Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 0 | 1 | 13 | 22 | 20 | – | 19 | 20 | 21 | 33 | 5 | 3 | 3 | 4 | 1 | 0 | 2 | 3 | 3 | 1 | 1 | 2 |
Bud | 1 | 5 | 2 | 1 | 2 | 3 | 7 | – | 0 | 8 | 1 | 3 | 7 | 0 | 2 | 1 | 3 | 1 | 3 | 7 | 5 | 2 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 0 | 0 | 0 | 2 | – | 3 | 4 | 3 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 215 | 179 | 144 | 95 | 93 | 112 | 147 | – | 110 | 134 | 63 | 94 | 144 | 191 | 218 | 83 | 62 | 78 | 118 | 160 | 130 | 67 | 115 | 142 |
Endoplasmic Reticulum | 74 | 38 | 59 | 40 | 53 | 63 | 88 | – | 71 | 65 | 32 | 74 | 84 | 57 | 74 | 4 | 3 | 3 | 129 | 22 | 74 | 59 | 88 | 93 |
Endosome | 0 | 0 | 1 | 0 | 2 | 8 | 5 | – | 0 | 1 | 0 | 2 | 1 | 0 | 10 | 0 | 2 | 2 | 12 | 26 | 12 | 7 | 12 | 24 |
Golgi | 12 | 1 | 6 | 18 | 71 | 105 | 61 | – | 42 | 52 | 23 | 63 | 19 | 26 | 46 | 98 | 69 | 51 | 6 | 1 | 1 | 4 | 16 | 29 |
Mitochondria | 0 | 2 | 0 | 0 | 1 | 2 | 2 | – | 13 | 6 | 13 | 8 | 0 | 1 | 0 | 7 | 1 | 1 | 1 | 1 | 1 | 0 | 2 | 2 |
Nucleus | 1 | 1 | 1 | 1 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 17 | 4 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 51 | 33 | 11 | 10 | 13 |
Nucleolus | 8 | 4 | 3 | 0 | 11 | 17 | 21 | – | 10 | 11 | 8 | 10 | 5 | 5 | 19 | 16 | 15 | 6 | 1 | 2 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 1 | 1 | 2 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 7 | 5 | 3 | 3 | 5 | 5 |
Vac/Vac Membrane | 37 | 42 | 58 | 33 | 68 | 64 | 64 | – | 33 | 62 | 62 | 66 | 21 | 28 | 56 | 80 | 66 | 65 | 40 | 68 | 42 | 10 | 22 | 31 |
Unique Cell Count | 326 | 264 | 253 | 178 | 268 | 340 | 363 | 263 | 329 | 206 | 295 | 258 | 293 | 401 | 252 | 186 | 179 | 438 | 380 | 322 | 181 | 289 | 358 | |
Labelled Cell Count | 349 | 273 | 280 | 190 | 316 | 398 | 418 | 302 | 364 | 232 | 359 | 286 | 311 | 429 | 299 | 223 | 207 | 438 | 380 | 322 | 181 | 289 | 358 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 39.8 | 31.1 | 32.9 | 30.9 | 31.5 | 31.3 | 28.8 | – | 27.8 | 26.4 | 24.2 | 27.0 | 34.8 | 37.5 | 34.3 | 47.0 | 52.1 | 45.6 | 34.0 | 34.8 | 35.5 |
Std Deviation (1e-4) | 8.5 | 8.4 | 8.1 | 5.8 | 5.8 | 6.5 | 6.9 | – | 6.6 | 6.3 | 6.7 | 7.1 | 7.2 | 8.1 | 8.1 | 15.6 | 15.6 | 15.0 | 6.9 | 7.1 | 8.3 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.06 | -0.07 | -0.19 | – | -0.25 | -0.32 | -0.44 | -0.28 | 0.08 | 0.19 | 0.06 | 0.52 | 0.66 | 0.47 | 0.05 | 0.08 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 3.5 | 4.1 | 3.8 | – | 4.4 | 4.0 | 5.2 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -5.1 | -5.8 | -4.0 | – | -3.4 | -3.9 | -5.6 | -5.9 | -0.3 | 2.0 | -0.6 | -5.4 | -4.9 | -2.7 |
