Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.06 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.96 0.92 – 0.68 0.66 0.62 0.62 0.61 0.99 0.98 0.97 0.99 0.85 1.0 0.98 0.96 0.91 0.94 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0.06 – 0.23 0.21 0.19 0.2 0.21 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0.05 0.11 0.15 0.24 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 0 7 – 16 17 44 16 15 0 0 0 0 1 0 0 0 0 0 0 0
Bud 1 1 1 1 0 0 – 2 4 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 2 0 9 15 – 19 24 41 20 20 0 1 6 0 0 0 0 0 0 0 0 0
Cytoplasm 604 306 194 195 376 409 – 361 285 326 209 209 177 278 294 84 52 18 587 286 181 100 234 235
Endoplasmic Reticulum 5 2 3 1 16 28 – 124 90 99 67 71 1 7 11 3 1 1 1 2 6 0 4 4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 0 2 – 18 10 32 8 9 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1 1 0 9 – 26 50 80 81 81 0 0 0 0 1 0 0 0 0 0 0 0
Nucleus 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1
Nuclear Periphery 0 0 0 0 0 0 – 0 3 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 1 0 1 – 14 16 12 2 3 1 0 0 0 6 0 4 1 1 1 4 4
Unique Cell Count 612 310 201 200 392 446 533 435 527 336 340 178 283 304 85 61 18 601 298 199 107 250 253
Labelled Cell Count 617 312 204 201 401 471 580 499 634 404 409 179 286 311 87 62 19 601 298 199 107 250 253


