Standard name | |
---|---|
Human Ortholog | |
Description | Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 3 | 7 | 2 | 12 | 26 | 9 | 13 | 16 | 9 | 10 | 17 | 4 | 11 | 15 | 6 | 14 | 8 | 5 | 4 | 8 | 9 | 10 | 9 |
Bud | 1 | 1 | 3 | 0 | 9 | 13 | 18 | 16 | 14 | 14 | 12 | 20 | 0 | 2 | 2 | 3 | 2 | 1 | 5 | 0 | 0 | 2 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 5 | 3 | 20 | 23 | 16 | 15 | 9 | 19 | 15 | 25 | 31 | 27 | 54 | 100 | 33 | 28 | 35 | 0 | 1 | 2 | 2 | 2 | 4 |
Cytoplasm | 47 | 35 | 106 | 94 | 138 | 189 | 202 | 223 | 170 | 146 | 150 | 156 | 106 | 97 | 170 | 127 | 78 | 74 | 17 | 9 | 14 | 8 | 11 | 17 |
Endoplasmic Reticulum | 154 | 128 | 158 | 106 | 162 | 191 | 154 | 104 | 112 | 119 | 128 | 151 | 220 | 392 | 374 | 3 | 3 | 2 | 209 | 146 | 301 | 295 | 389 | 461 |
Endosome | 0 | 1 | 3 | 1 | 5 | 2 | 2 | 5 | 1 | 4 | 1 | 2 | 5 | 1 | 1 | 3 | 0 | 1 | 3 | 0 | 0 | 1 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 | 0 | 1 | 1 | 1 | 0 | 1 | 6 | 0 |
Mitochondria | 3 | 0 | 25 | 34 | 53 | 80 | 100 | 95 | 136 | 115 | 139 | 164 | 1 | 1 | 1 | 7 | 3 | 0 | 4 | 4 | 10 | 8 | 11 | 7 |
Nucleus | 2 | 0 | 2 | 2 | 1 | 6 | 2 | 4 | 6 | 6 | 3 | 6 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 3 | 4 | 8 | 1 | 6 | 6 | 16 | 5 | 12 | 9 | 10 | 1 | 4 | 7 | 2 | 1 | 0 | 1 | 0 | 6 | 1 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 28 | 3 | 22 | 13 | 29 | 24 | 35 | 91 | 65 | 72 | 41 | 41 | 48 | 30 | 68 | 31 | 11 | 6 | 7 | 4 | 7 | 11 | 15 | 18 |
Unique Cell Count | 221 | 163 | 295 | 232 | 361 | 469 | 455 | 503 | 452 | 443 | 421 | 486 | 358 | 512 | 614 | 205 | 128 | 121 | 261 | 180 | 357 | 348 | 459 | 535 |
Labelled Cell Count | 245 | 179 | 333 | 280 | 433 | 555 | 543 | 579 | 548 | 515 | 519 | 599 | 413 | 593 | 742 | 224 | 141 | 128 | 261 | 180 | 357 | 348 | 459 | 535 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 8.7 | 6.6 | 6.8 | 6.4 | 6.3 | 5.9 | 6.2 | 6.2 | 6.2 | 6.3 | 6.4 | 10.6 | 10.2 | 9.6 | 10.8 | 11.0 | 11.5 | 11.7 | 13.2 | 13.8 |
Std Deviation (1e-4) | 1.6 | 1.5 | 1.1 | 1.9 | 1.4 | 1.4 | 1.4 | 1.5 | 1.6 | 1.6 | 1.8 | 1.7 | 2.2 | 2.0 | 1.6 | 1.8 | 2.0 | 2.0 | 2.6 | 2.7 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.04 | -0.06 | -0.16 | -0.09 | -0.09 | -0.09 | -0.05 | -0.04 | 0.68 | 0.63 | 0.55 | 0.72 | 0.74 | 0.8 | 0.83 | 1.0 | 1.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.7 | 2.1 | -0.4 | 0.2 | 0.9 | -0.3 | 0 | 0.9 | 0 | -0.2 | 0.1 | 0.4 | 3.7 | 0 |
Bud | 0 | 1.4 | 1.6 | 2.4 | 1.9 | 1.9 | 1.9 | 1.7 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 4.2 | 3.5 | 2.1 | 2.0 | 0 | 2.5 | 2.0 | 3.3 | 3.6 | 4.0 | 5.1 | 6.8 | 6.4 | 7.6 | 9.0 |
Cytoplasm | 1.1 | 0.6 | 1.2 | 2.3 | 2.3 | 0.5 | -0.8 | -0.1 | -1.1 | -1.7 | -5.4 | -2.5 | 5.7 | 4.8 | 4.7 |