Endoplasmic Reticulum | -0.2 | -1.0 | -1.4 | 0.3 | – | 1.0 | -1.0 | -2.1 | 0.5 | 2.3 | -1.1 | -1.5 | -7.4 | -6.5 | -6.3 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 3.5 | 7.8 | 8.8 | 5.7 | – | 5.3 | 5.4 | 3.9 | 6.7 | 2.6 | 3.2 | 4.2 | 10.1 | 9.6 | 7.9 |
Mitochondria | 0 | 0 | 0 | 0 | – | 3.6 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.1 | 2.5 | 2.9 | – | 1.9 | 1.7 | 0 | 1.7 | 0 | 0 | 2.5 | 3.0 | 3.6 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.1 | 0.7 | -1.2 | -1.6 | – | -3.1 | -1.2 | 1.7 | -0.2 | -4.6 | -4.3 | -2.9 | 2.2 | 2.9 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.268 | 40.2542 | 33.4087 | 32.715 | 34.5504 | 35.6133 | 30.698 | 43.6043 | 38.6632 | 34.8645 | 37.6641 | 38.5315 | 31.3159 | 45.3247 | 39.8152 | 37.5641 | 29.9672 | 40.6356 |
Actin | 0.019 | 0.0103 | 0.009 | 0.0011 | 0.0016 | 0.0005 | 0.0219 | 0.0005 | 0.004 | 0.0137 | 0.0006 | 0.0086 | 0.0159 | 0.0003 | 0.0162 | 0.0039 | 0.0231 | 0.0016 |
Bud | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0053 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0005 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 |
Bud Site | 0.0014 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0015 | 0 | 0.0004 | 0.0008 | 0 | 0.0001 | 0.0027 | 0 | 0.0009 | 0.0002 | 0.0006 | 0 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0567 | 0.0709 | 0.0499 | 0.1523 | 0.0627 | 0.0367 | 0.0397 | 0.1138 | 0.0611 | 0.0945 | 0.1073 | 0.0711 | 0.0449 | 0.0836 | 0.0917 | 0.2052 | 0.0597 | 0.1315 |
Cytoplasmic Foci | 0.0156 | 0.0104 | 0.0121 | 0.0485 | 0.0357 | 0.0208 | 0.0136 | 0.0104 | 0.013 | 0.015 | 0.0342 | 0.0145 | 0.0161 | 0.0063 | 0.0157 | 0.0093 | 0.0551 | 0.0071 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0469 | 0.0351 | 0.0293 | 0.0819 | 0.054 | 0.0261 | 0.0163 | 0.0205 | 0.0148 | 0.0501 | 0.0093 | 0.0292 | 0.0092 | 0.0142 | 0.0093 | 0.0175 | 0.0121 | 0.0159 |
Endosome | 0.0239 | 0.0353 | 0.0312 | 0.0672 | 0.0599 | 0.0395 | 0.0202 | 0.0127 | 0.0233 | 0.1043 | 0.1266 | 0.0305 | 0.0146 | 0.0087 | 0.02 | 0.0239 | 0.0523 | 0.0184 |
Golgi | 0.0057 | 0.004 | 0.0057 | 0.0086 | 0.0038 | 0.0089 | 0.007 | 0.0035 | 0.0061 | 0.0171 | 0.0138 | 0.0089 | 0.0058 | 0.0007 | 0.0033 | 0.0144 | 0.0314 | 0.0012 |
Lipid Particles | 0.0313 | 0.0431 | 0.0332 | 0.0659 | 0.0171 | 0.0418 | 0.0149 | 0.0265 | 0.0586 | 0.0348 | 0.0219 | 0.0144 | 0.028 | 0.0123 | 0.0101 | 0.0275 | 0.1461 | 0.0067 |
Mitochondria | 0.005 | 0.0002 | 0.0014 | 0.0011 | 0.0025 | 0.0003 | 0.0037 | 0.0002 | 0.0029 | 0.0377 | 0.0009 | 0.0009 | 0.0055 | 0.0001 | 0.0029 | 0.0253 | 0.0245 | 0.0003 |
None | 0.0127 | 0.0002 | 0.0013 | 0.003 | 0.0005 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0027 | 0.0002 | 0.0026 | 0.0005 | 0.0052 | 0.0003 |
