Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.14 0.11 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.91 0.99 0.93 0.93 0.86 0.89 0.8 0.67 0.83 0.64 0.99 0.99 0.99 0.94 0.92 0.96 0.78 0.78 0.67 0.59 0.66 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.1 0 0.08 0.12 0.19 0.23 0.35 0.38 0.32 0.58 0 0 0 0 0 0 0 0.05 0.19 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 18 15 4 11 17 9
Bud 0 4 1 0 0 0 2 1 2 1 3 1 0 0 3 0 1 0 5 8 0 4 7 10
Bud Neck 2 5 0 0 0 0 1 3 1 0 0 0 0 0 0 2 3 0 15 9 2 5 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 4 0 1 3 2 2 2 3 3 1 1 1 8 14 13 6 7 7 1 1 0 3
Cytoplasm 380 450 70 88 66 87 84 151 78 37 52 29 148 168 178 396 205 263 310 360 69 47 102 147
Endoplasmic Reticulum 4 2 1 0 2 1 0 0 0 0 0 0 0 1 0 35 12 23 5 1 0 0 2 1
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 5 4 3 5 0 1 2 2
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 2 2 1 0 0 1 0
Mitochondria 1 15 8 4 6 11 19 39 34 21 20 26 1 0 3 18 2 3 13 22 19 4 7 9
Nucleus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 0
SpindlePole 4 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 3 1 4 7 0 0 5 7
Vac/Vac Membrane 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 6 6 4 4 9 0 1 1 4
Unique Cell Count 393 468 77 89 71 94 98 169 97 55 63 45 149 169 179 420 222 274 399 464 104 82 156 208
Labelled Cell Count 403 483 84 92 76 102 109 196 118 63 79 57 150 170 192 484 256 310 399 464 104 82 156 208


Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.8 2.9 3.1 3.2 3.1 2.9 3.0 2.6 2.9 3.0 2.7 4.4 4.9 4.2 6.5 7.3 7.0 4.0 4.8 5.2
Std Deviation (1e-4) 0.9 0.7 0.2 0.8 0.3 1.1 1.0 0.7 0.7 1.5 1.2 0.7 1.5 1.6 1.6 2.5 3.3 3.0 0.9 1.9 1.9
Intensity Change (Log2) – – – 0.1 0.16 0.08 -0.0 0.03 -0.16 -0.02 0.03 -0.09 0.6 0.75 0.54 1.17 1.34 1.28 0.45 0.72 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5626 1.4954 1.3747 1.2735 0.843 1.315 4.9959 3.5988 3.8982 3.8325 3.7947 3.9033 3.6126 3.8715 3.9026 4.3377 3.375 3.7312
Actin 0.0083 0.0014 0.0143 0.0017 0.0016 0.0015 0.1162 0.0028 0.0365 0.0349 0.0054 0.01 0.0166 0.0262 0.0293 0.0016 0.0035 0.0042
Bud 0.0005 0.0007 0.0138 0.0005 0.0002 0.0002 0.0044 0.0063 0.0172 0.0012 0.0011 0.007 0.0024 0.0137 0.0197 0.0001 0.0055 0.0002
Bud Neck 0.0264 0.001 0.0007 0.0017 0.0016 0.2651 0.0247 0.0041 0.0017 0.0584 0.0005 0.1274 0.0169 0.0037 0.0025 0.0002 0.0172 0.1528
Bud Periphery 0.0013 0.0015 0.0387 0.001 0.0005 0.0005 0.0143 0.0112 0.0629 0.0041 0.0022 0.0124 0.0142 0.0088 0.0287 0.0003 0.0142 0.0003
Bud Site 0.0339 0.0421 0.0147 0.0003 0.0002 0.0042 0.0344 0.0519 0.0108 0.0011 0.0007 0.0013 0.0483 0.0964 0.0091 0.0001 0.0072 0.0006
