Standard name | |
---|---|
Human Ortholog | |
Description | Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 3 | 5 | 14 | 21 | 24 | 33 | 51 | 58 | 49 | 48 | 5 | 12 | 21 | 3 | 4 | 0 | 2 | 3 | 2 | 1 | 2 | 3 |
Bud | 0 | 0 | 0 | 0 | 5 | 11 | 2 | 8 | 4 | 8 | 12 | 7 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 2 | 11 | 8 | 21 | 17 | 18 | 30 | 32 | 20 | 27 | 6 | 36 | 84 | 3 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 11 | 6 | 13 | 13 | 34 | 34 | 58 | 35 | 58 | 49 | 91 | 16 | 16 | 19 | 5 | 4 | 6 | 2 | 4 | 0 | 2 | 4 | 4 |
Endoplasmic Reticulum | 150 | 257 | 243 | 326 | 462 | 558 | 783 | 733 | 574 | 540 | 332 | 448 | 287 | 625 | 659 | 0 | 1 | 0 | 178 | 301 | 267 | 280 | 320 | 367 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 4 | 3 | 14 | 66 | 44 | 16 | 26 | 45 | 23 | 81 | 18 | 20 | 3 | 26 | 17 | 3 | 6 | 6 | 0 | 2 | 1 | 6 | 3 | 7 |
Mitochondria | 0 | 1 | 1 | 1 | 2 | 9 | 29 | 15 | 35 | 18 | 48 | 61 | 0 | 0 | 0 | 4 | 4 | 2 | 1 | 0 | 1 | 1 | 2 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 0 | 21 | 10 | 10 | 19 | 79 | 36 | 34 | 51 | 30 | 26 | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 2 | 5 | 5 | 5 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 2 | 8 | 13 | 3 | 9 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 3 | 4 | 7 | 44 | 37 | 76 | 69 | 30 | 94 | 35 | 47 | 1 | 2 | 6 | 3 | 4 | 2 | 2 | 3 | 0 | 2 | 3 | 13 |
Unique Cell Count | 165 | 273 | 256 | 369 | 539 | 637 | 913 | 907 | 694 | 770 | 480 | 660 | 300 | 656 | 726 | 17 | 20 | 19 | 191 | 322 | 280 | 303 | 353 | 421 |
Labelled Cell Count | 170 | 279 | 294 | 439 | 603 | 728 | 1080 | 1029 | 822 | 952 | 598 | 783 | 319 | 720 | 809 | 21 | 25 | 21 | 191 | 322 | 280 | 303 | 353 | 421 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 65.4 | 81.0 | 48.2 | 52.0 | 41.6 | 33.9 | 30.1 | 31.0 | 29.4 | 27.9 | 26.0 | 23.2 | 61.1 | 62.7 | 58.1 | 55.1 | 57.8 | 60.7 | 69.8 | 67.8 | 66.9 |
Std Deviation (1e-4) | 15.3 | 17.3 | 11.6 | 11.4 | 8.8 | 7.4 | 6.7 | 7.1 | 6.6 | 6.9 | 6.6 | 7.0 | 13.6 | 11.9 | 11.8 | 16.9 | 21.7 | 19.8 | 14.5 | 16.9 | 17.2 |
Intensity Change (Log2) | – | – | – | 0.11 | -0.21 | -0.51 | -0.68 | -0.64 | -0.71 | -0.79 | -0.89 | -1.06 | 0.34 | 0.38 | 0.27 | 0.2 | 0.26 | 0.33 | 0.53 | 0.49 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 1.3 | 1.8 | 1.4 | 2.0 | 3.6 | 3.7 | 4.6 | 3.6 | 0 | 0 | 1.5 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.9 | 0 | 2.1 | 0 | 0 | 2.7 | 2.6 | 2.6 | 2.6 | 0 | 3.2 | 5.3 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 0.1 | 2.0 | 1.1 | 2.5 | 1.8 | 3.0 | 3.9 | 5.1 | 1.8 | 0.1 | 0.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | -2.8 | -3.8 | -3.3 | -4.0 | -5.4 | -4.8 | -8.1 | -8.1 | -8.5 | 0.4 | 0.2 | -2.1 | -12.1 | -12.1 | -12.