ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 38 | 17 | 18 | 20 | 29 | 20 | – | 24 | 11 | 19 | 20 | 10 | 22 | 17 | 62 | 49 | 44 | 37 | 74 | 23 | 32 | 29 | 33 |
Bud | 5 | 14 | 5 | 9 | 3 | 25 | 6 | – | 13 | 8 | 12 | 4 | 3 | 2 | 2 | 5 | 5 | 5 | 2 | 12 | 1 | 8 | 7 | 12 |
Bud Neck | 4 | 19 | 0 | 2 | 3 | 4 | 1 | – | 0 | 0 | 0 | 1 | 5 | 2 | 2 | 0 | 0 | 0 | 20 | 55 | 9 | 21 | 16 | 24 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 6 | 1 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 56 | 91 | 50 | 41 | 31 | 21 | 37 | – | 58 | 36 | 53 | 40 | 96 | 160 | 164 | 122 | 123 | 81 | 126 | 230 | 105 | 140 | 127 | 115 |
Cytoplasm | 167 | 455 | 83 | 112 | 115 | 131 | 184 | – | 184 | 191 | 184 | 148 | 326 | 436 | 373 | 61 | 56 | 84 | 34 | 132 | 17 | 78 | 97 | 143 |
Endoplasmic Reticulum | 3 | 3 | 1 | 2 | 3 | 0 | 7 | – | 1 | 0 | 2 | 10 | 3 | 1 | 4 | 19 | 9 | 15 | 19 | 12 | 5 | 18 | 23 | 30 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 3 | 3 | 4 | 0 | 4 | 3 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 19 | 29 | 5 | 1 | 7 | 0 | 3 | 2 | 1 |
Mitochondria | 6 | 4 | 0 | 4 | 2 | 24 | 21 | – | 68 | 44 | 45 | 28 | 1 | 2 | 1 | 26 | 24 | 13 | 15 | 21 | 14 | 11 | 15 | 11 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 3 | – | 2 | 2 | 1 | 3 | 0 | 1 | 0 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 3 | 4 | 2 | 2 | 3 | 3 | – | 3 | 1 | 2 | 4 | 4 | 2 | 6 | 9 | 5 | 5 | 1 | 0 | 0 | 2 | 2 | 1 |
Vac/Vac Membrane | 1 | 1 | 0 | 0 | 0 | 0 | 3 | – | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 14 | 8 | 17 | 5 | 12 | 4 | 6 | 3 | 13 |
Unique Cell Count | 223 | 523 | 131 | 149 | 144 | 180 | 229 | 255 | 236 | 236 | 210 | 363 | 501 | 438 | 239 | 212 | 195 | 275 | 579 | 185 | 334 | 334 | 405 | |
Labelled Cell Count | 273 | 630 | 160 | 191 | 181 | 244 | 286 | 355 | 295 | 318 | 261 | 449 | 633 | 570 | 343 | 316 | 274 | 275 | 579 | 185 | 334 | 334 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 5.2 | 5.8 | 5.4 | 6.1 | 4.6 | 5.6 | – | 5.3 | 5.9 | 5.7 | 5.9 | 5.7 | 6.1 | 6.4 | 11.6 | 12.1 | 10.9 | 5.4 | 5.6 | 5.6 |
Std Deviation (1e-4) | 1.7 | 1.1 | 1.0 | 1.2 | 1.3 | 1.1 | 1.3 | – | 1.3 | 1.7 | 1.3 | 1.3 | 1.1 | 3.9 | 1.7 | 2.4 | 2.3 | 2.4 | 2.1 | 2.0 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.08 | -0.34 | -0.04 | – | -0.12 | 0.04 | -0.02 | 0.03 | -0.02 | 0.09 | 0.16 | 1.0 | 1.07 | 0.92 | -0.09 | -0.05 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | 0.2 | 0 | -1.3 | – | -1.1 | -2.9 | -1.5 | -1.0 | -4.4 | -3.6 | -3.9 | 2.9 | 2.3 | 2.2 |
Bud | 0.9 | 0 | 0 | 0 | – | 0.6 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.9 | -3.0 | 0 | -4.7 | – | -3.2 | -5.0 | -3.2 | -3.9 | -2.5 | -1.3 | -0.2 | 2.4 | 3.6 | 0.6 |
