Standard name
Human Ortholog
Description Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0 0.05 0 0 0 0 0 0 0 0 0 – – – 0.05 0.09 0.05 0.1 0.11 0.09 0 0 0.06
Bud 0.06 0.06 0 0.06 0 0 0 0 0 0 0.07 0 – – – 0 0.05 0.05 0 0.11 0.06 0 0.15 0.09
Bud Neck 0 0 0.06 0 0 0 0 0 0 0.05 0 0 – – – 0 0.07 0 0.05 0.07 0.07 0.27 0 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 0.78 0.9 0.92 0.93 0.89 0.86 0.84 0.68 0.6 0.47 0.55 – – – 0.81 0.82 0.79 0.34 0.37 0.3 0.07 0.49 0.42
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – 0.08 0.05 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0 0 0 0.08 0.12 0.13 0.38 0.38 0.63 0.43 – – – 0 0 0.05 0.26 0.13 0.27 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.2 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0.05 0 0 – – – 0.05 0.05 0.06 0 0 0 0.22 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0.05 0.07 0.1 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 2 4 5 0 2 0 0 0 1 4 2 – – – 13 17 10 21 36 10 0 0 1
Bud 9 14 1 8 2 1 1 2 6 3 10 5 – – – 11 9 11 8 35 7 0 3 1
Bud Neck 0 3 5 3 5 4 6 6 1 8 2 3 – – – 2 13 4 10 23 8 1 0 1
Bud Site 1 0 1 0 0 0 0 0 2 1 0 0 – – – 3 2 2 – – – – – –
Cell Periphery 0 0 1 0 0 1 0 2 0 0 1 2 – – – 1 2 3 2 4 4 0 0 0
Cytoplasm 102 198 79 128 158 184 232 183 129 90 67 93 – – – 214 161 160 71 124 36 0 11 8
Endoplasmic Reticulum 7 5 0 0 0 1 0 0 3 2 6 4 – – – 22 10 21 4 6 1 0 0 0
Endosome 0 11 0 0 1 2 3 2 2 0 0 0 – – – 2 2 2 10 18 2 0 0 0
Golgi 0 1 0 0 2 1 0 0 0 0 0 0 – – – 1 0 0 0 1 0 0 0 0
Mitochondria 37 11 0 4 3 16 32 28 73 58 91 72 – – – 6 3 10 54 41 33 0 0 0
Nucleus 0 0 1 1 2 1 1 1 1 3 1 5 – – – 6 0 2 0 1 0 0 0 0
Nuclear Periphery 1 1 0 1 0 0 0 0 0 1 0 0 – – – 0 1 0 0 1 0 0 0 0
Nucleolus 0 1 0 0 0 1 0 0 0 1 0 0 – – – 0 0 1 0 0 0 0 0 0
Peroxisomes 1 2 0 0 0 0 0 0 1 0 0 0 – – – 2 2 1 2 2 0 0 0 0
SpindlePole 4 3 2 3 3 2 7 11 5 7 1 6 – – – 13 9 12 5 9 4 0 0 0
Vac/Vac Membrane 1 14 2 0 3 3 7 3 4 3 3 4 – – – 10 8 4 10 15 8 0 2 1
Unique Cell Count 153 254 88 139 169 207 270 217 191 151 144 169 265 196 203 212 333 124 4 24 21
Labelled Cell Count 174 266 96 153 179 219 289 238 227 178 186 196 306 239 243 212 333 124 4 24 21


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.6 5.3 4.9 5.3 4.3 4.3 4.7 3.7 3.9 3.3 3.6 – – – 5.6 5.6 5.2 – – –
Std Deviation (1e-4) 0.4 1.3 1.5 1.8 1.9 1.6 1.8 1.6 1.5 1.5 1.0 1.0 – – – 1.2 1.3 1.0 – – –
Intensity Change (Log2) – – – -0.11 0.0 -0.3 -0.29 -0.18 -0.49 -0.43 -0.68 -0.55 – – – 0.09 0.1 -0.02 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5905 -0.8516 -0.6908 -1.0271 -0.3591 -0.5542 -0.5699 -0.7956 -0.8742 -0.7025 -0.5207 -0.9299 0.0657 -0.7968 -0.3378 0.218 -1.0143 -0.4732
Actin 0.0222 0.0104 0.0134 0.0442 0.0085 0.0219 0.0279 0.0017 0.0093 0.0147 0.0094 0.0014 0.0521 0.0035 0.081 0.0143 0.003 0.0006