Nuclear Periphery | 0.6786 | 0.7282 | 0.7574 | 0.4888 | 0.6852 | 0.7514 | 0.7843 | 0.7456 | 0.7431 | 0.5567 | 0.6179 | 0.757 | 0.7692 | 0.7749 | 0.7458 | 0.6264 | 0.4826 | 0.6986 |
Nucleolus | 0.0028 | 0.0004 | 0.0009 | 0.0016 | 0.0137 | 0.0006 | 0.0007 | 0.0025 | 0.0076 | 0.0003 | 0.0007 | 0.0004 | 0.0013 | 0.0034 | 0.0007 | 0.0004 | 0.0014 | 0.0002 |
Nucleus | 0.0384 | 0.0251 | 0.0282 | 0.0196 | 0.024 | 0.0351 | 0.0373 | 0.0373 | 0.0288 | 0.0173 | 0.0282 | 0.0283 | 0.0533 | 0.0735 | 0.0427 | 0.02 | 0.0698 | 0.0908 |
Peroxisomes | 0.0193 | 0.0002 | 0.0021 | 0.0003 | 0.0013 | 0.0002 | 0.0042 | 0.0001 | 0.0004 | 0.0006 | 0.0001 | 0.0004 | 0.0033 | 0.0001 | 0.0023 | 0.0008 | 0.0074 | 0.0001 |
Punctate Nuclear | 0.0118 | 0.0044 | 0.0044 | 0.0128 | 0.0034 | 0.005 | 0.0106 | 0.011 | 0.01 | 0.0023 | 0.0029 | 0.0049 | 0.01 | 0.0091 | 0.0174 | 0.0033 | 0.011 | 0.009 |
Vacuole | 0.0093 | 0.0087 | 0.0086 | 0.0264 | 0.0096 | 0.0083 | 0.0065 | 0.0033 | 0.0066 | 0.0163 | 0.0096 | 0.0091 | 0.0029 | 0.0025 | 0.0041 | 0.0044 | 0.0028 | 0.0056 |
Vacuole Periphery | 0.0202 | 0.0228 | 0.0235 | 0.0203 | 0.0246 | 0.0243 | 0.0158 | 0.0115 | 0.0184 | 0.0358 | 0.0251 | 0.0206 | 0.0086 | 0.0098 | 0.0131 | 0.0164 | 0.0133 | 0.012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 81.9233 | 79.3373 | 75.2878 | 86.3277 | 86.8546 | 66.5258 | 67.8766 | 71.3212 | 70.6537 | 83.7971 |
Translational Efficiency | 1.2881 | 1.2735 | 1.244 | 1.0377 | 1.1174 | 1.3059 | 1.2378 | 1.0725 | 1.0774 | 1.0035 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1196 | 1450 | 2082 | 1224 | 1673 | 1274 | 1561 | 2170 | 2869 | 2724 | 3643 | 3394 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1997.31 | 2689.78 | 4746.60 | 4664.92 | 2185.45 | 2458.99 | 3263.39 | 4259.17 | 2107.02 | 2581.84 | 4111.05 | 4405.50 |
Standard Deviation | 342.94 | 525.05 | 871.76 | 920.08 | 451.63 | 541.34 | 955.96 | 1035.12 | 420.21 | 545.03 | 1168.18 | 1014.06 |
Intensity Change Log 2 | — | 0.429430 | 1.248836 | 1.223794 | — | 0.170135 | 0.578441 | 0.962642 | — | 0.299773 | 0.937345 | 1.093250 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000106 | 0.000073 | 0.000663 | 0.000423 | 0.000206 | 0.000224 | 0.000108 | 0.000198 | 0.000164 | 0.000144 | 0.000425 | 0.000279 |
Bud Neck | 0.004520 | 0.001820 | 0.008574 | 0.007346 | 0.003089 | 0.003407 | 0.004948 | 0.004577 | 0.003686 | 0.002562 | 0.007020 | 0.005575 |
Bud Site | 0.002350 | 0.000610 | 0.004551 | 0.009123 | 0.001351 | 0.002214 | 0.002580 | 0.006618 | 0.001768 | 0.001360 | 0.003706 | 0.007521 |
Cell Periphery | 0.000122 | 0.000095 | 0.000238 | 0.001493 | 0.000177 | 0.000203 | 0.000161 | 0.000460 | 0.000154 | 0.000146 | 0.000205 | 0.000833 |
