Standard name | |
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Human Ortholog | |
Description | O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 41 | 38 | 161 | 203 | 201 | 352 | 278 | 164 | 267 | 254 | 352 | 296 | 39 | 85 | 35 | 31 | 45 | 30 | 3 | 1 | 7 | 7 | 13 | 13 |
Bud | 7 | 0 | 18 | 28 | 72 | 115 | 123 | 76 | 101 | 89 | 93 | 106 | 1 | 3 | 0 | 3 | 2 | 5 | 1 | 0 | 3 | 2 | 8 | 13 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 8 | 10 |
Bud Site | 0 | 0 | 1 | 2 | 3 | 13 | 8 | 2 | 4 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 262 | 81 | 373 | 351 | 309 | 385 | 305 | 205 | 318 | 304 | 428 | 393 | 196 | 291 | 233 | 262 | 252 | 200 | 322 | 128 | 468 | 279 | 237 | 291 |
Cytoplasm | 8 | 12 | 30 | 28 | 49 | 52 | 65 | 65 | 72 | 104 | 97 | 134 | 8 | 17 | 19 | 24 | 36 | 38 | 3 | 4 | 3 | 1 | 2 | 1 |
Endoplasmic Reticulum | 5 | 1 | 8 | 12 | 14 | 22 | 34 | 30 | 18 | 24 | 16 | 23 | 4 | 8 | 10 | 19 | 9 | 10 | 1 | 0 | 10 | 4 | 6 | 7 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Golgi | 1 | 5 | 1 | 16 | 12 | 2 | 6 | 13 | 1 | 2 | 4 | 0 | 0 | 1 | 2 | 2 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 45 | 42 | 140 | 213 | 205 | 422 | 429 | 201 | 296 | 224 | 349 | 333 | 5 | 17 | 3 | 50 | 29 | 18 | 1 | 21 | 6 | 14 | 16 | 24 |
Nucleus | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 4 | 3 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2 | 0 | 1 | 6 | 18 | 8 | 7 | 9 | 0 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 5 | 6 | 18 | 0 | 6 | 0 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 1 | 7 | 12 | 19 | 12 | 7 | 8 | 6 | 10 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 3 | 6 | 11 | 10 | 5 | 7 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 0 | 6 | 0 | 7 | 19 | 1 | 13 | 3 | 6 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 4 | 1 | 0 | 0 | 1 |
Unique Cell Count | 282 | 109 | 436 | 480 | 524 | 770 | 791 | 554 | 615 | 621 | 742 | 793 | 208 | 316 | 263 | 302 | 293 | 239 | 336 | 167 | 506 | 320 | 297 | 377 |
Labelled Cell Count | 369 | 180 | 736 | 865 | 889 | 1400 | 1305 | 822 | 1101 | 1042 | 1361 | 1328 | 253 | 422 | 302 | 392 | 384 | 305 | 336 | 167 | 506 | 320 | 297 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 22.4 | 23.1 | 16.8 | 16.2 | 15.3 | 13.4 | 12.2 | 13.7 | 12.4 | 12.1 | 12.1 | 11.7 | 21.0 | 20.2 | 21.3 | 21.3 | 21.1 | 21.4 | 26.8 | 32.3 | 36.8 |
Std Deviation (1e-4) | 3.9 | 4.8 | 4.5 | 3.3 | 4.2 | 3.8 | 3.3 | 4.0 | 4.1 | 3.7 | 3.5 | 4.1 | 3.3 | 3.7 | 4.3 | 5.3 | 7.1 | 7.0 | 6.6 | 10.4 | 11.9 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.14 | -0.33 | -0.46 | -0.3 | -0.44 | -0.48 | -0.48 | -0.52 | 0.32 | 0.27 | 0.34 | 0.34 | 0.33 | 0.34 | 0.67 | 0.94 | 1.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.7 | 0.5 | 3.0 | -0.6 | -2.4 | 2.1 | 1.3 | 3.5 | 0.1 | -4.7 | -2.9 | -6.7 | -8.1 | -6.3 | -6.7 |