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 4.6 | 1.4 | -2.2 | -2.0 | -0.3 | -1.5 | 2.4 | -1.1 | -1.8 | -3.1 | -1.0 | -2.5 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.5 | 0 | 3.3 | 0 | 5.0 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -3.1 | -4.3 | -3.4 | 0.2 | -2.8 | -1.9 | -0.9 | -1.0 | -2.6 | -4.7 | -7.1 | -7.2 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.7 | 2.7 | 3.8 | 3.5 | 2.0 | 5.0 | 3.3 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 65.9688 | 70.141 | 71.2184 | 67.3158 | 55.7033 | 66.3363 | 66.3676 | 70.8526 | 72.3344 | 69.8926 | 68.5291 | 67.8082 | 79.3259 | 77.6529 | 83.3232 | 78.9075 | 80.851 | 75.9368 |
Actin | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0028 | 0.0001 | 0.0071 | 0.0001 | 0.0006 | 0.001 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0002 | 0.0005 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0021 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0007 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Bud Site | 0.0005 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0191 | 0.0227 | 0.0362 | 0.0265 | 0.0369 | 0.0081 | 0.0138 | 0.0198 | 0.0208 | 0.0209 | 0.0182 | 0.002 | 0.0298 | 0.0426 | 0.0429 | 0.0232 | 0.0075 | 0.0082 |
Cytoplasm | 0.0035 | 0.0013 | 0.0009 | 0.0009 | 0.0037 | 0.0014 | 0.0018 | 0.003 | 0.0009 | 0.0002 | 0.001 | 0.0002 | 0.0012 | 0.0102 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
Cytoplasmic Foci | 0.0077 | 0 | 0.0057 | 0.0001 | 0.0092 | 0.0016 | 0.0223 | 0.0001 | 0.005 | 0.0039 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0012 | 0 | 0.0018 | 0.0004 | 0.0032 | 0.0001 | 0.001 | 0.0012 | 0.0013 | 0.001 | 0.0163 | 0.0001 | 0.0021 | 0.0015 | 0.0017 | 0.0005 | 0.0006 | 0.0003 |
Endoplasmic Reticulum | 0.754 | 0.9245 | 0.8828 | 0.8918 | 0.7506 | 0.8612 | 0.6296 | 0.8848 | 0.8902 | 0.8614 | 0.8542 | 0.7707 | 0.5768 | 0.8215 | 0.8471 | 0.9302 | 0.8067 | 0.7617 |
Endosome | 0.0011 | 0 | 0.0005 | 0.0008 | 0.0011 | 0.0004 | 0.0023 | 0.0001 | 0.0014 | 0.0008 | 0.0017 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0002 |
Golgi | 0.0005 | 0.0001 | 0.0009 | 0.0008 | 0.0014 | 0.0003 | 0.0025 | 0.0006 | 0.0012 | 0.0017 | 0.0035 | 0.0008 | 0.0005 | 0.0004 | 0.0009 | 0.0006 | 0.0051 | 0.0011 |
Lipid Particles | 0.0051 | 0.005 | 0.0058 | 0.0044 | 0.0116 | 0.0029 | 0.0164 | 0.0141 | 0.0013 | 0.0129 | 0.0092 | 0.0007 | 0.0012 | 0.0029 | 0.0013 | 0.0009 | 0.0011 | 0.0012 |
Mitochondria | 0.0024 | 0 | 0.0023 | 0.0002 | 0.0341 | 0.0016 | 0.0211 | 0.0001 | 0.0026 | 0.0002 | 0.0017 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 |