Cytoplasm | 0.066470 | 0.079223 | 0.047309 | 0.190808* | 0.114129* | 0.090327 | 0.207788* | 0.243611* | 0.094261 | 0.084416 | 0.116073* | 0.224569* |
Cytoplasmic Foci | 0.017112 | 0.002499 | 0.018736 | 0.026552 | 0.006871 | 0.004808 | 0.003553 | 0.014194 | 0.011140 | 0.003579 | 0.012230 | 0.018651 |
Eisosomes | 0.000004 | 0.000001 | 0.000006 | 0.000004 | 0.000002 | 0.000002 | 0.000001 | 0.000004 | 0.000003 | 0.000001 | 0.000004 | 0.000004 |
Endoplasmic Reticulum | 0.009711 | 0.003369 | 0.004262 | 0.003143 | 0.009280 | 0.005342 | 0.003157 | 0.005238 | 0.009460 | 0.004292 | 0.003788 | 0.004483 |
Endosome | 0.011476 | 0.003277 | 0.016187 | 0.010998 | 0.007446 | 0.006961 | 0.003625 | 0.009483 | 0.009126 | 0.005000 | 0.010804 | 0.010029 |
Golgi | 0.005082 | 0.002853 | 0.017455 | 0.028205 | 0.006287 | 0.005070 | 0.002408 | 0.019844 | 0.005785 | 0.003890 | 0.011007 | 0.022859 |
Lipid Particles | 0.006164 | 0.002770 | 0.033329 | 0.050541 | 0.006901 | 0.005431 | 0.004023 | 0.023738 | 0.006593 | 0.004014 | 0.020772 | 0.033404 |
Mitochondria | 0.000404 | 0.000521 | 0.000814 | 0.000493 | 0.000451 | 0.000839 | 0.000654 | 0.000770 | 0.000432 | 0.000670 | 0.000745 | 0.000670 |
Mitotic Spindle | 0.049078 | 0.056882 | 0.150802* | 0.134086* | 0.033237 | 0.062740 | 0.082071 | 0.117754* | 0.039841 | 0.059622 | 0.121351* | 0.123644* |
None | 0.001289 | 0.001673 | 0.000854 | 0.002371 | 0.002086 | 0.003808 | 0.001152 | 0.002281 | 0.001754 | 0.002672 | 0.000982 | 0.002313 |
Nuclear Periphery | 0.083504 | 0.165224* | 0.105601* | 0.021543 | 0.071959 | 0.149801* | 0.180360* | 0.036712 | 0.076772 | 0.158011* | 0.137635* | 0.031242 |
Nuclear Periphery Foci | 0.714618* | 0.653394* | 0.539441* | 0.351796* | 0.710950* | 0.622334* | 0.432649* | 0.403947* | 0.712479* | 0.638867* | 0.493681* | 0.385140* |
Nucleolus | 0.003655 | 0.000239 | 0.000209 | 0.000256 | 0.000701 | 0.000840 | 0.000276 | 0.000449 | 0.001932 | 0.000520 | 0.000237 | 0.000379 |
Nucleus | 0.007616 | 0.010902 | 0.004200 | 0.009814 | 0.008330 | 0.012504 | 0.025807 | 0.025582 | 0.008032 | 0.011651 | 0.013458 | 0.019896 |
Peroxisomes | 0.000577 | 0.000010 | 0.000354 | 0.000241 | 0.000134 | 0.000119 | 0.000041 | 0.000200 | 0.000318 | 0.000061 | 0.000220 | 0.000215 |
Vacuole | 0.012193 | 0.010172 | 0.038899 | 0.140797 | 0.013040 | 0.017805 | 0.041343 | 0.077538 | 0.012687 | 0.013742 | 0.039946 | 0.100352 |
Vacuole Periphery | 0.003949 | 0.004394 | 0.007517 | 0.009966 | 0.003372 | 0.005220 | 0.003294 | 0.006804 | 0.003612 | 0.004780 | 0.005708 | 0.007944 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.89 | -2.95 | -3.00 | -3.16 | 1.34 | -0.95 | 0.39 | -0.25 | 1.02 | -1.25 | -0.45 | -2.49 | -2.13 | -1.87 | 1.50 |
Bud Neck | 4.26 | -6.51 | -6.80 | -12.05 | 0.35 | -1.41 | -6.34 | -8.19 | -7.01 | -1.10 | 2.00 | -9.41 | -10.43 | -13.39 | 0.27 |