Bud | 1.2 | 5.1 | 5.8 | 6.0 | 5.1 | 6.2 | 5.4 | 4.8 | 5.1 | -2.6 | -2.6 | -3.3 | -2.5 | -2.8 | -1.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -4.6 | -9.0 | -12.3 | -15.8 | -15.4 | -11.4 | -12.2 | -9.9 | -12.5 | 3.2 | 2.8 | 1.1 | 0.5 | 0.2 | -0.6 |
Cytoplasm | -0.7 | 1.4 | -0.1 | 0.8 | 2.6 | 2.6 | 4.7 | 3.3 | 4.9 | -1.5 | -0.8 | 0.2 | 0.5 | 2.5 | 3.7 |
Endoplasmic Reticulum | 0.7 | 0.9 | 1.1 | 2.3 | 2.9 | 1.1 | 1.9 | 0.4 | 1.1 | 0 | 0.7 | 1.6 | 3.2 | 1.1 | 1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.5 | 2.8 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.8 | 2.3 | 7.6 | 7.4 | 1.4 | 5.2 | 1.3 | 5.0 | 3.4 | -8.4 | -8.9 | -9.8 | -4.8 | -7.0 | -7.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.8 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.6 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.8494 | 20.5635 | 17.9233 | 16.1694 | 17.6059 | 17.8883 | 14.995 | 23.4994 | 18.6541 | 17.6661 | 18.6417 | 19.5701 | 12.5374 | 19.1715 | 16.9565 | 16.1496 | 14.5837 | 16.8963 |
Actin | 0.0147 | 0 | 0.0118 | 0.0042 | 0 | 0.0003 | 0.0361 | 0 | 0.0261 | 0.0116 | 0.029 | 0.0064 | 0.0144 | 0 | 0.0051 | 0.0014 | 0 | 0.0078 |
Bud | 0.0017 | 0 | 0.0004 | 0.0017 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0003 | 0.0017 | 0.0003 | 0.0009 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 |
Bud Neck | 0.0004 | 0.0001 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0025 | 0 | 0.0006 | 0.0004 | 0.0004 | 0.0007 | 0.0009 | 0.0002 | 0.005 | 0.0013 | 0 | 0.0003 |
Bud Periphery | 0.0106 | 0.0007 | 0.0038 | 0.0137 | 0.0034 | 0.0018 | 0.0066 | 0.0002 | 0.0046 | 0.0037 | 0.0089 | 0.0029 | 0.0102 | 0.0003 | 0.0013 | 0.0062 | 0.0005 | 0.0016 |
Bud Site | 0.0061 | 0.0012 | 0.0011 | 0.0016 | 0.0001 | 0.0001 | 0.0137 | 0.001 | 0.0042 | 0.0007 | 0.0012 | 0.0008 | 0.0045 | 0.0209 | 0.0032 | 0.0014 | 0 | 0.0007 |
Cell Periphery | 0.8548 | 0.9642 | 0.9545 | 0.8353 | 0.9655 | 0.9681 | 0.8483 | 0.9844 | 0.9126 | 0.9207 | 0.9266 | 0.9565 | 0.855 | 0.956 | 0.9407 | 0.9078 | 0.9883 | 0.967 |
Cytoplasm | 0.0086 | 0.003 | 0.0025 | 0.0022 | 0.0012 | 0.0013 | 0.009 | 0.0038 | 0.0045 | 0.0039 | 0.0017 | 0.0018 | 0.007 | 0.0068 | 0.0036 | 0.0055 | 0.0007 | 0.0015 |
Cytoplasmic Foci | 0.0031 | 0 | 0 | 0.0039 | 0 | 0.0012 | 0.0035 | 0 | 0.0043 | 0.0055 | 0.0001 | 0.0067 | 0.0126 | 0 | 0.0024 | 0.0049 | 0 | 0.0004 |
Eisosomes | 0.017 | 0.0016 | 0.002 | 0.0005 | 0.0023 | 0.0023 | 0.0065 | 0.0002 | 0.0013 | 0.0007 | 0.0123 | 0.0012 | 0.0046 | 0.0004 | 0.0016 | 0.0003 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0155 | 0.0143 | 0.0089 | 0.0474 | 0.0087 | 0.009 | 0.012 | 0.0023 | 0.0044 | 0.0083 | 0.0074 | 0.0023 | 0.0081 | 0.0038 | 0.0023 | 0.0149 | 0.0026 | 0.0076 |
Endosome | 0.0044 | 0 | 0 | 0.0215 | 0 | 0.0012 | 0.0077 | 0 | 0.0076 | 0.0021 | 0.0008 | 0.0025 | 0.0041 | 0 | 0.0044 | 0.0021 | 0 | 0.0001 |