Cell Periphery 0.0058 0.0044 0.0027 0.0005 0.0006 0.0012 0.0033 0.0013 0.0014 0.0014 0.0004 0.0009 0.0086 0.0014 0.0009 0.0005 0.002 0.0006
Cytoplasm 0.393 0.5155 0.4927 0.4626 0.7597 0.5176 0.1064 0.2829 0.2609 0.2552 0.2448 0.3346 0.4421 0.3469 0.3249 0.2463 0.3581 0.3722
Cytoplasmic Foci 0.0087 0.0075 0.018 0.0069 0.0043 0.0094 0.043 0.0123 0.0159 0.032 0.0145 0.0125 0.0058 0.0149 0.0246 0.0052 0.0704 0.0112
Eisosomes 0.0011 0.0007 0.0006 0.0007 0.0001 0.0001 0.0022 0.0002 0.0005 0.0005 0.0002 0.0002 0.0007 0.0003 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0035 0.004 0.0061 0.0048 0.0038 0.0013 0.0078 0.0021 0.0044 0.0021 0.0046 0.0015 0.0024 0.0009 0.0021 0.0038 0.0019 0.0019
Endosome 0.0007 0.0013 0.0037 0.0034 0.0014 0.0009 0.0248 0.0019 0.0151 0.0029 0.048 0.0111 0.0101 0.0013 0.0025 0.0017 0.002 0.0005
Golgi 0.0004 0.0004 0.0019 0.0003 0.0002 0.0003 0.0136 0.0005 0.007 0.0019 0.0152 0.0037 0.0015 0.0006 0.0022 0.0006 0.0003 0.0001
Lipid Particles 0.0006 0.0005 0.0009 0.0006 0.0001 0.0001 0.0297 0.0004 0.008 0.0007 0.0013 0.0005 0.0004 0.0006 0.0012 0.0005 0.0001 0.0001
Mitochondria 0.0002 0.0004 0.0026 0.0005 0.0002 0.0002 0.0215 0.0006 0.0068 0.0011 0.0278 0.0019 0.0012 0.0004 0.0157 0.0003 0.0003 0.0001
None 0.5118 0.4147 0.3808 0.5078 0.2227 0.1958 0.4354 0.6139 0.5304 0.5955 0.6224 0.4656 0.4131 0.4786 0.5207 0.7363 0.5139 0.4538
Nuclear Periphery 0.0003 0.0004 0.0008 0.001 0.0003 0.0001 0.0277 0.0004 0.0025 0.0014 0.0032 0.0013 0.0019 0.0004 0.0011 0.0005 0.0002 0.0001
Nucleolus 0.0005 0.0002 0.0002 0.0006 0.0001 0.0001 0.0031 0.0008 0.0003 0.0003 0.0001 0.0003 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001
Nucleus 0.0011 0.001 0.0012 0.0018 0.0009 0.0005 0.0119 0.0013 0.0017 0.0005 0.001 0.0036 0.0035 0.0016 0.0016 0.0004 0.0004 0.0003
Peroxisomes 0.0003 0.0001 0.0028 0.0002 0.0001 0.0001 0.0506 0.001 0.01 0.0012 0.0006 0.0013 0.0002 0.0008 0.0102 0.0001 0.001 0.0001
Punctate Nuclear 0.0009 0.0006 0.0008 0.002 0.0003 0.0003 0.0136 0.0016 0.0013 0.0007 0.0008 0.0016 0.0016 0.0015 0.0011 0.0003 0.0009 0.0003
Vacuole 0.0007 0.0014 0.0016 0.0011 0.0009 0.0004 0.0089 0.0023 0.0039 0.0024 0.003 0.0009 0.0072 0.0007 0.001 0.0006 0.0006 0.0002
Vacuole Periphery 0 0.0001 0.0002 0.0001 0.0001 0 0.0026 0.0001 0.0008 0.0004 0.0023 0.0003 0.0009 0.0001 0.0003 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.5663 13.5684 13.1248 20.5397 24.4234 19.775 19.1086 21.2471 20.6712 24.9434
Translational Efficiency 0.4657 0.3566 0.4358 0.2611 0.2544 0.4696 0.3717 0.3235 0.3576 0.3379

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
143 1540 137 790 1641 2008 111 764 1784 3548 248 1554