Bud 0.0044 0.0027 0.0285 0.0278 0.0149 0.0031 0.0013 0.001 0.0115 0.002 0.0007 0.0007 0.0051 0.0055 0.0252 0.0004 0.0001 0.0002
Bud Neck 0.008 0.0022 0.0019 0.0027 0.0028 0.1134 0.0082 0.0088 0.0034 0.0006 0.0009 0.1134 0.0046 0.0015 0.0028 0.0018 0 0.0613
Bud Periphery 0.0095 0.0064 0.1143 0.0404 0.0311 0.011 0.0056 0.0013 0.0537 0.0239 0.0013 0.0039 0.0079 0.0024 0.0279 0.0007 0.0002 0.0002
Bud Site 0.025 0.036 0.0148 0.0059 0.0043 0.0016 0.0068 0.0525 0.0138 0.0079 0.0028 0.0005 0.0515 0.0506 0.0382 0.0015 0 0.0004
Cell Periphery 0.0022 0.0008 0.0011 0.0009 0.0007 0.0005 0.0011 0.001 0.0042 0.0014 0.0003 0.001 0.003 0.0006 0.0009 0.0003 0 0.0005
Cytoplasm 0.0493 0.0486 0.0476 0.0628 0.1286 0.0754 0.0349 0.0372 0.0359 0.0346 0.0203 0.0731 0.102 0.092 0.1498 0.2168 0.0341 0.3764
Cytoplasmic Foci 0.0652 0.0484 0.0554 0.0158 0.0523 0.0498 0.0352 0.0264 0.032 0.0166 0.0154 0.0268 0.0706 0.0249 0.0383 0.0365 0.0042 0.0477
Eisosomes 0.0018 0.001 0.0009 0.0003 0.0002 0.0005 0.0006 0.0003 0.001 0.0015 0.0003 0.0002 0.0011 0.0003 0.0004 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0053 0.003 0.0021 0.0036 0.0075 0.0013 0.003 0.0002 0.0019 0.0015 0.0023 0.001 0.0039 0.0011 0.0034 0.0029 0.0009 0.0055
Endosome 0.0235 0.0051 0.0122 0.0168 0.1196 0.0047 0.0284 0.0001 0.006 0.0057 0.049 0.0012 0.0388 0.0028 0.0227 0.0443 0.0005 0.023
Golgi 0.005 0.0018 0.0064 0.0073 0.0372 0.006 0.0103 0.0002 0.0023 0.0018 0.0326 0.0003 0.024 0.005 0.0149 0.0158 0.0002 0.0022
Lipid Particles 0.0223 0.0027 0.0148 0.0094 0.0184 0.0119 0.0212 0.0002 0.0072 0.0043 0.0193 0.0018 0.0435 0.0015 0.0074 0.0628 0.0003 0.0036
Mitochondria 0.0132 0.0042 0.0263 0.0162 0.0153 0.0099 0.0244 0.0003 0.0043 0.0265 0.0902 0.0028 0.0094 0.0024 0.005 0.006 0.0002 0.0017
None 0.6753 0.8006 0.6036 0.6787 0.489 0.6649 0.7486 0.8638 0.7957 0.8458 0.7126 0.7629 0.5306 0.801 0.5316 0.5549 0.9544 0.4183
Nuclear Periphery 0.0148 0.0007 0.0025 0.012 0.0126 0.0026 0.0145 0 0.0004 0.0011 0.012 0.0011 0.0027 0.0003 0.0052 0.0027 0.0004 0.0011
Nucleolus 0.0078 0.0031 0.0021 0.0055 0.0132 0.0008 0.0021 0.0006 0.0009 0.0007 0.0005 0.0008 0.0027 0.0002 0.0006 0.0005 0 0.0002
Nucleus 0.0105 0.0015 0.0026 0.0124 0.0103 0.0021 0.003 0.0004 0.0007 0.001 0.0019 0.0017 0.0022 0.0009 0.002 0.0009 0.0002 0.0015
Peroxisomes 0.0119 0.0122 0.0318 0.0191 0.005 0.0049 0.0119 0.0024 0.0096 0.0038 0.0199 0.0012 0.0273 0.0018 0.0315 0.028 0.0003 0.048
Punctate Nuclear 0.0158 0.006 0.0146 0.0073 0.0108 0.0078 0.0044 0.0013 0.0018 0.0028 0.001 0.0025 0.0095 0.0008 0.0068 0.0033 0.0002 0.0048
Vacuole 0.0046 0.0022 0.0024 0.0077 0.0121 0.0032 0.0045 0.0003 0.0038 0.0013 0.0035 0.0013 0.0067 0.0008 0.0037 0.0042 0.0003 0.0023
