Standard name
Human Ortholog
Description Protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.93 0.92 0.84 – 0.82 0.81 0.85 0.66 0.76 0.99 0.98 0.99 0.86 0.87 0.87 0.87 0.87 0.68 0.8 0.79 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.1 0.28 – 0.24 0.34 0.22 0.46 0.4 0 0 0 0 0 0.05 0.05 0.05 0.16 0.08 0.08 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 1 0 – 1 0 0 0 0 0 0 1 6 4 3 2 1 1 0 8 8
Bud 0 1 1 2 6 7 – 6 15 9 17 11 0 3 1 0 1 2 0 0 0 1 14 20
Bud Neck 2 4 0 0 9 10 – 20 10 12 11 4 0 1 2 0 1 0 1 0 0 1 5 5
Bud Site 0 0 0 0 0 2 – 0 0 1 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 2 2 0 1 – 2 1 1 0 5 1 4 4 10 7 10 0 3 2 0 4 4
Cytoplasm 283 254 88 190 279 340 – 506 353 325 216 395 361 738 883 126 116 129 255 236 51 82 401 540
Endoplasmic Reticulum 3 3 1 1 1 1 – 1 1 1 0 0 1 5 1 10 7 6 0 1 0 0 1 1
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 2 0 1 2 1 1 1 3 0 0 2 7
Golgi 1 0 0 0 0 1 – 0 0 0 0 0 0 2 0 1 0 0 1 2 0 0 1 3
Mitochondria 8 6 3 17 31 113 – 146 147 85 152 210 2 7 4 2 5 8 14 13 11 8 38 29
Nucleus 1 0 0 0 0 4 – 6 3 0 5 9 0 2 2 0 4 3 0 0 0 1 0 4
Nuclear Periphery 0 0 0 1 0 0 – 2 1 1 3 1 0 2 3 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 – 0 1 1 4 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 3 3 – 16 9 10 15 18 0 0 0 0 1 1 3 1 0 0 10 14
Vac/Vac Membrane 0 1 0 0 1 2 – 4 3 4 6 4 1 1 3 6 4 5 4 2 0 3 9 13
Unique Cell Count 292 262 91 204 304 403 614 437 382 328 522 366 751 894 146 134 149 293 273 76 103 509 667
Labelled Cell Count 303 272 95 214 331 484 710 545 450 431 666 368 765 905 163 151 168 293 273 76 103 509 667


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.9 3.3 3.0 3.7 2.8 – 2.9 2.5 2.9 2.7 2.6 4.2 4.4 4.2 7.1 7.3 6.9 3.9 4.2 4.4
Std Deviation (1e-4) 0.5 1.0 0.7 1.2 1.6 0.9 – 1.0 1.2 1.5 1.3 1.2 1.1 0.9 0.9 1.7 1.5 1.8 1.4 1.4 1.5
Intensity Change (Log2) – – – -0.12 0.17 -0.25 – -0.17 -0.39 -0.19 -0.25 -0.31 0.37 0.41 0.36 1.13 1.17 1.08 0.26 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4383 -0.1492 -0.4056 -0.7744 1.2489 -0.2643 -0.7937 -1.0018 -1.1332 -0.6428 -0.2659 -1.0327 0.7617 0.2593 0.2156 1.2985 0.3471 0.4588
Actin 0.0231 0.0004 0.003 0.0649 0.0154 0.0093 0.0103 0.0019 0.0078 0.0014 0.0081 0.0028 0.1178 0.0002 0.0056 0.0711 0 0.0077
Bud 0.002 0.0024 0.0006 0.0003 0.0034 0.0001 0.0029 0.0003 0.0008 0.0006 0.0002 0.0001 0.0006 0 0.0005 0.0006 0 0.0002
Bud Neck 0.009 0.0006 0.0004 0.0019 0.0039 0.0002 0.0022 0.0001 0.0003 0.0034 0.0001 0.0001 0.0177 0 0.0064 0.0001 0 0.0002
Bud Periphery 0.0063 0.0023 0.0007 0.0005 0.0067 0.0002 0.0101 0.0005 0.0034 0.001 0.0004 0.0002 0.0008 0 0.0007 0.0007 0 0.0006