Endoplasmic Reticulum | -1.8 | -2.2 | -3.5 | -5.4 | -9.5 | -8.0 | -7.3 | -6.2 | -6.2 | 2.0 | 6.8 | 2.1 | -12.3 | -10.0 | -9.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.2 | 2.4 | 3.4 | 4.8 | 4.0 | 7.0 | 5.9 | 7.7 | 8.0 | -5.3 | -6.4 | -7.0 | -2.3 | -2.3 | -3.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.6 | 0 | 0 | 0 | 1.6 | 0 | 1.2 | 0.8 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | 0.3 | -1.3 | 0.1 | 4.2 | 2.9 | 3.5 | 1.1 | 0.5 | 2.4 | -0.9 | 1.7 | 2.7 | 0.4 | -0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.22 | 5.365 | 5.4868 | 5.2706 | 5.0482 | 5.7628 | 6.7719 | 7.2229 | 6.8943 | 6.6733 | 7.1353 | 6.6316 | 5.5982 | 4.2918 | 3.3242 | 4.1289 | 4.1431 | 4.0326 |
Actin | 0.0244 | 0.0057 | 0.0208 | 0.0058 | 0.0137 | 0.0009 | 0.0425 | 0.0021 | 0.0129 | 0.0026 | 0.0302 | 0.0007 | 0.0276 | 0.0004 | 0.0003 | 0.0733 | 0.0009 | 0.0111 |
Bud | 0.0006 | 0.0035 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.0023 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0 |
Bud Neck | 0.0028 | 0.0008 | 0.0006 | 0.0038 | 0.0008 | 0.0015 | 0.0065 | 0.0004 | 0.0007 | 0.0005 | 0.0093 | 0.0004 | 0.0019 | 0.0001 | 0.0001 | 0.001 | 0.0093 | 0.0003 |
Bud Periphery | 0.0011 | 0.0027 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0014 | 0.0003 | 0.0005 | 0.0011 | 0.0028 | 0 | 0.0008 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 |
Bud Site | 0.0051 | 0.0113 | 0.0011 | 0.0019 | 0.0005 | 0 | 0.0016 | 0.0029 | 0.0048 | 0.0009 | 0.0121 | 0 | 0.002 | 0.0004 | 0 | 0.0013 | 0.0002 | 0.0001 |
Cell Periphery | 0.0017 | 0.0024 | 0.001 | 0.0007 | 0.0005 | 0.0002 | 0.0035 | 0.0086 | 0.001 | 0.0009 | 0.0009 | 0.0006 | 0.0011 | 0.0012 | 0.0005 | 0.0003 | 0.0027 | 0.0001 |
Cytoplasm | 0.1325 | 0.3432 | 0.1667 | 0.225 | 0.126 | 0.1108 | 0.0818 | 0.1808 | 0.1732 | 0.1914 | 0.2806 | 0.1309 | 0.1065 | 0.2314 | 0.0992 | 0.078 | 0.3547 | 0.124 |
Cytoplasmic Foci | 0.0243 | 0.0095 | 0.0179 | 0.0043 | 0.0057 | 0.0089 | 0.0168 | 0.0057 | 0.0058 | 0.0048 | 0.0155 | 0.0024 | 0.001 | 0.0022 | 0.0023 | 0.0014 | 0.005 | 0.0026 |
Eisosomes | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.5295 | 0.4664 | 0.6621 | 0.672 | 0.5469 | 0.8125 | 0.7108 | 0.7257 | 0.7353 | 0.707 | 0.5675 | 0.8122 | 0.8094 | 0.7149 | 0.8783 | 0.8331 | 0.6096 | 0.8088 |
Endosome | 0.0813 | 0.0193 | 0.035 | 0.0254 | 0.0567 | 0.0113 | 0.0193 | 0.0136 | 0.0186 | 0.0096 | 0.0352 | 0.0112 | 0.0042 | 0.0033 | 0.0039 | 0.0016 | 0.0041 | 0.0048 |
Golgi | 0.012 | 0.0024 | 0.0067 | 0.0069 | 0.0093 | 0.0009 | 0.0098 | 0.0013 | 0.0044 | 0.0011 | 0.0126 | 0.0008 | 0.0015 | 0.0003 | 0.0005 | 0.0029 | 0.0005 | 0.0095 |
Lipid Particles | 0.0321 | 0.0057 | 0.0125 | 0.0051 | 0.0179 | 0.0016 | 0.0258 | 0.0076 | 0.0062 | 0.0013 | 0.0038 | 0.0019 | 0.0013 | 0.0063 | 0.0044 | 0.0008 | 0.0034 | 0.0178 |