Vacuole Periphery 0.0022 0.0003 0.0008 0.0032 0.0055 0.0028 0.0022 0 0.0005 0.0005 0.0037 0.0003 0.0011 0.0001 0.0009 0.0012 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.6565 20.6856 13.7339 12.282 8.29 7.3033 20.64 20.803 16.2885 11.2016
Translational Efficiency 0.3017 0.3014 0.4988 0.3612 0.3233 0.383 0.3269 0.4016 0.3669 0.3458

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1017 480 236 730 924 2332 89 1487 1941 2812 325 2217

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 570.17 705.91 939.26 811.41 680.75 689.72 916.31 800.76 622.81 692.48 932.98 804.27
Standard Deviation 65.32 101.36 103.14 96.94 73.42 89.48 90.05 100.29 88.61 91.82 100.25 99.33
Intensity Change Log 2 — 0.308092 0.720132 0.509039 — 0.018886 0.428711 0.234245 — 0.157927 0.568873 0.366014

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000346 0.001066 0.001303 0.002704 0.000195 0.000796 0.003872 0.002272 0.000274 0.000842 0.002006 0.002414
Bud Neck 0.057310 0.114608 0.023199 0.047968 0.033296 0.154317 0.028657 0.066462 0.045878 0.147539 0.024694 0.060372
Bud Site 0.012800 0.059675 0.034467 0.110497* 0.005945 0.064435 0.036544 0.122689* 0.009537 0.063622 0.035036 0.118675*
Cell Periphery 0.000816 0.000385 0.000364 0.000403 0.000231 0.000358 0.000749 0.000467 0.000538 0.000363 0.000469 0.000446
Cytoplasm 0.451160* 0.224741* 0.234297* 0.356025* 0.433323* 0.294248* 0.249856* 0.386353* 0.442669* 0.282383* 0.238557* 0.376367*
Cytoplasmic Foci 0.200967* 0.247975* 0.023497 0.041326 0.260870* 0.159865 0.077321 0.034162 0.229483* 0.174905 0.038236 0.036521
Eisosomes 0.000742 0.000278 0.000136 0.000179 0.000303 0.000239 0.000305 0.000152 0.000533 0.000246 0.000183 0.000161
Endoplasmic Reticulum 0.001322 0.001434 0.011766 0.002781 0.001001 0.000714 0.005051 0.001555 0.001170 0.000837 0.009927 0.001959
Endosome 0.006692 0.054051 0.008185 0.006050 0.012691 0.041273 0.011671 0.007806 0.009548 0.043454 0.009140 0.007228
Golgi 0.002273 0.007553 0.000169 0.001254 0.002442 0.006854 0.001721 0.003847 0.002353 0.006973 0.000594 0.002993
Lipid Particles 0.018543 0.009105 0.004875 0.002578 0.011968 0.006817 0.006260 0.001429 0.015413 0.007208 0.005254 0.001807
Mitochondria 0.009078 0.029126 0.000441 0.003211 0.006025 0.037915 0.000708 0.005103 0.007625 0.036415 0.000514 0.004480
Mitotic Spindle 0.000211 0.005862 0.000514 0.013467 0.000157 0.007263 0.007575 0.025729 0.000185 0.007024 0.002448 0.021691
None 0.010500 0.001193 0.001521 0.004572 0.002648 0.002411 0.002669 0.002187 0.006762 0.002203 0.001836 0.002973
Nuclear Periphery 0.000508 0.000352 0.002378 0.001084 0.000602 0.000330 0.001270 0.000863 0.000553 0.000333 0.002074 0.000935
Nuclear Periphery Foci 0.000237 0.000541 0.005264 0.001868 0.000285 0.000260 0.002382 0.001103 0.000260 0.000308 0.004475 0.001355
Nucleolus 0.001777 0.002385 0.002211 0.000565 0.000975 0.002624 0.001732 0.000625 0.001395 0.002583 0.002080 0.000605
