 0.0016 0.0008 0.0005 0.0005
Endoplasmic Reticulum 0.0059 0.0043 0.0058 0.0052 0.0055 0.0033 0.0133 0.0037 0.0038 0.0089 0.0068 0.0039 0.0057 0.0119 0.0038 0.0074 0.0145 0.0021
Endosome 0.0066 0.0007 0.0014 0.0012 0.0231 0.0008 0.0371 0.0007 0.0265 0.0226 0.0016 0.0011 0.0005 0.0005 0.0002 0.0007 0.0004 0.0001
Golgi 0.0023 0.0009 0.0007 0.0007 0.0007 0.0005 0.0089 0.0004 0.014 0.0093 0.0018 0.0004 0.0004 0.0002 0.0003 0.0005 0.0004 0.0001
Lipid Particles 0.0045 0.001 0.0014 0.0011 0.0016 0.0013 0.0182 0.0008 0.0103 0.0017 0.0051 0.0015 0.0013 0.0096 0.0009 0.002 0.001 0.0002
Mitochondria 0.0014 0.0012 0.0004 0.0006 0.0007 0.0003 0.0061 0.0002 0.0082 0.0042 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001
None 0.3891 0.2316 0.2965 0.3952 0.2866 0.3377 0.257 0.274 0.2379 0.2659 0.1699 0.2561 0.2979 0.2257 0.3107 0.3039 0.198 0.3143
Nuclear Periphery 0.0012 0.0004 0.0004 0.0009 0.001 0.0003 0.028 0.0004 0.0008 0.0005 0.0006 0.0003 0.0003 0.0003 0.0002 0.0003 0.0004 0.0002
Nucleolus 0.0019 0.0009 0.0008 0.0013 0.0004 0.0006 0.0035 0.0004 0.0005 0.0002 0.0002 0.0002 0.0017 0.0006 0.0002 0.0004 0.0001 0.0001
Nucleus 0.0037 0.0025 0.0018 0.0024 0.0006 0.001 0.0109 0.0008 0.0009 0.0008 0.0005 0.0005 0.0015 0.0011 0.0004 0.0007 0.0004 0.0005
Peroxisomes 0.0014 0.0004 0.0004 0.0004 0.0002 0.0003 0.0222 0.0001 0.0089 0.0004 0.0003 0.0001 0.0001 0 0 0.0001 0 0
Punctate Nuclear 0.0037 0.0056 0.002 0.0025 0.0007 0.0015 0.0098 0.002 0.0013 0.0003 0.002 0.0055 0.0004 0.0007 0.0002 0.0004 0.0001 0.0002
Vacuole 0.0049 0.0026 0.0049 0.0077 0.0061 0.0028 0.0094 0.0015 0.0024 0.005 0.0021 0.0014 0.003 0.0054 0.0017 0.0037 0.01 0.0008
Vacuole Periphery 0.0002 0.0001 0.0002 0.0002 0.0041 0.0001 0.0022 0 0.0005 0.0006 0.0001 0 0 0.0001 0 0.0001 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.7876 9.55 5.0618 10.1462 15.8297 10.6771 11.3803 10.2412 13.1216 18.4994
Translational Efficiency 2.0568 1.6269 1.2149 1.2027 1.3675 2.9999 1.2576 1.3484 1.05 1.059

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2020 1100 1090 1817 319 2464 1514 1587 2339 3564 2604 3404

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.34 721.08 876.01 900.34 674.75 770.99 813.00 959.91 702.03 755.59 839.38 928.11
Standard Deviation 79.66 106.36 125.89 162.52 73.02 95.06 115.37 185.18 79.53 101.34 123.85 175.98
Intensity Change Log 2 0.029797 0.310585 0.350107 0.192359 0.268902 0.508546 0.111508 0.290371 0.429689

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006111 0.005608 0.010897 0.047432 0.000440 0.004131 0.008927 0.036765 0.005338 0.004587 0.009752 0.042459
Bud Neck 0.069465 0.044858 0.051342 0.046546 0.028599 0.077098 0.027818 0.053144 0.063891 0.067147 0.037665 0.049622
Bud Site 0.023040 0.046238 0.118066* 0.170615* 0.005565 0.048520 0.054519 0.164610* 0.020657 0.047816 0.081119 0.167816*
Cell Periphery 0.006057 0.002704 0.032200 0.100080* 0.002723 0.003376 0.029354 0.247512* 0.005603 0.003169 0.030545 0.168816*
Cytoplasm 0.507200* 0.440011* 0.423920* 0.372144* 0.521300* 0.516045* 0.429384* 0.303474* 0.509123* 0.492577* 0.427097* 0.340129*
Cytoplasmic Foci 0.135898 0.252438* 0.047280 0.010598 0.211772* 0.123646 0.041974 0.009329 0.146246 0.163397 0.044195 0.010007
Eisosomes 0.020941 0.004480 0.007691 0.024090 0.004532 0.006649 0.008702 0.034344 0.018703 0.005980 0.008279 0.028871
Endoplasmic Reticulum 0.002726 0.002765 0.009925 0.005261 0.001219 0.001700 0.010406 0.006735 0.002521 0.002028 0.010204 0.005948
Endosome 0.008446 0.024349 0.002248 0.002723 0.007882 0.009960 0.001769 0.001661 0.008369 0.014401 0.001970 0.002228
Golgi 0.013479 0.018104 0.000997 0.002289 0.003691 0.006666 0.000451 0.001743 0.012144 0.010196 0.000679 0.002035
Lipid Particles 0.016658 0.013320 0.003591 0.002073 0.011322 0.007743 0.002875 0.001930 0.015930 0.009465 0.003175 0.002006
Mitochondria 0.007499 0.007380 0.000231 0.001496 0.001446 0.005687 0.000230 0.001057 0.006674 0.006209 0.000230 0.001292
Mitotic Spindle 0.000670 0.001809 0.000169 0.005021 0.000163 0.002221 0.000501 0.001048 0.000601 0.002094 0.000362 0.003169
None 0.001766 0.002470 0.001282 0.000821 0.000702 0.003724 0.002239 0.000758 0.001621 0.003337 0.001838 0.000792
Nuclear Periphery 0.000132 0.000164 0.000184 0.000144 0.000246 0.000130 0.000309 0.000104 0.000148 0.000140 0.000257 0.000125
Nuclear Periphery Foci 0.000316 0.000513 0.002548 0.000637 0.000378 0.000266 0.001829 0.000637 0.000324 0.000342 0.002130 0.000637
Nucleolus 0.000427 0.000571 0.000133 0.000086 0.000358 0.000851 0.000126 0.000046 0.000418 0.000764 0.000129 0.000067
Nucleus 0.124433 0.051969 0.178053* 0.072185 0.147956* 0.108940 0.270427* 0.054138 0.127641 0.091357 0.231760* 0.063772
Peroxisomes 0.012425 0.016328 0.000863 0.001160 0.004073 0.009899 0.000685 0.000966 0.011286 0.011883 0.000760 0.001070
Vacuole 0.041625 0.061921 0.108292 0.133092 0.045177 0.061967 0.107409 0.079034 0.042109 0.061953 0.107779 0.107889
Vacuole Periphery 0.000687 0.002002 0.000089 0.001505 0.000455 0.000782 0.000064 0.000966 0.000655 0.001159 0.000075 0.001253