None | 0.003 | 0 | 0.0009 | 0.0003 | 0.0031 | 0.0017 | 0.0053 | 0.0001 | 0.0049 | 0.0001 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.1684 | 0.039 | 0.047 | 0.0607 | 0.0641 | 0.1029 | 0.2338 | 0.0675 | 0.0604 | 0.0706 | 0.0719 | 0.2158 | 0.3656 | 0.1048 | 0.0947 | 0.0403 | 0.169 | 0.2173 |
Nucleolus | 0.0036 | 0 | 0.0017 | 0.0001 | 0.0151 | 0.0018 | 0.0033 | 0 | 0.0005 | 0 | 0.0015 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0039 | 0.0002 | 0.0004 | 0.0008 | 0.0023 | 0.0024 | 0.0039 | 0.0006 | 0.0004 | 0.0004 | 0.0016 | 0.001 | 0.0028 | 0.0006 | 0.0003 | 0.0001 | 0.0009 | 0.001 |
Peroxisomes | 0.0002 | 0 | 0.002 | 0 | 0.0274 | 0 | 0.0015 | 0 | 0.0003 | 0.0193 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0024 | 0 | 0.0002 | 0 | 0.0004 | 0.0011 | 0.0037 | 0 | 0.0006 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0151 | 0.0066 | 0.0064 | 0.0073 | 0.019 | 0.0065 | 0.0125 | 0.0055 | 0.005 | 0.0029 | 0.0037 | 0.002 | 0.0091 | 0.0123 | 0.0064 | 0.0028 | 0.0017 | 0.0026 |
Vacuole Periphery | 0.0072 | 0.0004 | 0.0038 | 0.0043 | 0.0103 | 0.0054 | 0.017 | 0.0022 | 0.0022 | 0.0023 | 0.0119 | 0.0058 | 0.0092 | 0.0022 | 0.0025 | 0.0008 | 0.0058 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 314.651 | 206.2077 | 151.6048 | 179.2096 | 239.1245 | 182.7365 | 209.4572 | 141.3695 | 112.3831 | 261.9466 |
Translational Efficiency | 1.0729 | 1.3953 | 1.176 | 0.8363 | 1.0278 | 1.9179 | 1.1111 | 0.957 | 1.2021 | 1.0436 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
449 | 1460 | 84 | 805 | 1645 | 1907 | 813 | 1758 | 2094 | 3367 | 897 | 2563 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3543.97 | 4408.99 | 5628.71 | 5178.34 | 3662.32 | 4132.83 | 5605.73 | 5384.70 | 3636.94 | 4252.58 | 5607.88 | 5319.89 |
Standard Deviation | 661.13 | 1049.83 | 945.49 | 1051.78 | 893.39 | 886.15 | 899.69 | 1296.12 | 850.34 | 970.25 | 904.10 | 1228.38 |
Intensity Change Log 2 | — | 0.315082 | 0.667438 | 0.547123 | — | 0.174372 | 0.614144 | 0.556108 | — | 0.245287 | 0.640600 | 0.551696 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000715 | 0.000735 | 0.000610 | 0.000398 | 0.000590 | 0.001417 | 0.000139 | 0.001015 | 0.000617 | 0.001121 | 0.000183 | 0.000821 |
Bud Neck | 0.003341 | 0.001254 | 0.029181 | 0.004196 | 0.001237 | 0.000683 | 0.001371 | 0.002090 | 0.001688 | 0.000930 | 0.003976 | 0.002751 |
Bud Site | 0.000862 | 0.000500 | 0.001465 | 0.002086 | 0.000433 | 0.000274 | 0.000134 | 0.002306 | 0.000525 | 0.000372 | 0.000258 | 0.002237 |
Cell Periphery | 0.003090 | 0.003059 | 0.014643 | 0.017690 | 0.003387 | 0.002293 | 0.005236 | 0.010579 | 0.003323 | 0.002625 | 0.006117 | 0.012813 |