Golgi | 0.003 | 0 | 0.0001 | 0.0099 | 0 | 0.0021 | 0.0039 | 0 | 0.0043 | 0.002 | 0.0008 | 0.0012 | 0.0043 | 0 | 0.0012 | 0.0004 | 0 | 0.0003 |
Lipid Particles | 0.0065 | 0.0001 | 0.0001 | 0.0056 | 0.0002 | 0.0004 | 0.0022 | 0 | 0.0011 | 0.0167 | 0.0002 | 0.0005 | 0.012 | 0.0004 | 0.0033 | 0.0016 | 0 | 0.0003 |
Mitochondria | 0.0236 | 0.0001 | 0.0027 | 0.0175 | 0.0048 | 0.0006 | 0.0162 | 0 | 0.0092 | 0.0065 | 0.0009 | 0.0078 | 0.01 | 0 | 0.0026 | 0.0011 | 0 | 0.0015 |
None | 0.0027 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0018 | 0 | 0.0011 | 0.0006 | 0.0002 | 0.0002 | 0.0092 | 0.0001 | 0.002 | 0.0046 | 0 | 0.0001 |
Nuclear Periphery | 0.002 | 0.0015 | 0.0008 | 0.002 | 0.0005 | 0.0006 | 0.0022 | 0.0006 | 0.0009 | 0.0009 | 0.0012 | 0.0003 | 0.0028 | 0.0007 | 0.0044 | 0.002 | 0.0002 | 0.0004 |
Nucleolus | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0015 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0032 | 0 | 0.0011 | 0.0012 | 0 | 0 |
Nucleus | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0026 | 0.0001 | 0.0013 | 0.0012 | 0 | 0.0001 |
Peroxisomes | 0.0024 | 0 | 0.0001 | 0.0143 | 0 | 0.0004 | 0.0021 | 0 | 0.0005 | 0.0024 | 0 | 0.0005 | 0.0099 | 0 | 0.0013 | 0.001 | 0 | 0.0004 |
Punctate Nuclear | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0048 | 0 | 0.0023 | 0.0216 | 0 | 0 |
Vacuole | 0.0208 | 0.0128 | 0.0106 | 0.0143 | 0.0123 | 0.0101 | 0.0212 | 0.0074 | 0.011 | 0.0121 | 0.0057 | 0.0072 | 0.0183 | 0.0102 | 0.0103 | 0.0184 | 0.0075 | 0.0092 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0002 | 0.0016 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0005 | 0.0007 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.0972 | 25.2917 | 20.709 | 30.2828 | 32.8876 | 21.4513 | 34.17 | 30.6508 | 22.8342 | 45.4362 |
Translational Efficiency | 1.5801 | 1.8096 | 1.5935 | 0.8272 | 1.4239 | 3.0465 | 1.3422 | 1.1146 | 1.5921 | 1.0902 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
182 | 1837 | 294 | 913 | 1443 | 1296 | 2335 | 1896 | 1625 | 3133 | 2629 | 2809 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1538.45 | 1672.29 | 1870.49 | 1966.83 | 1319.06 | 1656.35 | 1730.87 | 1929.66 | 1343.63 | 1665.70 | 1746.48 | 1941.74 |
Standard Deviation | 244.13 | 347.65 | 223.80 | 342.84 | 225.95 | 275.87 | 233.20 | 349.13 | 238.33 | 320.01 | 236.30 | 347.53 |
Intensity Change Log 2 | — | 0.120347 | 0.281939 | 0.354395 | — | 0.328497 | 0.391987 | 0.548836 | — | 0.220174 | 0.333783 | 0.447417 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000354 | 0.001981 | 0.004057 | 0.001959 | 0.001408 | 0.002138 | 0.000236 | 0.001173 | 0.001290 | 0.002046 | 0.000664 | 0.001429 |
Bud Neck | 0.000655 | 0.003714 | 0.006196 | 0.000706 | 0.001854 | 0.000879 | 0.000454 | 0.000406 | 0.001720 | 0.002541 | 0.001097 | 0.000503 |