Cytoplasm | 2.1 | 3.0 | 1.8 | 3.5 | – | 1.8 | 3.7 | 3.0 | 1.4 | 6.9 | 6.3 | 5.5 | -7.1 | -6.8 | -3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 4.4 | 0 |
Mitochondria | 0 | 0 | 0 | 3.6 | – | 6.5 | 5.3 | 5.3 | 4.4 | 0 | 0 | 0 | 3.9 | 4.0 | 3.0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5029 | 0.4901 | -0.1084 | 0.0344 | 0.0194 | 0.1134 | 2.019 | 1.6451 | 1.9853 | 1.4444 | 1.5127 | 1.7798 | 3.3425 | 4.7013 | 4.9408 | 4.3083 | 4.6445 | 4.3742 |
Actin | 0.0243 | 0.022 | 0.0112 | 0.0147 | 0.003 | 0.0079 | 0.0498 | 0.0052 | 0.0235 | 0.0155 | 0.0205 | 0.0052 | 0.0013 | 0.0025 | 0.0031 | 0.0049 | 0.0052 | 0.0019 |
Bud | 0.0036 | 0.0042 | 0.0074 | 0.0033 | 0.0019 | 0.0014 | 0.0031 | 0.0028 | 0.0021 | 0.0018 | 0.0016 | 0.0004 | 0.0011 | 0.0037 | 0.0004 | 0.0007 | 0.0006 | 0.0005 |
Bud Neck | 0.0072 | 0.0126 | 0.0156 | 0.0083 | 0.0368 | 0.0485 | 0.0065 | 0.002 | 0.0089 | 0.0036 | 0.0063 | 0.038 | 0.0136 | 0.0099 | 0.0073 | 0.0106 | 0.0317 | 0.0343 |
Bud Periphery | 0.0071 | 0.0112 | 0.0204 | 0.0359 | 0.0173 | 0.0076 | 0.0079 | 0.0086 | 0.0063 | 0.0073 | 0.0079 | 0.0063 | 0.0046 | 0.002 | 0.0037 | 0.005 | 0.005 | 0.0045 |
Bud Site | 0.0357 | 0.1929 | 0.0724 | 0.0259 | 0.0034 | 0.0041 | 0.0224 | 0.0709 | 0.0595 | 0.0052 | 0.0085 | 0.0007 | 0.0189 | 0.1014 | 0.0235 | 0.0033 | 0.0053 | 0.001 |
Cell Periphery | 0.0725 | 0.0865 | 0.0871 | 0.0588 | 0.0818 | 0.0871 | 0.0798 | 0.058 | 0.0486 | 0.0331 | 0.0902 | 0.0721 | 0.1024 | 0.1103 | 0.0969 | 0.0491 | 0.0896 | 0.0568 |
Cytoplasm | 0.396 | 0.4071 | 0.4599 | 0.4254 | 0.5133 | 0.4818 | 0.3448 | 0.5624 | 0.5131 | 0.6038 | 0.6656 | 0.5995 | 0.5391 | 0.5075 | 0.543 | 0.5986 | 0.6355 | 0.5804 |
Cytoplasmic Foci | 0.0216 | 0.0091 | 0.0066 | 0.0067 | 0.0104 | 0.0099 | 0.0581 | 0.0043 | 0.0213 | 0.0091 | 0.0076 | 0.0047 | 0.0036 | 0.005 | 0.0019 | 0.0063 | 0.0015 | 0.0012 |
Eisosomes | 0.0053 | 0.0023 | 0.0027 | 0.0017 | 0.0008 | 0.0013 | 0.001 | 0.0009 | 0.0009 | 0.0003 | 0.0007 | 0.0018 | 0.0024 | 0.0013 | 0.0016 | 0.0008 | 0.0005 | 0.0005 |
Endoplasmic Reticulum | 0.0059 | 0.0043 | 0.0058 | 0.0052 | 0.0055 | 0.0033 | 0.0133 | 0.0037 | 0.0038 | 0.0089 | 0.0068 | 0.0039 | 0.0057 | 0.0119 | 0.0038 | 0.0074 | 0.0145 | 0.0021 |
Endosome | 0.0066 | 0.0007 | 0.0014 | 0.0012 | 0.0231 | 0.0008 | 0.0371 | 0.0007 | 0.0265 | 0.0226 | 0.0016 | 0.0011 | 0.0005 | 0.0005 | 0.0002 | 0.0007 | 0.0004 | 0.0001 |
Golgi | 0.0023 | 0.0009 | 0.0007 | 0.0007 | 0.0007 | 0.0005 | 0.0089 | 0.0004 | 0.014 | 0.0093 | 0.0018 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0004 | 0.0001 |
Lipid Particles | 0.0045 | 0.001 | 0.0014 | 0.0011 | 0.0016 | 0.0013 | 0.0182 | 0.0008 | 0.0103 | 0.0017 | 0.0051 | 0.0015 | 0.0013 | 0.0096 | 0.0009 | 0.002 | 0.001 | 0.0002 |