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 578.43 695.83 954.90 802.02 650.06 722.67 922.39 772.53 644.32 711.02 940.35 787.52
Standard Deviation 62.71 92.84 103.24 96.45 77.43 99.53 110.46 92.05 78.79 97.59 107.75 95.46
Intensity Change Log 2 — 0.266593 0.723207 0.471496 — 0.152764 0.504804 0.249018 — 0.207480 0.611765 0.358053

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000074 0.000805 0.000852 0.000742 0.000180 0.000664 0.001169 0.000489 0.000172 0.000725 0.000994 0.000618
Bud Neck 0.019607 0.045482 0.003273 0.008217 0.020003 0.065689 0.006516 0.012500 0.019972 0.056918 0.004725 0.010323
Bud Site 0.002335 0.019967 0.008348 0.050215 0.005909 0.043948 0.003306 0.036470 0.005623 0.033539 0.006091 0.043457
Cell Periphery 0.000187 0.000174 0.000473 0.000243 0.000147 0.000242 0.000964 0.000234 0.000150 0.000213 0.000693 0.000238
Cytoplasm 0.465157* 0.409771* 0.207440* 0.466607* 0.521313* 0.404439* 0.172626* 0.481071* 0.516811* 0.406754* 0.191858* 0.473718*
Cytoplasmic Foci 0.284860* 0.271152* 0.024700 0.027106 0.192541* 0.222776* 0.031673 0.043526 0.199941* 0.243774* 0.027821 0.035179
Eisosomes 0.000320 0.000228 0.000191 0.000073 0.000198 0.000234 0.000171 0.000104 0.000208 0.000231 0.000182 0.000088
Endoplasmic Reticulum 0.000279 0.000574 0.010409 0.002421 0.000709 0.000899 0.006423 0.001301 0.000674 0.000758 0.008625 0.001871
Endosome 0.015423 0.030170 0.009000 0.005714 0.008489 0.032601 0.010862 0.006690 0.009044 0.031546 0.009833 0.006194
Golgi 0.000607 0.007637 0.000179 0.001290 0.002153 0.010233 0.003787 0.001559 0.002029 0.009106 0.001794 0.001422
Lipid Particles 0.008340 0.006708 0.007585 0.001721 0.006751 0.006199 0.005132 0.001582 0.006878 0.006420 0.006487 0.001653
Mitochondria 0.000796 0.009689 0.000419 0.006306 0.003657 0.018105 0.006755 0.007298 0.003428 0.014452 0.003255 0.006793
Mitotic Spindle 0.000016 0.002210 0.000306 0.032008 0.001373 0.016054 0.000121 0.017323 0.001265 0.010045 0.000223 0.024789
None 0.000805 0.003377 0.001758 0.003138 0.002744 0.002112 0.003153 0.002374 0.002588 0.002661 0.002383 0.002762
Nuclear Periphery 0.000278 0.000234 0.002567 0.001413 0.000333 0.000628 0.001888 0.000738 0.000328 0.000457 0.002263 0.001081
Nuclear Periphery Foci 0.000214 0.000399 0.004157 0.001336 0.000344 0.000413 0.002030 0.000630 0.000333 0.000407 0.003205 0.000989
Nucleolus 0.001823 0.001376 0.000925 0.000248 0.000792 0.001244 0.001103 0.000331 0.000875 0.001302 0.001005 0.000289
Nucleus 0.124233 0.074305 0.523408* 0.199003* 0.178664* 0.064198 0.600834* 0.178493* 0.174301* 0.068585 0.558062* 0.188919*
Peroxisomes 0.004785 0.022482 0.000871 0.001814 0.004713 0.017013 0.000618 0.001215 0.004719 0.019387 0.000758 0.001520