Cytoplasm | 0.000076 | 0.000183 | 0.000079 | 0.000497 | 0.000290 | 0.000295 | 0.000073 | 0.000647 | 0.000244 | 0.000246 | 0.000073 | 0.000600 |
Cytoplasmic Foci | 0.000325 | 0.000201 | 0.000235 | 0.000243 | 0.000342 | 0.000145 | 0.000081 | 0.000313 | 0.000338 | 0.000170 | 0.000096 | 0.000291 |
Eisosomes | 0.001714 | 0.001407 | 0.002585 | 0.001268 | 0.000814 | 0.001060 | 0.000952 | 0.000551 | 0.001007 | 0.001211 | 0.001105 | 0.000776 |
Endoplasmic Reticulum | 0.939380* | 0.942534* | 0.611507* | 0.877291* | 0.920062* | 0.963381* | 0.934163* | 0.913996* | 0.924204* | 0.954341* | 0.903948* | 0.902468* |
Endosome | 0.004588 | 0.004395 | 0.010374 | 0.006611 | 0.005369 | 0.002942 | 0.002359 | 0.006013 | 0.005202 | 0.003572 | 0.003109 | 0.006201 |
Golgi | 0.004178 | 0.005437 | 0.006518 | 0.012454 | 0.011920 | 0.003890 | 0.004589 | 0.009693 | 0.010260 | 0.004561 | 0.004769 | 0.010561 |
Lipid Particles | 0.005334 | 0.005839 | 0.028922 | 0.014029 | 0.013515 | 0.003665 | 0.002066 | 0.008570 | 0.011760 | 0.004608 | 0.004581 | 0.010285 |
Mitochondria | 0.001678 | 0.001731 | 0.003329 | 0.000729 | 0.001843 | 0.001162 | 0.000618 | 0.002346 | 0.001808 | 0.001409 | 0.000872 | 0.001838 |
Mitotic Spindle | 0.000691 | 0.000029 | 0.000372 | 0.000239 | 0.000037 | 0.000023 | 0.000028 | 0.000106 | 0.000178 | 0.000026 | 0.000060 | 0.000148 |
None | 0.000814 | 0.001526 | 0.000043 | 0.000317 | 0.001010 | 0.001473 | 0.000099 | 0.001578 | 0.000968 | 0.001496 | 0.000094 | 0.001182 |
Nuclear Periphery | 0.019711 | 0.013512 | 0.190042* | 0.020917 | 0.014554 | 0.009082 | 0.029210 | 0.012982 | 0.015660 | 0.011003 | 0.044271 | 0.015474 |
Nuclear Periphery Foci | 0.003631 | 0.004154 | 0.029367 | 0.008716 | 0.005053 | 0.001454 | 0.002926 | 0.005288 | 0.004748 | 0.002624 | 0.005402 | 0.006365 |
Nucleolus | 0.000113 | 0.000116 | 0.000215 | 0.000038 | 0.000070 | 0.000047 | 0.000032 | 0.000085 | 0.000079 | 0.000077 | 0.000049 | 0.000070 |
Nucleus | 0.000272 | 0.000219 | 0.000424 | 0.000195 | 0.000178 | 0.000184 | 0.000062 | 0.000320 | 0.000198 | 0.000199 | 0.000096 | 0.000281 |
Peroxisomes | 0.000041 | 0.000036 | 0.000022 | 0.000011 | 0.000041 | 0.000024 | 0.000017 | 0.000034 | 0.000041 | 0.000029 | 0.000018 | 0.000027 |
Vacuole | 0.005378 | 0.007382 | 0.058915 | 0.020613 | 0.009923 | 0.003463 | 0.009869 | 0.013604 | 0.008948 | 0.005163 | 0.014462 | 0.015805 |
Vacuole Periphery | 0.004067 | 0.005751 | 0.011150 | 0.011463 | 0.009333 | 0.003044 | 0.005977 | 0.007884 | 0.008204 | 0.004218 | 0.006461 | 0.009008 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.13 | 0.34 | 0.41 | 0.64 | 0.05 | -1.35 | 3.11 | -1.71 | 0.36 | -3.25 | -1.38 | 3.23 | -1.44 | 0.37 | -3.54 |