Mitochondria | 0.0014 | 0.0012 | 0.0004 | 0.0006 | 0.0007 | 0.0003 | 0.0061 | 0.0002 | 0.0082 | 0.0042 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
None | 0.3891 | 0.2316 | 0.2965 | 0.3952 | 0.2866 | 0.3377 | 0.257 | 0.274 | 0.2379 | 0.2659 | 0.1699 | 0.2561 | 0.2979 | 0.2257 | 0.3107 | 0.3039 | 0.198 | 0.3143 |
Nuclear Periphery | 0.0012 | 0.0004 | 0.0004 | 0.0009 | 0.001 | 0.0003 | 0.028 | 0.0004 | 0.0008 | 0.0005 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0002 |
Nucleolus | 0.0019 | 0.0009 | 0.0008 | 0.0013 | 0.0004 | 0.0006 | 0.0035 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Nucleus | 0.0037 | 0.0025 | 0.0018 | 0.0024 | 0.0006 | 0.001 | 0.0109 | 0.0008 | 0.0009 | 0.0008 | 0.0005 | 0.0005 | 0.0015 | 0.0011 | 0.0004 | 0.0007 | 0.0004 | 0.0005 |
Peroxisomes | 0.0014 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0222 | 0.0001 | 0.0089 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0037 | 0.0056 | 0.002 | 0.0025 | 0.0007 | 0.0015 | 0.0098 | 0.002 | 0.0013 | 0.0003 | 0.002 | 0.0055 | 0.0004 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 |
Vacuole | 0.0049 | 0.0026 | 0.0049 | 0.0077 | 0.0061 | 0.0028 | 0.0094 | 0.0015 | 0.0024 | 0.005 | 0.0021 | 0.0014 | 0.003 | 0.0054 | 0.0017 | 0.0037 | 0.01 | 0.0008 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0041 | 0.0001 | 0.0022 | 0 | 0.0005 | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.7876 | 9.55 | 5.0618 | 10.1462 | 15.8297 | 10.6771 | 11.3803 | 10.2412 | 13.1216 | 18.4994 |
Translational Efficiency | 2.0568 | 1.6269 | 1.2149 | 1.2027 | 1.3675 | 2.9999 | 1.2576 | 1.3484 | 1.05 | 1.059 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2020 | 1100 | 1090 | 1817 | 319 | 2464 | 1514 | 1587 | 2339 | 3564 | 2604 | 3404 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 706.34 | 721.08 | 876.01 | 900.34 | 674.75 | 770.99 | 813.00 | 959.91 | 702.03 | 755.59 | 839.38 | 928.11 |
Standard Deviation | 79.66 | 106.36 | 125.89 | 162.52 | 73.02 | 95.06 | 115.37 | 185.18 | 79.53 | 101.34 | 123.85 | 175.98 |
Intensity Change Log 2 | — | 0.029797 | 0.310585 | 0.350107 | — | 0.192359 | 0.268902 | 0.508546 | — | 0.111508 | 0.290371 | 0.429689 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006111 | 0.005608 | 0.010897 | 0.047432 | 0.000440 | 0.004131 | 0.008927 | 0.036765 | 0.005338 | 0.004587 | 0.009752 | 0.042459 |
Bud Neck | 0.069465 | 0.044858 | 0.051342 | 0.046546 | 0.028599 | 0.077098 | 0.027818 | 0.053144 | 0.063891 | 0.067147 | 0.037665 | 0.049622 |
Bud Site | 0.023040 | 0.046238 | 0.118066* | 0.170615* | 0.005565 | 0.048520 | 0.054519 | 0.164610* | 0.020657 | 0.047816 | 0.081119 | 0.167816* |
Cell Periphery | 0.006057 | 0.002704 | 0.032200 | 0.100080* | 0.002723 | 0.003376 | 0.029354 | 0.247512* | 0.005603 | 0.003169 | 0.030545 | 0.168816* |