Vacuole 0.069376 0.090923 0.192822 0.187770 0.048427 0.088073 0.139786 0.204876* 0.050106 0.089310 0.169084 0.196180
Vacuole Periphery 0.000486 0.002337 0.000317 0.002614 0.000559 0.004235 0.001084 0.001198 0.000554 0.003411 0.000660 0.001918

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.43 -7.80 -8.35 2.33 3.70 -7.16 -2.02 -4.56 2.40 1.23 -9.51 -4.63 -7.75 3.28 2.68
Bud Neck -4.31 2.90 2.28 13.78 -3.50 -14.13 2.72 5.12 17.92 -0.30 -14.58 5.69 6.86 22.94 -1.13
Bud Site -12.81 -1.60 -6.41 -0.64 -2.47 -15.23 2.31 -4.94 5.16 -5.68 -17.06 -0.33 -7.09 4.16 -4.87
Cell Periphery 0.72 -4.88 0.37 -0.40 5.14 -6.31 -1.36 -3.38 3.08 1.21 -5.39 -2.54 -3.12 2.20 2.29
Cytoplasm 2.78 12.83 5.97 5.84 -10.30 12.74 21.22 9.74 0.38 -12.83 14.09 29.30 15.60 4.38 -16.45
Cytoplasmic Foci 0.82 16.10 16.41 42.15 0.97 -4.42 27.08 30.38 36.52 -1.94 -7.29 34.51 36.42 55.70 -1.23
Eisosomes 2.67 2.90 6.98 11.19 7.92 -1.92 0.92 6.93 9.09 4.38 -1.47 0.60 9.22 14.04 8.77
Endoplasmic Reticulum -3.26 -11.78 -15.57 -14.74 8.66 -0.98 -5.85 -8.09 -6.24 4.95 -0.61 -11.85 -13.40 -14.27 9.50
Endosome -4.36 1.99 4.25 14.95 6.10 -14.22 0.22 3.91 16.91 1.43 -16.45 0.25 6.32 22.83 4.05
Golgi -8.95 2.84 0.80 9.04 -0.99 -10.42 0.31 2.67 11.03 0.54 -12.12 1.59 4.25 14.53 0.35
Lipid Particles 1.27 0.62 5.14 10.00 7.29 0.79 2.21 8.93 9.23 5.33 0.80 0.74 9.80 13.42 8.92
Mitochondria -7.94 1.97 -1.46 5.42 -1.95 -9.04 0.21 -0.01 6.57 -0.19 -9.76 1.32 0.51 8.72 -0.82
Mitotic Spindle -3.98 -1.80 -5.99 -5.20 -5.61 -6.96 1.85 -3.76 0.66 -4.21 -6.72 0.99 -6.46 -2.39 -6.92
None -3.49 -2.45 -6.15 -0.56 -4.52 1.09 0.99 0.68 -0.45 -0.44 -0.07 1.69 -0.85 -0.94 -3.51
Nuclear Periphery 0.88 -17.71 -16.18 -16.90 2.35 -0.86 -11.39 -10.25 -1.79 5.72 -0.66 -20.04 -18.20 -6.78 5.82
Nuclear Periphery Foci -1.68 -8.79 -7.51 -5.29 6.56 -0.48 -4.78 -4.33 -3.44 3.87 -0.70 -9.47 -6.76 -6.33 7.55
Nucleolus 0.48 0.88 1.18 5.91 4.70 -1.30 -0.12 2.85 5.26 3.31 -1.16 0.73 3.16 7.89 5.30
Nucleus 4.86 -13.66 -10.17 -21.05 8.86 23.42 -12.94 -3.80 -18.64 11.50 23.85 -17.80 -8.54 -28.17 14.36
Peroxisomes -9.26 3.47 3.52 14.18 0.40 -11.06 9.45 8.42 15.82 -1.97 -15.04 9.64 9.20 21.05 -0.63
Vacuole -2.86 -9.39 -19.47 -20.98 -1.77 -11.60 -7.84 -23.87 -19.48 -2.59 -14.15 -13.20 -34.34 -28.66 -2.93
Vacuole Periphery -6.34 1.79 -0.67 3.23 -1.11 -8.15 -0.03 -0.39 7.35 -0.16 -10.01 0.70 -0.64 7.53 -0.96
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (25%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rom2

Rom2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rom2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available