Bud Site | 3.44 | -3.97 | -6.36 | -8.16 | -4.17 | -2.06 | -2.92 | -8.76 | -6.25 | -4.51 | 0.53 | -5.39 | -10.61 | -10.97 | -6.00 |
Cell Periphery | 1.18 | -8.51 | -12.25 | -12.40 | -10.75 | -1.12 | -2.27 | -7.62 | -4.98 | -5.78 | -0.10 | -6.77 | -14.29 | -13.67 | -11.90 |
Cytoplasm | -2.09 | 2.86 | -14.87 | -13.24 | -18.34 | 2.45 | -12.73 | -18.16 | -20.85 | -3.78 | 1.26 | -6.15 | -23.84 | -25.61 | -18.47 |
Cytoplasmic Foci | 6.46 | -1.25 | -3.07 | -10.29 | -2.26 | 1.34 | 1.53 | -5.10 | -6.76 | -7.32 | 6.12 | -1.80 | -5.20 | -12.58 | -3.75 |
Eisosomes | 4.45 | -1.92 | 0.12 | -9.74 | 2.71 | -1.43 | 0.47 | -4.53 | -2.45 | -6.72 | 2.64 | -2.54 | -2.79 | -7.33 | 0.28 |
Endoplasmic Reticulum | 5.06 | 4.22 | 5.82 | 1.52 | 2.76 | 4.09 | 7.72 | 5.36 | 1.74 | -2.19 | 6.85 | 7.84 | 7.68 | 1.58 | 0.17 |
Endosome | 8.74 | -3.18 | 1.82 | -9.27 | 5.36 | 0.29 | 6.36 | -1.92 | -1.90 | -8.26 | 7.07 | -2.23 | 0.19 | -7.66 | 2.53 |
Golgi | 2.52 | -8.98 | -9.69 | -11.72 | -2.41 | 1.27 | 5.41 | -6.44 | -7.26 | -10.83 | 2.92 | -6.12 | -11.07 | -13.47 | -5.70 |
Lipid Particles | 3.77 | -11.80 | -15.98 | -17.12 | -5.08 | 1.01 | 1.37 | -9.37 | -9.77 | -10.39 | 3.34 | -10.73 | -17.69 | -19.19 | -5.92 |
Mitochondria | -1.41 | -4.01 | -0.20 | 0.88 | 3.13 | -3.58 | -3.48 | -1.49 | 0.68 | 0.42 | -3.48 | -5.02 | -1.52 | 0.47 | 1.46 |
Mitotic Spindle | -1.70 | -15.31 | -10.91 | -9.58 | 2.06 | -6.16 | -10.46 | -14.99 | -7.40 | -3.08 | -5.82 | -19.60 | -18.20 | -12.05 | 0.85 |
None | -1.32 | 1.86 | -1.69 | -0.83 | -2.49 | -1.88 | 2.25 | -1.11 | 1.44 | -3.60 | -2.02 | 3.39 | -2.13 | 0.60 | -4.79 |
Nuclear Periphery | -13.43* | -3.93 | 17.20 | 29.62* | 24.96 | -13.27 | -14.42 | 14.25 | 22.28* | 23.70* | -19.47 | -13.34 | 21.64 | 35.95* | 32.67* |
Nuclear Periphery Foci | 5.68 | 17.69 | 34.77* | 30.31* | 19.43 | 8.77 | 30.49* | 38.18* | 25.33* | 5.66 | 10.10 | 32.50 | 51.26 | 39.41* | 18.77 |
Nucleolus | 3.62 | 3.62 | 3.51 | -1.43 | -1.44 | -0.66 | 0.87 | -1.12 | -0.51 | -3.45 | 3.12 | 3.73 | 2.56 | -1.59 | -4.02 |
Nucleus | -2.32 | 1.37 | -4.32 | -1.69 | -6.18 | -3.31 | -11.24 | -14.04 | -10.95 | -3.03 | -3.92 | -8.44 | -14.57 | -11.26 | -7.89 |
Peroxisomes | 3.12 | 1.16 | 2.03 | -3.70 | 1.48 | -0.11 | 0.86 | -1.15 | -1.59 | -3.92 | 2.56 | 0.93 | 0.88 | -3.77 | -0.18 |
Vacuole | 0.73 | -13.52 | -25.65 | -25.96 | -19.33 | -3.63 | -14.42 | -26.61 | -22.68 | -11.45 | -2.15 | -19.79 | -36.59 | -34.98 | -22.27 |
Vacuole Periphery | -0.46 | -4.79 | -4.78 | -3.64 | -0.46 | -2.23 | 0.32 | -3.99 | -0.46 | -4.78 | -1.83 | -4.90 | -5.99 | -2.65 | -1.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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