Cytoplasm | 0.507200* | 0.440011* | 0.423920* | 0.372144* | 0.521300* | 0.516045* | 0.429384* | 0.303474* | 0.509123* | 0.492577* | 0.427097* | 0.340129* |
Cytoplasmic Foci | 0.135898 | 0.252438* | 0.047280 | 0.010598 | 0.211772* | 0.123646 | 0.041974 | 0.009329 | 0.146246 | 0.163397 | 0.044195 | 0.010007 |
Eisosomes | 0.020941 | 0.004480 | 0.007691 | 0.024090 | 0.004532 | 0.006649 | 0.008702 | 0.034344 | 0.018703 | 0.005980 | 0.008279 | 0.028871 |
Endoplasmic Reticulum | 0.002726 | 0.002765 | 0.009925 | 0.005261 | 0.001219 | 0.001700 | 0.010406 | 0.006735 | 0.002521 | 0.002028 | 0.010204 | 0.005948 |
Endosome | 0.008446 | 0.024349 | 0.002248 | 0.002723 | 0.007882 | 0.009960 | 0.001769 | 0.001661 | 0.008369 | 0.014401 | 0.001970 | 0.002228 |
Golgi | 0.013479 | 0.018104 | 0.000997 | 0.002289 | 0.003691 | 0.006666 | 0.000451 | 0.001743 | 0.012144 | 0.010196 | 0.000679 | 0.002035 |
Lipid Particles | 0.016658 | 0.013320 | 0.003591 | 0.002073 | 0.011322 | 0.007743 | 0.002875 | 0.001930 | 0.015930 | 0.009465 | 0.003175 | 0.002006 |
Mitochondria | 0.007499 | 0.007380 | 0.000231 | 0.001496 | 0.001446 | 0.005687 | 0.000230 | 0.001057 | 0.006674 | 0.006209 | 0.000230 | 0.001292 |
Mitotic Spindle | 0.000670 | 0.001809 | 0.000169 | 0.005021 | 0.000163 | 0.002221 | 0.000501 | 0.001048 | 0.000601 | 0.002094 | 0.000362 | 0.003169 |
None | 0.001766 | 0.002470 | 0.001282 | 0.000821 | 0.000702 | 0.003724 | 0.002239 | 0.000758 | 0.001621 | 0.003337 | 0.001838 | 0.000792 |
Nuclear Periphery | 0.000132 | 0.000164 | 0.000184 | 0.000144 | 0.000246 | 0.000130 | 0.000309 | 0.000104 | 0.000148 | 0.000140 | 0.000257 | 0.000125 |
Nuclear Periphery Foci | 0.000316 | 0.000513 | 0.002548 | 0.000637 | 0.000378 | 0.000266 | 0.001829 | 0.000637 | 0.000324 | 0.000342 | 0.002130 | 0.000637 |
Nucleolus | 0.000427 | 0.000571 | 0.000133 | 0.000086 | 0.000358 | 0.000851 | 0.000126 | 0.000046 | 0.000418 | 0.000764 | 0.000129 | 0.000067 |
Nucleus | 0.124433 | 0.051969 | 0.178053* | 0.072185 | 0.147956* | 0.108940 | 0.270427* | 0.054138 | 0.127641 | 0.091357 | 0.231760* | 0.063772 |
Peroxisomes | 0.012425 | 0.016328 | 0.000863 | 0.001160 | 0.004073 | 0.009899 | 0.000685 | 0.000966 | 0.011286 | 0.011883 | 0.000760 | 0.001070 |
Vacuole | 0.041625 | 0.061921 | 0.108292 | 0.133092 | 0.045177 | 0.061967 | 0.107409 | 0.079034 | 0.042109 | 0.061953 | 0.107779 | 0.107889 |
Vacuole Periphery | 0.000687 | 0.002002 | 0.000089 | 0.001505 | 0.000455 | 0.000782 | 0.000064 | 0.000966 | 0.000655 | 0.001159 | 0.000075 | 0.001253 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.49 | -3.77 | -15.69 | -15.78 | -13.33 | -9.95 | -8.96 | -19.81 | -17.43 | -13.69 | 1.07 | -4.52 | -21.68 | -23.38 | -18.93 |