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 85.6 99.1 42.8 54.2 49.7 33.4 – 24.7 19.4 19.4 16.1 16.1 78.1 85.4 83.0 65.4 46.3 66.2 87.1 88.3 89.3
Std Deviation (1e-4) 19.9 21.7 12.1 16.4 10.6 8.2 – 6.1 5.7 5.1 4.3 4.3 20.3 20.6 19.0 21.3 23.0 12.8 21.3 25.6 27.5
Intensity Change (Log2) – – – 0.34 0.21 -0.36 – -0.8 -1.14 -1.14 -1.42 -1.42 0.87 1.0 0.95 0.61 0.11 0.63 1.02 1.04 1.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 2.4 3.9 2.7 2.6 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.7 – 1.9 2.7 3.5 2.8 2.8 0 0 0 0 0 0
Cytoplasm 0.6 -0.4 -2.3 – -8.1 -8.4 -9.3 -8.9 -9.0 2.0 1.2 0.1 1.1 -3.2 0.8
Endoplasmic Reticulum 0 1.7 2.6 – 7.0 6.4 6.0 6.1 6.3 0 0 1.4 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 3.6 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 2.8 4.7 5.6 7.4 7.3 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 2.3 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 55.2756 71.556 64.0134 58.9945 66.6932 70.4034 63.1097 84.6163 77.1092 72.5106 71.404 71.2961 61.9706 85.3581 74.6787 79.3899 63.6824 80.1177
Actin 0.0073 0.0006 0.0008 0.012 0.0002 0.0199 0.0077 0.0002 0.0008 0.0016 0.0034 0.0006 0.0165 0.0003 0.0187 0.0003 0.0597 0.0042
Bud 0.0013 0.0005 0.0014 0.0014 0.0003 0.0007 0.0013 0.0002 0.0015 0.0013 0.0005 0.0005 0.0021 0.0004 0.0009 0.0008 0.0005 0.0019
Bud Neck 0.0008 0.0004 0.0007 0.0008 0.0006 0.0015 0.0006 0.0003 0.0004 0.0008 0.0009 0.0016 0.0011 0.0002 0.0005 0.0006 0.0007 0.0032
Bud Periphery 0.0018 0.0003 0.0011 0.001 0.0001 0.0005 0.0017 0.0001 0.0011 0.0009 0.0003 0.0003 0.0023 0.0001 0.0005 0.0002 0.0004 0.0021
Bud Site 0.0012 0.0004 0.0006 0.0003 0 0.0001 0.0015 0.0002 0.0009 0.0006 0.0005 0.0002 0.0055 0.0003 0.0003 0.0001 0.0014 0.0004
Cell Periphery 0.0084 0.0057 0.0037 0.0018 0.0036 0.0018 0.0116 0.004 0.0019 0.0022 0.0014 0.0009 0.0061 0.001 0.0037 0.0009 0.0001 0.0055
Cytoplasm 0.7525 0.9277 0.8892 0.6629 0.8988 0.8431 0.7557 0.9595 0.9072 0.7593 0.8353 0.829 0.742 0.9747 0.8649 0.7717 0.8901 0.8139
Cytoplasmic Foci 0.0053 0.0015 0.0033 0.0039 0.0034 0.0038 0.0099 0.0014 0.0035 0.0066 0.0097 0.0205 0.0148 0.0017 0.0052 0.0039 0.0055 0.0047
Eisosomes 0.0002 0 0 0.0003 0 0.0007 0.0002 0 0 0.0001 0 0.0001 0.0004 0 0.0003 0 0.0003 0
Endoplasmic Reticulum 0.0566 0.0145 0.0128 0.0227 0.017 0.0481 0.0309 0.0131 0.018 0.0288 0.0293 0.0481 0.0556 0.0058 0.0058 0.0176 0.0037 0.0717
Endosome 0.0178 0.0033 0.0098 0.0264 0.0077 0.0176 0.0333 0.0024 0.0119 0.0287 0.0336 0.0139 0.0201 0.0025 0.0086 0.0139 0.0109 0.0176
Golgi 0.0031 0.0017 0.0017 0.0116 0.0003 0.0039 0.0055 0.0003 0.0022 0.0058 0.0301 0.0046 0.0067 0.0005 0.0022 0.0007 0.0033 0.0031
Lipid Particles 0.0053 0.0015 0.0007 0.0006 0.0007 0.0019 0.0068 0.0005 0.0004 0.0068 0.0052 0.0049 0.0088 0.0001 0.001 0.0001 0.0026 0.0008
Mitochondria 0.01 0.0007 0.0149 0.0107 0.0001 0.0019 0.0127 0.0001 0.0063 0.0052 0.0076 0.0037 0.0135 0.0001 0.0129 0.0003 0.0009 0.0024
None 0.0056 0.0002 0.0006 0.0005 0.0003 0.022 0.0027 0.0001 0.0006 0.001 0.0009 0.0052 0.0058 0.0001 0.0005 0.0002 0.0007 0.0005
Nuclear Periphery 0.0248 0.0054 0.0035 0.0142 0.0044 0.0084 0.0172 0.0012 0.0033 0.0054 0.0068 0.0101 0.0197 0.0007 0.0034 0.0165 0.0024 0.0084
Nucleolus 0.0019 0.0001 0.0031 0.0001 0 0.0004 0.0019 0 0.0002 0.0002 0.0002 0.0165 0.0084 0 0.0003 0.0001 0.0001 0.0001
Nucleus 0.0056 0.0016 0.0026 0.0028 0.0013 0.0021 0.0068 0.0008 0.0017 0.0024 0.0019 0.0047 0.0075 0.0007 0.0021 0.0028 0.0014 0.0021
Peroxisomes 0.0014 0 0.0001 0.0001 0 0.0001 0.0015 0 0.0001 0.0003 0.0016 0.0003 0.0015 0 0.0036 0.0001 0.0025 0.0001
Punctate Nuclear 0.0015 0 0.0001 0.0001 0 0.0008 0.0013 0 0.0001 0.0002 0.0001 0.002 0.0042 0 0.0014 0.0001 0.0006 0.0001
Vacuole 0.068 0.0308 0.0408 0.1475 0.0528 0.0143 0.0728 0.0144 0.0314 0.1147 0.022 0.0201 0.0452 0.0099 0.0518 0.1298 0.0098 0.0454
Vacuole Periphery 0.0193 0.0029 0.0083 0.0784 0.0082 0.0064 0.0163 0.001 0.0061 0.0273 0.0087 0.0121 0.0124 0.0009 0.0114 0.0393 0.0025 0.0117

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1098.343 1155.2969 1235.5578 1153.347 1275.301 994.875 940.1379 936.6132 1075.0526 982.5518
Translational Efficiency 1.7467 1.807 1.7952 2.1256 1.8944 1.9497 2.0108 2.4383 2.0771 1.9978