Mitochondria | 0.0041 | 0.0012 | 0.0031 | 0.0087 | 0.0034 | 0.0006 | 0.0075 | 0.0005 | 0.0052 | 0.0006 | 0.0025 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0014 |
None | 0.0258 | 0.0445 | 0.0245 | 0.0058 | 0.0121 | 0.0093 | 0.0037 | 0.0191 | 0.0115 | 0.0593 | 0.0034 | 0.0192 | 0.0264 | 0.0271 | 0.0022 | 0.0005 | 0.001 | 0.0004 |
Nuclear Periphery | 0.0546 | 0.0312 | 0.0258 | 0.0174 | 0.1069 | 0.0255 | 0.0203 | 0.017 | 0.0102 | 0.0103 | 0.0066 | 0.0143 | 0.0104 | 0.0073 | 0.0059 | 0.0031 | 0.0032 | 0.0072 |
Nucleolus | 0.0019 | 0.0016 | 0.0012 | 0.0004 | 0.0014 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0167 | 0.0256 | 0.0053 | 0.0052 | 0.0607 | 0.0069 | 0.0112 | 0.0029 | 0.0017 | 0.0024 | 0.0054 | 0.0018 | 0.0016 | 0.0015 | 0.0006 | 0.0003 | 0.0015 | 0.0033 |
Peroxisomes | 0.0045 | 0.0002 | 0.0023 | 0.0014 | 0.0013 | 0.0002 | 0.013 | 0.0001 | 0.0013 | 0.0002 | 0.0021 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0.0063 |
Punctate Nuclear | 0.0241 | 0.0094 | 0.006 | 0.0009 | 0.0213 | 0.0058 | 0.002 | 0.0013 | 0.0015 | 0.0005 | 0.0016 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0004 |
Vacuole | 0.017 | 0.0121 | 0.005 | 0.0066 | 0.0087 | 0.0021 | 0.0191 | 0.0093 | 0.0034 | 0.004 | 0.0049 | 0.002 | 0.0019 | 0.003 | 0.0014 | 0.0007 | 0.003 | 0.0015 |
Vacuole Periphery | 0.0034 | 0.001 | 0.0016 | 0.0015 | 0.0049 | 0.0005 | 0.0017 | 0.0005 | 0.0007 | 0.0005 | 0.0007 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.6785 | 37.8793 | 33.7297 | 32.5108 | 32.9634 | 37.268 | 41.3637 | 29.9857 | 32.9237 | 36.8555 |
Translational Efficiency | 1.7477 | 1.6075 | 1.4622 | 2.1575 | 1.8546 | 2.4587 | 1.5094 | 2.2699 | 1.9398 | 1.8098 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
815 | 1605 | 2005 | 256 | 1952 | 1949 | 2105 | 1828 | 2767 | 3554 | 4110 | 2084 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 874.97 | 955.22 | 1342.25 | 2497.25 | 880.37 | 875.65 | 1495.32 | 2291.84 | 878.78 | 911.58 | 1420.65 | 2317.07 |
Standard Deviation | 165.48 | 153.70 | 228.46 | 525.21 | 119.05 | 146.32 | 228.08 | 478.74 | 134.42 | 154.85 | 240.75 | 489.36 |
Intensity Change Log 2 | — | 0.126599 | 0.617348 | 1.513035 | — | -0.007756 | 0.764272 | 1.380324 | — | 0.060779 | 0.692905 | 1.448001 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001879 | 0.001011 | 0.001357 | 0.000798 | 0.000327 | 0.000632 | 0.001059 | 0.004172 | 0.000784 | 0.000803 | 0.001204 | 0.003757 |
Bud Neck | 0.002136 | 0.001662 | 0.002063 | 0.002605 | 0.001698 | 0.002902 | 0.001272 | 0.003613 | 0.001827 | 0.002342 | 0.001658 | 0.003489 |
Bud Site | 0.014673 | 0.007150 | 0.008260 | 0.004410 | 0.001548 | 0.005628 | 0.005461 | 0.013575 | 0.005414 | 0.006316 | 0.006827 | 0.012449 |