Bud Neck | 1.76 | -4.66 | -0.92 | -3.44 | 4.43 | 2.03 | -0.45 | -2.19 | -3.44 | -1.94 | 2.20 | -3.32 | -2.67 | -4.67 | 0.98 |
Bud Site | 0.98 | -1.16 | -2.07 | -2.59 | -1.31 | 1.15 | 2.92 | -3.49 | -3.74 | -4.03 | 0.98 | 2.45 | -4.17 | -4.47 | -4.81 |
Cell Periphery | -0.01 | -4.83 | -7.12 | -7.51 | -1.05 | 3.05 | -2.94 | -7.81 | -9.07 | -5.13 | 2.13 | -4.49 | -10.60 | -11.61 | -6.56 |
Cytoplasm | -2.81 | -0.01 | -2.31 | -1.78 | -2.32 | 0.18 | 1.94 | -1.11 | -1.22 | -2.21 | 0.21 | 1.94 | -1.62 | -1.83 | -2.88 |
Cytoplasmic Foci | 1.67 | 1.19 | 0.71 | -0.50 | -0.14 | 5.14 | 7.72 | 0.27 | -1.84 | -2.63 | 4.97 | 7.74 | 0.61 | -1.78 | -2.92 |
Eisosomes | 0.38 | -0.97 | 0.57 | 0.25 | 2.05 | -0.68 | -0.46 | 1.13 | 1.50 | 1.45 | -0.62 | -0.33 | 0.90 | 1.51 | 1.24 |
Endoplasmic Reticulum | -0.34 | 10.34 | 7.17 | 8.52 | -8.15 | -9.51 | -2.42 | 1.46 | 10.77 | 3.74 | -7.69 | 3.05 | 4.96 | 12.96 | 0.64 |
Endosome | 0.35 | -3.09 | -3.62 | -4.44 | 1.97 | 8.85 | 11.07 | -1.55 | -7.56 | -8.98 | 6.60 | 6.91 | -2.90 | -8.10 | -8.40 |
Golgi | -1.75 | -2.29 | -9.33 | -7.76 | -5.06 | 7.54 | 6.89 | 1.78 | -7.25 | -6.40 | 6.59 | 6.39 | -0.27 | -9.53 | -9.28 |
Lipid Particles | -0.45 | -1.91 | -3.91 | -3.90 | 1.20 | 7.42 | 8.92 | 3.08 | -5.02 | -7.01 | 6.51 | 4.27 | 0.86 | -5.96 | -3.69 |
Mitochondria | -0.14 | -1.88 | 1.46 | 1.78 | 3.33 | 1.10 | 2.36 | -0.86 | -2.00 | -3.26 | 0.76 | 2.19 | -0.33 | -1.17 | -2.74 |
Mitotic Spindle | 1.17 | 0.63 | 0.83 | -3.05 | 0.97 | 1.54 | 0.96 | -3.51 | -4.43 | -4.16 | 1.23 | 0.98 | 0.32 | -4.87 | -3.29 |
None | -1.01 | 1.79 | 1.05 | 2.05 | -3.45 | -0.82 | 6.35 | -1.25 | -0.18 | -3.37 | -1.26 | 6.00 | -0.69 | 0.59 | -3.63 |
Nuclear Periphery | 2.52 | -8.48 | -0.51 | -4.24 | 8.44 | 5.04 | -6.60 | 1.27 | -3.78 | 7.38 | 4.53 | -9.05 | 0.15 | -4.83 | 9.20 |
Nuclear Periphery Foci | -0.70 | -4.37 | -3.33 | -3.04 | 3.37 | 7.06 | 3.60 | -0.50 | -5.26 | -3.11 | 4.43 | -0.81 | -2.28 | -5.36 | -1.25 |
Nucleolus | -0.09 | -1.63 | 1.74 | 1.50 | 4.24 | 0.33 | 2.55 | -0.53 | -0.66 | -2.05 | -0.14 | 2.01 | 0.34 | 0.36 | -1.23 |
Nucleus | 0.30 | -1.04 | 0.10 | -0.28 | 1.73 | -0.24 | 2.13 | -1.45 | -0.64 | -3.00 | -0.18 | 2.07 | -1.33 | -0.77 | -3.25 |
Peroxisomes | 0.67 | 2.16 | 3.79 | 5.84 | 2.37 | 2.18 | 3.87 | 0.41 | -0.56 | -1.15 | 1.95 | 4.52 | 1.25 | 0.30 | -0.90 |
Vacuole | -2.15 | -7.30 | -7.32 | -6.25 | 4.80 | 6.50 | 0.10 | -2.95 | -8.71 | -2.93 | 4.39 | -3.91 | -6.18 | -10.11 | -1.45 |
Vacuole Periphery | -1.97 | -2.83 | -4.46 | -3.15 | -0.13 | 7.04 | 2.90 | 1.35 | -6.12 | -1.76 | 5.19 | 1.69 | -0.87 | -5.93 | -2.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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