Bud Site 0.0031 0.0085 0.0027 0.0007 0.0328 0.0001 0.0058 0.0049 0.0019 0.0154 0.0002 0.0001 0.0074 0.0002 0.0052 0.0032 0 0.0003
Cell Periphery 0.0005 0.0003 0.0001 0.0001 0.0013 0 0.0012 0.0007 0.0001 0.0012 0 0 0.0007 0.0001 0.0002 0 0 0.0001
Cytoplasm 0.0308 0.028 0.0412 0.0525 0.0387 0.0836 0.0218 0.0491 0.0383 0.0204 0.0052 0.0889 0.0551 0.0281 0.039 0.0581 0.0125 0.0726
Cytoplasmic Foci 0.0289 0.0052 0.0189 0.0078 0.0142 0.0158 0.0379 0.0043 0.0079 0.0256 0.0063 0.0048 0.0343 0.0023 0.0044 0.0046 0.001 0.0018
Eisosomes 0.0004 0.0002 0.0003 0.0005 0.0003 0.0002 0.0002 0.0005 0.0003 0.0001 0.0001 0.0002 0.0012 0.0004 0.0001 0.0003 0.0001 0.0004
Endoplasmic Reticulum 0.0053 0.0004 0.0007 0.0011 0.0035 0.0012 0.0076 0.001 0.0008 0.0005 0.0018 0.0004 0.0041 0.0004 0.0003 0.0002 0.0001 0.0018
Endosome 0.023 0.0003 0.0028 0.004 0.0243 0.0029 0.0405 0.0011 0.0065 0.017 0.0312 0.0035 0.0147 0.0003 0.0014 0.0004 0 0.0099
Golgi 0.0043 0.0001 0.0014 0.0046 0.008 0.0016 0.0144 0.0004 0.0042 0.0034 0.0437 0.009 0.0094 0.0001 0.001 0.0021 0 0.0025
Lipid Particles 0.0064 0.0003 0.0028 0.0051 0.0057 0.0044 0.0426 0.0016 0.0049 0.0283 0.0284 0.0014 0.0165 0.0008 0.0022 0.001 0.0001 0.0043
Mitochondria 0.0058 0.0003 0.0017 0.0009 0.0033 0.011 0.0101 0.0048 0.0135 0.0014 0.0428 0.026 0.0023 0.0002 0.0003 0.0006 0 0.0069
None 0.7705 0.9422 0.9126 0.8493 0.7241 0.8576 0.7434 0.9265 0.9049 0.8177 0.722 0.8602 0.6654 0.9657 0.9258 0.8486 0.9859 0.8785
Nuclear Periphery 0.0277 0.0003 0.0011 0.0008 0.0233 0.0028 0.0085 0.0004 0.0005 0.0007 0.0593 0.0003 0.0053 0.0002 0.0002 0.0001 0 0.006
Nucleolus 0.0026 0.0012 0.0004 0.0002 0.0044 0.0005 0.0024 0.0001 0.0001 0.0108 0.0003 0.0001 0.0024 0.0002 0.0001 0 0 0.0004
Nucleus 0.0135 0.0028 0.0007 0.0003 0.0725 0.0009 0.0032 0.0003 0.0003 0.0026 0.0055 0.0003 0.0071 0.0002 0.0001 0.0001 0 0.0012
Peroxisomes 0.011 0.0002 0.0034 0.0023 0.001 0.0013 0.016 0.0006 0.0019 0.0228 0.0354 0.0009 0.0145 0.0001 0.0053 0.0077 0 0.0011
Punctate Nuclear 0.0166 0.0033 0.0036 0.0017 0.0072 0.0054 0.0041 0.0003 0.0009 0.0032 0.0009 0.0003 0.0209 0.0002 0.0009 0.0004 0.0001 0.0016
Vacuole 0.0069 0.0006 0.0005 0.0004 0.0044 0.0005 0.0127 0.0005 0.0005 0.0216 0.0023 0.0002 0.0013 0.0002 0.0002 0.0002 0 0.0009
Vacuole Periphery 0.0024 0.0001 0.0002 0.0002 0.0016 0.0005 0.0021 0.0001 0.0002 0.0011 0.0057 0.0002 0.0006 0 0 0 0 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1154 1400 1537 878 487 1376 1352 1135 1641 2776 2889 2013

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 567.34 617.03 724.80 670.67 464.29 693.90 598.01 703.03 536.76 655.13 665.46 688.92