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.49 -3.77 -15.69 -15.78 -13.33 -9.95 -8.96 -19.81 -17.43 -13.69 1.07 -4.52 -21.68 -23.38 -18.93
Bud Neck 6.69 4.45 7.85 0.52 1.54 -9.32 0.40 -4.64 6.99 -8.58 -1.01 9.11 5.76 7.82 -4.65
Bud Site -6.86 -15.18 -24.94 -18.68 -5.62 -17.01 -14.23 -28.37* -19.25 -17.53 -11.98 -17.15 -35.07 -26.80 -16.76
Cell Periphery 5.67 -9.71 -19.33 -20.18 -11.90 -1.33 -10.30 -31.43* -31.37* -26.95* 5.02 -13.05 -34.98 -35.66* -27.83*
Cytoplasm 7.57 10.22 17.35 7.00 4.94 0.38 7.83 16.65 26.31 12.68 2.77 13.84 26.77 24.92 11.97
Cytoplasmic Foci -16.77 21.41 35.83 39.63 15.33 9.12 18.04 21.83 37.33 15.75 -3.92 28.57 41.21 51.35 21.96
Eisosomes 12.47 9.79 -0.58 -12.39 -9.90 -2.56 -3.50 -15.35 -14.93 -13.39 11.71 9.42 -5.82 -19.03 -16.42
Endoplasmic Reticulum -0.08 -8.65 -4.98 -4.91 5.40 -2.01 -11.11 -9.00 -8.30 3.69 1.83 -12.44 -8.05 -10.29 6.45
Endosome -9.16 11.91 8.87 12.67 -0.54 -1.41 4.38 4.36 13.13 0.43 -7.63 13.33 11.33 17.55 -0.39
Golgi -3.08 19.64 13.24 10.92 -1.86 -3.51 4.18 2.08 7.61 -2.35 2.66 20.54 14.90 13.00 -3.08
Lipid Particles 2.70 14.37 15.39 11.98 4.00 2.91 7.55 8.40 12.33 4.42 7.26 16.13 17.31 16.47 5.32
Mitochondria 0.12 10.27 8.04 5.96 -4.08 -7.04 3.40 1.04 9.17 -5.97 0.62 10.48 8.57 10.41 -6.09
Mitotic Spindle -1.26 1.59 -3.49 -1.96 -4.05 -3.45 -1.17 -1.85 1.60 -1.04 -2.70 0.79 -3.49 -1.14 -4.05
None -1.04 1.50 2.52 2.30 0.99 -5.16 -2.46 -0.18 4.72 2.20 -3.62 -0.30 3.12 5.18 2.36
Nuclear Periphery -0.93 -4.51 -0.97 0.05 1.16 3.48 -2.68 4.25 1.77 14.36 0.40 -9.49 0.58 0.23 7.90
Nuclear Periphery Foci -3.39 -13.70 -5.64 -2.51 11.24 2.33 -11.29 -2.98 -4.41 8.03 -0.60 -17.55 -5.89 -6.21 13.54
Nucleolus -1.44 5.23 5.38 4.81 1.55 -2.26 3.76 5.10 3.79 10.89 -2.28 5.88 6.83 4.65 3.60
Nucleus 18.78 -8.41 11.73 -6.76 16.12 4.62 -12.66 10.84 13.70 33.28 10.20 -20.61 17.58 8.56 35.32
Peroxisomes -2.76 15.54 14.81 12.59 -1.37 -6.72 7.63 6.91 11.78 -2.23 -0.66 16.26 15.64 16.72 -2.42
Vacuole -7.15 -16.13 -23.75 -17.85 -6.28 -4.83 -15.63 -9.13 -6.18 6.36 -10.44 -24.67 -25.30 -18.37 -1.74
Vacuole Periphery -5.65 9.94 -1.46 1.22 -2.76 -3.07 4.26 -0.99 -0.33 -1.82 -5.25 10.93 -1.52 -0.04 -3.27
ORF
Human Ortholog
Description Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; predicted to be palmitoylated
Localization
Cell Percentages cytoplasm (17%), ER (2%), mixed (63%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YLR326W

YLR326W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr326w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available