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
138 777 1407 1198 1202 1674 2284 1198 1340 2451 3691 2396

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3646.76 5683.87 7133.04 7148.43 3938.85 5115.49 8015.32 6682.70 3908.77 5295.67 7679.00 6915.56
Standard Deviation 925.78 1523.00 1518.01 2459.40 1022.66 1243.57 1734.85 2271.76 1017.00 1364.36 1710.10 2378.86
Intensity Change Log 2 — 0.640258 0.967902 0.971011 — 0.377098 1.024986 0.762657 — 0.509609 0.997825 0.866582

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000093 0.000094 0.000111 0.000382 0.000070 0.000061 0.000086 0.000773 0.000073 0.000071 0.000095 0.000577
Bud Neck 0.001006 0.000541 0.002285 0.001783 0.000357 0.000466 0.000394 0.001645 0.000424 0.000490 0.001115 0.001714
Bud Site 0.007673 0.004532 0.003739 0.013236 0.002922 0.003069 0.002788 0.015168 0.003412 0.003533 0.003150 0.014202
Cell Periphery 0.031455 0.015722 0.014611 0.046688 0.016484 0.006605 0.025238 0.039430 0.018026 0.009495 0.021187 0.043059
Cytoplasm 0.433781* 0.739858* 0.592139* 0.525813* 0.656273* 0.756240* 0.769994* 0.583658* 0.633360* 0.751047* 0.702196* 0.554735*
Cytoplasmic Foci 0.030568 0.008967 0.015837 0.008943 0.008597 0.010912 0.003962 0.011014 0.010859 0.010295 0.008489 0.009979
Eisosomes 0.000002 0.000002 0.000003 0.000007 0.000001 0.000001 0.000002 0.000007 0.000001 0.000001 0.000002 0.000007
Endoplasmic Reticulum 0.001522 0.000636 0.000655 0.000709 0.001070 0.000657 0.000545 0.000441 0.001116 0.000650 0.000587 0.000575
Endosome 0.003753 0.000650 0.000931 0.001048 0.000923 0.000793 0.000398 0.002067 0.001214 0.000747 0.000601 0.001558
Golgi 0.176601* 0.061273 0.057969 0.034461 0.089506 0.060971 0.017867 0.032182 0.098475 0.061067 0.033154 0.033322
Lipid Particles 0.031563 0.012912 0.013257 0.029035 0.014503 0.006911 0.015763 0.025514 0.016260 0.008814 0.014808 0.027275
Mitochondria 0.000074 0.000230 0.000216 0.000967 0.000128 0.000172 0.000391 0.000467 0.000122 0.000190 0.000324 0.000717
Mitotic Spindle 0.002535 0.001426 0.003274 0.001287 0.000864 0.001802 0.000535 0.001242 0.001036 0.001683 0.001579 0.001265
None 0.001049 0.003572 0.001745 0.002442 0.001979 0.002751 0.002479 0.000953 0.001883 0.003011 0.002199 0.001698
Nuclear Periphery 0.000658 0.000747 0.002155 0.000705 0.000531 0.000612 0.000376 0.000409 0.000544 0.000655 0.001054 0.000557
Nuclear Periphery Foci 0.018437 0.014568 0.019295 0.007573 0.015773 0.010100 0.005188 0.005737 0.016048 0.011517 0.010566 0.006655
Nucleolus 0.000082 0.000087 0.000126 0.000224 0.000064 0.000070 0.000070 0.000248 0.000065 0.000075 0.000091 0.000236
Nucleus 0.003421 0.009042 0.018438 0.011748 0.005193 0.007603 0.006116 0.008109 0.005011 0.008060 0.010813 0.009929
Peroxisomes 0.000059 0.000019 0.000015 0.000090 0.000021 0.000014 0.000073 0.000204 0.000025 0.000015 0.000051 0.000147
Vacuole 0.110716 0.062954 0.160941 0.246875* 0.080922 0.064376 0.116121 0.224503* 0.083991 0.063925 0.133206 0.235689*
Vacuole Periphery 0.144952* 0.062167 0.092259 0.065985 0.103818* 0.065813 0.031613 0.046228 0.108054* 0.064658 0.054731 0.056106