Bud Neck | 6.69 | 4.45 | 7.85 | 0.52 | 1.54 | -9.32 | 0.40 | -4.64 | 6.99 | -8.58 | -1.01 | 9.11 | 5.76 | 7.82 | -4.65 |
Bud Site | -6.86 | -15.18 | -24.94 | -18.68 | -5.62 | -17.01 | -14.23 | -28.37* | -19.25 | -17.53 | -11.98 | -17.15 | -35.07 | -26.80 | -16.76 |
Cell Periphery | 5.67 | -9.71 | -19.33 | -20.18 | -11.90 | -1.33 | -10.30 | -31.43* | -31.37* | -26.95* | 5.02 | -13.05 | -34.98 | -35.66* | -27.83* |
Cytoplasm | 7.57 | 10.22 | 17.35 | 7.00 | 4.94 | 0.38 | 7.83 | 16.65 | 26.31 | 12.68 | 2.77 | 13.84 | 26.77 | 24.92 | 11.97 |
Cytoplasmic Foci | -16.77 | 21.41 | 35.83 | 39.63 | 15.33 | 9.12 | 18.04 | 21.83 | 37.33 | 15.75 | -3.92 | 28.57 | 41.21 | 51.35 | 21.96 |
Eisosomes | 12.47 | 9.79 | -0.58 | -12.39 | -9.90 | -2.56 | -3.50 | -15.35 | -14.93 | -13.39 | 11.71 | 9.42 | -5.82 | -19.03 | -16.42 |
Endoplasmic Reticulum | -0.08 | -8.65 | -4.98 | -4.91 | 5.40 | -2.01 | -11.11 | -9.00 | -8.30 | 3.69 | 1.83 | -12.44 | -8.05 | -10.29 | 6.45 |
Endosome | -9.16 | 11.91 | 8.87 | 12.67 | -0.54 | -1.41 | 4.38 | 4.36 | 13.13 | 0.43 | -7.63 | 13.33 | 11.33 | 17.55 | -0.39 |
Golgi | -3.08 | 19.64 | 13.24 | 10.92 | -1.86 | -3.51 | 4.18 | 2.08 | 7.61 | -2.35 | 2.66 | 20.54 | 14.90 | 13.00 | -3.08 |
Lipid Particles | 2.70 | 14.37 | 15.39 | 11.98 | 4.00 | 2.91 | 7.55 | 8.40 | 12.33 | 4.42 | 7.26 | 16.13 | 17.31 | 16.47 | 5.32 |
Mitochondria | 0.12 | 10.27 | 8.04 | 5.96 | -4.08 | -7.04 | 3.40 | 1.04 | 9.17 | -5.97 | 0.62 | 10.48 | 8.57 | 10.41 | -6.09 |
Mitotic Spindle | -1.26 | 1.59 | -3.49 | -1.96 | -4.05 | -3.45 | -1.17 | -1.85 | 1.60 | -1.04 | -2.70 | 0.79 | -3.49 | -1.14 | -4.05 |
None | -1.04 | 1.50 | 2.52 | 2.30 | 0.99 | -5.16 | -2.46 | -0.18 | 4.72 | 2.20 | -3.62 | -0.30 | 3.12 | 5.18 | 2.36 |
Nuclear Periphery | -0.93 | -4.51 | -0.97 | 0.05 | 1.16 | 3.48 | -2.68 | 4.25 | 1.77 | 14.36 | 0.40 | -9.49 | 0.58 | 0.23 | 7.90 |
Nuclear Periphery Foci | -3.39 | -13.70 | -5.64 | -2.51 | 11.24 | 2.33 | -11.29 | -2.98 | -4.41 | 8.03 | -0.60 | -17.55 | -5.89 | -6.21 | 13.54 |
Nucleolus | -1.44 | 5.23 | 5.38 | 4.81 | 1.55 | -2.26 | 3.76 | 5.10 | 3.79 | 10.89 | -2.28 | 5.88 | 6.83 | 4.65 | 3.60 |
Nucleus | 18.78 | -8.41 | 11.73 | -6.76 | 16.12 | 4.62 | -12.66 | 10.84 | 13.70 | 33.28 | 10.20 | -20.61 | 17.58 | 8.56 | 35.32 |
Peroxisomes | -2.76 | 15.54 | 14.81 | 12.59 | -1.37 | -6.72 | 7.63 | 6.91 | 11.78 | -2.23 | -0.66 | 16.26 | 15.64 | 16.72 | -2.42 |
Vacuole | -7.15 | -16.13 | -23.75 | -17.85 | -6.28 | -4.83 | -15.63 | -9.13 | -6.18 | 6.36 | -10.44 | -24.67 | -25.30 | -18.37 | -1.74 |
Vacuole Periphery | -5.65 | 9.94 | -1.46 | 1.22 | -2.76 | -3.07 | 4.26 | -0.99 | -0.33 | -1.82 | -5.25 | 10.93 | -1.52 | -0.04 | -3.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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