Cell Periphery | 0.007761 | 0.001687 | 0.002388 | 0.040888 | 0.000673 | 0.000995 | 0.002879 | 0.012930 | 0.002760 | 0.001308 | 0.002639 | 0.016365 |
Cytoplasm | 0.038677 | 0.122483* | 0.046272 | 0.002126 | 0.162163* | 0.156325* | 0.027674 | 0.006006 | 0.125791* | 0.141042* | 0.036747 | 0.005529 |
Cytoplasmic Foci | 0.017395 | 0.023353 | 0.001731 | 0.000768 | 0.024245 | 0.033297 | 0.001406 | 0.004615 | 0.022228 | 0.028806 | 0.001565 | 0.004142 |
Eisosomes | 0.000582 | 0.000453 | 0.000138 | 0.001189 | 0.000468 | 0.000430 | 0.000140 | 0.000354 | 0.000502 | 0.000441 | 0.000139 | 0.000456 |
Endoplasmic Reticulum | 0.861882* | 0.756469* | 0.888936* | 0.839180* | 0.713732* | 0.687560* | 0.931114* | 0.824810* | 0.757369* | 0.718680* | 0.910538* | 0.826575* |
Endosome | 0.005477 | 0.006612 | 0.007003 | 0.011930 | 0.004613 | 0.008353 | 0.004343 | 0.021497 | 0.004868 | 0.007567 | 0.005641 | 0.020321 |
Golgi | 0.006367 | 0.011401 | 0.001017 | 0.009963 | 0.009138 | 0.012189 | 0.001354 | 0.020785 | 0.008322 | 0.011833 | 0.001190 | 0.019456 |
Lipid Particles | 0.006797 | 0.016693 | 0.006834 | 0.033703 | 0.026384 | 0.026627 | 0.005114 | 0.047696 | 0.020615 | 0.022141 | 0.005953 | 0.045977 |
Mitochondria | 0.004927 | 0.005277 | 0.000626 | 0.000845 | 0.003656 | 0.009192 | 0.001312 | 0.002400 | 0.004030 | 0.007424 | 0.000977 | 0.002209 |
Mitotic Spindle | 0.000184 | 0.001436 | 0.000335 | 0.001166 | 0.000071 | 0.000485 | 0.000111 | 0.001956 | 0.000104 | 0.000914 | 0.000221 | 0.001859 |
None | 0.006927 | 0.005286 | 0.002101 | 0.000220 | 0.003522 | 0.005576 | 0.003462 | 0.001975 | 0.004525 | 0.005445 | 0.002798 | 0.001759 |
Nuclear Periphery | 0.000622 | 0.002442 | 0.002156 | 0.004579 | 0.006356 | 0.002907 | 0.000985 | 0.001357 | 0.004667 | 0.002697 | 0.001556 | 0.001753 |
Nuclear Periphery Foci | 0.004581 | 0.006085 | 0.013898 | 0.008441 | 0.005284 | 0.006247 | 0.005214 | 0.008535 | 0.005077 | 0.006174 | 0.009451 | 0.008523 |
Nucleolus | 0.001672 | 0.000505 | 0.000017 | 0.000026 | 0.000168 | 0.000213 | 0.000026 | 0.000085 | 0.000611 | 0.000345 | 0.000022 | 0.000077 |
Nucleus | 0.006655 | 0.012708 | 0.003269 | 0.000364 | 0.018670 | 0.015136 | 0.001856 | 0.000558 | 0.015131 | 0.014039 | 0.002545 | 0.000534 |
Peroxisomes | 0.004076 | 0.001485 | 0.000079 | 0.000115 | 0.001415 | 0.002092 | 0.000320 | 0.000564 | 0.002199 | 0.001818 | 0.000202 | 0.000508 |
Vacuole | 0.004992 | 0.013894 | 0.010996 | 0.027831 | 0.014884 | 0.019945 | 0.004468 | 0.018078 | 0.011970 | 0.017212 | 0.007653 | 0.019276 |
Vacuole Periphery | 0.001737 | 0.001907 | 0.000524 | 0.008854 | 0.000987 | 0.003269 | 0.000431 | 0.004440 | 0.001208 | 0.002654 | 0.000476 | 0.004983 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.30 | 0.57 | 1.31 | 0.24 | 1.09 | -2.77 | -3.06 | -5.00 | -4.60 | -3.85 | -0.13 | -1.79 | -4.30 | -4.36 | -3.57 |