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.28 -3.09 -4.22 -4.17 -3.71 0.55 -1.60 -2.22 -2.24 -2.15 -0.15 -3.41 -3.26 -3.25 -3.05
Bud Neck 1.98 -6.00 -3.94 -6.95 2.70 -1.83 -1.26 -6.89 -5.65 -6.63 -1.54 -8.35 -10.12 -8.84 -3.02
Bud Site 1.50 2.31 -3.26 -5.28 -6.65 -0.66 0.16 -8.66 -8.39 -9.04 -0.72 0.20 -10.48 -10.05 -11.20
Cell Periphery 3.88 4.30 -3.11 -11.50 -13.11 9.17 -5.74 -9.80 -15.67 -5.32 7.76 -2.67 -13.31 -20.52 -11.92
Cytoplasm -9.21 -4.32 -2.70 14.02 3.68 -8.04 -9.42 5.18 13.64 15.24 -10.24 -5.39 6.73 20.56 15.26
Cytoplasmic Foci 4.43 2.50 4.20 -0.92 5.23 -3.20 7.56 -2.58 -0.30 -6.90 0.38 1.55 0.03 -0.36 -1.57
Eisosomes 2.10 -3.41 -4.66 -5.20 -3.76 2.59 -2.29 -3.49 -3.72 -3.08 2.12 -4.88 -5.84 -6.14 -4.81
Endoplasmic Reticulum 2.52 2.55 2.45 -0.39 -0.45 1.95 3.61 4.53 1.37 1.90 2.70 3.93 4.19 0.76 0.32
Endosome 3.51 3.13 3.06 -2.82 -0.33 1.14 4.34 -1.83 -2.01 -2.72 3.69 4.50 -0.96 -2.50 -2.94
Golgi 7.88 8.37 10.06 7.46 9.20 6.64 19.57 14.85 10.07 -7.02 9.11 17.71 17.61 12.55 0.48
Lipid Particles 3.78 3.68 0.69 -6.69 -7.85 5.82 -1.07 -5.46 -10.91 -5.13 5.61 0.73 -6.47 -13.65 -8.93
Mitochondria -3.23 -7.67 -2.32 -1.88 -1.88 -2.15 -2.21 -1.97 -1.68 -0.39 -3.15 -2.85 -2.82 -2.46 -1.72
Mitotic Spindle 2.21 -2.03 1.84 -0.29 3.91 -3.15 1.78 -1.49 1.42 -2.33 -2.96 -3.37 -1.81 1.06 1.14
None -4.73 -3.48 -2.19 1.39 -1.13 -1.17 -0.86 5.33 2.74 2.62 -2.34 -0.87 0.50 2.30 1.04
Nuclear Periphery -0.67 -9.68 -0.49 0.36 11.42 -0.37 3.40 2.79 0.86 -0.79 -0.70 -9.17 -0.55 0.57 9.44
Nuclear Periphery Foci 1.29 -0.50 3.96 5.26 12.29 5.61 12.15 11.47 6.91 -1.06 4.58 5.71 11.46 8.08 8.98
Nucleolus -0.20 -3.05 -3.90 -3.72 -2.68 -1.27 -1.42 -2.48 -2.32 -2.39 -1.49 -6.51 -4.25 -3.87 -3.49
Nucleus -5.03 -12.25 -8.65 -4.22 4.02 -3.31 -1.90 -4.83 -1.58 -3.67 -4.56 -9.50 -8.61 -4.53 0.08
Peroxisomes 2.36 2.60 -1.30 -5.82 -6.38 1.82 -3.91 -3.74 -3.91 -2.61 2.36 -2.92 -4.77 -5.22 -3.63
Vacuole 3.57 -4.49 -9.61 -21.93 -8.39 3.12 -6.93 -17.81 -20.50 -13.08 4.29 -11.22 -24.44 -30.33 -16.23
Vacuole Periphery 4.88 3.31 4.90 -0.13 5.83 7.02 15.25 11.93 5.71 -5.05 8.63 11.59 11.36 3.52 -0.00
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps25b

Rps25b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps25b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available