Bud Site | 0.006456 | 0.043255 | 0.031086 | 0.010210 | 0.023146 | 0.010319 | 0.002788 | 0.008957 | 0.021277 | 0.029630 | 0.005953 | 0.009364 |
Cell Periphery | 0.974443* | 0.871225* | 0.931720* | 0.976478* | 0.940261* | 0.975268* | 0.993403* | 0.972210* | 0.944089* | 0.914263* | 0.986505* | 0.973597* |
Cytoplasm | 0.001752 | 0.043850 | 0.003689 | 0.002190 | 0.019119 | 0.000644 | 0.000491 | 0.008449 | 0.017174 | 0.025977 | 0.000849 | 0.006415 |
Cytoplasmic Foci | 0.000400 | 0.004156 | 0.000357 | 0.000064 | 0.001356 | 0.000351 | 0.000032 | 0.000331 | 0.001249 | 0.002582 | 0.000068 | 0.000244 |
Eisosomes | 0.000432 | 0.000553 | 0.002462 | 0.001036 | 0.000450 | 0.001010 | 0.000623 | 0.000283 | 0.000448 | 0.000742 | 0.000829 | 0.000527 |
Endoplasmic Reticulum | 0.005421 | 0.004598 | 0.005470 | 0.000955 | 0.004962 | 0.003567 | 0.000322 | 0.000596 | 0.005013 | 0.004171 | 0.000898 | 0.000712 |
Endosome | 0.000376 | 0.004586 | 0.000915 | 0.000192 | 0.000982 | 0.001282 | 0.000074 | 0.000457 | 0.000914 | 0.003220 | 0.000168 | 0.000371 |
Golgi | 0.000418 | 0.004278 | 0.002092 | 0.000294 | 0.001009 | 0.000673 | 0.000081 | 0.001174 | 0.000943 | 0.002787 | 0.000306 | 0.000888 |
Lipid Particles | 0.000529 | 0.005302 | 0.001755 | 0.000084 | 0.001173 | 0.000083 | 0.000003 | 0.000294 | 0.001101 | 0.003143 | 0.000199 | 0.000225 |
Mitochondria | 0.003714 | 0.001543 | 0.000162 | 0.000609 | 0.001139 | 0.000790 | 0.000176 | 0.001590 | 0.001428 | 0.001231 | 0.000174 | 0.001271 |
Mitotic Spindle | 0.000419 | 0.000306 | 0.000780 | 0.000221 | 0.000243 | 0.000180 | 0.000096 | 0.000186 | 0.000263 | 0.000254 | 0.000173 | 0.000198 |
None | 0.000012 | 0.000135 | 0.000021 | 0.000014 | 0.000049 | 0.000021 | 0.000006 | 0.000056 | 0.000045 | 0.000088 | 0.000007 | 0.000043 |
Nuclear Periphery | 0.000350 | 0.000148 | 0.000510 | 0.000159 | 0.000117 | 0.000115 | 0.000103 | 0.000171 | 0.000143 | 0.000134 | 0.000148 | 0.000167 |
Nuclear Periphery Foci | 0.000218 | 0.000295 | 0.000344 | 0.000065 | 0.000183 | 0.000072 | 0.000022 | 0.000056 | 0.000187 | 0.000203 | 0.000058 | 0.000059 |
Nucleolus | 0.000020 | 0.000048 | 0.000015 | 0.000022 | 0.000024 | 0.000024 | 0.000015 | 0.000021 | 0.000024 | 0.000038 | 0.000015 | 0.000021 |
Nucleus | 0.000314 | 0.000228 | 0.000212 | 0.000067 | 0.000109 | 0.000037 | 0.000022 | 0.000090 | 0.000132 | 0.000149 | 0.000043 | 0.000082 |
Peroxisomes | 0.000045 | 0.000797 | 0.000065 | 0.000012 | 0.000244 | 0.000049 | 0.000005 | 0.000133 | 0.000222 | 0.000487 | 0.000012 | 0.000094 |
Vacuole | 0.001809 | 0.005111 | 0.007244 | 0.002114 | 0.000861 | 0.000971 | 0.000655 | 0.001705 | 0.000967 | 0.003399 | 0.001392 | 0.001838 |