Bud Neck | 0.97 | 0.07 | -0.50 | -1.69 | -0.65 | -2.04 | 0.82 | -3.82 | -1.67 | -4.78 | -1.09 | 0.27 | -3.74 | -2.95 | -4.21 |
Bud Site | 2.76 | 2.12 | 3.51 | 1.30 | 2.33 | -4.78 | -4.30 | -8.50 | -5.25 | -5.12 | -0.83 | -1.67 | -5.17 | -4.73 | -4.08 |
Cell Periphery | 4.46 | 3.93 | -5.76 | -7.00 | -6.87 | -2.68 | -7.58 | -12.50 | -12.11 | -9.86 | 3.44 | 0.20 | -11.49 | -13.39 | -12.11 |
Cytoplasm | -15.52 | -2.19 | 9.67 | 24.62 | 13.89 | 0.88 | 27.21 | 33.52 | 31.74 | 10.70 | -3.18 | 22.99 | 33.31 | 40.51 | 18.39 |
Cytoplasmic Foci | -1.38 | 5.63 | 5.96 | 13.31 | 2.51 | -3.89 | 15.28 | 12.50 | 15.17 | -5.27 | -3.37 | 15.31 | 12.77 | 18.42 | -4.96 |
Eisosomes | 0.72 | 2.89 | -1.40 | -1.75 | -2.56 | 0.53 | 4.70 | 1.43 | 1.12 | -3.25 | 0.78 | 5.48 | 0.50 | -0.17 | -4.24 |
Endoplasmic Reticulum | 8.27 | -2.29 | 1.29 | -4.54 | 2.98 | 2.48 | -26.48 | -10.73 | -12.90 | 14.74 | 4.65 | -22.40 | -7.56 | -12.48 | 12.71 |
Endosome | -1.34 | -2.05 | -3.67 | -3.20 | -2.79 | -6.29 | 0.31 | -16.29 | -11.88 | -16.23 | -5.96 | -2.25 | -16.43 | -13.39 | -15.38 |
Golgi | -3.47 | 5.40 | -2.33 | 0.91 | -7.49 | -2.50 | 9.44 | -6.20 | -4.36 | -10.91 | -3.76 | 11.35 | -6.79 | -4.54 | -11.77 |
Lipid Particles | -6.85 | -0.43 | -4.19 | -2.64 | -4.10 | -0.13 | 13.30 | -7.10 | -7.06 | -15.33 | -1.10 | 11.94 | -9.48 | -9.09 | -15.62 |
Mitochondria | -0.28 | 4.14 | 3.69 | 4.84 | -0.57 | -4.98 | 4.34 | 1.77 | 6.22 | -2.40 | -4.20 | 6.33 | 2.88 | 6.74 | -3.18 |
Mitotic Spindle | -1.82 | -1.21 | -1.55 | 0.26 | -1.27 | -2.55 | -0.92 | -3.48 | -2.69 | -3.39 | -2.55 | -1.94 | -3.62 | -1.83 | -3.35 |
None | 0.85 | 2.70 | 3.89 | 4.70 | 3.36 | -1.74 | 0.06 | 1.68 | 3.13 | 1.63 | -0.89 | 2.13 | 3.15 | 4.09 | 1.48 |
Nuclear Periphery | -4.26 | -5.27 | -6.87 | -3.10 | -3.87 | 4.04 | 6.95 | 6.53 | 3.43 | -1.72 | 3.26 | 5.58 | 5.24 | 2.75 | -1.07 |
Nuclear Periphery Foci | -1.37 | -7.25 | -1.56 | -1.05 | 2.08 | -1.04 | -0.11 | -3.97 | -2.51 | -3.39 | -1.60 | -6.08 | -4.45 | -3.09 | 0.82 |
Nucleolus | 1.53 | 2.23 | 2.22 | 2.75 | -0.96 | -0.95 | 3.54 | 0.94 | 1.83 | -1.54 | 1.13 | 2.66 | 2.29 | 2.71 | -1.64 |
Nucleus | -2.79 | 2.42 | 3.58 | 7.79 | 2.60 | 2.55 | 15.02 | 16.53 | 16.43 | 3.32 | 1.06 | 13.74 | 16.11 | 17.60 | 6.17 |
Peroxisomes | 2.49 | 3.91 | 3.87 | 8.15 | -0.66 | -3.05 | 8.42 | 4.32 | 6.28 | -1.44 | 1.11 | 6.35 | 4.95 | 7.04 | -2.14 |
Vacuole | -5.92 | -5.16 | -5.27 | -3.26 | -3.70 | -2.70 | 7.96 | -2.35 | 0.34 | -9.37 | -4.01 | 3.57 | -5.08 | -1.84 | -8.04 |
Vacuole Periphery | -0.32 | 2.79 | -1.83 | -1.79 | -2.15 | -4.04 | 3.19 | -6.68 | -1.64 | -7.53 | -4.10 | 4.31 | -5.69 | -3.26 | -6.84 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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