Standard Deviation 66.28 98.89 131.77 139.83 80.60 83.31 173.49 126.21 85.05 99.24 165.30 133.29
Intensity Change Log 2 — 0.121127 0.353369 0.241389 — 0.579702 0.365143 0.598560 — 0.345665 0.358680 0.413141

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000111 0.000212 0.000187 0.000669 0.000019 0.000296 0.000182 0.000342 0.000084 0.000254 0.000185 0.000485
Bud Neck 0.016998 0.025888 0.006710 0.007700 0.005778 0.050970 0.016144 0.008315 0.013668 0.038320 0.011125 0.008047
Bud Site 0.004886 0.009887 0.012204 0.014326 0.000368 0.010691 0.014219 0.016021 0.003545 0.010286 0.013147 0.015282
Cell Periphery 0.000167 0.000140 0.000220 0.000253 0.000757 0.000184 0.001743 0.000198 0.000342 0.000162 0.000933 0.000222
Cytoplasm 0.496823* 0.471422* 0.376724* 0.510283* 0.473528* 0.363527* 0.458589* 0.544093* 0.489909* 0.417941* 0.415035* 0.529346*
Cytoplasmic Foci 0.226086* 0.199810* 0.018359 0.052397 0.158050 0.306237* 0.047274 0.058046 0.205895* 0.252564* 0.031891 0.055582
Eisosomes 0.000207 0.000113 0.000064 0.000086 0.004259 0.000261 0.002554 0.000093 0.001409 0.000187 0.001229 0.000090
Endoplasmic Reticulum 0.000633 0.000495 0.001953 0.002255 0.001246 0.000468 0.002361 0.000874 0.000815 0.000481 0.002144 0.001476
Endosome 0.006061 0.016312 0.001920 0.005731 0.004497 0.024845 0.003204 0.005670 0.005597 0.020542 0.002521 0.005696
Golgi 0.002779 0.003076 0.000185 0.003653 0.002461 0.005456 0.001899 0.001842 0.002685 0.004255 0.000987 0.002632
Lipid Particles 0.008705 0.009103 0.001563 0.002192 0.021951 0.009762 0.009315 0.002422 0.012636 0.009430 0.005191 0.002321
Mitochondria 0.004245 0.010622 0.001018 0.001838 0.001838 0.017245 0.002006 0.002208 0.003531 0.013905 0.001481 0.002047
Mitotic Spindle 0.000363 0.007342 0.001528 0.009169 0.000057 0.006346 0.007359 0.006588 0.000272 0.006848 0.004257 0.007714
None 0.010202 0.012620 0.021281 0.021335 0.047670 0.001769 0.084691 0.013082 0.021321 0.007241 0.050956 0.016681
Nuclear Periphery 0.000316 0.000477 0.001270 0.001079 0.001039 0.000485 0.000956 0.000830 0.000530 0.000481 0.001123 0.000938
Nuclear Periphery Foci 0.000259 0.000628 0.000749 0.001124 0.000315 0.000182 0.001161 0.000503 0.000275 0.000407 0.000942 0.000774
Nucleolus 0.001884 0.003202 0.001196 0.001064 0.002591 0.001445 0.002351 0.001082 0.002094 0.002331 0.001737 0.001074
Nucleus 0.189219* 0.167261* 0.521926* 0.272599* 0.254633* 0.105647 0.306626* 0.243372* 0.208632* 0.136720* 0.421169* 0.256120*
Peroxisomes 0.004412 0.011268 0.000738 0.001602 0.003373 0.026069 0.001765 0.001820 0.004103 0.018605 0.001219 0.001725
Vacuole 0.024923 0.048110 0.029961 0.088114 0.015371 0.066304 0.034903 0.092133 0.022089 0.057129 0.032273 0.090380