Nucleus 0.184613* 0.080380 0.455623* 0.170090* 0.169487* 0.074579 0.380034* 0.143500* 0.177412* 0.075569 0.434923* 0.152255*
Peroxisomes 0.009891 0.047513 0.002082 0.009780 0.011131 0.023598 0.010114 0.009308 0.010482 0.027680 0.004281 0.009463
Vacuole 0.028883 0.106574 0.187422 0.222535* 0.045433 0.115780 0.170993 0.181864 0.036761 0.114208 0.182923 0.195256
Vacuole Periphery 0.001332 0.005203 0.000287 0.001065 0.000991 0.005325 0.000517 0.002525 0.001170 0.005304 0.000350 0.002045

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.59 -3.31 -5.65 -3.24 -1.05 -5.70 -2.30 -8.43 -4.33 1.20 -4.81 -3.90 -9.22 -5.55 0.55
Bud Neck -5.70 5.38 4.66 8.95 -1.75 -22.19 1.31 -2.48 20.31 -2.55 -20.69 4.86 1.63 23.28 -3.90
Bud Site -8.73 -2.04 -8.58 -0.97 -4.28 -22.31* -2.97 -16.17 -2.90 -4.97 -22.02 -3.37 -17.60 -2.73 -7.08
Cell Periphery 3.41 3.55 4.23 1.76 2.08 -4.44 -3.30 -1.98 1.33 2.73 2.63 0.94 3.44 1.94 3.18
Cytoplasm 17.41 15.90 11.58 -5.44 -5.78 14.01 8.49 8.36 -5.00 -4.67 21.42 18.00 13.90 -5.92 -8.91
Cytoplasmic Foci -4.17 28.31 26.68 21.56 -3.70 12.95 15.78 33.75 33.95 4.13 9.42 34.31 42.99 39.23 1.72
Eisosomes 3.97 5.04 4.96 5.93 -0.69 1.54 -0.34 4.16 9.00 3.58 4.46 5.00 6.08 10.05 3.29
Endoplasmic Reticulum -0.23 -11.62 -5.08 -6.63 9.85 1.82 -5.25 -7.54 -13.03 3.86 1.41 -12.78 -6.66 -15.38 11.09
Endosome -9.58 -2.05 2.83 10.02 4.00 -14.77 0.85 6.26 22.14 3.44 -20.37 0.13 6.63 24.23 5.16
Golgi -5.52 7.35 4.10 7.28 -1.51 -6.52 1.42 0.73 6.31 -0.78 -8.77 5.59 1.83 8.95 -2.23
Lipid Particles 4.83 7.85 9.98 5.45 4.69 4.71 4.70 10.19 12.46 7.01 7.93 9.23 13.99 12.89 8.41
Mitochondria -5.30 7.99 5.22 7.43 -1.23 -13.26 4.03 2.02 16.56 -3.31 -14.66 8.33 5.01 18.32 -3.52
Mitotic Spindle -2.51 -2.05 -4.28 -1.79 -3.88 -7.45 -1.16 -7.96 -4.57 -3.17 -7.77 -1.75 -9.02 -4.76 -6.82
None 5.57 5.47 3.70 -1.74 -1.58 0.54 0.89 1.00 0.61 -0.33 4.93 5.67 4.37 -0.70 -2.23
Nuclear Periphery 2.00 -11.90 -7.88 -8.59 5.46 2.66 -5.07 -5.69 -9.52 0.85 3.38 -12.27 -9.69 -12.55 6.50
Nuclear Periphery Foci -1.94 -8.32 -6.27 -4.68 5.68 0.38 -5.45 -4.65 -4.58 3.90 -0.77 -9.40 -7.42 -6.98 7.27
Nucleolus -1.10 -0.09 3.51 3.78 1.09 -6.09 -0.96 3.06 7.48 1.90 -4.18 -0.38 4.19 8.41 1.27
Nucleus 12.14 -12.84 -1.28 -11.80 11.82 15.57 -5.63 -2.17 -18.81 5.09 21.45 -14.10 -2.07 -22.82 13.27
Peroxisomes -7.43 6.47 2.65 8.26 -3.54 -6.76 0.62 3.55 12.47 1.11 -10.77 5.13 4.51 15.03 -1.59
Vacuole -11.74 -14.40 -27.33 -17.02 -4.66 -17.39 -7.83 -31.30 -20.94 -0.84 -23.87 -16.07 -42.22 -27.14 -3.38
Vacuole Periphery -5.87 5.73 2.18 6.57 -0.72 -13.63 3.80 -0.59 6.99 -1.67 -14.14 7.53 0.36 9.65 -2.01
Standard name
Human Ortholog
Description Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Bud8

Bud8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available