Vacuole Periphery | 0.001864 | 0.003892 | 0.000847 | 0.002553 | 0.001312 | 0.001527 | 0.000392 | 0.001662 | 0.001374 | 0.002914 | 0.000443 | 0.001952 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.05 | -3.77 | -1.77 | 0.02 | 1.59 | -0.93 | 4.08 | 0.51 | 1.18 | -2.58 | -1.76 | 2.25 | -0.31 | 1.20 | -1.93 |
Bud Neck | -7.63 | -7.00 | -0.18 | 9.20 | 7.25 | 3.95 | 6.74 | 7.19 | 2.96 | 0.54 | -3.14 | 3.00 | 6.69 | 10.18 | 4.87 |
Bud Site | -8.82 | -4.17 | -1.06 | 9.57 | 3.85 | 4.41 | 8.68 | 5.41 | 0.60 | -4.17 | -3.02 | 6.98 | 5.16 | 9.32 | -2.51 |
Cell Periphery | 9.22 | 3.68 | -0.20 | -14.97 | -5.85 | -6.18 | -11.62 | -6.08 | 0.70 | 7.63 | 5.21 | -9.89 | -6.32 | -13.19 | 5.43 |
Cytoplasm | -13.41 | -2.08 | -0.35 | 12.92 | 1.26 | 8.08 | 8.16 | 4.16 | -6.51 | -6.68 | -3.22 | 8.03 | 4.87 | 9.65 | -6.34 |
Cytoplasmic Foci | -5.29 | 0.18 | 1.60 | 6.04 | 2.75 | 3.82 | 5.99 | 3.86 | 0.10 | -2.05 | -2.97 | 5.96 | 4.54 | 5.63 | -1.77 |
Eisosomes | -1.21 | -7.32 | -3.00 | -2.56 | 4.45 | -3.89 | -2.58 | 3.65 | 5.21 | 5.97 | -3.94 | -5.58 | -1.11 | 2.41 | 3.61 |
Endoplasmic Reticulum | 0.25 | -0.01 | 1.37 | 5.34 | 6.46 | 1.26 | 7.15 | 6.69 | 3.31 | -3.03 | 1.00 | 6.01 | 6.20 | 6.72 | 1.16 |
Endosome | -4.56 | -2.39 | 1.10 | 4.82 | 4.32 | -0.72 | 5.44 | 2.28 | 1.93 | -2.44 | -3.99 | 4.96 | 2.94 | 5.01 | -1.87 |
Golgi | -5.94 | -5.43 | 0.84 | 6.26 | 6.44 | 1.33 | 5.66 | -0.39 | -1.12 | -2.70 | -4.52 | 4.24 | 0.17 | 4.04 | -2.11 |
Lipid Particles | -4.47 | -1.43 | 0.93 | 5.44 | 2.34 | 2.29 | 2.47 | 1.79 | -1.54 | -2.19 | -2.90 | 2.09 | 2.02 | 5.11 | -0.22 |
Mitochondria | 0.60 | 0.99 | 0.86 | 2.13 | -1.30 | 0.62 | 1.80 | -0.52 | -1.13 | -2.06 | 0.30 | 2.01 | 0.20 | -0.08 | -2.29 |
Mitotic Spindle | 0.55 | -1.74 | 0.97 | 2.67 | 11.27 | 3.46 | 10.23 | 1.83 | -0.22 | -3.22 | 0.32 | 3.35 | 1.98 | 2.36 | -1.14 |
None | -2.56 | -2.52 | -0.68 | 2.51 | 2.45 | 1.49 | 2.49 | -0.18 | -0.91 | -1.33 | -1.34 | 2.42 | 0.07 | 1.18 | -1.37 |
Nuclear Periphery | 0.80 | -0.62 | 0.75 | -0.78 | 10.00 | 0.14 | 1.52 | -4.94 | -4.87 | -7.37 | 0.29 | -0.18 | -0.80 | -3.83 | -2.06 |
Nuclear Periphery Foci | -0.58 | -0.97 | 1.19 | 5.17 | 8.80 | 2.73 | 5.20 | 3.96 | 0.58 | -3.82 | -0.39 | 4.15 | 3.98 | 5.31 | -0.10 |
Nucleolus | -3.75 | 0.71 | -0.39 | 5.33 | -2.98 | -0.08 | 7.26 | 1.68 | 0.87 | -4.19 | -4.50 | 6.51 | 1.32 | 5.23 | -5.32 |
Nucleus | 0.32 | 0.38 | 0.93 | 7.47 | 7.81 | 4.18 | 5.49 | 1.08 | -4.89 | -8.15 | -0.50 | 2.70 | 1.49 | 4.87 | -5.90 |
Peroxisomes | -7.24 | -0.54 | 1.00 | 7.99 | 3.57 | 3.57 | 4.90 | 1.32 | -1.16 | -1.89 | -3.63 | 4.83 | 2.02 | 5.28 | -1.79 |
Vacuole | -2.36 | -3.57 | -0.23 | 4.08 | 5.47 | -0.53 | 2.11 | -3.02 | -2.17 | -3.84 | -5.95 | -2.17 | -3.23 | 3.56 | -1.83 |
Vacuole Periphery | -1.73 | 0.97 | -0.47 | 1.12 | -1.61 | -0.47 | 6.60 | -1.08 | -0.26 | -4.22 | -3.84 | 5.45 | -1.35 | 1.76 | -3.79 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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