Vacuole Periphery 0.000722 0.002010 0.000242 0.002531 0.000200 0.001812 0.000698 0.000469 0.000567 0.001912 0.000455 0.001369

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.83 -2.41 -2.60 -2.03 -2.03 -5.79 -2.45 -5.05 -1.17 -1.44 -5.09 -2.83 -4.38 -2.42 -2.60
Bud Neck -3.46 4.86 4.69 8.82 0.02 -15.21 -4.84 -1.68 14.22 3.63 -11.40 1.77 4.20 16.58 2.74
Bud Site -3.00 -2.98 -2.84 -0.54 0.06 -9.77 -6.48 -6.56 -0.95 0.26 -6.12 -5.66 -5.53 -1.11 0.13
Cell Periphery 1.96 -1.86 -1.11 -2.03 0.10 6.82 -4.16 6.97 0.70 8.64 6.47 -5.96 5.04 -1.24 8.35
Cytoplasm 2.65 13.55 5.22 2.91 -6.43 8.10 2.27 0.69 -8.50 -1.78 8.75 11.46 4.45 -3.82 -6.40
Cytoplasmic Foci 2.98 33.32 27.66 25.78 -7.53 -14.55 14.65 13.90 36.62 -1.44 -7.02 34.64 30.58 43.33 -6.57
Eisosomes 6.38 6.88 8.36 3.69 -1.37 4.85 2.39 5.05 9.20 5.98 4.91 1.29 5.31 9.70 5.84
Endoplasmic Reticulum 0.86 -9.16 -9.53 -11.73 -2.28 2.82 -3.00 -0.96 -9.21 3.05 2.41 -8.33 -8.40 -14.80 1.30
Endosome -7.66 7.21 2.65 9.18 -3.85 -11.25 1.40 0.18 12.66 -1.74 -13.85 6.12 2.12 15.42 -3.70
Golgi -0.38 5.49 0.81 1.09 -1.97 -3.51 1.52 1.80 4.92 0.79 -2.85 4.81 1.95 4.30 -1.23
Lipid Particles -0.43 9.71 9.08 8.13 -2.30 5.16 5.44 8.58 12.20 5.33 3.16 7.95 12.10 13.59 4.69
Mitochondria -4.86 4.29 3.65 8.50 -0.65 -8.38 0.13 0.56 8.44 0.39 -8.87 3.51 3.26 11.46 -0.00
Mitotic Spindle -3.94 -2.52 -4.69 -0.96 -3.81 -4.32 -5.25 -3.82 0.54 1.46 -5.72 -5.71 -5.95 -0.27 -1.65
None -0.84 -2.78 -1.12 -0.30 1.47 5.51 -2.32 4.58 -4.80 10.63 5.03 -5.33 3.60 -2.31 9.91
Nuclear Periphery -4.34 -20.99 -13.99 -12.14 -2.35 2.55 -0.98 -0.76 -6.45 0.58 0.54 -11.72 -11.10 -12.99 -0.99
Nuclear Periphery Foci -2.01 -5.86 -10.19 -2.77 -3.05 2.88 -5.77 -5.30 -9.30 3.35 -1.39 -8.30 -11.67 -4.53 0.70
Nucleolus -2.07 1.64 2.26 5.75 1.67 1.81 0.84 2.68 2.99 4.58 -0.47 1.44 3.25 6.38 4.48
Nucleus 2.83 -36.28 -10.62 -12.96 20.31 14.52 -6.30 -1.11 -19.42 5.95 12.19 -30.65 -9.75 -22.10 19.58
Peroxisomes -5.92 5.42 4.66 10.52 -0.99 -11.57 2.79 3.05 13.43 0.62 -12.51 5.84 5.46 16.66 -0.59
Vacuole -9.54 -6.02 -19.14 -15.54 -16.26 -15.46 -10.66 -22.05 -13.94 -16.42 -16.68 -10.71 -28.60 -20.91 -23.19
Vacuole Periphery -4.32 4.39 -0.91 0.76 -1.61 -7.50 -1.18 -0.84 5.99 0.79 -7.03 0.96 -0.64 3.02 -1.06
Standard name
Human Ortholog
Description Protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (33%), nucleus (4%), mixed (38%)
Cell Cycle Regulation Yes
cytoplasm - S/G2 (0.00031)
cytoplasm - MA (5.6e-05)
nucleus - T/G1 (1.4e-05)
Subcompartmental Group N/A

Cis1

Cis1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cis1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available